FvH4_4g13520

Belongs to the glycosyltransferase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0007500
Physical Location & Seq
Forward (+)
17116630 .. 17122858
6229 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g13520.t1

Sequence Viewer

Length: 3201 bp
ATGGCGGGAAATGAGTGGATCAATGGCTACCTGGAGGCGATACTCGACACCGGGGCGACGGCGATAGAGGAGCAGAAGCCGACGCCGGTGAATCTGAGTGAGGGAGGCCATTTTAATCCGACTAAGTATTTCGTCGAGGAGGTAGTTACTGGGGTTGATGAGTCTGATTTGTACCGGACGTGGATCAAAGTGGTTGCTACCCGCAACACACGAGAGCGCAGCTCCAGGCTCGAGAATATGTGCTGGCGCATTTGGCATCTTACTCGTAAGAAGAAGCAGTTGGAGTGGGAGGAACTTCAGCGGTCGGCGAACCGCAGATGGGAGAGGGAGCAAGGAAGGAGGGATGCAACGGAAGACATGTCGGAGGACTTGTCCGAAGGAGAGAAGGGTGATATATTGGGGGAGATGCTGCAGTGTGAGACTCCAAGGAGAAAGTTCCAGCGGATGGTTTCTAATTTGGAAGTATGGTCAGATGATAAAAAGGAAAAGAAACTATATGTCGTTCTGATCAGTTTGCATGGTTTAGTCCGGGGAGAAAACATGGAGCTTGGTCGAGACTCAGATACAGGTGGACAGGTCAAATATGTTGTAGAGCTTGCCCGAGCACTTGCAAGGATGCCTGGGGTTTACAGGGTAGATCTCTTTACTCGCCAAGTGTCATCTCCTGAAGTTGATTGGAGCTATGGTGAACCAACCGAGATGATAACTGCAGGTCCTGAAGATGGTGATGGTGATCTAGGAGAAAGCAGCGGAGCATATATCATTAGAATTCCTTTTGGTCCTCGTGACCAATACCTTAGCAAAGAAGTACTCTGGCCCCATATTCAAGAATTTGTAGATGGAGCTCTAGCTCACATTCTTAATATGTCCAAAGTTTTGGGTGAACAAATTGGCAAAGGCCAGCCTGTCTGGCCATATGTAATTCATGGCCACTATGCAGATGCAGGGGACAGTGCTGCTCTTCTTTCAGGCGCTTTGAATGTCCCAATGGTTCTAACAGGACACTCACTTGGAAGAAACAAACTCGAACAGCTTATAAAGCAGGGACGCCTATCAAAGGAAGACATCAATTCCACATATAAGATTATGAGGAGGATAGAGGCAGAAGAGCTTTCCCTTGATGCTGCAGAACTTGTTATTACAAGTACAAAGCAAGAGATCGAAGAACAATGGGGACTTTATGATGGGTTTGATGTCAAGCTTGAGAAAGTTTTGCGTGCCCGGGCTAGAAGAGGAGTAAATTGCCATGGTCGTTTCATGCCAAGGATGGTGGTTATACCCCCTGGCATGGACTTCAGTAATGTTATGGTCCAAGAAGATGATGCTGACGGGGAACTTAGCCAGCTGATAGGTGGTAGTGATGGGCCTTCTTCCCCGAAAGCAATACCAACAATCTGGTCAGAAGTGATGAGATTCCTTACTAATCCTCACAAGCCAATGATCTTGGCTTTGTCACGGCCAGATCCAAAGAAGAACATTACCACTCTTTTGAAAGCATTTGGAGAATGCCGTCCTCTAAGAGAACTTGCTAATCTTACACTGATAATGGGGAATAGGGACTATATAGACGAAATGTCTACTGGAAATGCTAGTGTGCTTACTACGGTGTTGAAAATGATCGATAAGTATGATCTCTATGGGCAAGTTGCCTATCCAAAGCATCACAAGCAATCAGATGTTCCGGATATTTATCGGCTTGCAGCAAAAACAAAGGGAGTTTTTATAAATCCAGCACTAGTGGAGCCTTTTGGTCTAACCTTGATTGAGGCAGCAGCACATGGACTTCCTATGGTGGCTACGAAAAATGGTGGACCGGTTGACATACATCGGGCACTTAATAATGGTTTGCTTGTCGACCCTCATGATCAGCAATCAATTGCTAATGCATTGCTGAAGTTGTTATCGGAAAAGAATTTATGGGTTGACTGCAGAAAGAATGGATGGAAAAACATACACCTTTTCTCATGGCCTGAACACTGCCGAACCTACTTGACCAGGGTAGCAGCCTGCCGCATGAGATATCCACAGTGGCAGACTGACACTCCGGAGGATGAAATGGCAGCTGAGGAGTCCTTCAATGATTCTCTCAGGGACGTGCAAGATATGTCTCTTAGGCTCTCAGTTGATGGGGACAAATCCTCACTGAATGAATCTCTAGATGTGACTGCAACTTCTGGCGACCATGAGGTGCAAGATCAAGTGAAGCGTGTCCTAAGCAAGATGAAGAAGTCAGATTCTGGTCCAAAAGACCACGAAGACGGAAATAAGCTGCCGGATAATGTATCCAGCAAGTATCCTTTGTTAAGACGTAGAAGAAAATTGATTGTCATAGCACTTGATTGCTATGACCAATCTGGAGCTCCTGATAAGAAAATTATTCAAGTTGTGCAAGAAATATTTAAGGCTGTAAGACTAGATTCTCAAAGCGCAAGATTTACAGGGTTTGCTCTGCTGACGGCTATGCCAGCTTCGGAGACAGTAGAGTTTTTGGCATCAGGGAAGATTCAGGCAAATGAGTTTGATGCTTTGGTTTGTAGCAGTGGAAGCGAGGTTTACTACCCTGGTACTTATACAGAAGAAGATGGAAGGCTTTTTCCAGATCCAGATTATTCGTCACACATTGACTACAGATGGGGTTGTGAAGGATTAAAGAAAACCATTTGGAAGCTCTTAAATGCCCCTGATGGTGAACGAAATTCAGGATCTTCCAACCAAATTGAAGAAGATTTGAAATCAAGCAATTCCCATTGCATTACATACTTAATCAAGGATCCTAGCAAGGCCAGGAAAGTTGATGATTTAAGGCAGAAACTCAGGATGCGCGGACTACGCTGCCATCCAATGTACTGCAGGAGCTCAACACGAATGCAAATTGTTCCGCTCCTTGCTTCTCGAGCACAGGCTCTCAGGTATCTTTTTGTACGCTGGAGATTGAATGTCGCAAACATGTACGTGTTCCTTGGAGAAAGTGGGGACACCGATTATGAAGAAATGATAGCAGGGACTCACAAGACCATAATCATGAAAGGGGTGGTGGGAAAGGGTTCTGAAGAGCTGCTAAGAACATCAGGAAGCTACGTGAGAGACGACATTGTTCCTCCTCAGAGCCCATTGGTCGCCATTGTAAATGGGCAAGCTCCAACAGCTGATGAGATTGCGACTGCTTTGAAGCAAGTCAGCAAATCTGCTGCTGGAATGTGA

Protein Analysis

1067

Amino Acids

119.79

Weight (kDa)

5.95

Isoelectric Point (pI)

46.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GT-B_Sucrose_synth PF00862 166 - 433 2.8e-12 Sucrose synthase first GT-B domain
Glyco_trans_4_4 PF13579 190 - 388 1.2e-06 Glycosyl transferase 4-like domain
Glycos_transf_1 PF00534 475 - 647 8.9e-24 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 479 - 635 1.6e-15 Glycosyl transferases group 1
S6PP PF05116 840 - 1007 2.6e-12 Sucrose-6F-phosphate phosphohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1037, 1724
Acc36I ACCTGC 1 cut(s) 701
AccB7I CCANNNNNTGG 1 cut(s) 445
AccBSI CCGCTC 1 cut(s) 2881
AccI GTMKAC 2 cut(s) 1577, 1854
AccII CGCG 1 cut(s) 2823
AccIII TCCGGA 2 cut(s) 1681, 2044
AciI CCGC 9 cut(s) 5, 202, 301, 313, 442, 750, 2011, 2823, 2879
AclWI GGATC 7 cut(s) 26, 191, 1457, 2594, 2710, 2765, 2778
AcoI YGGCCR 3 cut(s) 911, 928, 1457
AcsI RAATTY 4 cut(s) 768, 830, 1912, 2695
AcuI CTGAAG 6 cut(s) 281, 687, 738, 1279, 1913, 3069
AcyI GRCGYC 2 cut(s) 83, 1048
AfaI GTAC 7 cut(s) 173, 810, 1147, 2566, 2846, 2922, 2951
AfiI CCNNNNNNNGG 5 cut(s) 319, 445, 722, 1057, 1288
AflIII ACRYGT 3 cut(s) 357, 2946, 2952
AgeI ACCGGT 1 cut(s) 1813
AhdI GACNNNNNGTC 1 cut(s) 1573
AhlI ACTAGT 1 cut(s) 1735
