Prupe.1G159700_v2.0.a1

Belongs to the glycosyltransferase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0011248
Physical Location & Seq
Reverse (-)
12702147 .. 12709396
7250 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G159700.3

Sequence Viewer

Length: 3207 bp
ATGGCTGGAAATGAGTGGATCAATGGGTACTTGGAGGCCATACTGGACAGTGGGTCTTCCGCCATTGAAGAGCAGAAGCCGGTGCCGGAGAATCTGAGAGACAGAGGGAATTTCAACCCAACCAAGTACTTTGTGGAGGAGGTGGTGACAGGGGTCGATGAGTCTGACCTCTACCGGACATGGATCAAAGTGGTTGCTACCCGCAACACACGTGAGCGCGGCTCCAGGCTAGAGAACATGTGCTGGCGCATTTGGCATCTTACCCGTAAGAAGAAACAGTTGGAGATCGAGGAACATCAACGGTTAGCAAACCGCAGATGGGAGCGGGAACAAGGACGTAGGGATGCAACTGAAGACATGTCAGAAGATTTGTCAGAAGGAGAGAAGGGGGATGGTTTGGGGGAGATGCTGCCATCTGATACTCCAAGGAAGAAGTTCCAGCGTAACATTTCTAACTTGGAAGTGTGGTCAGATGATAAAAAGGAAAAGAAGCTATATATTGTTCTTATCAGTTTGCATGGTTTGGTCCGGGGAGAAAACATGGAGCTCGGTCGAGACTCTGATACAGGTGGACAGGTCAAATATGTGGTAGAGCTTTCTCGAGCTCTTGCAAGGATGCCTGGGGTTTATAGGGTAGATCTCTTTACTCGCCAAGTGTCATCTCCTGAGGTTGATTGGAGTTATGGTGAACCAGCAGAGATGCTAACTGCAGGTCCTGAAGATGGTGATGGTGATCTAGGAGAAAGTAGTGGAGCATATATTATTAGAATCCCATTTGGTCCACGTGATCAATATCTCAGCAAAGAATTACTCTGGCCATATATTCAAGAATTTGTAGATGGTGCTCTAGCTCACATTCTTAACATGTCCAAAGTTTTGGGTGAACAGATTGGTAAAGGCCAGCCTGTTTGGCCATATGTAATTCATGGCCACTATGCAGATGCAGGGGATAGTGCTGCTCTTCTTTCTGGTGCTTTGAATGTCCCAATGGTTCTAACAGGACACTCACTTGGAAGAAACAAGCTAGAACAGCTTCTTAAGCAGGGACGCCAATCAAAGGAGGACATCAATTCAACATATAAGATTATGAGGAGGATAGAAGCAGAAGAGCTTTCCCTTGATGCTGCAGAAGTTGTTATTACAAGTACAAAACAAGAGATCGACGAGCAATGGGGACTTTATGATGGATTTGATGTCAAGCTTGAGAAAGTGTTGCGTGCTCGTGCTAGGCGAGGAGTAAATTGCCATGGCCGTTACATGCCGAGGATGGTGGTTATACCTCCGGGCATGGACTTCAGTAATGTTGTGGTCCAAGAAGATGCTCCTGAGGTTGATGGGGAACTTACTCAACTTACTGGTGGTACCGATGGGTCTTCCCCAAAAGCACTTCCAACAATATGGTCAGAACTGATGCGGTTCCTTACAAATCCTCACAAGCCAATGATCTTGGCATTGTCAAGACCAGATCCAAAGAAGAACTTAACCACTCTTTTGAAAGCCTTTGGAGAATGCCGTCCTCTGAGAGACCTAGCTAATCTTACGCTGATAATGGGGAATAGGGACTATATAGATGAGATGTCTGCTGGAAATGCTAGTGTGCTTACCACAGTGTTGAAACTGATTGATAAGTATGACCTTTATGGGCAAGTGGCCTATCCAAAGCATCACAAGCAATCAGATGTTCCAGATATATATCGGCTTGCTGCAAAAACAAAGGGAGTTTTCATAAATCCAGCATTAGTGGAGCCCTTTGGGCTAACCTTGATTGAGGCAGCAGCACACGGGCTTCCAATGGTGGCTACAAAAAATGGTGGACCGGTTGACATACATCGGGCGCTAAATAACGGCTTGCTTGTGGACCCTCATGATCAGCAATCAATTGCTGGTGCACTGCTTAAGTTGTTATCGGAGAAGAATTTGTGGGGTGAATGCAGAAAGAACGGTTGGAAGAACATACACCTCTACTCATGGCCTGAACACTGCCGGACTTACTTGACTAGGGTAGCAGCCTGCCGCATGAGGCATCCCCAATGGCAGACTGACACCCCAGAGGATGAAATGGCTGCTGAAGGGTCTCTCAATGACTCCCTTAAGGATGTACAAGATATGTCTCTTAGGCTCTCAGTTGATGGGGATAAATCTTCATTGAATGAATCTCTTGACGTGACTGCAGCTGCTGGTGATCACGAGGTGCAAGATCAAGTGAAACGTGTCCTAAGCAAGATGAAGAAACCAGAGTATGGTCCAAAAGATGAAGGAGGTGGAAATAAGCTGCTGGATAATGTGGCAAGTAAGTATCCCATGTTAAGGAGGAGACGGAAATTGATTGTTGTCGCGCTTGATTGCTATGACAGTTCCGGATCTCCTGAAAAGCAGATGATTCAGGTAGTTCAAGAAATATTTAAGGCTGTCAGATTAGACTCTCAAAGTGCTAGAGTCACAGGGTTTGCTCTATTAACGGCTATGCCAATGTCAGAAACAGTAGAGTTTTTGGCATCGGGGAAGATACAGGCAAATGAGTTTGATGCGTTGGTTTGTAGCAGTGGAAGTGAAGTTTACTACCCTGGTACTTATACAGAAGAAGATGGAAGACTTTTCCCTGATCCAGATTATGCATCACATATTGACTACCGTTGGGGTTGTGAAGGTTTAAAGAAAACCATTTGGAAGCTGTTGAATGCCCCTGATGGTGATCGAAATTCTGCGGCTTCCAGCCACATTCAGGAAGATTTGAAATCAAGCAACGCTCATTGCATCTCATACTTAATAAAGGATCCTAGTAAGGCAAGGAAAGTTGATGATTTGAGGCAGAAACTCAGGATGCGGGGTCTGCGTTGCCATCCAATGTACTCCAGGAGCTCAACAAGAATGCAAATTGTTCCTCTCCTTGCTTCTCGAGCACAGGCACTAAGGTACCTTTTTGTTCGTTGGAGATTGAATGTTGCAAACATGTATGTTTTCCTTGGAGACAGTGGGGACACCGATTATGAAGAAATGATATCCGGGACTCATAAGACCATAATCATGAAAGGGGTGGTGGCAAAGGGTTCAGAAGAGCTGCTAAGAACATCTGGAAGCTATCTAAGAGATGACATTGTTCCTCCAGAGAGCCCCTTGGTCACCTATGTAAGTGGGAAAGCGAAAGCTGATGAGATTGCCAATGCTCTGAAGCAAGTCTCTAAATCTGCTGCTGGAATGTTACTCTAA

Protein Analysis

1069

Amino Acids

119.69

Weight (kDa)

6.16

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011248)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04920
fragaria_vesca FvH4_4g13520 FvH4_4g13520 FvH4_4g13520
malus_domestica MD04G1013500.v1.1
prunus_persica Prupe.1G159700_v2.0.a1 Prupe.1G159700_v2.0.a1 Prupe.1G159700_v2.0.a1
pyrus_communis pycom04g01050
rosa_chinensis RchiOBHm_Chr4g0414151
rosa_laevigata RLG00000008166
rosa_multiflora Rmu_sc0001063.1_g000015
rosa_roxburghii Rroxscaffold_5G00358160
rosa_rugosa Rorug04G0126600
rosa_samantha Rh4AG184700 Rh4BG184100 Rh4CG196400 Rh4DG180000
rosa_wichuraiana Rw0G005040 Rw4G015730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 701
Acc65I GGTACC 2 cut(s) 1361, 2913
AccB1I GGYRCC 3 cut(s) 82, 1361, 2913
AccB7I CCANNNNNTGG 1 cut(s) 2240
AccBSI CCGCTC 1 cut(s) 325
AccII CGCG 2 cut(s) 219, 2336
AccIII TCCGGA 1 cut(s) 2357
AciI CCGC 9 cut(s) 60, 202, 219, 313, 325, 1414, 2014, 2705, 2824
AclWI GGATC 7 cut(s) 26, 191, 1460, 2368, 2597, 2768, 2781
AcoI YGGCCR 4 cut(s) 815, 911, 928, 1249
AcsI RAATTY 4 cut(s) 109, 830, 1915, 2698
AcuI CTGAAG 5 cut(s) 372, 738, 1279, 2088, 3188
AcvI CACGTG 2 cut(s) 212, 785
AcyI GRCGYC 1 cut(s) 1048
AdeI CACNNNGTG 1 cut(s) 2191
AfaI GTAC 8 cut(s) 29, 128, 1147, 1363, 2100, 2569, 2849, 2915
AfiI CCNNNNNNNGG 6 cut(s) 319, 722, 1057, 2240, 2307, 2722
AflII CTTAAG 3 cut(s) 1037, 1895, 2090
AflIII ACRYGT 6 cut(s) 209, 237, 357, 864, 2209, 2949
AgeI ACCGGT 1 cut(s) 1816
AhdI GACNNNNNGTC 1 cut(s) 52
