Rroxscaffold_3G00218420

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0016355
Physical Location & Seq
Reverse (-)
614432 .. 616372
1941 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00218420.1

Sequence Viewer

Length: 1293 bp
ATGAAGAACCATATGCATGTATGTAATTCGAGCAGTGACGATGAGAAGATTGTAATTAGTGATGAGTCGTCAGGATCAGTATCATCAGTAGAACCTTACTGCTGGTGGAGATCGGCGGCAAAGTACGATGAGTATGTTAAGCTCCAGTTTGAGGGCGGCCTGCCAGACGTGTCAAGGCTGACAACAAGGATGAGAGTGTTCAAAGAGTTGGAGAGATTAGCTTTGGTTGCTAGTGGCTCTACTAGTACTCGTGAATATCATTATCATTACAGTGAAGGTGGTGTGAATGAGCTCAGGCACAAGCTCCTCACGTATCGATCAGGAGATTTCTGGATGCCTATTGGAGGAATTAGTAAGGAGAAGATGGACATTCCACCAGTGGCCACAATTCTCTTGGTCGGCTTCTCTGCTTCCGGCAAGAGCTCGCTTGTCAATCTAATGTATAGTGTTTTGGGTCGCTCTGGACTCATACCTTTTGCTCAAACATCCTCCTCCTCAGGAAGTGGTAGAACCAAGCTTTTGAAAGAGCACAATGTGGTGAGGTCTTTGCGTAGTGGGTTTTGCGTGTACGACTCAGGGGGATTCGATTACAATGACATCAGTGAAAGTAGTGTTGGTGAAGAATTGTCAAGTTGGATGAATGATGGGGTTCACCAGAACCAAGAATGCTTAAGATCGGGAGATCTGGACGACGTCCCTCATCATGATGTTGCAGGTGATGATAGGCATGTCGATTTTCGAACTATGTCAAATTATAATTGGTCTTCAAAGTTTGTGAGGAGGACAGTGAATTCTGTTATCGTGGTGGCTAACATGGCAGAGATCTACAAGGCATTTAAAGCTGGTGATTCTAAGCCAATGGAGGCCACAAGACAGCTTTTCTGCTCAAGTGCATTCCGAAATTGCAATGATGAGAAGCCTATCTTGATCTTGACACATGGTGACATGTTATCAACTGAAGAAAGGATTGAAGGGCGGCTTAAAATATGTGAATGTGTAGGTGTATCAGAGACGACCGGAGTATATGACATCGTGTGTCTTACCGAGTATGGATTTCTCGCAGAAGAATCAGACCCGGTTTCAGCTTATGCCCTCACAGAAGCAGTATATAGGGCATTGCTCATATCGGACAGGACCCACTTACCGAAGAAAACACATCAGGACTGGGCATTGCTTGTATTATCCTGGAGTTTGTGCTTCCTTGCTTCCCTCTTTGCTCTCATTGCTGAGATTTGCTTCAAACTCGGAGGAGGTGGACGTCAGAGGGATCGACCCAAGCTACTCATGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MMR_HSR1

Protein Analysis

430

Amino Acids

48.03

Weight (kDa)

6.03

Isoelectric Point (pI)

