Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0012546

Physical Location & Seq

Genomic Coordinates Forward (+)

142870 .. 146508

Gene Structure 3639 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1001100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1503 bp

ATGATGGGGATGATGAGGATGATAAGGGTGGCCTGTTTGGTAACAATGATAGTGGTGCTCATGTGGAGCAGCAGCACCAACACCAACAGCAGACTGAGAATGGTGAGAATGGTGAGCGGGATTGGAGCCAACTGGGGGACACAGGCATCCCACCCTCTCCCCCCAGACACCGTAGTCCGGCTTCTCAAAGACAACGGCTTTCAGCGGGTTAAGCTTTTCGATGCTGACTACGACACTCTCAACAGTCTCGCTAAGTCTGGCATTGAGGTCATGGTCGGCATTCCCAACGATATGCTCGCCTCTCTCGCCACCAGCGTCAAGGCTGCTGAGAAATGGGTTTCCAAGAACGTCTCTGCCCACATCTCCTCCAACAATGTCAAGATCAGGTATGTTGCTGTTGGAAATGAGCCTTTCTTGGAAACATACAACGGTAGCTTCCTCAGAACAACCTTCCCTGCACTGCAGAATGTCCAGTCTGCCCTGATAAAAGCTGGCCTTAGCAACCAAGTAAAGGTGACTGTACCCCAGAACGCTGACGTGTATGCGAGTTCAAATGGATTTCCATCCGGTGGTGACTTCCGAACTGATATCCATGATTACATGATTCAAATTGTGAAGTTCTTGAGTGACAATGGTTGCCCCTTCACCGTGAACATTTACCCATTTATAAGCCTTTACATTGACTCCAACTTCCCTGTTGATTATGCCTTCTTTGATGGCAATGCCTCACCTATTACTGATGGTAGTGCAACATACTACAATATGTTTGACGCAAACTATGATACTCTTGTGTGGACCCTGCAGAAGAATGGCTTTGGAAATTTACCAATCATAGTTGGAGAGATTGGATGGCCGACAGATGGCGACCGGAATGCTAATCTAGTTTATGCTCAGAGGTTCAACCAAGGTTTCATGTCCCACATATTGGGTGGCAGAGGAACCCCAATGAGACCAGGGCCAATTGATGCTTATCTGTTCAGTTTGATTGACGAGGATGCTAAAAGCATTGATCCAGGAAACTTTGAACGACACTGGGGAATATTTACCTTTGATGGGTTAGCAAAATACCAGCTTAACCTTGGTTCCACAAATTCAGGGGCATTAGTTCCAGCAAAAGGTGTGCACTATTTGGAGCGTAAGTGGTGCGTGATGAAGCCTTCAGCAAGCCTTGATGACCCACAAGTAGCACTAAGTGTGAGCTATGCCTGTGGACTTGCAGATTGTACTAGCCTTGGTTACGGGACATCTTGTGGAAACTTGGATGATCGAGCAAACATTTCGTATGCTGTTAACAGTTACTACCAGAGGAACAATCAACTTGATGAAGCTTGCAAGTTTCCAAACCTTTCAATGATCACCAAAACAAACCCATCTGTCGGATCATGCAGATTTGAGATAATGATCGAACCATATTACGGAGCTGCAGAAAGGAGATCCGGGCATCTCCAAAAACCTCTGAGCCTGGTTGCACTTTTCGCTTTCTTGATGCTAACACTTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001871 GO:0003674 GO:0003824 GO:0004553 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0016787 GO:0016798 GO:0030054 GO:0030246 GO:0030247 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00500 map00500

Pfam Domains

Protein Families

Glyco_hydro_17 X8

Protein Analysis

501

Amino Acids

55.04

Weight (kDa)

6.39

Isoelectric Point (pI)