AjiI CACGTC 1 cut(s) 2164
AjnI CCWGG 4 cut(s) 224, 619, 2563, 2852
AjuI GAANNNNNNNTTGG 4 cut(s) 263, 295, 1927, 1959
Alw21I GWGCWC 7 cut(s) 549, 607, 847, 1222, 1891, 2861, 2902
Alw26I GTCTC 8 cut(s) 93, 549, 1518, 2079, 2115, 2308, 2961, 3181
Alw44I GTGCAC 1 cut(s) 1887
AlwI GGATC 7 cut(s) 26, 191, 1460, 2368, 2597, 2768, 2781
AlwNI CAGNNNCTG 2 cut(s) 716, 2177
Ama87I CYCGRG 2 cut(s) 600, 2895
Aor13HI TCCGGA 1 cut(s) 2357
AoxI GGCC 8 cut(s) 36, 815, 898, 911, 928, 1249, 1650, 1970
ApaLI GTGCAC 1 cut(s) 1887
ApoI RAATTY 4 cut(s) 109, 830, 1915, 2698
AsiGI ACCGGT 1 cut(s) 1816
Asp700I GAANNNNTTC 2 cut(s) 434, 1032
Asp718I GGTACC 2 cut(s) 1361, 2913
AspLEI GCGC 4 cut(s) 219, 249, 1837, 2338
AspS9I GGNCC 7 cut(s) 526, 713, 779, 1309, 1814, 1858, 2243
AsuC2I CCSGG 3 cut(s) 530, 1284, 3004
AsuHPI GGTGA 9 cut(s) 157, 698, 737, 743, 893, 1937, 2192, 2702, 3112
AvaI CYCGRG 2 cut(s) 600, 2895
AvaII GGWCC 7 cut(s) 526, 713, 779, 1309, 1814, 1858, 2243
AxyI CCTNAGG 2 cut(s) 666, 1326
BaeGI GKGCMC 1 cut(s) 1891
BaeI ACNNNNGTAYC 2 cut(s) 2897, 2930
BalI TGGCCA 3 cut(s) 817, 913, 930
BamHI GGATCC 1 cut(s) 2773
BanI GGYRCC 3 cut(s) 82, 1361, 2913
BanII GRGCYC 5 cut(s) 549, 607, 1749, 2861, 3113
BauI CACGAG 2 cut(s) 1221, 2186
BbrPI CACGTG 2 cut(s) 212, 785
BbsI GAAGAC 4 cut(s) 48, 360, 1365, 2596
Bbv12I GWGCWC 7 cut(s) 549, 607, 847, 1222, 1891, 2861, 2902
BceAI ACGGC 4 cut(s) 1236, 1497, 1861, 2475
BciT130I CCWGG 4 cut(s) 226, 621, 2565, 2854
BciVI GTATCC 1 cut(s) 2307
BclI TGATCA 3 cut(s) 787, 1867, 2182
BcnI CCSGG 3 cut(s) 530, 1284, 3004
BcoDI GTCTC 8 cut(s) 93, 549, 1518, 2079, 2115, 2308, 2961, 3181
BfmI CTRYAG 3 cut(s) 708, 1125, 2169
BfoI RGCGCY 1 cut(s) 1838
BfrI CTTAAG 3 cut(s) 1037, 1895, 2090
BfuAI ACCTGC 1 cut(s) 701
BfuI GTATCC 1 cut(s) 2307
BglI GCCNNNNNGGC 2 cut(s) 910, 1753
BglII AGATCT 1 cut(s) 637
BmcAI AGTACT 1 cut(s) 128
Bme1390I CCNGG 7 cut(s) 226, 530, 621, 1284, 2565, 2854, 3004
Bme18I GGWCC 7 cut(s) 526, 713, 779, 1309, 1814, 1858, 2243
BmeRI GACNNNNNGTC 1 cut(s) 52
BmeT110I CYCGRG 2 cut(s) 600, 2895
BmgBI CACGTC 1 cut(s) 2164
BmgT120I GGNCC 7 cut(s) 526, 713, 779, 1309, 1814, 1858, 2243
BmiI GGNNCC 8 cut(s) 84, 223, 1363, 1418, 1746, 1860, 2775, 2915
BmrFI CCNGG 7 cut(s) 226, 530, 621, 1284, 2565, 2854, 3004
BoxI GACNNNNGTC 1 cut(s) 152
BpiI GAAGAC 4 cut(s) 48, 360, 1365, 2596
BplI GAGNNNNNCTC 2 cut(s) 206, 238
BpmI CTGGAG 3 cut(s) 208, 2836, 3087
Bpu10I CCTNAGC 1 cut(s) 2216
BpuEI CTTGAG 1 cut(s) 1223
BpuMI CCSGG 3 cut(s) 530, 1284, 3004