50.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016355)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g39660 FvH4_5g39660
malus_domestica MD03G1298300.v1.1
prunus_persica Prupe.8G269000_v2.0.a1
pyrus_communis pycom03g23620
rosa_chinensis RchiOBHm_Chr7g0241971
rosa_laevigata RLG00000000525
rosa_multiflora Rmu_sc0003266.1_g000005
rosa_roxburghii Rroxscaffold_3G00218420
rosa_rugosa Rorug07G0349400
rosa_samantha Rh7AG503400 Rh7BG474600 Rh7CG520200 Rh7DG489000
rosa_wichuraiana Rw7G042570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 756
AarI CACCTGC 1 cut(s) 704
AatII GACGTC 2 cut(s) 696, 1261
Acc36I ACCTGC 1 cut(s) 704
AciI CCGC 3 cut(s) 116, 156, 976
AclWI GGATC 2 cut(s) 82, 1275
AcoI YGGCCR 1 cut(s) 381
AcsI RAATTY 1 cut(s) 790
AcuI CTGAAG 1 cut(s) 978
AcyI GRCGYC 2 cut(s) 693, 1258
AdeI CACNNNGTG 2 cut(s) 535, 941
AfaI GTAC 3 cut(s) 125, 247, 569
AfiI CCNNNNNNNGG 2 cut(s) 151, 344
AflII CTTAAG 1 cut(s) 670
AflIII ACRYGT 2 cut(s) 168, 945
AgsI TTSAA 5 cut(s) 202, 523, 768, 971, 1240
AhlI ACTAGT 1 cut(s) 242
AjiI CACGTC 1 cut(s) 169
AjnI CCWGG 1 cut(s) 1184
AjuI GAANNNNNNNTTGG 2 cut(s) 597, 629
Alw21I GWGCWC 3 cut(s) 294, 425, 531
Alw26I GTCTC 1 cut(s) 1004
AlwI GGATC 2 cut(s) 82, 1275
AoxI GGCC 3 cut(s) 157, 381, 864
ApoI RAATTY 1 cut(s) 790
AspS9I GGNCC 1 cut(s) 1134
AsuC2I CCSGG 1 cut(s) 1076
AsuHPI GGTGA 6 cut(s) 550, 629, 644, 728, 857, 953
AsuII TTCGAA 1 cut(s) 739
AvaII GGWCC 1 cut(s) 1134
AxyI CCTNAGG 1 cut(s) 496
BalI TGGCCA 1 cut(s) 383
BanII GRGCYC 2 cut(s) 294, 425
BauI CACGAG 1 cut(s) 249
BbsI GAAGAC 1 cut(s) 756
Bbv12I GWGCWC 3 cut(s) 294, 425, 531
BccI CCATC 2 cut(s) 358, 638
BciT130I CCWGG 1 cut(s) 1186
BcnI CCSGG 1 cut(s) 1076
BcoDI GTCTC 1 cut(s) 1004
BcuI ACTAGT 1 cut(s) 242
BfaI CTAG 2 cut(s) 231, 243
BfrI CTTAAG 1 cut(s) 670
BfuAI ACCTGC 1 cut(s) 704
BglII AGATCT 2 cut(s) 682, 822
BisI GCNGC 3 cut(s) 117, 157, 977
BlsI GCNGC 3 cut(s) 118, 158, 978
BmcAI AGTACT 1 cut(s) 247
Bme1390I CCNGG 2 cut(s) 1076, 1186
Bme18I GGWCC 1 cut(s) 1134
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 1134
BmiI GGNNCC 1 cut(s) 1136
BmrFI CCNGG 2 cut(s) 1076, 1186
BmrI ACTGGG 1 cut(s) 1174
BmsI GCATC 1 cut(s) 324
BmuI ACTGGG 1 cut(s) 1174
