MD04G1021200.v1.1

Transcription factor IIIC subunit delta N-term

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009196
Physical Location & Seq
Reverse (-)
2497771 .. 2502701
4931 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1021200.v1.1.491

Sequence Viewer

Length: 2571 bp
ATGTCTTTCTTTCCCTCCAATTCCCAAATCCAAACCCAAAACGCCGACTCCCGTTTCCAGGCCACCGTGCTCGTCGGCGCTCCATCCTTCCCCGACGCCATCGCTTGGTCCGACGAGAACCTCATCGCCGTCGCTTCCGGCCACCTCGTCACCATCCTGAATCCGGCTGCTCTGCCTTTTGGACCAAGAGGCCTAATCACCATAAAAAACAGCCAACCTTTTCCGATTGGTGTCATAGACAGACAAGATTTATTCTCTAATTGCATGCTGCCTACCATTTTATCACGGGACCAAGAACCTTGTGTTCGATCGATTTCTTGGTCCCCCGTTGGGCTTGCTCCGAACGCTGGCTGCTTGCTGGCTGTCTGCACCACCCAAGGATTCGTCAAGCTTTATCGCTCTCCATATTGCGATTTTTGTGCTGAATGGATAGAGGTTGCAAATGTATCGGCCAAGCTTTATGATTATCTTCAGAGTATAAATTTTGGGGAGGCATGGGCTTCTTCATCAAAACAGCAAGATGTAAATCATGAAATAGAGCCTGAGATTGACTATGACCCAGTGAAACAAAAGAGTTCAAATAAGATTGTTCGTGCTTCAAAATCGAAAGTAAAACCTGCCAAGGAGATACCAGAAAACAGCACACTCCCTCTTATAACTGCAGATCAATATGCTTCTCGCACGGCAATGCTATCAACTCTTGTAGTTGCCTGGTCGCCAATGCTGCATTCACTATCCAAAACCTGTTCAGTTCCTCAAGATGGTTTGTCCATGTCGTTACTTGCAGTTGGAGGGAAGTCCGGGAAAGTTTCTGTATGGAGAATTCCTATACCAGAATGCTACTCTGTTGATCAAAGCAGGGTCCCGGAAACTGTGGCACTCATAGGGATTCTTCAGGCGCACAATTCATGGATCACAGCTATTGGTTGGGCGTTGCTTGATTCTGATTCTTCAAATCCTAGAGTTTTGTTGGCTACAGCTAGTACTGATGGGAGTGTGAGGATCTGGCTGGGGAATAATGAAAAGTTGCTGAAGTCATCAGAACCTAGCGACACTTCCTTTTCCCTATTGCAGGAGGTTGCAAGCGATGCTGCCCCAGTTTTTGTACTTTCAGTCATTGTGCCTACTAAGTCTCCAGACAAAATTCACTTAGCAGCTGGAAAAGGATCTGGCTCTTTTGAATTGTGGATATGCAACATATCTAGCCAAAATTTTGACAAGATTGGCACATATGATGCACATGGCCAGACGGTTACAGGATTAGCTTGGGCGTTTGATGGGCAATCTTTGTATAGCTGCAGCCAGGATAATGTTGTACGCTGTTGGATTTTGAGTGGAAGTTCTCTCTGTGAAGTACCTATTCCTTCAAATACTCCTCGTTTGAGAAACTCAACTGATTTACCAGAAGAATTTGTTTCTTGCTTTGGTCTGGCAGTGTCCCCTGGAAATCTTGTGATTGCTTGGGTTCGAAATACTGATGTTGAACAATTAAACCCAATGTACGAGGCAAGGACTCAAAAGGCCATTGTTGAGTTTTTCTGGACCGGTGGGCAGCAAGTAGATGTCTCGCCAAACAATTCCCTACATTTTGATGTTGAAGCGATTCCTGGTTTTTCTGAGGAGTTGGTTTATTGGGAGTCCAACTTTCTATGGTCCTTAAAGCAGTATGAAATGCAAGATAAACCTCTGGTTGTTTGGGATATTGTAACAGCATTGTTGGCTTTCAATCATTCTAAACCAGAGTTTGTAGAAGAAGTACTGATTAAGTGGCTCTCTATTTCATACTTGGGATCTCAAACGGGCATTCCTGCTGAAACGGTTTTGTTGAATGTCTCCCGAAGTTGCTCAATAGTTACTTCTCGCCAATTACACCTTCTCAATATAATCTGCAGACGTGTGATTCTATCAGAGATGAAGGCTGATGAAATAAACAGTAAATTACTGAATTTGAAAGGAGTACATGGTGCTGAAAAAGAGAAGCGGTCTTTGTGGATCAATCTGCTTTTGAACAGTGAAAAAGAACTCCGGGAAAGGCTTGTGGGATTTACATTTTCTGCTCTTTTAAGTCAAATGCCTACTTCAGCCCCAAATTCTCCACCTGGAAAGTGGTTTCCTGTTGGCTTAGCACAAATGGAGCAGTGGATTGAGCTTAATCATGATCATGTGCACGGTCAGCTAAAGGTCCTCGCATCTGAAGTTGGAAAGCGGGATAGGAGGTTTAAGTCCAGTAAATGCACATCAGCAGAGACGTGCAGCTATTGTTTAGCATCAGTTCCATTTGAATCTCCAGAAGTTGCCATCTGCAGCGGAAAGGGCCACAAACTAGTCAGGTGTTCCATTTCTATGGAGATTTGTCCTACTACTCCGACATGGTTTTGCACATGCTGTCATAGACGAGCTTCCAAACCGGCACCAGCGATTCTGTTTGCAACCCCCGGTCTTCCTTTGAATTTCAAATCTTTGGCCGCATCGCCTTTGCTAGACACATCCTTAAAACCACTGTGTCCCTTTTGTGGTATACTGCTACAAAGACTACAACCGGATTTCCTACTTTCTGCATCACCAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TFIIIC_delta WD40 zf-TFIIIC

Protein Analysis

857

Amino Acids

93.99

Weight (kDa)

6.07

Isoelectric Point (pI)

49.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIIC_delta PF12657 36 - 490 6.1e-26 Transcription factor IIIC subunit delta bet-propeller domain
Beta-prop_WDR3_1st PF25173 375 - 442 6.8e-06 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 384 - 446 7.4e-06 CDC20/Fizzy WD40 domain
WD40 PF00400 407 - 442 1.6e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 656
Acc36I ACCTGC 1 cut(s) 625
AccB1I GGYRCC 1 cut(s) 2410
AccB7I CCANNNNNTGG 1 cut(s) 105
AccI GTMKAC 1 cut(s) 2518
AciI CCGC 4 cut(s) 1981, 2206, 2307, 2466
AclWI GGATC 5 cut(s) 920, 1010, 1174, 1798, 2000
AcoI YGGCCR 4 cut(s) 139, 450, 1243, 2463
AcsI RAATTY 8 cut(s) 481, 822, 1143, 1210, 1409, 1945, 2089, 2449
AcuI CTGAAG 5 cut(s) 455, 878, 1052, 2064, 2214
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 7 cut(s) 985, 1107, 1317, 1356, 1502, 1758, 1959
AfiI CCNNNNNNNGG 8 cut(s) 58, 105, 163, 330, 347, 761, 1072, 2513
AflIII ACRYGT 1 cut(s) 1894
AgeI ACCGGT 1 cut(s) 1544
AhlI ACTAGT 1 cut(s) 2323
AjiI CACGTC 2 cut(s) 1895, 2250
AjnI CCWGG 6 cut(s) 57, 710, 1302, 1441, 1606, 2098
AjuI GAANNNNNNNTTGG 4 cut(s) 712, 744, 1857, 1889
Alw21I GWGCWC 2 cut(s) 72, 2169
