Rorug07G0055000

HOTHEAD-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0010789
Physical Location & Seq
Forward (+)
3887803 .. 3890657
2855 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0055000.1

Sequence Viewer

Length: 486 bp
ATGATTATTCATGGGCTCACTTTCATTGTCTTCCTCAATGTAGTTGGTATGTTCCTCATCATTATTCATGGCCACATTGTTGCTGTTATCCTCATGATTATTCATGGTAGCATTAGGTTGCTGCTGAATCCGGAATGGCAAACCCAACAACAATTATATGGTCAATTCGGGGACCTGACTGACCACCACCACCACCACCTGACTTTGTCCTCCTCCGCCGCCAACGGCAACATCGACCGCATCCTCTTCCAAGACCTCGTCGAGATCGTCCCTCTAGTCCAGTCCCTCATCGATCCGAACGCCAGCAGTTCATTTACGCGGCGCGGTTCAGTCACCTACAGCAAGACTCCTTATCCAAGAAAGAAGCCAGAAGGTGTGCAATACGTTAAAAAGAAGGGCTGGGCATTGGTCGAGAGATTACCAAAAGATGGAAGCTTCAAAATTGGCCTTGTGGTTTTCTTTGTGACTCACCCCCAAAATCAGTGA

Protein Analysis

161

Amino Acids

18.19

Weight (kDa)

9.52

Isoelectric Point (pI)

