Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0005124

Physical Location & Seq

Genomic Coordinates Reverse (-)

24813707 .. 24815140

Gene Structure 1434 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom07g24010.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1434 bp

ATGAGTAACAGGATTATGGAGTTTCTGAATATATCCGCAAATGATTACAGCATGGTTTTCACCGCCAACAGAACATCGGCTTTTAAACTTGTCGCGGAATCTTACCCGTTCAAGACTAGTAGGAAGCTGCTGACGGTGTATGATTATGAGAGTGAGGCAGTGGAAGGGATGATTAACAGCTCAGAAAAGAGAGGAGCCAAAGTCATGGCGGCGGAGTTTTCGTGGCCCAGGCTGAGGATTCAATCGGCAAAGCTGAGGAAGATGGTTGTTAGCAAGAGGAAGAAGAAAAAGAAAAGGGGGCTTTTTGTGTTCCCTGTACATTCTAGGATAACAGGCTCCAGATATCCTTATGTATGGATGACCATGGCGCAGGAGAACGGGTGGCATGTCTTGGTTGATGCTTGTGCATTAGGGCCAAAAGATATGGACAGCTTCGGCCTCTCACTCTTTCAACCCGATTTCCTCATTTCTTCTTTCTACAAGATTTGTGGGGAAAACCCTTCTGGCTTTGCATGCCTCTTTGTCAAGAAATCTGTTGTTTCAGTCCTAGAAATTTCCACAAGTACAGGAATTGTAAATCTTGTTCCGGCAAAGCAATTGCTTCAAATAGCAGGGGATTCTTCTGGTACCGACACAGAGCTGGAGCAGATATGTAGACTTGGCATGCAGCTAGATGCATTAGGCACGGCAAGTTCTTTCTCCGGTCTGATATCCAATCACACAACACAGGCAAGAAGGTTGAAACAAGAACGCTCTGAGAGAGCTGAAGAAGCTGCAAACTGGGAAGAAATCGAAACGTCGTCAGAGATTTGGAGTTCAGAAAAACATGCCAATTTAATGAAGGATGAGAATTCCAGAAGCAGCGGTAATGGGGACCTAGAGGTTGTATGCAGGGGGCTGGATCTGGTGGATTCACTGGGGCTAGTGATGATTACTAACAGGGCAAGGTACCTGATCAATTGGCTGGTGAGCTCATTGAGAAAGCTTAAGCATCCTAACAGCGAGGGAATTCCTCTGGTTAAAATCTATGGGCCAAAGATCAAATTCGACAGGGGACCAGCTCTGGCATTCAATGTATATGATTGGAAAGGAGAGAAGGTTGAGCCTATTCTCATACAGAAACTAGCTGACAGAAACAATATTTCTCTTAGCTATGGATTCTTACACCACATCTGGTTCTCGGATAAGTACACTGACGAGAAACGGAGAATCCTAGAGAAAAGAGAAGGCGGAGTGAAGGGAACGGGTGCTAACAAGTTCAAGGCGAAATCTGATCTTGGAATTACCGTGGTTACAGCTGCACTCGGATTCTTGACCAACTTTGAAGACGTATACAGGCTTTGGGCCTTTGTTGCACAGTTCCTGGATGCAGACTTTGTGGAGAAGGAGAGATGGAGATATACAGCTCTTAATCAGAAAACAGTTGAAGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Aminotran_5

Protein Analysis

478

Amino Acids

53.93

Weight (kDa)

9.24

Isoelectric Point (pI)

