Rw7G015780

HOTHEAD-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0010789
Physical Location & Seq
Forward (+)
16054078 .. 16057551
3474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G015780.1

Sequence Viewer

Length: 861 bp
ATGTTGAGTGGTGTGGGCCCTGAAGAGCACCTTAAGGCCCACAACATCACCGTTGTGTTGGACCAACCCATGGTTGGCCGAGGCATGTCAGACAACCCTATGAATGCCATTTTCGTGCCTTCACCTAGCCACGTCGAGGTGTCGCTCATCCAAGTCGTTGGCATTACTCGCTTCGGAAGCTACATTGAAGCTGCTGGAGGCGAAAACTTCTCTCCGAGGGACTATGGAATGTTATCTCCCAAGATTGGCCAGCTCTCCACCTTGCCACCAAAGCAAAGGACCCCGGAAGCCCTAGCTAAAGCTGTGGAATTCATGAACAGTCTTGATGAAGCAGCTTTTAACGGAGGCTTCATCCTTGAGAAGATCATAGGCCCCGTTTCCGCAGGCCATTTGGAGCTCCAGACCCTAAACCCGAACGACAACCCCTCTGTCACCTTCAACTATTTCCAAGACCCCCTAGACTTGGAGAGATGTGTCCAAGGCCTTCATACCATAGAGAAGGTGGTTGAATCAAAGGCATTCTCCAAATTCCGGTTCAACGATATGTCAATACCAACTCTCCTCAACATGACAGCCCGAGCCCCCATCAATTTGCTGCCTAAGCATGAGAATTCTTCAAGGTCTCTGGAGCAGTTCTGTAAGGACACTGTGATGACCATTTGGCATTACCATGGAGGATGCCAAGTTGGGAGAGTTGTTGATCATCAATACAAGGTTCTTGGTGTTGATGCTCTTAGGGTTATTGATGGCTCCACTTTTAACTACTCACCTGGGACTAATCCTCAAGCCACTGTTATGATGCTTGGAAGGTACATGGGACAGACAATACTGAATGAGAGACTTGCGACTGATGAACCATAG

Protein Analysis

286

Amino Acids

31.5

Weight (kDa)

5.71

Isoelectric Point (pI)