34.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_17	PF00332	41 - 359	3.7e-63	Glycosyl hydrolases family 17
X8	PF07983	380 - 449	1.3e-14	X8 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012546)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58090
fragaria_vesca	FvH4_4g00080 FvH4_4g00080 FvH4_4g00080 FvH4_4g00080
malus_domestica	MD04G1001100.v1.1
prunus_persica	Prupe.1G001100_v2.0.a1
pyrus_communis	pycom04g00170
rosa_laevigata	RLG00000010278
rosa_multiflora	Rmu_co8514003.1_g000001
rosa_roxburghii	Rroxscaffold_5G00332690
rosa_rugosa	Rorug03G0297000 Rorug03G0297100 Rorug03G0297100
rosa_samantha	Rh4AG000600 Rh4BG000500 Rh4CG000400 Rh4DG000300
rosa_wichuraiana	Rw4G000840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	668
AasI	GACNNNNNNGTC	1 cut(s)	173
AccB7I	CCANNNNNTGG	2 cut(s)	569, 925
AccBSI	CCGCTC	1 cut(s)	117
AciI	CCGC	2 cut(s)	117, 205
AclWI	GGATC	3 cut(s)	1004, 1387, 1428
AcoI	YGGCCR	1 cut(s)	851
AcsI	RAATTY	2 cut(s)	820, 1090
AcuI	CTGAAG	1 cut(s)	1143
AdeI	CACNNNGTG	1 cut(s)	1193
AfaI	GTAC	2 cut(s)	522, 1225
AflIII	ACRYGT	1 cut(s)	537
AgsI	TTSAA	5 cut(s)	552, 608, 901, 1025, 1350
AjiI	CACGTC	1 cut(s)	538
AjnI	CCWGG	3 cut(s)	952, 1012, 1461
AleI	CACNNNNGTG	1 cut(s)	1498
AluBI	AGCT	7 cut(s)	214, 435, 491, 1072, 1200, 1328, 1421
AluI	AGCT	7 cut(s)	214, 435, 491, 1072, 1200, 1328, 1421
Alw21I	GWGCWC	2 cut(s)	60, 1125
Alw26I	GTCTC	3 cut(s)	251, 355, 943
Alw44I	GTGCAC	1 cut(s)	1121
AlwI	GGATC	3 cut(s)	1004, 1387, 1428
AoxI	GGCC	4 cut(s)	30, 493, 851, 956
ApaLI	GTGCAC	1 cut(s)	1121
ApeKI	GCWGC	4 cut(s)	69, 72, 323, 1421
ApoI	RAATTY	2 cut(s)	820, 1090
AspS9I	GGNCC	2 cut(s)	795, 956
AsuC2I	CCSGG	1 cut(s)	1438
AsuHPI	GGTGA	7 cut(s)	115, 124, 526, 584, 637, 720, 1348
AvaII	GGWCC	1 cut(s)	795
BaeGI	GKGCMC	1 cut(s)	1125
BaeI	ACNNNNGTAYC	2 cut(s)	774, 807
BarI	GAAGNNNNNNTAC	2 cut(s)	165, 197
Bbv12I	GWGCWC	2 cut(s)	60, 1125
BbvI	GCAGC	4 cut(s)	81, 84, 310, 1408
BccI	CCATC	7 cut(s)	571, 710, 734, 843, 854, 1046, 1378
BceAI	ACGGC	1 cut(s)	211
BcgI	CGANNNNNNTGC	4 cut(s)	854, 888, 1260, 1294
BciT130I	CCWGG	3 cut(s)	954, 1014, 1463
BclI	TGATCA	1 cut(s)	1353
BcnI	CCSGG	1 cut(s)	1438
BcoDI	GTCTC	3 cut(s)	251, 355, 943
