MD04G1013500.v1.1

Belongs to the glycosyltransferase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011248
Physical Location & Seq
Reverse (-)
1538915 .. 1544375
5461 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1013500.v1.1.491

Sequence Viewer

Length: 3198 bp
ATGGCCGGAAACGAGTGGATTAATGGGTACTTGGAGGCGATACTGGACAGCGGCGCCTCCGCCATTGAGGAGCAGAAGCCGGTGCCGGAGAATCTGAGAGATAGAGGACATTTCAATCCGACCAAGTACTTCGTGGAGGAGGTGGTGACGGGGGTCGATGAGTCGGACCTTTACCGGACGTGGATCAAGGTTGTTGCGACCCGCAACACGCGGGAACGGGGCTCCAGGCTCGAGAATATGTGCTGGCGCATTTGGCACCTTACCCGTAAGAAGAAACAGTTGGAGGTGGAGGAACATCAGCGGTTGGCAAACCGCAGATGGGAGCGGGAACAAGGACGTAGGGATGCAACTGAAGACATGTCGGAGGACTTGTCCGAAGGAGAGAAAGGGGATGGTTTGGGGGAGATGCAACTAGGTGATACGCCAAGGAAAAGGTTCCAGCGTAATATTTCGAACTTGGAAGTATGGTCAGACGATAAGAAGGAAAAGAAGCTTTACATTGTTCTTATCAGTTTGCATGGTTTGGTCCGGGGAGAAAACATGGAGCTCGGTCGAGACTCTGATACAGGTGGACAGGTCAAATATGTGGTAGAGCTTTCCCGAGCACTTGCGAGGATGCCTGGGGTTTATAGGGTAGATCTCTTTACGCGCCAAGTGTCATGTCCTGATGTTGATTGGAGCTATGGTGAGCCAACAGAGATGCTAACTGCAGGTCCTGAAGATGGTGATGGTGATCTGGGAGAAAGCAGTGGAGCGTATATTATCAGAATTCCTTTCGGTCCTCGTGATCAATACCTCAGCAAAGAAGTACTCTGGCCATATATTCAAGAATTTGTAGATGGTGCTCTAGCTCACATTCTTAACATGTCCAAAGTTTTGGGAGAACAGATTGGTAAAGGACAGCCTGTCTGGCCGTATGTAATTCATGGCCACTACGCAGATGCAGGGGATAGTGCTGCTCTTCTTTCTGGTGCTTTGAATGTCCCAATGGTTTTAACTGGGCACTCACTTGGAAGAAACAAGCTTGAACAGCTTCTTAAGCAGGGACGCCAATCAAAGGAGGACATCAATTCTACATATAAAATTATGAGGAGGATTGAAGCAGAAGAACTTTCCCTTGATGCTGCAGAACTAGTTATTACGAGTACAAAACAAGAGAGCGAAGAGCAATGGGGGCTTTACGATGGGTTTGATGTCAAGCTTGAGAAAGTTTTGCGTGCTCGTGCTAGGCGAGGAGTAAATTGCCATGGCCGTTACATGCCAAGGATGGTGGTTATACCTCCTGGCATGGACTTCAGTAATGTTGTGGTTCAAGAAGATACACCTGAGGTTGATGGCGAACTTAACCAACTTGGTGGTACTGATGGGTCTTCCCCGAAAGCAATTCCAACGATATGGTCAGAAGTGATGCGGTTCCTTGCAAATCCTCACAAGCCAATGATCTTGGCTTTGTCAAGACCAGATCCGAAGAAGAACTTAACCACTCTTTTAAAAGCCTTTGGGGAATGCGGTCCTCTTAGAGAGCTCGCTAATCTTACACTGATAATGGGGAATAGGGACTGCATAGATGAGATGTCTACCGGAAATGCTAGTGTTCTTACGACAGTTTTGAAACTAATTGATAAGTATGACCTCTATGGGCAAGTGGCATATCCAAAGCATCACAGGCAATCAGATGTTCCAGATATATACCGTCTCACGGCAAAAACAAAGGGAGTTTTCATCAATCCAGCATTAGTGGAGCCTTTTGGGCTAACCTTGATTGAGGCAGCAGCACATGGGCTTCCAATGGTAGCTACAAAAAACGGTGGACCGGTTGATATACATCGGGCGCTGAATAATGGTTTGCTTGTGGACCCTCATGATCAGCAGTCGATTGCTGGTGCACTTCTTAAGTTGTTATCGGAGAAGAATTTATGGAGTGAATGTAGAAAGAATGGCTGGAAGAATATACACCTCTACTCATGGCCTGAACATTGCCGGACTTACTTGACTAGGGTTGCAGGCTGCCGCATGAGACATCCACAATGGCAAACTGACACCCCAGAGGATGATATGGCTGCTGATGAGTCTCTCAATGATTCTCTTAAGGATGTGCAAGATATGTCTCTTAGGCTCTCAGTTGATGGGGATAAAGCTTCATTGAACGGATCTTTTGATGTGACTGCTGCCGCTGGTGACCACGATGTGCAAGATCAAGTGAAACGTGTCCTAAGCAAGATCAAGAAACCAGATTCCGGTCCAAAAGATCAAGATGGCGGAAATAAGCCGCTGGATAATGTGTCAAGTAAATATCCCATGTTAAGGAGGAGACGGAAATTGATTGTTATAGCGCTTGATTGCTACGAAAGCTCTGGAGATCCTAAAAAGAAGATGATTCAGGTAGTTCAAGAAATATTTAAGGCTGTCAGATTAGACTCTCAAAGTGCTAGGGTCACAGGGTTTGCTCTATTAACGGCTATGCCAATGTTAGAAACTGTAGAGTTTTTGGCATCAGGGAAGATACAGGCAAACGAATTTGACGCGTTGGTTTGTAGCAGTGGAAGTGAAGTTTACTACCCTGGTACTTATACAGAAGGCGGCGGAAGACTTTCCCCAGATCCAGATTATGCATCACATATTGACTACCGTTGGGGTTGTGAGGGTCTAAAGAAAACAATTTTGAAGCTGTTGAATGCCCCTGAAGGCGAAGGAAATTCTGCTTCTTCCAGCCACATTCAAGAAGACTTAAAATCAAGCAACGCACATTGCATCTCATTCTTCATAAAGGATCCTAGTAAGGCAAGGAAAGTTGATGATTTGAGGCAGAAACTCAGGATGCGGGGACTGCGCTGCCATCCAATGTACTCCAGGAGCTCAACGAGAATGCAAATTGTTCCTCTCCTTGCTTCTCGAGCGCAGGCACTCAGGTACCTTTTTGTTCGTTGGAGATTGAATGTCGCCAACATGTACGTGTTCCTTGGAGAAAGTGGAGACACCGATTATGAAGAAATGATATCTGGAACTCATAAGACAATAATCATGAAAGGGGTGGTGGCAAAGGGTTCGGAAGAGCTGCTAAGAACATCAGGAAGCTATCTCAGAGAAGACATTGTTCCTCACGAGAGCCCTTTGGTCGCCTACGTAAGCGAGGAAGCAAAAGCTGATGAGATTGCTAATGCTCTGAAGCAAGTCTCTAAGTCTGCTGCTGGAATGTGA

Protein Analysis

1066

Amino Acids

119.18

Weight (kDa)

6.15

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_trans_4_4 PF13579 190 - 388 5.4e-06 Glycosyl transferase 4-like domain
GT-B_Sucrose_synth PF00862 250 - 432 2.7e-08 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 474 - 647 2.9e-22 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 479 - 635 1.7e-13 Glycosyl transferases group 1
S6PP PF05116 839 - 1007 3.8e-15 Sucrose-6F-phosphate phosphohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011248)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04920
fragaria_vesca FvH4_4g13520 FvH4_4g13520 FvH4_4g13520
malus_domestica MD04G1013500.v1.1
prunus_persica Prupe.1G159700_v2.0.a1 Prupe.1G159700_v2.0.a1 Prupe.1G159700_v2.0.a1
pyrus_communis pycom04g01050
rosa_chinensis RchiOBHm_Chr4g0414151
rosa_laevigata RLG00000008166
rosa_multiflora Rmu_sc0001063.1_g000015
rosa_roxburghii Rroxscaffold_5G00358160
rosa_rugosa Rorug04G0126600
rosa_samantha Rh4AG184700 Rh4BG184100 Rh4CG196400 Rh4DG180000
rosa_wichuraiana Rw0G005040 Rw4G015730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 701
Acc65I GGTACC 1 cut(s) 2910
AccB1I GGYRCC 4 cut(s) 53, 82, 255, 2910
AccBSI CCGCTC 1 cut(s) 325
AccI GTMKAC 1 cut(s) 1577
AccII CGCG 3 cut(s) 211, 649, 2525
AclWI GGATC 7 cut(s) 191, 1457, 2158, 2354, 2594, 2765, 2778
AcoI YGGCCR 5 cut(s) 3, 815, 911, 928, 1249
AcsI RAATTY 5 cut(s) 768, 830, 1912, 2516, 2695
AcuI CTGAAG 5 cut(s) 372, 738, 1279, 2703, 3185