38.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_C PF05199 125 - 269 2.6e-27 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010789)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12570
fragaria_vesca FvH4_5g01380
malus_domestica MD04G1069000.v1.1
prunus_persica Prupe.5G089100_v2.0.a1
pyrus_communis pycom04g06280
rosa_chinensis RchiOBHm_Chr7g0199501
rosa_laevigata RLG00000003788
rosa_multiflora Rmu_co8367015.1_g000001 Rmu_sc0003549.1_g000002
rosa_roxburghii Rroxscaffold_3G00256670
rosa_rugosa Rorug07G0049400 Rorug07G0055000 Rorug07G0055100 Rorug07G0055200
rosa_samantha Rh7AG179700 Rh7BG176500 Rh7BG183000 Rh7CG188500 Rh7DG182400
rosa_wichuraiana Rw7G015780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 70
AciI CCGC 1 cut(s) 381
AcoI YGGCCR 2 cut(s) 76, 247
AcsI RAATTY 3 cut(s) 308, 527, 610
AcuI CTGAAG 1 cut(s) 42
AfaI GTAC 1 cut(s) 812
AfiI CCNNNNNNNGG 6 cut(s) 70, 74, 136, 245, 463, 531
AflII CTTAAG 1 cut(s) 32
AgsI TTSAA 5 cut(s) 188, 439, 509, 538, 618
AjiI CACGTC 1 cut(s) 133
AjnI CCWGG 1 cut(s) 769
AjuI GAANNNNNNNTTGG 2 cut(s) 518, 550
AleI CACNNNNGTG 1 cut(s) 53
AluBI AGCT 7 cut(s) 180, 191, 253, 296, 302, 335, 397
AluI AGCT 7 cut(s) 180, 191, 253, 296, 302, 335, 397
Alw21I GWGCWC 2 cut(s) 30, 399
Alw26I GTCTC 2 cut(s) 627, 832
Ama87I CYCGRG 1 cut(s) 576
AoxI GGCC 7 cut(s) 16, 36, 76, 247, 370, 385, 481
ApaI GGGCCC 1 cut(s) 20
ApeKI GCWGC 3 cut(s) 191, 332, 595
ApoI RAATTY 3 cut(s) 308, 527, 610
AspS9I GGNCC 6 cut(s) 16, 17, 37, 61, 279, 371
AsuC2I CCSGG 1 cut(s) 284
AsuHPI GGTGA 4 cut(s) 40, 114, 424, 759
AvaI CYCGRG 1 cut(s) 576
AvaII GGWCC 2 cut(s) 61, 279
BaeGI GKGCMC 1 cut(s) 20
BalI TGGCCA 1 cut(s) 249
BanII GRGCYC 3 cut(s) 20, 399, 583
Bbv12I GWGCWC 2 cut(s) 30, 399
BbvI GCAGC 3 cut(s) 178, 344, 582
BccI CCATC 2 cut(s) 593, 740
BciT130I CCWGG 1 cut(s) 771
BclI TGATCA 1 cut(s) 700
BcnI CCSGG 1 cut(s) 284
BcoDI GTCTC 2 cut(s) 627, 832
BfaI CTAG 3 cut(s) 126, 293, 458
BfrI CTTAAG 1 cut(s) 32
BisI GCNGC 3 cut(s) 192, 333, 596
BlsI GCNGC 3 cut(s) 193, 334, 597
Bme1390I CCNGG 2 cut(s) 284, 771
Bme18I GGWCC 2 cut(s) 61, 279
BmeT110I CYCGRG 1 cut(s) 576
BmgBI CACGTC 1 cut(s) 133
BmgT120I GGNCC 6 cut(s) 16, 17, 37, 61, 279, 371
BmiI GGNNCC 4 cut(s) 18, 281, 373, 751
BmrFI CCNGG 2 cut(s) 284, 771
BmsI GCATC 3 cut(s) 668, 718, 789
BpmI CTGGAG 3 cut(s) 216, 383, 647
Bpu10I CCTNAGC 1 cut(s) 600
BpuEI CTTGAG 2 cut(s) 377, 768
BpuMI CCSGG 1 cut(s) 284