Alw26I GTCTC 4 cut(s) 1137, 1570, 1837, 2240
Alw44I GTGCAC 1 cut(s) 2165
AlwI GGATC 5 cut(s) 920, 1010, 1174, 1798, 2000
AoxI GGCC 8 cut(s) 60, 139, 190, 450, 1243, 1521, 2314, 2463
ApaLI GTGCAC 1 cut(s) 2165
ApoI RAATTY 8 cut(s) 481, 822, 1143, 1210, 1409, 1945, 2089, 2449
AsiGI ACCGGT 1 cut(s) 1544
Asp700I GAANNNNTTC 1 cut(s) 1602
AspLEI GCGC 2 cut(s) 80, 901
AspS9I GGNCC 9 cut(s) 108, 182, 289, 321, 862, 1542, 1653, 2182, 2314
AsuC2I CCSGG 4 cut(s) 802, 866, 2027, 2436
AsuHPI GGTGA 3 cut(s) 142, 190, 2553
AsuII TTCGAA 1 cut(s) 1468
AvaII GGWCC 8 cut(s) 108, 182, 289, 321, 862, 1542, 1653, 2182
BaeGI GKGCMC 1 cut(s) 2169
BalI TGGCCA 1 cut(s) 1245
BanI GGYRCC 1 cut(s) 2410
BbsI GAAGAC 1 cut(s) 2432
Bbv12I GWGCWC 2 cut(s) 72, 2169
BccI CCATC 7 cut(s) 91, 107, 161, 755, 983, 1271, 2306
BceAI ACGGC 2 cut(s) 113, 699
BcgI CGANNNNNNTGC 4 cut(s) 401, 429, 435, 463
BciT130I CCWGG 6 cut(s) 59, 712, 1304, 1443, 1608, 2100
BclI TGATCA 2 cut(s) 850, 2158
BcnI CCSGG 4 cut(s) 802, 866, 2027, 2436
BcoDI GTCTC 4 cut(s) 1137, 1570, 1837, 2240
BcuI ACTAGT 1 cut(s) 2323
BfaI CTAG 6 cut(s) 960, 981, 1047, 1203, 2324, 2480
BfmI CTRYAG 5 cut(s) 660, 975, 1297, 1888, 2302
BfoI RGCGCY 1 cut(s) 81
BfuAI ACCTGC 1 cut(s) 625
BlpI GCTNAGC 1 cut(s) 2122
BmcAI AGTACT 2 cut(s) 985, 1758
Bme18I GGWCC 8 cut(s) 108, 182, 289, 321, 862, 1542, 1653, 2182
BmgBI CACGTC 2 cut(s) 1895, 2250
BmgT120I GGNCC 9 cut(s) 108, 182, 289, 321, 862, 1542, 1653, 2182, 2314
BmiI GGNNCC 5 cut(s) 290, 323, 863, 864, 2412
BmrI ACTGGG 2 cut(s) 554, 1091
BmsI GCATC 6 cut(s) 1078, 1225, 2198, 2276, 2477, 2567
BmuI ACTGGG 2 cut(s) 554, 1091
BpiI GAAGAC 1 cut(s) 2432
BpmI CTGGAG 2 cut(s) 1119, 2271
Bpu1102I GCTNAGC 1 cut(s) 2122
Bpu14I TTCGAA 1 cut(s) 1468
BpuEI CTTGAG 1 cut(s) 741
BpuMI CCSGG 4 cut(s) 802, 866, 2027, 2436
Bsa29I ATCGAT 1 cut(s) 311
BsaBI GATNNNNATC 1 cut(s) 525
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 4 cut(s) 376, 621, 1441, 2434
BsaWI WCCGGW 2 cut(s) 1544, 2539
BsaXI ACNNNNNCTCC 4 cut(s) 32, 62, 1117, 1147
Bsc4I CCNNNNNNNGG 8 cut(s) 58, 105, 163, 330, 347, 761, 1072, 2513
