Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0010125

Physical Location & Seq

Genomic Coordinates Forward (+)

9471788 .. 9476385

Gene Structure 4598 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1069200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1398 bp

ATGGCCAAGAACGTCGGAATTCTCGCCGTCGACATCTACTTCCCCCCTACCTGTATTCAGCAGGAAGCCTTGGAGGCTCATGATGGTGCCAGCAAGGGGAAGTACACAATCGGACTTGGACAAGATTGCATGGCGTTTTGTTCAGAGGTGGAGGATGTCATCTCAATGAGTTTGACAGTGGTTACCTCCCTTCTTGAGAAATATGGGATTGATCCAAAACAAATTGGTCGCCTGGAAGTAGGCAGTGAGACTGTGATCGACAAAAGCAAATCTATTAAGACCTTCCTGATGACAATTTTTGAGAAACACGGTAACACTGACATTGAAGGTGTTGACTCAACTAATGCTTGTTATGGAGGAACTGCAGCTTTATTCAACTGTGTCAATTGGGTGGAGAGTAGCTCATGGGATGGGCGCTATGGACTTGTCGTGTGCACTGACAGCGCGGTCTACGCAGAGGGACCGGCACGACCAACTGGTGGAGCAGCTGCCATTGCCATGCTGATTGGACCTGATGCTCCTCTTGCTTTTGAGAGCAAAATTAGGGGAAGTCATATGGCTCACGTATATGATTTTTACAAGCCCGACCTTGCTAGTGAATATCCGGTTGTTGACGGAAAGCTTTCTCAGACTTGTTATCTAAAGGCTCTTGATTCTTGCTACAAGGAATTATGTAAAAAGTATGAGAAATTGGAAGGTAAACAATTTTCTATCGCTGATGCTGAGTACTTCGTATTTCATTCTCCCTATAACAAGCTCGTACAAAAAAGCTTTGCTCGTTTGGTGTTCAATGACTCCGTAAGGAATGCAAGCTCCATCGATGAGGCGGGTAAAGAAAAGCTGGCTCCATTTGCTGACCTTCCTGGTGATGATAGCTACAATAGCCGGGATCTTGAAAAGGCATCCCAGCAAGTCGCTAAGCCCCTATACGACGCTAAGGTTGTACCAACAACTTTGGTACCAAAGCAAGTTGGAAACATGTACACTGCATCTCTTTATGCGGCATTTGTATCCCTCCTCCACAACAAGCACAGCTCCTTGGATGGCAAGCGGGTGATACTGTTCTCTTATGGAAGTGGCTCAACTGCAACCATGTTCTCATTGCGTCTCAATGCTGGTCAACAGCCCTTTAGCTTGGAGAACATCGCAGCCGTGATGAATGTTGGAGAGAAGTTGAAGGCAAGACATGAGTTCCCTCCCGAGAAGTTTGTTGAAGTGATGAAGATCATGGAGCACAGGTATGGAGGCAAAGACTTTGTGACCAACCAGGACATTAGCCTTCTACTTCCAGGCACATACTATCTTACCGAAGTCGACTCTAAGTACCGTAGATTCTATGCCAAGAAGGATGCGGCCGCCACCACCAATGCCAACGGTGTGGTTGCCAACGGTCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004421 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006084 GO:0006139 GO:0006163 GO:0006629 GO:0006637 GO:0006644 GO:0006720 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0008654 GO:0009058 GO:0009117 GO:0009150 GO:0009240 GO:0009259 GO:0009506 GO:0009987 GO:0016740 GO:0016746 GO:0019287 GO:0019637 GO:0019693 GO:0030054 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0043603 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046490 GO:0046912 GO:0051186 GO:0055044 GO:0055086 GO:0071704 GO:0072521 GO:0090407 GO:1901135 GO:1901360 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00072 ko00280 ko00650 ko00900 ko01100 ko01110 ko01130 map00072 map00280 map00650 map00900 map01100 map01110 map01130

Pfam Domains

Protein Families

HMG_CoA_synt_C HMG_CoA_synt_N

Protein Analysis

466

Amino Acids

50.96

Weight (kDa)

