Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0015967

Physical Location & Seq

Genomic Coordinates Reverse (-)

13693558 .. 13699992

Gene Structure 6435 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000011913

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3081 bp

ATGGCGTCTCCGACGTCACCAACGTCGTCGTGCAGCTCTGCGCACTCGTCGACATTTGCGTCGTCCAAATTCTCGGACCTTCCGGTGATGGCGCTCCGGGAGAAAATCCTCGAGAAAATCCTCGAGAATCGTGTCACTCTCATCGTCGGCGAGACCGGTTGCGGGAAAAGCTCGCAAATACCGCAGTTTCTACTGGAAGCGAATGTGAAGCCAATTCTATGTACACAGCCTAGGAGGTTTGCGGTAGTAGCTGTTGCGAAAATGGTTGCACAAGCTCGTAATAGTGAGCTTGGTGGAGAGGTTGGATATCACATAGGCCACTCAAAGCACTTGTCACCAAGATCAGCGATTGTTTTTAAAACTGCTGGAGTTTTACTGGATGAAATGCGGGACAAGGGGATGCATGCACTTGATTACAAGGTCATTGTTCTCGATGAAGTGCATGAAAGATCTGTGGAGTCTGATCTTGTTCTTGTTTGTGTGAAGCAGTTTATGATGAAGAACAATAACTTGAGGGTGGTGTTGATGTCTGCAACTGCTGATATTAATAGGTACAGGGATTACTTTAAGGATCTTGGCAGGGATGAACGAGTGGAAGTGCTTGCAATCCCTAACTCTGGCCAGAAAACCATTTTTCAGAAAAGGGTTTCTTATCTTGAAGAGGTGACTGATCTTCTCAACATCGATTCGGAGTCGCTTTCTTTGACATATTGTTCAGGGCCACGTCCTTCATTGGCCAAAGCTGAGATTAAGCCTGAAGTGCACAATATTATTCATCAATTGGTGTTGCATATTCACGAGCAAGAGCCCGACATTGAAAAGAGCATTTTGATTTTTCTTCCAACATACTATGCCCTGGAGCAGCAATGGTTCCTTCTGAAGCCTCTTAGTTCATCTTTTAAAGTTCACATTTTACATAGTAGCATTGATACTGAACAAGCTCTCATGACCATGAAGATCTGGAAATCCCATCGTAAAGTGATACTGGCCACAAACATTGCTGAATCATCAGTAACAATTCCCAAGGTGGCTTACGTTATTGATTCATGCCGATCTTTACAAGTCTTCTGGCACGCTTATGAGAAAAGGGAATGTGCAAGCCTTGTTTGGGTTTCTAAGTCTCAGGCTAATCAGCGTAGAGGGAGAACTGGTCGAACATGTGATGGCCAGATTTATCGGTTGGTTACTCGATCATTTTACGGCCAGCTTGAGGATTTCGAAGGTCCCTCTATACTGAGGTTGTCATTGAGGATGCAAGTGCTTCACATTTGCTGCTCTGATTCCAAAGCCATTAATGATCCCAAGGCCTTGTTGCAGAAGGCCTTAGATCCACCACCTCCTGATGTTGTTGAAGAAGCGTTGAATTTGCTGGTTCATATGCAAGCATTGGAAAGAACACCTCCAAGGGGTCGGTATGAGCCAACATTTTATGGGCGATTGCTGGCCAGTTTCTCATTGTCTTTCGATGCCTCTGTGCTTGTACTTAAGTTTGGAGATGTTGGAATGCTGCGTGAAGGCATTCTTCTGGGCATATTAATGGATACACAACCTCTGCCTATTCTTCGTCCTTTTGGAGATGAACTTCTGTGTTCACAATATGCTGATAGCTACTTTAGTGGAGATGATTACAGTACTGGCCTAACGGGAAAGAAGGAAACAGCATTTATGGGAAACTTGTGCGCATTTCAATTTTGGCAACGTGTTTTCAAGGATAAACACCGTGTTGAGAATTTGAAGCAACTTCTTCAGTTTGATGTGATGAAAGCTACTATATTGGAACTTCCCAAGATGGAAGACTGGTGCTCTTTCCATAATCTTGTGCACTCATCACTGAATCATGTCTCTGAAATATATGAAGATATTCTTCATTCAGTGCACCGGTTTCGCCCCAAGTTTTTGTCCACATCTAATGGCTTACCATCCTACTATGATCCGTATGAATATGAGCATACATGTATTCTTACATGTCAACAACCAAATGGAGATGCAGATGCTCTTGCTACGGACGATAGGCATCTTGAGCCATCTGGTGAAACAACAAAATGTTTTGCTGTACCTTTTGTTGCTTCAGATCACTTCCGACAGAATGATGTGGCTAGAAAGTTGGCAATGATAGTGAAACAGATAAGAGTACCGAATACCGAAGATGCATCGAGTAATCAGGGTTTAAATGTTGGTGACGGTTATCATGTCGATGGGGACGCTCCTGTTTGTATTTATTTTATCAATGGGTCCTGCAAATGGGGCAGTGAATGCAGGTTTTCTCATTCACTCAAAGGACAGAGAACTCCGTGCAAATTTTTCTTTACTCTAAAGGGTTGTCGAAACGGAGAGTCATGCTTGTTTTCTCATAATGAGAGTCCATTACTAACATCATCCAGCTCATCTAGCTTATCCAGCTCAAACTTTTGCCTGCCAGAAGATGGCAAAGCTACAGCTCTGTCCCTATTAAATCTGTTTTCCTTTTCTTCAGATGGATACCTTCTTTTACTGGATGACACCAATCTACATTTTACCTCAAAGTTAGCTAGCTTTTATGAACCATCCAAAATAATCTCCACAACATGTTTGTCAGACACATCCATATTTGACCCTTCGTTGACGGGTATCCAAATTTTGTGGGGGCTTCATCACCCATATCAGACCATCATTTCCAACGGAGTGAACCAGATCCCCTGGAAAGAAGTCAAGTACGTGCTCTGGTTTCCTAATTTTGAAAGCTACAGTGAGAATTTGGAAATACAGAAAACTCTCTTGCAGAGTTTCTTCGAGTATCTTGCCATCCGAATGTTGGGTGATGCCTTGGAAATGCGAGTCATTCTCACCCTGAACAATATCAGGTTTTCACAGCTGCAGGCGGAGAAGTTTGGTAGGGATAGCTTTTTCTTCCTTACAGAGTCATTCCCATTTGATGAGAACAGCTTCGGAGAGTTGCCAGACAAGGTCAGCACAAAGAAGCCAATGATGGCATCAAGGCCAACTTCCTATGTTTTCGACCTACACCCACCTTCAGACTCTCAGTTTGGTAACTATGCAGCCGTGCTTCAAAAATCTCTTCGCGATGTCCAGAAACATGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C zf-CCCH

