Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0015967

Physical Location & Seq

Genomic Coordinates Forward (+)

36667170 .. 36673664

Gene Structure 6495 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1287700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3045 bp

ATGGCGTCACCTCCGTCCCCGACGTCGTCGGTCGGCAGCTCCTCGTCGTCGTCGTTTTCCAACTCTAAATTCTCGTCTCTTCCCGTAATGGCGCTCCGGGAGAAGATCGTCGAGAAAATCCTTGATAATCGCGTCACTCTCATCGTCGGCGAGACCGGTTGCGGGAAAAGCTCGCAAGTTCCTCAGTTTCTTCTGGAAGCGAATTTGAAGCCAATTATATGCACACAGCCGAGGAGATTTGCAGTTGTTGCTGTTGCGAAAATGGTTGCGAAAGCTCGGAATTGCGAGCTTGGTGGAGAGGTTGGATATCACATTGGCCATTCAAAGCACTTATCGCCAAGGTCTTCGATTGTTTTTAAAACTGCTGGAGTTTTATTGGATGAAATGCGAGATAAGGGGATGCATGCACTTGATTACAAGGTCATTGTTCTTGATGAAGTGCATGAAAGATCTGTTGAATCCGATCTTGTTCTTGTTTGTGTTAAGCAGTTTCTGATGAGGAACAACAACTTGAGGGTTGTGTTGATGTCTGCAACTGCTGATATTGCGAGATACAGGGATTACTTTAAGGATCTTGGCAGGGGTGAGCGAGTGGAAGTGCTTGCAATCCCTTCCACCGGCCAAAATACCATTTTTCAGAAAAGGGTTTCTTATCTTGAAGAGGTCACCGATCTTCTCAACATCGACTCAGAGTTGATTCCTTCAAAATATTGTCCAGGTGCAAGTCCTTCAATGGCTCAAGCCGATATTAAGCCTGAAGTGCACCAACTTATCCATCGTTTGGTGTTGCATATTCACGACCACGAGCCAGACATTGAGAAGAGCATTTTGATTTTTCTTCCTACATACTATGCACTGGTGCAGCAATGGTTCCTTCTGAAGCCATTTAGTTCATCTTTTAAAGTTCATATTTTACATAGCAGCATTGACACTGAACAAGCTCTCATGAGTATGAAGATATGGAAATCCCATCGTAAAGTGATACTGGCTACCAATATTGCAGAATCATCAGTAACAATACCCAAGGTGGCCTATGTTATTGATTCATGCCGATCTTTACAAGTCTTCTGGAACAGTTTTCAGAAGAAGGAAGCTGCGAAGCTTGTTTGGGTTTCTCATTCTCAGGCTGAGCAGCGTAGAGGAAGAACTGGTCGAACTTGTGATGGCCAGATTTATAGGTTGGTTACTAGACCATTTTTCAACCTGATTGACAAATACGAGGGTCCGTCTATACTGAGGTTATCATTGAGGCTGCAAGTGCTTCAGATATGCTGTGCTGAATCTAAAGCCATTAATGATCCCAAAGGCTTATTGCAGAAGGCTCTAGATCCACCATATCCTGAAGTTATTGAAGATGCATTGGATTTGCTTGTTCAAATGAAAGCACTGGAGAAAACATTTCAAAGGGGCCGGTATGAGCCTACATTTTATGGGCGCTTGCTTGCCAGTTTCTCCTTGTCTTTTGATGCTTCTCTGCTTGTACTCAAGTTTGGAGATATTGGAATGCTGCGCGAAGGAATTCTTCTGGGAATATTAATGGATACGCAGCCTTTACCTATTGTTCGTCCTTTTGGAGATGAAATTTTGTGTTCAGAATATGCTGACAGCTACTTTTGTGGAGCTGAGAGCAATACTGCCCTAAAGGGTAGGAAGGAGACTATATTCATGGCAAATTTGTGTGCATTTCAATTTTGGCAACGTGTTTTCAAGGATAAGCAACGTGTGGAGCGATTGAAGCAACTTATGTTTGATGAGACAACAGCAACTACGGTACTGCTTCCAAAGGTTGAAGAAGATTGGTGCTCTTTGCATAATCTTGTGCAGTCGTCACTAAAACATGTATCTGAAATATATGAAGATATACTAGATTCAGTGCACCGGTTTCGGCCCAAATTTTTGTCTACATCTAATGGACTTCCATCCTACTATGACCCTTATGAATTTGAGCATACATGCCTCCTTGCATATCAAGAACCAAAAGAAGCTACAGATGCCCTTTCTTCAGCTGATCAGCACCTTGAGCCATCTGGTGAAACAAAGAAATGTATTTCTGTACCTTTTGTTGCTCCAGATACCTTCCAAAATAATGACGTGGCTGAAAAGTTGGCAACAATAATCAAACAGATACGAGTTCAGTATACAGAAGATGTATCTGCTAATCAGGTTTTGGATGCTGATGAGCCTCCTATTTGTATATATTTTATCAATGGGGGCTGCAGAAATGGCAGTCAATGCCAGTTTTCTCATTCACTTGGAGCAAGGAGACCCCCATGCAAATTTTTCTCTTCGCCACAGGGTTGTCGATATGGTGACAAATGCTTATTTTCTCACGATGAGGGTCCACCAATTCCATCACCTAGCTCAACTTTATGCATACCAGAAGGTGGTGAAGCCGAAGCTACATCACTTCTACAGTTGTTTCCGACTTCAGATGGATGCATTCTCGTATTGGATGACACCAATCTAGAATTTTCCTCAAATTTAGCTAAATTTTGTGATCCATCGACAGTAGTATCCACCACCAGTTTGTCGGACACAACCATATTTGACCCGTCACTGACCGGTGTCAAAATTTTGTGGGGGCTGCAGCACCCATATGAGACAATTGTGTCCAAAGAAGGAGAGAACCAGATTCCATGGAACGAAGTTAAGTGCGTGCTATGGTTTCCTGATTTTGATAGCTACAGTGAGAATCTGGACAGACAGAAGATTCTCTTGCGGAATTTCTTCGAGTATCTTGCCATCCGAATGTTAACTGATGCCTTGTACAAAGTTCGAGTTGTACTCACTATGAACAACATCAGGTTTTCTCAGCTACAGGTTGAAAAGTTCGGCAGGGAAAGCTTCTTCTTCCTGTCAGAGTCGTTTCCATTCGATCACCACAGCTTCGGGGAGTTGCCAGACAAAATCACGACAAAGAAGCCGATGGTGGTTTCGAGGCCAATTTCATATGTTTTCGATTTTCACAAGCCTTCGAACTTTCAGTTTGACAACAATGCAACCGGAGTTAGCGGTTATGCTCAATACATCCAGGAAGATTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C zf-CCCH

Protein Analysis

1015

Amino Acids

114.54

Weight (kDa)

6.08

Isoelectric Point (pI)

43.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	34 - 181	2.1e-07	DEAD/DEAH box helicase
Helicase_C	PF00271	270 - 386	2.8e-12	Helicase conserved C-terminal domain
zf-CCCH_4	PF18044	756 - 778	6.7e-07	CCCH-type zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0015967)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47680
fragaria_vesca	FvH4_2g25691
malus_domestica	MD03G1287700.v1.1
prunus_persica	Prupe.8G260900_v2.0.a1
pyrus_communis	pycom03g22780
rosa_chinensis	RchiOBHm_Chr6g0293951
rosa_laevigata	RLG00000011913
rosa_multiflora	Rmu_sc0005647.1_g000006
rosa_roxburghii	Rroxscaffold_7G00173800
rosa_rugosa	Rorug06G0239500
rosa_samantha	Rh6AG352600 Rh6BG359500 Rh6CG366800 Rh6DG353200
rosa_wichuraiana	Rw6G030710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2608
AatII	GACGTC	1 cut(s)	26
AccB7I	CCANNNNNTGG	2 cut(s)	781, 2384
AccI	GTMKAC	2 cut(s)	1901, 2138