5.98

Isoelectric Point (pI)

32.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMG_CoA_synt_N	PF01154	2 - 174	7.9e-81	Hydroxymethylglutaryl-coenzyme A synthase N terminal
HMG_CoA_synt_C	PF08540	176 - 449	3.9e-111	Hydroxymethylglutaryl-coenzyme A synthase C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010125)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11820 AT4G11820 AT4G11820
fragaria_vesca	FvH4_5g01350 FvH4_5g01350
malus_domestica	MD04G1069200.v1.1 MD06G1070000.v1.1
prunus_persica	Prupe.5G088900_v2.0.a1 Prupe.5G088900_v2.0.a1
pyrus_communis	pycom04g06300 pycom06g07050
rosa_chinensis	RchiOBHm_Chr7g0199551
rosa_laevigata	RLG00000003786
rosa_multiflora	Rmu_sc0007070.1_g000002
rosa_roxburghii	Rroxscaffold_3G00256620
rosa_rugosa	Rorug07G0055300
rosa_samantha	Rh7AG180000 Rh7BG183200 Rh7CG189000 Rh7DG182700
rosa_wichuraiana	Rw7G015810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	446
Acc65I	GGTACC	1 cut(s)	958
AccB1I	GGYRCC	2 cut(s)	86, 958
AccB7I	CCANNNNNTGG	1 cut(s)	479
AccI	GTMKAC	3 cut(s)	30, 450, 1314
AccII	CGCG	1 cut(s)	446
AciI	CCGC	6 cut(s)	446, 827, 1001, 1051, 1352, 1356
AclWI	GGATC	2 cut(s)	206, 897
AcoI	YGGCCR	2 cut(s)	3, 1353
AcsI	RAATTY	1 cut(s)	18
AfaI	GTAC	7 cut(s)	104, 728, 762, 945, 960, 983, 1325
AfiI	CCNNNNNNNGG	2 cut(s)	96, 479
AflIII	ACRYGT	1 cut(s)	978
AgsI	TTSAA	6 cut(s)	326, 376, 790, 896, 1177, 1214
AjnI	CCWGG	4 cut(s)	231, 862, 1266, 1288
Alw21I	GWGCWC	2 cut(s)	437, 1236
Alw26I	GTCTC	2 cut(s)	242, 1112
Alw44I	GTGCAC	1 cut(s)	433
AlwI	GGATC	2 cut(s)	206, 897
Ama87I	CYCGRG	1 cut(s)	1199
AoxI	GGCC	2 cut(s)	3, 1353
ApaLI	GTGCAC	1 cut(s)	433
ApeKI	GCWGC	4 cut(s)	365, 485, 488, 1148
ApoI	RAATTY	1 cut(s)	18
Asp700I	GAANNNNTTC	1 cut(s)	622
Asp718I	GGTACC	1 cut(s)	958
AspLEI	GCGC	2 cut(s)	417, 446
AspS9I	GGNCC	2 cut(s)	461, 509
AsuC2I	CCSGG	1 cut(s)	887
AsuHPI	GGTGA	2 cut(s)	878, 1066
AvaI	CYCGRG	1 cut(s)	1199
AvaII	GGWCC	2 cut(s)	461, 509
BaeGI	GKGCMC	1 cut(s)	437
BaeI	ACNNNNGTAYC	2 cut(s)	942, 975
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	2 cut(s)	86, 958
Bbv12I	GWGCWC	2 cut(s)	437, 1236
BbvI	GCAGC	4 cut(s)	377, 475, 497, 1160
