RchiOBHm_Chr4g0414151

Belongs to the glycosyltransferase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0011248
Physical Location & Seq
Forward (+)
38547315 .. 38553579
6265 bp
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UTR
Exon/CDS
Intron
PRQ38459

Sequence Viewer

Length: 3204 bp
ATGGCGGGAAATGAGTGGATCAATGGCTACTTGGAGGCGATACTTGACACCGGGGCGACGGCGATTGAGGAGCAGAAGCCGACGCCGGTGAATCTGAGAGAGGGAGGACATTTTAATCCGACTAAGTATTTTGTGGAGGAGGTAGTTACTGGGGTGGATGAGTCTGATTTGTACCGGACGTGGATCAAGGTGGTTGCTACCCGCAACACACGAGAGCGTAGCTCCAGGCTCGAGAACATGTGCTGGCGCATTTGGCATCTTACCCGTAAGAAGAAACAGTTGGAGTGGGAGGAACTTCAACGGTCGGCAAACCGCAGATGGGAGAGGGAACAAGGACGGAGGGATGCAACCGAAGACATGTCAGAAGACTTATCTGAAGGAGAGAAGGGTGATGCTTTGGCAGAGATGCTGCTATGTGAGACTCCAAGAAGAAAGTTCCAGCGAATGGTTTCTAATTTGGAAGTATGGTCAGATGATAAAAAGGAAAAGAAACTATATGTCGTTCTGATCAGTTTGCATGGTTTAGTCCGGGGAGAAAACATGGAGCTTGGTCGAGACTCAGATACAGGTGGACAGGTCAAATATGTGGTAGAGCTTGCCAGGGCACTTGCAAGGATGCCTGGGGTTTATAGGGTAGATCTCTTTACTCGCCAAGTGTCATCTCCGGAGGTTGATTGGAGCTATGGTGAACCAACAGAGATGATAACTGCAGGTCCTGAAGATGGTGATGGTGATCTAGGAGAAAGCAGCGGAGCATATATAATCAGAATTCCTTTTGGTCCTCGCGACCAATACCTCAGTAAAGAAGTACTCTGGCCCCATATTCAAGAATTTGTGGATGGGGCTCTAGCTCACATTCTTAATATGTCCAAAGTTTTGGGTGAACAAATTGGGAGAGGCCAGCCTGTCTGGCCATATGTAATTCATGGCCACTATGCAGATGCAGGGGATAGTGCTGCTCTTCTTTCTGGTGCTTTGAATGTCCCAATGGTTCTAACAGGACACTCACTTGGAAGAAACAAGCTTGAACAGCTTATTAAGCAGGGACGACAATCAAAGGAAGACATTAATTCAACATATAAGATTATGAGAAGGATAGAGGCGGAAGAGCTTTCCCTTGATGCTGCAGAACTTGTTATTACAAGTACAAAACAAGAAATCGAAGAGCAATGGGGACTTTATGATGGGTTTGATGTCAAGCTTGAGAAAGTTTTGCGTGCCCGGGCTAGAAGAGGAGTAAATTGCCATGGTCGTTACATGCCAAGGATGGTGGTTATACCTCCTGGCATGGACTTCAGTAATGTTATGGTCCAAGAAGATGATGCTGATGGGGAACTTAGCCAGCTTATTGGTGGTACAAGTGATGGGTCTTCTTCCCCGAAAGCAATACCAACAATATGGTCAGAAGTGATGAGATTCCTTACAAATCCTCACAAGCCAATGATCTTGGCTCTGTCACGGCCAGATCCAAAGAAGAACATAACCACTCTTTTGAAAGCCTTTGGAGAATGCCGTCCTCTAAGAGAACTTGCTAACCTTACACTGATAATGGGGAATAGGGACTATATAGACGAAATGTCTACCGGAAATGCAAGTGTGCTTACTACAGTGTTGAAAATGATTGATAAGTATGATCTTTATGGGCAAGTGGCCTATCCAAAGCATCACAAGCAATCAGATGTTCCAGATATTTACCGGCTTGCAGCAAAAACAAAGGGAGTTTTCATAAATCCTGCATTAGTGGAGCCTTTTGGGCTAACCTTGATTGAGGCAGCAGCACACGGGCTTCCGATGGTGGCTACAAAAAATGGTGGACCGGTTGACATACATCGGGCGCTTAGTAATGGTTTGCTTGTGGACCCTCATGATCAGCAATCAATTGCTAATGCACTGCTGAAGTTGTTATCGGAAAAGAATTTATGGGTTGACTGCAGAAAGAATGGTTGGAAAAACATACACCTTTTCTCATGGCCTGAACACTGCCGAACCTACTTGACTAGAGTAGCAGCCTGCCGCATGAGACATCCACAGTGGCAGACTGACACCCCGGAGGATGAAATGGCTGCTGAAGAGTCCCTCAATGATTCTCTTAAGGACGTGCAAGATATGTCTCTTAGGCTCTCAGTTGATGGGGACAAATCCTCACTGAATGAATCTCTTGATGTGACTGCAGCTTCTGGTGACCATGAGGTGCAAGATCAAGTGAAGCGTGTCCTAAGCAAGATCAAGAAACCAGATTCTGGTCCTAAAGACCATGAAGAAGGAAATAAGCTGCCGGATAATGTAGCAAGCAAATATCCCTTGTTAAGACGTAGAAGAAAATTGATTGTCATAGCACTCGATTGCTATGACAAATCTGGAGCTCCTGAAAAGAAAATGATTCAAATTGTGCAAGAAATATTTAAGGCTGTAAGACTAGATTCTCAAAGTGCAAGATTTACAGGGTTTGCTCTGCTAACAGCTATGCCAGCATCAGAAACAGTAGAGTTTCTGGCATCAGGGAGGGTACAGGCAAATGAGTTTGATGCGTTGGTTTGTAGCAGTGGAAGTGAGGTTTACTACCCTGGTACTTATACAGAAGAAGATGGAAGACTTTTCCCAGATCCAGATTATTCGTCTCACATTGACTACCGATGGGGTTGTGAAGGATTAAAGAAAACCATTTGGAAGCTCTTAAATGGCCCTGATGGTGAACGGAATTCCACATCTTCGAGCCACATTGAAGAAGATATAAAATCAAGCAGTGCCCATTGCATTTCATACTTGATCAAGGATCCTAGTAAGGCAAGGAAAGTTGATGATTTAAGACAGAAACTCAGGATGCGGGGACTACGCTGCCATCCAATGTACTGCAGGAGCTCAACACGAATGCAAATTGTTCCTCTCCTTGCTTCTCGAGCACAGGCTCTCAGGTACCTTTTCGTACGCTGGAGATTGAATGTTGCAAACATGTATGTGTTCCTTGGAGAAAGTGGAGACACTGATTATGAAGAAATGATAGCAGGGACTCACAAGACCATAATCATGAAAGGGGTGGTGGGAAAGGGTTCTGAAGAGCTACTTAGAACATCAGGAAGCTATCTGAGAGACGACATTGTTCCTCCTCAGAGCCCACTGGTCGCCATTGTAAATGGGCAAGCTCCAACAGCTGATGAGATTGCCACTGCTCTGAAGCAAGTCACCAAATCTGCTGCTGGAATGTGA

Protein Analysis

1067

Amino Acids

119.85

Weight (kDa)

6.1

Isoelectric Point (pI)

46.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GT-B_Sucrose_synth PF00862 166 - 432 2e-11 Sucrose synthase first GT-B domain
Glyco_trans_4_4 PF13579 190 - 388 1e-06 Glycosyl transferase 4-like domain
Glycos_transf_1 PF00534 476 - 648 1e-23 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 480 - 636 2.3e-15 Glycosyl transferases group 1
S6PP PF05116 842 - 1021 7.8e-13 Sucrose-6F-phosphate phosphohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011248)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04920
fragaria_vesca FvH4_4g13520 FvH4_4g13520 FvH4_4g13520
malus_domestica MD04G1013500.v1.1
prunus_persica Prupe.1G159700_v2.0.a1 Prupe.1G159700_v2.0.a1 Prupe.1G159700_v2.0.a1
pyrus_communis pycom04g01050
rosa_chinensis RchiOBHm_Chr4g0414151
rosa_laevigata RLG00000008166
rosa_multiflora Rmu_sc0001063.1_g000015
rosa_roxburghii Rroxscaffold_5G00358160
rosa_rugosa Rorug04G0126600
rosa_samantha Rh4AG184700 Rh4BG184100 Rh4CG196400 Rh4DG180000