AccII	CGCG	2 cut(s)	132, 1512
AciI	CCGC	3 cut(s)	162, 2719, 3012
AclWI	GGATC	4 cut(s)	579, 1292, 1322, 2492
AcoI	YGGCCR	3 cut(s)	316, 619, 1165
AcuI	CTGAAG	6 cut(s)	777, 899, 1247, 1362, 1986, 2412
AcyI	GRCGYC	2 cut(s)	5, 23
AfaI	GTAC	5 cut(s)	1482, 1773, 2055, 2768, 2784
AfiI	CCNNNNNNNGG	4 cut(s)	162, 617, 781, 2384
AflIII	ACRYGT	3 cut(s)	1699, 1720, 1837
AgeI	ACCGGT	3 cut(s)	155, 1878, 2561
AjiI	CACGTC	1 cut(s)	2092
AjnI	CCWGG	2 cut(s)	715, 3030
AjuI	GAANNNNNNNTTGG	2 cut(s)	53, 85
Alw21I	GWGCWC	3 cut(s)	765, 1805, 1878
Alw26I	GTCTC	6 cut(s)	81, 146, 1649, 1748, 2257, 2594
Alw44I	GTGCAC	2 cut(s)	761, 1874
AlwI	GGATC	4 cut(s)	579, 1292, 1322, 2492
AlwNI	CAGNNNCTG	1 cut(s)	493
AoxI	GGCC	7 cut(s)	316, 619, 1029, 1165, 1408, 1886, 2939
ApaLI	GTGCAC	2 cut(s)	761, 1874
AseI	ATTAAT	2 cut(s)	1293, 1535
AsiGI	ACCGGT	3 cut(s)	155, 1878, 2561
Asp700I	GAANNNNTTC	2 cut(s)	1518, 2726
AspLEI	GCGC	3 cut(s)	94, 1437, 1512
AspS9I	GGNCC	4 cut(s)	1223, 1408, 1887, 2339
AsuC2I	CCSGG	1 cut(s)	98
AsuHPI	GGTGA	7 cut(s)	596, 658, 2042, 2321, 2346, 2399, 2870
AsuII	TTCGAA	1 cut(s)	2975
AvaII	GGWCC	2 cut(s)	1223, 2339
BaeGI	GKGCMC	2 cut(s)	765, 1878
BaeI	ACNNNNGTAYC	2 cut(s)	2496, 2529
BalI	TGGCCA	2 cut(s)	318, 1167
BauI	CACGAG	1 cut(s)	803
BbsI	GAAGAC	2 cut(s)	336, 1057
Bbv12I	GWGCWC	3 cut(s)	765, 1805, 1878
BcgI	CGANNNNNNTGC	4 cut(s)	1865, 1899, 2720, 2754
BciT130I	CCWGG	2 cut(s)	717, 3032
BciVI	GTATCC	2 cut(s)	1534, 2524
BclI	TGATCA	1 cut(s)	2008
BcnI	CCSGG	1 cut(s)	98
BcoDI	GTCTC	6 cut(s)	81, 146, 1649, 1748, 2257, 2594
BfaI	CTAG	5 cut(s)	1188, 1325, 1865, 2358, 2465
BfmI	CTRYAG	6 cut(s)	1986, 2215, 2411, 2585, 2683, 2816
BfoI	RGCGCY	2 cut(s)	95, 1438
BfuI	GTATCC	2 cut(s)	1534, 2524
BglII	AGATCT	1 cut(s)	449
BlpI	GCTNAGC	1 cut(s)	1128
Bme1390I	CCNGG	3 cut(s)	98, 717, 3032
Bme18I	GGWCC	2 cut(s)	1223, 2339
BmgBI	CACGTC	1 cut(s)	2092
BmgT120I	GGNCC	4 cut(s)	1223, 1408, 1887, 2339
BmiI	GGNNCC	4 cut(s)	872, 1224, 1409, 2340
BmrFI	CCNGG	3 cut(s)	98, 717, 3032
BmsI	GCATC	7 cut(s)	390, 1345, 1456, 1981, 2161, 2426, 2749
BoxI	GACNNNNGTC	1 cut(s)	2564
BpiI	GAAGAC	2 cut(s)	336, 1057
BplI	GAGNNNNNCTC	2 cut(s)	2769, 2801
BpmI	CTGGAG	3 cut(s)	387, 1409, 2052
Bpu1102I	GCTNAGC	1 cut(s)	1128