Protein Analysis

1027

Amino Acids

115.87

Weight (kDa)

6.38

Isoelectric Point (pI)

44.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	34 - 181	4e-08	DEAD/DEAH box helicase
Helicase_C	PF00271	269 - 386	7.4e-13	Helicase conserved C-terminal domain
zf-CCCH_4	PF18044	736 - 756	2.2e-06	CCCH-type zinc finger
zf_CCCH_4	PF18345	738 - 756	5.3e-06	Zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0015967)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47680
fragaria_vesca	FvH4_2g25691
malus_domestica	MD03G1287700.v1.1
prunus_persica	Prupe.8G260900_v2.0.a1
pyrus_communis	pycom03g22780
rosa_chinensis	RchiOBHm_Chr6g0293951
rosa_laevigata	RLG00000011913
rosa_multiflora	Rmu_sc0005647.1_g000006
rosa_roxburghii	Rroxscaffold_7G00173800
rosa_rugosa	Rorug06G0239500
rosa_samantha	Rh6AG352600 Rh6BG359500 Rh6CG366800 Rh6DG353200
rosa_wichuraiana	Rw6G030710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	58
AatII	GACGTC	1 cut(s)	17
Acc16I	TGCGCA	2 cut(s)	42, 1681
Acc36I	ACCTGC	1 cut(s)	2247
AccB7I	CCANNNNNTGG	1 cut(s)	2423
AccI	GTMKAC	1 cut(s)	50
AccII	CGCG	1 cut(s)	3060
AciI	CCGC	5 cut(s)	162, 182, 242, 388, 2858
AclWI	GGATC	5 cut(s)	579, 1292, 1322, 1925, 2665
AcoI	YGGCCR	6 cut(s)	619, 735, 987, 1165, 1201, 1443
AcsI	RAATTY	6 cut(s)	68, 1363, 1729, 2297, 2613, 2731
AcuI	CTGAAG	6 cut(s)	777, 899, 1730, 2052, 2454, 2994
AcyI	GRCGYC	2 cut(s)	5, 14
AfaI	GTAC	7 cut(s)	223, 554, 1482, 1633, 2055, 2133, 2693
AfiI	CCNNNNNNNGG	8 cut(s)	162, 617, 1108, 1210, 1406, 2241, 2423, 2791
AflII	CTTAAG	1 cut(s)	1484
AflIII	ACRYGT	6 cut(s)	1157, 1699, 1952, 1964, 2564, 3073
AgeI	ACCGGT	2 cut(s)	155, 1878
AgsI	TTSAA	9 cut(s)	659, 818, 1352, 1363, 1688, 1708, 1735, 2717, 3047
AjiI	CACGTC	1 cut(s)	725
AjnI	CCWGG	2 cut(s)	855, 2675
Alw21I	GWGCWC	5 cut(s)	765, 1805, 1824, 1878, 2700
Alw26I	GTCTC	4 cut(s)	12, 146, 1125, 1846
Alw44I	GTGCAC	3 cut(s)	761, 1820, 1874
AlwI	GGATC	5 cut(s)	579, 1292, 1322, 1925, 2665
Ama87I	CYCGRG	2 cut(s)	110, 122
ApaLI	GTGCAC	3 cut(s)	761, 1820, 1874
ApeKI	GCWGC	6 cut(s)	33, 862, 1272, 1507, 2851, 3035
ApoI	RAATTY	6 cut(s)	68, 1363, 1729, 2297, 2613, 2731
ArsI	GACNNNNNNTTYG	4 cut(s)	317, 349, 3005, 3037
AseI	ATTAAT	3 cut(s)	546, 1293, 1535
AsiGI	ACCGGT	2 cut(s)	155, 1878
Asp700I	GAANNNNTTC	3 cut(s)	1518, 1860, 2921
AspA2I	CCTAGG	1 cut(s)	230
AspLEI	GCGC	3 cut(s)	43, 94, 1682
AspS9I	GGNCC	4 cut(s)	76, 719, 1223, 2232
AsuC2I	CCSGG	1 cut(s)	98
AsuHPI	GGTGA	9 cut(s)	9, 97, 327, 676, 2042, 2189, 2624, 2807, 2815
AsuII	TTCGAA	1 cut(s)	1218
AsuNHI	GCTAGC	1 cut(s)	2528