BpiI GAAGAC 1 cut(s) 756
BpmI CTGGAG 2 cut(s) 128, 1207
Bpu10I CCTNAGC 1 cut(s) 293
Bpu14I TTCGAA 1 cut(s) 739
BpuEI CTTGAG 1 cut(s) 871
BpuMI CCSGG 1 cut(s) 1076
Bsa29I ATCGAT 1 cut(s) 316
BsaAI YACGTR 1 cut(s) 312
BsaBI GATNNNNATC 1 cut(s) 79
BsaHI GRCGYC 2 cut(s) 693, 1258
BsaWI WCCGGW 1 cut(s) 1016
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 344
Bse1I ACTGG 3 cut(s) 145, 377, 1169
Bse21I CCTNAGG 1 cut(s) 496
Bse3DI GCAATG 4 cut(s) 913, 1115, 1169, 1221
Bse8I GATNNNNATC 1 cut(s) 79
BseBI CCWGG 1 cut(s) 1186
BseCI ATCGAT 1 cut(s) 316
BseGI GGATG 4 cut(s) 195, 339, 485, 642
BseJI GATNNNNATC 1 cut(s) 79
BseLI CCNNNNNNNGG 2 cut(s) 151, 344
BseMI GCAATG 4 cut(s) 913, 1115, 1169, 1221
BseMII CTCAG 4 cut(s) 307, 510, 588, 1218
BseNI ACTGG 3 cut(s) 145, 377, 1169
BseRI GAGGAG 5 cut(s) 296, 481, 484, 793, 1263
Bsh1285I CGRYCG 1 cut(s) 1017
BshFI GGCC 3 cut(s) 159, 383, 866
BshVI ATCGAT 1 cut(s) 316
BsiEI CGRYCG 1 cut(s) 1017
BsiHKAI GWGCWC 3 cut(s) 294, 425, 531
BsiSI CCGG 3 cut(s) 414, 1017, 1076
BslFI GGGAC 1 cut(s) 680
BslI CCNNNNNNNGG 2 cut(s) 151, 344
BsmAI GTCTC 1 cut(s) 1004
BsmBI CGTCTC 1 cut(s) 1004
BsmFI GGGAC 1 cut(s) 680
BsmI GAATGC 2 cut(s) 671, 893
BsnI GGCC 3 cut(s) 159, 383, 866
Bsp119I TTCGAA 1 cut(s) 739
Bsp1286I GDGCHC 3 cut(s) 294, 425, 531
Bsp143I GATC 8 cut(s) 74, 110, 317, 674, 682, 822, 927, 1267
BspACI CCGC 3 cut(s) 116, 156, 976
BspANI GGCC 3 cut(s) 159, 383, 866
BspCNI CTCAG 4 cut(s) 306, 509, 587, 1219
BspDI ATCGAT 1 cut(s) 316
BspHI TCATGA 2 cut(s) 703, 1284
BspLI GGNNCC 1 cut(s) 1136
BspMI ACCTGC 1 cut(s) 704
BspPI GGATC 2 cut(s) 82, 1275
BspT104I TTCGAA 1 cut(s) 739
BspTI CTTAAG 1 cut(s) 670
BsrDI GCAATG 4 cut(s) 913, 1115, 1169, 1221
BsrI ACTGG 3 cut(s) 145, 377, 1169
BssMI GATC 8 cut(s) 74, 110, 317, 674, 682, 822, 927, 1267
BssNI GRCGYC 2 cut(s) 693, 1258
BssSI CACGAG 1 cut(s) 249
Bst2BI CACGAG 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 1186
Bst4CI ACNGT 2 cut(s) 272, 787
BstACI GRCGYC 2 cut(s) 693, 1258
BstAFI CTTAAG 1 cut(s) 670
BstBAI YACGTR 1 cut(s) 312
BstBI TTCGAA 1 cut(s) 739
BstC8I GCNNGC 2 cut(s) 161, 425
BstDEI CTNAG 5 cut(s) 293, 496, 574, 852, 1227
BstENI CCTNNNNNAGG 1 cut(s) 342
BstF5I GGATG 4 cut(s) 195, 339, 485, 642
