Rorug07G0055300
TCP Family

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0005124
Physical Location & Seq
Reverse (-)
3909706 .. 3913572
3867 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0055300.1

Sequence Viewer

Length: 1038 bp
ATGCCGGCTCTTCATACTGCCTCGGTTGATTTTGGTGCTGTCCAGGAAACAAAAGGCTCGTCAAAGAGTTTCCCAAAGCATGAGACACGCCATCTTTCAGAGCACAAGAATATTGCCAGATGTGCAAGGTTTAGTCCGGATGGGAAGTTTGTTGCGACTGGAAGTTCAGACACGTCAATAAAGCTATTTGAGGTGGCAAAAGTTAAGCAAATGATGCTTCCAGAGGCAAGAGATGGTCCTGTGCGACCTGTCATACGTACATTCTATGACCACTTACAACCCATAAATGATTTGGATTTCCATCCTCAGAGCTCTATTCTTATATCTGGAGCAAAAGATAACACAATAAAGTTCTTTGATTATTCCAAAATGACTGCAAAGAGAGCATTCAGAGTTATCCAGGACACACACAATGTGCGTTCAGTAGCGTTTCATCCTTCTGGGGAGTTTCTTTTAGCTGGGACTGATCATCCAATTCCACACTTATATGATATCAATACCTTCCAGTGTTACCTATCTGCAAATGCCCCAGAAATTGATGTTAATGGAGCAATAAATCAGGTCAGGTATTCAGCTACCGGTGGCATGTATGTTACAGCTTCTAAAGATGGTGCTATTCGCTTATGGGATGGTGTTACTGCCAGTTGTGTACGTTCCGTAGTTGGTGCACATGGCACAGCAGAGGCAACCAGTGCAAATTTTACCAAGGACGAAAGGTTTGTTCTCTCTTCTGGAAAGGATTCTACTGTGAAGCTTTGGGAAGTTGGCACTGGAAAATTGGTTAAACAATATTTAGGAGCTACACATACACAATTAAGATGTCAGGCTGTTTTTAATAATACGGAGGAATTTGTACTGTCCATGGATGAACCAAGCAATGAGATTGTTATCTGGGATGCTCTGACAGCAGAAGTAGTAGCAAGGTGGCCATCCAATCATATTGGTGCTTTCCGCTGGCTCGAGCACTCACCAACTGAAGCCGCATTTGTTTCCTGTGGGACTGACAGATCAATACGGTTCTGGAAGGAAGGCGTGTAG

Protein Analysis

345

Amino Acids

38.17

Weight (kDa)