BccI	CCATC	4 cut(s)	77, 404, 824, 1037
BceAI	ACGGC	2 cut(s)	11, 1136
BciT130I	CCWGG	4 cut(s)	233, 864, 1268, 1290
BciVI	GTATCC	1 cut(s)	1021
BcnI	CCSGG	1 cut(s)	887
BcoDI	GTCTC	2 cut(s)	242, 1112
BfaI	CTAG	1 cut(s)	594
BfmI	CTRYAG	1 cut(s)	363
BfoI	RGCGCY	1 cut(s)	418
BfuI	GTATCC	1 cut(s)	1021
BglI	GCCNNNNNGGC	1 cut(s)	74
BisI	GCNGC	7 cut(s)	366, 486, 489, 1002, 1149, 1353, 1356
BlpI	GCTNAGC	1 cut(s)	918
BlsI	GCNGC	7 cut(s)	367, 487, 490, 1003, 1150, 1354, 1357
BmcAI	AGTACT	1 cut(s)	728
Bme1390I	CCNGG	5 cut(s)	233, 864, 887, 1268, 1290
Bme18I	GGWCC	2 cut(s)	461, 509
BmeT110I	CYCGRG	1 cut(s)	1199
BmgT120I	GGNCC	2 cut(s)	461, 509
BmiI	GGNNCC	4 cut(s)	88, 462, 846, 960
BmrFI	CCNGG	5 cut(s)	233, 864, 887, 1268, 1290
BmsI	GCATC	5 cut(s)	505, 709, 911, 998, 1339
BplI	GAGNNNNNCTC	2 cut(s)	386, 418
Bpu10I	CCTNAGC	1 cut(s)	936
Bpu1102I	GCTNAGC	1 cut(s)	918
BpuEI	CTTGAG	1 cut(s)	215
BpuMI	CCSGG	1 cut(s)	887
Bsa29I	ATCGAT	1 cut(s)	819
BsaAI	YACGTR	1 cut(s)	565
BsaBI	GATNNNNATC	1 cut(s)	1223
BsaJI	CCNNGG	2 cut(s)	69, 1038
BsaWI	WCCGGW	1 cut(s)	604
BsaXI	ACNNNNNCTCC	2 cut(s)	1223, 1253
Bsc4I	CCNNNNNNNGG	2 cut(s)	96, 479
Bse118I	RCCGGY	1 cut(s)	463
Bse1I	ACTGG	1 cut(s)	481
Bse3DI	GCAATG	2 cut(s)	492, 1100
Bse8I	GATNNNNATC	1 cut(s)	1223
BseBI	CCWGG	4 cut(s)	233, 864, 1268, 1290
BseCI	ATCGAT	1 cut(s)	819
BseDI	CCNNGG	2 cut(s)	69, 1038
BseGI	GGATG	5 cut(s)	160, 415, 902, 1048, 1354
BseJI	GATNNNNATC	1 cut(s)	1223
BseLI	CCNNNNNNNGG	2 cut(s)	96, 479
BseMI	GCAATG	2 cut(s)	492, 1100
BseMII	CTCAG	2 cut(s)	641, 714
BseNI	ACTGG	1 cut(s)	481
BseRI	GAGGAG	2 cut(s)	510, 1007
BseSI	GKGCMC	1 cut(s)	437
BseX3I	CGGCCG	1 cut(s)	1353
BseXI	GCAGC	4 cut(s)	377, 475, 497, 1160
BseYI	CCCAGC	1 cut(s)	906
Bsh1236I	CGCG	1 cut(s)	446
Bsh1285I	CGRYCG	1 cut(s)	1356
BshFI	GGCC	2 cut(s)	5, 1355
BshNI	GGYRCC	2 cut(s)	86, 958
BshVI	ATCGAT	1 cut(s)	819
BsiEI	CGRYCG	1 cut(s)	1356
BsiHKAI	GWGCWC	2 cut(s)	437, 1236
BsiHKCI	CYCGRG	1 cut(s)	1199
BsiSI	CCGG	3 cut(s)	464, 605, 886
BslFI	GGGAC	1 cut(s)	474
BslI	CCNNNNNNNGG	2 cut(s)	96, 479
BsmAI	GTCTC	2 cut(s)	242, 1112
BsmBI	CGTCTC	1 cut(s)	1112
BsmFI	GGGAC	1 cut(s)	474
BsmI	GAATGC	1 cut(s)	811
