MD03G1297600.v1.1

Heparan-alpha-glucosaminide N-acetyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005188
Physical Location & Seq
Reverse (-)
37334745 .. 37338570
3826 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1297600.v1.1.491

Sequence Viewer

Length: 1452 bp
ATGTCTGCAGCCGTCGACGACGTTGCTCCTCTTCTTCATCTCTACAGAGACAGAGACACAGACAGAGACGGAGAATCATCCATTTCTAATGAGATCCCAAGTCTTGTGCCCGCCTCACCATCCAACGGCACAGATGCATCGACGCCTAATCAACGCCTGGCCTCTCTCGACGTCTTCCGTGGCCTCACCGTCGCGTTGATGATTTTGGTCGATGATGCCGGAGGAGCTTTCCCGTCGATAAACCACTCTCCGTGGTTGGGCGTTACACTTGCAGATTTTGTAATGCCATTTTTTCTTTTTCTTGTTGGCGTCTCCATCAGTCTTGCATTCAAGAAAATACCCAACAAGACAACTGCCACAAAGAAAGTCATTCTTAGAGCAATGAATCTCTTTCTCTTGGGGTTGTTACTGCAAGGTGGGTATTTCCATGGGCGTGACCATTTAACATATGGGGTTGATGTGGGCAAGATACGTTGGCTAGGTGTACTACAGAGGATTTCAATTGGATATTTGCTGGCTTCAATCTCAGAGATCTGGTTTGTTAACAATATCATGGTTGAATCACTGGTCGATTTTGCTAGGAAATACTACAATCAGTGGCTGTTTGCTATTGGATTATGCTTTTTTCACATGTGCTTTCTGTATGGTCTTTTCGTTCCAACTTGGGAATATGAAGCTGCAAGCTTGAATTTGTCTCGTTCTGGACCTGGTACTCAAATTGTTTATTGTGGAGTGAGGGGTAGTCTTGAACCTCCTTGCAATGCTGTTGGTTATATCGATCGAGTTATTCTGGGCGAGCATCACCTTTATCAACATCCTGTTTACAGAAGAACAAAGGAATGCAGTGTTAATTCTCCTGACTATGGACCTCTGCCCTCTGATTCTCCTCAATGGTGCTTTGCACCATTTGACTCAGAGGGAATCTTAAGTTCTCTGATGGCTGCTGTTACATGTTTTGTGGGATTGCATTTTGGGCACATACTATTGCATTTCAAGGACCAGAAGCAGAGGATATTGTTATGGTCTATGTCCTCCTTTCCTCTTCTAATATTTGGATATGCCTTGAAGATACTTGGTATTCCTTTCTGTAAACCACTATACACAATGAGCTATACATGTATTACAGCAGGAGCATCAGGCTTGATATTATGCATTATCTTCTACATAGTTGATGTCAAACATTTCATAAAAGTGACAGTGTTGCTGCAATGGATGGGGATGAATGCGCTCATTATATATGCTTTGGGTGCTTGTGACCTCTTCTCAGCAGGTTTGCAAGGTTTCTATTGGCGGTCACCAGAGAATAACTTGGTTGATGGGACAGAGGCATTATTGCAGGGCATGCTTCGATCGAAGAACTGGGGTACCCTAGCATTTGTAATACTTGAGATTTTATTTTGGGGTCTTGTTTCTGGGTTTCTTCACATGAAAGGCGTGTATATAAAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
DUF1624

Protein Analysis

484

Amino Acids

53.8

Weight (kDa)

7.18

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HGSNAT_cat PF07786 52 - 192 7.6e-07 Heparan-alpha-glucosaminide N-acetyltransferase, catalytic
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 174
Acc36I ACCTGC 1 cut(s) 1259
Acc65I GGTACC 1 cut(s) 1364