BsaAI YACGTR 2 cut(s) 212, 785
BsaBI GATNNNNATC 4 cut(s) 732, 792, 1692, 2691
BsaHI GRCGYC 1 cut(s) 1048
BsaI GGTCTC 2 cut(s) 1518, 2079
BsaJI CCNNGG 8 cut(s) 425, 529, 620, 1246, 1262, 2563, 2962, 3114
BsaWI WCCGGW 3 cut(s) 174, 1816, 2357
BsaXI ACNNNNNCTCC 2 cut(s) 670, 700
Bsc4I CCNNNNNNNGG 6 cut(s) 319, 722, 1057, 2240, 2307, 2722
Bse118I RCCGGY 2 cut(s) 79, 1816
Bse1I ACTGG 2 cut(s) 48, 1360
Bse21I CCTNAGG 2 cut(s) 666, 1326
Bse3DI GCAATG 2 cut(s) 1175, 2749
Bse8I GATNNNNATC 4 cut(s) 732, 792, 1692, 2691
BseAI TCCGGA 1 cut(s) 2357
BseBI CCWGG 4 cut(s) 226, 621, 2565, 2854
BseDI CCNNGG 8 cut(s) 425, 529, 620, 1246, 1262, 2563, 2962, 3114
BseGI GGATG 9 cut(s) 349, 397, 621, 1272, 2023, 2059, 2101, 2826, 2839
BseJI GATNNNNATC 4 cut(s) 732, 792, 1692, 2691
BseLI CCNNNNNNNGG 6 cut(s) 319, 722, 1057, 2240, 2307, 2722
BseMI GCAATG 2 cut(s) 1175, 2749
BseMII CTCAG 7 cut(s) 86, 657, 811, 1317, 1511, 2136, 2830
BseNI ACTGG 2 cut(s) 48, 1360
BseRI GAGGAG 4 cut(s) 152, 1105, 1248, 2326
BseSI GKGCMC 1 cut(s) 1891
Bsh1236I CGCG 2 cut(s) 219, 2336
Bsh1285I CGRYCG 1 cut(s) 553
BshFI GGCC 8 cut(s) 38, 817, 900, 913, 930, 1251, 1652, 1972
BshNI GGYRCC 3 cut(s) 82, 1361, 2913
BshTI ACCGGT 1 cut(s) 1816
BsiEI CGRYCG 1 cut(s) 553
BsiHKAI GWGCWC 7 cut(s) 549, 607, 847, 1222, 1891, 2861, 2902
BsiHKCI CYCGRG 2 cut(s) 600, 2895
BsiSI CCGG 9 cut(s) 80, 86, 175, 529, 1283, 1817, 1984, 2358, 3003
BslFI GGGAC 6 cut(s) 968, 1059, 1188, 1574, 2990, 3019
BslI CCNNNNNNNGG 6 cut(s) 319, 722, 1057, 2240, 2307, 2722
BsmAI GTCTC 8 cut(s) 93, 549, 1518, 2079, 2115, 2308, 2961, 3181
BsmBI CGTCTC 1 cut(s) 2308
BsmFI GGGAC 6 cut(s) 968, 1059, 1188, 1574, 2990, 3019
BsmI GAATGC 4 cut(s) 1514, 1934, 2683, 2874
BsnI GGCC 8 cut(s) 38, 817, 900, 913, 930, 1251, 1652, 1972
Bso31I GGTCTC 2 cut(s) 1518, 2079
BsoBI CYCGRG 2 cut(s) 600, 2895
Bsp1286I GDGCHC 9 cut(s) 549, 607, 847, 1222, 1749, 1891, 2861, 2902, 3113
Bsp13I TCCGGA 1 cut(s) 2357
Bsp1407I TGTACA 1 cut(s) 2098
Bsp19I CCATGG 1 cut(s) 1246
BspACI CCGC 9 cut(s) 60, 202, 219, 313, 325, 1414, 2014, 2705, 2824
BspANI GGCC 8 cut(s) 38, 817, 900, 913, 930, 1251, 1652, 1972
BspCNI CTCAG 7 cut(s) 87, 658, 810, 1318, 1512, 2135, 2829
BspEI TCCGGA 1 cut(s) 2357
BspFNI CGCG 2 cut(s) 219, 2336
BspHI TCATGA 2 cut(s) 1864, 3024
BspLI GGNNCC 8 cut(s) 84, 223, 1363, 1418, 1746, 1860, 2775, 2915
BspMAI CTGCAG 3 cut(s) 712, 1129, 2173
BspMI ACCTGC 1 cut(s) 701
BspPI GGATC 7 cut(s) 26, 191, 1460, 2368, 2597, 2768, 2781
BspQI GCTCTTC 4 cut(s) 63, 966, 1101, 3048
