Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0017204

Physical Location & Seq

Genomic Coordinates Reverse (-)

4710689 .. 4711480

Gene Structure 792 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1040300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 792 bp

ATGGCAGCCAAGTCTTTCCAATCCATACGCCACAATCACCACTCACTCCTCCTCCACCACCACCATATAGACAATGCTCCCTCCACCTTCTTCCTTAAACCCACCACCACAAACACCCTAACTTCTCCTCTCTCTCATCACTCTCTCTTCTCCACCAAAACTTTCTCAATCTCCGCCACCACATCATTCTCCACACCATCTTCCTCCACCCAATCCACCTCCTTCGACCTCCTAGAACGACACCTCTCCGCCCAAAACTTCCGCGAAGCCGACGAGGAGACCCGCCGCCTCCTCATCGTCCTCGCCGGAGAACAAGCCCAAAAGCGAGGCTACGTCTTCTTCTCCGAGGTCCAGTTCATCTCCGAAGCCGACCTCCAAACCATCGACGCCCTATGGAGACAGCACAGCAACAACAAATTCGGGTACAGCGTCCAGAAAAAAATTTACGACAAGGTAGGCGACGACTTCACCAAGTTCTTCATCAAGTTAGGGTGGATGAAGAAGCTTGACACAGAAGTTTTGCAATACAACTATAGAGCTTTTCCCGGTGAGTTCATCTGGGAGCTCAATGAGAGCACTCCAGAGGGTCATCTGCCATTGACAAATGCTTTGAGAGGTACCCAGTTGCTTAAAAGCATTTTAAGCCATCCAGCTTTCAAGCCCACTGAAGAAGAAGTGCTTCTAGCAAAAGCTGCTGCTGAAGCCGAAGGTAACATTGGAGTTACAGGTTTGAAGGGTTTCATGGATGGCTCCAAAAGCAAAAGAGTGCTCAAATCAGATTATAGCTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009507 GO:0009536 GO:0009987 GO:0010019 GO:0015994 GO:0015995 GO:0018130 GO:0019438 GO:0019899 GO:0023052 GO:0033013 GO:0033014 GO:0034641 GO:0042440 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044093 GO:0044237 GO:0044249 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046483 GO:0046906 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051186 GO:0051188 GO:0051716 GO:0065007 GO:0065009 GO:0071704 GO:0097159 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GUN4

Protein Analysis

264

Amino Acids

29.75

Weight (kDa)

7.97

Isoelectric Point (pI)