AccB1I GGYRCC 1 cut(s) 1364
AccI GTMKAC 1 cut(s) 15
AccII CGCG 1 cut(s) 194
AciI CCGC 2 cut(s) 111, 1291
AclWI GGATC 1 cut(s) 88
AcsI RAATTY 1 cut(s) 688
AcyI GRCGYC 3 cut(s) 143, 171, 309
AfaI GTAC 3 cut(s) 486, 712, 1366
AfiI CCNNNNNNNGG 3 cut(s) 125, 257, 863
AflII CTTAAG 1 cut(s) 925
AflIII ACRYGT 3 cut(s) 630, 950, 1115
AgsI TTSAA 8 cut(s) 331, 501, 522, 560, 688, 749, 994, 1066
AjnI CCWGG 2 cut(s) 156, 706
AluBI AGCT 4 cut(s) 227, 677, 684, 1110
AluI AGCT 4 cut(s) 227, 677, 684, 1110
Alw26I GTCTC 5 cut(s) 42, 48, 60, 316, 699
AlwI GGATC 1 cut(s) 88
AlwNI CAGNNNCTG 1 cut(s) 601
AoxI GGCC 2 cut(s) 159, 181
ApeKI GCWGC 4 cut(s) 8, 677, 941, 1204
ApoI RAATTY 1 cut(s) 688
Asp718I GGTACC 1 cut(s) 1364
AspLEI GCGC 1 cut(s) 1228
AspS9I GGNCC 3 cut(s) 704, 866, 997
AsuHPI GGTGA 4 cut(s) 108, 178, 794, 1287
AvaII GGWCC 3 cut(s) 704, 866, 997
BaeGI GKGCMC 2 cut(s) 111, 978
BaeI ACNNNNGTAYC 2 cut(s) 1348, 1381
BanI GGYRCC 1 cut(s) 1364
BbsI GAAGAC 1 cut(s) 166
BbvI GCAGC 4 cut(s) 20, 664, 928, 1191
BccI CCATC 5 cut(s) 127, 323, 931, 1207, 1310
BceAI ACGGC 1 cut(s) 142
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 2 cut(s) 158, 708
BcoDI GTCTC 5 cut(s) 42, 48, 60, 316, 699
BfaI CTAG 3 cut(s) 479, 579, 1370
BfmI CTRYAG 3 cut(s) 6, 43, 488
BfrI CTTAAG 1 cut(s) 925
BfuAI ACCTGC 1 cut(s) 1259
BglII AGATCT 1 cut(s) 531
BisI GCNGC 4 cut(s) 9, 678, 942, 1205
BlsI GCNGC 4 cut(s) 10, 679, 943, 1206
Bme1390I CCNGG 2 cut(s) 158, 708
Bme18I GGWCC 3 cut(s) 704, 866, 997
BmgT120I GGNCC 3 cut(s) 704, 866, 997
BmiI GGNNCC 1 cut(s) 1366
BmrFI CCNGG 2 cut(s) 158, 708
BmrI ACTGGG 1 cut(s) 1369
BmsI GCATC 5 cut(s) 124, 146, 205, 808, 1142
BmuI ACTGGG 1 cut(s) 1369
BpiI GAAGAC 1 cut(s) 166
BpuEI CTTGAG 1 cut(s) 1406
Bsa29I ATCGAT 1 cut(s) 777
BsaHI GRCGYC 3 cut(s) 143, 171, 309
BsaJI CCNNGG 3 cut(s) 178, 251, 427
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 257, 863
Bse1I ACTGG 2 cut(s) 570, 1364
Bse3DI GCAATG 3 cut(s) 387, 766, 1214
BseBI CCWGG 2 cut(s) 158, 708
BseCI ATCGAT 1 cut(s) 777
BseDI CCNNGG 3 cut(s) 178, 251, 427
BseGI GGATG 5 cut(s) 77, 119, 814, 1218, 1224
BseLI CCNNNNNNNGG 3 cut(s) 125, 257, 863