6.92

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 11 - 210 2e-12 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 28 - 82 4.4e-06 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 28 - 123 9e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 28 - 173 2e-14 WDR3 first beta-propeller domain
WD40 PF00400 29 - 63 1e-06 WD domain, G-beta repeat
WD40_Gbeta PF25391 29 - 218 5.4e-12 G protein beta WD-40 repeat protein
Beta-prop_CAF1B_HIR1 PF24105 31 - 173 6.2e-12 CAF1B/HIR1 beta-propeller domain
NBCH_WD40 PF20426 32 - 257 2.7e-09 Neurobeachin beta propeller domain
Beta-prop_WDR5 PF25175 32 - 342 1.2e-51 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 34 - 220 5.4e-17 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 81 - 152 6.1e-08 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 84 - 225 2.4e-18 Prp19 WD40 domain
WD40 PF00400 84 - 120 2.3e-07 WD domain, G-beta repeat
WD40_MABP1-WDR62_2nd PF24782 85 - 254 1.4e-06 MABP1/WDR62 second WD40 domain
Beta-prop_THOC3 PF25174 87 - 312 2.3e-26 THOC3 beta-propeller domain
EIF3I PF24805 87 - 277 4.5e-14 EIF3I
WDR55 PF24796 93 - 291 7.9e-15 WDR55
WD40_CDC20-Fz PF24807 97 - 341 1.9e-19 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 98 - 263 4.9e-15 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 104 - 341 6.9e-11 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 174 - 267 7e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 183 - 276 6.7e-13 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 230 - 343 1.3e-08 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 235 - 341 2.9e-07 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 136
AciI CCGC 2 cut(s) 952, 981
AcoI YGGCCR 1 cut(s) 926
AcsI RAATTY 2 cut(s) 697, 848
AcuI CTGAAG 1 cut(s) 996
AdeI CACNNNGTG 1 cut(s) 415
AfaI GTAC 3 cut(s) 259, 651, 855
AflIII ACRYGT 1 cut(s) 171
AgeI ACCGGT 1 cut(s) 578
AjiI CACGTC 1 cut(s) 174
AjnI CCWGG 2 cut(s) 42, 399
AluBI AGCT 7 cut(s) 184, 312, 458, 575, 599, 754, 800
AluI AGCT 7 cut(s) 184, 312, 458, 575, 599, 754, 800
Alw21I GWGCWC 4 cut(s) 105, 314, 670, 966
Alw26I GTCTC 1 cut(s) 77
Alw44I GTGCAC 1 cut(s) 666
Ama87I CYCGRG 1 cut(s) 959
Aor13HI TCCGGA 1 cut(s) 136
AoxI GGCC 1 cut(s) 926
ApaLI GTGCAC 1 cut(s) 666
ApoI RAATTY 2 cut(s) 697, 848
ArsI GACNNNNNNTTYG 4 cut(s) 44, 76, 701, 733
AsiGI ACCGGT 1 cut(s) 578
Asp700I GAANNNNTTC 1 cut(s) 739
AspS9I GGNCC 1 cut(s) 236
AsuHPI GGTGA 1 cut(s) 960
AvaI CYCGRG 1 cut(s) 959
AvaII GGWCC 1 cut(s) 236
BaeGI GKGCMC 1 cut(s) 670
BalI TGGCCA 1 cut(s) 928