AcyI GRCGYC 2 cut(s) 54, 1048
AdeI CACNNNGTG 1 cut(s) 2188
AfaI GTAC 9 cut(s) 29, 128, 810, 1147, 1360, 2566, 2846, 2912, 2951
AfeI AGCGCT 1 cut(s) 2334
AfiI CCNNNNNNNGG 6 cut(s) 319, 722, 1057, 1699, 2237, 2304
AflII CTTAAG 3 cut(s) 1037, 1892, 2087
AflIII ACRYGT 6 cut(s) 357, 864, 2206, 2523, 2946, 2952
AgeI ACCGGT 1 cut(s) 1813
AhdI GACNNNNNGTC 1 cut(s) 905
AhlI ACTAGT 1 cut(s) 1132
AjiI CACGTC 1 cut(s) 180
AjnI CCWGG 5 cut(s) 224, 619, 1282, 2560, 2849
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
Alw21I GWGCWC 7 cut(s) 549, 607, 847, 1222, 1527, 1888, 2858
Alw26I GTCTC 8 cut(s) 549, 1700, 2011, 2076, 2112, 2305, 2967, 3178
Alw44I GTGCAC 1 cut(s) 1884
AlwI GGATC 7 cut(s) 191, 1457, 2158, 2354, 2594, 2765, 2778
AlwNI CAGNNNCTG 1 cut(s) 716
Ama87I CYCGRG 3 cut(s) 230, 600, 2892
Aor51HI AGCGCT 1 cut(s) 2334
AoxI GGCC 6 cut(s) 3, 815, 911, 928, 1249, 1967
ApaLI GTGCAC 1 cut(s) 1884
ApoI RAATTY 5 cut(s) 768, 830, 1912, 2516, 2695
AseI ATTAAT 1 cut(s) 21
AsiGI ACCGGT 1 cut(s) 1813
Asp700I GAANNNNTTC 3 cut(s) 434, 1032, 2590
Asp718I GGTACC 1 cut(s) 2910
AspLEI GCGC 7 cut(s) 56, 249, 651, 1834, 2335, 2832, 2899
AspS9I GGNCC 8 cut(s) 166, 526, 713, 779, 1511, 1811, 1855, 2240
AsuC2I CCSGG 1 cut(s) 530
AsuHPI GGTGA 6 cut(s) 157, 428, 698, 737, 743, 2189
AsuII TTCGAA 1 cut(s) 452
AvaI CYCGRG 3 cut(s) 230, 600, 2892
AvaII GGWCC 8 cut(s) 166, 526, 713, 779, 1511, 1811, 1855, 2240
AxyI CCTNAGG 1 cut(s) 1326
BaeGI GKGCMC 2 cut(s) 1005, 1888
BaeI ACNNNNGTAYC 2 cut(s) 2894, 2927
BalI TGGCCA 2 cut(s) 817, 930
BamHI GGATCC 1 cut(s) 2770
BanI GGYRCC 4 cut(s) 53, 82, 255, 2910
BanII GRGCYC 5 cut(s) 224, 549, 1527, 2858, 3110
BauI CACGAG 3 cut(s) 783, 1221, 3101
BbsI GAAGAC 5 cut(s) 360, 1362, 2593, 2730, 3093
Bbv12I GWGCWC 7 cut(s) 549, 607, 847, 1222, 1527, 1888, 2858
BbvCI CCTCAGC 1 cut(s) 797
BceAI ACGGC 4 cut(s) 898, 1236, 1716, 2472
BcgI CGANNNNNNTGC 2 cut(s) 3027, 3061
BciT130I CCWGG 5 cut(s) 226, 621, 1284, 2562, 2851
BclI TGATCA 2 cut(s) 787, 1864
BcnI CCSGG 1 cut(s) 530
BcoDI GTCTC 8 cut(s) 549, 1700, 2011, 2076, 2112, 2305, 2967, 3178
BcuI ACTAGT 1 cut(s) 1132
BfaI CTAG 8 cut(s) 413, 848, 1133, 1227, 1590, 1995, 2430, 2775
BfmI CTRYAG 3 cut(s) 708, 1125, 2478
BfoI RGCGCY 3 cut(s) 57, 1835, 2336
BfrI CTTAAG 3 cut(s) 1037, 1892, 2087
BfuAI ACCTGC 1 cut(s) 701
BglI GCCNNNNNGGC 2 cut(s) 910, 1750
BglII AGATCT 1 cut(s) 637
BmcAI AGTACT 2 cut(s) 128, 810
Bme1390I CCNGG 6 cut(s) 226, 530, 621, 1284, 2562, 2851
Bme18I GGWCC 8 cut(s) 166, 526, 713, 779, 1511, 1811, 1855, 2240