BspT107I GGYRCC 3 cut(s) 82, 1361, 2913
BspTI CTTAAG 3 cut(s) 1037, 1895, 2090
BspTNI GGTCTC 2 cut(s) 1518, 2079
BsrBI CCGCTC 1 cut(s) 325
BsrDI GCAATG 2 cut(s) 1175, 2749
BsrFI RCCGGY 2 cut(s) 79, 1816
BsrGI TGTACA 1 cut(s) 2098
BsrI ACTGG 2 cut(s) 48, 1360
BssAI RCCGGY 2 cut(s) 79, 1816
BssECI CCNNGG 8 cut(s) 425, 529, 620, 1246, 1262, 2563, 2962, 3114
BssNI GRCGYC 1 cut(s) 1048
BssSI CACGAG 2 cut(s) 1221, 2186
BssT1I CCWWGG 4 cut(s) 425, 1246, 2962, 3114
Bst2BI CACGAG 2 cut(s) 1221, 2186
Bst2UI CCWGG 4 cut(s) 226, 621, 2565, 2854
Bst4CI ACNGT 9 cut(s) 50, 279, 303, 1609, 1943, 2354, 2482, 2633, 2972
Bst6I CTCTTC 4 cut(s) 63, 966, 1101, 3048
BstACI GRCGYC 1 cut(s) 1048
BstAFI CTTAAG 3 cut(s) 1037, 1895, 2090
BstAUI TGTACA 1 cut(s) 2098
BstBAI YACGTR 2 cut(s) 212, 785
BstC8I GCNNGC 6 cut(s) 245, 902, 1218, 1701, 1850, 2011
BstDSI CCRYGG 1 cut(s) 1246
BstEII GGTNACC 1 cut(s) 3118
BstF5I GGATG 9 cut(s) 349, 397, 621, 1272, 2023, 2059, 2101, 2826, 2839
BstFNI CGCG 2 cut(s) 219, 2336
BstH2I RGCGCY 1 cut(s) 1838
BstHHI GCGC 4 cut(s) 219, 249, 1837, 2338
BstMAI GTCTC 8 cut(s) 93, 549, 1518, 2079, 2115, 2308, 2961, 3181
BstMCI CGRYCG 1 cut(s) 553
BstMWI GCNNNNNNNGC 9 cut(s) 253, 910, 1030, 1039, 1589, 1669, 1753, 2830, 2897
BstNI CCWGG 4 cut(s) 226, 621, 2565, 2854
BstNSI RCATGY 5 cut(s) 241, 361, 868, 1261, 2953
BstPAI GACNNNNGTC 1 cut(s) 152
BstPI GGTNACC 1 cut(s) 3118
BstSCI CCNGG 7 cut(s) 224, 528, 619, 1282, 2563, 2852, 3002
BstSFI CTRYAG 3 cut(s) 708, 1125, 2169
BstSLI GKGCMC 1 cut(s) 1891
BstUI CGCG 2 cut(s) 219, 2336
BstV2I GAAGAC 4 cut(s) 48, 360, 1365, 2596
BstX2I RGATCY 4 cut(s) 637, 1465, 2360, 2773
BstXI CCANNNNNNTGG 2 cut(s) 877, 1398
BstYI RGATCY 4 cut(s) 637, 1465, 2360, 2773
Bsu36I CCTNAGG 2 cut(s) 666, 1326
BsuI GTATCC 1 cut(s) 2307
BsuRI GGCC 8 cut(s) 38, 817, 900, 913, 930, 1251, 1652, 1972
BtgI CCRYGG 1 cut(s) 1246
BtrI CACGTC 1 cut(s) 2164
BtsCI GGATG 9 cut(s) 349, 397, 621, 1272, 2023, 2059, 2101, 2826, 2839
BtsI GCAGTG 3 cut(s) 1889, 1978, 2548
BtsIMutI CAGTG 6 cut(s) 55, 1614, 1889, 1978, 2548, 2977
BveI ACCTGC 1 cut(s) 701
Cac8I GCNNGC 6 cut(s) 245, 902, 1218, 1701, 1850, 2011
CaiI CAGNNNCTG 2 cut(s) 716, 2177
CciI TCATGA 2 cut(s) 1864, 3024
CfoI GCGC 4 cut(s) 219, 249, 1837, 2338
Cfr10I RCCGGY 2 cut(s) 79, 1816
Cfr13I GGNCC 7 cut(s) 526, 713, 779, 1309, 1814, 1858, 2243
CseI GACGC 1 cut(s) 1056
Csp6I GTAC 8 cut(s) 28, 127, 1146, 1362, 2099, 2568, 2848, 2914
CspAI ACCGGT 1 cut(s) 1816
CviQI GTAC 8 cut(s) 28, 127, 1146, 1362, 2099, 2568, 2848, 2914