BfaI	CTAG	2 cut(s)	881, 1227
BfmI	CTRYAG	3 cut(s)	461, 800, 1422
BisI	GCNGC	4 cut(s)	70, 73, 324, 1422
BlsI	GCNGC	4 cut(s)	71, 74, 325, 1423
Bme1390I	CCNGG	4 cut(s)	954, 1014, 1438, 1463
Bme18I	GGWCC	1 cut(s)	795
BmgBI	CACGTC	1 cut(s)	538
BmgT120I	GGNCC	2 cut(s)	795, 956
BmiI	GGNNCC	4 cut(s)	127, 797, 940, 1084
BmrFI	CCNGG	4 cut(s)	954, 1014, 1438, 1463
BmrI	ACTGGG	2 cut(s)	142, 1042
BmsI	GCATC	6 cut(s)	155, 211, 955, 985, 1450, 1476
BmuI	ACTGGG	2 cut(s)	142, 1042
Bpu10I	CCTNAGC	1 cut(s)	497
BpuEI	CTTGAG	1 cut(s)	643
BpuMI	CCSGG	1 cut(s)	1438
BsaBI	GATNNNNATC	3 cut(s)	562, 969, 1400
BsaI	GGTCTC	1 cut(s)	943
BsaJI	CCNNGG	4 cut(s)	904, 953, 1078, 1231
BsaWI	WCCGGW	2 cut(s)	566, 867
BsaXI	ACNNNNNCTCC	6 cut(s)	350, 380, 668, 698, 1124, 1154
Bse1I	ACTGG	3 cut(s)	137, 472, 1037
Bse3DI	GCAATG	1 cut(s)	727
Bse8I	GATNNNNATC	3 cut(s)	562, 969, 1400
BseBI	CCWGG	3 cut(s)	954, 1014, 1463
BseDI	CCNNGG	4 cut(s)	904, 953, 1078, 1231
BseGI	GGATG	7 cut(s)	15, 24, 146, 563, 854, 1000, 1267
BseJI	GATNNNNATC	3 cut(s)	562, 969, 1400
BseMI	GCAATG	1 cut(s)	727
BseMII	CTCAG	5 cut(s)	86, 318, 454, 905, 1448
BseNI	ACTGG	3 cut(s)	137, 472, 1037
BseRI	GAGGAG	1 cut(s)	355
BseSI	GKGCMC	1 cut(s)	1125
BseXI	GCAGC	4 cut(s)	81, 84, 310, 1408
BsgI	GTGCAG	1 cut(s)	441
Bsh1285I	CGRYCG	1 cut(s)	868
BshFI	GGCC	4 cut(s)	32, 495, 853, 958
BsiEI	CGRYCG	1 cut(s)	868
BsiHKAI	GWGCWC	2 cut(s)	60, 1125
BsiSI	CCGG	4 cut(s)	178, 567, 868, 1437
BslFI	GGGAC	3 cut(s)	151, 901, 1255
BsmAI	GTCTC	3 cut(s)	251, 355, 943
BsmBI	CGTCTC	1 cut(s)	355
BsmFI	GGGAC	3 cut(s)	151, 901, 1255
BsmI	GAATGC	2 cut(s)	279, 877
BsnI	GGCC	4 cut(s)	32, 495, 853, 958
Bso31I	GGTCTC	1 cut(s)	943
Bsp1286I	GDGCHC	2 cut(s)	60, 1125
Bsp143I	GATC	7 cut(s)	381, 1009, 1264, 1353, 1379, 1401, 1433
BspACI	CCGC	2 cut(s)	117, 205
BspANI	GGCC	4 cut(s)	32, 495, 853, 958
BspCNI	CTCAG	5 cut(s)	87, 319, 453, 904, 1449
BspLI	GGNNCC	4 cut(s)	127, 797, 940, 1084
BspMAI	CTGCAG	3 cut(s)	465, 804, 1426
BspPI	GGATC	3 cut(s)	1004, 1387, 1428
BspTNI	GGTCTC	1 cut(s)	943
BsrBI	CCGCTC	1 cut(s)	117
BsrDI	GCAATG	1 cut(s)	727
BsrI	ACTGG	3 cut(s)	137, 472, 1037
BssECI	CCNNGG	4 cut(s)	904, 953, 1078, 1231