BstKTI GATC 8 cut(s) 77, 113, 320, 677, 685, 825, 930, 1270
BstMAI GTCTC 1 cut(s) 1004
BstMBI GATC 8 cut(s) 74, 110, 317, 674, 682, 822, 927, 1267
BstMCI CGRYCG 1 cut(s) 1017
BstMWI GCNNNNNNNGC 4 cut(s) 227, 815, 839, 1223
BstNI CCWGG 1 cut(s) 1186
BstNSI RCATGY 3 cut(s) 20, 731, 949
BstSCI CCNGG 2 cut(s) 1074, 1184
BstV2I GAAGAC 1 cut(s) 756
BstX2I RGATCY 2 cut(s) 682, 822
BstYI RGATCY 2 cut(s) 682, 822
Bsu15I ATCGAT 1 cut(s) 316
Bsu36I CCTNAGG 1 cut(s) 496
BsuRI GGCC 3 cut(s) 159, 383, 866
BsuTUI ATCGAT 1 cut(s) 316
BtrI CACGTC 1 cut(s) 169
BtsCI GGATG 4 cut(s) 195, 339, 485, 642
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 5 cut(s) 40, 277, 384, 607, 792
BveI ACCTGC 1 cut(s) 704
Cac8I GCNNGC 2 cut(s) 161, 425
CciI TCATGA 2 cut(s) 703, 1284
Cfr13I GGNCC 1 cut(s) 1134
ClaI ATCGAT 1 cut(s) 316
Csp6I GTAC 3 cut(s) 124, 246, 568
CviAII CATG 7 cut(s) 17, 704, 728, 814, 938, 946, 1285
CviQI GTAC 3 cut(s) 124, 246, 568
DdeI CTNAG 5 cut(s) 293, 496, 574, 852, 1227
DpnI GATC 8 cut(s) 76, 112, 319, 676, 684, 824, 929, 1269
DpnII GATC 8 cut(s) 74, 110, 317, 674, 682, 822, 927, 1267
DraI TTTAAA 1 cut(s) 838
DraIII CACNNNGTG 2 cut(s) 535, 941
EaeI YGGCCR 1 cut(s) 381
Ecl136II GAGCTC 2 cut(s) 292, 423
Eco24I GRGCYC 2 cut(s) 294, 425
Eco47I GGWCC 1 cut(s) 1134
Eco53kI GAGCTC 2 cut(s) 292, 423
Eco57I CTGAAG 1 cut(s) 978
Eco81I CCTNAGG 1 cut(s) 496
EcoICRI GAGCTC 2 cut(s) 292, 423
EcoNI CCTNNNNNAGG 1 cut(s) 342
EcoO109I RGGNCCY 1 cut(s) 1134
EcoRI GAATTC 1 cut(s) 790
EcoRII CCWGG 1 cut(s) 1184
EcoT22I ATGCAT 1 cut(s) 18
EcoT38I GRGCYC 2 cut(s) 294, 425
Esp3I CGTCTC 1 cut(s) 1004
FaeI CATG 7 cut(s) 20, 707, 731, 817, 941, 949, 1288
FalI AAGNNNNNCTT 4 cut(s) 908, 940, 963, 995
FaqI GGGAC 1 cut(s) 680
FatI CATG 7 cut(s) 16, 703, 727, 813, 937, 945, 1284
FauNDI CATATG 1 cut(s) 12
Fnu4HI GCNGC 3 cut(s) 117, 157, 977
FokI GGATG 4 cut(s) 202, 346, 472, 649
FriOI GRGCYC 2 cut(s) 294, 425
Fsp4HI GCNGC 3 cut(s) 117, 157, 977
FspBI CTAG 2 cut(s) 231, 243
GluI GCNGC 3 cut(s) 117, 157, 977
GsuI CTGGAG 2 cut(s) 128, 1207
HaeIII GGCC 3 cut(s) 159, 383, 866
HapII CCGG 3 cut(s) 414, 1017, 1076
Hin1I GRCGYC 2 cut(s) 693, 1258
Hin1II CATG 7 cut(s) 20, 707, 731, 817, 941, 949, 1288