rosa_wichuraiana Rw0G005040 Rw4G015730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 701
Acc65I GGTACC 1 cut(s) 2913
AccB1I GGYRCC 1 cut(s) 2913
AccB7I CCANNNNNTGG 2 cut(s) 445, 2240
AccI GTMKAC 1 cut(s) 1580
AccII CGCG 1 cut(s) 786
AccIII TCCGGA 1 cut(s) 664
AciI CCGC 7 cut(s) 5, 202, 313, 750, 1103, 2014, 2824
AclWI GGATC 6 cut(s) 26, 191, 1460, 2597, 2768, 2781
AcoI YGGCCR 3 cut(s) 911, 928, 1460
AcsI RAATTY 4 cut(s) 768, 830, 1915, 2698
AcuI CTGAAG 7 cut(s) 396, 738, 1279, 1916, 2088, 3072, 3191
AcyI GRCGYC 1 cut(s) 83
AfaI GTAC 9 cut(s) 173, 810, 1147, 1357, 2508, 2569, 2849, 2915, 2925
AfiI CCNNNNNNNGG 4 cut(s) 319, 445, 722, 2240
AflII CTTAAG 1 cut(s) 2090
AflIII ACRYGT 3 cut(s) 237, 357, 2949
AgeI ACCGGT 1 cut(s) 1816
AhdI GACNNNNNGTC 1 cut(s) 1576
AjiI CACGTC 2 cut(s) 180, 2098
AjnI CCWGG 5 cut(s) 224, 599, 619, 1282, 2563
AjuI GAANNNNNNNTTGG 4 cut(s) 263, 295, 1927, 1959
Alw21I GWGCWC 3 cut(s) 2365, 2861, 2902
Alw26I GTCTC 7 cut(s) 413, 549, 2014, 2115, 2622, 2970, 3081
AlwI GGATC 6 cut(s) 26, 191, 1460, 2597, 2768, 2781
AlwNI CAGNNNCTG 3 cut(s) 716, 2177, 2240
Ama87I CYCGRG 3 cut(s) 230, 1221, 2895
Aor13HI TCCGGA 1 cut(s) 664
AoxI GGCC 8 cut(s) 815, 898, 911, 928, 1460, 1650, 1970, 2680
ApoI RAATTY 4 cut(s) 768, 830, 1915, 2698
AseI ATTAAT 1 cut(s) 1068
AsiGI ACCGGT 1 cut(s) 1816
Asp700I GAANNNNTTC 2 cut(s) 448, 3046
Asp718I GGTACC 1 cut(s) 2913
AspLEI GCGC 2 cut(s) 249, 1837
AspS9I GGNCC 8 cut(s) 713, 779, 816, 1309, 1814, 1858, 2243, 2681
AsuC2I CCSGG 5 cut(s) 52, 530, 1222, 1223, 2048
AsuHPI GGTGA 9 cut(s) 100, 401, 698, 737, 743, 893, 2192, 2702, 3172
AvaI CYCGRG 3 cut(s) 230, 1221, 2895
AvaII GGWCC 6 cut(s) 713, 779, 1309, 1814, 1858, 2243
BaeGI GKGCMC 3 cut(s) 607, 1222, 2749
BalI TGGCCA 2 cut(s) 913, 930
BamHI GGATCC 1 cut(s) 2773
BanI GGYRCC 1 cut(s) 2913
BanII GRGCYC 4 cut(s) 847, 2365, 2861, 3113
BauI CACGAG 1 cut(s) 210
BbsI GAAGAC 5 cut(s) 360, 372, 1068, 1362, 2596
Bbv12I GWGCWC 3 cut(s) 2365, 2861, 2902
BceAI ACGGC 3 cut(s) 75, 1475, 1497
BciT130I CCWGG 5 cut(s) 226, 601, 621, 1284, 2565
BclI TGATCA 3 cut(s) 507, 1867, 2766
BcnI CCSGG 5 cut(s) 52, 530, 1222, 1223, 2048
BcoDI GTCTC 7 cut(s) 413, 549, 2014, 2115, 2622, 2970, 3081
BfaI CTAG 6 cut(s) 737, 848, 1227, 1998, 2417, 2778
BfmI CTRYAG 6 cut(s) 708, 1125, 1605, 1930, 2169, 2851
BfoI RGCGCY 1 cut(s) 1838
BfrI CTTAAG 1 cut(s) 2090
BfuAI ACCTGC 1 cut(s) 701
BglI GCCNNNNNGGC 2 cut(s) 910, 1753
BglII AGATCT 1 cut(s) 637
BmcAI AGTACT 1 cut(s) 810
Bme18I GGWCC 6 cut(s) 713, 779, 1309, 1814, 1858, 2243
BmeRI GACNNNNNGTC 1 cut(s) 1576
BmeT110I CYCGRG 3 cut(s) 230, 1221, 2895
BmgBI CACGTC 2 cut(s) 180, 2098
BmgT120I GGNCC 8 cut(s) 713, 779, 816, 1309, 1814, 1858, 2243, 2681
BmiI GGNNCC 5 cut(s) 818, 1746, 1860, 2775, 2915
BmrI ACTGGG 1 cut(s) 159
BmuI ACTGGG 1 cut(s) 159
BpiI GAAGAC 5 cut(s) 360, 372, 1068, 1362, 2596
BplI GAGNNNNNCTC 2 cut(s) 206, 238
BpmI CTGGAG 3 cut(s) 208, 2379, 2950
Bpu10I CCTNAGC 1 cut(s) 2216
BpuEI CTTGAG 1 cut(s) 1223
BpuMI CCSGG 5 cut(s) 52, 530, 1222, 1223, 2048
BsaBI GATNNNNATC 1 cut(s) 732
BsaHI GRCGYC 1 cut(s) 83
BsaWI WCCGGW 4 cut(s) 174, 664, 1583, 1816
BsaXI ACNNNNNCTCC 2 cut(s) 670, 700
Bsc4I CCNNNNNNNGG 4 cut(s) 319, 445, 722, 2240
Bse118I RCCGGY 3 cut(s) 85, 1695, 1816
Bse1I ACTGG 2 cut(s) 154, 3120
Bse3DI GCAATG 2 cut(s) 1175, 2749
Bse8I GATNNNNATC 1 cut(s) 732
BseAI TCCGGA 1 cut(s) 664
BseBI CCWGG 5 cut(s) 226, 601, 621, 1284, 2565
BseGI GGATG 9 cut(s) 163, 349, 621, 844, 1272, 2023, 2059, 2826, 2839
BseJI GATNNNNATC 1 cut(s) 732
BseLI CCNNNNNNNGG 4 cut(s) 319, 445, 722, 2240
BseMI GCAATG 2 cut(s) 1175, 2749
BseMII CTCAG 8 cut(s) 86, 573, 811, 2136, 2830, 2923, 3074, 3119
BseNI ACTGG 2 cut(s) 154, 3120
BseRI GAGGAG 4 cut(s) 83, 152, 1248, 3093
BseSI GKGCMC 3 cut(s) 607, 1222, 2749
Bsh1236I CGCG 1 cut(s) 786
Bsh1285I CGRYCG 1 cut(s) 305
BshFI GGCC 8 cut(s) 817, 900, 913, 930, 1462, 1652, 1972, 2682
BshNI GGYRCC 1 cut(s) 2913
BshTI ACCGGT 1 cut(s) 1816
BsiEI CGRYCG 1 cut(s) 305
BsiHKAI GWGCWC 3 cut(s) 2365, 2861, 2902
BsiHKCI CYCGRG 3 cut(s) 230, 1221, 2895
BsiWI CGTACG 1 cut(s) 2923
BslFI GGGAC 8 cut(s) 968, 1059, 1188, 1574, 2059, 2147, 2841, 3019
BslI CCNNNNNNNGG 4 cut(s) 319, 445, 722, 2240
BsmAI GTCTC 7 cut(s) 413, 549, 2014, 2115, 2622, 2970, 3081
BsmBI CGTCTC 2 cut(s) 2622, 3081
BsmFI GGGAC 8 cut(s) 968, 1059, 1188, 1574, 2059, 2147, 2841, 3019
BsmI GAATGC 2 cut(s) 1514, 2874
BsnI GGCC 8 cut(s) 817, 900, 913, 930, 1462, 1652, 1972, 2682
BsoBI CYCGRG 3 cut(s) 230, 1221, 2895
Bsp1286I GDGCHC 8 cut(s) 607, 847, 1222, 2365, 2749, 2861, 2902, 3113
Bsp13I TCCGGA 1 cut(s) 664