BsnI	GGCC	2 cut(s)	5, 1355
BsoBI	CYCGRG	1 cut(s)	1199
Bsp1286I	GDGCHC	2 cut(s)	437, 1236
Bsp1407I	TGTACA	1 cut(s)	981
Bsp143I	GATC	4 cut(s)	211, 255, 889, 1224
Bsp1720I	GCTNAGC	1 cut(s)	918
BspACI	CCGC	6 cut(s)	446, 827, 1001, 1051, 1352, 1356
BspANI	GGCC	2 cut(s)	5, 1355
BspCNI	CTCAG	2 cut(s)	640, 715
BspDI	ATCGAT	1 cut(s)	819
BspFNI	CGCG	1 cut(s)	446
BspHI	TCATGA	1 cut(s)	79
BspLI	GGNNCC	4 cut(s)	88, 462, 846, 960
BspMAI	CTGCAG	1 cut(s)	367
BspPI	GGATC	2 cut(s)	206, 897
BspT107I	GGYRCC	2 cut(s)	86, 958
BsrDI	GCAATG	2 cut(s)	492, 1100
BsrFI	RCCGGY	1 cut(s)	463
BsrGI	TGTACA	1 cut(s)	981
BsrI	ACTGG	1 cut(s)	481
BssAI	RCCGGY	1 cut(s)	463
BssECI	CCNNGG	2 cut(s)	69, 1038
BssMI	GATC	4 cut(s)	211, 255, 889, 1224
BssT1I	CCWWGG	2 cut(s)	69, 1038
Bst2UI	CCWGG	4 cut(s)	233, 864, 1268, 1290
Bst4CI	ACNGT	8 cut(s)	178, 253, 311, 380, 1062, 1328, 1376, 1391
BstAUI	TGTACA	1 cut(s)	981
BstBAI	YACGTR	1 cut(s)	565
BstC8I	GCNNGC	4 cut(s)	91, 811, 843, 1049
BstDEI	CTNAG	5 cut(s)	627, 723, 918, 936, 1320
BstEII	GGTNACC	1 cut(s)	181
BstF5I	GGATG	5 cut(s)	160, 415, 902, 1048, 1354
BstFNI	CGCG	1 cut(s)	446
BstH2I	RGCGCY	1 cut(s)	418
BstHHI	GCGC	2 cut(s)	417, 446
BstKTI	GATC	4 cut(s)	214, 258, 892, 1227
BstMAI	GTCTC	2 cut(s)	242, 1112
BstMBI	GATC	4 cut(s)	211, 255, 889, 1224
BstMCI	CGRYCG	1 cut(s)	1356
BstMWI	GCNNNNNNNGC	7 cut(s)	74, 441, 452, 494, 524, 851, 882
BstNI	CCWGG	4 cut(s)	233, 864, 1268, 1290
BstNSI	RCATGY	1 cut(s)	982
BstPI	GGTNACC	1 cut(s)	181
BstSCI	CCNGG	5 cut(s)	231, 862, 885, 1266, 1288
BstSFI	CTRYAG	1 cut(s)	363
BstSLI	GKGCMC	1 cut(s)	437
BstUI	CGCG	1 cut(s)	446
BstV1I	GCAGC	4 cut(s)	377, 475, 497, 1160
BstX2I	RGATCY	1 cut(s)	889
BstXI	CCANNNNNNTGG	1 cut(s)	1378
BstYI	RGATCY	1 cut(s)	889
BstZI	CGGCCG	1 cut(s)	1353
Bsu15I	ATCGAT	1 cut(s)	819
BsuI	GTATCC	1 cut(s)	1021
BsuRI	GGCC	2 cut(s)	5, 1355
BsuTUI	ATCGAT	1 cut(s)	819
BtgZI	GCGATG	1 cut(s)	1129
BtsCI	GGATG	5 cut(s)	160, 415, 902, 1048, 1354
BtsI	GCAGTG	2 cut(s)	250, 984
BtsIMutI	CAGTG	6 cut(s)	183, 250, 315, 435, 984, 1393
Cac8I	GCNNGC	4 cut(s)	91, 811, 843, 1049
CciI	TCATGA	1 cut(s)	79
CciNI	GCGGCCGC	1 cut(s)	1353
CfoI	GCGC	2 cut(s)	417, 446