HindIII AAGCTT 1 cut(s) 515
HinfI GANTC 6 cut(s) 65, 465, 572, 582, 848, 1067
HpaII CCGG 3 cut(s) 414, 1017, 1076
HphI GGTGA 6 cut(s) 550, 629, 644, 728, 857, 953
Hpy166II GTNNAC 3 cut(s) 568, 652, 1256
Hpy188I TCNGA 6 cut(s) 899, 1009, 1072, 1129, 1247, 1263
Hpy8I GTNNAC 3 cut(s) 568, 652, 1256
Hpy99I CGWCG 1 cut(s) 695
HpyAV CCTTC 2 cut(s) 269, 965
HpyCH4III ACNGT 2 cut(s) 272, 787
HpyCH4IV ACGT 4 cut(s) 168, 311, 693, 1258
HpyCH4V TGCA 4 cut(s) 16, 713, 893, 906
HpyF10VI GCNNNNNNNGC 4 cut(s) 227, 815, 839, 1223
HpyF3I CTNAG 5 cut(s) 293, 496, 574, 852, 1227
HpySE526I ACGT 4 cut(s) 168, 311, 693, 1258
Hsp92I GRCGYC 2 cut(s) 693, 1258
Hsp92II CATG 7 cut(s) 20, 707, 731, 817, 941, 949, 1288
Kzo9I GATC 8 cut(s) 74, 110, 317, 674, 682, 822, 927, 1267
LmnI GCTCC 2 cut(s) 147, 309
LweI GCATC 1 cut(s) 324
MaeI CTAG 2 cut(s) 231, 243
MaeII ACGT 4 cut(s) 168, 311, 693, 1258
MaeIII GTNAC 2 cut(s) 35, 941
MalI GATC 8 cut(s) 76, 112, 319, 676, 684, 824, 929, 1269
MboI GATC 8 cut(s) 74, 110, 317, 674, 682, 822, 927, 1267
MboII GAAGA 8 cut(s) 16, 58, 373, 632, 756, 971, 1076, 1159
MflI RGATCY 2 cut(s) 682, 822
MhlI GDGCHC 3 cut(s) 294, 425, 531
MlsI TGGCCA 1 cut(s) 383
MluCI AATT 9 cut(s) 25, 54, 348, 387, 623, 751, 757, 790, 901
MluNI TGGCCA 1 cut(s) 383
MlyI GAGTC 3 cut(s) 74, 459, 566
MmeI TCCRAC 2 cut(s) 189, 614
Mox20I TGGCCA 1 cut(s) 383
Mph1103I ATGCAT 1 cut(s) 18
MscI TGGCCA 1 cut(s) 383
MseI TTAA 4 cut(s) 138, 671, 837, 981
MslI CAYNNNNRTG 3 cut(s) 15, 270, 705
Msp20I TGGCCA 1 cut(s) 383
MspCI CTTAAG 1 cut(s) 670
MspI CCGG 3 cut(s) 414, 1017, 1076
MspR9I CCNGG 2 cut(s) 1076, 1186
Mva1269I GAATGC 2 cut(s) 671, 893
MvaI CCWGG 1 cut(s) 1186
MwoI GCNNNNNNNGC 4 cut(s) 227, 815, 839, 1223
NciI CCSGG 1 cut(s) 1076
NdeI CATATG 1 cut(s) 12
NdeII GATC 8 cut(s) 74, 110, 317, 674, 682, 822, 927, 1267
NlaIII CATG 7 cut(s) 20, 707, 731, 817, 941, 949, 1288
NlaIV GGNNCC 1 cut(s) 1136
NmuCI GTSAC 2 cut(s) 35, 941
NsiI ATGCAT 1 cut(s) 18
NspI RCATGY 3 cut(s) 20, 731, 949
NspV TTCGAA 1 cut(s) 739
PagI TCATGA 2 cut(s) 703, 1284
PaqCI CACCTGC 1 cut(s) 704
PciI ACATGT 1 cut(s) 945
PctI GAATGC 2 cut(s) 671, 893
PfeI GAWTC 3 cut(s) 582, 848, 1067
PflFI GACNNNGTC 1 cut(s) 692
