Rh4DG134700

Tetrapyrrole-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0017204
Physical Location & Seq
Forward (+)
26805172 .. 26806930
1759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG134700.1

Sequence Viewer

Length: 831 bp
ATGGCCGTCAACACTTTCCAATCCACATACCACAATCACCACTCTACTACTCATGACATTCCTCCCTCCACCACCACTCTTTTCCTTAAACCCACACACACCACCGTAACCTCCTCCCCTCTCTCTTCTCATCACCACCTTCACTCTCTCGTGATCTCTTCAACCAAAACGTTCACAGTCTCGCCCACCACCTCATTTTCGACTCCTTCTTCTTCCCCCTCCTCCTCCAAATCCAACACCTTCGACCTCCTACAGCAGCACCTCTCCACCCAAAACTTCCGCGAAGCCGACGAAGAGACTCGCCGCCTCCTCATCGTCCTCGCAGGTGAGGCAGCCCAAAAGCGAGGATACGTCTTTTTCTCCGAGGTCCAGTTCATCCCCGAAGCCGACCTCAAAGCCATTGACGACCTCTGGAGACAGCACAGCAACAACAGGTTCGGCTACTCCGTCCAGAAGAGGATATTCTTCGAAAAGGTTAACCAGGACTTCACCAACTTCTTCATCAAAGTCGGGTGGATGAAGAAGCTCGACACCGAGGTCGACCAGTACAACTACAGAGCTTTCCCTAACGAATTCATCTGGGAGCTCAACGACGACACACCCGAGGGTCATCTGCCATTGACGAATGCTTTGAGAGGGACCCAGTTGCTGAAATGCGTTCTGAACCATCCAGCATTTAAGAACACCGAAGAAGAAGAAGAACGGCTACTCCAAGCGGGCAGGGCCGCCGTTGAAGGTAATAAAGCAGCTAGTGGTGGAGGGTTGAAGGGGTTAATGGATGGATCCAAACCTCTGAACAAGAGGGTGCTCGAATCAGACTATAGCTTTTGA

Protein Analysis

276

Amino Acids

31.23

Weight (kDa)