39.23

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005124)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51920
fragaria_vesca	FvH4_4g10020 FvH4_5g01360
malus_domestica	MD00G1005100.v1.1 MD04G1056600.v1.1 MD04G1069100.v1.1
prunus_persica	Prupe.2G288200_v2.0.a1 Prupe.5G089000_v2.0.a1
pyrus_communis	pycom04g06290 pycom07g24010
rosa_chinensis	RchiOBHm_Chr7g0199531
rosa_laevigata	RLG00000003787 RLG00000026609
rosa_multiflora	Rmu_sc0001198.1_g000009 Rmu_sc0005594.1_g000022 Rmu_sc0007070.1_g000003 Rmu_sc0016074.1_g000005
rosa_roxburghii	Rroxscaffold_3G00256630 Rroxscaffold_4G00282180
rosa_rugosa	Rorug07G0055300
rosa_samantha	Rh1AG406800 Rh1BG370900 Rh1CG383700 Rh1DG400600 Rh7AG179800 Rh7BG183100 Rh7CG188800 Rh7DG182500
rosa_wichuraiana	Rw1G036120 Rw7G015790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	2 cut(s)	626, 948
AccB1I	GGYRCC	2 cut(s)	626, 948
AccI	GTMKAC	2 cut(s)	655, 1332
AccII	CGCG	1 cut(s)	95
AciI	CCGC	7 cut(s)	36, 63, 95, 209, 212, 864, 1230
AclWI	GGATC	1 cut(s)	909
AcsI	RAATTY	4 cut(s)	552, 850, 1008, 1043
AcuI	CTGAAG	1 cut(s)	786
AfaI	GTAC	5 cut(s)	318, 565, 628, 950, 1190
AfiI	CCNNNNNNNGG	1 cut(s)	234
AflII	CTTAAG	1 cut(s)	986
AgsI	TTSAA	9 cut(s)	112, 242, 452, 605, 742, 1072, 1261, 1325, 1427
AhlI	ACTAGT	1 cut(s)	116
AjnI	CCWGG	2 cut(s)	227, 1362
Alw21I	GWGCWC	1 cut(s)	974
AlwI	GGATC	1 cut(s)	909
AlwNI	CAGNNNCTG	1 cut(s)	1363
AoxI	GGCC	5 cut(s)	224, 413, 436, 1031, 1344
ApeKI	GCWGC	5 cut(s)	127, 667, 773, 861, 1298
ApoI	RAATTY	4 cut(s)	552, 850, 1008, 1043
Asp718I	GGTACC	2 cut(s)	626, 948
AspLEI	GCGC	1 cut(s)	370
AspS9I	GGNCC	6 cut(s)	225, 413, 874, 1031, 1055, 1344
AsuHPI	GGTGA	2 cut(s)	52, 979
AvaII	GGWCC	2 cut(s)	874, 1055
BanI	GGYRCC	2 cut(s)	626, 948
BanII	GRGCYC	1 cut(s)	974
BbsI	GAAGAC	1 cut(s)	1332
Bbv12I	GWGCWC	1 cut(s)	974
BbvCI	CCTCAGC	2 cut(s)	233, 254
BbvI	GCAGC	5 cut(s)	114, 679, 760, 873, 1285
BccI	CCATC	2 cut(s)	256, 1386
BceAI	ACGGC	1 cut(s)	702
BciT130I	CCWGG	2 cut(s)	229, 1364
BclI	TGATCA	1 cut(s)	954
BcuI	ACTAGT	1 cut(s)	116
BfaI	CTAG	8 cut(s)	117, 324, 548, 671, 878, 923, 1124, 1214
BfrI	CTTAAG	1 cut(s)	986
BisI	GCNGC	6 cut(s)	128, 210, 668, 774, 862, 1299
BlsI	GCNGC	6 cut(s)	129, 211, 669, 775, 863, 1300