Bsp19I CCATGG 1 cut(s) 1246
Bsp68I TCGCGA 1 cut(s) 786
BspACI CCGC 7 cut(s) 5, 202, 313, 750, 1103, 2014, 2824
BspANI GGCC 8 cut(s) 817, 900, 913, 930, 1462, 1652, 1972, 2682
BspCNI CTCAG 8 cut(s) 87, 572, 810, 2135, 2829, 2922, 3075, 3118
BspEI TCCGGA 1 cut(s) 664
BspFNI CGCG 1 cut(s) 786
BspHI TCATGA 2 cut(s) 1864, 3024
BspLI GGNNCC 5 cut(s) 818, 1746, 1860, 2775, 2915
BspMAI CTGCAG 5 cut(s) 712, 1129, 1934, 2173, 2855
BspMI ACCTGC 1 cut(s) 701
BspPI GGATC 6 cut(s) 26, 191, 1460, 2597, 2768, 2781
BspQI GCTCTTC 4 cut(s) 966, 1101, 1158, 3048
BspT107I GGYRCC 1 cut(s) 2913
BspTI CTTAAG 1 cut(s) 2090
BsrDI GCAATG 2 cut(s) 1175, 2749
BsrFI RCCGGY 3 cut(s) 85, 1695, 1816
BsrI ACTGG 2 cut(s) 154, 3120
BssAI RCCGGY 3 cut(s) 85, 1695, 1816
BssNI GRCGYC 1 cut(s) 83
BssSI CACGAG 1 cut(s) 210
BssT1I CCWWGG 3 cut(s) 1246, 1262, 2962
Bst2BI CACGAG 1 cut(s) 210
Bst2UI CCWGG 5 cut(s) 226, 601, 621, 1284, 2565
Bst4CI ACNGT 5 cut(s) 279, 303, 1609, 2031, 2482
Bst6I CTCTTC 6 cut(s) 966, 1101, 1158, 1225, 2064, 3048
BstACI GRCGYC 1 cut(s) 83
BstAFI CTTAAG 1 cut(s) 2090
BstDSI CCRYGG 1 cut(s) 1246
BstEII GGTNACC 1 cut(s) 2180
BstF5I GGATG 9 cut(s) 163, 349, 621, 844, 1272, 2023, 2059, 2826, 2839
BstFNI CGCG 1 cut(s) 786
BstH2I RGCGCY 1 cut(s) 1838
BstHHI GCGC 2 cut(s) 249, 1837
BstMAI GTCTC 7 cut(s) 413, 549, 2014, 2115, 2622, 2970, 3081
BstMCI CGRYCG 1 cut(s) 305
BstMWI GCNNNNNNNGC 9 cut(s) 253, 910, 1030, 1039, 1669, 1753, 2468, 2897, 3146
BstNI CCWGG 5 cut(s) 226, 601, 621, 1284, 2565
BstNSI RCATGY 4 cut(s) 241, 361, 1261, 2953
BstPI GGTNACC 1 cut(s) 2180
BstSFI CTRYAG 6 cut(s) 708, 1125, 1605, 1930, 2169, 2851
BstSLI GKGCMC 3 cut(s) 607, 1222, 2749
BstUI CGCG 1 cut(s) 786
BstV2I GAAGAC 5 cut(s) 360, 372, 1068, 1362, 2596
BstX2I RGATCY 4 cut(s) 637, 1465, 2602, 2773
BstXI CCANNNNNNTGG 3 cut(s) 877, 1349, 1398
BstYI RGATCY 4 cut(s) 637, 1465, 2602, 2773
BsuRI GGCC 8 cut(s) 817, 900, 913, 930, 1462, 1652, 1972, 2682
BtgI CCRYGG 1 cut(s) 1246
BtrI CACGTC 2 cut(s) 180, 2098
BtsCI GGATG 9 cut(s) 163, 349, 621, 844, 1272, 2023, 2059, 2826, 2839
BtsI GCAGTG 5 cut(s) 1889, 1978, 2548, 2749, 3162
BtuMI TCGCGA 1 cut(s) 786
BveI ACCTGC 1 cut(s) 701
CaiI CAGNNNCTG 3 cut(s) 716, 2177, 2240
CciI TCATGA 2 cut(s) 1864, 3024
CfoI GCGC 2 cut(s) 249, 1837
