Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0011248

Physical Location & Seq

Genomic Coordinates Forward (+)

32257162 .. 32264176

Gene Structure 7015 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG184100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2931 bp

ATGCAGTTGGAGTGGGAGGAACTTCAACGGTCGGCAAACCGCAGATGGGAGCGGGAACAAGGACGGAGGGATGCAACCGAAGACATGTCAGAAGACTTGTCCGAAGGAGAGAAGGGTGATGCTTTGGCGGAGATGCTGCTATGTGAGACTCCAAGAAGAAAGTTCCAGCGAATGGTTTCTAATTTGGAAGTATGGTCAGATGATAAAAAGGAAAAGAAACTATATGTCGTTCTGATCAGTTTGCATGGTTTAGTCCGGGGAGAAAACATGGAGCTTGGTCGAGACTCAGATACAGGTGGACAGGTCAAATATGTGGTAGAGCTTGCCAGAGCACTTGCAAGGATGCCTGGGGTTTATAGGGTAGATCTCTTTACTCGCCAAGTGTCATCTCCGGAGGTTGATTGGAGCTATGGTGAACCAACAGAGATGATAACTGCAGGTCCTGAAGATGGTGATGGTGATCTAGGAGAAAGCAGCGGAGCATATATAATCAGAATTCCTTTTGGTCCTCGCGACCAATACCTCAGTAAAGAAGTACTCTGGCCCCATATTCAAGAATTTGTGGATGGGGCTCTAGCTCACATTCTTAATATGTCCAAAGTTTTGGGTGAACAAATTGGGAGAGGCCAGCCTGTCTGGCCATATGTAATTCATGGCCACTATGCAGATGCAGGGGATAGTGCTGCTCTTCTTTCTGGTGCTTTGAATGTCCCGATGGTTCTAACAGGACACTCACTTGGAAGAAACAAGCTTGAACAGCTTATTAAGCAGGGACGACAATCAAAGGAAGACATTAATTCAACATATAAGATTATGAGAAGGATAGAGGCGGAAGAGCTTTCCCTTGATGCTGCAGAACTTGTTATTACAAGTACAAAACAGGAAATCGAAGAGCAATGGGGACTTTATGATGGGTTTGATGTCAAGCTTGAGAAAGTTTTGCGTGCCCGGGCTAGAAGAGGAGTAAATTGCCATGGTCGTTACATGCCAAGGATGGTGGTTATACCTCCTGGCATGGACTTCAGTAATGTTATGGTCCAAGAAGATGATGCTGATGGGGAACTTAGCCAGCTTATTGGCGGTACCAGTGATGGGTCTTCTTCCCCGAAAGCAATACCAACAATCTGGTCAGAAGTGATGAGATTCCTTACAAATCCTCACAAGCCAATGATCTTGGCTCTGTCACGGCCAGATCCAAAGAAGAACATAACCACTCTTTTGAAAGCCTTTGGAGAATGCCGTCCTCTAAGAGAACTTGCTAACCTTACACTGATAATGGGGAATAGGGACTATATAGACGAAATGTCTACCGGAAATGCAAGTGTGCTTACTACAGTGTTGAAAATGATTGATAAGTATGATCTTTATGGGCAAGTGGCCTATCCAAAGCATCACAAGCAATCAGATGTTCCAGATATTTACCGGCTTGCAGCAAAAACAAAGGGAGTTTTCATAAATCCAGCATTAGTGGAGCCTTTTGGGCTAACCTTGATTGAGGCAGCAGCACACGGGCTTCCGATGGTGGCTACAAAAAATGGTGGACCGGTTGACATACATCGGGCGCTTAGTAATGGTTTGCTTGTGGACCCTCATGATCAGCAATCAATTGCTAATGCACTGCTGAAGTTGTTATCGGAAAAGAATTTATGGGTTGAGTGCAGAAAGAATGGTTGGAAAAACATACACCTTTTCTCATGGCCTGAACACTGCCGAACCTACTTGACTAGAGTAGCAGCCTGCCGCATGAGACATCCACAGTGGCAGACTGACACCCCGGAGGATGAAATGGCTGCTGAAGAGTCCCTCAATGATTCTCTTAAGGACGTGCAAGATATGTCTCTTAGGCTCTCAGTTGATGGGGACAAATCCTCACTGAATGAATCTCTTGATGTGACTGCAGCTTCTGGTGACCATGAGGTGCAAGATCAAGTGAAGCGTGTCCTAAGCAAGATCAAGAAACCAGATTCTGGTCCAAAAGACCATGAAGAAGGAAATAAGCTGCCGGATAATGTAGCAAGCAAATATCCCTTGTTAAGACGTAGAAGAAAATTGATTGTCATAGCACTCGATTGCTATGACAAATCTGGAGCTCCTGAAAAGAAAATGATTCAAATTGTGCAAGAAATATTTAAGGCTGTAAGACTAGATTCTCAAAGTGCAAGATTTACAGGGTTTGCTCTGCTAACAGCTATGCCAGCATCAGAAACAGTAGAGTTTCTGGCATCAGGGAGGGTACAGGCAAATGAGTTTGATGCGTTGGTTTGTAGCAGTGGAAGTGAGGTTTACTACCCTGGTACTTATACAGAAGAAGATGGAAGACTTTTCCCAGATCCAGATTATTCGTCACACATTGACTACCGATGGGGTTGTGAAGGATTAAAGAAAACCATTTGGAAGCTCTTAAATGATCCTGATGGTGAACGGAATTCCACATCTTCGAGCCACATTGAAGAAGATATAAAATCAAGCAGTGCCCATTGCATTTCATACTTGATCAAGGATCCTAGTAAGGCAAGGAAAGTTGATGATTTAAGACAGAAACTCAGGATGCGGGGACTACGCTGCCATCCAATGTACTGCAGGAGCTCAACGCGAATGCAAATTGTTCCTCTCCTTGCTTCTCGAGCACAGGCTCTCAGGTACCTTTTCGTACGCTGGAGATTGAATGTCGCAAATATGTATGTGTTCCTTGGAGAAAGTGGAGACACTGATTATGAAGAAATGATAGCAGGGACTCACAAGACCATAATCATGAAAGGGGTGGTGGGAAAGGGTTCTGAAGAGCTGCTAAGAACATCAGGAAGCTATCTGAGAGACGACATTGTTCCTCCTCAGAGCCCACTGGTCGCCATTGTAAATGGGCAAGCTCCAACAGCTGATGAGATTGCCACTGCTCTGAAGCAAGTCACCAAATCTGCTGCTGGAATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0008194 GO:0012505 GO:0016740 GO:0016757 GO:0016758 GO:0035251 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046524 GO:0046527