Bme1390I	CCNGG	2 cut(s)	229, 1364
Bme18I	GGWCC	2 cut(s)	874, 1055
BmgT120I	GGNCC	6 cut(s)	225, 413, 874, 1031, 1055, 1344
BmiI	GGNNCC	6 cut(s)	196, 337, 628, 875, 950, 1056
BmrFI	CCNGG	2 cut(s)	229, 1364
BmrI	ACTGGG	2 cut(s)	790, 926
BmsI	GCATC	4 cut(s)	388, 664, 1000, 1357
BmuI	ACTGGG	2 cut(s)	790, 926
BpiI	GAAGAC	1 cut(s)	1332
BpmI	CTGGAG	2 cut(s)	322, 662
Bpu10I	CCTNAGC	2 cut(s)	233, 254
BsaJI	CCNNGG	3 cut(s)	227, 363, 1287
BsaWI	WCCGGW	1 cut(s)	701
BsaXI	ACNNNNNCTCC	8 cut(s)	186, 206, 216, 236, 365, 395, 1083, 1113
Bsc4I	CCNNNNNNNGG	1 cut(s)	234
Bse1I	ACTGG	2 cut(s)	785, 921
BseBI	CCWGG	2 cut(s)	229, 1364
BseDI	CCNNGG	3 cut(s)	227, 363, 1287
BseGI	GGATG	5 cut(s)	174, 363, 850, 991, 1372
BseLI	CCNNNNNNNGG	1 cut(s)	234
BseMII	CTCAG	4 cut(s)	195, 224, 245, 747
BseNI	ACTGG	2 cut(s)	785, 921
BseRI	GAGGAG	1 cut(s)	207
BseXI	GCAGC	5 cut(s)	114, 679, 760, 873, 1285
BsgI	GTGCAG	1 cut(s)	1284
Bsh1236I	CGCG	1 cut(s)	95
BshFI	GGCC	5 cut(s)	226, 415, 438, 1033, 1346
BshNI	GGYRCC	2 cut(s)	626, 948
BsiHKAI	GWGCWC	1 cut(s)	974
BsiSI	CCGG	2 cut(s)	587, 702
BslFI	GGGAC	2 cut(s)	887, 1068
BslI	CCNNNNNNNGG	1 cut(s)	234
BsmFI	GGGAC	2 cut(s)	887, 1068
BsmI	GAATGC	1 cut(s)	1067
BsnI	GGCC	5 cut(s)	226, 415, 438, 1033, 1346
Bsp1286I	GDGCHC	1 cut(s)	974
Bsp1407I	TGTACA	1 cut(s)	316
Bsp143I	GATC	4 cut(s)	901, 954, 1038, 1273
Bsp19I	CCATGG	1 cut(s)	363
BspACI	CCGC	7 cut(s)	36, 63, 95, 209, 212, 864, 1230
BspANI	GGCC	5 cut(s)	226, 415, 438, 1033, 1346
BspCNI	CTCAG	4 cut(s)	194, 225, 246, 748
BspFNI	CGCG	1 cut(s)	95
BspLI	GGNNCC	6 cut(s)	196, 337, 628, 875, 950, 1056
BspPI	GGATC	1 cut(s)	909
BspT107I	GGYRCC	2 cut(s)	626, 948
BspTI	CTTAAG	1 cut(s)	986
BsrGI	TGTACA	1 cut(s)	316
BsrI	ACTGG	2 cut(s)	785, 921
BssECI	CCNNGG	3 cut(s)	227, 363, 1287
BssMI	GATC	4 cut(s)	901, 954, 1038, 1273
BssNAI	GTATAC	1 cut(s)	1333
BssT1I	CCWWGG	1 cut(s)	363
Bst1107I	GTATAC	1 cut(s)	1333
Bst2UI	CCWGG	2 cut(s)	229, 1364
Bst4CI	ACNGT	4 cut(s)	136, 1288, 1359, 1423
BstAFI	CTTAAG	1 cut(s)	986
BstAUI	TGTACA	1 cut(s)	316
BstC8I	GCNNGC	2 cut(s)	514, 665
BstDEI	CTNAG	5 cut(s)	181, 233, 254, 756, 1148
BstDSI	CCRYGG	2 cut(s)	363, 1287
BstF5I	GGATG	5 cut(s)	174, 363, 850, 991, 1372
BstFNI	CGCG	1 cut(s)	95