36.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUN4	PF05419	69 - 217	6.5e-51	GUN4-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0017204)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59400
fragaria_vesca	FvH4_4g10790
malus_domestica	MD04G1040300.v1.1
prunus_persica	Prupe.1G193000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0406801
rosa_laevigata	RLG00000008758
rosa_multiflora	Rmu_sc0006408.1_g000008
rosa_roxburghii	Rroxscaffold_5G00351580
rosa_rugosa	Rorug04G0073400
rosa_samantha	Rh4AG140700 Rh4CG147600 Rh4DG134700
rosa_wichuraiana	Rw4G011450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	617
AccB1I	GGYRCC	1 cut(s)	617
AccII	CGCG	1 cut(s)	264
AciI	CCGC	5 cut(s)	174, 249, 262, 283, 286
AcsI	RAATTY	2 cut(s)	416, 441
AcuI	CTGAAG	2 cut(s)	687, 720
AcyI	GRCGYC	1 cut(s)	387
AfaI	GTAC	2 cut(s)	425, 619
AgsI	TTSAA	2 cut(s)	658, 733
AjuI	GAANNNNNNNTTGG	2 cut(s)	699, 731
AluBI	AGCT	6 cut(s)	505, 539, 565, 653, 692, 786
AluI	AGCT	6 cut(s)	505, 539, 565, 653, 692, 786
Alw21I	GWGCWC	3 cut(s)	567, 578, 771
Alw26I	GTCTC	2 cut(s)	272, 391
ApeKI	GCWGC	3 cut(s)	5, 692, 695
ApoI	RAATTY	2 cut(s)	416, 441
ArsI	GACNNNNNNTTYG	2 cut(s)	592, 624
Asp700I	GAANNNNTTC	2 cut(s)	678, 737
Asp718I	GGTACC	1 cut(s)	617
AspS9I	GGNCC	1 cut(s)	349
AsuC2I	CCSGG	1 cut(s)	546
AsuHPI	GGTGA	3 cut(s)	29, 460, 560
AvaII	GGWCC	1 cut(s)	349
BanI	GGYRCC	1 cut(s)	617
BanII	GRGCYC	1 cut(s)	567
BbsI	GAAGAC	1 cut(s)	328
Bbv12I	GWGCWC	3 cut(s)	567, 578, 771
BbvI	GCAGC	3 cut(s)	17, 679, 682
BccI	CCATC	4 cut(s)	205, 389, 654, 740
BcnI	CCSGG	1 cut(s)	546
BcoDI	GTCTC	2 cut(s)	272, 391
BfaI	CTAG	2 cut(s)	233, 683
BfmI	CTRYAG	1 cut(s)	532
BisI	GCNGC	4 cut(s)	6, 286, 693, 696
BlsI	GCNGC	4 cut(s)	7, 287, 694, 697
Bme1390I	CCNGG	1 cut(s)	546
Bme18I	GGWCC	1 cut(s)	349
BmgT120I	GGNCC	1 cut(s)	349
BmiI	GGNNCC	2 cut(s)	619, 751
BmrFI	CCNGG	1 cut(s)	546
BmrI	ACTGGG	1 cut(s)	616
BmuI	ACTGGG	1 cut(s)	616
BpiI	GAAGAC	1 cut(s)	328
BpmI	CTGGAG	1 cut(s)	564
BpuMI	CCSGG	1 cut(s)	546
BsaHI	GRCGYC	1 cut(s)	387
BsaI	GGTCTC	1 cut(s)	272
BsaJI	CCNNGG	1 cut(s)	345
BsaXI	ACNNNNNCTCC	2 cut(s)	36, 66
Bse1I	ACTGG	2 cut(s)	352, 622
BseDI	CCNNGG	1 cut(s)	345
BseGI	GGATG	3 cut(s)	501, 646, 751
BseNI	ACTGG	2 cut(s)	352, 622
BseRI	GAGGAG	5 cut(s)	38, 41, 117, 281, 290
BseXI	GCAGC	3 cut(s)	17, 679, 682
Bsh1236I	CGCG	1 cut(s)	264
BshNI	GGYRCC	1 cut(s)	617
BsiHKAI	GWGCWC	3 cut(s)	567, 578, 771
BsiSI	CCGG	2 cut(s)	306, 546
BsmAI	GTCTC	2 cut(s)	272, 391
Bso31I	GGTCTC	1 cut(s)	272
Bsp1286I	GDGCHC	3 cut(s)	567, 578, 771
BspACI	CCGC	5 cut(s)	174, 249, 262, 283, 286
BspFNI	CGCG	1 cut(s)	264
BspLI	GGNNCC	2 cut(s)	619, 751
BspT107I	GGYRCC	1 cut(s)	617
BspTNI	GGTCTC	1 cut(s)	272
BsrI	ACTGG	2 cut(s)	352, 622
BssECI	CCNNGG	1 cut(s)	345
BssNI	GRCGYC	1 cut(s)	387
Bst6I	CTCTTC	1 cut(s)	152
BstACI	GRCGYC	1 cut(s)	387
BstAPI	GCANNNNNTGC	1 cut(s)	692
BstF5I	GGATG	3 cut(s)	501, 646, 751
BstFNI	CGCG	1 cut(s)	264
BstMAI	GTCTC	2 cut(s)	272, 391
BstMWI	GCNNNNNNNGC	4 cut(s)	642, 692, 701, 756
BstSCI	CCNGG	1 cut(s)	544
BstSFI	CTRYAG	1 cut(s)	532
BstUI	CGCG	1 cut(s)	264
BstV1I	GCAGC	3 cut(s)	17, 679, 682
BstV2I	GAAGAC	1 cut(s)	328
BtsCI	GGATG	3 cut(s)	501, 646, 751