BseMI GCAATG 3 cut(s) 387, 766, 1214
BseMII CTCAG 3 cut(s) 540, 927, 1278
BseNI ACTGG 2 cut(s) 570, 1364
BseRI GAGGAG 3 cut(s) 18, 237, 876
BseSI GKGCMC 2 cut(s) 111, 978
BseXI GCAGC 4 cut(s) 20, 664, 928, 1191
Bsh1236I CGCG 1 cut(s) 194
Bsh1285I CGRYCG 2 cut(s) 781, 1352
BshFI GGCC 2 cut(s) 161, 183
BshNI GGYRCC 1 cut(s) 1364
BshVI ATCGAT 1 cut(s) 777
BsiEI CGRYCG 2 cut(s) 781, 1352
BsiSI CCGG 1 cut(s) 219
BslFI GGGAC 1 cut(s) 1333
BslI CCNNNNNNNGG 3 cut(s) 125, 257, 863
BsmAI GTCTC 5 cut(s) 42, 48, 60, 316, 699
BsmBI CGTCTC 2 cut(s) 60, 316
BsmFI GGGAC 1 cut(s) 1333
BsmI GAATGC 3 cut(s) 326, 845, 1228
BsnI GGCC 2 cut(s) 161, 183
Bsp1286I GDGCHC 2 cut(s) 111, 978
Bsp143I GATC 4 cut(s) 93, 531, 778, 1349
Bsp19I CCATGG 1 cut(s) 427
BspACI CCGC 2 cut(s) 111, 1291
BspANI GGCC 2 cut(s) 161, 183
BspCNI CTCAG 3 cut(s) 539, 926, 1277
BspDI ATCGAT 1 cut(s) 777
BspFNI CGCG 1 cut(s) 194
BspLI GGNNCC 1 cut(s) 1366
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 1 cut(s) 1259
BspPI GGATC 1 cut(s) 88
BspT107I GGYRCC 1 cut(s) 1364
BspTI CTTAAG 1 cut(s) 925
BsrDI GCAATG 3 cut(s) 387, 766, 1214
BsrI ACTGG 2 cut(s) 570, 1364
BssECI CCNNGG 3 cut(s) 178, 251, 427
BssMI GATC 4 cut(s) 93, 531, 778, 1349
BssNI GRCGYC 3 cut(s) 143, 171, 309
BssT1I CCWWGG 1 cut(s) 427
Bst2UI CCWGG 2 cut(s) 158, 708
Bst4CI ACNGT 3 cut(s) 190, 1198, 1449
Bst6I CTCTTC 3 cut(s) 36, 1047, 1265
BstACI GRCGYC 3 cut(s) 143, 171, 309
BstAFI CTTAAG 1 cut(s) 925
BstAPI GCANNNNNTGC 1 cut(s) 1342
BstC8I GCNNGC 5 cut(s) 111, 516, 682, 797, 1343
BstDEI CTNAG 4 cut(s) 374, 526, 913, 1264
BstDSI CCRYGG 3 cut(s) 178, 251, 427
BstEII GGTNACC 1 cut(s) 1293
BstF5I GGATG 5 cut(s) 77, 119, 814, 1218, 1224
BstFNI CGCG 1 cut(s) 194
BstHHI GCGC 1 cut(s) 1228
BstKTI GATC 4 cut(s) 96, 534, 781, 1352
BstMAI GTCTC 5 cut(s) 42, 48, 60, 316, 699
BstMBI GATC 4 cut(s) 93, 531, 778, 1349
BstMCI CGRYCG 2 cut(s) 781, 1352
BstMWI GCNNNNNNNGC 4 cut(s) 224, 973, 1247, 1342
BstNI CCWGG 2 cut(s) 158, 708
BstNSI RCATGY 4 cut(s) 634, 954, 1119, 1345
BstPI GGTNACC 1 cut(s) 1293
BstSCI CCNGG 2 cut(s) 156, 706
BstSFI CTRYAG 3 cut(s) 6, 43, 488