AjiI CACGTC 2 cut(s) 180, 2095
AjnI CCWGG 7 cut(s) 30, 224, 619, 1282, 1994, 2560, 2783
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
Alw21I GWGCWC 5 cut(s) 607, 847, 2362, 2858, 2899
Alw26I GTCTC 5 cut(s) 413, 549, 2112, 2468, 3078
AlwI GGATC 7 cut(s) 26, 191, 1457, 2594, 2710, 2765, 2778
AlwNI CAGNNNCTG 2 cut(s) 716, 2237
Ama87I CYCGRG 4 cut(s) 230, 600, 1221, 2892
Aor13HI TCCGGA 2 cut(s) 1681, 2044
AoxI GGCC 9 cut(s) 106, 815, 898, 911, 928, 1364, 1457, 1967, 2781
ApoI RAATTY 4 cut(s) 768, 830, 1912, 2695
AsiGI ACCGGT 1 cut(s) 1813
Asp700I GAANNNNTTC 1 cut(s) 3043
AspLEI GCGC 5 cut(s) 219, 249, 974, 2429, 2823
AspS9I GGNCC 7 cut(s) 713, 779, 816, 1309, 1364, 1811, 2240
AsuC2I CCSGG 4 cut(s) 52, 530, 1222, 1223
AsuHPI GGTGA 7 cut(s) 100, 401, 698, 737, 743, 893, 2699
AvaI CYCGRG 4 cut(s) 230, 600, 1221, 2892
AvaII GGWCC 5 cut(s) 713, 779, 1309, 1811, 2240
BaeGI GKGCMC 2 cut(s) 1222, 1834
BalI TGGCCA 2 cut(s) 913, 930
BamHI GGATCC 1 cut(s) 2770
BanII GRGCYC 4 cut(s) 847, 2362, 2858, 3110
BauI CACGAG 2 cut(s) 210, 783
BbsI GAAGAC 3 cut(s) 360, 1068, 2262
Bbv12I GWGCWC 5 cut(s) 607, 847, 2362, 2858, 2899
BbvCI CCTCAGC 1 cut(s) 2064
BceAI ACGGC 4 cut(s) 75, 1472, 1494, 2472
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BciT130I CCWGG 7 cut(s) 32, 226, 621, 1284, 1996, 2562, 2785
BciVI GTATCC 2 cut(s) 2293, 2304
BclI TGATCA 2 cut(s) 507, 1864
BcnI CCSGG 4 cut(s) 52, 530, 1222, 1223
BcoDI GTCTC 5 cut(s) 413, 549, 2112, 2468, 3078
BcuI ACTAGT 1 cut(s) 1735
BfaI CTAG 8 cut(s) 737, 848, 1227, 1590, 1736, 2156, 2414, 2775
BfmI CTRYAG 6 cut(s) 410, 708, 1125, 1927, 2626, 2848
BfoI RGCGCY 1 cut(s) 975
BfuAI ACCTGC 1 cut(s) 701
BfuI GTATCC 2 cut(s) 2293, 2304
BglI GCCNNNNNGGC 1 cut(s) 910
BglII AGATCT 1 cut(s) 637
BmcAI AGTACT 1 cut(s) 810
Bme18I GGWCC 5 cut(s) 713, 779, 1309, 1811, 2240
BmeRI GACNNNNNGTC 1 cut(s) 1573
BmeT110I CYCGRG 4 cut(s) 230, 600, 1221, 2892
BmgBI CACGTC 2 cut(s) 180, 2095
BmgT120I GGNCC 7 cut(s) 713, 779, 816, 1309, 1364, 1811, 2240
BmiI GGNNCC 3 cut(s) 818, 1743, 2772
BmrI ACTGGG 1 cut(s) 159
BmuI ACTGGG 1 cut(s) 159
BpiI GAAGAC 3 cut(s) 360, 1068, 2262
BplI GAGNNNNNCTC 2 cut(s) 206, 238
BpmI CTGGAG 4 cut(s) 53, 208, 2376, 2947
Bpu10I CCTNAGC 3 cut(s) 797, 2064, 2213
BpuEI CTTGAG 1 cut(s) 1223
BpuMI CCSGG 4 cut(s) 52, 530, 1222, 1223
Bsa29I ATCGAT 1 cut(s) 1620
BsaAI YACGTR 2 cut(s) 2953, 3079
