MD03G1298300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0016355
Physical Location & Seq
Forward (+)
37388496 .. 37390320
1825 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1298300.v1.1.491

Sequence Viewer

Length: 1275 bp
ATGAAGGACCAGCTACTATTTTCCAGTGATGAATCAACAACAGATCATCCTGATGACTATTCTAATTCTTCAAGATCAATAGCTCCTAATGATAATGATAGTGATAATAACAACTATTATTGGTGGAGATGGGCTGCAGAGTTTGATCATGTAAAGAAGATGCAGATGAAGCTTGATCATGAGCGCCTCATATTCCCTGGCGGTGGCAGGGCATCTAGTCATTTTACAGCAGCAGCAAGGATGCGAGTGGTGAGAGAGTTGGAGAGACTGGCTTTGGTATCAACCCATCAGGAAGGGGTGAATGAGCTTCGGCACAAGTTGCTCATGTATCGCTCGGGTGACTTCTGGATGCCAACAGGAGGTCTTGCTAAGGAAAACATGGACATTCCGCCGGTGGCCACAATTCTCTTGGTTGGCTTCTCTGCTTCTGGTAAAAGCTCTCTTGTCAACCTCATGTACTCCGTTTTGGGTCGCTCTGGACTCGTACCCTTTGCTCAAACATCACCACCACCCGGAAGCTTTTCAAATAATCCGAAGAGTACTAGTATGATTCTGAAAGAGCACAATGTGCTGAGGTCTTTGCGAAGTGGCTTCTGTGTGTATGACTCGAGGGGATTTGACTACAACCGAATAGGCGAGGGCCTTCAAGAATTGTCAAGTTGGATGAGTGATGGGATTCACCATAACCAAGTGTGCTTGAGACCGGGCGATGACGTCGAAGAATCTGTGTTGACGAATCTTCCTGATATGGATGTGATTTCAAAGTTTGCGAGGAGGAAAGTCAATTGTGCAATGGTGGTGGCTAACATGGCAGAAATCTACAAGGCCTTTCAAGCTGGTGATTCTAAGCCAATGGACGCCACAAAACAGCTTTTCTGCTCTGTCAATAATGCTTTCACCAATATTTACAATGATGAGAGCACCATTTTGATCTTGACACATGGTGACATGTTAACAACTGAGGAGAGGATTGAAGGGCGGCTGAAGATATGTGAGTGCCTCGGTGTATCGGAGACGAGTGGAGTATACGACATCGTAACGTGCCTTAGCGAGTATGGATTTCTGGCAGAAGAATCAGACCCGGTTTCAGCCTACGCCCTGAGTGAAGCAGTATATATGGCGTTGCTCCTATCAGACAGGACCCACTCTCCCAAAAAAACCCTTGGGGACTGGGCAGTGCTTATATTGTCCTCCATCATGTGCTTTCTTGCTTCTATATTTGGTCTCCTTGCTCAGATTTGCCTCACACTCGGTCGACACAGCAAAGATGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MMR_HSR1

Protein Analysis

425

Amino Acids

47.21

Weight (kDa)

5.49

Isoelectric Point (pI)

52.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016355)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g39660 FvH4_5g39660
malus_domestica MD03G1298300.v1.1
prunus_persica Prupe.8G269000_v2.0.a1
pyrus_communis pycom03g23620
rosa_chinensis RchiOBHm_Chr7g0241971
rosa_laevigata RLG00000000525
rosa_multiflora Rmu_sc0003266.1_g000005
rosa_roxburghii Rroxscaffold_3G00218420
rosa_rugosa Rorug07G0349400