BstSLI GKGCMC 2 cut(s) 111, 978
BstUI CGCG 1 cut(s) 194
BstV1I GCAGC 4 cut(s) 20, 664, 928, 1191
BstV2I GAAGAC 1 cut(s) 166
BstX2I RGATCY 2 cut(s) 93, 531
BstYI RGATCY 2 cut(s) 93, 531
Bsu15I ATCGAT 1 cut(s) 777
BsuRI GGCC 2 cut(s) 161, 183
BsuTUI ATCGAT 1 cut(s) 777
BtgI CCRYGG 3 cut(s) 178, 251, 427
BtsCI GGATG 5 cut(s) 77, 119, 814, 1218, 1224
BtsI GCAGTG 1 cut(s) 850
BtsIMutI CAGTG 4 cut(s) 563, 602, 850, 1203
BveI ACCTGC 1 cut(s) 1259
Cac8I GCNNGC 5 cut(s) 111, 516, 682, 797, 1343
CaiI CAGNNNCTG 1 cut(s) 601
CfoI GCGC 1 cut(s) 1228
Cfr13I GGNCC 3 cut(s) 704, 866, 997
ClaI ATCGAT 1 cut(s) 777
CseI GACGC 2 cut(s) 151, 298
CsiI ACCWGGT 1 cut(s) 706
Csp6I GTAC 3 cut(s) 485, 711, 1365
CviAII CATG 7 cut(s) 428, 553, 631, 951, 1116, 1342, 1426
CviQI GTAC 3 cut(s) 485, 711, 1365
DdeI CTNAG 4 cut(s) 374, 526, 913, 1264
DpnI GATC 4 cut(s) 95, 533, 780, 1351
DpnII GATC 4 cut(s) 93, 531, 778, 1349
Eam1104I CTCTTC 3 cut(s) 36, 1047, 1265
EarI CTCTTC 3 cut(s) 36, 1047, 1265
Eco130I CCWWGG 1 cut(s) 427
Eco47I GGWCC 3 cut(s) 704, 866, 997
Eco91I GGTNACC 1 cut(s) 1293
EcoO65I GGTNACC 1 cut(s) 1293
EcoRII CCWGG 2 cut(s) 156, 706
EcoT14I CCWWGG 1 cut(s) 427
EcoT22I ATGCAT 2 cut(s) 139, 1154
ErhI CCWWGG 1 cut(s) 427
Esp3I CGTCTC 2 cut(s) 60, 316
FaeI CATG 7 cut(s) 431, 556, 634, 954, 1119, 1345, 1429
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 1 cut(s) 1333
FatI CATG 7 cut(s) 427, 552, 630, 950, 1115, 1341, 1425
FauI CCCGC 1 cut(s) 118
FauNDI CATATG 1 cut(s) 448
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 4 cut(s) 9, 678, 942, 1205
FokI GGATG 5 cut(s) 64, 106, 801, 1225, 1231
Fsp4HI GCNGC 4 cut(s) 9, 678, 942, 1205
FspBI CTAG 3 cut(s) 479, 579, 1370
GlaI GCGC 1 cut(s) 1227
GluI GCNGC 4 cut(s) 9, 678, 942, 1205
HaeIII GGCC 2 cut(s) 161, 183
HapII CCGG 1 cut(s) 219
HgaI GACGC 2 cut(s) 151, 298
HhaI GCGC 1 cut(s) 1228
Hin1I GRCGYC 3 cut(s) 143, 171, 309
Hin1II CATG 7 cut(s) 431, 556, 634, 954, 1119, 1345, 1429
Hin6I GCGC 1 cut(s) 1226
HinP1I GCGC 1 cut(s) 1226
HincII GTYRAC 2 cut(s) 16, 544
HindII GTYRAC 2 cut(s) 16, 544
HindIII AAGCTT 1 cut(s) 682
HinfI GANTC 6 cut(s) 74, 385, 560, 881, 911, 921