AvaI	CYCGRG	2 cut(s)	110, 122
AvaII	GGWCC	3 cut(s)	76, 1223, 2232
AvrII	CCTAGG	1 cut(s)	230
BaeGI	GKGCMC	3 cut(s)	765, 1824, 1878
BalI	TGGCCA	5 cut(s)	621, 737, 989, 1167, 1445
BanII	GRGCYC	1 cut(s)	810
BauI	CACGAG	1 cut(s)	797
BbsI	GAAGAC	2 cut(s)	1057, 1800
Bbv12I	GWGCWC	5 cut(s)	765, 1805, 1824, 1878, 2700
BbvI	GCAGC	6 cut(s)	45, 874, 1259, 1494, 2838, 3047
BceAI	ACGGC	2 cut(s)	1216, 3023
BcgI	CGANNNNNNTGC	4 cut(s)	1865, 1899, 2759, 2793
BciT130I	CCWGG	2 cut(s)	857, 2677
BciVI	GTATCC	3 cut(s)	1534, 2471, 2618
BcnI	CCSGG	1 cut(s)	98
BcoDI	GTCTC	4 cut(s)	12, 146, 1125, 1846
BfaI	CTAG	4 cut(s)	231, 2097, 2388, 2529
BfmI	CTRYAG	3 cut(s)	2433, 2722, 2852
BfoI	RGCGCY	1 cut(s)	95
BfrI	CTTAAG	1 cut(s)	1484
BfuAI	ACCTGC	1 cut(s)	2247
BfuI	GTATCC	3 cut(s)	1534, 2471, 2618
BglII	AGATCT	2 cut(s)	449, 957
BisI	GCNGC	6 cut(s)	34, 863, 1273, 1508, 2852, 3036
BlnI	CCTAGG	1 cut(s)	230
BlsI	GCNGC	6 cut(s)	35, 864, 1274, 1509, 2853, 3037
BmcAI	AGTACT	1 cut(s)	1633
Bme1390I	CCNGG	3 cut(s)	98, 857, 2677
Bme18I	GGWCC	3 cut(s)	76, 1223, 2232
BmeT110I	CYCGRG	2 cut(s)	110, 122
BmgBI	CACGTC	1 cut(s)	725
BmgT120I	GGNCC	4 cut(s)	76, 719, 1223, 2232
BmiI	GGNNCC	3 cut(s)	872, 1225, 2233
BmrFI	CCNGG	3 cut(s)	98, 857, 2677
BmtI	GCTAGC	1 cut(s)	2532
BpiI	GAAGAC	2 cut(s)	1057, 1800
BplI	GAGNNNNNCTC	2 cut(s)	2805, 2837
BpmI	CTGGAG	2 cut(s)	387, 878
Bpu14I	TTCGAA	1 cut(s)	1218
BpuEI	CTTGAG	3 cut(s)	532, 1229, 2039
BpuMI	CCSGG	1 cut(s)	98
Bsa29I	ATCGAT	1 cut(s)	684
BsaAI	YACGTR	1 cut(s)	2695
BsaBI	GATNNNNATC	1 cut(s)	2013
BsaHI	GRCGYC	2 cut(s)	5, 14
BsaI	GGTCTC	1 cut(s)	146
BsaJI	CCNNGG	7 cut(s)	230, 855, 1023, 1302, 1403, 2675, 2802
BsaWI	WCCGGW	3 cut(s)	82, 155, 1878
BsaXI	ACNNNNNCTCC	2 cut(s)	1135, 1165
Bsc4I	CCNNNNNNNGG	8 cut(s)	162, 617, 1108, 1210, 1406, 2241, 2423, 2791
Bse118I	RCCGGY	2 cut(s)	155, 1878
Bse1I	ACTGG	8 cut(s)	198, 381, 990, 1153, 1446, 1639, 1802, 2496
Bse3DI	GCAATG	3 cut(s)	872, 996, 2115
Bse8I	GATNNNNATC	1 cut(s)	2013
BseBI	CCWGG	2 cut(s)	857, 2677
BseCI	ATCGAT	1 cut(s)	684
BseDI	CCNNGG	7 cut(s)	230, 855, 1023, 1302, 1403, 2675, 2802
BseJI	GATNNNNATC	1 cut(s)	2013
BseLI	CCNNNNNNNGG	8 cut(s)	162, 617, 1108, 1210, 1406, 2241, 2423, 2791
BseMI	GCAATG	3 cut(s)	872, 996, 2115
BseMII	CTCAG	4 cut(s)	735, 1136, 1226, 3032
BseNI	ACTGG	8 cut(s)	198, 381, 990, 1153, 1446, 1639, 1802, 2496
BseSI	GKGCMC	3 cut(s)	765, 1824, 1878
BseXI	GCAGC	6 cut(s)	45, 874, 1259, 1494, 2838, 3047
BsgI	GTGCAG	1 cut(s)	52
Bsh1236I	CGCG	1 cut(s)	3060
BshTI	ACCGGT	2 cut(s)	155, 1878
BshVI	ATCGAT	1 cut(s)	684