Bpu14I	TTCGAA	1 cut(s)	2975
BpuEI	CTTGAG	4 cut(s)	532, 723, 1469, 2039
BpuMI	CCSGG	1 cut(s)	98
BsaHI	GRCGYC	2 cut(s)	5, 23
BsaI	GGTCTC	2 cut(s)	146, 2257
BsaJI	CCNNGG	4 cut(s)	230, 338, 1023, 2636
BsaWI	WCCGGW	4 cut(s)	155, 1878, 2561, 3002
Bsc4I	CCNNNNNNNGG	4 cut(s)	162, 617, 781, 2384
Bse118I	RCCGGY	5 cut(s)	155, 617, 1410, 1878, 2561
Bse1I	ACTGG	7 cut(s)	861, 990, 1153, 1392, 1446, 2236, 2523
Bse3DI	GCAATG	1 cut(s)	872
BseBI	CCWGG	2 cut(s)	717, 3032
BseDI	CCNNGG	4 cut(s)	230, 338, 1023, 2636
BseGI	GGATG	8 cut(s)	385, 405, 1919, 2176, 2441, 2458, 2742, 3027
BseLI	CCNNNNNNNGG	4 cut(s)	162, 617, 781, 2384
BseMI	GCAATG	1 cut(s)	872
BseMII	CTCAG	7 cut(s)	197, 702, 1119, 1136, 1226, 1614, 2825
BseNI	ACTGG	7 cut(s)	861, 990, 1153, 1392, 1446, 2236, 2523
BseRI	GAGGAG	2 cut(s)	31, 247
BseSI	GKGCMC	2 cut(s)	765, 1878
BsgI	GTGCAG	2 cut(s)	881, 1841
Bsh1236I	CGCG	2 cut(s)	132, 1512
Bsh1285I	CGRYCG	1 cut(s)	33
BshFI	GGCC	7 cut(s)	318, 621, 1031, 1167, 1410, 1888, 2941
BshTI	ACCGGT	3 cut(s)	155, 1878, 2561
BsiEI	CGRYCG	1 cut(s)	33
BsiHKAI	GWGCWC	3 cut(s)	765, 1805, 1878
BsiSI	CCGG	7 cut(s)	97, 156, 618, 1411, 1879, 2562, 3003
BslI	CCNNNNNNNGG	4 cut(s)	162, 617, 781, 2384
BsmAI	GTCTC	6 cut(s)	81, 146, 1649, 1748, 2257, 2594
BsmBI	CGTCTC	1 cut(s)	81
BsmI	GAATGC	2 cut(s)	1509, 2439
BsnI	GGCC	7 cut(s)	318, 621, 1031, 1167, 1410, 1888, 2941
Bso31I	GGTCTC	2 cut(s)	146, 2257
Bsp119I	TTCGAA	1 cut(s)	2975
Bsp1286I	GDGCHC	3 cut(s)	765, 1805, 1878
Bsp1407I	TGTACA	1 cut(s)	2766
Bsp1720I	GCTNAGC	1 cut(s)	1128
Bsp19I	CCATGG	1 cut(s)	2636
BspACI	CCGC	3 cut(s)	162, 2719, 3012
BspANI	GGCC	7 cut(s)	318, 621, 1031, 1167, 1410, 1888, 2941
BspCNI	CTCAG	7 cut(s)	196, 701, 1120, 1135, 1227, 1615, 2824
BspFNI	CGCG	2 cut(s)	132, 1512
BspHI	TCATGA	1 cut(s)	945
BspLI	GGNNCC	4 cut(s)	872, 1224, 1409, 2340
BspMAI	CTGCAG	2 cut(s)	2219, 2589
BspPI	GGATC	4 cut(s)	579, 1292, 1322, 2492
BspQI	GCTCTTC	1 cut(s)	815
BspT104I	TTCGAA	1 cut(s)	2975
BspTNI	GGTCTC	2 cut(s)	146, 2257
BsrDI	GCAATG	1 cut(s)	872
BsrFI	RCCGGY	5 cut(s)	155, 617, 1410, 1878, 2561
BsrGI	TGTACA	1 cut(s)	2766
BsrI	ACTGG	7 cut(s)	861, 990, 1153, 1392, 1446, 2236, 2523
BssAI	RCCGGY	5 cut(s)	155, 617, 1410, 1878, 2561