HpaI GTTAAC 1 cut(s) 544
HpaII CCGG 1 cut(s) 219
HphI GGTGA 4 cut(s) 108, 178, 794, 1287
Hpy166II GTNNAC 5 cut(s) 16, 485, 544, 823, 1091
Hpy188I TCNGA 4 cut(s) 529, 880, 916, 936
Hpy188III TCNNGA 5 cut(s) 167, 331, 702, 746, 857
Hpy8I GTNNAC 5 cut(s) 16, 485, 544, 823, 1091
Hpy99I CGWCG 7 cut(s) 17, 20, 23, 145, 173, 194, 238
HpyCH4III ACNGT 3 cut(s) 190, 1198, 1449
HpyCH4IV ACGT 3 cut(s) 21, 171, 472
HpyF10VI GCNNNNNNNGC 4 cut(s) 224, 973, 1247, 1342
HpyF3I CTNAG 4 cut(s) 374, 526, 913, 1264
HpySE526I ACGT 3 cut(s) 21, 171, 472
Hsp92I GRCGYC 3 cut(s) 143, 171, 309
Hsp92II CATG 7 cut(s) 431, 556, 634, 954, 1119, 1345, 1429
HspAI GCGC 1 cut(s) 1226
KpnI GGTACC 1 cut(s) 1368
KspAI GTTAAC 1 cut(s) 544
Kzo9I GATC 4 cut(s) 93, 531, 778, 1349
LmnI GCTCC 3 cut(s) 31, 224, 1130
Lsp1109I GCAGC 4 cut(s) 20, 664, 928, 1191
LweI GCATC 5 cut(s) 124, 146, 205, 808, 1142
MabI ACCWGGT 1 cut(s) 706
MaeI CTAG 3 cut(s) 479, 579, 1370
MaeII ACGT 3 cut(s) 21, 171, 472
MaeIII GTNAC 7 cut(s) 262, 405, 434, 946, 1192, 1253, 1293
MalI GATC 4 cut(s) 95, 533, 780, 1351
MboI GATC 4 cut(s) 93, 531, 778, 1349
MfeI CAATTG 1 cut(s) 501
MflI RGATCY 2 cut(s) 93, 531
MhlI GDGCHC 2 cut(s) 111, 978
MluCI AATT 4 cut(s) 501, 688, 717, 850
MlyI GAGTC 1 cut(s) 905
MmeI TCCRAC 2 cut(s) 147, 683
Mph1103I ATGCAT 2 cut(s) 139, 1154
MseI TTAA 4 cut(s) 443, 543, 849, 926
MslI CAYNNNNRTG 2 cut(s) 432, 1190
MspCI CTTAAG 1 cut(s) 925
MspI CCGG 1 cut(s) 219
MspR9I CCNGG 2 cut(s) 158, 708
MunI CAATTG 1 cut(s) 501
Mva1269I GAATGC 3 cut(s) 326, 845, 1228
MvaI CCWGG 2 cut(s) 158, 708
MvnI CGCG 1 cut(s) 194
MwoI GCNNNNNNNGC 4 cut(s) 224, 973, 1247, 1342
NcoI CCATGG 1 cut(s) 427
NdeI CATATG 1 cut(s) 448
NdeII GATC 4 cut(s) 93, 531, 778, 1349
NlaIII CATG 7 cut(s) 431, 556, 634, 954, 1119, 1345, 1429
NlaIV GGNNCC 1 cut(s) 1366
NmuCI GTSAC 4 cut(s) 434, 1192, 1253, 1293
NsiI ATGCAT 2 cut(s) 139, 1154
NspI RCATGY 4 cut(s) 634, 954, 1119, 1345
PaeI GCATGC 1 cut(s) 1345
PciI ACATGT 3 cut(s) 630, 950, 1115
PctI GAATGC 3 cut(s) 326, 845, 1228
PfeI GAWTC 5 cut(s) 74, 385, 560, 881, 921
PkrI GCNGC 4 cut(s) 10, 679, 943, 1206