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 S6PP Sucrose_synth

Protein Analysis

976

Amino Acids

109.32

Weight (kDa)

5.94

Isoelectric Point (pI)

48.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GT-B_Sucrose_synth	PF00862	75 - 341	1.7e-11	Sucrose synthase first GT-B domain
Glyco_trans_4_4	PF13579	99 - 297	8.9e-07	Glycosyl transferase 4-like domain
Glycos_transf_1	PF00534	385 - 557	1.3e-23	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	389 - 545	2e-15	Glycosyl transferases group 1
S6PP	PF05116	751 - 930	6e-13	Sucrose-6F-phosphate phosphohydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011248)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04920
fragaria_vesca	FvH4_4g13520 FvH4_4g13520 FvH4_4g13520
malus_domestica	MD04G1013500.v1.1
prunus_persica	Prupe.1G159700_v2.0.a1 Prupe.1G159700_v2.0.a1 Prupe.1G159700_v2.0.a1
pyrus_communis	pycom04g01050
rosa_chinensis	RchiOBHm_Chr4g0414151
rosa_laevigata	RLG00000008166
rosa_multiflora	Rmu_sc0001063.1_g000015
rosa_roxburghii	Rroxscaffold_5G00358160
rosa_rugosa	Rorug04G0126600
rosa_samantha	Rh4AG184700 Rh4BG184100 Rh4CG196400 Rh4DG180000
rosa_wichuraiana	Rw0G005040 Rw4G015730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	428
Acc65I	GGTACC	2 cut(s)	1082, 2640
AccB1I	GGYRCC	2 cut(s)	1082, 2640
AccB7I	CCANNNNNTGG	2 cut(s)	172, 1967
AccBSI	CCGCTC	1 cut(s)	52
AccI	GTMKAC	1 cut(s)	1307
AccII	CGCG	2 cut(s)	513, 2593
AccIII	TCCGGA	1 cut(s)	391
AciI	CCGC	8 cut(s)	40, 52, 128, 477, 830, 1080, 1741, 2551
AclWI	GGATC	5 cut(s)	1187, 2324, 2402, 2495, 2508
AcoI	YGGCCR	3 cut(s)	638, 655, 1187
AcsI	RAATTY	4 cut(s)	495, 557, 1642, 2425
AcuI	CTGAAG	6 cut(s)	465, 1006, 1643, 1815, 2799, 2918
AfaI	GTAC	8 cut(s)	537, 874, 1084, 2235, 2296, 2576, 2642, 2652
AfiI	CCNNNNNNNGG	5 cut(s)	46, 172, 449, 1092, 1967
AflII	CTTAAG	1 cut(s)	1817
AflIII	ACRYGT	1 cut(s)	84
AgeI	ACCGGT	1 cut(s)	1543
AhdI	GACNNNNNGTC	1 cut(s)	1303
AjiI	CACGTC	1 cut(s)	1825
AjnI	CCWGG	3 cut(s)	346, 1009, 2290
AjuI	GAANNNNNNNTTGG	2 cut(s)	1654, 1686
Alw21I	GWGCWC	4 cut(s)	334, 2092, 2588, 2629
Alw26I	GTCTC	6 cut(s)	140, 276, 1741, 1842, 2697, 2808
AlwI	GGATC	5 cut(s)	1187, 2324, 2402, 2495, 2508
AlwNI	CAGNNNCTG	3 cut(s)	443, 1904, 1967
Ama87I	CYCGRG	2 cut(s)	948, 2622
Aor13HI	TCCGGA	1 cut(s)	391
AoxI	GGCC	7 cut(s)	542, 625, 638, 655, 1187, 1377, 1697
ApoI	RAATTY	4 cut(s)	495, 557, 1642, 2425
AseI	ATTAAT	1 cut(s)	795
AsiGI	ACCGGT	1 cut(s)	1543
Asp700I	GAANNNNTTC	2 cut(s)	175, 2773
Asp718I	GGTACC	2 cut(s)	1082, 2640
AspLEI	GCGC	1 cut(s)	1564