BssECI	CCNNGG	4 cut(s)	230, 338, 1023, 2636
BssNAI	GTATAC	1 cut(s)	2139
BssNI	GRCGYC	2 cut(s)	5, 23
BssSI	CACGAG	1 cut(s)	803
BssT1I	CCWWGG	3 cut(s)	338, 1023, 2636
Bst1107I	GTATAC	1 cut(s)	2139
Bst2BI	CACGAG	1 cut(s)	803
Bst2UI	CCWGG	2 cut(s)	717, 3032
Bst4CI	ACNGT	5 cut(s)	1076, 1771, 2415, 2509, 2687
Bst6I	CTCTTC	4 cut(s)	84, 654, 815, 2290
BstACI	GRCGYC	2 cut(s)	5, 23
BstAPI	GCANNNNNTGC	2 cut(s)	248, 2232
BstAUI	TGTACA	1 cut(s)	2766
BstBI	TTCGAA	1 cut(s)	2975
BstC8I	GCNNGC	7 cut(s)	173, 287, 405, 603, 1439, 1443, 2657
BstDEI	CTNAG	7 cut(s)	183, 688, 1122, 1128, 1235, 1623, 2811
BstDSI	CCRYGG	1 cut(s)	2636
BstEII	GGTNACC	1 cut(s)	664
BstF5I	GGATG	8 cut(s)	385, 405, 1919, 2176, 2441, 2458, 2742, 3027
BstFNI	CGCG	2 cut(s)	132, 1512
BstH2I	RGCGCY	2 cut(s)	95, 1438
BstHHI	GCGC	3 cut(s)	94, 1437, 1512
BstMAI	GTCTC	6 cut(s)	81, 146, 1649, 1748, 2257, 2594
BstMCI	CGRYCG	1 cut(s)	33
BstNI	CCWGG	2 cut(s)	717, 3032
BstNSI	RCATGY	3 cut(s)	407, 1841, 1956
BstPAI	GACNNNNGTC	1 cut(s)	2564
BstPI	GGTNACC	1 cut(s)	664
BstSCI	CCNGG	3 cut(s)	96, 715, 3030
BstSFI	CTRYAG	6 cut(s)	1986, 2215, 2411, 2585, 2683, 2816
BstSLI	GKGCMC	2 cut(s)	765, 1878
BstUI	CGCG	2 cut(s)	132, 1512
BstV2I	GAAGAC	2 cut(s)	336, 1057
BstX2I	RGATCY	3 cut(s)	449, 571, 1327
BstYI	RGATCY	3 cut(s)	449, 571, 1327
BstZ17I	GTATAC	1 cut(s)	2139
BsuI	GTATCC	2 cut(s)	1534, 2524
BsuRI	GGCC	7 cut(s)	318, 621, 1031, 1167, 1410, 1888, 2941
BtgI	CCRYGG	1 cut(s)	2636
BtrI	CACGTC	1 cut(s)	2092
BtsCI	GGATG	8 cut(s)	385, 405, 1919, 2176, 2441, 2458, 2742, 3027
BtsIMutI	CAGTG	6 cut(s)	854, 930, 1385, 1878, 2555, 2692
Cac8I	GCNNGC	7 cut(s)	173, 287, 405, 603, 1439, 1443, 2657
CaiI	CAGNNNCTG	1 cut(s)	493
CciI	TCATGA	1 cut(s)	945
CfoI	GCGC	3 cut(s)	94, 1437, 1512
Cfr10I	RCCGGY	5 cut(s)	155, 617, 1410, 1878, 2561
Cfr13I	GGNCC	4 cut(s)	1223, 1408, 1887, 2339
CseI	GACGC	1 cut(s)	121
Csp6I	GTAC	5 cut(s)	1481, 1772, 2054, 2767, 2783
CspAI	ACCGGT	3 cut(s)	155, 1878, 2561
CviAII	CATG	9 cut(s)	404, 443, 946, 1047, 1666, 1838, 1953, 2271, 2637
CviQI	GTAC	5 cut(s)	1481, 1772, 2054, 2767, 2783
DdeI	CTNAG	7 cut(s)	183, 688, 1122, 1128, 1235, 1623, 2811
DraI	TTTAAA	2 cut(s)	358, 901
DrdI	GACNNNNNNGTC	1 cut(s)	2608
DseDI	GACNNNNNNGTC	1 cut(s)	2608