Cfr10I RCCGGY 3 cut(s) 85, 1695, 1816
Cfr13I GGNCC 8 cut(s) 713, 779, 816, 1309, 1814, 1858, 2243, 2681
Cfr9I CCCGGG 1 cut(s) 1221
CseI GACGC 1 cut(s) 91
Csp6I GTAC 9 cut(s) 172, 809, 1146, 1356, 2507, 2568, 2848, 2914, 2924
CspAI ACCGGT 1 cut(s) 1816
CspCI CAANNNNNGTGG 2 cut(s) 1251, 1286
CviQI GTAC 9 cut(s) 172, 809, 1146, 1356, 2507, 2568, 2848, 2914, 2924
DriI GACNNNNNGTC 1 cut(s) 1576
EaeI YGGCCR 3 cut(s) 911, 928, 1460
Eam1104I CTCTTC 6 cut(s) 966, 1101, 1158, 1225, 2064, 3048
Eam1105I GACNNNNNGTC 1 cut(s) 1576
EarI CTCTTC 6 cut(s) 966, 1101, 1158, 1225, 2064, 3048
EciI GGCGGA 1 cut(s) 1118
Ecl136II GAGCTC 2 cut(s) 2363, 2859
Eco130I CCWWGG 3 cut(s) 1246, 1262, 2962
Eco24I GRGCYC 4 cut(s) 847, 2365, 2861, 3113
Eco47I GGWCC 6 cut(s) 713, 779, 1309, 1814, 1858, 2243
Eco53kI GAGCTC 2 cut(s) 2363, 2859
Eco57I CTGAAG 7 cut(s) 396, 738, 1279, 1916, 2088, 3072, 3191
Eco88I CYCGRG 3 cut(s) 230, 1221, 2895
Eco91I GGTNACC 1 cut(s) 2180
EcoICRI GAGCTC 2 cut(s) 2363, 2859
EcoO109I RGGNCCY 1 cut(s) 713
EcoO65I GGTNACC 1 cut(s) 2180
EcoRI GAATTC 2 cut(s) 768, 2698
EcoRII CCWGG 5 cut(s) 224, 599, 619, 1282, 2563
EcoT14I CCWWGG 3 cut(s) 1246, 1262, 2962
EcoT38I GRGCYC 4 cut(s) 847, 2365, 2861, 3113
ErhI CCWWGG 3 cut(s) 1246, 1262, 2962
Esp3I CGTCTC 2 cut(s) 2622, 3081
FalI AAGNNNNNCTT 2 cut(s) 3045, 3077
FaqI GGGAC 8 cut(s) 968, 1059, 1188, 1574, 2059, 2147, 2841, 3019
FauI CCCGC 2 cut(s) 209, 2817
FauNDI CATATG 1 cut(s) 916
FbaI TGATCA 3 cut(s) 507, 1867, 2766
FblI GTMKAC 1 cut(s) 1580
FokI GGATG 9 cut(s) 170, 356, 628, 851, 1279, 2010, 2066, 2826, 2833
FriOI GRGCYC 4 cut(s) 847, 2365, 2861, 3113
FspBI CTAG 6 cut(s) 737, 848, 1227, 1998, 2417, 2778
GlaI GCGC 2 cut(s) 248, 1836
GsuI CTGGAG 3 cut(s) 208, 2379, 2950
HaeII RGCGCY 1 cut(s) 1838
HaeIII GGCC 8 cut(s) 817, 900, 913, 930, 1462, 1652, 1972, 2682
HgaI GACGC 1 cut(s) 91
HhaI GCGC 2 cut(s) 249, 1837
Hin1I GRCGYC 1 cut(s) 83
Hin6I GCGC 2 cut(s) 247, 1835
HinP1I GCGC 2 cut(s) 247, 1835
HincII GTYRAC 2 cut(s) 1822, 1927
HindII GTYRAC 2 cut(s) 1822, 1927
HindIII AAGCTT 2 cut(s) 1022, 1199
HphI GGTGA 9 cut(s) 100, 401, 698, 737, 743, 893, 2192, 2702, 3172
Hpy99I CGWCG 2 cut(s) 61, 85
HpyAV CCTTC 5 cut(s) 371, 379, 1086, 2255, 2639
HpyCH4III ACNGT 5 cut(s) 279, 303, 1609, 2031, 2482
HpyCH4IV ACGT 3 cut(s) 179, 2097, 2311
