Rh7BG176500

HOTHEAD-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0010789
Physical Location & Seq
Reverse (-)
13816417 .. 13829130
12714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG176500.1

Sequence Viewer

Length: 1113 bp
ATGCCTATATATAGTGTTCCTTCTAGCTACAACGGGAGGAGAGGAGGAGAAAACGCGCTATACAGCAGAACAAAAGAAATACAAAGAAGAGGAGATGCACGAGAGAAAAAGCCACTAGAGAAAGAAAGAGAAAAGCTGCAGGCACACTGTGCCGACTTATTAGAGTATGCTAATTCCACTAGGCTCACTGTGCTCTTGCACGCCTCTGTCCATAAGATCTTGTTTAGAACAAGAGGAAAAGCAAGGCCATTGGCCCATGGTGTAGCGTTCAGAGACGCATCTGGAACCAGACACACAGCATACCTCAAGAAAGGGTCCAAGAACGAGATAATTATATCAGCTGGGGCAATTGGAAGCCCACAGATCTTGATGTTGAGTGGTGTGGGCCCTGAAGAGCACCTTAAGGCCCACAACATCACCGTTGTGTTGGACCAACGCATGGTTGGCCGAGGCATGTCAGACAACCCTATGAATGCCATTTTCGTGCCTTCACCTAGCCCCGTCGAGGTGTCGCTCATCCAAGTCGTTGGCATTATCTGCTTCGGAAGCTACATTGAAGCTGCTGGAGGCGAAAACTTCTCTCCGAGGGACTATGGAATGTTATCTCCCAAGATTGGCCAGCTCTCCACCTTGCCACCAAAGCAAAGGACCCCGGAAGCCTTAGCTAAAGCTGTGGAATTCATGAACAGTCTTGATGAAGCAGCTTTTAACGGAGGCTTCATCCTTGAGACGATCATAGGCCCCGTTTCCGCAGGCCATTTGGAGCTCCAGACCCTAAACCCGAACGACAACCCCTCTGTCACCTTCAACTATTTCCAAGACCCCCTAGACTTGGAGAGATGTGTCCGAGGCCTTCATACCATAGAGAAGGTGGTTGAATCAAAGGCATTCTCCAAATTCCGGTTCAAAGATATGTCAATACCAACTCTCCTCAACATGACAGCCCGAGCCCCCATCAATTTGCTGCCTAAGCATGAGAATTCTTCAAGGGTTATTGATGGCTCCACTTTTAACTACTCACCTGGGACTAATCCTCAAGCCACTGTTATGATGCTTGGAAGGTACATGGGACAGACAATACTGAATGAGAGACTTGCGACTGATGAACCATAG

Protein Analysis

370

Amino Acids

40.7

Weight (kDa)