PfoI TCCNGGA 1 cut(s) 1184
PkrI GCNGC 3 cut(s) 118, 158, 978
PleI GAGTC 3 cut(s) 73, 459, 566
PpsI GAGTC 3 cut(s) 73, 459, 566
Ppu21I YACGTR 1 cut(s) 312
PpuMI RGGWCCY 1 cut(s) 1134
PscI ACATGT 1 cut(s) 945
PsiI TTATAA 1 cut(s) 756
Psp124BI GAGCTC 2 cut(s) 294, 425
Psp5II RGGWCCY 1 cut(s) 1134
Psp6I CCWGG 1 cut(s) 1184
PspGI CCWGG 1 cut(s) 1184
PspN4I GGNNCC 1 cut(s) 1136
PspPI GGNCC 1 cut(s) 1134
PspPPI RGGWCCY 1 cut(s) 1134
PsuI RGATCY 2 cut(s) 682, 822
PsyI GACNNNGTC 1 cut(s) 692
RsaI GTAC 3 cut(s) 125, 247, 569
RsaNI GTAC 3 cut(s) 124, 246, 568
RseI CAYNNNNRTG 3 cut(s) 15, 270, 705
SacI GAGCTC 2 cut(s) 294, 425
SaqAI TTAA 4 cut(s) 138, 671, 837, 981
SatI GCNGC 3 cut(s) 117, 157, 977
Sau3AI GATC 8 cut(s) 74, 110, 317, 674, 682, 822, 927, 1267
Sau96I GGNCC 1 cut(s) 1134
ScaI AGTACT 1 cut(s) 247
SchI GAGTC 3 cut(s) 74, 459, 566
ScrFI CCNGG 2 cut(s) 1076, 1186
SduI GDGCHC 3 cut(s) 294, 425, 531
SfaNI GCATC 1 cut(s) 324
SfuI TTCGAA 1 cut(s) 739
SinI GGWCC 1 cut(s) 1134
SmiMI CAYNNNNRTG 3 cut(s) 15, 270, 705
SmlI CTYRAG 2 cut(s) 670, 886
SmoI CTYRAG 2 cut(s) 670, 886
SpeI ACTAGT 1 cut(s) 242
Sse9I AATT 9 cut(s) 25, 54, 348, 387, 623, 751, 757, 790, 901
SsiI CCGC 3 cut(s) 116, 156, 976
SspMI CTAG 2 cut(s) 231, 243
SstI GAGCTC 2 cut(s) 294, 425
StyD4I CCNGG 2 cut(s) 1074, 1184
TaaI ACNGT 2 cut(s) 272, 787
TaiI ACGT 4 cut(s) 171, 314, 696, 1261
TaqI TCGA 6 cut(s) 29, 316, 585, 732, 739, 1270
TasI AATT 9 cut(s) 25, 54, 348, 387, 623, 751, 757, 790, 901
TatI WGTACW 1 cut(s) 245
TauI GCSGC 3 cut(s) 119, 159, 979
TfiI GAWTC 3 cut(s) 582, 848, 1067
Tru1I TTAA 4 cut(s) 138, 671, 837, 981
Tru9I TTAA 4 cut(s) 138, 671, 837, 981
TscAI CASTG 5 cut(s) 40, 277, 384, 607, 792
TseFI GTSAC 2 cut(s) 35, 941
Tsp45I GTSAC 2 cut(s) 35, 941
TspDTI ATGAA 2 cut(s) 17, 653
TspRI CASTG 5 cut(s) 40, 277, 384, 607, 792
Tth111I GACNNNGTC 1 cut(s) 692
Vha464I CTTAAG 1 cut(s) 670
VpaK11BI GGWCC 1 cut(s) 1134
XagI CCTNNNNNAGG 1 cut(s) 342
XapI RAATTY 1 cut(s) 790
XceI RCATGY 3 cut(s) 20, 731, 949
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XspI CTAG 2 cut(s) 231, 243
ZraI GACGTC 2 cut(s) 694, 1259
ZrmI AGTACT 1 cut(s) 247
Zsp2I ATGCAT 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.