Bse118I RCCGGY 2 cut(s) 1544, 2407
Bse1I ACTGG 3 cut(s) 560, 1097, 2226
Bse3DI GCAATG 1 cut(s) 693
Bse8I GATNNNNATC 1 cut(s) 525
BseBI CCWGG 6 cut(s) 59, 712, 1304, 1443, 1608, 2100
BseCI ATCGAT 1 cut(s) 311
BseDI CCNNGG 4 cut(s) 376, 621, 1441, 2434
BseGI GGATG 3 cut(s) 83, 153, 2486
BseJI GATNNNNATC 1 cut(s) 525
BseLI CCNNNNNNNGG 8 cut(s) 58, 105, 163, 330, 347, 761, 1072, 2513
BseMI GCAATG 1 cut(s) 693
BseMII CTCAG 2 cut(s) 534, 1608
BseNI ACTGG 3 cut(s) 560, 1097, 2226
BseRI GAGGAG 2 cut(s) 1365, 1634
BseSI GKGCMC 1 cut(s) 2169
BseYI CCCAGC 1 cut(s) 1009
BsgI GTGCAG 2 cut(s) 352, 2272
Bsh1285I CGRYCG 1 cut(s) 311
BshFI GGCC 8 cut(s) 62, 141, 192, 452, 1245, 1523, 2316, 2465
BshNI GGYRCC 1 cut(s) 2410
BshTI ACCGGT 1 cut(s) 1544
BshVI ATCGAT 1 cut(s) 311
BsiEI CGRYCG 1 cut(s) 311
BsiHKAI GWGCWC 2 cut(s) 72, 2169
BsiSI CCGG 9 cut(s) 138, 164, 801, 866, 1545, 2026, 2408, 2436, 2540
BslFI GGGAC 5 cut(s) 302, 307, 848, 1423, 2490
BslI CCNNNNNNNGG 8 cut(s) 58, 105, 163, 330, 347, 761, 1072, 2513
BsmAI GTCTC 4 cut(s) 1137, 1570, 1837, 2240
BsmBI CGTCTC 1 cut(s) 2240
BsmFI GGGAC 5 cut(s) 302, 307, 848, 1423, 2490
BsmI GAATGC 3 cut(s) 727, 842, 1803
BsnI GGCC 8 cut(s) 62, 141, 192, 452, 1245, 1523, 2316, 2465
Bsp119I TTCGAA 1 cut(s) 1468
Bsp1286I GDGCHC 2 cut(s) 72, 2169
Bsp143I GATC 9 cut(s) 308, 664, 850, 912, 1002, 1166, 1790, 1992, 2158
Bsp1720I GCTNAGC 1 cut(s) 2122
BspACI CCGC 4 cut(s) 1981, 2206, 2307, 2466
BspANI GGCC 8 cut(s) 62, 141, 192, 452, 1245, 1523, 2316, 2465
BspCNI CTCAG 2 cut(s) 535, 1609
BspDI ATCGAT 1 cut(s) 311
BspHI TCATGA 2 cut(s) 529, 2155
BspLI GGNNCC 5 cut(s) 290, 323, 863, 864, 2412
BspMAI CTGCAG 4 cut(s) 664, 1301, 1892, 2306
BspMI ACCTGC 1 cut(s) 625
BspPI GGATC 5 cut(s) 920, 1010, 1174, 1798, 2000
BspT104I TTCGAA 1 cut(s) 1468
BspT107I GGYRCC 1 cut(s) 2410
BsrDI GCAATG 1 cut(s) 693
BsrFI RCCGGY 2 cut(s) 1544, 2407
BsrI ACTGG 3 cut(s) 560, 1097, 2226
BssAI RCCGGY 2 cut(s) 1544, 2407
BssECI CCNNGG 4 cut(s) 376, 621, 1441, 2434
BssMI GATC 9 cut(s) 308, 664, 850, 912, 1002, 1166, 1790, 1992, 2158
BssNAI GTATAC 1 cut(s) 2519