BsiHKAI	GWGCWC	5 cut(s)	765, 1805, 1824, 1878, 2700
BsiHKCI	CYCGRG	2 cut(s)	110, 122
BsiSI	CCGG	4 cut(s)	83, 97, 156, 1879
BslFI	GGGAC	4 cut(s)	404, 1209, 2213, 2428
BslI	CCNNNNNNNGG	8 cut(s)	162, 617, 1108, 1210, 1406, 2241, 2423, 2791
BsmAI	GTCTC	4 cut(s)	12, 146, 1125, 1846
BsmBI	CGTCTC	1 cut(s)	12
BsmFI	GGGAC	4 cut(s)	404, 1209, 2213, 2428
BsmI	GAATGC	3 cut(s)	1509, 1518, 2258
Bso31I	GGTCTC	1 cut(s)	146
BsoBI	CYCGRG	2 cut(s)	110, 122
Bsp119I	TTCGAA	1 cut(s)	1218
Bsp1286I	GDGCHC	6 cut(s)	765, 810, 1805, 1824, 1878, 2700
Bsp1407I	TGTACA	1 cut(s)	221
Bsp68I	TCGCGA	1 cut(s)	3060
BspACI	CCGC	5 cut(s)	162, 182, 242, 388, 2858
BspCNI	CTCAG	4 cut(s)	736, 1135, 1227, 3031
BspDI	ATCGAT	1 cut(s)	684
BspFNI	CGCG	1 cut(s)	3060
BspHI	TCATGA	1 cut(s)	945
BspLI	GGNNCC	3 cut(s)	872, 1225, 2233
BspMAI	CTGCAG	1 cut(s)	2856
BspMI	ACCTGC	1 cut(s)	2247
BspOI	GCTAGC	1 cut(s)	2532
BspPI	GGATC	5 cut(s)	579, 1292, 1322, 1925, 2665
BspT104I	TTCGAA	1 cut(s)	1218
BspTI	CTTAAG	1 cut(s)	1484
BspTNI	GGTCTC	1 cut(s)	146
BsrDI	GCAATG	3 cut(s)	872, 996, 2115
BsrFI	RCCGGY	2 cut(s)	155, 1878
BsrGI	TGTACA	1 cut(s)	221
BsrI	ACTGG	8 cut(s)	198, 381, 990, 1153, 1446, 1639, 1802, 2496
BssAI	RCCGGY	2 cut(s)	155, 1878
BssECI	CCNNGG	7 cut(s)	230, 855, 1023, 1302, 1403, 2675, 2802
BssNI	GRCGYC	2 cut(s)	5, 14
BssSI	CACGAG	1 cut(s)	797
BssT1I	CCWWGG	5 cut(s)	230, 1023, 1302, 1403, 2802
Bst2BI	CACGAG	1 cut(s)	797
Bst2UI	CCWGG	2 cut(s)	857, 2677
Bst4CI	ACNGT	4 cut(s)	1631, 1721, 2183, 2726
Bst6I	CTCTTC	2 cut(s)	654, 3060
BstACI	GRCGYC	2 cut(s)	5, 14
BstAFI	CTTAAG	1 cut(s)	1484
BstAPI	GCANNNNNTGC	1 cut(s)	2253
BstAUI	TGTACA	1 cut(s)	221
BstBAI	YACGTR	1 cut(s)	2695
BstBI	TTCGAA	1 cut(s)	1218
BstDEI	CTNAG	7 cut(s)	744, 887, 1116, 1122, 1235, 1324, 3018
BstFNI	CGCG	1 cut(s)	3060
BstH2I	RGCGCY	1 cut(s)	95
BstHHI	GCGC	3 cut(s)	43, 94, 1682
BstMAI	GTCTC	4 cut(s)	12, 146, 1125, 1846
BstMWI	GCNNNNNNNGC	9 cut(s)	168, 181, 248, 760, 2020, 2244, 2253, 2388, 2397
BstNI	CCWGG	2 cut(s)	857, 2677
BstNSI	RCATGY	6 cut(s)	407, 1161, 1956, 1968, 2568, 3077
BstSCI	CCNGG	3 cut(s)	96, 855, 2675
BstSFI	CTRYAG	3 cut(s)	2433, 2722, 2852
BstSLI	GKGCMC	3 cut(s)	765, 1824, 1878
BstUI	CGCG	1 cut(s)	3060
BstV1I	GCAGC	6 cut(s)	45, 874, 1259, 1494, 2838, 3047
BstV2I	GAAGAC	2 cut(s)	1057, 1800
BstX2I	RGATCY	5 cut(s)	449, 571, 957, 1327, 2670
BstYI	RGATCY	5 cut(s)	449, 571, 957, 1327, 2670
Bsu15I	ATCGAT	1 cut(s)	684
BsuI	GTATCC	3 cut(s)	1534, 2471, 2618
BsuTUI	ATCGAT	1 cut(s)	684
BtgZI	GCGATG	1 cut(s)	3075
BtrI	CACGTC	1 cut(s)	725
BtsI	GCAGTG	1 cut(s)	2254