HpyF10VI GCNNNNNNNGC 9 cut(s) 253, 910, 1030, 1039, 1669, 1753, 2468, 2897, 3146
HpySE526I ACGT 3 cut(s) 179, 2097, 2311
Hsp92I GRCGYC 1 cut(s) 83
HspAI GCGC 2 cut(s) 247, 1835
Kpn2I TCCGGA 1 cut(s) 664
KpnI GGTACC 1 cut(s) 2917
Ksp22I TGATCA 3 cut(s) 507, 1867, 2766
LguI GCTCTTC 4 cut(s) 966, 1101, 1158, 3048
MaeI CTAG 6 cut(s) 737, 848, 1227, 1998, 2417, 2778
MaeII ACGT 3 cut(s) 179, 2097, 2311
MaeIII GTNAC 6 cut(s) 145, 1253, 1455, 2164, 2180, 3178
MfeI CAATTG 1 cut(s) 1878
MflI RGATCY 4 cut(s) 637, 1465, 2602, 2773
MhlI GDGCHC 8 cut(s) 607, 847, 1222, 2365, 2749, 2861, 2902, 3113
MlsI TGGCCA 2 cut(s) 913, 930
MluNI TGGCCA 2 cut(s) 913, 930
MlyI GAGTC 5 cut(s) 170, 415, 551, 2081, 3001
MmeI TCCRAC 4 cut(s) 143, 261, 1925, 3167
Mox20I TGGCCA 2 cut(s) 913, 930
MroI TCCGGA 1 cut(s) 664
MroXI GAANNNNTTC 2 cut(s) 448, 3046
MscI TGGCCA 2 cut(s) 913, 930
MslI CAYNNNNRTG 2 cut(s) 2954, 3023
Msp20I TGGCCA 2 cut(s) 913, 930
MspA1I CMGCKG 2 cut(s) 750, 3149
MspCI CTTAAG 1 cut(s) 2090
MunI CAATTG 1 cut(s) 1878
Mva1269I GAATGC 2 cut(s) 1514, 2874
MvaI CCWGG 5 cut(s) 226, 601, 621, 1284, 2565
MvnI CGCG 1 cut(s) 786
MwoI GCNNNNNNNGC 9 cut(s) 253, 910, 1030, 1039, 1669, 1753, 2468, 2897, 3146
NciI CCSGG 5 cut(s) 52, 530, 1222, 1223, 2048
NcoI CCATGG 1 cut(s) 1246
NdeI CATATG 1 cut(s) 916
NlaIV GGNNCC 5 cut(s) 818, 1746, 1860, 2775, 2915
NmuCI GTSAC 4 cut(s) 1455, 2164, 2180, 3178
NruI TCGCGA 1 cut(s) 786
NspI RCATGY 4 cut(s) 241, 361, 1261, 2953
PaeR7I CTCGAG 2 cut(s) 230, 2895
PagI TCATGA 2 cut(s) 1864, 3024
PciI ACATGT 3 cut(s) 237, 357, 2949
PciSI GCTCTTC 4 cut(s) 966, 1101, 1158, 3048
PctI GAATGC 2 cut(s) 1514, 2874
PdmI GAANNNNTTC 2 cut(s) 448, 3046
PfeI GAWTC 7 cut(s) 91, 1416, 2084, 2153, 2237, 2380, 2420
Pfl23II CGTACG 1 cut(s) 2923
PflMI CCANNNNNTGG 2 cut(s) 445, 2240
PinAI ACCGGT 1 cut(s) 1816
PleI GAGTC 5 cut(s) 169, 415, 551, 2080, 3001
PpsI GAGTC 5 cut(s) 169, 415, 551, 2080, 3001
PpuMI RGGWCCY 1 cut(s) 713
PscI ACATGT 3 cut(s) 237, 357, 2949
PshBI ATTAAT 1 cut(s) 1068
Psp124BI GAGCTC 2 cut(s) 2365, 2861
Psp5II RGGWCCY 1 cut(s) 713
Psp6I CCWGG 5 cut(s) 224, 599, 619, 1282, 2563
PspEI GGTNACC 1 cut(s) 2180
PspGI CCWGG 5 cut(s) 224, 599, 619, 1282, 2563
PspLI CGTACG 1 cut(s) 2923
PspN4I GGNNCC 5 cut(s) 818, 1746, 1860, 2775, 2915