28.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010789)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12570
fragaria_vesca FvH4_5g01380
malus_domestica MD04G1069000.v1.1
prunus_persica Prupe.5G089100_v2.0.a1
pyrus_communis pycom04g06280
rosa_chinensis RchiOBHm_Chr7g0199501
rosa_laevigata RLG00000003788
rosa_multiflora Rmu_co8367015.1_g000001 Rmu_sc0003549.1_g000002
rosa_roxburghii Rroxscaffold_3G00256670
rosa_rugosa Rorug07G0049400 Rorug07G0055000 Rorug07G0055100 Rorug07G0055200
rosa_samantha Rh7AG179700 Rh7BG176500 Rh7BG183000 Rh7CG188500 Rh7DG182400
rosa_wichuraiana Rw7G015780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 428
AccII CGCG 2 cut(s) 319, 324
AccIII TCCGGA 1 cut(s) 130
AciI CCGC 5 cut(s) 216, 219, 238, 319, 324
AclWI GGATC 1 cut(s) 287
AcoI YGGCCR 1 cut(s) 70
AfiI CCNNNNNNNGG 1 cut(s) 428
AgsI TTSAA 1 cut(s) 439
AluBI AGCT 1 cut(s) 435
AluI AGCT 1 cut(s) 435
AlwI GGATC 1 cut(s) 287
Aor13HI TCCGGA 1 cut(s) 130
AoxI GGCC 2 cut(s) 70, 445
ApeKI GCWGC 1 cut(s) 121
AspLEI GCGC 1 cut(s) 324
AspS9I GGNCC 1 cut(s) 172
AsuHPI GGTGA 2 cut(s) 325, 461
AvaII GGWCC 1 cut(s) 172
BalI TGGCCA 1 cut(s) 72
BanII GRGCYC 1 cut(s) 18
BbsI GAAGAC 1 cut(s) 22
BbvI GCAGC 1 cut(s) 108
BccI CCATC 1 cut(s) 422
BceAI ACGGC 1 cut(s) 241
BfaI CTAG 1 cut(s) 275
BfmI CTRYAG 1 cut(s) 337
BisI GCNGC 3 cut(s) 122, 219, 320
BlsI GCNGC 3 cut(s) 123, 220, 321
Bme18I GGWCC 1 cut(s) 172
BmgT120I GGNCC 1 cut(s) 172
BmiI GGNNCC 1 cut(s) 173
BmsI GCATC 1 cut(s) 249
BpiI GAAGAC 1 cut(s) 22
Bsa29I ATCGAT 1 cut(s) 291
BsaWI WCCGGW 1 cut(s) 130
Bsc4I CCNNNNNNNGG 1 cut(s) 428
Bse1I ACTGG 1 cut(s) 280
BseAI TCCGGA 1 cut(s) 130
BseCI ATCGAT 1 cut(s) 291
BseGI GGATG 1 cut(s) 240
BseLI CCNNNNNNNGG 1 cut(s) 428
BseNI ACTGG 1 cut(s) 280
BseRI GAGGAG 1 cut(s) 202
BseXI GCAGC 1 cut(s) 108
BseYI CCCAGC 1 cut(s) 399
Bsh1236I CGCG 2 cut(s) 319, 324
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 2 cut(s) 72, 447
BshVI ATCGAT 1 cut(s) 291
BsiEI CGRYCG 1 cut(s) 238
BsiSI CCGG 1 cut(s) 131
BslFI GGGAC 3 cut(s) 185, 254, 268
BslI CCNNNNNNNGG 1 cut(s) 428
BsmFI GGGAC 3 cut(s) 185, 254, 268
BsnI GGCC 2 cut(s) 72, 447
Bsp1286I GDGCHC 1 cut(s) 18
Bsp13I TCCGGA 1 cut(s) 130
Bsp143I GATC 2 cut(s) 264, 292
BspACI CCGC 5 cut(s) 216, 219, 238, 319, 324
BspANI GGCC 2 cut(s) 72, 447
BspDI ATCGAT 1 cut(s) 291
BspEI TCCGGA 1 cut(s) 130
BspFNI CGCG 2 cut(s) 319, 324
BspHI TCATGA 1 cut(s) 93
BspLI GGNNCC 1 cut(s) 173
BspPI GGATC 1 cut(s) 287
BsrI ACTGG 1 cut(s) 280
BssMI GATC 2 cut(s) 264, 292
Bst6I CTCTTC 1 cut(s) 251
BstC8I GCNNGC 1 cut(s) 304
BstF5I GGATG 1 cut(s) 240
BstFNI CGCG 2 cut(s) 319, 324
BstHHI GCGC 1 cut(s) 324
BstKTI GATC 2 cut(s) 267, 295
BstMBI GATC 2 cut(s) 264, 292
BstMCI CGRYCG 1 cut(s) 238
BstSFI CTRYAG 1 cut(s) 337
BstUI CGCG 2 cut(s) 319, 324
BstV1I GCAGC 1 cut(s) 108
BstV2I GAAGAC 1 cut(s) 22
Bsu15I ATCGAT 1 cut(s) 291
BsuRI GGCC 2 cut(s) 72, 447
BsuTUI ATCGAT 1 cut(s) 291
BtsCI GGATG 1 cut(s) 240
Cac8I GCNNGC 1 cut(s) 304
CciI TCATGA 1 cut(s) 93
CfoI GCGC 1 cut(s) 324
Cfr13I GGNCC 1 cut(s) 172
ClaI ATCGAT 1 cut(s) 291
CviAII CATG 4 cut(s) 11, 68, 94, 104
CviJI RGCY 6 cut(s) 16, 72, 367, 399, 435, 447
CviKI_1 RGCY 6 cut(s) 16, 72, 367, 399, 435, 447
DpnI GATC 2 cut(s) 266, 294
DpnII GATC 2 cut(s) 264, 292