9.11

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 51 - 155 2.3e-11 GMC oxidoreductase
GMC_oxred_C PF05199 248 - 298 3.5e-06 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010789)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12570
fragaria_vesca FvH4_5g01380
malus_domestica MD04G1069000.v1.1
prunus_persica Prupe.5G089100_v2.0.a1
pyrus_communis pycom04g06280
rosa_chinensis RchiOBHm_Chr7g0199501
rosa_laevigata RLG00000003788
rosa_multiflora Rmu_co8367015.1_g000001 Rmu_sc0003549.1_g000002
rosa_roxburghii Rroxscaffold_3G00256670
rosa_rugosa Rorug07G0049400 Rorug07G0055000 Rorug07G0055100 Rorug07G0055200
rosa_samantha Rh7AG179700 Rh7BG176500 Rh7BG183000 Rh7CG188500 Rh7DG182400
rosa_wichuraiana Rw7G015780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 439
AccII CGCG 1 cut(s) 56
AciI CCGC 1 cut(s) 750
AcoI YGGCCR 2 cut(s) 445, 616
AcsI RAATTY 3 cut(s) 677, 896, 979
AcuI CTGAAG 1 cut(s) 411
AdeI CACNNNGTG 1 cut(s) 149
AfaI GTAC 1 cut(s) 1064
AfiI CCNNNNNNNGG 5 cut(s) 439, 505, 614, 832, 900
AflII CTTAAG 1 cut(s) 401
AgsI TTSAA 5 cut(s) 557, 808, 878, 907, 987
AjnI CCWGG 1 cut(s) 1021
AjuI GAANNNNNNNTTGG 2 cut(s) 887, 919
AleI CACNNNNGTG 1 cut(s) 422
Alw21I GWGCWC 3 cut(s) 195, 399, 768
Alw26I GTCTC 3 cut(s) 267, 722, 1084
Ama87I CYCGRG 1 cut(s) 945
AoxI GGCC 9 cut(s) 245, 252, 385, 405, 445, 616, 739, 754, 850
ApaI GGGCCC 1 cut(s) 389
ApeKI GCWGC 4 cut(s) 136, 560, 701, 964
ApoI RAATTY 3 cut(s) 677, 896, 979
AspLEI GCGC 1 cut(s) 58
AspS9I GGNCC 8 cut(s) 253, 315, 385, 386, 406, 430, 648, 740
AsuC2I CCSGG 1 cut(s) 653
AsuHPI GGTGA 4 cut(s) 409, 483, 793, 1011
AvaI CYCGRG 1 cut(s) 945
AvaII GGWCC 3 cut(s) 315, 430, 648
BaeGI GKGCMC 1 cut(s) 389
BalI TGGCCA 1 cut(s) 618
BanII GRGCYC 3 cut(s) 389, 768, 952
BauI CACGAG 1 cut(s) 99
Bbv12I GWGCWC 3 cut(s) 195, 399, 768
BbvI GCAGC 4 cut(s) 123, 547, 713, 951
BccI CCATC 2 cut(s) 962, 992
BciT130I CCWGG 1 cut(s) 1023
BcnI CCSGG 1 cut(s) 653
BcoDI GTCTC 3 cut(s) 267, 722, 1084
BfaI CTAG 5 cut(s) 24, 116, 180, 495, 827
BfmI CTRYAG 1 cut(s) 137
BfrI CTTAAG 1 cut(s) 401
BglII AGATCT 2 cut(s) 216, 363
BisI GCNGC 4 cut(s) 137, 561, 702, 965
BlsI GCNGC 4 cut(s) 138, 562, 703, 966
Bme1390I CCNGG 2 cut(s) 653, 1023
Bme18I GGWCC 3 cut(s) 315, 430, 648
BmeT110I CYCGRG 1 cut(s) 945
BmgT120I GGNCC 8 cut(s) 253, 315, 385, 386, 406, 430, 648, 740
BmiI GGNNCC 6 cut(s) 286, 316, 387, 650, 742, 1003
BmrFI CCNGG 2 cut(s) 653, 1023
BmsI GCATC 3 cut(s) 85, 287, 1041
BpmI CTGGAG 2 cut(s) 585, 752
Bpu10I CCTNAGC 2 cut(s) 661, 969
BpuEI CTTGAG 3 cut(s) 290, 746, 1020
BpuMI CCSGG 1 cut(s) 653
BsaJI CCNNGG 6 cut(s) 256, 448, 584, 651, 847, 1022
BsaWI WCCGGW 1 cut(s) 900
BsaXI ACNNNNNCTCC 2 cut(s) 912, 942
Bsc4I CCNNNNNNNGG 5 cut(s) 439, 505, 614, 832, 900