BmeRI GACNNNNNGTC 1 cut(s) 905
BmeT110I CYCGRG 3 cut(s) 230, 600, 2892
BmgBI CACGTC 1 cut(s) 180
BmgT120I GGNCC 8 cut(s) 166, 526, 713, 779, 1511, 1811, 1855, 2240
BmrFI CCNGG 6 cut(s) 226, 530, 621, 1284, 2562, 2851
BmrI ACTGGG 1 cut(s) 1008
BmuI ACTGGG 1 cut(s) 1008
BoxI GACNNNNGTC 1 cut(s) 152
BpiI GAAGAC 5 cut(s) 360, 1362, 2593, 2730, 3093
BpmI CTGGAG 3 cut(s) 208, 2376, 2833
Bpu10I CCTNAGC 2 cut(s) 797, 2213
Bpu14I TTCGAA 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 1223
BpuMI CCSGG 1 cut(s) 530
BsaAI YACGTR 2 cut(s) 2953, 3124
BsaBI GATNNNNATC 2 cut(s) 732, 1824
BsaHI GRCGYC 2 cut(s) 54, 1048
BsaJI CCNNGG 7 cut(s) 425, 529, 620, 1246, 1262, 2560, 2959
BsaWI WCCGGW 4 cut(s) 174, 1580, 1813, 2237
BsaXI ACNNNNNCTCC 6 cut(s) 356, 386, 670, 700, 1912, 1942
Bsc4I CCNNNNNNNGG 6 cut(s) 319, 722, 1057, 1699, 2237, 2304
Bse118I RCCGGY 2 cut(s) 79, 1813
Bse1I ACTGG 2 cut(s) 48, 1003
Bse21I CCTNAGG 1 cut(s) 1326
Bse3DI GCAATG 3 cut(s) 1175, 1975, 2746
Bse8I GATNNNNATC 2 cut(s) 732, 1824
BseBI CCWGG 5 cut(s) 226, 621, 1284, 2562, 2851
BseDI CCNNGG 7 cut(s) 425, 529, 620, 1246, 1262, 2560, 2959
BseGI GGATG 9 cut(s) 349, 397, 621, 1272, 2020, 2056, 2098, 2823, 2836
BseJI GATNNNNATC 2 cut(s) 732, 1824
BseLI CCNNNNNNNGG 6 cut(s) 319, 722, 1057, 1699, 2237, 2304
BseMI GCAATG 3 cut(s) 1175, 1975, 2746
BseMII CTCAG 7 cut(s) 86, 811, 1317, 2133, 2827, 2920, 3094
BseNI ACTGG 2 cut(s) 48, 1003
BseRI GAGGAG 5 cut(s) 83, 152, 1105, 1248, 2323
BseSI GKGCMC 2 cut(s) 1005, 1888
Bsh1236I CGCG 3 cut(s) 211, 649, 2525
Bsh1285I CGRYCG 1 cut(s) 553
BshFI GGCC 6 cut(s) 5, 817, 913, 930, 1251, 1969
BshNI GGYRCC 4 cut(s) 53, 82, 255, 2910
BshTI ACCGGT 1 cut(s) 1813
BsiEI CGRYCG 1 cut(s) 553
BsiHKAI GWGCWC 7 cut(s) 549, 607, 847, 1222, 1527, 1888, 2858
BsiHKCI CYCGRG 3 cut(s) 230, 600, 2892
BsiSI CCGG 9 cut(s) 6, 80, 86, 175, 529, 1581, 1814, 1981, 2238
BslFI GGGAC 4 cut(s) 968, 1059, 1571, 2838
BslI CCNNNNNNNGG 6 cut(s) 319, 722, 1057, 1699, 2237, 2304
BsmAI GTCTC 8 cut(s) 549, 1700, 2011, 2076, 2112, 2305, 2967, 3178
BsmBI CGTCTC 2 cut(s) 1700, 2305
BsmFI GGGAC 4 cut(s) 968, 1059, 1571, 2838
BsmI GAATGC 3 cut(s) 1511, 2680, 2871
BsnI GGCC 6 cut(s) 5, 817, 913, 930, 1251, 1969
BsoBI CYCGRG 3 cut(s) 230, 600, 2892
Bsp119I TTCGAA 1 cut(s) 452
Bsp19I CCATGG 1 cut(s) 1246
BspANI GGCC 6 cut(s) 5, 817, 913, 930, 1251, 1969
BspCNI CTCAG 7 cut(s) 87, 810, 1318, 2132, 2826, 2919, 3093
BspFNI CGCG 3 cut(s) 211, 649, 2525
BspHI TCATGA 2 cut(s) 1861, 3021