PspPI GGNCC 8 cut(s) 713, 779, 816, 1309, 1814, 1858, 2243, 2681
PspPPI RGGWCCY 1 cut(s) 713
PstI CTGCAG 5 cut(s) 712, 1129, 1934, 2173, 2855
PstNI CAGNNNCTG 3 cut(s) 716, 2177, 2240
PsuI RGATCY 4 cut(s) 637, 1465, 2602, 2773
PvuII CAGCTG 1 cut(s) 3149
RruI TCGCGA 1 cut(s) 786
RsaI GTAC 9 cut(s) 173, 810, 1147, 1357, 2508, 2569, 2849, 2915, 2925
RsaNI GTAC 9 cut(s) 172, 809, 1146, 1356, 2507, 2568, 2848, 2914, 2924
RseI CAYNNNNRTG 2 cut(s) 2954, 3023
SacI GAGCTC 2 cut(s) 2365, 2861
SapI GCTCTTC 4 cut(s) 966, 1101, 1158, 3048
Sau96I GGNCC 8 cut(s) 713, 779, 816, 1309, 1814, 1858, 2243, 2681
ScaI AGTACT 1 cut(s) 810
SchI GAGTC 5 cut(s) 170, 415, 551, 2081, 3001
SduI GDGCHC 8 cut(s) 607, 847, 1222, 2365, 2749, 2861, 2902, 3113
SfcI CTRYAG 6 cut(s) 708, 1125, 1605, 1930, 2169, 2851
Sfr274I CTCGAG 2 cut(s) 230, 2895
SgrAI CRCCGGYG 1 cut(s) 85
SinI GGWCC 6 cut(s) 713, 779, 1309, 1814, 1858, 2243
SlaI CTCGAG 2 cut(s) 230, 2895
SmaI CCCGGG 1 cut(s) 1223
SmiMI CAYNNNNRTG 2 cut(s) 2954, 3023
SmlI CTYRAG 4 cut(s) 230, 1202, 2090, 2895
SmoI CTYRAG 4 cut(s) 230, 1202, 2090, 2895
SrfI GCCCGGGC 1 cut(s) 1223
SsiI CCGC 7 cut(s) 5, 202, 313, 750, 1103, 2014, 2824
SspI AATATT 1 cut(s) 2400
SspMI CTAG 6 cut(s) 737, 848, 1227, 1998, 2417, 2778
SstI GAGCTC 2 cut(s) 2365, 2861
StyI CCWWGG 3 cut(s) 1246, 1262, 2962
TaaI ACNGT 5 cut(s) 279, 303, 1609, 2031, 2482
TaiI ACGT 3 cut(s) 182, 2100, 2314
TaqI TCGA 6 cut(s) 231, 553, 1161, 2340, 2711, 2896
TatI WGTACW 3 cut(s) 808, 1145, 2847
TauI GCSGC 1 cut(s) 2016
TfiI GAWTC 7 cut(s) 91, 1416, 2084, 2153, 2237, 2380, 2420
TseFI GTSAC 4 cut(s) 1455, 2164, 2180, 3178
Tsp45I GTSAC 4 cut(s) 1455, 2164, 2180, 3178
TspDTI ATGAA 8 cut(s) 914, 1714, 2070, 2166, 2271, 2748, 3003, 3041
TspGWI ACGGA 2 cut(s) 352, 2710
TspMI CCCGGG 1 cut(s) 1221
Van91I CCANNNNNTGG 2 cut(s) 445, 2240
Vha464I CTTAAG 1 cut(s) 2090
VpaK11BI GGWCC 6 cut(s) 713, 779, 1309, 1814, 1858, 2243
VspI ATTAAT 1 cut(s) 1068
XapI RAATTY 4 cut(s) 768, 830, 1915, 2698
XceI RCATGY 4 cut(s) 241, 361, 1261, 2953
XcmI CCANNNNNNNNNTGG 1 cut(s) 1349
XhoI CTCGAG 2 cut(s) 230, 2895
XmaI CCCGGG 1 cut(s) 1221
XmiI GTMKAC 1 cut(s) 1580
XmnI GAANNNNTTC 2 cut(s) 448, 3046
XspI CTAG 6 cut(s) 737, 848, 1227, 1998, 2417, 2778
ZrmI AGTACT 1 cut(s) 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.