Cfr10I	RCCGGY	1 cut(s)	463
Cfr13I	GGNCC	2 cut(s)	461, 509
ClaI	ATCGAT	1 cut(s)	819
CseI	GACGC	2 cut(s)	941, 1094
Csp6I	GTAC	7 cut(s)	103, 727, 761, 944, 959, 982, 1324
CviAII	CATG	8 cut(s)	80, 130, 405, 499, 979, 1093, 1187, 1228
CviQI	GTAC	7 cut(s)	103, 727, 761, 944, 959, 982, 1324
DdeI	CTNAG	5 cut(s)	627, 723, 918, 936, 1320
DpnI	GATC	4 cut(s)	213, 257, 891, 1226
DpnII	GATC	4 cut(s)	211, 255, 889, 1224
DrdI	GACNNNNNNGTC	1 cut(s)	446
DseDI	GACNNNNNNGTC	1 cut(s)	446
EaeI	YGGCCR	2 cut(s)	3, 1353
EagI	CGGCCG	1 cut(s)	1353
EclXI	CGGCCG	1 cut(s)	1353
Eco130I	CCWWGG	2 cut(s)	69, 1038
Eco47I	GGWCC	2 cut(s)	461, 509
Eco52I	CGGCCG	1 cut(s)	1353
Eco88I	CYCGRG	1 cut(s)	1199
Eco91I	GGTNACC	1 cut(s)	181
EcoO65I	GGTNACC	1 cut(s)	181
EcoRI	GAATTC	1 cut(s)	18
EcoRII	CCWGG	4 cut(s)	231, 862, 1266, 1288
EcoT14I	CCWWGG	2 cut(s)	69, 1038
ErhI	CCWWGG	2 cut(s)	69, 1038
Esp3I	CGTCTC	1 cut(s)	1112
FaeI	CATG	8 cut(s)	83, 133, 408, 502, 982, 1096, 1190, 1231
FaqI	GGGAC	1 cut(s)	474
FatI	CATG	8 cut(s)	79, 129, 404, 498, 978, 1092, 1186, 1227
FauI	CCCGC	2 cut(s)	820, 1044
FauNDI	CATATG	1 cut(s)	555
FblI	GTMKAC	3 cut(s)	30, 450, 1314
Fnu4HI	GCNGC	7 cut(s)	366, 486, 489, 1002, 1149, 1353, 1356
FokI	GGATG	5 cut(s)	167, 422, 889, 1055, 1361
Fsp4HI	GCNGC	7 cut(s)	366, 486, 489, 1002, 1149, 1353, 1356
FspBI	CTAG	1 cut(s)	594
GlaI	GCGC	2 cut(s)	416, 445
GluI	GCNGC	7 cut(s)	366, 486, 489, 1002, 1149, 1353, 1356
GsaI	CCCAGC	1 cut(s)	910
HaeII	RGCGCY	1 cut(s)	418
HaeIII	GGCC	2 cut(s)	5, 1355
HapII	CCGG	3 cut(s)	464, 605, 886
HgaI	GACGC	2 cut(s)	941, 1094
HhaI	GCGC	2 cut(s)	417, 446
Hin1II	CATG	8 cut(s)	83, 133, 408, 502, 982, 1096, 1190, 1231
Hin6I	GCGC	2 cut(s)	415, 444
HinP1I	GCGC	2 cut(s)	415, 444
HincII	GTYRAC	5 cut(s)	31, 334, 613, 1121, 1315
HindII	GTYRAC	5 cut(s)	31, 334, 613, 1121, 1315
HindIII	AAGCTT	2 cut(s)	620, 769
HinfI	GANTC	5 cut(s)	335, 653, 794, 1316, 1332
HpaII	CCGG	3 cut(s)	464, 605, 886
HphI	GGTGA	2 cut(s)	878, 1066
Hpy188I	TCNGA	4 cut(s)	17, 113, 145, 630
Hpy188III	TCNNGA	6 cut(s)	80, 194, 286, 650, 893, 1199
Hpy99I	CGWCG	3 cut(s)	17, 32, 935
HpyAV	CCTTC	8 cut(s)	200, 292, 320, 689, 869, 1171, 1289, 1339