BspMAI CTGCAG 2 cut(s) 712, 1129
BspMI ACCTGC 1 cut(s) 701
BspPI GGATC 7 cut(s) 191, 1457, 2158, 2354, 2594, 2765, 2778
BspQI GCTCTTC 3 cut(s) 966, 1158, 3045
BspT104I TTCGAA 1 cut(s) 452
BspT107I GGYRCC 4 cut(s) 53, 82, 255, 2910
BspTI CTTAAG 3 cut(s) 1037, 1892, 2087
BsrBI CCGCTC 1 cut(s) 325
BsrDI GCAATG 3 cut(s) 1175, 1975, 2746
BsrFI RCCGGY 2 cut(s) 79, 1813
BsrI ACTGG 2 cut(s) 48, 1003
BssAI RCCGGY 2 cut(s) 79, 1813
BssECI CCNNGG 7 cut(s) 425, 529, 620, 1246, 1262, 2560, 2959
BssNI GRCGYC 2 cut(s) 54, 1048
BssSI CACGAG 3 cut(s) 783, 1221, 3101
BssT1I CCWWGG 4 cut(s) 425, 1246, 1262, 2959
Bst2BI CACGAG 3 cut(s) 783, 1221, 3101
Bst2UI CCWGG 5 cut(s) 226, 621, 1284, 2562, 2851
Bst4CI ACNGT 6 cut(s) 279, 1606, 1694, 1808, 2479, 2630
Bst6I CTCTTC 3 cut(s) 966, 1158, 3045
BstACI GRCGYC 2 cut(s) 54, 1048
BstAFI CTTAAG 3 cut(s) 1037, 1892, 2087
BstBAI YACGTR 2 cut(s) 2953, 3124
BstBI TTCGAA 1 cut(s) 452
BstC8I GCNNGC 5 cut(s) 245, 1218, 1527, 2005, 2901
BstDSI CCRYGG 1 cut(s) 1246
BstEII GGTNACC 1 cut(s) 2177
BstF5I GGATG 9 cut(s) 349, 397, 621, 1272, 2020, 2056, 2098, 2823, 2836
BstFNI CGCG 3 cut(s) 211, 649, 2525
BstH2I RGCGCY 3 cut(s) 57, 1835, 2336
BstHHI GCGC 7 cut(s) 56, 249, 651, 1834, 2335, 2832, 2899
BstMAI GTCTC 8 cut(s) 549, 1700, 2011, 2076, 2112, 2305, 2967, 3178
BstMCI CGRYCG 1 cut(s) 553
BstNI CCWGG 5 cut(s) 226, 621, 1284, 2562, 2851
BstNSI RCATGY 4 cut(s) 361, 868, 1261, 2950
BstPAI GACNNNNGTC 1 cut(s) 152
BstPI GGTNACC 1 cut(s) 2177
BstSCI CCNGG 6 cut(s) 224, 528, 619, 1282, 2560, 2849
BstSFI CTRYAG 3 cut(s) 708, 1125, 2478
BstSLI GKGCMC 2 cut(s) 1005, 1888
BstSNI TACGTA 1 cut(s) 3124
BstUI CGCG 3 cut(s) 211, 649, 2525
BstV2I GAAGAC 5 cut(s) 360, 1362, 2593, 2730, 3093
BstX2I RGATCY 6 cut(s) 637, 1462, 2150, 2359, 2599, 2770
BstXI CCANNNNNNTGG 3 cut(s) 877, 1355, 1395
BstYI RGATCY 6 cut(s) 637, 1462, 2150, 2359, 2599, 2770
Bsu36I CCTNAGG 1 cut(s) 1326
BsuRI GGCC 6 cut(s) 5, 817, 913, 930, 1251, 1969
BtgI CCRYGG 1 cut(s) 1246
BtrI CACGTC 1 cut(s) 180
BtsCI GGATG 9 cut(s) 349, 397, 621, 1272, 2020, 2056, 2098, 2823, 2836
BtsI GCAGTG 2 cut(s) 754, 2545
BtsIMutI CAGTG 3 cut(s) 754, 1538, 2545
BveI ACCTGC 1 cut(s) 701
Cac8I GCNNGC 5 cut(s) 245, 1218, 1527, 2005, 2901
CaiI CAGNNNCTG 1 cut(s) 716
CciI TCATGA 2 cut(s) 1861, 3021
CfoI GCGC 7 cut(s) 56, 249, 651, 1834, 2335, 2832, 2899
Cfr10I RCCGGY 2 cut(s) 79, 1813
Cfr13I GGNCC 8 cut(s) 166, 526, 713, 779, 1511, 1811, 1855, 2240
CseI GACGC 2 cut(s) 1056, 2531
