Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0010789

Physical Location & Seq

Genomic Coordinates Forward (+)

9412451 .. 9417763

Gene Structure 5313 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1069000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1734 bp

ATGGGTTTTGTGTGGTGGAGATTGTTTGTTGCTGCAGTTGTTGTAATTCTTTTCTTTCATGGTTTTTCTAGCGCTGAAAGAGCCCCAAACTTCACCTTCATGCATAGCGCAACAACAGCTCCCGACATATCATACTATGACTACATTATAGTGGGTGGTGGCACTGCTGGCTGCCCCTTGGCCGCCAGTCTTTCCCAAAATTACAGCGTTTTGCTACTTGAACGCGGCGGCTCTCCCTATGGCAACCCCAACATTACAAATTTGTCTACCTTTGGCAGTGCACTCTCTGATCTCTCCCCTGCCTCCCCTTCCCAGCGCTTCATCTCTGAGGATGGTGTCATTAACGCCCGCGCCCGTGTGCTAGGTGGCGGGAGCTGTCTCAATGCCGGTTTTTACACTCGCGCTCCCCCTGACTACATTAGGGAAGCTGCATGGGACGGGAGATTAGTTAACGAATCATACCAATGGGTAGAGCACCTTGTGGCGTTTCGGCCGCCAATTCAGGCATGGCAATCGGCGGTGCGCAATGGTTTGATGGAAGCTGGGGTGATGCCTTACAATGGGTTCACTTATGACCATATCTACGGGACGAAGGTTGGAGGAACCATTTTTGATCTGGAGGGACATAGACACACTGCTGCTGATTTATTAGAGTACGCTAACCCCACTGGACTTACTGTGCTCTTGCATGCTGCTGTGCATAAGATCTTGTTTAGAACCAAAGGAAAATCAAGGCCACTGGCCCATGGGGTGACGTTCAGAGACTCCTCAGGGACCAAGCACAGAGCCTATCTAAAAAACGGGTCCAAAAACGAGATCATTATATCATCTGGTGCGCTCGGAAGCCCACAGCTTCTGATGCTGAGTGGAGTGGGCCCAGCAGAACAACTTAAGGCCCACAACATCACAGTGGTGGTGGACCAGCCCATGGTTGGGCAAGGCATGTCTGACAACCCCATGAATGCAATTTTTGTGCCCTCCCCAATCCCTGTGGAGGTCTCACTGATTCAGGTGGTAGGGATCACCCACTTTGGAAGCTACATTGAAGCTGCCAGTGGTGAAAACTTTGCTGGCGGTTCTACTACCAAAGACTTTGGAATGTTCTCTCCCAAGATTGGTCAGCTCTCCACAGTGCCACCAAAGCAAAGGACCCCAGAAGCCCTAGCCGAAGCTGTAAGATACATGGACAGTCTTGAAGACGCAGCCTTCAAAGGAGGGTTCATTCTTGAGAAAGTCATGGGTCCAATTTCCACAGGCCACATGGAGCTCCGGACCCGAAACCCGAACGACAACCCGGCGGTCACATTCAACTACTTCAAAGAGCCCCAAGACTTGGAGAGATGTGTCCAAGGCATTGAAACCATTGAGAAAATAATAGAATCAAAGGCCTTCTCTAAGTTCAGATACGAGGGTATGTCAATAGCAGCTCTGCTGAACGCTACTGCAAGTAGCCCTGTAAATTTGTTGCCTAAACATGCCAATGCGTCAAGGTCTTTGGATCAGTTTTGCAAGGACACTGTCATGACCATATGGCATTACCATGGAGGATGCCAAGTTGGTAGGGTTGTTGACCATGATTACAAGGTTCTTGGTGTCGATGCTCTTCGAGTTATTGACGGTTCGACGTTTAATTATTCCCCCGGAACCAATCCTCAAGCCACTGTTATGATGCTTGGAAGGTACATGGGAAAAACAATATTGGATGAGAGGCTTGCTAGTGATGTATCAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005576 GO:0006082 GO:0006629 GO:0006631 GO:0007154 GO:0007267 GO:0007275 GO:0008150 GO:0008152 GO:0009553 GO:0009987 GO:0010430 GO:0016829 GO:0016830 GO:0016832 GO:0019395 GO:0019752 GO:0023052 GO:0030258 GO:0032501 GO:0032502 GO:0032787 GO:0034440 GO:0036094 GO:0043167 GO:0043168 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0046593 GO:0048037 GO:0048229 GO:0048856 GO:0050660 GO:0050662 GO:0055114 GO:0071704 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00260 ko01100 map00260 map01100