EaeI	YGGCCR	3 cut(s)	316, 619, 1165
Eam1104I	CTCTTC	4 cut(s)	84, 654, 815, 2290
EarI	CTCTTC	4 cut(s)	84, 654, 815, 2290
Eco130I	CCWWGG	3 cut(s)	338, 1023, 2636
Eco31I	GGTCTC	2 cut(s)	146, 2257
Eco32I	GATATC	1 cut(s)	308
Eco47I	GGWCC	2 cut(s)	1223, 2339
Eco57I	CTGAAG	6 cut(s)	777, 899, 1247, 1362, 1986, 2412
Eco91I	GGTNACC	1 cut(s)	664
EcoO65I	GGTNACC	1 cut(s)	664
EcoRI	GAATTC	1 cut(s)	1518
EcoRII	CCWGG	2 cut(s)	715, 3030
EcoRV	GATATC	1 cut(s)	308
EcoT14I	CCWWGG	3 cut(s)	338, 1023, 2636
EcoT22I	ATGCAT	4 cut(s)	405, 1360, 2375, 2441
ErhI	CCWWGG	3 cut(s)	338, 1023, 2636
Esp3I	CGTCTC	1 cut(s)	81
FaeI	CATG	9 cut(s)	407, 446, 949, 1050, 1669, 1841, 1956, 2274, 2640
FalI	AAGNNNNNCTT	6 cut(s)	634, 666, 1506, 1538, 2699, 2731
FatI	CATG	9 cut(s)	403, 442, 945, 1046, 1665, 1837, 1952, 2270, 2636
FauI	CCCGC	1 cut(s)	155
FauNDI	CATATG	2 cut(s)	2596, 2950
FbaI	TGATCA	1 cut(s)	2008
FblI	GTMKAC	2 cut(s)	1901, 2138
FokI	GGATG	8 cut(s)	392, 412, 1906, 2183, 2448, 2465, 2729, 3014
FspBI	CTAG	5 cut(s)	1188, 1325, 1865, 2358, 2465
GlaI	GCGC	3 cut(s)	93, 1436, 1511
GsuI	CTGGAG	3 cut(s)	387, 1409, 2052
HaeII	RGCGCY	2 cut(s)	95, 1438
HaeIII	GGCC	7 cut(s)	318, 621, 1031, 1167, 1410, 1888, 2941
HapII	CCGG	7 cut(s)	97, 156, 618, 1411, 1879, 2562, 3003
HgaI	GACGC	1 cut(s)	121
HhaI	GCGC	3 cut(s)	94, 1437, 1512
Hin1I	GRCGYC	2 cut(s)	5, 23
Hin1II	CATG	9 cut(s)	407, 446, 949, 1050, 1669, 1841, 1956, 2274, 2640
Hin6I	GCGC	3 cut(s)	92, 1435, 1510
HinP1I	GCGC	3 cut(s)	92, 1435, 1510
HincII	GTYRAC	1 cut(s)	2754
HindII	GTYRAC	1 cut(s)	2754
HindIII	AAGCTT	2 cut(s)	1100, 2842
HpaI	GTTAAC	1 cut(s)	2754
HpaII	CCGG	7 cut(s)	97, 156, 618, 1411, 1879, 2562, 3003
HphI	GGTGA	7 cut(s)	596, 658, 2042, 2321, 2346, 2399, 2870
Hpy166II	GTNNAC	6 cut(s)	763, 1876, 1902, 2139, 2342, 2754
Hpy8I	GTNNAC	6 cut(s)	763, 1876, 1902, 2139, 2342, 2754
Hpy99I	CGWCG	8 cut(s)	25, 28, 31, 49, 52, 55, 113, 149
HpyCH4III	ACNGT	5 cut(s)	1076, 1771, 2415, 2509, 2687
HpyCH4IV	ACGT	4 cut(s)	23, 1699, 1720, 2091
HpyF3I	CTNAG	7 cut(s)	183, 688, 1122, 1128, 1235, 1623, 2811
HpySE526I	ACGT	4 cut(s)	23, 1699, 1720, 2091
Hsp92I	GRCGYC	2 cut(s)	5, 23
Hsp92II	CATG	9 cut(s)	407, 446, 949, 1050, 1669, 1841, 1956, 2274, 2640