Csp6I GTAC 9 cut(s) 28, 127, 809, 1146, 1359, 2565, 2845, 2911, 2950
CspAI ACCGGT 1 cut(s) 1813
CspCI CAANNNNNGTGG 2 cut(s) 1251, 1286
CviQI GTAC 9 cut(s) 28, 127, 809, 1146, 1359, 2565, 2845, 2911, 2950
DinI GGCGCC 1 cut(s) 55
DraI TTTAAA 1 cut(s) 1491
DraIII CACNNNGTG 1 cut(s) 2188
DriI GACNNNNNGTC 1 cut(s) 905
EaeI YGGCCR 5 cut(s) 3, 815, 911, 928, 1249
Eam1104I CTCTTC 3 cut(s) 966, 1158, 3045
Eam1105I GACNNNNNGTC 1 cut(s) 905
EarI CTCTTC 3 cut(s) 966, 1158, 3045
EciI GGCGGA 3 cut(s) 49, 2274, 2598
Ecl136II GAGCTC 3 cut(s) 547, 1525, 2856
Eco105I TACGTA 1 cut(s) 3124
Eco130I CCWWGG 4 cut(s) 425, 1246, 1262, 2959
Eco24I GRGCYC 5 cut(s) 224, 549, 1527, 2858, 3110
Eco32I GATATC 1 cut(s) 2997
Eco47I GGWCC 8 cut(s) 166, 526, 713, 779, 1511, 1811, 1855, 2240
Eco47III AGCGCT 1 cut(s) 2334
Eco53kI GAGCTC 3 cut(s) 547, 1525, 2856
Eco57I CTGAAG 5 cut(s) 372, 738, 1279, 2703, 3185
Eco81I CCTNAGG 1 cut(s) 1326
Eco88I CYCGRG 3 cut(s) 230, 600, 2892
Eco91I GGTNACC 1 cut(s) 2177
EcoICRI GAGCTC 3 cut(s) 547, 1525, 2856
EcoO109I RGGNCCY 1 cut(s) 713
EcoO65I GGTNACC 1 cut(s) 2177
EcoRI GAATTC 1 cut(s) 768
EcoRII CCWGG 5 cut(s) 224, 619, 1282, 2560, 2849
EcoRV GATATC 1 cut(s) 2997
EcoT14I CCWWGG 4 cut(s) 425, 1246, 1262, 2959
EcoT22I ATGCAT 1 cut(s) 2614
EcoT38I GRGCYC 5 cut(s) 224, 549, 1527, 2858, 3110
EgeI GGCGCC 1 cut(s) 55
EheI GGCGCC 1 cut(s) 55
ErhI CCWWGG 4 cut(s) 425, 1246, 1262, 2959
Esp3I CGTCTC 2 cut(s) 1700, 2305
FalI AAGNNNNNCTT 2 cut(s) 1460, 1492
FaqI GGGAC 4 cut(s) 968, 1059, 1571, 2838
FauI CCCGC 4 cut(s) 204, 209, 318, 2814
FbaI TGATCA 2 cut(s) 787, 1864
FblI GTMKAC 1 cut(s) 1577
FokI GGATG 9 cut(s) 356, 404, 628, 1279, 2007, 2063, 2105, 2823, 2830
FriOI GRGCYC 5 cut(s) 224, 549, 1527, 2858, 3110
FspBI CTAG 8 cut(s) 413, 848, 1133, 1227, 1590, 1995, 2430, 2775
GlaI GCGC 7 cut(s) 55, 248, 650, 1833, 2334, 2831, 2898
GsuI CTGGAG 3 cut(s) 208, 2376, 2833
HaeII RGCGCY 3 cut(s) 57, 1835, 2336
HaeIII GGCC 6 cut(s) 5, 817, 913, 930, 1251, 1969
HapII CCGG 9 cut(s) 6, 80, 86, 175, 529, 1581, 1814, 1981, 2238
HgaI GACGC 2 cut(s) 1056, 2531
HhaI GCGC 7 cut(s) 56, 249, 651, 1834, 2335, 2832, 2899
Hin1I GRCGYC 2 cut(s) 54, 1048
Hin6I GCGC 7 cut(s) 54, 247, 649, 1832, 2333, 2830, 2897
HinP1I GCGC 7 cut(s) 54, 247, 649, 1832, 2333, 2830, 2897
HindIII AAGCTT 4 cut(s) 491, 1022, 1199, 2136
HinfI GANTC 8 cut(s) 91, 161, 557, 2069, 2081, 2234, 2377, 2417
HpaII CCGG 9 cut(s) 6, 80, 86, 175, 529, 1581, 1814, 1981, 2238
HphI GGTGA 6 cut(s) 157, 428, 698, 737, 743, 2189