BanII GRGCYC 1 cut(s) 314
Bbv12I GWGCWC 4 cut(s) 105, 314, 670, 966
BccI CCATC 7 cut(s) 99, 134, 227, 309, 602, 623, 937
BciT130I CCWGG 2 cut(s) 44, 401
BclI TGATCA 1 cut(s) 466
BcoDI GTCTC 1 cut(s) 77
BisI GCNGC 1 cut(s) 981
BlsI GCNGC 1 cut(s) 982
Bme1390I CCNGG 2 cut(s) 44, 401
Bme18I GGWCC 1 cut(s) 236
BmeT110I CYCGRG 1 cut(s) 959
BmgBI CACGTC 1 cut(s) 174
BmgT120I GGNCC 1 cut(s) 236
BmrFI CCNGG 2 cut(s) 44, 401
BmsI GCATC 2 cut(s) 204, 886
BpmI CTGGAG 1 cut(s) 348
BsaAI YACGTR 1 cut(s) 257
BsaBI GATNNNNATC 2 cut(s) 300, 887
BsaJI CCNNGG 3 cut(s) 21, 705, 861
BsaWI WCCGGW 2 cut(s) 136, 578
Bse118I RCCGGY 2 cut(s) 4, 578
Bse1I ACTGG 5 cut(s) 163, 505, 642, 690, 775
Bse3DI GCAATG 1 cut(s) 883
Bse8I GATNNNNATC 2 cut(s) 300, 887
BseAI TCCGGA 1 cut(s) 136
BseBI CCWGG 2 cut(s) 44, 401
BseDI CCNNGG 3 cut(s) 21, 705, 861
BseGI GGATG 8 cut(s) 145, 301, 433, 469, 634, 871, 901, 929
BseJI GATNNNNATC 2 cut(s) 300, 887
BseMI GCAATG 1 cut(s) 883
BseMII CTCAG 1 cut(s) 320
BseNI ACTGG 5 cut(s) 163, 505, 642, 690, 775
BseSI GKGCMC 1 cut(s) 670
BseYI CCCAGC 1 cut(s) 458
BshFI GGCC 1 cut(s) 928
BshTI ACCGGT 1 cut(s) 578
BsiHKAI GWGCWC 4 cut(s) 105, 314, 670, 966
BsiHKCI CYCGRG 1 cut(s) 959
BsiSI CCGG 3 cut(s) 5, 137, 579
BslFI GGGAC 2 cut(s) 475, 1012
BsmAI GTCTC 1 cut(s) 77
BsmFI GGGAC 2 cut(s) 475, 1012
BsmI GAATGC 1 cut(s) 386
BsnI GGCC 1 cut(s) 928
BsoBI CYCGRG 1 cut(s) 959
Bsp1286I GDGCHC 4 cut(s) 105, 314, 670, 966
Bsp13I TCCGGA 1 cut(s) 136
Bsp143I GATC 2 cut(s) 466, 1007
Bsp19I CCATGG 1 cut(s) 861
BspACI CCGC 2 cut(s) 952, 981
BspANI GGCC 1 cut(s) 928
BspCNI CTCAG 1 cut(s) 319
BspEI TCCGGA 1 cut(s) 136
BspQI GCTCTTC 1 cut(s) 15
BsrDI GCAATG 1 cut(s) 883
BsrFI RCCGGY 2 cut(s) 4, 578
BsrI ACTGG 5 cut(s) 163, 505, 642, 690, 775
BssAI RCCGGY 2 cut(s) 4, 578
BssECI CCNNGG 3 cut(s) 21, 705, 861
BssMI GATC 2 cut(s) 466, 1007
BssT1I CCWWGG 2 cut(s) 705, 861
Bst2UI CCWGG 2 cut(s) 44, 401
Bst4CI ACNGT 3 cut(s) 748, 858, 1017
Bst6I CTCTTC 2 cut(s) 15, 733
BstAPI GCANNNNNTGC 2 cut(s) 214, 692
BstBAI YACGTR 1 cut(s) 257
BstC8I GCNNGC 2 cut(s) 6, 956
BstDEI CTNAG 1 cut(s) 306
BstDSI CCRYGG 1 cut(s) 861
BstF5I GGATG 8 cut(s) 145, 301, 433, 469, 634, 871, 901, 929
BstKTI GATC 2 cut(s) 469, 1010
BstMAI GTCTC 1 cut(s) 77
BstMBI GATC 2 cut(s) 466, 1007
BstMWI GCNNNNNNNGC 5 cut(s) 122, 214, 383, 692, 905
BstNI CCWGG 2 cut(s) 44, 401
BstNSI RCATGY 1 cut(s) 589
BstSCI CCNGG 2 cut(s) 42, 399
BstSLI GKGCMC 1 cut(s) 670
BstSNI TACGTA 1 cut(s) 257
BsuRI GGCC 1 cut(s) 928
BtgI CCRYGG 1 cut(s) 861
BtrI CACGTC 1 cut(s) 174