BssMI	GATC	7 cut(s)	381, 1009, 1264, 1353, 1379, 1401, 1433
BssT1I	CCWWGG	3 cut(s)	904, 1078, 1231
Bst2UI	CCWGG	3 cut(s)	954, 1014, 1463
Bst4CI	ACNGT	6 cut(s)	172, 245, 431, 520, 649, 1295
BstC8I	GCNNGC	4 cut(s)	297, 493, 1165, 1330
BstDEI	CTNAG	8 cut(s)	95, 252, 327, 440, 497, 891, 1190, 1457
BstF5I	GGATG	7 cut(s)	15, 24, 146, 563, 854, 1000, 1267
BstKTI	GATC	7 cut(s)	384, 1012, 1267, 1356, 1382, 1404, 1436
BstMAI	GTCTC	3 cut(s)	251, 355, 943
BstMBI	GATC	7 cut(s)	381, 1009, 1264, 1353, 1379, 1401, 1433
BstMCI	CGRYCG	1 cut(s)	868
BstMWI	GCNNNNNNNGC	3 cut(s)	211, 305, 1475
BstNI	CCWGG	3 cut(s)	954, 1014, 1463
BstSCI	CCNGG	4 cut(s)	952, 1012, 1436, 1461
BstSFI	CTRYAG	3 cut(s)	461, 800, 1422
BstSLI	GKGCMC	1 cut(s)	1125
BstV1I	GCAGC	4 cut(s)	81, 84, 310, 1408
BstX2I	RGATCY	1 cut(s)	1433
BstYI	RGATCY	1 cut(s)	1433
BsuRI	GGCC	4 cut(s)	32, 495, 853, 958
BtrI	CACGTC	1 cut(s)	538
BtsCI	GGATG	7 cut(s)	15, 24, 146, 563, 854, 1000, 1267
BtsI	GCAGTG	1 cut(s)	458
BtsIMutI	CAGTG	2 cut(s)	458, 1030
Cac8I	GCNNGC	4 cut(s)	297, 493, 1165, 1330
Cfr13I	GGNCC	2 cut(s)	795, 956
CseI	GACGC	2 cut(s)	304, 779
Csp6I	GTAC	2 cut(s)	521, 1224
CspCI	CAANNNNNGTGG	2 cut(s)	33, 68
CviAII	CATG	6 cut(s)	61, 271, 593, 601, 913, 1383
CviQI	GTAC	2 cut(s)	521, 1224
DdeI	CTNAG	8 cut(s)	95, 252, 327, 440, 497, 891, 1190, 1457
DpnI	GATC	7 cut(s)	383, 1011, 1266, 1355, 1381, 1403, 1435
DpnII	GATC	7 cut(s)	381, 1009, 1264, 1353, 1379, 1401, 1433
DraIII	CACNNNGTG	1 cut(s)	1193
DrdI	GACNNNNNNGTC	1 cut(s)	173
DseDI	GACNNNNNNGTC	1 cut(s)	173
EaeI	YGGCCR	1 cut(s)	851
Eco130I	CCWWGG	3 cut(s)	904, 1078, 1231
Eco31I	GGTCTC	1 cut(s)	943
Eco32I	GATATC	1 cut(s)	589
Eco47I	GGWCC	1 cut(s)	795
Eco57I	CTGAAG	1 cut(s)	1143
EcoRII	CCWGG	3 cut(s)	952, 1012, 1461
EcoRV	GATATC	1 cut(s)	589
EcoT14I	CCWWGG	3 cut(s)	904, 1078, 1231
ErhI	CCWWGG	3 cut(s)	904, 1078, 1231
Esp3I	CGTCTC	1 cut(s)	355
FaeI	CATG	6 cut(s)	64, 274, 596, 604, 916, 1386
FalI	AAGNNNNNCTT	4 cut(s)	480, 512, 797, 829
FaqI	GGGAC	3 cut(s)	151, 901, 1255
FatI	CATG	6 cut(s)	60, 270, 592, 600, 912, 1382
FauI	CCCGC	2 cut(s)	110, 198
FbaI	TGATCA	1 cut(s)	1353
Fnu4HI	GCNGC	4 cut(s)	70, 73, 324, 1422