Hpy166II GTNNAC 6 cut(s) 572, 1578, 1811, 1855, 1886, 2554
Hpy8I GTNNAC 6 cut(s) 572, 1578, 1811, 1855, 1886, 2554
HpyAV CCTTC 5 cut(s) 371, 475, 2570, 2678, 2684
HpyCH4III ACNGT 6 cut(s) 279, 1606, 1694, 1808, 2479, 2630
HpyCH4IV ACGT 5 cut(s) 179, 337, 2206, 2952, 3123
HpySE526I ACGT 5 cut(s) 179, 337, 2206, 2952, 3123
Hsp92I GRCGYC 2 cut(s) 54, 1048
HspAI GCGC 7 cut(s) 54, 247, 649, 1832, 2333, 2830, 2897
KasI GGCGCC 1 cut(s) 53
KpnI GGTACC 1 cut(s) 2914
Ksp22I TGATCA 2 cut(s) 787, 1864
LguI GCTCTTC 3 cut(s) 966, 1158, 3045
LmnI GCTCC 8 cut(s) 70, 227, 322, 544, 678, 752, 1741, 2853
MaeI CTAG 8 cut(s) 413, 848, 1133, 1227, 1590, 1995, 2430, 2775
MaeII ACGT 5 cut(s) 179, 337, 2206, 2952, 3123
MaeIII GTNAC 5 cut(s) 145, 1253, 2161, 2177, 2434
MbiI CCGCTC 1 cut(s) 325
MflI RGATCY 6 cut(s) 637, 1462, 2150, 2359, 2599, 2770
MlsI TGGCCA 2 cut(s) 817, 930
MluI ACGCGT 1 cut(s) 2523
MluNI TGGCCA 2 cut(s) 817, 930
Mly113I GGCGCC 1 cut(s) 54
MlyI GAGTC 4 cut(s) 170, 551, 2078, 2411
MmeI TCCRAC 6 cut(s) 143, 144, 261, 342, 1412, 2906
Mox20I TGGCCA 2 cut(s) 817, 930
Mph1103I ATGCAT 1 cut(s) 2614
MroXI GAANNNNTTC 3 cut(s) 434, 1032, 2590
MscI TGGCCA 2 cut(s) 817, 930
MslI CAYNNNNRTG 1 cut(s) 2951
Msp20I TGGCCA 2 cut(s) 817, 930
MspA1I CMGCKG 4 cut(s) 51, 301, 2174, 2272
MspCI CTTAAG 3 cut(s) 1037, 1892, 2087
MspI CCGG 9 cut(s) 6, 80, 86, 175, 529, 1581, 1814, 1981, 2238
MspR9I CCNGG 6 cut(s) 226, 530, 621, 1284, 2562, 2851
Mva1269I GAATGC 3 cut(s) 1511, 2680, 2871
MvaI CCWGG 5 cut(s) 226, 621, 1284, 2562, 2851
MvnI CGCG 3 cut(s) 211, 649, 2525
NarI GGCGCC 1 cut(s) 54
NciI CCSGG 1 cut(s) 530
NcoI CCATGG 1 cut(s) 1246
NmuCI GTSAC 4 cut(s) 145, 2161, 2177, 2434
NsiI ATGCAT 1 cut(s) 2614
NspI RCATGY 4 cut(s) 361, 868, 1261, 2950
NspV TTCGAA 1 cut(s) 452
PaeR7I CTCGAG 2 cut(s) 230, 2892
PagI TCATGA 2 cut(s) 1861, 3021
PciI ACATGT 3 cut(s) 357, 864, 2946
PciSI GCTCTTC 3 cut(s) 966, 1158, 3045
PcsI WCGNNNNNNNCGW 1 cut(s) 1228
PctI GAATGC 3 cut(s) 1511, 2680, 2871
PdmI GAANNNNTTC 3 cut(s) 434, 1032, 2590
PfeI GAWTC 4 cut(s) 91, 2081, 2234, 2377
PfoI TCCNGGA 1 cut(s) 2849
PinAI ACCGGT 1 cut(s) 1813
PleI GAGTC 4 cut(s) 169, 551, 2077, 2411
PluTI GGCGCC 1 cut(s) 57
PpsI GAGTC 4 cut(s) 169, 551, 2077, 2411
Ppu21I YACGTR 2 cut(s) 2953, 3124
PpuMI RGGWCCY 1 cut(s) 713
PscI ACATGT 3 cut(s) 357, 864, 2946
PshAI GACNNNNGTC 1 cut(s) 152
PshBI ATTAAT 1 cut(s) 21
Psp124BI GAGCTC 3 cut(s) 549, 1527, 2858
Psp5II RGGWCCY 1 cut(s) 713
Psp6I CCWGG 5 cut(s) 224, 619, 1282, 2560, 2849