BsaI GGTCTC 1 cut(s) 627
BsaJI CCNNGG 7 cut(s) 69, 79, 215, 282, 478, 670, 770
BsaWI WCCGGW 1 cut(s) 531
BsaXI ACNNNNNCTCC 2 cut(s) 543, 573
Bsc4I CCNNNNNNNGG 6 cut(s) 70, 74, 136, 245, 463, 531
BseBI CCWGG 1 cut(s) 771
BseDI CCNNGG 7 cut(s) 69, 79, 215, 282, 478, 670, 770
BseGI GGATG 3 cut(s) 147, 351, 683
BseLI CCNNNNNNNGG 6 cut(s) 70, 74, 136, 245, 463, 531
BseRI GAGGAG 1 cut(s) 551
BseSI GKGCMC 1 cut(s) 20
BseXI GCAGC 3 cut(s) 178, 344, 582
BshFI GGCC 7 cut(s) 18, 38, 78, 249, 372, 387, 483
BsiHKAI GWGCWC 2 cut(s) 30, 399
BsiHKCI CYCGRG 1 cut(s) 576
BsiSI CCGG 2 cut(s) 284, 532
BslFI GGGAC 3 cut(s) 233, 787, 831
BslI CCNNNNNNNGG 6 cut(s) 70, 74, 136, 245, 463, 531
BsmAI GTCTC 2 cut(s) 627, 832
BsmFI GGGAC 3 cut(s) 233, 787, 831
BsmI GAATGC 2 cut(s) 109, 518
BsnI GGCC 7 cut(s) 18, 38, 78, 249, 372, 387, 483
Bso31I GGTCTC 1 cut(s) 627
BsoBI CYCGRG 1 cut(s) 576
Bsp120I GGGCCC 1 cut(s) 16
Bsp1286I GDGCHC 4 cut(s) 20, 30, 399, 583
Bsp143I GATC 2 cut(s) 363, 700
Bsp19I CCATGG 2 cut(s) 69, 670
BspACI CCGC 1 cut(s) 381
BspANI GGCC 7 cut(s) 18, 38, 78, 249, 372, 387, 483
BspHI TCATGA 1 cut(s) 312
BspLI GGNNCC 4 cut(s) 18, 281, 373, 751
BspQI GCTCTTC 1 cut(s) 18
BspTI CTTAAG 1 cut(s) 32
BspTNI GGTCTC 1 cut(s) 627
BssECI CCNNGG 7 cut(s) 69, 79, 215, 282, 478, 670, 770
BssMI GATC 2 cut(s) 363, 700
BssT1I CCWWGG 3 cut(s) 69, 478, 670
Bst2UI CCWGG 1 cut(s) 771
Bst4CI ACNGT 4 cut(s) 52, 320, 649, 793
Bst6I CTCTTC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 32
BstC8I GCNNGC 2 cut(s) 251, 385
BstDEI CTNAG 2 cut(s) 600, 734
BstDSI CCRYGG 2 cut(s) 69, 670
BstF5I GGATG 3 cut(s) 147, 351, 683
BstKTI GATC 2 cut(s) 366, 703
BstMAI GTCTC 2 cut(s) 627, 832
BstMBI GATC 2 cut(s) 363, 700
BstMWI GCNNNNNNNGC 4 cut(s) 168, 177, 271, 601
BstNI CCWGG 1 cut(s) 771
BstNSI RCATGY 1 cut(s) 88
BstSCI CCNGG 2 cut(s) 282, 769
BstSLI GKGCMC 1 cut(s) 20
BstV1I GCAGC 3 cut(s) 178, 344, 582
BstXI CCANNNNNNTGG 1 cut(s) 158
BsuRI GGCC 7 cut(s) 18, 38, 78, 249, 372, 387, 483
BtgI CCRYGG 2 cut(s) 69, 670
BtrI CACGTC 1 cut(s) 133
BtsCI GGATG 3 cut(s) 147, 351, 683
BtsIMutI CAGTG 2 cut(s) 645, 789
Cac8I GCNNGC 2 cut(s) 251, 385
CciI TCATGA 1 cut(s) 312
Cfr13I GGNCC 6 cut(s) 16, 17, 37, 61, 279, 371
Csp6I GTAC 1 cut(s) 811
CviAII CATG 7 cut(s) 70, 85, 313, 568, 605, 671, 814
CviQI GTAC 1 cut(s) 811
DdeI CTNAG 2 cut(s) 600, 734
DpnI GATC 2 cut(s) 365, 702
DpnII GATC 2 cut(s) 363, 700
EaeI YGGCCR 2 cut(s) 76, 247