6.5

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUN4 PF05419 75 - 224 6.6e-50 GUN4-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59400
fragaria_vesca FvH4_4g10790
malus_domestica MD04G1040300.v1.1
prunus_persica Prupe.1G193000_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0406801
rosa_laevigata RLG00000008758
rosa_multiflora Rmu_sc0006408.1_g000008
rosa_roxburghii Rroxscaffold_5G00351580
rosa_rugosa Rorug04G0073400
rosa_samantha Rh4AG140700 Rh4CG147600 Rh4DG134700
rosa_wichuraiana Rw4G011450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 314
AasI GACNNNNNNGTC 1 cut(s) 536
Acc36I ACCTGC 1 cut(s) 314
AccI GTMKAC 1 cut(s) 540
AccII CGCG 1 cut(s) 282
AciI CCGC 4 cut(s) 280, 304, 716, 726
AclI AACGTT 1 cut(s) 170
AclWI GGATC 2 cut(s) 777, 790
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 572
AfaI GTAC 1 cut(s) 548
AgsI TTSAA 3 cut(s) 162, 734, 766
AjnI CCWGG 1 cut(s) 480
AloI GAACNNNNNNTCC 2 cut(s) 693, 725
AluBI AGCT 5 cut(s) 526, 560, 586, 749, 825
AluI AGCT 5 cut(s) 526, 560, 586, 749, 825
Alw21I GWGCWC 2 cut(s) 588, 810
Alw26I GTCTC 3 cut(s) 184, 290, 409
AlwI GGATC 2 cut(s) 777, 790
AlwNI CAGNNNCTG 1 cut(s) 649
Ama87I CYCGRG 1 cut(s) 602
AoxI GGCC 2 cut(s) 3, 723
ApeKI GCWGC 3 cut(s) 256, 332, 746
ApoI RAATTY 1 cut(s) 572
ArsI GACNNNNNNTTYG 2 cut(s) 613, 645
AspS9I GGNCC 3 cut(s) 367, 639, 723
AsuHPI GGTGA 4 cut(s) 29, 125, 338, 481
AsuII TTCGAA 1 cut(s) 468
AvaI CYCGRG 1 cut(s) 602
AvaII GGWCC 2 cut(s) 367, 639
BamHI GGATCC 1 cut(s) 782
BanII GRGCYC 1 cut(s) 588
BarI GAAGNNNNNNTAC 2 cut(s) 690, 722
BauI CACGAG 1 cut(s) 149
Bbv12I GWGCWC 2 cut(s) 588, 810
BbvI GCAGC 3 cut(s) 268, 344, 758
BccI CCATC 2 cut(s) 675, 773
BceAI ACGGC 2 cut(s) 713, 719
BciT130I CCWGG 1 cut(s) 482
BciVI GTATCC 1 cut(s) 341
BcoDI GTCTC 3 cut(s) 184, 290, 409
BfaI CTAG 1 cut(s) 750
BfmI CTRYAG 3 cut(s) 251, 553, 820
BfuAI ACCTGC 1 cut(s) 314
BfuI GTATCC 1 cut(s) 341
BisI GCNGC 5 cut(s) 257, 304, 333, 726, 747
BlsI GCNGC 5 cut(s) 258, 305, 334, 727, 748
Bme1390I CCNGG 1 cut(s) 482
Bme18I GGWCC 2 cut(s) 367, 639
BmeT110I CYCGRG 1 cut(s) 602
BmgT120I GGNCC 3 cut(s) 367, 639, 723
BmiI GGNNCC 3 cut(s) 640, 641, 784
BmrFI CCNGG 1 cut(s) 482
BmrI ACTGGG 1 cut(s) 637
BmuI ACTGGG 1 cut(s) 637
BpmI CTGGAG 1 cut(s) 433
Bpu14I TTCGAA 1 cut(s) 468
BsaJI CCNNGG 3 cut(s) 363, 534, 603
BsaXI ACNNNNNCTCC 2 cut(s) 693, 723
Bse1I ACTGG 3 cut(s) 370, 544, 643
BseBI CCWGG 1 cut(s) 482
BseDI CCNNGG 3 cut(s) 363, 534, 603
BseGI GGATG 4 cut(s) 375, 522, 667, 784
BseNI ACTGG 3 cut(s) 370, 544, 643
BseRI GAGGAG 4 cut(s) 103, 211, 214, 299
BseXI GCAGC 3 cut(s) 268, 344, 758
Bsh1236I CGCG 1 cut(s) 282
BshFI GGCC 2 cut(s) 5, 725
BsiHKAI GWGCWC 2 cut(s) 588, 810
BsiHKCI CYCGRG 1 cut(s) 602
BslFI GGGAC 1 cut(s) 652
BsmAI GTCTC 3 cut(s) 184, 290, 409
BsmFI GGGAC 1 cut(s) 652
BsmI GAATGC 1 cut(s) 631
BsnI GGCC 2 cut(s) 5, 725
BsoBI CYCGRG 1 cut(s) 602
Bsp119I TTCGAA 1 cut(s) 468
Bsp1286I GDGCHC 2 cut(s) 588, 810
Bsp143I GATC 2 cut(s) 153, 782
BspACI CCGC 4 cut(s) 280, 304, 716, 726
BspANI GGCC 2 cut(s) 5, 725
BspFNI CGCG 1 cut(s) 282
BspHI TCATGA 1 cut(s) 52
BspLI GGNNCC 3 cut(s) 640, 641, 784
BspMI ACCTGC 1 cut(s) 314
BspPI GGATC 2 cut(s) 777, 790
BspT104I TTCGAA 1 cut(s) 468
BsrI ACTGG 3 cut(s) 370, 544, 643
BssECI CCNNGG 3 cut(s) 363, 534, 603
BssMI GATC 2 cut(s) 153, 782
BssSI CACGAG 1 cut(s) 149
Bst2BI CACGAG 1 cut(s) 149
Bst2UI CCWGG 1 cut(s) 482
Bst4CI ACNGT 2 cut(s) 106, 178
Bst6I CTCTTC 4 cut(s) 130, 163, 288, 449
BstBI TTCGAA 1 cut(s) 468
BstC8I GCNNGC 1 cut(s) 718
BstF5I GGATG 4 cut(s) 375, 522, 667, 784
BstFNI CGCG 1 cut(s) 282
BstKTI GATC 2 cut(s) 156, 785
BstMAI GTCTC 3 cut(s) 184, 290, 409
BstMBI GATC 2 cut(s) 153, 782
BstMWI GCNNNNNNNGC 2 cut(s) 329, 722
BstNI CCWGG 1 cut(s) 482
BstSCI CCNGG 1 cut(s) 480
BstSFI CTRYAG 3 cut(s) 251, 553, 820
BstUI CGCG 1 cut(s) 282
BstV1I GCAGC 3 cut(s) 268, 344, 758
BstX2I RGATCY 1 cut(s) 782
BstYI RGATCY 1 cut(s) 782
BsuI GTATCC 1 cut(s) 341
BsuRI GGCC 2 cut(s) 5, 725
BtsCI GGATG 4 cut(s) 375, 522, 667, 784
BveI ACCTGC 1 cut(s) 314
Cac8I GCNNGC 1 cut(s) 718
CaiI CAGNNNCTG 1 cut(s) 649
CciI TCATGA 1 cut(s) 52
Cfr13I GGNCC 3 cut(s) 367, 639, 723
Csp6I GTAC 1 cut(s) 547
CviAII CATG 1 cut(s) 53
CviQI GTAC 1 cut(s) 547
DpnI GATC 2 cut(s) 155, 784
DpnII GATC 2 cut(s) 153, 782
DrdI GACNNNNNNGTC 1 cut(s) 536
DseDI GACNNNNNNGTC 1 cut(s) 536
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 130, 163, 288, 449
EarI CTCTTC 4 cut(s) 130, 163, 288, 449
Ecl136II GAGCTC 1 cut(s) 586
Eco24I GRGCYC 1 cut(s) 588
Eco47I GGWCC 2 cut(s) 367, 639
Eco53kI GAGCTC 1 cut(s) 586
Eco88I CYCGRG 1 cut(s) 602
EcoICRI GAGCTC 1 cut(s) 586
EcoO109I RGGNCCY 1 cut(s) 639
EcoRI GAATTC 1 cut(s) 572
EcoRII CCWGG 1 cut(s) 480
EcoT38I GRGCYC 1 cut(s) 588
FaeI CATG 1 cut(s) 56
FaiI YATR 3 cut(s) 28, 54, 822
FaqI GGGAC 1 cut(s) 652
FatI CATG 1 cut(s) 52
FauI CCCGC 1 cut(s) 709
FblI GTMKAC 1 cut(s) 540
Fnu4HI GCNGC 5 cut(s) 257, 304, 333, 726, 747
FokI GGATG 4 cut(s) 362, 529, 654, 791
FriOI GRGCYC 1 cut(s) 588
Fsp4HI GCNGC 5 cut(s) 257, 304, 333, 726, 747
FspBI CTAG 1 cut(s) 750
GluI GCNGC 5 cut(s) 257, 304, 333, 726, 747
GsuI CTGGAG 1 cut(s) 433
HaeIII GGCC 2 cut(s) 5, 725
Hin1II CATG 1 cut(s) 56
HincII GTYRAC 3 cut(s) 10, 478, 541
HindII GTYRAC 3 cut(s) 10, 478, 541
HinfI GANTC 3 cut(s) 202, 298, 812
HpaI GTTAAC 1 cut(s) 478
HphI GGTGA 4 cut(s) 29, 125, 338, 481
Hpy166II GTNNAC 4 cut(s) 10, 174, 478, 541