Pfam Domains

Protein Families

GMC_oxred_C GMC_oxred_N

Protein Analysis

578

Amino Acids

62.65

Weight (kDa)

6.63

Isoelectric Point (pI)

40.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GMC_oxred_N	PF00732	46 - 319	2.2e-18	GMC oxidoreductase
GMC_oxred_C	PF05199	415 - 559	3.6e-28	GMC oxidoreductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010789)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12570
fragaria_vesca	FvH4_5g01380
malus_domestica	MD04G1069000.v1.1
prunus_persica	Prupe.5G089100_v2.0.a1
pyrus_communis	pycom04g06280
rosa_chinensis	RchiOBHm_Chr7g0199501
rosa_laevigata	RLG00000003788
rosa_multiflora	Rmu_co8367015.1_g000001 Rmu_sc0003549.1_g000002
rosa_roxburghii	Rroxscaffold_3G00256670
rosa_rugosa	Rorug07G0049400 Rorug07G0055000 Rorug07G0055100 Rorug07G0055200
rosa_samantha	Rh7AG179700 Rh7BG176500 Rh7BG183000 Rh7CG188500 Rh7DG182400
rosa_wichuraiana	Rw7G015780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	524
AccB7I	CCANNNNNTGG	2 cut(s)	928, 1333
AccI	GTMKAC	1 cut(s)	266
AccII	CGCG	3 cut(s)	225, 351, 402
AccIII	TCCGGA	1 cut(s)	1269
AciI	CCGC	9 cut(s)	183, 225, 228, 349, 369, 494, 518, 1074, 1298
AclWI	GGATC	2 cut(s)	1028, 1506
AcoI	YGGCCR	2 cut(s)	180, 491
AcsI	RAATTY	2 cut(s)	259, 1459
AdeI	CACNNNGTG	1 cut(s)	481
AfaI	GTAC	2 cut(s)	656, 1682
AfeI	AGCGCT	2 cut(s)	73, 317
AfiI	CCNNNNNNNGG	7 cut(s)	560, 928, 932, 933, 994, 1115, 1333
AflII	CTTAAG	1 cut(s)	890
AgsI	TTSAA	7 cut(s)	221, 1046, 1196, 1210, 1309, 1318, 1358
AleI	CACNNNNGTG	1 cut(s)	911
Alw21I	GWGCWC	4 cut(s)	283, 477, 684, 1269
Alw26I	GTCTC	3 cut(s)	383, 756, 1003
Alw44I	GTGCAC	1 cut(s)	279
AlwI	GGATC	2 cut(s)	1028, 1506
AlwNI	CAGNNNCTG	1 cut(s)	856
Aor13HI	TCCGGA	1 cut(s)	1269
Aor51HI	AGCGCT	2 cut(s)	73, 317
AoxI	GGCC	8 cut(s)	180, 491, 734, 741, 874, 894, 1255, 1386
ApaI	GGGCCC	1 cut(s)	878
ApaLI	GTGCAC	1 cut(s)	279
ApeKI	GCWGC	8 cut(s)	32, 171, 428, 638, 692, 1049, 1202, 1424
ApoI	RAATTY	2 cut(s)	259, 1459
AspLEI	GCGC	7 cut(s)	74, 110, 318, 353, 404, 525, 838
AsuC2I	CCSGG	2 cut(s)	1295, 1641
AsuHPI	GGTGA	5 cut(s)	85, 559, 763, 1015, 1070
AvaII	GGWCC	6 cut(s)	774, 804, 919, 1149, 1241, 1272
AxyI	CCTNAGG	1 cut(s)	769
BaeGI	GKGCMC	3 cut(s)	283, 878, 978
BaeI	ACNNNNGTAYC	2 cut(s)	1396, 1429