Eam1104I CTCTTC 1 cut(s) 18
EarI CTCTTC 1 cut(s) 18
Ecl136II GAGCTC 1 cut(s) 397
Eco130I CCWWGG 3 cut(s) 69, 478, 670
Eco147I AGGCCT 1 cut(s) 483
Eco24I GRGCYC 3 cut(s) 20, 399, 583
Eco31I GGTCTC 1 cut(s) 627
Eco47I GGWCC 2 cut(s) 61, 279
Eco53kI GAGCTC 1 cut(s) 397
Eco57I CTGAAG 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 576
EcoICRI GAGCTC 1 cut(s) 397
EcoO109I RGGNCCY 3 cut(s) 17, 279, 371
EcoRI GAATTC 2 cut(s) 308, 610
EcoRII CCWGG 1 cut(s) 769
EcoT14I CCWWGG 3 cut(s) 69, 478, 670
EcoT38I GRGCYC 3 cut(s) 20, 399, 583
ErhI CCWWGG 3 cut(s) 69, 478, 670
FaeI CATG 7 cut(s) 73, 88, 316, 571, 608, 674, 817
FalI AAGNNNNNCTT 2 cut(s) 15, 47
FaqI GGGAC 3 cut(s) 233, 787, 831
FatI CATG 7 cut(s) 69, 84, 312, 567, 604, 670, 813
FbaI TGATCA 1 cut(s) 700
Fnu4HI GCNGC 3 cut(s) 192, 333, 596
FokI GGATG 3 cut(s) 134, 338, 690
FriOI GRGCYC 3 cut(s) 20, 399, 583
Fsp4HI GCNGC 3 cut(s) 192, 333, 596
FspBI CTAG 3 cut(s) 126, 293, 458
GluI GCNGC 3 cut(s) 192, 333, 596
GsuI CTGGAG 3 cut(s) 216, 383, 647
HaeIII GGCC 7 cut(s) 18, 38, 78, 249, 372, 387, 483
HapII CCGG 2 cut(s) 284, 532
Hin1II CATG 7 cut(s) 73, 88, 316, 571, 608, 674, 817
HinfI GANTC 1 cut(s) 509
HpaII CCGG 2 cut(s) 284, 532
HphI GGTGA 4 cut(s) 40, 114, 424, 759
Hpy188I TCNGA 3 cut(s) 91, 176, 216
Hpy188III TCNNGA 4 cut(s) 313, 323, 400, 626
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 5 cut(s) 129, 445, 493, 494, 801
HpyCH4III ACNGT 4 cut(s) 52, 320, 649, 793
HpyCH4IV ACGT 1 cut(s) 132
HpyF10VI GCNNNNNNNGC 4 cut(s) 168, 177, 271, 601
HpyF3I CTNAG 2 cut(s) 600, 734
HpySE526I ACGT 1 cut(s) 132
Hsp92II CATG 7 cut(s) 73, 88, 316, 571, 608, 674, 817
Ksp22I TGATCA 1 cut(s) 700
Kzo9I GATC 2 cut(s) 363, 700
LguI GCTCTTC 1 cut(s) 18
LmnI GCTCC 4 cut(s) 394, 402, 628, 755
Lsp1109I GCAGC 3 cut(s) 178, 344, 582
LweI GCATC 3 cut(s) 668, 718, 789
MaeI CTAG 3 cut(s) 126, 293, 458
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 430
MalI GATC 2 cut(s) 365, 702
MboI GATC 2 cut(s) 363, 700
MboII GAAGA 3 cut(s) 35, 373, 606
MhlI GDGCHC 4 cut(s) 20, 30, 399, 583
MlsI TGGCCA 1 cut(s) 249
MluCI AATT 4 cut(s) 308, 527, 589, 610
MluNI TGGCCA 1 cut(s) 249
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 9 cut(s) 74, 130, 191, 210, 338, 436, 572, 668, 792
Mox20I TGGCCA 1 cut(s) 249
MscI TGGCCA 1 cut(s) 249
MseI TTAA 3 cut(s) 33, 339, 759
MslI CAYNNNNRTG 5 cut(s) 53, 113, 650, 669, 794
Msp20I TGGCCA 1 cut(s) 249
MspCI CTTAAG 1 cut(s) 32