BsaBI GATNNNNATC 2 cut(s) 732, 1689
BsaHI GRCGYC 2 cut(s) 83, 1048
BsaWI WCCGGW 4 cut(s) 174, 1681, 1813, 2044
BsaXI ACNNNNNCTCC 6 cut(s) 356, 386, 670, 700, 2026, 2056
Bsc4I CCNNNNNNNGG 5 cut(s) 319, 445, 722, 1057, 1288
Bse118I RCCGGY 2 cut(s) 85, 1813
Bse1I ACTGG 2 cut(s) 154, 1585
Bse3DI GCAATG 2 cut(s) 1886, 2746
Bse8I GATNNNNATC 2 cut(s) 732, 1689
BseAI TCCGGA 2 cut(s) 1681, 2044
BseBI CCWGG 7 cut(s) 32, 226, 621, 1284, 1996, 2562, 2785
BseCI ATCGAT 1 cut(s) 1620
BseGI GGATG 8 cut(s) 349, 450, 621, 1272, 1946, 2056, 2823, 2836
BseJI GATNNNNATC 2 cut(s) 732, 1689
BseLI CCNNNNNNNGG 5 cut(s) 319, 445, 722, 1057, 1288
BseMI GCAATG 2 cut(s) 1886, 2746
BseMII CTCAG 8 cut(s) 86, 573, 2055, 2101, 2133, 2827, 2920, 3116
BseNI ACTGG 2 cut(s) 154, 1585
BseRI GAGGAG 6 cut(s) 83, 152, 1105, 1248, 2081, 3090
BseSI GKGCMC 2 cut(s) 1222, 1834
Bsh1236I CGCG 1 cut(s) 2823
Bsh1285I CGRYCG 1 cut(s) 305
BshFI GGCC 9 cut(s) 108, 817, 900, 913, 930, 1366, 1459, 1969, 2783
BshTI ACCGGT 1 cut(s) 1813
BshVI ATCGAT 1 cut(s) 1620
BsiEI CGRYCG 1 cut(s) 305
BsiHKAI GWGCWC 5 cut(s) 607, 847, 2362, 2858, 2899
BsiHKCI CYCGRG 4 cut(s) 230, 600, 1221, 2892
BsiSI CCGG 9 cut(s) 51, 86, 175, 529, 1222, 1682, 1814, 2045, 2273
BslFI GGGAC 9 cut(s) 962, 968, 1059, 1188, 1571, 2105, 2144, 2987, 3016
BslI CCNNNNNNNGG 5 cut(s) 319, 445, 722, 1057, 1288
BsmAI GTCTC 5 cut(s) 413, 549, 2112, 2468, 3078
BsmBI CGTCTC 1 cut(s) 3078
BsmFI GGGAC 9 cut(s) 962, 968, 1059, 1188, 1571, 2105, 2144, 2987, 3016
BsmI GAATGC 2 cut(s) 1511, 2871
BsnI GGCC 9 cut(s) 108, 817, 900, 913, 930, 1366, 1459, 1969, 2783
BsoBI CYCGRG 4 cut(s) 230, 600, 1221, 2892
Bsp1286I GDGCHC 8 cut(s) 607, 847, 1222, 1834, 2362, 2858, 2899, 3110
Bsp13I TCCGGA 2 cut(s) 1681, 2044
Bsp19I CCATGG 1 cut(s) 1246
BspACI CCGC 9 cut(s) 5, 202, 301, 313, 442, 750, 2011, 2823, 2879
BspANI GGCC 9 cut(s) 108, 817, 900, 913, 930, 1366, 1459, 1969, 2783
BspCNI CTCAG 8 cut(s) 87, 572, 2056, 2100, 2132, 2826, 2919, 3115
BspDI ATCGAT 1 cut(s) 1620
BspEI TCCGGA 2 cut(s) 1681, 2044
BspFNI CGCG 1 cut(s) 2823
BspHI TCATGA 2 cut(s) 1861, 3021
BspLI GGNNCC 3 cut(s) 818, 1743, 2772
BspMAI CTGCAG 5 cut(s) 414, 712, 1129, 1931, 2852
BspMI ACCTGC 1 cut(s) 701
BspPI GGATC 7 cut(s) 26, 191, 1457, 2594, 2710, 2765, 2778
BspQI GCTCTTC 3 cut(s) 966, 1101, 3045
BsrBI CCGCTC 1 cut(s) 2881
BsrDI GCAATG 2 cut(s) 1886, 2746
BsrFI RCCGGY 2 cut(s) 85, 1813