BanII	GRGCYC	4 cut(s)	85, 878, 1269, 1326
BbsI	GAAGAC	1 cut(s)	1203
Bbv12I	GWGCWC	4 cut(s)	283, 477, 684, 1269
BbvI	GCAGC	8 cut(s)	19, 158, 415, 625, 679, 1036, 1214, 1436
BccI	CCATC	2 cut(s)	326, 529
BcgI	CGANNNNNNTGC	2 cut(s)	495, 529
BcnI	CCSGG	2 cut(s)	1295, 1641
BcoDI	GTCTC	3 cut(s)	383, 756, 1003
BfaI	CTAG	4 cut(s)	69, 362, 1163, 1716
BfmI	CTRYAG	1 cut(s)	33
BfoI	RGCGCY	2 cut(s)	75, 319
BfrI	CTTAAG	1 cut(s)	890
BglII	AGATCT	1 cut(s)	705
Bme1390I	CCNGG	2 cut(s)	1295, 1641
Bme18I	GGWCC	6 cut(s)	774, 804, 919, 1149, 1241, 1272
BmiI	GGNNCC	8 cut(s)	604, 775, 805, 876, 1151, 1242, 1274, 1645
BmrFI	CCNGG	2 cut(s)	1295, 1641
BmsI	GCATC	5 cut(s)	540, 849, 1538, 1588, 1659
BpiI	GAAGAC	1 cut(s)	1203
BpmI	CTGGAG	1 cut(s)	638
BpuEI	CTTGAG	2 cut(s)	1247, 1638
BpuMI	CCSGG	2 cut(s)	1295, 1641
BsaI	GGTCTC	1 cut(s)	1003
BsaJI	CCNNGG	6 cut(s)	177, 745, 927, 1348, 1540, 1639
BsaWI	WCCGGW	1 cut(s)	1269
BsaXI	ACNNNNNCTCC	4 cut(s)	103, 133, 388, 418
Bsc4I	CCNNNNNNNGG	7 cut(s)	560, 928, 932, 933, 994, 1115, 1333
Bse118I	RCCGGY	1 cut(s)	386
Bse1I	ACTGG	4 cut(s)	186, 673, 744, 1053
Bse21I	CCTNAGG	1 cut(s)	769
Bse3DI	GCAATG	1 cut(s)	532
BseAI	TCCGGA	1 cut(s)	1269
BseDI	CCNNGG	6 cut(s)	177, 745, 927, 1348, 1540, 1639
BseGI	GGATG	3 cut(s)	337, 1553, 1708
BseLI	CCNNNNNNNGG	7 cut(s)	560, 928, 932, 933, 994, 1115, 1333
BseMI	GCAATG	1 cut(s)	532
BseMII	CTCAG	3 cut(s)	318, 783, 854
BseNI	ACTGG	4 cut(s)	186, 673, 744, 1053
BseRI	GAGGAG	1 cut(s)	757
BseSI	GKGCMC	3 cut(s)	283, 878, 978
BseX3I	CGGCCG	1 cut(s)	491
BseXI	GCAGC	8 cut(s)	19, 158, 415, 625, 679, 1036, 1214, 1436
BseYI	CCCAGC	3 cut(s)	312, 542, 877
Bsh1236I	CGCG	3 cut(s)	225, 351, 402
Bsh1285I	CGRYCG	1 cut(s)	494
BshFI	GGCC	8 cut(s)	182, 493, 736, 743, 876, 896, 1257, 1388
BsiEI	CGRYCG	1 cut(s)	494
BsiHKAI	GWGCWC	4 cut(s)	283, 477, 684, 1269
BsiSI	CCGG	4 cut(s)	387, 1270, 1295, 1641
BslFI	GGGAC	4 cut(s)	449, 601, 636, 787
BslI	CCNNNNNNNGG	7 cut(s)	560, 928, 932, 933, 994, 1115, 1333
BsmAI	GTCTC	3 cut(s)	383, 756, 1003
BsmFI	GGGAC	4 cut(s)	449, 601, 636, 787
BsmI	GAATGC	1 cut(s)	967
BsnI	GGCC	8 cut(s)	182, 493, 736, 743, 876, 896, 1257, 1388
Bso31I	GGTCTC	1 cut(s)	1003
Bsp120I	GGGCCC	1 cut(s)	874
Bsp1286I	GDGCHC	8 cut(s)	85, 283, 477, 684, 878, 978, 1269, 1326