AspS9I	GGNCC	7 cut(s)	440, 506, 543, 1036, 1541, 1585, 1970
AsuC2I	CCSGG	4 cut(s)	257, 949, 950, 1775
AsuHPI	GGTGA	8 cut(s)	128, 425, 464, 470, 620, 1919, 2429, 2899
AvaI	CYCGRG	2 cut(s)	948, 2622
AvaII	GGWCC	6 cut(s)	440, 506, 1036, 1541, 1585, 1970
BaeGI	GKGCMC	2 cut(s)	949, 2476
BalI	TGGCCA	2 cut(s)	640, 657
BamHI	GGATCC	1 cut(s)	2500
BanI	GGYRCC	2 cut(s)	1082, 2640
BanII	GRGCYC	4 cut(s)	574, 2092, 2588, 2840
BbsI	GAAGAC	5 cut(s)	87, 99, 795, 1089, 2323
Bbv12I	GWGCWC	4 cut(s)	334, 2092, 2588, 2629
BceAI	ACGGC	2 cut(s)	1202, 1224
BciT130I	CCWGG	3 cut(s)	348, 1011, 2292
BclI	TGATCA	3 cut(s)	234, 1594, 2493
BcnI	CCSGG	4 cut(s)	257, 949, 950, 1775
BcoDI	GTCTC	6 cut(s)	140, 276, 1741, 1842, 2697, 2808
BfaI	CTAG	6 cut(s)	464, 575, 954, 1725, 2144, 2505
BfmI	CTRYAG	5 cut(s)	435, 852, 1332, 1896, 2578
BfoI	RGCGCY	1 cut(s)	1565
BfrI	CTTAAG	1 cut(s)	1817
BfuAI	ACCTGC	1 cut(s)	428
BglI	GCCNNNNNGGC	2 cut(s)	637, 1480
BglII	AGATCT	1 cut(s)	364
BmcAI	AGTACT	1 cut(s)	537
Bme1390I	CCNGG	7 cut(s)	257, 348, 949, 950, 1011, 1775, 2292
Bme18I	GGWCC	6 cut(s)	440, 506, 1036, 1541, 1585, 1970
BmeRI	GACNNNNNGTC	1 cut(s)	1303
BmeT110I	CYCGRG	2 cut(s)	948, 2622
BmgBI	CACGTC	1 cut(s)	1825
BmgT120I	GGNCC	7 cut(s)	440, 506, 543, 1036, 1541, 1585, 1970
BmiI	GGNNCC	6 cut(s)	545, 1084, 1473, 1587, 2502, 2642
BmrFI	CCNGG	7 cut(s)	257, 348, 949, 950, 1011, 1775, 2292
BpiI	GAAGAC	5 cut(s)	87, 99, 795, 1089, 2323
BpmI	CTGGAG	2 cut(s)	2106, 2677
Bpu10I	CCTNAGC	1 cut(s)	1943
BpuEI	CTTGAG	1 cut(s)	950
BpuMI	CCSGG	4 cut(s)	257, 949, 950, 1775
BsaBI	GATNNNNATC	1 cut(s)	459
BsaJI	CCNNGG	8 cut(s)	256, 347, 948, 973, 989, 1773, 2290, 2689
BsaWI	WCCGGW	3 cut(s)	391, 1310, 1543
BsaXI	ACNNNNNCTCC	2 cut(s)	397, 427
Bsc4I	CCNNNNNNNGG	5 cut(s)	46, 172, 449, 1092, 1967
Bse118I	RCCGGY	2 cut(s)	1422, 1543
Bse1I	ACTGG	2 cut(s)	1086, 2847
Bse3DI	GCAATG	2 cut(s)	902, 2476
Bse8I	GATNNNNATC	1 cut(s)	459
BseAI	TCCGGA	1 cut(s)	391
BseBI	CCWGG	3 cut(s)	348, 1011, 2292
BseDI	CCNNGG	8 cut(s)	256, 347, 948, 973, 989, 1773, 2290, 2689
BseGI	GGATG	8 cut(s)	76, 348, 571, 999, 1750, 1786, 2553, 2566
BseJI	GATNNNNATC	1 cut(s)	459
BseLI	CCNNNNNNNGG	5 cut(s)	46, 172, 449, 1092, 1967
BseMI	GCAATG	2 cut(s)	902, 2476
BseMII	CTCAG	7 cut(s)	300, 538, 1863, 2557, 2650, 2801, 2846
BseNI	ACTGG	2 cut(s)	1086, 2847
BseRI	GAGGAG	2 cut(s)	975, 2820
BseSI	GKGCMC	2 cut(s)	949, 2476
BsgI	GTGCAG	1 cut(s)	1678
Bsh1236I	CGCG	2 cut(s)	513, 2593
Bsh1285I	CGRYCG	1 cut(s)	32
BshFI	GGCC	7 cut(s)	544, 627, 640, 657, 1189, 1379, 1699
BshNI	GGYRCC	2 cut(s)	1082, 2640
BshTI	ACCGGT	1 cut(s)	1543
BsiEI	CGRYCG	1 cut(s)	32