HspAI	GCGC	3 cut(s)	92, 1435, 1510
Ksp22I	TGATCA	1 cut(s)	2008
KspAI	GTTAAC	1 cut(s)	2754
LguI	GCTCTTC	1 cut(s)	815
LmnI	GCTCC	6 cut(s)	44, 99, 1619, 1726, 2071, 2255
LweI	GCATC	7 cut(s)	390, 1345, 1456, 1981, 2161, 2426, 2749
MaeI	CTAG	5 cut(s)	1188, 1325, 1865, 2358, 2465
MaeII	ACGT	4 cut(s)	23, 1699, 1720, 2091
MaeIII	GTNAC	8 cut(s)	6, 133, 664, 1012, 1183, 1827, 2309, 2553
MfeI	CAATTG	1 cut(s)	2604
MflI	RGATCY	3 cut(s)	449, 571, 1327
MhlI	GDGCHC	3 cut(s)	765, 1805, 1878
MlsI	TGGCCA	2 cut(s)	318, 1167
MluNI	TGGCCA	2 cut(s)	318, 1167
MlyI	GAGTC	2 cut(s)	680, 2870
MmeI	TCCRAC	4 cut(s)	84, 283, 2447, 2511
Mox20I	TGGCCA	2 cut(s)	318, 1167
Mph1103I	ATGCAT	4 cut(s)	405, 1360, 2375, 2441
MroXI	GAANNNNTTC	2 cut(s)	1518, 2726
MscI	TGGCCA	2 cut(s)	318, 1167
MseI	TTAA	9 cut(s)	357, 483, 567, 750, 900, 1293, 1535, 2649, 2753
MslI	CAYNNNNRTG	3 cut(s)	950, 2595, 2747
Msp20I	TGGCCA	2 cut(s)	318, 1167
MspA1I	CMGCKG	1 cut(s)	2006
MspI	CCGG	7 cut(s)	97, 156, 618, 1411, 1879, 2562, 3003
MspR9I	CCNGG	3 cut(s)	98, 717, 3032
MunI	CAATTG	1 cut(s)	2604
Mva1269I	GAATGC	2 cut(s)	1509, 2439
MvaI	CCWGG	2 cut(s)	717, 3032
MvnI	CGCG	2 cut(s)	132, 1512
NciI	CCSGG	1 cut(s)	98
NcoI	CCATGG	1 cut(s)	2636
NdeI	CATATG	2 cut(s)	2596, 2950
NlaIII	CATG	9 cut(s)	407, 446, 949, 1050, 1669, 1841, 1956, 2274, 2640
NlaIV	GGNNCC	4 cut(s)	872, 1224, 1409, 2340
NmeAIII	GCCGAG	1 cut(s)	255
NmuCI	GTSAC	6 cut(s)	6, 133, 664, 1827, 2309, 2553
NsiI	ATGCAT	4 cut(s)	405, 1360, 2375, 2441
NspI	RCATGY	3 cut(s)	407, 1841, 1956
NspV	TTCGAA	1 cut(s)	2975
PaeI	GCATGC	1 cut(s)	407
PagI	TCATGA	1 cut(s)	945
PciI	ACATGT	1 cut(s)	1837
PciSI	GCTCTTC	1 cut(s)	815
PcsI	WCGNNNNNNNCGW	2 cut(s)	50, 1726
PctI	GAATGC	2 cut(s)	1509, 2439
PdmI	GAANNNNTTC	2 cut(s)	1518, 2726
PfeI	GAWTC	9 cut(s)	458, 697, 1004, 1043, 1280, 1868, 2632, 2692, 2710
PflFI	GACNNNGTC	1 cut(s)	25
PflMI	CCANNNNNTGG	2 cut(s)	781, 2384
PfoI	TCCNGGA	2 cut(s)	96, 3030
PinAI	ACCGGT	3 cut(s)	155, 1878, 2561
PleI	GAGTC	2 cut(s)	680, 2869
PpsI	GAGTC	2 cut(s)	680, 2869
PscI	ACATGT	1 cut(s)	1837
PshAI	GACNNNNGTC	1 cut(s)	2564
PshBI	ATTAAT	2 cut(s)	1293, 1535
Psp6I	CCWGG	2 cut(s)	715, 3030
PspEI	GGTNACC	1 cut(s)	664
PspGI	CCWGG	2 cut(s)	715, 3030