BstHHI	GCGC	1 cut(s)	370
BstKTI	GATC	4 cut(s)	904, 957, 1041, 1276
BstMBI	GATC	4 cut(s)	901, 954, 1038, 1273
BstMWI	GCNNNNNNNGC	3 cut(s)	513, 770, 1352
BstNI	CCWGG	2 cut(s)	229, 1364
BstNSI	RCATGY	4 cut(s)	389, 516, 667, 830
BstSCI	CCNGG	2 cut(s)	227, 1362
BstUI	CGCG	1 cut(s)	95
BstV1I	GCAGC	5 cut(s)	114, 679, 760, 873, 1285
BstV2I	GAAGAC	1 cut(s)	1332
BstX2I	RGATCY	1 cut(s)	901
BstXI	CCANNNNNNTGG	1 cut(s)	205
BstYI	RGATCY	1 cut(s)	901
BstZ17I	GTATAC	1 cut(s)	1333
BsuRI	GGCC	5 cut(s)	226, 415, 438, 1033, 1346
BtgI	CCRYGG	2 cut(s)	363, 1287
BtsCI	GGATG	5 cut(s)	174, 363, 850, 991, 1372
BtsI	GCAGTG	1 cut(s)	165
BtsIMutI	CAGTG	3 cut(s)	165, 914, 1191
Cac8I	GCNNGC	2 cut(s)	514, 665
CaiI	CAGNNNCTG	1 cut(s)	1363
CfoI	GCGC	1 cut(s)	370
Cfr13I	GGNCC	6 cut(s)	225, 413, 874, 1031, 1055, 1344
Csp6I	GTAC	5 cut(s)	317, 564, 627, 949, 1189
CspCI	CAANNNNNGTGG	2 cut(s)	469, 504
CviAII	CATG	7 cut(s)	52, 205, 364, 386, 513, 664, 827
CviQI	GTAC	5 cut(s)	317, 564, 627, 949, 1189
DdeI	CTNAG	5 cut(s)	181, 233, 254, 756, 1148
DpnI	GATC	4 cut(s)	903, 956, 1040, 1275
DpnII	GATC	4 cut(s)	901, 954, 1038, 1273
DraI	TTTAAA	1 cut(s)	85
EciI	GGCGGA	2 cut(s)	227, 1245
Ecl136II	GAGCTC	1 cut(s)	972
Eco130I	CCWWGG	1 cut(s)	363
Eco24I	GRGCYC	1 cut(s)	974
Eco32I	GATATC	2 cut(s)	344, 711
Eco47I	GGWCC	2 cut(s)	874, 1055
Eco53kI	GAGCTC	1 cut(s)	972
Eco57I	CTGAAG	1 cut(s)	786
EcoICRI	GAGCTC	1 cut(s)	972
EcoO109I	RGGNCCY	1 cut(s)	874
EcoRI	GAATTC	2 cut(s)	850, 1008
EcoRII	CCWGG	2 cut(s)	227, 1362
EcoRV	GATATC	2 cut(s)	344, 711
EcoT14I	CCWWGG	1 cut(s)	363
EcoT22I	ATGCAT	1 cut(s)	679
EcoT38I	GRGCYC	1 cut(s)	974
ErhI	CCWWGG	1 cut(s)	363
FaeI	CATG	7 cut(s)	55, 208, 367, 389, 516, 667, 830
FaqI	GGGAC	2 cut(s)	887, 1068
FatI	CATG	7 cut(s)	51, 204, 363, 385, 512, 663, 826
FbaI	TGATCA	1 cut(s)	954
FblI	GTMKAC	2 cut(s)	655, 1332
Fnu4HI	GCNGC	6 cut(s)	128, 210, 668, 774, 862, 1299
FokI	GGATG	5 cut(s)	181, 370, 857, 978, 1379
FriOI	GRGCYC	1 cut(s)	974
Fsp4HI	GCNGC	6 cut(s)	128, 210, 668, 774, 862, 1299
FspBI	CTAG	8 cut(s)	117, 324, 548, 671, 878, 923, 1124, 1214
GlaI	GCGC	1 cut(s)	369
GluI	GCNGC	6 cut(s)	128, 210, 668, 774, 862, 1299
GsuI	CTGGAG	2 cut(s)	322, 662
HaeIII	GGCC	5 cut(s)	226, 415, 438, 1033, 1346