BsrI ACTGG 2 cut(s) 154, 1585
BssAI RCCGGY 2 cut(s) 85, 1813
BssNI GRCGYC 2 cut(s) 83, 1048
BssSI CACGAG 2 cut(s) 210, 783
BssT1I CCWWGG 4 cut(s) 425, 1246, 1262, 2959
Bst2BI CACGAG 2 cut(s) 210, 783
Bst2UI CCWGG 7 cut(s) 32, 226, 621, 1284, 1996, 2562, 2785
Bst4CI ACNGT 4 cut(s) 953, 1606, 2028, 2479
Bst6I CTCTTC 4 cut(s) 966, 1101, 1225, 3045
BstACI GRCGYC 2 cut(s) 83, 1048
BstBAI YACGTR 2 cut(s) 2953, 3079
BstC8I GCNNGC 9 cut(s) 245, 597, 902, 1218, 1343, 1698, 2008, 2466, 3135
BstDSI CCRYGG 1 cut(s) 1246
BstENI CCTNNNNNAGG 1 cut(s) 1055
BstF5I GGATG 8 cut(s) 349, 450, 621, 1272, 1946, 2056, 2823, 2836
BstFNI CGCG 1 cut(s) 2823
BstH2I RGCGCY 1 cut(s) 975
BstHHI GCGC 5 cut(s) 219, 249, 974, 2429, 2823
BstMAI GTCTC 5 cut(s) 413, 549, 2112, 2468, 3078
BstMCI CGRYCG 1 cut(s) 305
BstMWI GCNNNNNNNGC 9 cut(s) 253, 910, 1039, 1666, 2465, 2544, 2829, 2894, 3143
BstNI CCWGG 7 cut(s) 32, 226, 621, 1284, 1996, 2562, 2785
BstNSI RCATGY 2 cut(s) 361, 2950
BstSFI CTRYAG 6 cut(s) 410, 708, 1125, 1927, 2626, 2848
BstSLI GKGCMC 2 cut(s) 1222, 1834
BstUI CGCG 1 cut(s) 2823
BstV2I GAAGAC 3 cut(s) 360, 1068, 2262
BstX2I RGATCY 5 cut(s) 637, 1462, 2599, 2702, 2770
BstXI CCANNNNNNTGG 2 cut(s) 877, 1395
BstYI RGATCY 5 cut(s) 637, 1462, 2599, 2702, 2770
Bsu15I ATCGAT 1 cut(s) 1620
BsuI GTATCC 2 cut(s) 2293, 2304
BsuRI GGCC 9 cut(s) 108, 817, 900, 913, 930, 1366, 1459, 1969, 2783
BsuTUI ATCGAT 1 cut(s) 1620
BtgI CCRYGG 1 cut(s) 1246
BtrI CACGTC 2 cut(s) 180, 2095
BtsCI GGATG 8 cut(s) 349, 450, 621, 1272, 1946, 2056, 2823, 2836
BtsI GCAGTG 3 cut(s) 419, 1975, 2545
BtsIMutI CAGTG 7 cut(s) 419, 958, 1538, 1975, 2033, 2141, 2545
BveI ACCTGC 1 cut(s) 701
Cac8I GCNNGC 9 cut(s) 245, 597, 902, 1218, 1343, 1698, 2008, 2466, 3135
CaiI CAGNNNCTG 2 cut(s) 716, 2237
CciI TCATGA 2 cut(s) 1861, 3021
CfoI GCGC 5 cut(s) 219, 249, 974, 2429, 2823
Cfr10I RCCGGY 2 cut(s) 85, 1813
Cfr13I GGNCC 7 cut(s) 713, 779, 816, 1309, 1364, 1811, 2240
Cfr9I CCCGGG 1 cut(s) 1221
ClaI ATCGAT 1 cut(s) 1620
CseI GACGC 2 cut(s) 91, 1056
Csp6I GTAC 7 cut(s) 172, 809, 1146, 2565, 2845, 2921, 2950
CspAI ACCGGT 1 cut(s) 1813
CspCI CAANNNNNGTGG 2 cut(s) 1251, 1286
CviQI GTAC 7 cut(s) 172, 809, 1146, 2565, 2845, 2921, 2950
DriI GACNNNNNGTC 1 cut(s) 1573
EaeI YGGCCR 3 cut(s) 911, 928, 1457
Eam1104I CTCTTC 4 cut(s) 966, 1101, 1225, 3045
Eam1105I GACNNNNNGTC 1 cut(s) 1573
EarI CTCTTC 4 cut(s) 966, 1101, 1225, 3045