BsiHKAI	GWGCWC	4 cut(s)	334, 2092, 2588, 2629
BsiHKCI	CYCGRG	2 cut(s)	948, 2622
BsiSI	CCGG	8 cut(s)	256, 392, 949, 1311, 1423, 1544, 1775, 2003
BsiWI	CGTACG	1 cut(s)	2650
BslFI	GGGAC	8 cut(s)	695, 786, 915, 1301, 1786, 1874, 2568, 2746
BslI	CCNNNNNNNGG	5 cut(s)	46, 172, 449, 1092, 1967
BsmAI	GTCTC	6 cut(s)	140, 276, 1741, 1842, 2697, 2808
BsmBI	CGTCTC	1 cut(s)	2808
BsmFI	GGGAC	8 cut(s)	695, 786, 915, 1301, 1786, 1874, 2568, 2746
BsmI	GAATGC	2 cut(s)	1241, 2601
BsnI	GGCC	7 cut(s)	544, 627, 640, 657, 1189, 1379, 1699
BsoBI	CYCGRG	2 cut(s)	948, 2622
Bsp1286I	GDGCHC	8 cut(s)	334, 574, 949, 2092, 2476, 2588, 2629, 2840
Bsp13I	TCCGGA	1 cut(s)	391
Bsp19I	CCATGG	1 cut(s)	973
Bsp68I	TCGCGA	1 cut(s)	513
BspACI	CCGC	8 cut(s)	40, 52, 128, 477, 830, 1080, 1741, 2551
BspANI	GGCC	7 cut(s)	544, 627, 640, 657, 1189, 1379, 1699
BspCNI	CTCAG	7 cut(s)	299, 537, 1862, 2556, 2649, 2802, 2845
BspEI	TCCGGA	1 cut(s)	391
BspFNI	CGCG	2 cut(s)	513, 2593
BspHI	TCATGA	2 cut(s)	1591, 2751
BspLI	GGNNCC	6 cut(s)	545, 1084, 1473, 1587, 2502, 2642
BspMAI	CTGCAG	4 cut(s)	439, 856, 1900, 2582
BspMI	ACCTGC	1 cut(s)	428
BspPI	GGATC	5 cut(s)	1187, 2324, 2402, 2495, 2508
BspQI	GCTCTTC	4 cut(s)	693, 828, 885, 2775
BspT107I	GGYRCC	2 cut(s)	1082, 2640
BspTI	CTTAAG	1 cut(s)	1817
BsrBI	CCGCTC	1 cut(s)	52
BsrDI	GCAATG	2 cut(s)	902, 2476
BsrFI	RCCGGY	2 cut(s)	1422, 1543
BsrI	ACTGG	2 cut(s)	1086, 2847
BssAI	RCCGGY	2 cut(s)	1422, 1543
BssECI	CCNNGG	8 cut(s)	256, 347, 948, 973, 989, 1773, 2290, 2689
BssT1I	CCWWGG	3 cut(s)	973, 989, 2689
Bst2UI	CCWGG	3 cut(s)	348, 1011, 2292
Bst4CI	ACNGT	4 cut(s)	30, 1336, 1758, 2209
Bst6I	CTCTTC	6 cut(s)	693, 828, 885, 952, 1791, 2775
BstAFI	CTTAAG	1 cut(s)	1817
BstC8I	GCNNGC	9 cut(s)	324, 629, 945, 1070, 1428, 1738, 2017, 2196, 2865
BstDSI	CCRYGG	1 cut(s)	973
BstEII	GGTNACC	1 cut(s)	1907
BstF5I	GGATG	8 cut(s)	76, 348, 571, 999, 1750, 1786, 2553, 2566
BstFNI	CGCG	2 cut(s)	513, 2593
BstH2I	RGCGCY	1 cut(s)	1565
BstHHI	GCGC	1 cut(s)	1564
BstMAI	GTCTC	6 cut(s)	140, 276, 1741, 1842, 2697, 2808
BstMCI	CGRYCG	1 cut(s)	32
BstMWI	GCNNNNNNNGC	8 cut(s)	637, 757, 766, 1396, 1480, 2195, 2624, 2873
BstNI	CCWGG	3 cut(s)	348, 1011, 2292
BstNSI	RCATGY	2 cut(s)	88, 988
BstPI	GGTNACC	1 cut(s)	1907
BstSCI	CCNGG	7 cut(s)	255, 346, 947, 948, 1009, 1773, 2290
BstSFI	CTRYAG	5 cut(s)	435, 852, 1332, 1896, 2578
BstSLI	GKGCMC	2 cut(s)	949, 2476
BstUI	CGCG	2 cut(s)	513, 2593
BstV2I	GAAGAC	5 cut(s)	87, 99, 795, 1089, 2323
BstX2I	RGATCY	4 cut(s)	364, 1192, 2329, 2500
BstXI	CCANNNNNNTGG	3 cut(s)	604, 1076, 1125
BstYI	RGATCY	4 cut(s)	364, 1192, 2329, 2500