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 2 cut(s) 376, 621
Bst1107I GTATAC 1 cut(s) 2519
Bst2UI CCWGG 6 cut(s) 59, 712, 1304, 1443, 1608, 2100
Bst4CI ACNGT 8 cut(s) 67, 874, 1252, 1819, 1934, 2012, 2171, 2502
BstACI GRCGYC 1 cut(s) 96
BstAPI GCANNNNNTGC 1 cut(s) 1088
BstBI TTCGAA 1 cut(s) 1468
BstC8I GCNNGC 6 cut(s) 266, 336, 349, 356, 360, 1084
BstDEI CTNAG 5 cut(s) 543, 1128, 1150, 1617, 2122
BstENI CCTNNNNNAGG 1 cut(s) 1070
BstF5I GGATG 3 cut(s) 83, 153, 2486
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 2 cut(s) 80, 901
BstKTI GATC 9 cut(s) 311, 667, 853, 915, 1005, 1169, 1793, 1995, 2161
BstMAI GTCTC 4 cut(s) 1137, 1570, 1837, 2240
BstMBI GATC 9 cut(s) 308, 664, 850, 912, 1002, 1166, 1790, 1992, 2158
BstMCI CGRYCG 1 cut(s) 311
BstMWI GCNNNNNNNGC 6 cut(s) 344, 724, 1088, 1718, 2173, 2313
BstNI CCWGG 6 cut(s) 59, 712, 1304, 1443, 1608, 2100
BstNSI RCATGY 2 cut(s) 268, 2385
BstSFI CTRYAG 5 cut(s) 660, 975, 1297, 1888, 2302
BstSLI GKGCMC 1 cut(s) 2169
BstV2I GAAGAC 1 cut(s) 2432
BstX2I RGATCY 3 cut(s) 1002, 1166, 1790
BstXI CCANNNNNNTGG 1 cut(s) 2344
BstYI RGATCY 3 cut(s) 1002, 1166, 1790
BstZ17I GTATAC 1 cut(s) 2519
Bsu15I ATCGAT 1 cut(s) 311
BsuRI GGCC 8 cut(s) 62, 141, 192, 452, 1245, 1523, 2316, 2465
BsuTUI ATCGAT 1 cut(s) 311
BtgZI GCGATG 4 cut(s) 85, 109, 1101, 2454
BtrI CACGTC 2 cut(s) 1895, 2250
BtsCI GGATG 3 cut(s) 83, 153, 2486
BtsI GCAGTG 2 cut(s) 1440, 2144
BtsIMutI CAGTG 5 cut(s) 567, 1440, 2017, 2144, 2498
BveI ACCTGC 1 cut(s) 625
Cac8I GCNNGC 6 cut(s) 266, 336, 349, 356, 360, 1084
CciI TCATGA 2 cut(s) 529, 2155
CfoI GCGC 2 cut(s) 80, 901
Cfr10I RCCGGY 2 cut(s) 1544, 2407
Cfr13I GGNCC 9 cut(s) 108, 182, 289, 321, 862, 1542, 1653, 2182, 2314
ClaI ATCGAT 1 cut(s) 311
CseI GACGC 1 cut(s) 104
Csp6I GTAC 7 cut(s) 984, 1106, 1316, 1355, 1501, 1757, 1958
CspAI ACCGGT 1 cut(s) 1544
CviQI GTAC 7 cut(s) 984, 1106, 1316, 1355, 1501, 1757, 1958
DdeI CTNAG 5 cut(s) 543, 1128, 1150, 1617, 2122
DpnI GATC 9 cut(s) 310, 666, 852, 914, 1004, 1168, 1792, 1994, 2160
DpnII GATC 9 cut(s) 308, 664, 850, 912, 1002, 1166, 1790, 1992, 2158
EaeI YGGCCR 4 cut(s) 139, 450, 1243, 2463