HpyCH4III	ACNGT	8 cut(s)	178, 253, 311, 380, 1062, 1328, 1376, 1391
HpyCH4IV	ACGT	2 cut(s)	12, 564
HpyCH4V	TGCA	6 cut(s)	129, 365, 435, 809, 989, 1088
HpyF10VI	GCNNNNNNNGC	7 cut(s)	74, 441, 452, 494, 524, 851, 882
HpyF3I	CTNAG	5 cut(s)	627, 723, 918, 936, 1320
HpySE526I	ACGT	2 cut(s)	12, 564
Hsp92II	CATG	8 cut(s)	83, 133, 408, 502, 982, 1096, 1190, 1231
HspAI	GCGC	2 cut(s)	415, 444
KpnI	GGTACC	1 cut(s)	962
Kzo9I	GATC	4 cut(s)	211, 255, 889, 1224
LmnI	GCTCC	6 cut(s)	482, 523, 818, 850, 1040, 1231
Lsp1109I	GCAGC	4 cut(s)	377, 475, 497, 1160
LweI	GCATC	5 cut(s)	505, 709, 911, 998, 1339
MaeI	CTAG	1 cut(s)	594
MaeII	ACGT	2 cut(s)	12, 564
MaeIII	GTNAC	4 cut(s)	181, 311, 1258, 1391
MalI	GATC	4 cut(s)	213, 257, 891, 1226
MboI	GATC	4 cut(s)	211, 255, 889, 1224
MboII	GAAGA	1 cut(s)	1234
MfeI	CAATTG	1 cut(s)	385
MflI	RGATCY	1 cut(s)	889
MhlI	GDGCHC	2 cut(s)	437, 1236
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	8 cut(s)	18, 222, 294, 385, 540, 668, 689, 704
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	3 cut(s)	329, 788, 1310
MmeI	TCCRAC	2 cut(s)	952, 1144
Mox20I	TGGCCA	1 cut(s)	5
MroXI	GAANNNNTTC	1 cut(s)	622
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	1 cut(s)	276
MslI	CAYNNNNRTG	5 cut(s)	84, 164, 497, 567, 1239
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	488
MspI	CCGG	3 cut(s)	464, 605, 886
MspR9I	CCNGG	5 cut(s)	233, 864, 887, 1268, 1290
MunI	CAATTG	1 cut(s)	385
Mva1269I	GAATGC	1 cut(s)	811
MvaI	CCWGG	4 cut(s)	233, 864, 1268, 1290
MvnI	CGCG	1 cut(s)	446
MwoI	GCNNNNNNNGC	7 cut(s)	74, 441, 452, 494, 524, 851, 882
NciI	CCSGG	1 cut(s)	887
NdeI	CATATG	1 cut(s)	555
NdeII	GATC	4 cut(s)	211, 255, 889, 1224
NlaIII	CATG	8 cut(s)	83, 133, 408, 502, 982, 1096, 1190, 1231
NlaIV	GGNNCC	4 cut(s)	88, 462, 846, 960
NmuCI	GTSAC	2 cut(s)	1258, 1391
NotI	GCGGCCGC	1 cut(s)	1353
NspI	RCATGY	1 cut(s)	982
PagI	TCATGA	1 cut(s)	79
PciI	ACATGT	1 cut(s)	978
PctI	GAATGC	1 cut(s)	811
PdmI	GAANNNNTTC	1 cut(s)	622
PfeI	GAWTC	2 cut(s)	653, 1332
PflMI	CCANNNNNTGG	1 cut(s)	479
PkrI	GCNGC	7 cut(s)	367, 487, 490, 1003, 1150, 1354, 1357
PleI	GAGTC	3 cut(s)	329, 788, 1310
PpsI	GAGTC	3 cut(s)	329, 788, 1310