Bsp13I	TCCGGA	1 cut(s)	1269
Bsp143I	GATC	6 cut(s)	289, 613, 705, 816, 1020, 1498
Bsp19I	CCATGG	3 cut(s)	745, 927, 1540
BspACI	CCGC	9 cut(s)	183, 225, 228, 349, 369, 494, 518, 1074, 1298
BspANI	GGCC	8 cut(s)	182, 493, 736, 743, 876, 896, 1257, 1388
BspCNI	CTCAG	3 cut(s)	319, 782, 855
BspEI	TCCGGA	1 cut(s)	1269
BspFNI	CGCG	3 cut(s)	225, 351, 402
BspHI	TCATGA	1 cut(s)	1521
BspLI	GGNNCC	8 cut(s)	604, 775, 805, 876, 1151, 1242, 1274, 1645
BspMAI	CTGCAG	1 cut(s)	37
BspPI	GGATC	2 cut(s)	1028, 1506
BspQI	GCTCTTC	1 cut(s)	1608
BspTI	CTTAAG	1 cut(s)	890
BspTNI	GGTCTC	1 cut(s)	1003
BsrDI	GCAATG	1 cut(s)	532
BsrFI	RCCGGY	1 cut(s)	386
BsrI	ACTGG	4 cut(s)	186, 673, 744, 1053
BssAI	RCCGGY	1 cut(s)	386
BssECI	CCNNGG	6 cut(s)	177, 745, 927, 1348, 1540, 1639
BssMI	GATC	6 cut(s)	289, 613, 705, 816, 1020, 1498
BssT1I	CCWWGG	5 cut(s)	177, 745, 927, 1348, 1540
Bst4CI	ACNGT	7 cut(s)	679, 910, 1132, 1190, 1519, 1619, 1663
Bst6I	CTCTTC	1 cut(s)	1608
BstAFI	CTTAAG	1 cut(s)	890
BstC8I	GCNNGC	5 cut(s)	169, 349, 690, 1072, 1713
BstDEI	CTNAG	4 cut(s)	327, 769, 863, 1395
BstDSI	CCRYGG	3 cut(s)	745, 927, 1540
BstF5I	GGATG	3 cut(s)	337, 1553, 1708
BstFNI	CGCG	3 cut(s)	225, 351, 402
BstH2I	RGCGCY	2 cut(s)	75, 319
BstHHI	GCGC	7 cut(s)	74, 110, 318, 353, 404, 525, 838
BstKTI	GATC	6 cut(s)	292, 616, 708, 819, 1023, 1501
BstMAI	GTCTC	3 cut(s)	383, 756, 1003
BstMBI	GATC	6 cut(s)	289, 613, 705, 816, 1020, 1498
BstMCI	CGRYCG	1 cut(s)	494
BstMWI	GCNNNNNNNGC	5 cut(s)	80, 116, 168, 859, 1141
BstNSI	RCATGY	3 cut(s)	692, 946, 1478
BstSCI	CCNGG	2 cut(s)	1293, 1639
BstSFI	CTRYAG	1 cut(s)	33
BstSLI	GKGCMC	3 cut(s)	283, 878, 978
BstUI	CGCG	3 cut(s)	225, 351, 402
BstV1I	GCAGC	8 cut(s)	19, 158, 415, 625, 679, 1036, 1214, 1436
BstV2I	GAAGAC	1 cut(s)	1203
BstX2I	RGATCY	1 cut(s)	705
BstYI	RGATCY	1 cut(s)	705
BstZI	CGGCCG	1 cut(s)	491
Bsu36I	CCTNAGG	1 cut(s)	769
BsuRI	GGCC	8 cut(s)	182, 493, 736, 743, 876, 896, 1257, 1388
BtgI	CCRYGG	3 cut(s)	745, 927, 1540
BtsCI	GGATG	3 cut(s)	337, 1553, 1708
BtsI	GCAGTG	3 cut(s)	162, 283, 633
Cac8I	GCNNGC	5 cut(s)	169, 349, 690, 1072, 1713
CaiI	CAGNNNCTG	1 cut(s)	856
CciI	TCATGA	1 cut(s)	1521
CfoI	GCGC	7 cut(s)	74, 110, 318, 353, 404, 525, 838
Cfr10I	RCCGGY	1 cut(s)	386
CseI	GACGC	2 cut(s)	1208, 1473