BsuRI	GGCC	7 cut(s)	544, 627, 640, 657, 1189, 1379, 1699
BtgI	CCRYGG	1 cut(s)	973
BtrI	CACGTC	1 cut(s)	1825
BtsCI	GGATG	8 cut(s)	76, 348, 571, 999, 1750, 1786, 2553, 2566
BtsI	GCAGTG	5 cut(s)	1616, 1705, 2275, 2476, 2889
BtuMI	TCGCGA	1 cut(s)	513
BveI	ACCTGC	1 cut(s)	428
Cac8I	GCNNGC	9 cut(s)	324, 629, 945, 1070, 1428, 1738, 2017, 2196, 2865
CaiI	CAGNNNCTG	3 cut(s)	443, 1904, 1967
CciI	TCATGA	2 cut(s)	1591, 2751
CfoI	GCGC	1 cut(s)	1564
Cfr10I	RCCGGY	2 cut(s)	1422, 1543
Cfr13I	GGNCC	7 cut(s)	440, 506, 543, 1036, 1541, 1585, 1970
Cfr9I	CCCGGG	1 cut(s)	948
Csp6I	GTAC	8 cut(s)	536, 873, 1083, 2234, 2295, 2575, 2641, 2651
CspAI	ACCGGT	1 cut(s)	1543
CspCI	CAANNNNNGTGG	2 cut(s)	978, 1013
CviQI	GTAC	8 cut(s)	536, 873, 1083, 2234, 2295, 2575, 2641, 2651
DriI	GACNNNNNGTC	1 cut(s)	1303
EaeI	YGGCCR	3 cut(s)	638, 655, 1187
Eam1104I	CTCTTC	6 cut(s)	693, 828, 885, 952, 1791, 2775
Eam1105I	GACNNNNNGTC	1 cut(s)	1303
EarI	CTCTTC	6 cut(s)	693, 828, 885, 952, 1791, 2775
EciI	GGCGGA	2 cut(s)	143, 845
Ecl136II	GAGCTC	2 cut(s)	2090, 2586
Eco130I	CCWWGG	3 cut(s)	973, 989, 2689
Eco24I	GRGCYC	4 cut(s)	574, 2092, 2588, 2840
Eco47I	GGWCC	6 cut(s)	440, 506, 1036, 1541, 1585, 1970
Eco53kI	GAGCTC	2 cut(s)	2090, 2586
Eco57I	CTGAAG	6 cut(s)	465, 1006, 1643, 1815, 2799, 2918
Eco88I	CYCGRG	2 cut(s)	948, 2622
Eco91I	GGTNACC	1 cut(s)	1907
EcoICRI	GAGCTC	2 cut(s)	2090, 2586
EcoO109I	RGGNCCY	1 cut(s)	440
EcoO65I	GGTNACC	1 cut(s)	1907
EcoRI	GAATTC	2 cut(s)	495, 2425
EcoRII	CCWGG	3 cut(s)	346, 1009, 2290
EcoT14I	CCWWGG	3 cut(s)	973, 989, 2689
EcoT38I	GRGCYC	4 cut(s)	574, 2092, 2588, 2840
ErhI	CCWWGG	3 cut(s)	973, 989, 2689
Esp3I	CGTCTC	1 cut(s)	2808
FaqI	GGGAC	8 cut(s)	695, 786, 915, 1301, 1786, 1874, 2568, 2746
FauI	CCCGC	2 cut(s)	45, 2544
FauNDI	CATATG	1 cut(s)	643
FbaI	TGATCA	3 cut(s)	234, 1594, 2493
FblI	GTMKAC	1 cut(s)	1307
FokI	GGATG	8 cut(s)	83, 355, 578, 1006, 1737, 1793, 2553, 2560
FriOI	GRGCYC	4 cut(s)	574, 2092, 2588, 2840
FspBI	CTAG	6 cut(s)	464, 575, 954, 1725, 2144, 2505
GlaI	GCGC	1 cut(s)	1563
GsuI	CTGGAG	2 cut(s)	2106, 2677
HaeII	RGCGCY	1 cut(s)	1565
HaeIII	GGCC	7 cut(s)	544, 627, 640, 657, 1189, 1379, 1699
HapII	CCGG	8 cut(s)	256, 392, 949, 1311, 1423, 1544, 1775, 2003
HhaI	GCGC	1 cut(s)	1564
Hin6I	GCGC	1 cut(s)	1562
HinP1I	GCGC	1 cut(s)	1562
HincII	GTYRAC	1 cut(s)	1549
HindII	GTYRAC	1 cut(s)	1549
HindIII	AAGCTT	2 cut(s)	749, 926
HpaII	CCGG	8 cut(s)	256, 392, 949, 1311, 1423, 1544, 1775, 2003
HphI	GGTGA	8 cut(s)	128, 425, 464, 470, 620, 1919, 2429, 2899
Hpy166II	GTNNAC	9 cut(s)	299, 416, 611, 1308, 1541, 1549, 1585, 2284, 2420