DraI TTTAAA 1 cut(s) 2652
DraIII CACNNNGTG 1 cut(s) 2191
DriI GACNNNNNGTC 1 cut(s) 52
EaeI YGGCCR 4 cut(s) 815, 911, 928, 1249
Eam1104I CTCTTC 4 cut(s) 63, 966, 1101, 3048
Eam1105I GACNNNNNGTC 1 cut(s) 52
EarI CTCTTC 4 cut(s) 63, 966, 1101, 3048
EciI GGCGGA 1 cut(s) 49
Ecl136II GAGCTC 3 cut(s) 547, 605, 2859
Eco130I CCWWGG 4 cut(s) 425, 1246, 2962, 3114
Eco24I GRGCYC 5 cut(s) 549, 607, 1749, 2861, 3113
Eco31I GGTCTC 2 cut(s) 1518, 2079
Eco32I GATATC 1 cut(s) 3000
Eco47I GGWCC 7 cut(s) 526, 713, 779, 1309, 1814, 1858, 2243
Eco53kI GAGCTC 3 cut(s) 547, 605, 2859
Eco57I CTGAAG 5 cut(s) 372, 738, 1279, 2088, 3188
Eco72I CACGTG 2 cut(s) 212, 785
Eco81I CCTNAGG 2 cut(s) 666, 1326
Eco88I CYCGRG 2 cut(s) 600, 2895
Eco91I GGTNACC 1 cut(s) 3118
EcoICRI GAGCTC 3 cut(s) 547, 605, 2859
EcoO109I RGGNCCY 1 cut(s) 713
EcoO65I GGTNACC 1 cut(s) 3118
EcoRII CCWGG 4 cut(s) 224, 619, 2563, 2852
EcoRV GATATC 1 cut(s) 3000
EcoT14I CCWWGG 4 cut(s) 425, 1246, 2962, 3114
EcoT22I ATGCAT 1 cut(s) 2617
EcoT38I GRGCYC 5 cut(s) 549, 607, 1749, 2861, 3113
ErhI CCWWGG 4 cut(s) 425, 1246, 2962, 3114
Esp3I CGTCTC 1 cut(s) 2308
FalI AAGNNNNNCTT 2 cut(s) 1463, 1495
FaqI GGGAC 6 cut(s) 968, 1059, 1188, 1574, 2990, 3019
FauI CCCGC 3 cut(s) 209, 318, 2817
FauNDI CATATG 1 cut(s) 916
FbaI TGATCA 3 cut(s) 787, 1867, 2182
FokI GGATG 9 cut(s) 356, 404, 628, 1279, 2010, 2066, 2108, 2826, 2833
FriOI GRGCYC 5 cut(s) 549, 607, 1749, 2861, 3113
GlaI GCGC 4 cut(s) 218, 248, 1836, 2337
GsuI CTGGAG 3 cut(s) 208, 2836, 3087
HaeII RGCGCY 1 cut(s) 1838
HaeIII GGCC 8 cut(s) 38, 817, 900, 913, 930, 1251, 1652, 1972
HapII CCGG 9 cut(s) 80, 86, 175, 529, 1283, 1817, 1984, 2358, 3003
HgaI GACGC 1 cut(s) 1056
HhaI GCGC 4 cut(s) 219, 249, 1837, 2338
Hin1I GRCGYC 1 cut(s) 1048
Hin6I GCGC 4 cut(s) 217, 247, 1835, 2336
HinP1I GCGC 4 cut(s) 217, 247, 1835, 2336
HincII GTYRAC 1 cut(s) 1822
HindII GTYRAC 1 cut(s) 1822
HindIII AAGCTT 1 cut(s) 1199
HpaII CCGG 9 cut(s) 80, 86, 175, 529, 1283, 1817, 1984, 2358, 3003
HphI GGTGA 9 cut(s) 157, 698, 737, 743, 893, 1937, 2192, 2702, 3112
Hpy166II GTNNAC 9 cut(s) 572, 689, 782, 884, 1814, 1822, 1858, 1889, 2557
Hpy8I GTNNAC 9 cut(s) 572, 689, 782, 884, 1814, 1822, 1858, 1889, 2557
Hpy99I CGWCG 1 cut(s) 1166
HpyAV CCTTC 5 cut(s) 371, 379, 2063, 2249, 2639
HpyCH4III ACNGT 9 cut(s) 50, 279, 303, 1609, 1943, 2354, 2482, 2633, 2972
HpyCH4IV ACGT 5 cut(s) 211, 337, 784, 2163, 2209
HpyF10VI GCNNNNNNNGC 9 cut(s) 253, 910, 1030, 1039, 1589, 1669, 1753, 2830, 2897