HapII	CCGG	2 cut(s)	587, 702
HhaI	GCGC	1 cut(s)	370
Hin1II	CATG	7 cut(s)	55, 208, 367, 389, 516, 667, 830
Hin6I	GCGC	1 cut(s)	368
HinP1I	GCGC	1 cut(s)	368
HindIII	AAGCTT	1 cut(s)	983
HinfI	GANTC	7 cut(s)	98, 238, 617, 911, 1158, 1209, 1308
HpaII	CCGG	2 cut(s)	587, 702
HphI	GGTGA	2 cut(s)	52, 979
Hpy166II	GTNNAC	3 cut(s)	656, 1191, 1333
Hpy188III	TCNNGA	5 cut(s)	112, 339, 526, 855, 1312
Hpy8I	GTNNAC	3 cut(s)	656, 1191, 1333
Hpy99I	CGWCG	1 cut(s)	802
HpyAV	CCTTC	8 cut(s)	158, 510, 729, 835, 1090, 1220, 1231, 1378
HpyCH4III	ACNGT	4 cut(s)	136, 1288, 1359, 1423
HpyCH4IV	ACGT	2 cut(s)	797, 1329
HpyCH4V	TGCA	9 cut(s)	407, 512, 667, 677, 776, 891, 1301, 1355, 1370
HpyF10VI	GCNNNNNNNGC	3 cut(s)	513, 770, 1352
HpyF3I	CTNAG	5 cut(s)	181, 233, 254, 756, 1148
HpySE526I	ACGT	2 cut(s)	797, 1329
Hsp92II	CATG	7 cut(s)	55, 208, 367, 389, 516, 667, 830
HspAI	GCGC	1 cut(s)	368
KpnI	GGTACC	2 cut(s)	630, 952
Ksp22I	TGATCA	1 cut(s)	954
Kzo9I	GATC	4 cut(s)	901, 954, 1038, 1273
LmnI	GCTCC	3 cut(s)	194, 341, 643
Lsp1109I	GCAGC	5 cut(s)	114, 679, 760, 873, 1285
LweI	GCATC	4 cut(s)	388, 664, 1000, 1357
MaeI	CTAG	8 cut(s)	117, 324, 548, 671, 878, 923, 1124, 1214
MaeII	ACGT	2 cut(s)	797, 1329
MaeIII	GTNAC	2 cut(s)	5, 1291
MalI	GATC	4 cut(s)	903, 956, 1040, 1275
MboI	GATC	4 cut(s)	901, 954, 1038, 1273
MboII	GAAGA	8 cut(s)	271, 292, 295, 462, 612, 779, 797, 1337
MfeI	CAATTG	2 cut(s)	596, 958
MflI	RGATCY	1 cut(s)	901
MhlI	GDGCHC	1 cut(s)	974
MluCI	AATT	9 cut(s)	552, 570, 596, 832, 850, 958, 1008, 1043, 1281
Mph1103I	ATGCAT	1 cut(s)	679
MseI	TTAA	6 cut(s)	84, 174, 836, 987, 1020, 1410
MspA1I	CMGCKG	2 cut(s)	864, 1298
MspCI	CTTAAG	1 cut(s)	986
MspI	CCGG	2 cut(s)	587, 702
MspR9I	CCNGG	2 cut(s)	229, 1364
MunI	CAATTG	2 cut(s)	596, 958
Mva1269I	GAATGC	1 cut(s)	1067
MvaI	CCWGG	2 cut(s)	229, 1364
MvnI	CGCG	1 cut(s)	95
MwoI	GCNNNNNNNGC	3 cut(s)	513, 770, 1352
NcoI	CCATGG	1 cut(s)	363
NdeII	GATC	4 cut(s)	901, 954, 1038, 1273
NlaIII	CATG	7 cut(s)	55, 208, 367, 389, 516, 667, 830
NlaIV	GGNNCC	6 cut(s)	196, 337, 628, 875, 950, 1056
NsiI	ATGCAT	1 cut(s)	679
NspI	RCATGY	4 cut(s)	389, 516, 667, 830
PaeI	GCATGC	2 cut(s)	516, 667
PctI	GAATGC	1 cut(s)	1067
PfeI	GAWTC	7 cut(s)	98, 238, 617, 911, 1158, 1209, 1308