Hpy8I	GTNNAC	9 cut(s)	299, 416, 611, 1308, 1541, 1549, 1585, 2284, 2420
HpyAV	CCTTC	5 cut(s)	98, 106, 813, 1982, 2366
HpyCH4III	ACNGT	4 cut(s)	30, 1336, 1758, 2209
HpyCH4IV	ACGT	2 cut(s)	1824, 2038
HpyF10VI	GCNNNNNNNGC	8 cut(s)	637, 757, 766, 1396, 1480, 2195, 2624, 2873
HpySE526I	ACGT	2 cut(s)	1824, 2038
HspAI	GCGC	1 cut(s)	1562
Kpn2I	TCCGGA	1 cut(s)	391
KpnI	GGTACC	2 cut(s)	1086, 2644
Ksp22I	TGATCA	3 cut(s)	234, 1594, 2493
LguI	GCTCTTC	4 cut(s)	693, 828, 885, 2775
LmnI	GCTCC	9 cut(s)	49, 271, 405, 479, 1471, 2087, 2095, 2583, 2872
MaeI	CTAG	6 cut(s)	464, 575, 954, 1725, 2144, 2505
MaeII	ACGT	2 cut(s)	1824, 2038
MaeIII	GTNAC	6 cut(s)	980, 1182, 1891, 1907, 2343, 2905
MbiI	CCGCTC	1 cut(s)	52
MfeI	CAATTG	1 cut(s)	1605
MflI	RGATCY	4 cut(s)	364, 1192, 2329, 2500
MhlI	GDGCHC	8 cut(s)	334, 574, 949, 2092, 2476, 2588, 2629, 2840
MlsI	TGGCCA	2 cut(s)	640, 657
MluNI	TGGCCA	2 cut(s)	640, 657
MlyI	GAGTC	4 cut(s)	142, 278, 1808, 2728
MmeI	TCCRAC	2 cut(s)	1652, 2894
Mox20I	TGGCCA	2 cut(s)	640, 657
MroI	TCCGGA	1 cut(s)	391
MroXI	GAANNNNTTC	2 cut(s)	175, 2773
MscI	TGGCCA	2 cut(s)	640, 657
MseI	TTAA	9 cut(s)	588, 765, 795, 1818, 2033, 2130, 2378, 2402, 2531
MslI	CAYNNNNRTG	1 cut(s)	2750
Msp20I	TGGCCA	2 cut(s)	640, 657
MspA1I	CMGCKG	2 cut(s)	477, 2876
MspCI	CTTAAG	1 cut(s)	1817
MspI	CCGG	8 cut(s)	256, 392, 949, 1311, 1423, 1544, 1775, 2003
MspR9I	CCNGG	7 cut(s)	257, 348, 949, 950, 1011, 1775, 2292
MunI	CAATTG	1 cut(s)	1605
Mva1269I	GAATGC	2 cut(s)	1241, 2601
MvaI	CCWGG	3 cut(s)	348, 1011, 2292
MvnI	CGCG	2 cut(s)	513, 2593
MwoI	GCNNNNNNNGC	8 cut(s)	637, 757, 766, 1396, 1480, 2195, 2624, 2873
NciI	CCSGG	4 cut(s)	257, 949, 950, 1775
NcoI	CCATGG	1 cut(s)	973
NdeI	CATATG	1 cut(s)	643
NlaIV	GGNNCC	6 cut(s)	545, 1084, 1473, 1587, 2502, 2642
NmuCI	GTSAC	5 cut(s)	1182, 1891, 1907, 2343, 2905
NruI	TCGCGA	1 cut(s)	513
NspI	RCATGY	2 cut(s)	88, 988
PaeR7I	CTCGAG	1 cut(s)	2622
PagI	TCATGA	2 cut(s)	1591, 2751
PciI	ACATGT	1 cut(s)	84
PciSI	GCTCTTC	4 cut(s)	693, 828, 885, 2775
PctI	GAATGC	2 cut(s)	1241, 2601
PdmI	GAANNNNTTC	2 cut(s)	175, 2773
PfeI	GAWTC	6 cut(s)	1143, 1811, 1880, 1964, 2107, 2147
Pfl23II	CGTACG	1 cut(s)	2650
PflMI	CCANNNNNTGG	2 cut(s)	172, 1967
PinAI	ACCGGT	1 cut(s)	1543
PleI	GAGTC	4 cut(s)	142, 278, 1807, 2728
PpsI	GAGTC	4 cut(s)	142, 278, 1807, 2728
PpuMI	RGGWCCY	1 cut(s)	440
PscI	ACATGT	1 cut(s)	84
PshBI	ATTAAT	1 cut(s)	795
Psp124BI	GAGCTC	2 cut(s)	2092, 2588
Psp5II	RGGWCCY	1 cut(s)	440
Psp6I	CCWGG	3 cut(s)	346, 1009, 2290
PspEI	GGTNACC	1 cut(s)	1907
PspGI	CCWGG	3 cut(s)	346, 1009, 2290