HpySE526I ACGT 5 cut(s) 211, 337, 784, 2163, 2209
Hsp92I GRCGYC 1 cut(s) 1048
HspAI GCGC 4 cut(s) 217, 247, 1835, 2336
Kpn2I TCCGGA 1 cut(s) 2357
KpnI GGTACC 2 cut(s) 1365, 2917
Ksp22I TGATCA 3 cut(s) 787, 1867, 2182
LguI GCTCTTC 4 cut(s) 63, 966, 1101, 3048
LmnI GCTCC 7 cut(s) 227, 322, 544, 752, 1327, 1744, 2856
MaeII ACGT 5 cut(s) 211, 337, 784, 2163, 2209
MaeIII GTNAC 7 cut(s) 145, 443, 1253, 2164, 2437, 3118, 3198
MbiI CCGCTC 1 cut(s) 325
MfeI CAATTG 1 cut(s) 1878
MflI RGATCY 4 cut(s) 637, 1465, 2360, 2773
MhlI GDGCHC 9 cut(s) 549, 607, 847, 1222, 1749, 1891, 2861, 2902, 3113
MlsI TGGCCA 3 cut(s) 817, 913, 930
MluNI TGGCCA 3 cut(s) 817, 913, 930
MlyI GAGTC 6 cut(s) 170, 551, 2078, 2414, 2445, 3001
MmeI TCCRAC 4 cut(s) 261, 1415, 1925, 2909
Mox20I TGGCCA 3 cut(s) 817, 913, 930
Mph1103I ATGCAT 1 cut(s) 2617
MroI TCCGGA 1 cut(s) 2357
MroXI GAANNNNTTC 2 cut(s) 434, 1032
MscI TGGCCA 3 cut(s) 817, 913, 930
MslI CAYNNNNRTG 1 cut(s) 3023
Msp20I TGGCCA 3 cut(s) 817, 913, 930
MspA1I CMGCKG 1 cut(s) 2174
MspCI CTTAAG 3 cut(s) 1037, 1895, 2090
MspI CCGG 9 cut(s) 80, 86, 175, 529, 1283, 1817, 1984, 2358, 3003
MspR9I CCNGG 7 cut(s) 226, 530, 621, 1284, 2565, 2854, 3004
MunI CAATTG 1 cut(s) 1878
Mva1269I GAATGC 4 cut(s) 1514, 1934, 2683, 2874
MvaI CCWGG 4 cut(s) 226, 621, 2565, 2854
MvnI CGCG 2 cut(s) 219, 2336
MwoI GCNNNNNNNGC 9 cut(s) 253, 910, 1030, 1039, 1589, 1669, 1753, 2830, 2897
NciI CCSGG 3 cut(s) 530, 1284, 3004
NcoI CCATGG 1 cut(s) 1246
NdeI CATATG 1 cut(s) 916
NlaIV GGNNCC 8 cut(s) 84, 223, 1363, 1418, 1746, 1860, 2775, 2915
NmeAIII GCCGAG 1 cut(s) 1287
NmuCI GTSAC 4 cut(s) 145, 2164, 2437, 3118
NsiI ATGCAT 1 cut(s) 2617
NspI RCATGY 5 cut(s) 241, 361, 868, 1261, 2953
PaeR7I CTCGAG 2 cut(s) 600, 2895
PagI TCATGA 2 cut(s) 1864, 3024
PciI ACATGT 4 cut(s) 237, 357, 864, 2949
PciSI GCTCTTC 4 cut(s) 63, 966, 1101, 3048
PcsI WCGNNNNNNNCGW 1 cut(s) 1228
PctI GAATGC 4 cut(s) 1514, 1934, 2683, 2874
PdmI GAANNNNTTC 2 cut(s) 434, 1032
PfeI GAWTC 4 cut(s) 91, 768, 2153, 2380
PflMI CCANNNNNTGG 1 cut(s) 2240
PfoI TCCNGGA 2 cut(s) 2852, 3002
PinAI ACCGGT 1 cut(s) 1816
PleI GAGTC 6 cut(s) 169, 551, 2078, 2414, 2444, 3001
PmaCI CACGTG 2 cut(s) 212, 785
PmlI CACGTG 2 cut(s) 212, 785
PpsI GAGTC 6 cut(s) 169, 551, 2078, 2414, 2444, 3001
Ppu21I YACGTR 2 cut(s) 212, 785
PpuMI RGGWCCY 1 cut(s) 713
PscI ACATGT 4 cut(s) 237, 357, 864, 2949
PshAI GACNNNNGTC 1 cut(s) 152
Psp124BI GAGCTC 3 cut(s) 549, 607, 2861
Psp5II RGGWCCY 1 cut(s) 713
Psp6I CCWGG 4 cut(s) 224, 619, 2563, 2852
