MD03G1278900.v1.1

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009284
Physical Location & Seq
Reverse (-)
36080243 .. 36082238
1996 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1278900.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGCCTGTTGTTCAATATCCGGTTGGGTCTCTGCTGGCCACCCTGGCTCCATCATCCTCATCATCGGATCTCCGGCCTCCAAGCGAATCGTACCCGGAGAGACCCGGCGTGCCCAACTGTGTCTACTACATGCGAACCGGGTTCTGTGGATACGGTGGTCGGTGCCGGTACAACCATCCTCGTGATCGTGCTGCTGTAAGTCCTTGTTCTGGGAAATGTCGTCTTTACAACTGCTTGGATTTTATTGGTCGTGGCAGCTATAAGAGCTACAGGGGATTACCCAGAGAGAGTGGGGAAACCTGTATGTCAGTATATTTACAGACTGGGACCTGTAAATTTGGTGCGTCCTGCAAATTCCACCATCCAAAACATGGAGCTGGATCCTTAAACCGAGCTCCCCTAAATATTTATGGATACCCTCTCATCTACATATGGGGCGAGAATGAATGCTTCTACTATTTGAAAACGGGGCAATGCAAATTTGGCATAACCTGTAAATTCCATCATCCACAGCCTGCTGGAACAACAATACCGGCTTCTGTACCCCAATTTTATCCGTTGGTGCAGTCTCCTGTTCCTTTGGCAGAACAGTATGGGGGGGCATCCACCAGTTTGAGGGTGGCTAGGCCTCCACTATTAACTGGATCATATGTGCAAGGGACTTACTGTCCTGTCTTGATTCCTCCTCCTGGAGTTGTTCCGATTCAGGGTTGGAGTCCGTATTCGGCACCTCTTAGTGCTGTACCATCTCCTGGAGCTCAACCCACAGTTGGAGCAACTTCTTTATATGGAGTAACACAGCTATCTTCTCCAACACACGGACTTGCAAGCCCTTATACCTCCATACCCTCTTCTGTTGGTCCTTCTAGCAGCAACCCGAGTGAACAAGTTTTCCCAGAGAGGCCTGGTGAACTTGAATGCCAGTATTATCTGAAAACTGGGGACTGTAAATATGGACCATCTTGTAGGTATCATCATCCTCGGGATCGAACTGTACCGAGAATAACTTGTCTCCTTAGTCCGATGGGTCTCCCACTCCGTCCGGTATGCAAAGTTGTTTTATATTATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCCH

Protein Analysis

358

Amino Acids

38.84

Weight (kDa)

9.08

Isoelectric Point (pI)