BseBI CCWGG 1 cut(s) 1023
BseDI CCNNGG 6 cut(s) 256, 448, 584, 651, 847, 1022
BseGI GGATG 2 cut(s) 516, 720
BseLI CCNNNNNNNGG 5 cut(s) 439, 505, 614, 832, 900
BseRI GAGGAG 5 cut(s) 52, 57, 60, 105, 920
BseSI GKGCMC 1 cut(s) 389
BseXI GCAGC 4 cut(s) 123, 547, 713, 951
BseYI CCCAGC 1 cut(s) 341
Bsh1236I CGCG 1 cut(s) 56
BshFI GGCC 9 cut(s) 247, 254, 387, 407, 447, 618, 741, 756, 852
BsiHKAI GWGCWC 3 cut(s) 195, 399, 768
BsiHKCI CYCGRG 1 cut(s) 945
BsiSI CCGG 2 cut(s) 653, 901
BslFI GGGAC 3 cut(s) 602, 1039, 1083
BslI CCNNNNNNNGG 5 cut(s) 439, 505, 614, 832, 900
BsmAI GTCTC 3 cut(s) 267, 722, 1084
BsmBI CGTCTC 2 cut(s) 267, 722
BsmFI GGGAC 3 cut(s) 602, 1039, 1083
BsmI GAATGC 2 cut(s) 478, 887
BsnI GGCC 9 cut(s) 247, 254, 387, 407, 447, 618, 741, 756, 852
BsoBI CYCGRG 1 cut(s) 945
Bsp120I GGGCCC 1 cut(s) 385
Bsp1286I GDGCHC 5 cut(s) 195, 389, 399, 768, 952
Bsp143I GATC 3 cut(s) 216, 363, 732
Bsp19I CCATGG 1 cut(s) 256
BspACI CCGC 1 cut(s) 750
BspANI GGCC 9 cut(s) 247, 254, 387, 407, 447, 618, 741, 756, 852
BspFNI CGCG 1 cut(s) 56
BspHI TCATGA 1 cut(s) 681
BspLI GGNNCC 6 cut(s) 286, 316, 387, 650, 742, 1003
BspMAI CTGCAG 1 cut(s) 141
BspQI GCTCTTC 1 cut(s) 387
BspTI CTTAAG 1 cut(s) 401
BssECI CCNNGG 6 cut(s) 256, 448, 584, 651, 847, 1022
BssMI GATC 3 cut(s) 216, 363, 732
BssSI CACGAG 1 cut(s) 99
BssT1I CCWWGG 1 cut(s) 256
Bst2BI CACGAG 1 cut(s) 99
Bst2UI CCWGG 1 cut(s) 1023
Bst4CI ACNGT 5 cut(s) 149, 190, 421, 689, 1045
Bst6I CTCTTC 2 cut(s) 82, 387
BstAFI CTTAAG 1 cut(s) 401
BstAPI GCANNNNNTGC 2 cut(s) 149, 537
BstC8I GCNNGC 4 cut(s) 141, 201, 620, 754
BstDEI CTNAG 2 cut(s) 661, 969
BstDSI CCRYGG 1 cut(s) 256
BstF5I GGATG 2 cut(s) 516, 720
BstFNI CGCG 1 cut(s) 56
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 3 cut(s) 219, 366, 735
BstMAI GTCTC 3 cut(s) 267, 722, 1084
BstMBI GATC 3 cut(s) 216, 363, 732
BstMWI GCNNNNNNNGC 7 cut(s) 149, 190, 444, 537, 546, 640, 970
BstNI CCWGG 1 cut(s) 1023
BstNSI RCATGY 1 cut(s) 457
BstSCI CCNGG 2 cut(s) 651, 1021
BstSFI CTRYAG 1 cut(s) 137
BstSLI GKGCMC 1 cut(s) 389
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 4 cut(s) 123, 547, 713, 951
BstX2I RGATCY 2 cut(s) 216, 363
BstXI CCANNNNNNTGG 1 cut(s) 527
BstYI RGATCY 2 cut(s) 216, 363
BsuRI GGCC 9 cut(s) 247, 254, 387, 407, 447, 618, 741, 756, 852
BtgI CCRYGG 1 cut(s) 256
BtsCI GGATG 2 cut(s) 516, 720
BtsIMutI CAGTG 3 cut(s) 145, 186, 1041
Cac8I GCNNGC 4 cut(s) 141, 201, 620, 754
CciI TCATGA 1 cut(s) 681
CfoI GCGC 1 cut(s) 58
Cfr13I GGNCC 8 cut(s) 253, 315, 385, 386, 406, 430, 648, 740
CseI GACGC 1 cut(s) 284
Csp6I GTAC 1 cut(s) 1063
CspCI CAANNNNNGTGG 2 cut(s) 348, 383
CviAII CATG 7 cut(s) 257, 439, 454, 682, 937, 974, 1066
CviQI GTAC 1 cut(s) 1063
DdeI CTNAG 2 cut(s) 661, 969
DpnI GATC 3 cut(s) 218, 365, 734