EaeI YGGCCR 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
EciI GGCGGA 1 cut(s) 205
Eco24I GRGCYC 1 cut(s) 18
Eco47I GGWCC 1 cut(s) 172
EcoO109I RGGNCCY 1 cut(s) 172
EcoT38I GRGCYC 1 cut(s) 18
FaeI CATG 4 cut(s) 14, 71, 97, 107
FaiI YATR 7 cut(s) 12, 50, 69, 95, 105, 157, 159
FaqI GGGAC 3 cut(s) 185, 254, 268
FatI CATG 4 cut(s) 10, 67, 93, 103
Fnu4HI GCNGC 3 cut(s) 122, 219, 320
FokI GGATG 1 cut(s) 227
FriOI GRGCYC 1 cut(s) 18
Fsp4HI GCNGC 3 cut(s) 122, 219, 320
FspBI CTAG 1 cut(s) 275
GlaI GCGC 1 cut(s) 323
GluI GCNGC 3 cut(s) 122, 219, 320
GsaI CCCAGC 1 cut(s) 403
HaeIII GGCC 2 cut(s) 72, 447
HapII CCGG 1 cut(s) 131
HhaI GCGC 1 cut(s) 324
Hin1II CATG 4 cut(s) 14, 71, 97, 107
Hin6I GCGC 1 cut(s) 322
HinP1I GCGC 1 cut(s) 322
HindIII AAGCTT 1 cut(s) 433
HinfI GANTC 3 cut(s) 127, 346, 466
HpaII CCGG 1 cut(s) 131
HphI GGTGA 2 cut(s) 325, 461
Hpy188I TCNGA 1 cut(s) 297
Hpy188III TCNNGA 4 cut(s) 94, 131, 262, 412
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 2 cut(s) 365, 388
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 1 cut(s) 379
HpySE526I ACGT 1 cut(s) 384
Hsp92II CATG 4 cut(s) 14, 71, 97, 107
HspAI GCGC 1 cut(s) 322
Kpn2I TCCGGA 1 cut(s) 130
Kzo9I GATC 2 cut(s) 264, 292
LpnPI CCDG 7 cut(s) 144, 188, 212, 293, 316, 381, 385
Lsp1109I GCAGC 1 cut(s) 108
LweI GCATC 1 cut(s) 249
MaeI CTAG 1 cut(s) 275
MaeII ACGT 1 cut(s) 384
MaeIII GTNAC 2 cut(s) 331, 463
MalI GATC 2 cut(s) 266, 294
MboI GATC 2 cut(s) 264, 292
MboII GAAGA 2 cut(s) 22, 238
MhlI GDGCHC 1 cut(s) 18
MlsI TGGCCA 1 cut(s) 72
MluCI AATT 3 cut(s) 152, 164, 441
MluNI TGGCCA 1 cut(s) 72
MlyI GAGTC 2 cut(s) 340, 460
MnlI CCTC 9 cut(s) 44, 65, 101, 220, 223, 254, 266, 282, 296
Mox20I TGGCCA 1 cut(s) 72
MroI TCCGGA 1 cut(s) 130
MscI TGGCCA 1 cut(s) 72
MseI TTAA 1 cut(s) 387
Msp20I TGGCCA 1 cut(s) 72
MspI CCGG 1 cut(s) 131
MvnI CGCG 2 cut(s) 319, 324
NdeII GATC 2 cut(s) 264, 292
NlaIII CATG 4 cut(s) 14, 71, 97, 107
NlaIV GGNNCC 1 cut(s) 173
NmuCI GTSAC 2 cut(s) 331, 463
PagI TCATGA 1 cut(s) 93
PcsI WCGNNNNNNNCGW 2 cut(s) 231, 264
PfeI GAWTC 1 cut(s) 127
PflFI GACNNNGTC 2 cut(s) 205, 257
PflMI CCANNNNNTGG 1 cut(s) 428
PkrI GCNGC 3 cut(s) 123, 220, 321
PleI GAGTC 2 cut(s) 340, 460
PpsI GAGTC 2 cut(s) 340, 460
PpuMI RGGWCCY 1 cut(s) 172
Psp5II RGGWCCY 1 cut(s) 172
PspFI CCCAGC 1 cut(s) 399
PspN4I GGNNCC 1 cut(s) 173
PspPI GGNCC 1 cut(s) 172
PspPPI RGGWCCY 1 cut(s) 172
PsyI GACNNNGTC 2 cut(s) 205, 257
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 3 cut(s) 122, 219, 320
Sau3AI GATC 2 cut(s) 264, 292
Sau96I GGNCC 1 cut(s) 172
SchI GAGTC 2 cut(s) 340, 460
SduI GDGCHC 1 cut(s) 18
SetI ASST 8 cut(s) 119, 177, 201, 258, 338, 376, 387, 437
SfaNI GCATC 1 cut(s) 249
SfcI CTRYAG 1 cut(s) 337
SinI GGWCC 1 cut(s) 172
Sse9I AATT 3 cut(s) 152, 164, 441
SsiI CCGC 5 cut(s) 216, 219, 238, 319, 324
SspMI CTAG 1 cut(s) 275
TaiI ACGT 1 cut(s) 387
TaqI TCGA 4 cut(s) 234, 261, 291, 411
TasI AATT 3 cut(s) 152, 164, 441
TauI GCSGC 2 cut(s) 221, 322
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TseFI GTSAC 2 cut(s) 331, 463
TseI GCWGC 1 cut(s) 121
Tsp45I GTSAC 2 cut(s) 331, 463
TspDTI ATGAA 4 cut(s) 13, 56, 92, 300
Tth111I GACNNNGTC 2 cut(s) 205, 257
Van91I CCANNNNNTGG 1 cut(s) 428
VpaK11BI GGWCC 1 cut(s) 172
XspI CTAG 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.