BtsIMutI	CAGTG	4 cut(s)	1829, 1878, 2254, 2731
BtuMI	TCGCGA	1 cut(s)	3060
BveI	ACCTGC	1 cut(s)	2247
CciI	TCATGA	1 cut(s)	945
CfoI	GCGC	3 cut(s)	43, 94, 1682
Cfr10I	RCCGGY	2 cut(s)	155, 1878
Cfr13I	GGNCC	4 cut(s)	76, 719, 1223, 2232
ClaI	ATCGAT	1 cut(s)	684
CseI	GACGC	2 cut(s)	48, 2210
Csp6I	GTAC	7 cut(s)	222, 553, 1481, 1632, 2054, 2132, 2692
CspAI	ACCGGT	2 cut(s)	155, 1878
CspCI	CAANNNNNGTGG	4 cut(s)	979, 1014, 2600, 2635
CviQI	GTAC	7 cut(s)	222, 553, 1481, 1632, 2054, 2132, 2692
DdeI	CTNAG	7 cut(s)	744, 887, 1116, 1122, 1235, 1324, 3018
DraI	TTTAAA	3 cut(s)	358, 901, 2169
DrdI	GACNNNNNNGTC	1 cut(s)	58
DseDI	GACNNNNNNGTC	1 cut(s)	58
EaeI	YGGCCR	6 cut(s)	619, 735, 987, 1165, 1201, 1443
Eam1104I	CTCTTC	2 cut(s)	654, 3060
EarI	CTCTTC	2 cut(s)	654, 3060
EciI	GGCGGA	1 cut(s)	2873
Eco130I	CCWWGG	5 cut(s)	230, 1023, 1302, 1403, 2802
Eco147I	AGGCCT	2 cut(s)	1307, 1322
Eco24I	GRGCYC	1 cut(s)	810
Eco31I	GGTCTC	1 cut(s)	146
Eco32I	GATATC	1 cut(s)	308
Eco47I	GGWCC	3 cut(s)	76, 1223, 2232
Eco57I	CTGAAG	6 cut(s)	777, 899, 1730, 2052, 2454, 2994
Eco88I	CYCGRG	2 cut(s)	110, 122
EcoO109I	RGGNCCY	2 cut(s)	1223, 2232
EcoRII	CCWGG	2 cut(s)	855, 2675
EcoRV	GATATC	1 cut(s)	308
EcoT14I	CCWWGG	5 cut(s)	230, 1023, 1302, 1403, 2802
EcoT22I	ATGCAT	2 cut(s)	405, 2152
EcoT38I	GRGCYC	1 cut(s)	810
ErhI	CCWWGG	5 cut(s)	230, 1023, 1302, 1403, 2802
Esp3I	CGTCTC	1 cut(s)	12
FalI	AAGNNNNNCTT	8 cut(s)	634, 666, 1506, 1538, 1848, 1880, 2965, 2997
FaqI	GGGAC	4 cut(s)	404, 1209, 2213, 2428
FauI	CCCGC	2 cut(s)	155, 381
FauNDI	CATATG	1 cut(s)	1377
FblI	GTMKAC	1 cut(s)	50
Fnu4HI	GCNGC	6 cut(s)	34, 863, 1273, 1508, 2852, 3036
FriOI	GRGCYC	1 cut(s)	810
Fsp4HI	GCNGC	6 cut(s)	34, 863, 1273, 1508, 2852, 3036
FspAI	RTGCGCAY	1 cut(s)	1681
FspBI	CTAG	4 cut(s)	231, 2097, 2388, 2529
FspI	TGCGCA	2 cut(s)	42, 1681
GlaI	GCGC	3 cut(s)	42, 93, 1681
GluI	GCNGC	6 cut(s)	34, 863, 1273, 1508, 2852, 3036
GsuI	CTGGAG	2 cut(s)	387, 878
HaeII	RGCGCY	1 cut(s)	95
HapII	CCGG	4 cut(s)	83, 97, 156, 1879
HgaI	GACGC	2 cut(s)	48, 2210
HhaI	GCGC	3 cut(s)	43, 94, 1682
Hin1I	GRCGYC	2 cut(s)	5, 14
Hin6I	GCGC	3 cut(s)	41, 92, 1680
HinP1I	GCGC	3 cut(s)	41, 92, 1680
HincII	GTYRAC	3 cut(s)	51, 1970, 2601
HindII	GTYRAC	3 cut(s)	51, 1970, 2601
HpaII	CCGG	4 cut(s)	83, 97, 156, 1879
HphI	GGTGA	9 cut(s)	9, 97, 327, 676, 2042, 2189, 2624, 2807, 2815
Hpy99I	CGWCG	6 cut(s)	16, 28, 31, 52, 64, 149
HpyCH4III	ACNGT	4 cut(s)	1631, 1721, 2183, 2726
HpyCH4IV	ACGT	6 cut(s)	14, 23, 724, 1035, 1699, 2694
HpyF10VI	GCNNNNNNNGC	9 cut(s)	168, 181, 248, 760, 2020, 2244, 2253, 2388, 2397