rosa_samantha Rh7AG503400 Rh7BG474600 Rh7CG520200 Rh7DG489000
rosa_wichuraiana Rw7G042570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 717
AccI GTMKAC 2 cut(s) 1026, 1255
AciI CCGC 3 cut(s) 201, 389, 979
AcoI YGGCCR 1 cut(s) 396
AcuI CTGAAG 1 cut(s) 1004
AcyI GRCGYC 2 cut(s) 714, 858
AdeI CACNNNGTG 2 cut(s) 568, 944
AfaI GTAC 3 cut(s) 458, 486, 541
AfiI CCNNNNNNNGG 3 cut(s) 203, 359, 512
AflIII ACRYGT 1 cut(s) 948
AgsI TTSAA 6 cut(s) 72, 525, 647, 762, 833, 974
AhlI ACTAGT 1 cut(s) 542
AjnI CCWGG 1 cut(s) 196
AluBI AGCT 8 cut(s) 13, 83, 172, 307, 438, 519, 836, 871
AluI AGCT 8 cut(s) 13, 83, 172, 307, 438, 519, 836, 871
Alw21I GWGCWC 2 cut(s) 564, 923
Alw26I GTCTC 4 cut(s) 259, 694, 1007, 1229
Ama87I CYCGRG 2 cut(s) 334, 607
AoxI GGCC 3 cut(s) 396, 640, 825
ApeKI GCWGC 3 cut(s) 134, 230, 233
Asp700I GAANNNNTTC 1 cut(s) 520
AspLEI GCGC 1 cut(s) 186
AspS9I GGNCC 3 cut(s) 7, 640, 1140
AsuC2I CCSGG 3 cut(s) 513, 705, 1082
AsuHPI GGTGA 8 cut(s) 262, 310, 350, 495, 671, 851, 889, 956
AvaI CYCGRG 2 cut(s) 334, 607
AvaII GGWCC 2 cut(s) 7, 1140
BalI TGGCCA 1 cut(s) 398
Bbv12I GWGCWC 2 cut(s) 564, 923
BbvCI CCTCAGC 1 cut(s) 572
BbvI GCAGC 3 cut(s) 121, 242, 245
BccI CCATC 4 cut(s) 123, 294, 665, 1202
BciT130I CCWGG 1 cut(s) 198
BclI TGATCA 2 cut(s) 145, 175
BcnI CCSGG 3 cut(s) 513, 705, 1082
BcoDI GTCTC 4 cut(s) 259, 694, 1007, 1229
BcuI ACTAGT 1 cut(s) 542
BfaI CTAG 2 cut(s) 216, 543
BfmI CTRYAG 1 cut(s) 135
BfoI RGCGCY 1 cut(s) 187
BisI GCNGC 4 cut(s) 135, 231, 234, 980
BlsI GCNGC 4 cut(s) 136, 232, 235, 981
BmcAI AGTACT 1 cut(s) 541
Bme1390I CCNGG 4 cut(s) 198, 513, 705, 1082
Bme18I GGWCC 2 cut(s) 7, 1140
BmeT110I CYCGRG 2 cut(s) 334, 607
BmgT120I GGNCC 3 cut(s) 7, 640, 1140
BmiI GGNNCC 1 cut(s) 1142
BmrFI CCNGG 4 cut(s) 198, 513, 705, 1082
BmrI ACTGGG 1 cut(s) 1180
BmsI GCATC 4 cut(s) 150, 221, 231, 339
BmuI ACTGGG 1 cut(s) 1180
Bpu10I CCTNAGC 3 cut(s) 369, 572, 1046
BpuEI CTTGAG 1 cut(s) 718
BpuMI CCSGG 3 cut(s) 513, 705, 1082
BsaHI GRCGYC 2 cut(s) 714, 858
BsaI GGTCTC 2 cut(s) 694, 1229
BsaJI CCNNGG 3 cut(s) 196, 1000, 1162
BsaXI ACNNNNNCTCC 2 cut(s) 1132, 1162