Ecl136II GAGCTC 3 cut(s) 845, 2360, 2856
Eco130I CCWWGG 4 cut(s) 425, 1246, 1262, 2959
Eco24I GRGCYC 4 cut(s) 847, 2362, 2858, 3110
Eco32I GATATC 1 cut(s) 2021
Eco47I GGWCC 5 cut(s) 713, 779, 1309, 1811, 2240
Eco53kI GAGCTC 3 cut(s) 845, 2360, 2856
Eco57I CTGAAG 6 cut(s) 281, 687, 738, 1279, 1913, 3069
Eco88I CYCGRG 4 cut(s) 230, 600, 1221, 2892
EcoICRI GAGCTC 3 cut(s) 845, 2360, 2856
EcoNI CCTNNNNNAGG 1 cut(s) 1055
EcoO109I RGGNCCY 1 cut(s) 713
EcoRI GAATTC 1 cut(s) 768
EcoRII CCWGG 7 cut(s) 30, 224, 619, 1282, 1994, 2560, 2783
EcoRV GATATC 1 cut(s) 2021
EcoT14I CCWWGG 4 cut(s) 425, 1246, 1262, 2959
EcoT22I ATGCAT 1 cut(s) 1888
EcoT38I GRGCYC 4 cut(s) 847, 2362, 2858, 3110
ErhI CCWWGG 4 cut(s) 425, 1246, 1262, 2959
Esp3I CGTCTC 1 cut(s) 3078
FaqI GGGAC 9 cut(s) 962, 968, 1059, 1188, 1571, 2105, 2144, 2987, 3016
FauI CCCGC 1 cut(s) 209
FauNDI CATATG 1 cut(s) 916
FbaI TGATCA 2 cut(s) 507, 1864
FblI GTMKAC 2 cut(s) 1577, 1854
FokI GGATG 8 cut(s) 356, 457, 628, 1279, 1953, 2063, 2823, 2830
FriOI GRGCYC 4 cut(s) 847, 2362, 2858, 3110
FspBI CTAG 8 cut(s) 737, 848, 1227, 1590, 1736, 2156, 2414, 2775
GlaI GCGC 5 cut(s) 218, 248, 973, 2428, 2822
GsuI CTGGAG 4 cut(s) 53, 208, 2376, 2947
HaeII RGCGCY 1 cut(s) 975
HaeIII GGCC 9 cut(s) 108, 817, 900, 913, 930, 1366, 1459, 1969, 2783
HapII CCGG 9 cut(s) 51, 86, 175, 529, 1222, 1682, 1814, 2045, 2273
HgaI GACGC 2 cut(s) 91, 1056
HhaI GCGC 5 cut(s) 219, 249, 974, 2429, 2823
Hin1I GRCGYC 2 cut(s) 83, 1048
Hin6I GCGC 5 cut(s) 217, 247, 972, 2427, 2821
HinP1I GCGC 5 cut(s) 217, 247, 972, 2427, 2821
HincII GTYRAC 3 cut(s) 1819, 1855, 1924
HindII GTYRAC 3 cut(s) 1819, 1855, 1924
HindIII AAGCTT 1 cut(s) 1199
HpaII CCGG 9 cut(s) 51, 86, 175, 529, 1222, 1682, 1814, 2045, 2273
HphI GGTGA 7 cut(s) 100, 401, 698, 737, 743, 893, 2699
Hpy99I CGWCG 3 cut(s) 61, 85, 137
HpyAV CCTTC 7 cut(s) 330, 371, 379, 1377, 2083, 2580, 2636
HpyCH4III ACNGT 4 cut(s) 953, 1606, 2028, 2479
HpyCH4IV ACGT 5 cut(s) 179, 2094, 2308, 2952, 3078
HpyF10VI GCNNNNNNNGC 9 cut(s) 253, 910, 1039, 1666, 2465, 2544, 2829, 2894, 3143
HpySE526I ACGT 5 cut(s) 179, 2094, 2308, 2952, 3078
Hsp92I GRCGYC 2 cut(s) 83, 1048
HspAI GCGC 5 cut(s) 217, 247, 972, 2427, 2821
Kpn2I TCCGGA 2 cut(s) 1681, 2044
Ksp22I TGATCA 2 cut(s) 507, 1864
LguI GCTCTTC 3 cut(s) 966, 1101, 3045
MaeI CTAG 8 cut(s) 737, 848, 1227, 1590, 1736, 2156, 2414, 2775