Eco130I CCWWGG 2 cut(s) 376, 621
Eco147I AGGCCT 1 cut(s) 192
Eco47I GGWCC 8 cut(s) 108, 182, 289, 321, 862, 1542, 1653, 2182
Eco57I CTGAAG 5 cut(s) 455, 878, 1052, 2064, 2214
EcoNI CCTNNNNNAGG 1 cut(s) 1070
EcoO109I RGGNCCY 2 cut(s) 862, 2182
EcoRI GAATTC 1 cut(s) 822
EcoRII CCWGG 6 cut(s) 57, 710, 1302, 1441, 1606, 2098
EcoT14I CCWWGG 2 cut(s) 376, 621
ErhI CCWWGG 2 cut(s) 376, 621
Esp3I CGTCTC 1 cut(s) 2240
FaqI GGGAC 5 cut(s) 302, 307, 848, 1423, 2490
FauI CCCGC 1 cut(s) 2199
FauNDI CATATG 1 cut(s) 1231
FbaI TGATCA 2 cut(s) 850, 2158
FblI GTMKAC 1 cut(s) 2518
FokI GGATG 3 cut(s) 70, 140, 2473
FspBI CTAG 6 cut(s) 960, 981, 1047, 1203, 2324, 2480
GlaI GCGC 2 cut(s) 79, 900
GsaI CCCAGC 1 cut(s) 1013
GsuI CTGGAG 2 cut(s) 1119, 2271
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 8 cut(s) 62, 141, 192, 452, 1245, 1523, 2316, 2465
HapII CCGG 9 cut(s) 138, 164, 801, 866, 1545, 2026, 2408, 2436, 2540
HgaI GACGC 1 cut(s) 104
HhaI GCGC 2 cut(s) 80, 901
Hin1I GRCGYC 1 cut(s) 96
Hin6I GCGC 2 cut(s) 78, 899
HinP1I GCGC 2 cut(s) 78, 899
HindIII AAGCTT 2 cut(s) 389, 455
HpaII CCGG 9 cut(s) 138, 164, 801, 866, 1545, 2026, 2408, 2436, 2540
HphI GGTGA 3 cut(s) 142, 190, 2553
Hpy166II GTNNAC 2 cut(s) 2167, 2519
Hpy188III TCNNGA 8 cut(s) 157, 530, 758, 1136, 1540, 1836, 2156, 2288
Hpy8I GTNNAC 2 cut(s) 2167, 2519
Hpy99I CGWCG 4 cut(s) 77, 98, 116, 134
HpyAV CCTTC 4 cut(s) 97, 1374, 1883, 1909
HpyCH4III ACNGT 8 cut(s) 67, 874, 1252, 1819, 1934, 2012, 2171, 2502
HpyCH4IV ACGT 2 cut(s) 1894, 2249
HpyF10VI GCNNNNNNNGC 6 cut(s) 344, 724, 1088, 1718, 2173, 2313
HpyF3I CTNAG 5 cut(s) 543, 1128, 1150, 1617, 2122
HpySE526I ACGT 2 cut(s) 1894, 2249
Hsp92I GRCGYC 1 cut(s) 96
HspAI GCGC 2 cut(s) 78, 899
KflI GGGWCCC 1 cut(s) 862
Ksp22I TGATCA 2 cut(s) 850, 2158
Kzo9I GATC 9 cut(s) 308, 664, 850, 912, 1002, 1166, 1790, 1992, 2158
LmnI GCTCC 3 cut(s) 85, 343, 2134
LweI GCATC 6 cut(s) 1078, 1225, 2198, 2276, 2477, 2567
MaeI CTAG 6 cut(s) 960, 981, 1047, 1203, 2324, 2480
MaeII ACGT 2 cut(s) 1894, 2249
MaeIII GTNAC 5 cut(s) 148, 777, 1252, 1705, 1852