Csp6I	GTAC	2 cut(s)	655, 1681
CspCI	CAANNNNNGTGG	2 cut(s)	972, 1007
CviQI	GTAC	2 cut(s)	655, 1681
DdeI	CTNAG	4 cut(s)	327, 769, 863, 1395
DpnI	GATC	6 cut(s)	291, 615, 707, 818, 1022, 1500
DpnII	GATC	6 cut(s)	289, 613, 705, 816, 1020, 1498
DraIII	CACNNNGTG	1 cut(s)	481
EaeI	YGGCCR	2 cut(s)	180, 491
EagI	CGGCCG	1 cut(s)	491
Eam1104I	CTCTTC	1 cut(s)	1608
EarI	CTCTTC	1 cut(s)	1608
Ecl136II	GAGCTC	1 cut(s)	1267
EclXI	CGGCCG	1 cut(s)	491
Eco130I	CCWWGG	5 cut(s)	177, 745, 927, 1348, 1540
Eco147I	AGGCCT	1 cut(s)	1388
Eco24I	GRGCYC	4 cut(s)	85, 878, 1269, 1326
Eco31I	GGTCTC	1 cut(s)	1003
Eco47I	GGWCC	6 cut(s)	774, 804, 919, 1149, 1241, 1272
Eco47III	AGCGCT	2 cut(s)	73, 317
Eco52I	CGGCCG	1 cut(s)	491
Eco53kI	GAGCTC	1 cut(s)	1267
Eco81I	CCTNAGG	1 cut(s)	769
EcoICRI	GAGCTC	1 cut(s)	1267
EcoO109I	RGGNCCY	1 cut(s)	1149
EcoT14I	CCWWGG	5 cut(s)	177, 745, 927, 1348, 1540
EcoT22I	ATGCAT	1 cut(s)	105
EcoT38I	GRGCYC	4 cut(s)	85, 878, 1269, 1326
ErhI	CCWWGG	5 cut(s)	177, 745, 927, 1348, 1540
FaqI	GGGAC	4 cut(s)	449, 601, 636, 787
FauI	CCCGC	2 cut(s)	356, 362
FauNDI	CATATG	1 cut(s)	1529
FblI	GTMKAC	1 cut(s)	266
FokI	GGATG	3 cut(s)	344, 1560, 1715
FriOI	GRGCYC	4 cut(s)	85, 878, 1269, 1326
FspBI	CTAG	4 cut(s)	69, 362, 1163, 1716
FspI	TGCGCA	1 cut(s)	524
GlaI	GCGC	7 cut(s)	73, 109, 317, 352, 403, 524, 837
GsaI	CCCAGC	3 cut(s)	316, 546, 881
GsuI	CTGGAG	1 cut(s)	638
HaeII	RGCGCY	2 cut(s)	75, 319
HaeIII	GGCC	8 cut(s)	182, 493, 736, 743, 876, 896, 1257, 1388
HapII	CCGG	4 cut(s)	387, 1270, 1295, 1641
HgaI	GACGC	2 cut(s)	1208, 1473
HhaI	GCGC	7 cut(s)	74, 110, 318, 353, 404, 525, 838
Hin6I	GCGC	7 cut(s)	72, 108, 316, 351, 402, 523, 836
HinP1I	GCGC	7 cut(s)	72, 108, 316, 351, 402, 523, 836
HincII	GTYRAC	2 cut(s)	451, 1570
HindII	GTYRAC	2 cut(s)	451, 1570
HinfI	GANTC	4 cut(s)	455, 764, 1006, 1379
HpaI	GTTAAC	1 cut(s)	451
HpaII	CCGG	4 cut(s)	387, 1270, 1295, 1641
HphI	GGTGA	5 cut(s)	85, 559, 763, 1015, 1070
Hpy166II	GTNNAC	6 cut(s)	267, 281, 451, 567, 919, 1570
Hpy188I	TCNGA	7 cut(s)	289, 328, 761, 842, 858, 949, 1403
Hpy188III	TCNNGA	6 cut(s)	122, 617, 1193, 1226, 1270, 1522
Hpy8I	GTNNAC	6 cut(s)	267, 281, 451, 567, 919, 1570
Hpy99I	CGWCG	1 cut(s)	1627
HpyAV	CCTTC	6 cut(s)	106, 318, 586, 1216, 1399, 1671