BtsCI GGATG 8 cut(s) 145, 301, 433, 469, 634, 871, 901, 929
BtsIMutI CAGTG 3 cut(s) 512, 697, 768
Cac8I GCNNGC 2 cut(s) 6, 956
Cfr10I RCCGGY 2 cut(s) 4, 578
Cfr13I GGNCC 1 cut(s) 236
Csp6I GTAC 3 cut(s) 258, 650, 854
CspAI ACCGGT 1 cut(s) 578
CviAII CATG 4 cut(s) 80, 586, 671, 862
CviQI GTAC 3 cut(s) 258, 650, 854
DdeI CTNAG 1 cut(s) 306
DpnI GATC 2 cut(s) 468, 1009
DpnII GATC 2 cut(s) 466, 1007
DraIII CACNNNGTG 1 cut(s) 415
EaeI YGGCCR 1 cut(s) 926
Eam1104I CTCTTC 2 cut(s) 15, 733
EarI CTCTTC 2 cut(s) 15, 733
Ecl136II GAGCTC 1 cut(s) 312
Eco105I TACGTA 1 cut(s) 257
Eco130I CCWWGG 2 cut(s) 705, 861
Eco24I GRGCYC 1 cut(s) 314
Eco32I GATATC 1 cut(s) 493
Eco47I GGWCC 1 cut(s) 236
Eco53kI GAGCTC 1 cut(s) 312
Eco57I CTGAAG 1 cut(s) 996
Eco88I CYCGRG 1 cut(s) 959
EcoICRI GAGCTC 1 cut(s) 312
EcoRII CCWGG 2 cut(s) 42, 399
EcoRV GATATC 1 cut(s) 493
EcoT14I CCWWGG 2 cut(s) 705, 861
EcoT38I GRGCYC 1 cut(s) 314
ErhI CCWWGG 2 cut(s) 705, 861
FaeI CATG 4 cut(s) 83, 589, 674, 865
FaqI GGGAC 2 cut(s) 475, 1012
FatI CATG 4 cut(s) 79, 585, 670, 861
FbaI TGATCA 1 cut(s) 466
Fnu4HI GCNGC 1 cut(s) 981
FokI GGATG 8 cut(s) 152, 288, 420, 456, 641, 878, 908, 916
FriOI GRGCYC 1 cut(s) 314
Fsp4HI GCNGC 1 cut(s) 981
GluI GCNGC 1 cut(s) 981
GsaI CCCAGC 1 cut(s) 462
GsuI CTGGAG 1 cut(s) 348
HaeIII GGCC 1 cut(s) 928
HapII CCGG 3 cut(s) 5, 137, 579
Hin1II CATG 4 cut(s) 83, 589, 674, 865
HindIII AAGCTT 1 cut(s) 752
HinfI GANTC 1 cut(s) 740
HpaII CCGG 3 cut(s) 5, 137, 579
HphI GGTGA 1 cut(s) 960
Hpy166II GTNNAC 2 cut(s) 650, 668
Hpy188I TCNGA 5 cut(s) 100, 169, 309, 392, 903
Hpy188III TCNNGA 5 cut(s) 137, 221, 327, 732, 1021
Hpy8I GTNNAC 2 cut(s) 650, 668
HpyAV CCTTC 4 cut(s) 447, 511, 1018, 1022
HpyCH4III ACNGT 3 cut(s) 748, 858, 1017
HpyCH4IV ACGT 3 cut(s) 173, 256, 652
HpyCH4V TGCA 5 cut(s) 125, 377, 521, 668, 695
HpyF10VI GCNNNNNNNGC 5 cut(s) 122, 214, 383, 692, 905
HpyF3I CTNAG 1 cut(s) 306
HpySE526I ACGT 3 cut(s) 173, 256, 652
Hsp92II CATG 4 cut(s) 83, 589, 674, 865
Kpn2I TCCGGA 1 cut(s) 136
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 1 cut(s) 466
Kzo9I GATC 2 cut(s) 466, 1007
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 3 cut(s) 329, 548, 797
LweI GCATC 2 cut(s) 204, 886
MaeII ACGT 3 cut(s) 173, 256, 652
MaeIII GTNAC 3 cut(s) 509, 592, 634
MalI GATC 2 cut(s) 468, 1009
MboI GATC 2 cut(s) 466, 1007
MboII GAAGA 1 cut(s) 720
MhlI GDGCHC 4 cut(s) 105, 314, 670, 966
MlsI TGGCCA 1 cut(s) 928
MluCI AATT 6 cut(s) 474, 534, 697, 776, 812, 848
MluNI TGGCCA 1 cut(s) 928