MalI GATC 9 cut(s) 310, 666, 852, 914, 1004, 1168, 1792, 1994, 2160
MboI GATC 9 cut(s) 308, 664, 850, 912, 1002, 1166, 1790, 1992, 2158
MboII GAAGA 7 cut(s) 461, 495, 884, 942, 1418, 1763, 2432
MflI RGATCY 3 cut(s) 1002, 1166, 1790
MhlI GDGCHC 2 cut(s) 72, 2169
MlsI TGGCCA 1 cut(s) 1245
MluNI TGGCCA 1 cut(s) 1245
MlyI GAGTC 3 cut(s) 41, 1507, 1646
MmeI TCCRAC 6 cut(s) 135, 769, 1304, 1665, 2179, 2390
Mox20I TGGCCA 1 cut(s) 1245
MroXI GAANNNNTTC 1 cut(s) 1602
MscI TGGCCA 1 cut(s) 1245
MseI TTAA 8 cut(s) 1490, 1658, 1764, 2063, 2151, 2220, 2492, 2569
MslI CAYNNNNRTG 4 cut(s) 686, 1590, 2160, 2342
Msp20I TGGCCA 1 cut(s) 1245
MspA1I CMGCKG 2 cut(s) 1157, 2307
MspI CCGG 9 cut(s) 138, 164, 801, 866, 1545, 2026, 2408, 2436, 2540
Mva1269I GAATGC 3 cut(s) 727, 842, 1803
MvaI CCWGG 6 cut(s) 59, 712, 1304, 1443, 1608, 2100
MwoI GCNNNNNNNGC 6 cut(s) 344, 724, 1088, 1718, 2173, 2313
NciI CCSGG 4 cut(s) 802, 866, 2027, 2436
NdeI CATATG 1 cut(s) 1231
NdeII GATC 9 cut(s) 308, 664, 850, 912, 1002, 1166, 1790, 1992, 2158
NlaIV GGNNCC 5 cut(s) 290, 323, 863, 864, 2412
NmuCI GTSAC 1 cut(s) 148
NspI RCATGY 2 cut(s) 268, 2385
NspV TTCGAA 1 cut(s) 1468
PaeI GCATGC 1 cut(s) 268
PagI TCATGA 2 cut(s) 529, 2155
PceI AGGCCT 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PctI GAATGC 3 cut(s) 727, 842, 1803
PdmI GAANNNNTTC 1 cut(s) 1602
PfeI GAWTC 9 cut(s) 160, 381, 889, 941, 947, 1603, 1900, 2282, 2419
PflMI CCANNNNNTGG 1 cut(s) 105
PfoI TCCNGGA 3 cut(s) 800, 864, 2025
PinAI ACCGGT 1 cut(s) 1544
Ple19I CGATCG 1 cut(s) 311
PleI GAGTC 3 cut(s) 41, 1507, 1645
PpsI GAGTC 3 cut(s) 41, 1507, 1645
PpuMI RGGWCCY 2 cut(s) 862, 2182
PsiI TTATAA 1 cut(s) 656
Psp5II RGGWCCY 2 cut(s) 862, 2182
Psp6I CCWGG 6 cut(s) 57, 710, 1302, 1441, 1606, 2098
PspFI CCCAGC 1 cut(s) 1009
PspGI CCWGG 6 cut(s) 57, 710, 1302, 1441, 1606, 2098
PspN4I GGNNCC 5 cut(s) 290, 323, 863, 864, 2412
PspPI GGNCC 9 cut(s) 108, 182, 289, 321, 862, 1542, 1653, 2182, 2314
PspPPI RGGWCCY 2 cut(s) 862, 2182
PstI CTGCAG 4 cut(s) 664, 1301, 1892, 2306
PsuI RGATCY 3 cut(s) 1002, 1166, 1790
PvuI CGATCG 1 cut(s) 311
PvuII CAGCTG 1 cut(s) 1157