PspEI GGTNACC 1 cut(s) 2177
PspGI CCWGG 5 cut(s) 224, 619, 1282, 2560, 2849
PspPI GGNCC 8 cut(s) 166, 526, 713, 779, 1511, 1811, 1855, 2240
PspPPI RGGWCCY 1 cut(s) 713
PstI CTGCAG 2 cut(s) 712, 1129
PstNI CAGNNNCTG 1 cut(s) 716
PsuI RGATCY 6 cut(s) 637, 1462, 2150, 2359, 2599, 2770
RsaI GTAC 9 cut(s) 29, 128, 810, 1147, 1360, 2566, 2846, 2912, 2951
RsaNI GTAC 9 cut(s) 28, 127, 809, 1146, 1359, 2565, 2845, 2911, 2950
RseI CAYNNNNRTG 1 cut(s) 2951
SacI GAGCTC 3 cut(s) 549, 1527, 2858
SapI GCTCTTC 3 cut(s) 966, 1158, 3045
Sau96I GGNCC 8 cut(s) 166, 526, 713, 779, 1511, 1811, 1855, 2240
ScaI AGTACT 2 cut(s) 128, 810
SchI GAGTC 4 cut(s) 170, 551, 2078, 2411
ScrFI CCNGG 6 cut(s) 226, 530, 621, 1284, 2562, 2851
SfcI CTRYAG 3 cut(s) 708, 1125, 2478
SfoI GGCGCC 1 cut(s) 55
Sfr274I CTCGAG 2 cut(s) 230, 2892
SfuI TTCGAA 1 cut(s) 452
SinI GGWCC 8 cut(s) 166, 526, 713, 779, 1511, 1811, 1855, 2240
SlaI CTCGAG 2 cut(s) 230, 2892
SmiMI CAYNNNNRTG 1 cut(s) 2951
SmlI CTYRAG 6 cut(s) 230, 1037, 1202, 1892, 2087, 2892
SmoI CTYRAG 6 cut(s) 230, 1037, 1202, 1892, 2087, 2892
SnaBI TACGTA 1 cut(s) 3124
SpeI ACTAGT 1 cut(s) 1132
SspDI GGCGCC 1 cut(s) 53
SspI AATATT 2 cut(s) 448, 2397
SspMI CTAG 8 cut(s) 413, 848, 1133, 1227, 1590, 1995, 2430, 2775
SstI GAGCTC 3 cut(s) 549, 1527, 2858
StyD4I CCNGG 6 cut(s) 224, 528, 619, 1282, 2560, 2849
StyI CCWWGG 4 cut(s) 425, 1246, 1262, 2959
TaaI ACNGT 6 cut(s) 279, 1606, 1694, 1808, 2479, 2630
TaiI ACGT 5 cut(s) 182, 340, 2209, 2955, 3126
TaqI TCGA 6 cut(s) 156, 231, 452, 553, 1874, 2893
TaqII GACCGA 2 cut(s) 539, 767
TatI WGTACW 4 cut(s) 126, 808, 1145, 2844
TauI GCSGC 5 cut(s) 54, 2013, 2174, 2272, 2583
TfiI GAWTC 4 cut(s) 91, 2081, 2234, 2377
TscAI CASTG 3 cut(s) 754, 1545, 2545
TseFI GTSAC 4 cut(s) 145, 2161, 2177, 2434
Tsp45I GTSAC 4 cut(s) 145, 2161, 2177, 2434
TspDTI ATGAA 6 cut(s) 914, 1711, 2130, 2752, 3000, 3038
TspGWI ACGGA 2 cut(s) 2163, 2329
TspRI CASTG 3 cut(s) 754, 1545, 2545
Vha464I CTTAAG 3 cut(s) 1037, 1892, 2087
VneI GTGCAC 1 cut(s) 1884
VpaK11BI GGWCC 8 cut(s) 166, 526, 713, 779, 1511, 1811, 1855, 2240
VspI ATTAAT 1 cut(s) 21
XapI RAATTY 5 cut(s) 768, 830, 1912, 2516, 2695
XceI RCATGY 4 cut(s) 361, 868, 1261, 2950
XcmI CCANNNNNNNNNTGG 1 cut(s) 130
XhoI CTCGAG 2 cut(s) 230, 2892
XmiI GTMKAC 1 cut(s) 1577
XmnI GAANNNNTTC 3 cut(s) 434, 1032, 2590
XspI CTAG 8 cut(s) 413, 848, 1133, 1227, 1590, 1995, 2430, 2775
ZrmI AGTACT 2 cut(s) 128, 810
Zsp2I ATGCAT 1 cut(s) 2614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.