MnlI CCTC 6 cut(s) 31, 184, 217, 315, 676, 838
Mox20I TGGCCA 1 cut(s) 928
MroI TCCGGA 1 cut(s) 136
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 1 cut(s) 739
MscI TGGCCA 1 cut(s) 928
MseI TTAA 5 cut(s) 204, 543, 783, 815, 834
MslI CAYNNNNRTG 2 cut(s) 486, 942
Msp20I TGGCCA 1 cut(s) 928
MspA1I CMGCKG 1 cut(s) 954
MspI CCGG 3 cut(s) 5, 137, 579
MspR9I CCNGG 2 cut(s) 44, 401
Mva1269I GAATGC 1 cut(s) 386
MvaI CCWGG 2 cut(s) 44, 401
MwoI GCNNNNNNNGC 5 cut(s) 122, 214, 383, 692, 905
NaeI GCCGGC 1 cut(s) 6
NcoI CCATGG 1 cut(s) 861
NdeII GATC 2 cut(s) 466, 1007
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 4 cut(s) 83, 589, 674, 865
NspI RCATGY 1 cut(s) 589
PaeR7I CTCGAG 1 cut(s) 959
PciSI GCTCTTC 1 cut(s) 15
PctI GAATGC 1 cut(s) 386
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 739
PfeI GAWTC 1 cut(s) 740
PfoI TCCNGGA 2 cut(s) 42, 399
PinAI ACCGGT 1 cut(s) 578
PkrI GCNGC 1 cut(s) 982
Ppu21I YACGTR 1 cut(s) 257
Psp124BI GAGCTC 1 cut(s) 314
Psp6I CCWGG 2 cut(s) 42, 399
PspFI CCCAGC 1 cut(s) 458
PspGI CCWGG 2 cut(s) 42, 399
PspPI GGNCC 1 cut(s) 236
PspXI VCTCGAGB 1 cut(s) 959
RsaI GTAC 3 cut(s) 259, 651, 855
RsaNI GTAC 3 cut(s) 258, 650, 854
RseI CAYNNNNRTG 2 cut(s) 486, 942
SacI GAGCTC 1 cut(s) 314
SapI GCTCTTC 1 cut(s) 15
SaqAI TTAA 5 cut(s) 204, 543, 783, 815, 834
SatI GCNGC 1 cut(s) 981
Sau3AI GATC 2 cut(s) 466, 1007
Sau96I GGNCC 1 cut(s) 236
ScrFI CCNGG 2 cut(s) 44, 401
SduI GDGCHC 4 cut(s) 105, 314, 670, 966
SfaNI GCATC 2 cut(s) 204, 886
Sfr274I CTCGAG 1 cut(s) 959
SinI GGWCC 1 cut(s) 236
SlaI CTCGAG 1 cut(s) 959
SmiMI CAYNNNNRTG 2 cut(s) 486, 942
SmlI CTYRAG 1 cut(s) 959
SmoI CTYRAG 1 cut(s) 959
SnaBI TACGTA 1 cut(s) 257
Sse9I AATT 6 cut(s) 474, 534, 697, 776, 812, 848
SsiI CCGC 2 cut(s) 952, 981
SspI AATATT 2 cut(s) 112, 791
SstI GAGCTC 1 cut(s) 314
StyD4I CCNGG 2 cut(s) 42, 399
StyI CCWWGG 2 cut(s) 705, 861
TaaI ACNGT 3 cut(s) 748, 858, 1017
TaiI ACGT 3 cut(s) 176, 259, 655
TaqI TCGA 1 cut(s) 960
TasI AATT 6 cut(s) 474, 534, 697, 776, 812, 848
TatI WGTACW 1 cut(s) 853
TauI GCSGC 1 cut(s) 983
TfiI GAWTC 1 cut(s) 740
Tru1I TTAA 5 cut(s) 204, 543, 783, 815, 834
Tru9I TTAA 5 cut(s) 204, 543, 783, 815, 834
TscAI CASTG 3 cut(s) 512, 697, 775
TspDTI ATGAA 2 cut(s) 422, 882
TspGWI ACGGA 2 cut(s) 646, 857
TspRI CASTG 3 cut(s) 512, 697, 775
VneI GTGCAC 1 cut(s) 666
VpaK11BI GGWCC 1 cut(s) 236
XapI RAATTY 2 cut(s) 697, 848
XceI RCATGY 1 cut(s) 589
XcmI CCANNNNNNNNNTGG 1 cut(s) 289
XhoI CTCGAG 1 cut(s) 959
XmnI GAANNNNTTC 1 cut(s) 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.