Ppu21I	YACGTR	1 cut(s)	565
PscI	ACATGT	1 cut(s)	978
Psp6I	CCWGG	4 cut(s)	231, 862, 1266, 1288
PspEI	GGTNACC	1 cut(s)	181
PspFI	CCCAGC	1 cut(s)	906
PspGI	CCWGG	4 cut(s)	231, 862, 1266, 1288
PspN4I	GGNNCC	4 cut(s)	88, 462, 846, 960
PspPI	GGNCC	2 cut(s)	461, 509
PstI	CTGCAG	1 cut(s)	367
PsuI	RGATCY	1 cut(s)	889
PvuII	CAGCTG	1 cut(s)	488
RsaI	GTAC	7 cut(s)	104, 728, 762, 945, 960, 983, 1325
RsaNI	GTAC	7 cut(s)	103, 727, 761, 944, 959, 982, 1324
RseI	CAYNNNNRTG	5 cut(s)	84, 164, 497, 567, 1239
SalI	GTCGAC	2 cut(s)	29, 1313
SaqAI	TTAA	1 cut(s)	276
SatI	GCNGC	7 cut(s)	366, 486, 489, 1002, 1149, 1353, 1356
Sau3AI	GATC	4 cut(s)	211, 255, 889, 1224
Sau96I	GGNCC	2 cut(s)	461, 509
ScaI	AGTACT	1 cut(s)	728
SchI	GAGTC	3 cut(s)	329, 788, 1310
ScrFI	CCNGG	5 cut(s)	233, 864, 887, 1268, 1290
SduI	GDGCHC	2 cut(s)	437, 1236
SfaNI	GCATC	5 cut(s)	505, 709, 911, 998, 1339
SfcI	CTRYAG	1 cut(s)	363
SinI	GGWCC	2 cut(s)	461, 509
SmiMI	CAYNNNNRTG	5 cut(s)	84, 164, 497, 567, 1239
SmlI	CTYRAG	1 cut(s)	194
SmoI	CTYRAG	1 cut(s)	194
Sse9I	AATT	8 cut(s)	18, 222, 294, 385, 540, 668, 689, 704
SsiI	CCGC	6 cut(s)	446, 827, 1001, 1051, 1352, 1356
SspMI	CTAG	1 cut(s)	594
StyD4I	CCNGG	5 cut(s)	231, 862, 885, 1266, 1288
StyI	CCWWGG	2 cut(s)	69, 1038
TaaI	ACNGT	8 cut(s)	178, 253, 311, 380, 1062, 1328, 1376, 1391
TaiI	ACGT	2 cut(s)	15, 567
TaqI	TCGA	4 cut(s)	30, 258, 819, 1314
TasI	AATT	8 cut(s)	18, 222, 294, 385, 540, 668, 689, 704
TatI	WGTACW	3 cut(s)	102, 726, 981
TauI	GCSGC	3 cut(s)	1004, 1355, 1358
TfiI	GAWTC	2 cut(s)	653, 1332
Tru1I	TTAA	1 cut(s)	276
Tru9I	TTAA	1 cut(s)	276
TscAI	CASTG	5 cut(s)	183, 250, 322, 442, 991
TseFI	GTSAC	2 cut(s)	1258, 1391
TseI	GCWGC	4 cut(s)	365, 485, 488, 1148
Tsp45I	GTSAC	2 cut(s)	1258, 1391
TspDTI	ATGAA	3 cut(s)	728, 1172, 1235
TspGWI	ACGGA	2 cut(s)	630, 787
TspRI	CASTG	5 cut(s)	183, 250, 322, 442, 991
Van91I	CCANNNNNTGG	1 cut(s)	479
VneI	GTGCAC	1 cut(s)	433
VpaK11BI	GGWCC	2 cut(s)	461, 509
XapI	RAATTY	1 cut(s)	18
XceI	RCATGY	1 cut(s)	982
XmiI	GTMKAC	3 cut(s)	30, 450, 1314
XmnI	GAANNNNTTC	1 cut(s)	622
XspI	CTAG	1 cut(s)	594
ZrmI	AGTACT	1 cut(s)	728

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.