MspI CCGG 2 cut(s) 284, 532
MspR9I CCNGG 2 cut(s) 284, 771
Mva1269I GAATGC 2 cut(s) 109, 518
MvaI CCWGG 1 cut(s) 771
MwoI GCNNNNNNNGC 4 cut(s) 168, 177, 271, 601
NciI CCSGG 1 cut(s) 284
NcoI CCATGG 2 cut(s) 69, 670
NdeII GATC 2 cut(s) 363, 700
NlaIII CATG 7 cut(s) 73, 88, 316, 571, 608, 674, 817
NlaIV GGNNCC 4 cut(s) 18, 281, 373, 751
NmeAIII GCCGAG 1 cut(s) 104
NmuCI GTSAC 1 cut(s) 430
NspI RCATGY 1 cut(s) 88
OliI CACNNNNGTG 1 cut(s) 53
PagI TCATGA 1 cut(s) 312
PceI AGGCCT 1 cut(s) 483
PciSI GCTCTTC 1 cut(s) 18
PctI GAATGC 2 cut(s) 109, 518
PfeI GAWTC 1 cut(s) 509
PflMI CCANNNNNTGG 1 cut(s) 70
PkrI GCNGC 3 cut(s) 193, 334, 597
PpuMI RGGWCCY 1 cut(s) 279
Psp124BI GAGCTC 1 cut(s) 399
Psp5II RGGWCCY 1 cut(s) 279
Psp6I CCWGG 1 cut(s) 769
PspGI CCWGG 1 cut(s) 769
PspN4I GGNNCC 4 cut(s) 18, 281, 373, 751
PspOMI GGGCCC 1 cut(s) 16
PspPI GGNCC 6 cut(s) 16, 17, 37, 61, 279, 371
PspPPI RGGWCCY 1 cut(s) 279
RsaI GTAC 1 cut(s) 812
RsaNI GTAC 1 cut(s) 811
RseI CAYNNNNRTG 5 cut(s) 53, 113, 650, 669, 794
SacI GAGCTC 1 cut(s) 399
SapI GCTCTTC 1 cut(s) 18
SaqAI TTAA 3 cut(s) 33, 339, 759
SatI GCNGC 3 cut(s) 192, 333, 596
Sau3AI GATC 2 cut(s) 363, 700
Sau96I GGNCC 6 cut(s) 16, 17, 37, 61, 279, 371
ScrFI CCNGG 2 cut(s) 284, 771
SduI GDGCHC 4 cut(s) 20, 30, 399, 583
SfaNI GCATC 3 cut(s) 668, 718, 789
SinI GGWCC 2 cut(s) 61, 279
SmiMI CAYNNNNRTG 5 cut(s) 53, 113, 650, 669, 794
SmlI CTYRAG 3 cut(s) 32, 356, 783
SmoI CTYRAG 3 cut(s) 32, 356, 783
Sse9I AATT 4 cut(s) 308, 527, 589, 610
SseBI AGGCCT 1 cut(s) 483
SsiI CCGC 1 cut(s) 381
SspMI CTAG 3 cut(s) 126, 293, 458
SstI GAGCTC 1 cut(s) 399
StuI AGGCCT 1 cut(s) 483
StyD4I CCNGG 2 cut(s) 282, 769
StyI CCWWGG 3 cut(s) 69, 478, 670
TaaI ACNGT 4 cut(s) 52, 320, 649, 793
TaiI ACGT 1 cut(s) 135
TaqI TCGA 1 cut(s) 135
TasI AATT 4 cut(s) 308, 527, 589, 610
TfiI GAWTC 1 cut(s) 509
Tru1I TTAA 3 cut(s) 33, 339, 759
Tru9I TTAA 3 cut(s) 33, 339, 759
TscAI CASTG 2 cut(s) 652, 796
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 3 cut(s) 191, 332, 595
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 6 cut(s) 116, 301, 329, 340, 342, 476
TspGWI ACGGA 1 cut(s) 357
TspRI CASTG 2 cut(s) 652, 796
Van91I CCANNNNNTGG 1 cut(s) 70
Vha464I CTTAAG 1 cut(s) 32
VpaK11BI GGWCC 2 cut(s) 61, 279
XapI RAATTY 3 cut(s) 308, 527, 610
XceI RCATGY 1 cut(s) 88
XcmI CCANNNNNNNNNTGG 2 cut(s) 71, 499
XspI CTAG 3 cut(s) 126, 293, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.