FokI	GGATG	7 cut(s)	22, 31, 133, 550, 861, 1007, 1274
Fsp4HI	GCNGC	4 cut(s)	70, 73, 324, 1422
FspBI	CTAG	2 cut(s)	881, 1227
GluI	GCNGC	4 cut(s)	70, 73, 324, 1422
HaeIII	GGCC	4 cut(s)	32, 495, 853, 958
HapII	CCGG	4 cut(s)	178, 567, 868, 1437
HgaI	GACGC	2 cut(s)	304, 779
Hin1II	CATG	6 cut(s)	64, 274, 596, 604, 916, 1386
HincII	GTYRAC	1 cut(s)	1291
HindII	GTYRAC	1 cut(s)	1291
HindIII	AAGCTT	2 cut(s)	212, 1326
HinfI	GANTC	2 cut(s)	604, 683
HpaI	GTTAAC	1 cut(s)	1291
HpaII	CCGG	4 cut(s)	178, 567, 868, 1437
HphI	GGTGA	7 cut(s)	115, 124, 526, 584, 637, 720, 1348
Hpy166II	GTNNAC	5 cut(s)	652, 795, 1123, 1211, 1291
Hpy188I	TCNGA	5 cut(s)	443, 581, 894, 1379, 1458
Hpy188III	TCNNGA	3 cut(s)	379, 622, 1483
Hpy8I	GTNNAC	5 cut(s)	652, 795, 1123, 1211, 1291
HpyAV	CCTTC	4 cut(s)	460, 652, 718, 1167
HpyCH4III	ACNGT	6 cut(s)	172, 245, 431, 520, 649, 1295
HpyCH4IV	ACGT	2 cut(s)	348, 537
HpyF10VI	GCNNNNNNNGC	3 cut(s)	211, 305, 1475
HpyF3I	CTNAG	8 cut(s)	95, 252, 327, 440, 497, 891, 1190, 1457
HpySE526I	ACGT	2 cut(s)	348, 537
Hsp92II	CATG	6 cut(s)	64, 274, 596, 604, 916, 1386
Ksp22I	TGATCA	1 cut(s)	1353
KspAI	GTTAAC	1 cut(s)	1291
Kzo9I	GATC	7 cut(s)	381, 1009, 1264, 1353, 1379, 1401, 1433
LmnI	GCTCC	4 cut(s)	66, 125, 1132, 1418
Lsp1109I	GCAGC	4 cut(s)	81, 84, 310, 1408
LweI	GCATC	6 cut(s)	155, 211, 955, 985, 1450, 1476
MaeI	CTAG	2 cut(s)	881, 1227
MaeII	ACGT	2 cut(s)	348, 537
MaeIII	GTNAC	6 cut(s)	40, 514, 572, 626, 1235, 1295
MalI	GATC	7 cut(s)	383, 1011, 1266, 1355, 1381, 1403, 1435
MbiI	CCGCTC	1 cut(s)	117
MboI	GATC	7 cut(s)	381, 1009, 1264, 1353, 1379, 1401, 1433
MboII	GAAGA	1 cut(s)	817
MfeI	CAATTG	1 cut(s)	960
MflI	RGATCY	1 cut(s)	1433
MhlI	GDGCHC	2 cut(s)	60, 1125
MluCI	AATT	4 cut(s)	609, 820, 960, 1090
MlyI	GAGTC	1 cut(s)	677
MmeI	TCCRAC	5 cut(s)	379, 393, 711, 817, 1357
MseI	TTAA	3 cut(s)	210, 1074, 1290
MslI	CAYNNNNRTG	1 cut(s)	1498
MspA1I	CMGCKG	1 cut(s)	205
MspI	CCGG	4 cut(s)	178, 567, 868, 1437
MspR9I	CCNGG	4 cut(s)	954, 1014, 1438, 1463
MunI	CAATTG	1 cut(s)	960
Mva1269I	GAATGC	2 cut(s)	279, 877
MvaI	CCWGG	3 cut(s)	954, 1014, 1463
MwoI	GCNNNNNNNGC	3 cut(s)	211, 305, 1475
NciI	CCSGG	1 cut(s)	1438
NdeII	GATC	7 cut(s)	381, 1009, 1264, 1353, 1379, 1401, 1433