MaeII ACGT 5 cut(s) 179, 2094, 2308, 2952, 3078
MaeIII GTNAC 5 cut(s) 145, 785, 1452, 2161, 2613
MbiI CCGCTC 1 cut(s) 2881
MfeI CAATTG 1 cut(s) 1875
MflI RGATCY 5 cut(s) 637, 1462, 2599, 2702, 2770
MhlI GDGCHC 8 cut(s) 607, 847, 1222, 1834, 2362, 2858, 2899, 3110
MlsI TGGCCA 2 cut(s) 913, 930
MluNI TGGCCA 2 cut(s) 913, 930
MlyI GAGTC 5 cut(s) 170, 415, 551, 2078, 2998
MmeI TCCRAC 5 cut(s) 143, 261, 342, 2733, 3164
Mox20I TGGCCA 2 cut(s) 913, 930
Mph1103I ATGCAT 1 cut(s) 1888
MroI TCCGGA 2 cut(s) 1681, 2044
MroXI GAANNNNTTC 1 cut(s) 3043
MscI TGGCCA 2 cut(s) 913, 930
MseI TTAA 9 cut(s) 114, 861, 1836, 2303, 2400, 2648, 2672, 2762, 2801
MslI CAYNNNNRTG 2 cut(s) 2951, 3020
Msp20I TGGCCA 2 cut(s) 913, 930
MspA1I CMGCKG 6 cut(s) 301, 442, 750, 1345, 2063, 3146
MspI CCGG 9 cut(s) 51, 86, 175, 529, 1222, 1682, 1814, 2045, 2273
MunI CAATTG 1 cut(s) 1875
Mva1269I GAATGC 2 cut(s) 1511, 2871
MvaI CCWGG 7 cut(s) 32, 226, 621, 1284, 1996, 2562, 2785
MvnI CGCG 1 cut(s) 2823
MwoI GCNNNNNNNGC 9 cut(s) 253, 910, 1039, 1666, 2465, 2544, 2829, 2894, 3143
NciI CCSGG 4 cut(s) 52, 530, 1222, 1223
NcoI CCATGG 1 cut(s) 1246
NdeI CATATG 1 cut(s) 916
NlaIV GGNNCC 3 cut(s) 818, 1743, 2772
NmuCI GTSAC 4 cut(s) 785, 1452, 2161, 2613
NsiI ATGCAT 1 cut(s) 1888
NspI RCATGY 2 cut(s) 361, 2950
PaeR7I CTCGAG 2 cut(s) 230, 2892
PagI TCATGA 2 cut(s) 1861, 3021
PciI ACATGT 2 cut(s) 357, 2946
PciSI GCTCTTC 3 cut(s) 966, 1101, 3045
PcsI WCGNNNNNNNCGW 1 cut(s) 3084
PctI GAATGC 2 cut(s) 1511, 2871
PdmI GAANNNNTTC 1 cut(s) 3043
PfeI GAWTC 7 cut(s) 91, 1413, 2081, 2150, 2234, 2417, 2503
PflMI CCANNNNNTGG 1 cut(s) 445
PinAI ACCGGT 1 cut(s) 1813
PleI GAGTC 5 cut(s) 169, 415, 551, 2077, 2998
PpsI GAGTC 5 cut(s) 169, 415, 551, 2077, 2998
Ppu21I YACGTR 2 cut(s) 2953, 3079
PpuMI RGGWCCY 1 cut(s) 713
PscI ACATGT 2 cut(s) 357, 2946
PsiI TTATAA 2 cut(s) 1037, 1724
Psp124BI GAGCTC 3 cut(s) 847, 2362, 2858
Psp5II RGGWCCY 1 cut(s) 713
Psp6I CCWGG 7 cut(s) 30, 224, 619, 1282, 1994, 2560, 2783
PspGI CCWGG 7 cut(s) 30, 224, 619, 1282, 1994, 2560, 2783
PspN4I GGNNCC 3 cut(s) 818, 1743, 2772
PspPI GGNCC 7 cut(s) 713, 779, 816, 1309, 1364, 1811, 2240
PspPPI RGGWCCY 1 cut(s) 713
PstI CTGCAG 5 cut(s) 414, 712, 1129, 1931, 2852
PstNI CAGNNNCTG 2 cut(s) 716, 2237
PsuI RGATCY 5 cut(s) 637, 1462, 2599, 2702, 2770
PvuII CAGCTG 3 cut(s) 1345, 2063, 3146
RsaI GTAC 7 cut(s) 173, 810, 1147, 2566, 2846, 2922, 2951