54.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH PF00642 36 - 61 3.1e-08 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH PF00642 105 - 123 1.6e-07 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH PF00642 145 - 171 7.3e-08 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH PF00642 304 - 328 3.3e-09 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 162, 727
AccB7I CCANNNNNTGG 2 cut(s) 371, 752
AccI GTMKAC 1 cut(s) 123
AclWI GGATC 5 cut(s) 75, 375, 388, 652, 993
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 4 cut(s) 335, 353, 479, 497
AfaI GTAC 5 cut(s) 92, 170, 543, 744, 996
AfiI CCNNNNNNNGG 8 cut(s) 209, 371, 615, 689, 707, 752, 770, 818
AgsI TTSAA 3 cut(s) 14, 463, 917
AhdI GACNNNNNGTC 1 cut(s) 666
AjnI CCWGG 4 cut(s) 42, 688, 751, 904
AloI GAACNNNNNNTCC 2 cut(s) 876, 908
AluBI AGCT 6 cut(s) 258, 267, 377, 395, 758, 802
AluI AGCT 6 cut(s) 258, 267, 377, 395, 758, 802
Alw21I GWGCWC 2 cut(s) 397, 760
Alw26I GTCTC 5 cut(s) 33, 94, 573, 1016, 1034
AlwI GGATC 5 cut(s) 75, 375, 388, 652, 993
Ama87I CYCGRG 2 cut(s) 877, 981
AoxI GGCC 4 cut(s) 36, 74, 626, 902
ApeKI GCWGC 3 cut(s) 191, 255, 870
ApoI RAATTY 4 cut(s) 335, 353, 479, 497
AspS9I GGNCC 3 cut(s) 327, 860, 956
AsuC2I CCSGG 3 cut(s) 95, 105, 139
AsuHPI GGTGA 1 cut(s) 920
AvaI CYCGRG 2 cut(s) 877, 981
AvaII GGWCC 3 cut(s) 327, 860, 956
BaeGI GKGCMC 1 cut(s) 114
BaeI ACNNNNGTAYC 2 cut(s) 142, 175
BalI TGGCCA 1 cut(s) 38
BamHI GGATCC 1 cut(s) 380
BanI GGYRCC 2 cut(s) 162, 727
BanII GRGCYC 2 cut(s) 397, 760
BauI CACGAG 1 cut(s) 180
Bbv12I GWGCWC 2 cut(s) 397, 760
BbvI GCAGC 3 cut(s) 178, 267, 882
BccI CCATC 7 cut(s) 58, 183, 369, 510, 754, 967, 1018
BciT130I CCWGG 4 cut(s) 44, 690, 753, 906
BciVI GTATCC 2 cut(s) 143, 407
BcnI CCSGG 3 cut(s) 95, 105, 139
BcoDI GTCTC 5 cut(s) 33, 94, 573, 1016, 1034
BfaI CTAG 2 cut(s) 624, 867
BfmI CTRYAG 1 cut(s) 268
BfuI GTATCC 2 cut(s) 143, 407
BglI GCCNNNNNGGC 1 cut(s) 44
BisI GCNGC 3 cut(s) 192, 256, 871
BlsI GCNGC 3 cut(s) 193, 257, 872
Bme1390I CCNGG 7 cut(s) 44, 95, 105, 139, 690, 753, 906
Bme18I GGWCC 3 cut(s) 327, 860, 956
BmeRI GACNNNNNGTC 1 cut(s) 666
BmeT110I CYCGRG 2 cut(s) 877, 981
BmgT120I GGNCC 3 cut(s) 327, 860, 956
BmiI GGNNCC 5 cut(s) 48, 164, 328, 382, 729
BmrFI CCNGG 7 cut(s) 44, 95, 105, 139, 690, 753, 906
BmrI ACTGGG 2 cut(s) 333, 948
BmsI GCATC 1 cut(s) 611
BmuI ACTGGG 2 cut(s) 333, 948
BpmI CTGGAG 2 cut(s) 711, 774
BpuMI CCSGG 3 cut(s) 95, 105, 139
BsaI GGTCTC 3 cut(s) 33, 94, 1034