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 359, 512
Bse118I RCCGGY 1 cut(s) 391
Bse1I ACTGG 3 cut(s) 24, 273, 1175
Bse3DI GCAATG 1 cut(s) 798
BseBI CCWGG 1 cut(s) 198
BseDI CCNNGG 3 cut(s) 196, 1000, 1162
BseGI GGATG 5 cut(s) 46, 246, 354, 669, 757
BseLI CCNNNNNNNGG 3 cut(s) 203, 359, 512
BseMI GCAATG 1 cut(s) 798
BseMII CTCAG 4 cut(s) 563, 951, 1091, 1247
BseNI ACTGG 3 cut(s) 24, 273, 1175
BseRI GAGGAG 2 cut(s) 787, 977
BseXI GCAGC 3 cut(s) 121, 242, 245
Bsh1285I CGRYCG 1 cut(s) 1255
BshFI GGCC 3 cut(s) 398, 642, 827
BsiEI CGRYCG 1 cut(s) 1255
BsiHKAI GWGCWC 2 cut(s) 564, 923
BsiHKCI CYCGRG 2 cut(s) 334, 607
BsiSI CCGG 4 cut(s) 392, 513, 704, 1082
BslFI GGGAC 1 cut(s) 1181
BslI CCNNNNNNNGG 3 cut(s) 203, 359, 512
BsmAI GTCTC 4 cut(s) 259, 694, 1007, 1229
BsmBI CGTCTC 1 cut(s) 1007
BsmFI GGGAC 1 cut(s) 1181
BsnI GGCC 3 cut(s) 398, 642, 827
Bso31I GGTCTC 2 cut(s) 694, 1229
BsoBI CYCGRG 2 cut(s) 334, 607
Bsp1286I GDGCHC 2 cut(s) 564, 923
Bsp143I GATC 5 cut(s) 43, 74, 145, 175, 930
BspACI CCGC 3 cut(s) 201, 389, 979
BspANI GGCC 3 cut(s) 398, 642, 827
BspCNI CTCAG 4 cut(s) 564, 952, 1092, 1246
BspHI TCATGA 1 cut(s) 178
BspLI GGNNCC 1 cut(s) 1142
BspMAI CTGCAG 1 cut(s) 139
BspTNI GGTCTC 2 cut(s) 694, 1229
BsrDI GCAATG 1 cut(s) 798
BsrFI RCCGGY 1 cut(s) 391
BsrI ACTGG 3 cut(s) 24, 273, 1175
BssAI RCCGGY 1 cut(s) 391
BssECI CCNNGG 3 cut(s) 196, 1000, 1162
BssMI GATC 5 cut(s) 43, 74, 145, 175, 930
BssNAI GTATAC 1 cut(s) 1027
BssNI GRCGYC 2 cut(s) 714, 858
BssT1I CCWWGG 1 cut(s) 1162
Bst1107I GTATAC 1 cut(s) 1027
Bst2UI CCWGG 1 cut(s) 198
Bst6I CTCTTC 1 cut(s) 530
BstACI GRCGYC 2 cut(s) 714, 858
BstAPI GCANNNNNTGC 2 cut(s) 319, 568
BstDEI CTNAG 7 cut(s) 369, 572, 846, 960, 1046, 1100, 1233
BstF5I GGATG 5 cut(s) 46, 246, 354, 669, 757
BstH2I RGCGCY 1 cut(s) 187
BstHHI GCGC 1 cut(s) 186
BstKTI GATC 5 cut(s) 46, 77, 148, 178, 933
BstMAI GTCTC 4 cut(s) 259, 694, 1007, 1229
BstMBI GATC 5 cut(s) 43, 74, 145, 175, 930
BstMCI CGRYCG 1 cut(s) 1255
BstMWI GCNNNNNNNGC 5 cut(s) 169, 319, 568, 809, 833
BstNI CCWGG 1 cut(s) 198
BstNSI RCATGY 1 cut(s) 952
BstSCI CCNGG 4 cut(s) 196, 511, 703, 1080