DpnII GATC 3 cut(s) 216, 363, 732
DraIII CACNNNGTG 1 cut(s) 149
EaeI YGGCCR 2 cut(s) 445, 616
Eam1104I CTCTTC 2 cut(s) 82, 387
EarI CTCTTC 2 cut(s) 82, 387
Ecl136II GAGCTC 1 cut(s) 766
Eco130I CCWWGG 1 cut(s) 256
Eco147I AGGCCT 1 cut(s) 852
Eco24I GRGCYC 3 cut(s) 389, 768, 952
Eco47I GGWCC 3 cut(s) 315, 430, 648
Eco53kI GAGCTC 1 cut(s) 766
Eco57I CTGAAG 1 cut(s) 411
Eco88I CYCGRG 1 cut(s) 945
EcoICRI GAGCTC 1 cut(s) 766
EcoO109I RGGNCCY 3 cut(s) 386, 648, 740
EcoRI GAATTC 2 cut(s) 677, 979
EcoRII CCWGG 1 cut(s) 1021
EcoT14I CCWWGG 1 cut(s) 256
EcoT38I GRGCYC 3 cut(s) 389, 768, 952
ErhI CCWWGG 1 cut(s) 256
Esp3I CGTCTC 2 cut(s) 267, 722
FaeI CATG 7 cut(s) 260, 442, 457, 685, 940, 977, 1069
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 3 cut(s) 602, 1039, 1083
FatI CATG 7 cut(s) 256, 438, 453, 681, 936, 973, 1065
Fnu4HI GCNGC 4 cut(s) 137, 561, 702, 965
FokI GGATG 2 cut(s) 503, 707
FriOI GRGCYC 3 cut(s) 389, 768, 952
Fsp4HI GCNGC 4 cut(s) 137, 561, 702, 965
FspBI CTAG 5 cut(s) 24, 116, 180, 495, 827
GlaI GCGC 1 cut(s) 57
GluI GCNGC 4 cut(s) 137, 561, 702, 965
GsaI CCCAGC 1 cut(s) 345
GsuI CTGGAG 2 cut(s) 585, 752
HaeIII GGCC 9 cut(s) 247, 254, 387, 407, 447, 618, 741, 756, 852
HapII CCGG 2 cut(s) 653, 901
HgaI GACGC 1 cut(s) 284
HhaI GCGC 1 cut(s) 58
Hin1II CATG 7 cut(s) 260, 442, 457, 685, 940, 977, 1069
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HinfI GANTC 1 cut(s) 878
HpaII CCGG 2 cut(s) 653, 901
HphI GGTGA 4 cut(s) 409, 483, 793, 1011
Hpy188I TCNGA 5 cut(s) 272, 460, 545, 585, 848
Hpy188III TCNNGA 6 cut(s) 282, 307, 367, 682, 692, 769
Hpy99I CGWCG 1 cut(s) 506
HpyAV CCTTC 6 cut(s) 30, 498, 814, 862, 863, 1053
HpyCH4III ACNGT 5 cut(s) 149, 190, 421, 689, 1045
HpyCH4V TGCA 3 cut(s) 98, 139, 199
HpyF10VI GCNNNNNNNGC 7 cut(s) 149, 190, 444, 537, 546, 640, 970
HpyF3I CTNAG 2 cut(s) 661, 969
Hsp92II CATG 7 cut(s) 260, 442, 457, 685, 940, 977, 1069
HspAI GCGC 1 cut(s) 56
Kzo9I GATC 3 cut(s) 216, 363, 732
LguI GCTCTTC 1 cut(s) 387
LmnI GCTCC 3 cut(s) 763, 771, 1007
Lsp1109I GCAGC 4 cut(s) 123, 547, 713, 951
LweI GCATC 3 cut(s) 85, 287, 1041
MaeI CTAG 5 cut(s) 24, 116, 180, 495, 827
MaeIII GTNAC 1 cut(s) 799
MalI GATC 3 cut(s) 218, 365, 734
MboI GATC 3 cut(s) 216, 363, 732
MboII GAAGA 3 cut(s) 99, 404, 975
MfeI CAATTG 1 cut(s) 348
MflI RGATCY 2 cut(s) 216, 363
MhlI GDGCHC 5 cut(s) 195, 389, 399, 768, 952
MlsI TGGCCA 1 cut(s) 618
MluCI AATT 7 cut(s) 172, 330, 348, 677, 896, 958, 979
MluNI TGGCCA 1 cut(s) 618
MmeI TCCRAC 1 cut(s) 408
Mox20I TGGCCA 1 cut(s) 618
MscI TGGCCA 1 cut(s) 618
MseI TTAA 3 cut(s) 402, 708, 1011
MslI CAYNNNNRTG 3 cut(s) 422, 482, 1046
Msp20I TGGCCA 1 cut(s) 618
MspA1I CMGCKG 1 cut(s) 341
MspCI CTTAAG 1 cut(s) 401
MspI CCGG 2 cut(s) 653, 901