BsaJI CCNNGG 2 cut(s) 42, 980
BsaWI WCCGGW 2 cut(s) 19, 1042
BsaXI ACNNNNNCTCC 2 cut(s) 31, 61
Bsc4I CCNNNNNNNGG 8 cut(s) 209, 371, 615, 689, 707, 752, 770, 818
Bse118I RCCGGY 2 cut(s) 165, 532
Bse1I ACTGG 5 cut(s) 328, 609, 646, 922, 943
Bse3DI GCAATG 1 cut(s) 479
BseBI CCWGG 4 cut(s) 44, 690, 753, 906
BseDI CCNNGG 2 cut(s) 42, 980
BseGI GGATG 6 cut(s) 53, 175, 361, 505, 602, 976
BseLI CCNNNNNNNGG 8 cut(s) 209, 371, 615, 689, 707, 752, 770, 818
BseMI GCAATG 1 cut(s) 479
BseNI ACTGG 5 cut(s) 328, 609, 646, 922, 943
BseRI GAGGAG 1 cut(s) 675
BseSI GKGCMC 1 cut(s) 114
BseXI GCAGC 3 cut(s) 178, 267, 882
BsgI GTGCAG 1 cut(s) 584
BshFI GGCC 4 cut(s) 38, 76, 628, 904
BshNI GGYRCC 2 cut(s) 162, 727
BsiHKAI GWGCWC 2 cut(s) 397, 760
BsiHKCI CYCGRG 2 cut(s) 877, 981
BsiSI CCGG 8 cut(s) 20, 73, 95, 105, 138, 166, 533, 1043
BslFI GGGAC 3 cut(s) 340, 673, 956
BslI CCNNNNNNNGG 8 cut(s) 209, 371, 615, 689, 707, 752, 770, 818
BsmAI GTCTC 5 cut(s) 33, 94, 573, 1016, 1034
BsmFI GGGAC 3 cut(s) 340, 673, 956
BsmI GAATGC 2 cut(s) 452, 923
BsnI GGCC 4 cut(s) 38, 76, 628, 904
Bso31I GGTCTC 3 cut(s) 33, 94, 1034
BsoBI CYCGRG 2 cut(s) 877, 981
Bsp1286I GDGCHC 3 cut(s) 114, 397, 760
Bsp143I GATC 5 cut(s) 67, 184, 380, 644, 985
BspANI GGCC 4 cut(s) 38, 76, 628, 904
BspLI GGNNCC 5 cut(s) 48, 164, 328, 382, 729
BspPI GGATC 5 cut(s) 75, 375, 388, 652, 993
BspT107I GGYRCC 2 cut(s) 162, 727
BspTNI GGTCTC 3 cut(s) 33, 94, 1034
BsrDI GCAATG 1 cut(s) 479
BsrFI RCCGGY 2 cut(s) 165, 532
BsrI ACTGG 5 cut(s) 328, 609, 646, 922, 943
BssAI RCCGGY 2 cut(s) 165, 532
BssECI CCNNGG 2 cut(s) 42, 980
BssMI GATC 5 cut(s) 67, 184, 380, 644, 985
BssSI CACGAG 1 cut(s) 180
Bst2BI CACGAG 1 cut(s) 180
Bst2UI CCWGG 4 cut(s) 44, 690, 753, 906
Bst4CI ACNGT 7 cut(s) 119, 155, 591, 668, 769, 947, 994
Bst6I CTCTTC 1 cut(s) 856
BstC8I GCNNGC 4 cut(s) 36, 110, 516, 829
BstDEI CTNAG 2 cut(s) 734, 1016
BstF5I GGATG 6 cut(s) 53, 175, 361, 505, 602, 976
BstKTI GATC 5 cut(s) 70, 187, 383, 647, 988
BstMAI GTCTC 5 cut(s) 33, 94, 573, 1016, 1034
BstMBI GATC 5 cut(s) 67, 184, 380, 644, 985
BstMWI GCNNNNNNNGC 3 cut(s) 44, 264, 483
BstNI CCWGG 4 cut(s) 44, 690, 753, 906
BstNSI RCATGY 1 cut(s) 133
BstSCI CCNGG 7 cut(s) 42, 93, 103, 137, 688, 751, 904
BstSFI CTRYAG 1 cut(s) 268
BstSLI GKGCMC 1 cut(s) 114
BstV1I GCAGC 3 cut(s) 178, 267, 882
BstX2I RGATCY 2 cut(s) 67, 380
BstYI RGATCY 2 cut(s) 67, 380
BsuI GTATCC 2 cut(s) 143, 407
BsuRI GGCC 4 cut(s) 38, 76, 628, 904
BtsCI GGATG 6 cut(s) 53, 175, 361, 505, 602, 976