HpyF3I	CTNAG	7 cut(s)	744, 887, 1116, 1122, 1235, 1324, 3018
HpySE526I	ACGT	6 cut(s)	14, 23, 724, 1035, 1699, 2694
Hsp92I	GRCGYC	2 cut(s)	5, 14
HspAI	GCGC	3 cut(s)	41, 92, 1680
LmnI	GCTCC	3 cut(s)	99, 859, 2209
Lsp1109I	GCAGC	6 cut(s)	45, 874, 1259, 1494, 2838, 3047
MaeI	CTAG	4 cut(s)	231, 2097, 2388, 2529
MaeII	ACGT	6 cut(s)	14, 23, 724, 1035, 1699, 2694
MaeIII	GTNAC	8 cut(s)	15, 133, 333, 664, 1012, 1183, 2177, 3026
MfeI	CAATTG	1 cut(s)	779
MflI	RGATCY	5 cut(s)	449, 571, 957, 1327, 2670
MhlI	GDGCHC	6 cut(s)	765, 810, 1805, 1824, 1878, 2700
MlsI	TGGCCA	5 cut(s)	621, 737, 989, 1167, 1445
MluNI	TGGCCA	5 cut(s)	621, 737, 989, 1167, 1445
MlyI	GAGTC	7 cut(s)	467, 701, 2342, 2368, 2823, 2906, 3008
MmeI	TCCRAC	6 cut(s)	35, 283, 866, 1480, 2104, 2679
Mox20I	TGGCCA	5 cut(s)	621, 737, 989, 1167, 1445
Mph1103I	ATGCAT	2 cut(s)	405, 2152
MroXI	GAANNNNTTC	3 cut(s)	1518, 1860, 2921
MscI	TGGCCA	5 cut(s)	621, 737, 989, 1167, 1445
MslI	CAYNNNNRTG	5 cut(s)	950, 1077, 1535, 2193, 2786
Msp20I	TGGCCA	5 cut(s)	621, 737, 989, 1167, 1445
MspA1I	CMGCKG	1 cut(s)	2851
MspCI	CTTAAG	1 cut(s)	1484
MspI	CCGG	4 cut(s)	83, 97, 156, 1879
MspR9I	CCNGG	3 cut(s)	98, 857, 2677
MunI	CAATTG	1 cut(s)	779
Mva1269I	GAATGC	3 cut(s)	1509, 1518, 2258
MvaI	CCWGG	2 cut(s)	857, 2677
MvnI	CGCG	1 cut(s)	3060
MwoI	GCNNNNNNNGC	9 cut(s)	168, 181, 248, 760, 2020, 2244, 2253, 2388, 2397
NciI	CCSGG	1 cut(s)	98
NdeI	CATATG	1 cut(s)	1377
NheI	GCTAGC	1 cut(s)	2528
NlaIV	GGNNCC	3 cut(s)	872, 1225, 2233
NmuCI	GTSAC	5 cut(s)	15, 133, 333, 664, 2177
NruI	TCGCGA	1 cut(s)	3060
NsbI	TGCGCA	2 cut(s)	42, 1681
NsiI	ATGCAT	2 cut(s)	405, 2152
NspI	RCATGY	6 cut(s)	407, 1161, 1956, 1968, 2568, 3077
NspV	TTCGAA	1 cut(s)	1218
PaeI	GCATGC	1 cut(s)	407
PaeR7I	CTCGAG	2 cut(s)	110, 122
PagI	TCATGA	1 cut(s)	945
PceI	AGGCCT	2 cut(s)	1307, 1322
PciI	ACATGT	5 cut(s)	1157, 1952, 1964, 2564, 3073
PcsI	WCGNNNNNNNCGW	2 cut(s)	20, 56
PctI	GAATGC	3 cut(s)	1509, 1518, 2258
PdmI	GAANNNNTTC	3 cut(s)	1518, 1860, 2921
PfeI	GAWTC	6 cut(s)	127, 686, 1004, 1043, 1280, 1834
PflFI	GACNNNGTC	1 cut(s)	2942
PflMI	CCANNNNNTGG	1 cut(s)	2423
PfoI	TCCNGGA	1 cut(s)	96
PinAI	ACCGGT	2 cut(s)	155, 1878
PkrI	GCNGC	6 cut(s)	35, 864, 1274, 1509, 2853, 3037
PleI	GAGTC	7 cut(s)	466, 700, 2341, 2367, 2822, 2905, 3008
PpsI	GAGTC	7 cut(s)	466, 700, 2341, 2367, 2822, 2905, 3008
Ppu21I	YACGTR	1 cut(s)	2695
PpuMI	RGGWCCY	2 cut(s)	1223, 2232
PscI	ACATGT	5 cut(s)	1157, 1952, 1964, 2564, 3073
PshBI	ATTAAT	3 cut(s)	546, 1293, 1535
Psp5II	RGGWCCY	2 cut(s)	1223, 2232
Psp6I	CCWGG	2 cut(s)	855, 2675