PfoI	TCCNGGA	1 cut(s)	1362
PkrI	GCNGC	6 cut(s)	129, 211, 669, 775, 863, 1300
PpuMI	RGGWCCY	1 cut(s)	874
Psp124BI	GAGCTC	1 cut(s)	974
Psp5II	RGGWCCY	1 cut(s)	874
Psp6I	CCWGG	2 cut(s)	227, 1362
PspGI	CCWGG	2 cut(s)	227, 1362
PspN4I	GGNNCC	6 cut(s)	196, 337, 628, 875, 950, 1056
PspPI	GGNCC	6 cut(s)	225, 413, 874, 1031, 1055, 1344
PspPPI	RGGWCCY	1 cut(s)	874
PsrI	GAACNNNNNNTAC	2 cut(s)	567, 599
PstNI	CAGNNNCTG	1 cut(s)	1363
PsuI	RGATCY	1 cut(s)	901
PvuII	CAGCTG	1 cut(s)	1298
RsaI	GTAC	5 cut(s)	318, 565, 628, 950, 1190
RsaNI	GTAC	5 cut(s)	317, 564, 627, 949, 1189
SacI	GAGCTC	1 cut(s)	974
SaqAI	TTAA	6 cut(s)	84, 174, 836, 987, 1020, 1410
SatI	GCNGC	6 cut(s)	128, 210, 668, 774, 862, 1299
Sau3AI	GATC	4 cut(s)	901, 954, 1038, 1273
Sau96I	GGNCC	6 cut(s)	225, 413, 874, 1031, 1055, 1344
ScrFI	CCNGG	2 cut(s)	229, 1364
SduI	GDGCHC	1 cut(s)	974
SfaNI	GCATC	4 cut(s)	388, 664, 1000, 1357
SinI	GGWCC	2 cut(s)	874, 1055
SmlI	CTYRAG	1 cut(s)	986
SmoI	CTYRAG	1 cut(s)	986
SpeI	ACTAGT	1 cut(s)	116
SphI	GCATGC	2 cut(s)	516, 667
Sse9I	AATT	9 cut(s)	552, 570, 596, 832, 850, 958, 1008, 1043, 1281
SsiI	CCGC	7 cut(s)	36, 63, 95, 209, 212, 864, 1230
SspI	AATATT	1 cut(s)	1141
SspMI	CTAG	8 cut(s)	117, 324, 548, 671, 878, 923, 1124, 1214
SstI	GAGCTC	1 cut(s)	974
StyD4I	CCNGG	2 cut(s)	227, 1362
StyI	CCWWGG	1 cut(s)	363
TaaI	ACNGT	4 cut(s)	136, 1288, 1359, 1423
TaiI	ACGT	2 cut(s)	800, 1332
TaqI	TCGA	2 cut(s)	792, 1047
TasI	AATT	9 cut(s)	552, 570, 596, 832, 850, 958, 1008, 1043, 1281
TatI	WGTACW	3 cut(s)	316, 563, 1188
TauI	GCSGC	1 cut(s)	212
TfiI	GAWTC	7 cut(s)	98, 238, 617, 911, 1158, 1209, 1308
Tru1I	TTAA	6 cut(s)	84, 174, 836, 987, 1020, 1410
Tru9I	TTAA	6 cut(s)	84, 174, 836, 987, 1020, 1410
TscAI	CASTG	3 cut(s)	165, 921, 1198
TseI	GCWGC	5 cut(s)	127, 667, 773, 861, 1298
TspDTI	ATGAA	1 cut(s)	854
TspGWI	ACGGA	1 cut(s)	1219
TspRI	CASTG	3 cut(s)	165, 921, 1198
Vha464I	CTTAAG	1 cut(s)	986
VpaK11BI	GGWCC	2 cut(s)	874, 1055
XapI	RAATTY	4 cut(s)	552, 850, 1008, 1043
XceI	RCATGY	4 cut(s)	389, 516, 667, 830
XmiI	GTMKAC	2 cut(s)	655, 1332
XspI	CTAG	8 cut(s)	117, 324, 548, 671, 878, 923, 1124, 1214
Zsp2I	ATGCAT	1 cut(s)	679

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.