PspCI CACGTG 2 cut(s) 212, 785
PspEI GGTNACC 1 cut(s) 3118
PspGI CCWGG 4 cut(s) 224, 619, 2563, 2852
PspN4I GGNNCC 8 cut(s) 84, 223, 1363, 1418, 1746, 1860, 2775, 2915
PspPI GGNCC 7 cut(s) 526, 713, 779, 1309, 1814, 1858, 2243
PspPPI RGGWCCY 1 cut(s) 713
PstI CTGCAG 3 cut(s) 712, 1129, 2173
PstNI CAGNNNCTG 2 cut(s) 716, 2177
PsuI RGATCY 4 cut(s) 637, 1465, 2360, 2773
PvuII CAGCTG 1 cut(s) 2174
RsaI GTAC 8 cut(s) 29, 128, 1147, 1363, 2100, 2569, 2849, 2915
RsaNI GTAC 8 cut(s) 28, 127, 1146, 1362, 2099, 2568, 2848, 2914
RseI CAYNNNNRTG 1 cut(s) 3023
SacI GAGCTC 3 cut(s) 549, 607, 2861
SapI GCTCTTC 4 cut(s) 63, 966, 1101, 3048
Sau96I GGNCC 7 cut(s) 526, 713, 779, 1309, 1814, 1858, 2243
ScaI AGTACT 1 cut(s) 128
SchI GAGTC 6 cut(s) 170, 551, 2078, 2414, 2445, 3001
ScrFI CCNGG 7 cut(s) 226, 530, 621, 1284, 2565, 2854, 3004
SduI GDGCHC 9 cut(s) 549, 607, 847, 1222, 1749, 1891, 2861, 2902, 3113
SfcI CTRYAG 3 cut(s) 708, 1125, 2169
Sfr274I CTCGAG 2 cut(s) 600, 2895
SinI GGWCC 7 cut(s) 526, 713, 779, 1309, 1814, 1858, 2243
SlaI CTCGAG 2 cut(s) 600, 2895
SmiMI CAYNNNNRTG 1 cut(s) 3023
SmlI CTYRAG 6 cut(s) 600, 1037, 1202, 1895, 2090, 2895
SmoI CTYRAG 6 cut(s) 600, 1037, 1202, 1895, 2090, 2895
SsiI CCGC 9 cut(s) 60, 202, 219, 313, 325, 1414, 2014, 2705, 2824
SspI AATATT 1 cut(s) 2400
SstI GAGCTC 3 cut(s) 549, 607, 2861
StyD4I CCNGG 7 cut(s) 224, 528, 619, 1282, 2563, 2852, 3002
StyI CCWWGG 4 cut(s) 425, 1246, 2962, 3114
TaaI ACNGT 9 cut(s) 50, 279, 303, 1609, 1943, 2354, 2482, 2633, 2972
TaiI ACGT 5 cut(s) 214, 340, 787, 2166, 2212
TaqI TCGA 7 cut(s) 156, 288, 553, 601, 1161, 2695, 2896
TaqII GACCGA 1 cut(s) 539
TatI WGTACW 4 cut(s) 126, 1145, 2098, 2847
TauI GCSGC 3 cut(s) 222, 2016, 2708
TfiI GAWTC 4 cut(s) 91, 768, 2153, 2380
TscAI CASTG 6 cut(s) 55, 1614, 1896, 1985, 2548, 2977
TseFI GTSAC 4 cut(s) 145, 2164, 2437, 3118
Tsp45I GTSAC 4 cut(s) 145, 2164, 2437, 3118
TspDTI ATGAA 9 cut(s) 914, 1714, 2070, 2133, 2166, 2240, 2268, 3003, 3041
TspGWI ACGGA 1 cut(s) 2332
TspRI CASTG 6 cut(s) 55, 1614, 1896, 1985, 2548, 2977
Van91I CCANNNNNTGG 1 cut(s) 2240
Vha464I CTTAAG 3 cut(s) 1037, 1895, 2090
VneI GTGCAC 1 cut(s) 1887
VpaK11BI GGWCC 7 cut(s) 526, 713, 779, 1309, 1814, 1858, 2243
XapI RAATTY 4 cut(s) 109, 830, 1915, 2698
XceI RCATGY 5 cut(s) 241, 361, 868, 1261, 2953
XcmI CCANNNNNNNNNTGG 1 cut(s) 130
XhoI CTCGAG 2 cut(s) 600, 2895
XmnI GAANNNNTTC 2 cut(s) 434, 1032
ZrmI AGTACT 1 cut(s) 128
Zsp2I ATGCAT 1 cut(s) 2617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.