PspGI	CCWGG	2 cut(s)	855, 2675
PspN4I	GGNNCC	3 cut(s)	872, 1225, 2233
PspPI	GGNCC	4 cut(s)	76, 719, 1223, 2232
PspPPI	RGGWCCY	2 cut(s)	1223, 2232
PstI	CTGCAG	1 cut(s)	2856
PsuI	RGATCY	5 cut(s)	449, 571, 957, 1327, 2670
PsyI	GACNNNGTC	1 cut(s)	2942
PvuII	CAGCTG	1 cut(s)	2851
RruI	TCGCGA	1 cut(s)	3060
RsaI	GTAC	7 cut(s)	223, 554, 1482, 1633, 2055, 2133, 2693
RsaNI	GTAC	7 cut(s)	222, 553, 1481, 1632, 2054, 2132, 2692
RseI	CAYNNNNRTG	5 cut(s)	950, 1077, 1535, 2193, 2786
SalI	GTCGAC	1 cut(s)	49
SatI	GCNGC	6 cut(s)	34, 863, 1273, 1508, 2852, 3036
Sau96I	GGNCC	4 cut(s)	76, 719, 1223, 2232
ScaI	AGTACT	1 cut(s)	1633
SchI	GAGTC	7 cut(s)	467, 701, 2342, 2368, 2823, 2906, 3008
ScrFI	CCNGG	3 cut(s)	98, 857, 2677
SduI	GDGCHC	6 cut(s)	765, 810, 1805, 1824, 1878, 2700
SfcI	CTRYAG	3 cut(s)	2433, 2722, 2852
Sfr274I	CTCGAG	2 cut(s)	110, 122
SfuI	TTCGAA	1 cut(s)	1218
SinI	GGWCC	3 cut(s)	76, 1223, 2232
SlaI	CTCGAG	2 cut(s)	110, 122
SmiMI	CAYNNNNRTG	5 cut(s)	950, 1077, 1535, 2193, 2786
SmlI	CTYRAG	6 cut(s)	110, 122, 511, 1208, 1484, 2018
SmoI	CTYRAG	6 cut(s)	110, 122, 511, 1208, 1484, 2018
SphI	GCATGC	1 cut(s)	407
SseBI	AGGCCT	2 cut(s)	1307, 1322
SsiI	CCGC	5 cut(s)	162, 182, 242, 388, 2858
SspI	AATATT	1 cut(s)	769
SspMI	CTAG	4 cut(s)	231, 2097, 2388, 2529
StuI	AGGCCT	2 cut(s)	1307, 1322
StyD4I	CCNGG	3 cut(s)	96, 855, 2675
StyI	CCWWGG	5 cut(s)	230, 1023, 1302, 1403, 2802
TaaI	ACNGT	4 cut(s)	1631, 1721, 2183, 2726
TaiI	ACGT	6 cut(s)	17, 26, 727, 1038, 1702, 2697
TatI	WGTACW	3 cut(s)	221, 1480, 1631
TfiI	GAWTC	6 cut(s)	127, 686, 1004, 1043, 1280, 1834
TscAI	CASTG	4 cut(s)	1836, 1878, 2254, 2731
TseFI	GTSAC	5 cut(s)	15, 133, 333, 664, 2177
TseI	GCWGC	6 cut(s)	33, 862, 1272, 1507, 2851, 3035
Tsp45I	GTSAC	5 cut(s)	15, 133, 333, 664, 2177
TspGWI	ACGGA	5 cut(s)	1923, 2018, 2280, 2343, 2673
TspRI	CASTG	4 cut(s)	1836, 1878, 2254, 2731
Tth111I	GACNNNGTC	1 cut(s)	2942
Van91I	CCANNNNNTGG	1 cut(s)	2423
Vha464I	CTTAAG	1 cut(s)	1484
VneI	GTGCAC	3 cut(s)	761, 1820, 1874
VpaK11BI	GGWCC	3 cut(s)	76, 1223, 2232
VspI	ATTAAT	3 cut(s)	546, 1293, 1535
XapI	RAATTY	6 cut(s)	68, 1363, 1729, 2297, 2613, 2731
XceI	RCATGY	6 cut(s)	407, 1161, 1956, 1968, 2568, 3077
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2788
XhoI	CTCGAG	2 cut(s)	110, 122
XmaJI	CCTAGG	1 cut(s)	230
XmiI	GTMKAC	1 cut(s)	50
XmnI	GAANNNNTTC	3 cut(s)	1518, 1860, 2921
XspI	CTAG	4 cut(s)	231, 2097, 2388, 2529
ZraI	GACGTC	1 cut(s)	15
ZrmI	AGTACT	1 cut(s)	1633
Zsp2I	ATGCAT	2 cut(s)	405, 2152

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.