MspR9I CCNGG 2 cut(s) 653, 1023
MunI CAATTG 1 cut(s) 348
Mva1269I GAATGC 2 cut(s) 478, 887
MvaI CCWGG 1 cut(s) 1023
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 7 cut(s) 149, 190, 444, 537, 546, 640, 970
NciI CCSGG 1 cut(s) 653
NcoI CCATGG 1 cut(s) 256
NdeII GATC 3 cut(s) 216, 363, 732
NlaIII CATG 7 cut(s) 260, 442, 457, 685, 940, 977, 1069
NlaIV GGNNCC 6 cut(s) 286, 316, 387, 650, 742, 1003
NmeAIII GCCGAG 1 cut(s) 473
NmuCI GTSAC 1 cut(s) 799
NspI RCATGY 1 cut(s) 457
OliI CACNNNNGTG 1 cut(s) 422
PagI TCATGA 1 cut(s) 681
PceI AGGCCT 1 cut(s) 852
PciSI GCTCTTC 1 cut(s) 387
PctI GAATGC 2 cut(s) 478, 887
PfeI GAWTC 1 cut(s) 878
PflMI CCANNNNNTGG 1 cut(s) 439
PkrI GCNGC 4 cut(s) 138, 562, 703, 966
PpuMI RGGWCCY 1 cut(s) 648
Psp124BI GAGCTC 1 cut(s) 768
Psp5II RGGWCCY 1 cut(s) 648
Psp6I CCWGG 1 cut(s) 1021
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 1 cut(s) 1021
PspN4I GGNNCC 6 cut(s) 286, 316, 387, 650, 742, 1003
PspOMI GGGCCC 1 cut(s) 385
PspPI GGNCC 8 cut(s) 253, 315, 385, 386, 406, 430, 648, 740
PspPPI RGGWCCY 1 cut(s) 648
PstI CTGCAG 1 cut(s) 141
PsuI RGATCY 2 cut(s) 216, 363
PvuII CAGCTG 1 cut(s) 341
RsaI GTAC 1 cut(s) 1064
RsaNI GTAC 1 cut(s) 1063
RseI CAYNNNNRTG 3 cut(s) 422, 482, 1046
SacI GAGCTC 1 cut(s) 768
SapI GCTCTTC 1 cut(s) 387
SaqAI TTAA 3 cut(s) 402, 708, 1011
SatI GCNGC 4 cut(s) 137, 561, 702, 965
Sau3AI GATC 3 cut(s) 216, 363, 732
Sau96I GGNCC 8 cut(s) 253, 315, 385, 386, 406, 430, 648, 740
ScrFI CCNGG 2 cut(s) 653, 1023
SduI GDGCHC 5 cut(s) 195, 389, 399, 768, 952
SfaNI GCATC 3 cut(s) 85, 287, 1041
SfcI CTRYAG 1 cut(s) 137
SinI GGWCC 3 cut(s) 315, 430, 648
SmiMI CAYNNNNRTG 3 cut(s) 422, 482, 1046
SmlI CTYRAG 4 cut(s) 305, 401, 725, 1035
SmoI CTYRAG 4 cut(s) 305, 401, 725, 1035
Sse9I AATT 7 cut(s) 172, 330, 348, 677, 896, 958, 979
SseBI AGGCCT 1 cut(s) 852
SsiI CCGC 1 cut(s) 750
SspMI CTAG 5 cut(s) 24, 116, 180, 495, 827
SstI GAGCTC 1 cut(s) 768
StuI AGGCCT 1 cut(s) 852
StyD4I CCNGG 2 cut(s) 651, 1021
StyI CCWWGG 1 cut(s) 256
TaaI ACNGT 5 cut(s) 149, 190, 421, 689, 1045
TaqI TCGA 1 cut(s) 504
TasI AATT 7 cut(s) 172, 330, 348, 677, 896, 958, 979
TfiI GAWTC 1 cut(s) 878
Tru1I TTAA 3 cut(s) 402, 708, 1011
Tru9I TTAA 3 cut(s) 402, 708, 1011
TscAI CASTG 3 cut(s) 152, 193, 1048
TseFI GTSAC 1 cut(s) 799
TseI GCWGC 4 cut(s) 136, 560, 701, 964
Tsp45I GTSAC 1 cut(s) 799
TspDTI ATGAA 6 cut(s) 485, 670, 698, 709, 711, 845
TspGWI ACGGA 1 cut(s) 726
TspRI CASTG 3 cut(s) 152, 193, 1048
Van91I CCANNNNNTGG 1 cut(s) 439
Vha464I CTTAAG 1 cut(s) 401
VpaK11BI GGWCC 3 cut(s) 315, 430, 648
XapI RAATTY 3 cut(s) 677, 896, 979
XceI RCATGY 1 cut(s) 457
XcmI CCANNNNNNNNNTGG 2 cut(s) 440, 868
XspI CTAG 5 cut(s) 24, 116, 180, 495, 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.