Hpy188I TCNGA 4 cut(s) 364, 663, 795, 817
Hpy188III TCNNGA 4 cut(s) 53, 151, 412, 451
Hpy8I GTNNAC 4 cut(s) 10, 174, 478, 541
Hpy99I CGWCG 2 cut(s) 293, 596
HpyAV CCTTC 5 cut(s) 149, 216, 250, 728, 760
HpyCH4III ACNGT 2 cut(s) 106, 178
HpyCH4IV ACGT 2 cut(s) 170, 351
HpyF10VI GCNNNNNNNGC 2 cut(s) 329, 722
HpySE526I ACGT 2 cut(s) 170, 351
Hsp92II CATG 1 cut(s) 56
KflI GGGWCCC 1 cut(s) 639
KspAI GTTAAC 1 cut(s) 478
Kzo9I GATC 2 cut(s) 153, 782
LmnI GCTCC 1 cut(s) 583
Lsp1109I GCAGC 3 cut(s) 268, 344, 758
MaeI CTAG 1 cut(s) 750
MaeII ACGT 2 cut(s) 170, 351
MaeIII GTNAC 1 cut(s) 106
MalI GATC 2 cut(s) 155, 784
MboI GATC 2 cut(s) 153, 782
MflI RGATCY 1 cut(s) 782
MhlI GDGCHC 2 cut(s) 588, 810
MluCI AATT 1 cut(s) 572
MlyI GAGTC 2 cut(s) 196, 292
MmeI TCCRAC 1 cut(s) 258
MseI TTAA 4 cut(s) 87, 477, 678, 773
MspR9I CCNGG 1 cut(s) 482
Mva1269I GAATGC 1 cut(s) 631
MvaI CCWGG 1 cut(s) 482
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 2 cut(s) 329, 722
NdeII GATC 2 cut(s) 153, 782
NlaIII CATG 1 cut(s) 56
NlaIV GGNNCC 3 cut(s) 640, 641, 784
NspV TTCGAA 1 cut(s) 468
PagI TCATGA 1 cut(s) 52
PaqCI CACCTGC 1 cut(s) 314
PcsI WCGNNNNNNNCGW 2 cut(s) 444, 600
PctI GAATGC 1 cut(s) 631
PfeI GAWTC 1 cut(s) 812
PkrI GCNGC 5 cut(s) 258, 305, 334, 727, 748
PleI GAGTC 2 cut(s) 196, 292
PpsI GAGTC 2 cut(s) 196, 292
PpuMI RGGWCCY 1 cut(s) 639
Psp124BI GAGCTC 1 cut(s) 588
Psp1406I AACGTT 1 cut(s) 170
Psp5II RGGWCCY 1 cut(s) 639
Psp6I CCWGG 1 cut(s) 480
PspGI CCWGG 1 cut(s) 480
PspN4I GGNNCC 3 cut(s) 640, 641, 784
PspPI GGNCC 3 cut(s) 367, 639, 723
PspPPI RGGWCCY 1 cut(s) 639
PstNI CAGNNNCTG 1 cut(s) 649
PsuI RGATCY 1 cut(s) 782
RsaI GTAC 1 cut(s) 548
RsaNI GTAC 1 cut(s) 547
SacI GAGCTC 1 cut(s) 588
SalI GTCGAC 1 cut(s) 539
SaqAI TTAA 4 cut(s) 87, 477, 678, 773
SatI GCNGC 5 cut(s) 257, 304, 333, 726, 747
Sau3AI GATC 2 cut(s) 153, 782
Sau96I GGNCC 3 cut(s) 367, 639, 723
SchI GAGTC 2 cut(s) 196, 292
ScrFI CCNGG 1 cut(s) 482
SduI GDGCHC 2 cut(s) 588, 810
SfcI CTRYAG 3 cut(s) 251, 553, 820
SfuI TTCGAA 1 cut(s) 468
SinI GGWCC 2 cut(s) 367, 639
Sse9I AATT 1 cut(s) 572
SsiI CCGC 4 cut(s) 280, 304, 716, 726
SspMI CTAG 1 cut(s) 750
SstI GAGCTC 1 cut(s) 588
StyD4I CCNGG 1 cut(s) 480
TaaI ACNGT 2 cut(s) 106, 178
TaiI ACGT 2 cut(s) 173, 354
TaqI TCGA 6 cut(s) 200, 243, 468, 528, 540, 810
TasI AATT 1 cut(s) 572
TatI WGTACW 1 cut(s) 546
TauI GCSGC 2 cut(s) 306, 728
TfiI GAWTC 1 cut(s) 812
Tru1I TTAA 4 cut(s) 87, 477, 678, 773
Tru9I TTAA 4 cut(s) 87, 477, 678, 773
TseI GCWGC 3 cut(s) 256, 332, 746
TspDTI ATGAA 4 cut(s) 364, 490, 533, 565
TspGWI ACGGA 1 cut(s) 436
VpaK11BI GGWCC 2 cut(s) 367, 639
XapI RAATTY 1 cut(s) 572
XmiI GTMKAC 1 cut(s) 540
XspI CTAG 1 cut(s) 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.