BstSFI CTRYAG 1 cut(s) 135
BstV1I GCAGC 3 cut(s) 121, 242, 245
BstZ17I GTATAC 1 cut(s) 1027
BsuRI GGCC 3 cut(s) 398, 642, 827
BtgZI GCGATG 1 cut(s) 723
BtsCI GGATG 5 cut(s) 46, 246, 354, 669, 757
BtsI GCAGTG 1 cut(s) 1182
BtsIMutI CAGTG 2 cut(s) 31, 1182
CciI TCATGA 1 cut(s) 178
CfoI GCGC 1 cut(s) 186
Cfr10I RCCGGY 1 cut(s) 391
Cfr13I GGNCC 3 cut(s) 7, 640, 1140
CseI GACGC 1 cut(s) 866
Csp6I GTAC 3 cut(s) 457, 485, 540
CspCI CAANNNNNGTGG 2 cut(s) 780, 815
CviAII CATG 9 cut(s) 149, 179, 325, 379, 454, 808, 941, 949, 1198
CviQI GTAC 3 cut(s) 457, 485, 540
DdeI CTNAG 7 cut(s) 369, 572, 846, 960, 1046, 1100, 1233
DpnI GATC 5 cut(s) 45, 76, 147, 177, 932
DpnII GATC 5 cut(s) 43, 74, 145, 175, 930
DraIII CACNNNGTG 2 cut(s) 568, 944
EaeI YGGCCR 1 cut(s) 396
Eam1104I CTCTTC 1 cut(s) 530
EarI CTCTTC 1 cut(s) 530
EciI GGCGGA 1 cut(s) 378
Eco130I CCWWGG 1 cut(s) 1162
Eco147I AGGCCT 1 cut(s) 827
Eco31I GGTCTC 2 cut(s) 694, 1229
Eco47I GGWCC 2 cut(s) 7, 1140
Eco57I CTGAAG 1 cut(s) 1004
Eco88I CYCGRG 2 cut(s) 334, 607
EcoO109I RGGNCCY 2 cut(s) 640, 1140
EcoRII CCWGG 1 cut(s) 196
EcoT14I CCWWGG 1 cut(s) 1162
ErhI CCWWGG 1 cut(s) 1162
Esp3I CGTCTC 1 cut(s) 1007
FaeI CATG 9 cut(s) 152, 182, 328, 382, 457, 811, 944, 952, 1201
FaqI GGGAC 1 cut(s) 1181
FatI CATG 9 cut(s) 148, 178, 324, 378, 453, 807, 940, 948, 1197
FbaI TGATCA 2 cut(s) 145, 175
FblI GTMKAC 2 cut(s) 1026, 1255
Fnu4HI GCNGC 4 cut(s) 135, 231, 234, 980
FokI GGATG 5 cut(s) 33, 253, 361, 676, 764
Fsp4HI GCNGC 4 cut(s) 135, 231, 234, 980
FspBI CTAG 2 cut(s) 216, 543
GlaI GCGC 1 cut(s) 185
GluI GCNGC 4 cut(s) 135, 231, 234, 980
HaeII RGCGCY 1 cut(s) 187
HaeIII GGCC 3 cut(s) 398, 642, 827
HapII CCGG 4 cut(s) 392, 513, 704, 1082
HgaI GACGC 1 cut(s) 866
HhaI GCGC 1 cut(s) 186
Hin1I GRCGYC 2 cut(s) 714, 858
Hin1II CATG 9 cut(s) 152, 182, 328, 382, 457, 811, 944, 952, 1201
Hin6I GCGC 1 cut(s) 184
HinP1I GCGC 1 cut(s) 184
HincII GTYRAC 4 cut(s) 448, 732, 954, 1256
HindII GTYRAC 4 cut(s) 448, 732, 954, 1256
HindIII AAGCTT 2 cut(s) 170, 517
HinfI GANTC 9 cut(s) 32, 480, 550, 605, 676, 722, 736, 842, 1073
HpaI GTTAAC 1 cut(s) 954