RsaNI GTAC 7 cut(s) 172, 809, 1146, 2565, 2845, 2921, 2950
RseI CAYNNNNRTG 2 cut(s) 2951, 3020
SacI GAGCTC 3 cut(s) 847, 2362, 2858
SalI GTCGAC 1 cut(s) 1853
SapI GCTCTTC 3 cut(s) 966, 1101, 3045
SaqAI TTAA 9 cut(s) 114, 861, 1836, 2303, 2400, 2648, 2672, 2762, 2801
Sau96I GGNCC 7 cut(s) 713, 779, 816, 1309, 1364, 1811, 2240
ScaI AGTACT 1 cut(s) 810
SchI GAGTC 5 cut(s) 170, 415, 551, 2078, 2998
SduI GDGCHC 8 cut(s) 607, 847, 1222, 1834, 2362, 2858, 2899, 3110
SfcI CTRYAG 6 cut(s) 410, 708, 1125, 1927, 2626, 2848
Sfr274I CTCGAG 2 cut(s) 230, 2892
SgrAI CRCCGGYG 1 cut(s) 85
SinI GGWCC 5 cut(s) 713, 779, 1309, 1811, 2240
SlaI CTCGAG 2 cut(s) 230, 2892
SmaI CCCGGG 1 cut(s) 1223
SmiMI CAYNNNNRTG 2 cut(s) 2951, 3020
SmlI CTYRAG 3 cut(s) 230, 1202, 2892
SmoI CTYRAG 3 cut(s) 230, 1202, 2892
SpeI ACTAGT 1 cut(s) 1735
SrfI GCCCGGGC 1 cut(s) 1223
SsiI CCGC 9 cut(s) 5, 202, 301, 313, 442, 750, 2011, 2823, 2879
SspI AATATT 1 cut(s) 2397
SspMI CTAG 8 cut(s) 737, 848, 1227, 1590, 1736, 2156, 2414, 2775
SstI GAGCTC 3 cut(s) 847, 2362, 2858
StyI CCWWGG 4 cut(s) 425, 1246, 1262, 2959
TaaI ACNGT 4 cut(s) 953, 1606, 2028, 2479
TaiI ACGT 5 cut(s) 182, 2097, 2311, 2955, 3081
TaqI TCGA 9 cut(s) 45, 135, 231, 553, 1026, 1161, 1620, 1854, 2893
TatI WGTACW 3 cut(s) 808, 1145, 2844
TauI GCSGC 1 cut(s) 2013
TfiI GAWTC 7 cut(s) 91, 1413, 2081, 2150, 2234, 2417, 2503
Tru1I TTAA 9 cut(s) 114, 861, 1836, 2303, 2400, 2648, 2672, 2762, 2801
Tru9I TTAA 9 cut(s) 114, 861, 1836, 2303, 2400, 2648, 2672, 2762, 2801
TscAI CASTG 7 cut(s) 419, 958, 1545, 1982, 2033, 2148, 2545
TseFI GTSAC 4 cut(s) 785, 1452, 2161, 2613
Tsp45I GTSAC 4 cut(s) 785, 1452, 2161, 2613
TspDTI ATGAA 7 cut(s) 914, 1246, 2067, 2163, 2237, 3000, 3038
TspGWI ACGGA 2 cut(s) 365, 2274
TspMI CCCGGG 1 cut(s) 1221
TspRI CASTG 7 cut(s) 419, 958, 1545, 1982, 2033, 2148, 2545
Van91I CCANNNNNTGG 1 cut(s) 445
VpaK11BI GGWCC 5 cut(s) 713, 779, 1309, 1811, 2240
XagI CCTNNNNNAGG 1 cut(s) 1055
XapI RAATTY 4 cut(s) 768, 830, 1912, 2695
XbaI TCTAGA 1 cut(s) 2155
XceI RCATGY 2 cut(s) 361, 2950
XcmI CCANNNNNNNNNTGG 1 cut(s) 1349
XhoI CTCGAG 2 cut(s) 230, 2892
XmaI CCCGGG 1 cut(s) 1221
XmiI GTMKAC 2 cut(s) 1577, 1854
XmnI GAANNNNTTC 1 cut(s) 3043
XspI CTAG 8 cut(s) 737, 848, 1227, 1590, 1736, 2156, 2414, 2775
ZrmI AGTACT 1 cut(s) 810
Zsp2I ATGCAT 1 cut(s) 1888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.