Cac8I GCNNGC 4 cut(s) 36, 110, 516, 829
Cfr10I RCCGGY 2 cut(s) 165, 532
Cfr13I GGNCC 3 cut(s) 327, 860, 956
CseI GACGC 1 cut(s) 333
Csp6I GTAC 5 cut(s) 91, 169, 542, 743, 995
CviAII CATG 2 cut(s) 130, 371
CviQI GTAC 5 cut(s) 91, 169, 542, 743, 995
DdeI CTNAG 2 cut(s) 734, 1016
DpnI GATC 5 cut(s) 69, 186, 382, 646, 987
DpnII GATC 5 cut(s) 67, 184, 380, 644, 985
DriI GACNNNNNGTC 1 cut(s) 666
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 856
Eam1105I GACNNNNNGTC 1 cut(s) 666
EarI CTCTTC 1 cut(s) 856
Ecl136II GAGCTC 2 cut(s) 395, 758
Eco147I AGGCCT 2 cut(s) 628, 904
Eco24I GRGCYC 2 cut(s) 397, 760
Eco31I GGTCTC 3 cut(s) 33, 94, 1034
Eco47I GGWCC 3 cut(s) 327, 860, 956
Eco53kI GAGCTC 2 cut(s) 395, 758
Eco88I CYCGRG 2 cut(s) 877, 981
EcoICRI GAGCTC 2 cut(s) 395, 758
EcoO109I RGGNCCY 1 cut(s) 327
EcoRII CCWGG 4 cut(s) 42, 688, 751, 904
EcoT38I GRGCYC 2 cut(s) 397, 760
FaeI CATG 2 cut(s) 133, 374
FaqI GGGAC 3 cut(s) 340, 673, 956
FatI CATG 2 cut(s) 129, 370
FauNDI CATATG 2 cut(s) 431, 649
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 3 cut(s) 192, 256, 871
FokI GGATG 6 cut(s) 40, 162, 348, 492, 589, 963
FriOI GRGCYC 2 cut(s) 397, 760
Fsp4HI GCNGC 3 cut(s) 192, 256, 871
FspBI CTAG 2 cut(s) 624, 867
GluI GCNGC 3 cut(s) 192, 256, 871
GsuI CTGGAG 2 cut(s) 711, 774
HaeIII GGCC 4 cut(s) 38, 76, 628, 904
HapII CCGG 8 cut(s) 20, 73, 95, 105, 138, 166, 533, 1043
HgaI GACGC 1 cut(s) 333
Hin1II CATG 2 cut(s) 133, 374
HinfI GANTC 4 cut(s) 86, 679, 703, 715
HpaII CCGG 8 cut(s) 20, 73, 95, 105, 138, 166, 533, 1043
HphI GGTGA 1 cut(s) 920
Hpy166II GTNNAC 3 cut(s) 124, 884, 911
Hpy188I TCNGA 4 cut(s) 67, 702, 933, 1023
Hpy188III TCNNGA 3 cut(s) 182, 676, 983
Hpy8I GTNNAC 3 cut(s) 124, 884, 911
HpyAV CCTTC 1 cut(s) 873
HpyCH4III ACNGT 7 cut(s) 119, 155, 591, 668, 769, 947, 994
HpyCH4V TGCA 6 cut(s) 351, 477, 565, 655, 827, 1050
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 264, 483
HpyF3I CTNAG 2 cut(s) 734, 1016
Hsp92II CATG 2 cut(s) 133, 374
Kzo9I GATC 5 cut(s) 67, 184, 380, 644, 985
LmnI GCTCC 5 cut(s) 52, 374, 400, 755, 773
Lsp1109I GCAGC 3 cut(s) 178, 267, 882
LweI GCATC 1 cut(s) 611
MaeI CTAG 2 cut(s) 624, 867
MaeIII GTNAC 1 cut(s) 793
MalI GATC 5 cut(s) 69, 186, 382, 646, 987
MboI GATC 5 cut(s) 67, 184, 380, 644, 985
MboII GAAGA 2 cut(s) 798, 843
MflI RGATCY 2 cut(s) 67, 380
MhlI GDGCHC 3 cut(s) 114, 397, 760
MlsI TGGCCA 1 cut(s) 38
MluCI AATT 5 cut(s) 335, 353, 479, 497, 548
MluNI TGGCCA 1 cut(s) 38
MlyI GAGTC 1 cut(s) 724