HpaII CCGG 4 cut(s) 392, 513, 704, 1082
HphI GGTGA 8 cut(s) 262, 310, 350, 495, 671, 851, 889, 956
Hpy166II GTNNAC 5 cut(s) 448, 732, 954, 1027, 1256
Hpy188I TCNGA 6 cut(s) 534, 555, 1012, 1078, 1135, 1236
Hpy188III TCNNGA 9 cut(s) 50, 72, 179, 290, 346, 477, 647, 743, 934
Hpy8I GTNNAC 5 cut(s) 448, 732, 954, 1027, 1256
Hpy99I CGWCG 1 cut(s) 719
HpyAV CCTTC 3 cut(s) 287, 653, 968
HpyCH4IV ACGT 2 cut(s) 714, 1040
HpyCH4V TGCA 3 cut(s) 137, 163, 791
HpyF10VI GCNNNNNNNGC 5 cut(s) 169, 319, 568, 809, 833
HpyF3I CTNAG 7 cut(s) 369, 572, 846, 960, 1046, 1100, 1233
HpySE526I ACGT 2 cut(s) 714, 1040
Hsp92I GRCGYC 2 cut(s) 714, 858
Hsp92II CATG 9 cut(s) 152, 182, 328, 382, 457, 811, 944, 952, 1201
HspAI GCGC 1 cut(s) 184
Ksp22I TGATCA 2 cut(s) 145, 175
KspAI GTTAAC 1 cut(s) 954
Kzo9I GATC 5 cut(s) 43, 74, 145, 175, 930
LmnI GCTCC 2 cut(s) 88, 1131
Lsp1109I GCAGC 3 cut(s) 121, 242, 245
LweI GCATC 4 cut(s) 150, 221, 231, 339
MaeI CTAG 2 cut(s) 216, 543
MaeII ACGT 2 cut(s) 714, 1040
MaeIII GTNAC 3 cut(s) 338, 944, 1036
MalI GATC 5 cut(s) 45, 76, 147, 177, 932
MboI GATC 5 cut(s) 43, 74, 145, 175, 930
MboII GAAGA 7 cut(s) 60, 169, 547, 731, 731, 997, 1082
MfeI CAATTG 1 cut(s) 784
MhlI GDGCHC 2 cut(s) 564, 923
MlsI TGGCCA 1 cut(s) 398
MluCI AATT 4 cut(s) 64, 402, 650, 784
MluNI TGGCCA 1 cut(s) 398
MlyI GAGTC 2 cut(s) 474, 599
MmeI TCCRAC 2 cut(s) 240, 641
Mox20I TGGCCA 1 cut(s) 398
MroXI GAANNNNTTC 1 cut(s) 520
MscI TGGCCA 1 cut(s) 398
MseI TTAA 1 cut(s) 953
MslI CAYNNNNRTG 1 cut(s) 51
Msp20I TGGCCA 1 cut(s) 398
MspI CCGG 4 cut(s) 392, 513, 704, 1082
MspR9I CCNGG 4 cut(s) 198, 513, 705, 1082
MunI CAATTG 1 cut(s) 784
MvaI CCWGG 1 cut(s) 198
MwoI GCNNNNNNNGC 5 cut(s) 169, 319, 568, 809, 833
NciI CCSGG 3 cut(s) 513, 705, 1082
NdeII GATC 5 cut(s) 43, 74, 145, 175, 930
NlaIII CATG 9 cut(s) 152, 182, 328, 382, 457, 811, 944, 952, 1201
NlaIV GGNNCC 1 cut(s) 1142
NmuCI GTSAC 2 cut(s) 338, 944
NspI RCATGY 1 cut(s) 952
PaeR7I CTCGAG 1 cut(s) 607
PagI TCATGA 1 cut(s) 178
PceI AGGCCT 1 cut(s) 827
PciI ACATGT 1 cut(s) 948
PdmI GAANNNNTTC 1 cut(s) 520
PfeI GAWTC 7 cut(s) 32, 550, 676, 722, 736, 842, 1073