PspN4I	GGNNCC	4 cut(s)	872, 1224, 1409, 2340
PspPI	GGNCC	4 cut(s)	1223, 1408, 1887, 2339
PstI	CTGCAG	2 cut(s)	2219, 2589
PstNI	CAGNNNCTG	1 cut(s)	493
PsuI	RGATCY	3 cut(s)	449, 571, 1327
PsyI	GACNNNGTC	1 cut(s)	25
PvuII	CAGCTG	1 cut(s)	2006
RsaI	GTAC	5 cut(s)	1482, 1773, 2055, 2768, 2784
RsaNI	GTAC	5 cut(s)	1481, 1772, 2054, 2767, 2783
RseI	CAYNNNNRTG	3 cut(s)	950, 2595, 2747
SapI	GCTCTTC	1 cut(s)	815
SaqAI	TTAA	9 cut(s)	357, 483, 567, 750, 900, 1293, 1535, 2649, 2753
Sau96I	GGNCC	4 cut(s)	1223, 1408, 1887, 2339
SchI	GAGTC	2 cut(s)	680, 2870
ScrFI	CCNGG	3 cut(s)	98, 717, 3032
SduI	GDGCHC	3 cut(s)	765, 1805, 1878
SfaNI	GCATC	7 cut(s)	390, 1345, 1456, 1981, 2161, 2426, 2749
SfcI	CTRYAG	6 cut(s)	1986, 2215, 2411, 2585, 2683, 2816
SfuI	TTCGAA	1 cut(s)	2975
SinI	GGWCC	2 cut(s)	1223, 2339
SmiMI	CAYNNNNRTG	3 cut(s)	950, 2595, 2747
SmlI	CTYRAG	4 cut(s)	511, 738, 1484, 2018
SmoI	CTYRAG	4 cut(s)	511, 738, 1484, 2018
SphI	GCATGC	1 cut(s)	407
SsiI	CCGC	3 cut(s)	162, 2719, 3012
SspI	AATATT	3 cut(s)	710, 997, 1533
SspMI	CTAG	5 cut(s)	1188, 1325, 1865, 2358, 2465
StyD4I	CCNGG	3 cut(s)	96, 715, 3030
StyI	CCWWGG	3 cut(s)	338, 1023, 2636
TaaI	ACNGT	5 cut(s)	1076, 1771, 2415, 2509, 2687
TaiI	ACGT	4 cut(s)	26, 1702, 1723, 2094
TaqII	GACCGA	1 cut(s)	19
TatI	WGTACW	3 cut(s)	1480, 2766, 2782
TfiI	GAWTC	9 cut(s)	458, 697, 1004, 1043, 1280, 1868, 2632, 2692, 2710
Tru1I	TTAA	9 cut(s)	357, 483, 567, 750, 900, 1293, 1535, 2649, 2753
Tru9I	TTAA	9 cut(s)	357, 483, 567, 750, 900, 1293, 1535, 2649, 2753
TscAI	CASTG	6 cut(s)	861, 937, 1392, 1878, 2562, 2692
TseFI	GTSAC	6 cut(s)	6, 133, 664, 1827, 2309, 2553
Tsp45I	GTSAC	6 cut(s)	6, 133, 664, 1827, 2309, 2553
TspGWI	ACGGA	1 cut(s)	1215
TspRI	CASTG	6 cut(s)	861, 937, 1392, 1878, 2562, 2692
Tth111I	GACNNNGTC	1 cut(s)	25
Van91I	CCANNNNNTGG	2 cut(s)	781, 2384
VneI	GTGCAC	2 cut(s)	761, 1874
VpaK11BI	GGWCC	2 cut(s)	1223, 2339
VspI	ATTAAT	2 cut(s)	1293, 1535
XbaI	TCTAGA	2 cut(s)	1324, 2464
XceI	RCATGY	3 cut(s)	407, 1841, 1956
XmiI	GTMKAC	2 cut(s)	1901, 2138
XmnI	GAANNNNTTC	2 cut(s)	1518, 2726
XspI	CTAG	5 cut(s)	1188, 1325, 1865, 2358, 2465
ZraI	GACGTC	1 cut(s)	24
Zsp2I	ATGCAT	4 cut(s)	405, 1360, 2375, 2441

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.