RsaI GTAC 7 cut(s) 985, 1107, 1317, 1356, 1502, 1758, 1959
RsaNI GTAC 7 cut(s) 984, 1106, 1316, 1355, 1501, 1757, 1958
RseI CAYNNNNRTG 4 cut(s) 686, 1590, 2160, 2342
SaqAI TTAA 8 cut(s) 1490, 1658, 1764, 2063, 2151, 2220, 2492, 2569
Sau3AI GATC 9 cut(s) 308, 664, 850, 912, 1002, 1166, 1790, 1992, 2158
Sau96I GGNCC 9 cut(s) 108, 182, 289, 321, 862, 1542, 1653, 2182, 2314
ScaI AGTACT 2 cut(s) 985, 1758
SchI GAGTC 3 cut(s) 41, 1507, 1646
SduI GDGCHC 2 cut(s) 72, 2169
SfaNI GCATC 6 cut(s) 1078, 1225, 2198, 2276, 2477, 2567
SfcI CTRYAG 5 cut(s) 660, 975, 1297, 1888, 2302
SfuI TTCGAA 1 cut(s) 1468
SinI GGWCC 8 cut(s) 108, 182, 289, 321, 862, 1542, 1653, 2182
SmiMI CAYNNNNRTG 4 cut(s) 686, 1590, 2160, 2342
SmlI CTYRAG 1 cut(s) 756
SmoI CTYRAG 1 cut(s) 756
SpeI ACTAGT 1 cut(s) 2323
SphI GCATGC 1 cut(s) 268
SseBI AGGCCT 1 cut(s) 192
SsiI CCGC 4 cut(s) 1981, 2206, 2307, 2466
SspMI CTAG 6 cut(s) 960, 981, 1047, 1203, 2324, 2480
StuI AGGCCT 1 cut(s) 192
StyI CCWWGG 2 cut(s) 376, 621
TaaI ACNGT 8 cut(s) 67, 874, 1252, 1819, 1934, 2012, 2171, 2502
TaiI ACGT 2 cut(s) 1897, 2252
TaqI TCGA 4 cut(s) 307, 311, 605, 1468
TatI WGTACW 4 cut(s) 983, 1105, 1756, 1957
TauI GCSGC 1 cut(s) 2468
TfiI GAWTC 9 cut(s) 160, 381, 889, 941, 947, 1603, 1900, 2282, 2419
Tru1I TTAA 8 cut(s) 1490, 1658, 1764, 2063, 2151, 2220, 2492, 2569
Tru9I TTAA 8 cut(s) 1490, 1658, 1764, 2063, 2151, 2220, 2492, 2569
TscAI CASTG 5 cut(s) 567, 1440, 2017, 2144, 2505
TseFI GTSAC 1 cut(s) 148
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 8 cut(s) 495, 546, 897, 1035, 1683, 1770, 1928, 1938
TspRI CASTG 5 cut(s) 567, 1440, 2017, 2144, 2505
Van91I CCANNNNNTGG 1 cut(s) 105
VneI GTGCAC 1 cut(s) 2165
VpaK11BI GGWCC 8 cut(s) 108, 182, 289, 321, 862, 1542, 1653, 2182
XagI CCTNNNNNAGG 1 cut(s) 1070
XapI RAATTY 8 cut(s) 481, 822, 1143, 1210, 1409, 1945, 2089, 2449
XceI RCATGY 2 cut(s) 268, 2385
XcmI CCANNNNNNNNNTGG 1 cut(s) 2103
XmiI GTMKAC 1 cut(s) 2518
XmnI GAANNNNTTC 1 cut(s) 1602
XspI CTAG 6 cut(s) 960, 981, 1047, 1203, 2324, 2480
ZrmI AGTACT 2 cut(s) 985, 1758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.