MmeI TCCRAC 3 cut(s) 692, 751, 836
Mox20I TGGCCA 1 cut(s) 38
MscI TGGCCA 1 cut(s) 38
MseI TTAA 2 cut(s) 386, 638
MslI CAYNNNNRTG 1 cut(s) 180
Msp20I TGGCCA 1 cut(s) 38
MspI CCGG 8 cut(s) 20, 73, 95, 105, 138, 166, 533, 1043
MspR9I CCNGG 7 cut(s) 44, 95, 105, 139, 690, 753, 906
Mva1269I GAATGC 2 cut(s) 452, 923
MvaI CCWGG 4 cut(s) 44, 690, 753, 906
MwoI GCNNNNNNNGC 3 cut(s) 44, 264, 483
NciI CCSGG 3 cut(s) 95, 105, 139
NdeI CATATG 2 cut(s) 431, 649
NdeII GATC 5 cut(s) 67, 184, 380, 644, 985
NlaIII CATG 2 cut(s) 133, 374
NlaIV GGNNCC 5 cut(s) 48, 164, 328, 382, 729
NspI RCATGY 1 cut(s) 133
PceI AGGCCT 2 cut(s) 628, 904
PctI GAATGC 2 cut(s) 452, 923
PfeI GAWTC 3 cut(s) 86, 679, 703
PflMI CCANNNNNTGG 2 cut(s) 371, 752
PfoI TCCNGGA 2 cut(s) 688, 751
PkrI GCNGC 3 cut(s) 193, 257, 872
PleI GAGTC 1 cut(s) 723
PpsI GAGTC 1 cut(s) 723
PpuMI RGGWCCY 1 cut(s) 327
Psp124BI GAGCTC 2 cut(s) 397, 760
Psp5II RGGWCCY 1 cut(s) 327
Psp6I CCWGG 4 cut(s) 42, 688, 751, 904
PspGI CCWGG 4 cut(s) 42, 688, 751, 904
PspN4I GGNNCC 5 cut(s) 48, 164, 328, 382, 729
PspPI GGNCC 3 cut(s) 327, 860, 956
PspPPI RGGWCCY 1 cut(s) 327
PsuI RGATCY 2 cut(s) 67, 380
RsaI GTAC 5 cut(s) 92, 170, 543, 744, 996
RsaNI GTAC 5 cut(s) 91, 169, 542, 743, 995
RseI CAYNNNNRTG 1 cut(s) 180
SacI GAGCTC 2 cut(s) 397, 760
SaqAI TTAA 2 cut(s) 386, 638
SatI GCNGC 3 cut(s) 192, 256, 871
Sau3AI GATC 5 cut(s) 67, 184, 380, 644, 985
Sau96I GGNCC 3 cut(s) 327, 860, 956
SchI GAGTC 1 cut(s) 724
ScrFI CCNGG 7 cut(s) 44, 95, 105, 139, 690, 753, 906
SduI GDGCHC 3 cut(s) 114, 397, 760
SfaNI GCATC 1 cut(s) 611
SfcI CTRYAG 1 cut(s) 268
SinI GGWCC 3 cut(s) 327, 860, 956
SmiMI CAYNNNNRTG 1 cut(s) 180
Sse9I AATT 5 cut(s) 335, 353, 479, 497, 548
SseBI AGGCCT 2 cut(s) 628, 904
SspI AATATT 1 cut(s) 406
SspMI CTAG 2 cut(s) 624, 867
SstI GAGCTC 2 cut(s) 397, 760
StuI AGGCCT 2 cut(s) 628, 904
StyD4I CCNGG 7 cut(s) 42, 93, 103, 137, 688, 751, 904
TaaI ACNGT 7 cut(s) 119, 155, 591, 668, 769, 947, 994
TaqI TCGA 1 cut(s) 988
TasI AATT 5 cut(s) 335, 353, 479, 497, 548
TfiI GAWTC 3 cut(s) 86, 679, 703
Tru1I TTAA 2 cut(s) 386, 638
Tru9I TTAA 2 cut(s) 386, 638
TseI GCWGC 3 cut(s) 191, 255, 870
TspDTI ATGAA 1 cut(s) 459
TspGWI ACGGA 4 cut(s) 546, 708, 834, 1028
Van91I CCANNNNNTGG 2 cut(s) 371, 752
VpaK11BI GGWCC 3 cut(s) 327, 860, 956
XapI RAATTY 4 cut(s) 335, 353, 479, 497
XceI RCATGY 1 cut(s) 133
XcmI CCANNNNNNNNNTGG 2 cut(s) 368, 616
XmiI GTMKAC 1 cut(s) 123
XspI CTAG 2 cut(s) 624, 867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.