Ple19I CGATCG 2 cut(s) 781, 1352
PleI GAGTC 1 cut(s) 905
PpsI GAGTC 1 cut(s) 905
PscI ACATGT 3 cut(s) 630, 950, 1115
Psp6I CCWGG 2 cut(s) 156, 706
PspEI GGTNACC 1 cut(s) 1293
PspGI CCWGG 2 cut(s) 156, 706
PspN4I GGNNCC 1 cut(s) 1366
PspPI GGNCC 3 cut(s) 704, 866, 997
PstI CTGCAG 1 cut(s) 10
PstNI CAGNNNCTG 1 cut(s) 601
PsuI RGATCY 2 cut(s) 93, 531
PvuI CGATCG 2 cut(s) 781, 1352
RsaI GTAC 3 cut(s) 486, 712, 1366
RsaNI GTAC 3 cut(s) 485, 711, 1365
RseI CAYNNNNRTG 2 cut(s) 432, 1190
SalI GTCGAC 1 cut(s) 14
SaqAI TTAA 4 cut(s) 443, 543, 849, 926
SatI GCNGC 4 cut(s) 9, 678, 942, 1205
Sau3AI GATC 4 cut(s) 93, 531, 778, 1349
Sau96I GGNCC 3 cut(s) 704, 866, 997
SchI GAGTC 1 cut(s) 905
ScrFI CCNGG 2 cut(s) 158, 708
SduI GDGCHC 2 cut(s) 111, 978
SexAI ACCWGGT 1 cut(s) 706
SfaNI GCATC 5 cut(s) 124, 146, 205, 808, 1142
SfcI CTRYAG 3 cut(s) 6, 43, 488
SgrDI CGTCGACG 1 cut(s) 14
SinI GGWCC 3 cut(s) 704, 866, 997
SmiMI CAYNNNNRTG 2 cut(s) 432, 1190
SmlI CTYRAG 2 cut(s) 925, 1385
SmoI CTYRAG 2 cut(s) 925, 1385
SphI GCATGC 1 cut(s) 1345
Sse9I AATT 4 cut(s) 501, 688, 717, 850
SsiI CCGC 2 cut(s) 111, 1291
SspI AATATT 1 cut(s) 1050
SspMI CTAG 3 cut(s) 479, 579, 1370
StyD4I CCNGG 2 cut(s) 156, 706
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 3 cut(s) 190, 1198, 1449
TaiI ACGT 3 cut(s) 24, 174, 475
TasI AATT 4 cut(s) 501, 688, 717, 850
TatI WGTACW 1 cut(s) 484
TfiI GAWTC 5 cut(s) 74, 385, 560, 881, 921
Tru1I TTAA 4 cut(s) 443, 543, 849, 926
Tru9I TTAA 4 cut(s) 443, 543, 849, 926
TscAI CASTG 4 cut(s) 570, 602, 850, 1203
TseFI GTSAC 4 cut(s) 434, 1192, 1253, 1293
TseI GCWGC 4 cut(s) 8, 677, 941, 1204
Tsp45I GTSAC 4 cut(s) 434, 1192, 1253, 1293
TspDTI ATGAA 6 cut(s) 26, 398, 687, 1174, 1235, 1442
TspGWI ACGGA 3 cut(s) 84, 167, 240
TspRI CASTG 4 cut(s) 570, 602, 850, 1203
Vha464I CTTAAG 1 cut(s) 925
VpaK11BI GGWCC 3 cut(s) 704, 866, 997
XapI RAATTY 1 cut(s) 688
XceI RCATGY 4 cut(s) 634, 954, 1119, 1345
XcmI CCANNNNNNNNNTGG 1 cut(s) 446
XmiI GTMKAC 1 cut(s) 15
XspI CTAG 3 cut(s) 479, 579, 1370
ZraI GACGTC 1 cut(s) 172
Zsp2I ATGCAT 2 cut(s) 139, 1154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.