BtsIMutI	CAGTG	1 cut(s)	663
Cfr13I	GGNCC	1 cut(s)	349
CseI	GACGC	2 cut(s)	395, 418
Csp6I	GTAC	2 cut(s)	424, 618
CviAII	CATG	1 cut(s)	742
CviQI	GTAC	2 cut(s)	424, 618
Eam1104I	CTCTTC	1 cut(s)	152
EarI	CTCTTC	1 cut(s)	152
EciI	GGCGGA	2 cut(s)	163, 238
Ecl136II	GAGCTC	1 cut(s)	565
Eco24I	GRGCYC	1 cut(s)	567
Eco31I	GGTCTC	1 cut(s)	272
Eco47I	GGWCC	1 cut(s)	349
Eco53kI	GAGCTC	1 cut(s)	565
Eco57I	CTGAAG	2 cut(s)	687, 720
EcoICRI	GAGCTC	1 cut(s)	565
EcoT38I	GRGCYC	1 cut(s)	567
FaeI	CATG	1 cut(s)	745
FaiI	YATR	7 cut(s)	26, 66, 68, 394, 534, 743, 783
FalI	AAGNNNNNCTT	2 cut(s)	663, 695
FatI	CATG	1 cut(s)	741
FauI	CCCGC	1 cut(s)	290
Fnu4HI	GCNGC	4 cut(s)	6, 286, 693, 696
FokI	GGATG	3 cut(s)	508, 633, 758
FriOI	GRGCYC	1 cut(s)	567
Fsp4HI	GCNGC	4 cut(s)	6, 286, 693, 696
FspBI	CTAG	2 cut(s)	233, 683
GluI	GCNGC	4 cut(s)	6, 286, 693, 696
GsuI	CTGGAG	1 cut(s)	564
HapII	CCGG	2 cut(s)	306, 546
HgaI	GACGC	2 cut(s)	395, 418
Hin1I	GRCGYC	1 cut(s)	387
Hin1II	CATG	1 cut(s)	745
HindIII	AAGCTT	1 cut(s)	503
HpaII	CCGG	2 cut(s)	306, 546
HphI	GGTGA	3 cut(s)	29, 460, 560
Hpy188I	TCNGA	3 cut(s)	346, 364, 778
Hpy188III	TCNNGA	2 cut(s)	433, 581
Hpy99I	CGWCG	3 cut(s)	275, 389, 464
HpyAV	CCTTC	4 cut(s)	97, 232, 701, 727
HpyCH4IV	ACGT	1 cut(s)	333
HpyCH4V	TGCA	1 cut(s)	523
HpyF10VI	GCNNNNNNNGC	4 cut(s)	642, 692, 701, 756
HpySE526I	ACGT	1 cut(s)	333
Hsp92I	GRCGYC	1 cut(s)	387
Hsp92II	CATG	1 cut(s)	745
KpnI	GGTACC	1 cut(s)	621
LmnI	GCTCC	3 cut(s)	82, 562, 755
LpnPI	CCDG	9 cut(s)	319, 365, 446, 544, 559, 594, 635, 663, 711
Lsp1109I	GCAGC	3 cut(s)	17, 679, 682
MaeI	CTAG	2 cut(s)	233, 683
MaeII	ACGT	1 cut(s)	333
MaeIII	GTNAC	2 cut(s)	710, 721
MboII	GAAGA	9 cut(s)	82, 139, 192, 328, 331, 469, 511, 680, 683
MhlI	GDGCHC	3 cut(s)	567, 578, 771
MluCI	AATT	2 cut(s)	416, 441
MroXI	GAANNNNTTC	2 cut(s)	678, 737
MseI	TTAA	4 cut(s)	96, 630, 641, 790
MspI	CCGG	2 cut(s)	306, 546
MspR9I	CCNGG	1 cut(s)	546
MvnI	CGCG	1 cut(s)	264
MwoI	GCNNNNNNNGC	4 cut(s)	642, 692, 701, 756
NciI	CCSGG	1 cut(s)	546
NlaIII	CATG	1 cut(s)	745
NlaIV	GGNNCC	2 cut(s)	619, 751
PcsI	WCGNNNNNNNCGW	1 cut(s)	426
PdmI	GAANNNNTTC	2 cut(s)	678, 737
PkrI	GCNGC	4 cut(s)	7, 287, 694, 697
Psp124BI	GAGCTC	1 cut(s)	567
PspN4I	GGNNCC	2 cut(s)	619, 751
PspPI	GGNCC	1 cut(s)	349
RsaI	GTAC	2 cut(s)	425, 619
RsaNI	GTAC	2 cut(s)	424, 618
SacI	GAGCTC	1 cut(s)	567
SaqAI	TTAA	4 cut(s)	96, 630, 641, 790
SatI	GCNGC	4 cut(s)	6, 286, 693, 696
Sau96I	GGNCC	1 cut(s)	349
ScrFI	CCNGG	1 cut(s)	546
SduI	GDGCHC	3 cut(s)	567, 578, 771
SfcI	CTRYAG	1 cut(s)	532
SinI	GGWCC	1 cut(s)	349
Sse9I	AATT	2 cut(s)	416, 441
SsiI	CCGC	5 cut(s)	174, 249, 262, 283, 286
SspMI	CTAG	2 cut(s)	233, 683
SstI	GAGCTC	1 cut(s)	567
StyD4I	CCNGG	1 cut(s)	544
TaiI	ACGT	1 cut(s)	336
TaqI	TCGA	2 cut(s)	225, 384
TasI	AATT	2 cut(s)	416, 441
TauI	GCSGC	1 cut(s)	288
Tru1I	TTAA	4 cut(s)	96, 630, 641, 790
Tru9I	TTAA	4 cut(s)	96, 630, 641, 790
TscAI	CASTG	1 cut(s)	670
TseI	GCWGC	3 cut(s)	5, 692, 695
TspDTI	ATGAA	5 cut(s)	346, 469, 512, 544, 730
TspRI	CASTG	1 cut(s)	670
VpaK11BI	GGWCC	1 cut(s)	349
XapI	RAATTY	2 cut(s)	416, 441
XmnI	GAANNNNTTC	2 cut(s)	678, 737
XspI	CTAG	2 cut(s)	233, 683

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.