NlaIII	CATG	6 cut(s)	64, 274, 596, 604, 916, 1386
NlaIV	GGNNCC	4 cut(s)	127, 797, 940, 1084
NmuCI	GTSAC	3 cut(s)	514, 572, 626
OliI	CACNNNNGTG	1 cut(s)	1498
PcsI	WCGNNNNNNNCGW	1 cut(s)	312
PctI	GAATGC	2 cut(s)	279, 877
PfeI	GAWTC	1 cut(s)	604
PflMI	CCANNNNNTGG	2 cut(s)	569, 925
PfoI	TCCNGGA	1 cut(s)	1012
PkrI	GCNGC	4 cut(s)	71, 74, 325, 1423
PleI	GAGTC	1 cut(s)	677
PpsI	GAGTC	1 cut(s)	677
PsiI	TTATAA	1 cut(s)	668
Psp6I	CCWGG	3 cut(s)	952, 1012, 1461
PspGI	CCWGG	3 cut(s)	952, 1012, 1461
PspN4I	GGNNCC	4 cut(s)	127, 797, 940, 1084
PspPI	GGNCC	2 cut(s)	795, 956
PstI	CTGCAG	3 cut(s)	465, 804, 1426
PsuI	RGATCY	1 cut(s)	1433
RsaI	GTAC	2 cut(s)	522, 1225
RsaNI	GTAC	2 cut(s)	521, 1224
RseI	CAYNNNNRTG	1 cut(s)	1498
SaqAI	TTAA	3 cut(s)	210, 1074, 1290
SatI	GCNGC	4 cut(s)	70, 73, 324, 1422
Sau3AI	GATC	7 cut(s)	381, 1009, 1264, 1353, 1379, 1401, 1433
Sau96I	GGNCC	2 cut(s)	795, 956
SchI	GAGTC	1 cut(s)	677
ScrFI	CCNGG	4 cut(s)	954, 1014, 1438, 1463
SduI	GDGCHC	2 cut(s)	60, 1125
SfaNI	GCATC	6 cut(s)	155, 211, 955, 985, 1450, 1476
SfcI	CTRYAG	3 cut(s)	461, 800, 1422
SinI	GGWCC	1 cut(s)	795
SmiMI	CAYNNNNRTG	1 cut(s)	1498
SmlI	CTYRAG	1 cut(s)	622
SmoI	CTYRAG	1 cut(s)	622
Sse9I	AATT	4 cut(s)	609, 820, 960, 1090
SsiI	CCGC	2 cut(s)	117, 205
SspI	AATATT	1 cut(s)	1041
SspMI	CTAG	2 cut(s)	881, 1227
StyD4I	CCNGG	4 cut(s)	952, 1012, 1436, 1461
StyI	CCWWGG	3 cut(s)	904, 1078, 1231
TaaI	ACNGT	6 cut(s)	172, 245, 431, 520, 649, 1295
TaiI	ACGT	2 cut(s)	351, 540
TaqI	TCGA	3 cut(s)	219, 1267, 1404
TasI	AATT	4 cut(s)	609, 820, 960, 1090
TatI	WGTACW	1 cut(s)	1223
TfiI	GAWTC	1 cut(s)	604
Tru1I	TTAA	3 cut(s)	210, 1074, 1290
Tru9I	TTAA	3 cut(s)	210, 1074, 1290
TscAI	CASTG	2 cut(s)	465, 1037
TseFI	GTSAC	3 cut(s)	514, 572, 626
TseI	GCWGC	4 cut(s)	69, 72, 323, 1421
Tsp45I	GTSAC	3 cut(s)	514, 572, 626
TspDTI	ATGAA	3 cut(s)	901, 1166, 1338
TspGWI	ACGGA	1 cut(s)	1431
TspRI	CASTG	2 cut(s)	465, 1037
Van91I	CCANNNNNTGG	2 cut(s)	569, 925
VneI	GTGCAC	1 cut(s)	1121
VpaK11BI	GGWCC	1 cut(s)	795
XapI	RAATTY	2 cut(s)	820, 1090
XcmI	CCANNNNNNNNNTGG	2 cut(s)	926, 1076
XspI	CTAG	2 cut(s)	881, 1227

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.