HpyCH4III	ACNGT	7 cut(s)	679, 910, 1132, 1190, 1519, 1619, 1663
HpyCH4IV	ACGT	2 cut(s)	755, 1625
HpyCH4V	TGCA	9 cut(s)	35, 103, 281, 431, 688, 700, 965, 1445, 1509
HpyF10VI	GCNNNNNNNGC	5 cut(s)	80, 116, 168, 859, 1141
HpyF3I	CTNAG	4 cut(s)	327, 769, 863, 1395
HpySE526I	ACGT	2 cut(s)	755, 1625
HspAI	GCGC	7 cut(s)	72, 108, 316, 351, 402, 523, 836
Kpn2I	TCCGGA	1 cut(s)	1269
KspAI	GTTAAC	1 cut(s)	451
Kzo9I	GATC	6 cut(s)	289, 613, 705, 816, 1020, 1498
LguI	GCTCTTC	1 cut(s)	1608
LmnI	GCTCC	5 cut(s)	124, 372, 409, 1264, 1272
Lsp1109I	GCAGC	8 cut(s)	19, 158, 415, 625, 679, 1036, 1214, 1436
LweI	GCATC	5 cut(s)	540, 849, 1538, 1588, 1659
MaeI	CTAG	4 cut(s)	69, 362, 1163, 1716
MaeII	ACGT	2 cut(s)	755, 1625
MaeIII	GTNAC	2 cut(s)	751, 1300
MalI	GATC	6 cut(s)	291, 615, 707, 818, 1022, 1500
MboI	GATC	6 cut(s)	289, 613, 705, 816, 1020, 1498
MboII	GAAGA	2 cut(s)	1208, 1595
MflI	RGATCY	1 cut(s)	705
MhlI	GDGCHC	8 cut(s)	85, 283, 477, 684, 878, 978, 1269, 1326
MluCI	AATT	9 cut(s)	45, 199, 259, 498, 966, 1245, 1459, 1630, 1729
MlyI	GAGTC	1 cut(s)	758
MmeI	TCCRAC	1 cut(s)	577
Mph1103I	ATGCAT	1 cut(s)	105
MroI	TCCGGA	1 cut(s)	1269
MseI	TTAA	5 cut(s)	342, 450, 891, 1629, 1732
MslI	CAYNNNNRTG	9 cut(s)	98, 149, 463, 908, 911, 1479, 1520, 1539, 1664
MspCI	CTTAAG	1 cut(s)	890
MspI	CCGG	4 cut(s)	387, 1270, 1295, 1641
MspR9I	CCNGG	2 cut(s)	1295, 1641
Mva1269I	GAATGC	1 cut(s)	967
MvnI	CGCG	3 cut(s)	225, 351, 402
MwoI	GCNNNNNNNGC	5 cut(s)	80, 116, 168, 859, 1141
NciI	CCSGG	2 cut(s)	1295, 1641
NcoI	CCATGG	3 cut(s)	745, 927, 1540
NdeI	CATATG	1 cut(s)	1529
NdeII	GATC	6 cut(s)	289, 613, 705, 816, 1020, 1498
NlaIV	GGNNCC	8 cut(s)	604, 775, 805, 876, 1151, 1242, 1274, 1645
NmuCI	GTSAC	2 cut(s)	751, 1300
NsbI	TGCGCA	1 cut(s)	524
NsiI	ATGCAT	1 cut(s)	105
NspI	RCATGY	3 cut(s)	692, 946, 1478
OliI	CACNNNNGTG	1 cut(s)	911
PaeI	GCATGC	1 cut(s)	692
PagI	TCATGA	1 cut(s)	1521
PceI	AGGCCT	1 cut(s)	1388
PciSI	GCTCTTC	1 cut(s)	1608
PctI	GAATGC	1 cut(s)	967
PfeI	GAWTC	3 cut(s)	455, 1006, 1379
PflFI	GACNNNGTC	1 cut(s)	1517
PflMI	CCANNNNNTGG	2 cut(s)	928, 1333
PleI	GAGTC	1 cut(s)	758
PpsI	GAGTC	1 cut(s)	758
PpuMI	RGGWCCY	1 cut(s)	1149
Psp124BI	GAGCTC	1 cut(s)	1269
Psp5II	RGGWCCY	1 cut(s)	1149
PspFI	CCCAGC	3 cut(s)	312, 542, 877