PkrI GCNGC 4 cut(s) 136, 232, 235, 981
PleI GAGTC 2 cut(s) 474, 599
PpsI GAGTC 2 cut(s) 474, 599
PpuMI RGGWCCY 1 cut(s) 1140
PscI ACATGT 1 cut(s) 948
Psp5II RGGWCCY 1 cut(s) 1140
Psp6I CCWGG 1 cut(s) 196
PspGI CCWGG 1 cut(s) 196
PspN4I GGNNCC 1 cut(s) 1142
PspPI GGNCC 3 cut(s) 7, 640, 1140
PspPPI RGGWCCY 1 cut(s) 1140
PspXI VCTCGAGB 1 cut(s) 607
PstI CTGCAG 1 cut(s) 139
RsaI GTAC 3 cut(s) 458, 486, 541
RsaNI GTAC 3 cut(s) 457, 485, 540
RseI CAYNNNNRTG 1 cut(s) 51
SalI GTCGAC 1 cut(s) 1254
SaqAI TTAA 1 cut(s) 953
SatI GCNGC 4 cut(s) 135, 231, 234, 980
Sau3AI GATC 5 cut(s) 43, 74, 145, 175, 930
Sau96I GGNCC 3 cut(s) 7, 640, 1140
ScaI AGTACT 1 cut(s) 541
SchI GAGTC 2 cut(s) 474, 599
ScrFI CCNGG 4 cut(s) 198, 513, 705, 1082
SduI GDGCHC 2 cut(s) 564, 923
SfaNI GCATC 4 cut(s) 150, 221, 231, 339
SfcI CTRYAG 1 cut(s) 135
Sfr274I CTCGAG 1 cut(s) 607
SgrAI CRCCGGYG 1 cut(s) 391
SinI GGWCC 2 cut(s) 7, 1140
SlaI CTCGAG 1 cut(s) 607
SmiMI CAYNNNNRTG 1 cut(s) 51
SmlI CTYRAG 2 cut(s) 607, 697
SmoI CTYRAG 2 cut(s) 607, 697
SpeI ACTAGT 1 cut(s) 542
Sse9I AATT 4 cut(s) 64, 402, 650, 784
SseBI AGGCCT 1 cut(s) 827
SsiI CCGC 3 cut(s) 201, 389, 979
SspI AATATT 1 cut(s) 904
SspMI CTAG 2 cut(s) 216, 543
StuI AGGCCT 1 cut(s) 827
StyD4I CCNGG 4 cut(s) 196, 511, 703, 1080
StyI CCWWGG 1 cut(s) 1162
TaiI ACGT 2 cut(s) 717, 1043
TaqI TCGA 3 cut(s) 608, 717, 1255
TaqII GACCGA 1 cut(s) 1241
TasI AATT 4 cut(s) 64, 402, 650, 784
TatI WGTACW 2 cut(s) 456, 539
TauI GCSGC 1 cut(s) 982
TfiI GAWTC 7 cut(s) 32, 550, 676, 722, 736, 842, 1073
Tru1I TTAA 1 cut(s) 953
Tru9I TTAA 1 cut(s) 953
TscAI CASTG 2 cut(s) 31, 1182
TseFI GTSAC 2 cut(s) 338, 944
TseI GCWGC 3 cut(s) 134, 230, 233
Tsp45I GTSAC 2 cut(s) 338, 944
TspDTI ATGAA 3 cut(s) 17, 45, 182
TspGWI ACGGA 1 cut(s) 451
TspRI CASTG 2 cut(s) 31, 1182
VpaK11BI GGWCC 2 cut(s) 7, 1140
XceI RCATGY 1 cut(s) 952
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
XhoI CTCGAG 1 cut(s) 607
XmiI GTMKAC 2 cut(s) 1026, 1255
XmnI GAANNNNTTC 1 cut(s) 520
XspI CTAG 2 cut(s) 216, 543
ZraI GACGTC 1 cut(s) 715
ZrmI AGTACT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.