PspLI	CGTACG	1 cut(s)	2650
PspN4I	GGNNCC	6 cut(s)	545, 1084, 1473, 1587, 2502, 2642
PspPI	GGNCC	7 cut(s)	440, 506, 543, 1036, 1541, 1585, 1970
PspPPI	RGGWCCY	1 cut(s)	440
PstI	CTGCAG	4 cut(s)	439, 856, 1900, 2582
PstNI	CAGNNNCTG	3 cut(s)	443, 1904, 1967
PsuI	RGATCY	4 cut(s)	364, 1192, 2329, 2500
PvuII	CAGCTG	1 cut(s)	2876
RruI	TCGCGA	1 cut(s)	513
RsaI	GTAC	8 cut(s)	537, 874, 1084, 2235, 2296, 2576, 2642, 2652
RsaNI	GTAC	8 cut(s)	536, 873, 1083, 2234, 2295, 2575, 2641, 2651
RseI	CAYNNNNRTG	1 cut(s)	2750
SacI	GAGCTC	2 cut(s)	2092, 2588
SapI	GCTCTTC	4 cut(s)	693, 828, 885, 2775
SaqAI	TTAA	9 cut(s)	588, 765, 795, 1818, 2033, 2130, 2378, 2402, 2531
Sau96I	GGNCC	7 cut(s)	440, 506, 543, 1036, 1541, 1585, 1970
ScaI	AGTACT	1 cut(s)	537
SchI	GAGTC	4 cut(s)	142, 278, 1808, 2728
ScrFI	CCNGG	7 cut(s)	257, 348, 949, 950, 1011, 1775, 2292
SduI	GDGCHC	8 cut(s)	334, 574, 949, 2092, 2476, 2588, 2629, 2840
SfcI	CTRYAG	5 cut(s)	435, 852, 1332, 1896, 2578
Sfr274I	CTCGAG	1 cut(s)	2622
SinI	GGWCC	6 cut(s)	440, 506, 1036, 1541, 1585, 1970
SlaI	CTCGAG	1 cut(s)	2622
SmaI	CCCGGG	1 cut(s)	950
SmiMI	CAYNNNNRTG	1 cut(s)	2750
SmlI	CTYRAG	3 cut(s)	929, 1817, 2622
SmoI	CTYRAG	3 cut(s)	929, 1817, 2622
SrfI	GCCCGGGC	1 cut(s)	950
SsiI	CCGC	8 cut(s)	40, 52, 128, 477, 830, 1080, 1741, 2551
SspI	AATATT	1 cut(s)	2127
SspMI	CTAG	6 cut(s)	464, 575, 954, 1725, 2144, 2505
SstI	GAGCTC	2 cut(s)	2092, 2588
StyD4I	CCNGG	7 cut(s)	255, 346, 947, 948, 1009, 1773, 2290
StyI	CCWWGG	3 cut(s)	973, 989, 2689
TaaI	ACNGT	4 cut(s)	30, 1336, 1758, 2209
TaiI	ACGT	2 cut(s)	1827, 2041
TaqI	TCGA	5 cut(s)	280, 888, 2067, 2438, 2623
TatI	WGTACW	3 cut(s)	535, 872, 2574
TauI	GCSGC	1 cut(s)	1743
TfiI	GAWTC	6 cut(s)	1143, 1811, 1880, 1964, 2107, 2147
Tru1I	TTAA	9 cut(s)	588, 765, 795, 1818, 2033, 2130, 2378, 2402, 2531
Tru9I	TTAA	9 cut(s)	588, 765, 795, 1818, 2033, 2130, 2378, 2402, 2531
TseFI	GTSAC	5 cut(s)	1182, 1891, 1907, 2343, 2905
Tsp45I	GTSAC	5 cut(s)	1182, 1891, 1907, 2343, 2905
TspDTI	ATGAA	8 cut(s)	641, 1441, 1797, 1893, 1998, 2475, 2730, 2768
TspGWI	ACGGA	2 cut(s)	79, 2437
TspMI	CCCGGG	1 cut(s)	948
Van91I	CCANNNNNTGG	2 cut(s)	172, 1967
Vha464I	CTTAAG	1 cut(s)	1817
VpaK11BI	GGWCC	6 cut(s)	440, 506, 1036, 1541, 1585, 1970
VspI	ATTAAT	1 cut(s)	795
XapI	RAATTY	4 cut(s)	495, 557, 1642, 2425
XceI	RCATGY	2 cut(s)	88, 988
XhoI	CTCGAG	1 cut(s)	2622
XmaI	CCCGGG	1 cut(s)	948
XmiI	GTMKAC	1 cut(s)	1307
XmnI	GAANNNNTTC	2 cut(s)	175, 2773
XspI	CTAG	6 cut(s)	464, 575, 954, 1725, 2144, 2505
ZrmI	AGTACT	1 cut(s)	537

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.