PspN4I	GGNNCC	8 cut(s)	604, 775, 805, 876, 1151, 1242, 1274, 1645
PspOMI	GGGCCC	1 cut(s)	874
PspPPI	RGGWCCY	1 cut(s)	1149
PstI	CTGCAG	1 cut(s)	37
PstNI	CAGNNNCTG	1 cut(s)	856
PsuI	RGATCY	1 cut(s)	705
PsyI	GACNNNGTC	1 cut(s)	1517
RsaI	GTAC	2 cut(s)	656, 1682
RsaNI	GTAC	2 cut(s)	655, 1681
RseI	CAYNNNNRTG	9 cut(s)	98, 149, 463, 908, 911, 1479, 1520, 1539, 1664
SacI	GAGCTC	1 cut(s)	1269
SapI	GCTCTTC	1 cut(s)	1608
SaqAI	TTAA	5 cut(s)	342, 450, 891, 1629, 1732
Sau3AI	GATC	6 cut(s)	289, 613, 705, 816, 1020, 1498
SchI	GAGTC	1 cut(s)	758
ScrFI	CCNGG	2 cut(s)	1295, 1641
SduI	GDGCHC	8 cut(s)	85, 283, 477, 684, 878, 978, 1269, 1326
SfaNI	GCATC	5 cut(s)	540, 849, 1538, 1588, 1659
SfcI	CTRYAG	1 cut(s)	33
SinI	GGWCC	6 cut(s)	774, 804, 919, 1149, 1241, 1272
SmiMI	CAYNNNNRTG	9 cut(s)	98, 149, 463, 908, 911, 1479, 1520, 1539, 1664
SmlI	CTYRAG	3 cut(s)	890, 1226, 1653
SmoI	CTYRAG	3 cut(s)	890, 1226, 1653
SphI	GCATGC	1 cut(s)	692
Sse9I	AATT	9 cut(s)	45, 199, 259, 498, 966, 1245, 1459, 1630, 1729
SseBI	AGGCCT	1 cut(s)	1388
SsiI	CCGC	9 cut(s)	183, 225, 228, 349, 369, 494, 518, 1074, 1298
SspI	AATATT	1 cut(s)	1698
SspMI	CTAG	4 cut(s)	69, 362, 1163, 1716
SstI	GAGCTC	1 cut(s)	1269
StuI	AGGCCT	1 cut(s)	1388
StyD4I	CCNGG	2 cut(s)	1293, 1639
StyI	CCWWGG	5 cut(s)	177, 745, 927, 1348, 1540
TaaI	ACNGT	7 cut(s)	679, 910, 1132, 1190, 1519, 1619, 1663
TaiI	ACGT	2 cut(s)	758, 1628
TaqI	TCGA	3 cut(s)	1596, 1606, 1622
TasI	AATT	9 cut(s)	45, 199, 259, 498, 966, 1245, 1459, 1630, 1729
TauI	GCSGC	4 cut(s)	185, 228, 231, 496
TfiI	GAWTC	3 cut(s)	455, 1006, 1379
Tru1I	TTAA	5 cut(s)	342, 450, 891, 1629, 1732
Tru9I	TTAA	5 cut(s)	342, 450, 891, 1629, 1732
TseFI	GTSAC	2 cut(s)	751, 1300
TseI	GCWGC	8 cut(s)	32, 171, 428, 638, 692, 1049, 1202, 1424
Tsp45I	GTSAC	2 cut(s)	751, 1300
TspDTI	ATGAA	5 cut(s)	47, 88, 310, 974, 1210
Tth111I	GACNNNGTC	1 cut(s)	1517
Van91I	CCANNNNNTGG	2 cut(s)	928, 1333
Vha464I	CTTAAG	1 cut(s)	890
VneI	GTGCAC	1 cut(s)	279
VpaK11BI	GGWCC	6 cut(s)	774, 804, 919, 1149, 1241, 1272
XapI	RAATTY	2 cut(s)	259, 1459
XceI	RCATGY	3 cut(s)	692, 946, 1478
XcmI	CCANNNNNNNNNTGG	4 cut(s)	504, 613, 929, 1258
XmiI	GTMKAC	1 cut(s)	266
XspI	CTAG	4 cut(s)	69, 362, 1163, 1716
Zsp2I	ATGCAT	1 cut(s)	105

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.