Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0010125

Physical Location & Seq

Genomic Coordinates Reverse (-)

50969313 .. 50973750

Gene Structure 4438 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00256620.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1425 bp

ATGGCCAAGAACGTCGGAATTCTCGCCGTCGACATCTACTTCCCCCCGACCTCCTTGGAGGCACATGATGGAGCAAGCAAGGGGAAATACACTATTGGACTTGGACAAGATTGCATGGCGTTTTGTTCAGAAGTCGAAGATGTCATCTCAATGAGGAGCCTGGCTCTTGTTCAGAATGTATCATCATTTAACATTTGGGTTTTGATTTTCAGTTTGACAGTGGTCACAAACCTCCTTGAGAAGTATGGGATTGACCCAAAACAAATTGGTCGCCTGGAAGTTGGCAGCGAGACTGTGATAGACAAAAGCAAATCTATCAAGACCTTCCTGATGCAAATTTTTGAGGCTTACCTTTATGCTCTGCCCATTAAACATGGAAATACTGACATTGAAGGTGTTGACTCAACTAATGCATGCTATGGGGGAACTGCCGCTTTATTCAACTGTGTCAATTGGGTGGAGAGTAGCTCATGGGATGGACGCTATGGACTTGTTGTGTGCACTGACAGTGCGGTCTATGCAGAAGGACCAGCCAGGCCAACCGGTGGAGCAGCTGCTATTGCCTTGCTGATTGGACCTGATGCTCCTATTGCTTTTGAAAGCAAAATTAGGGGGAGTCATATGTCTCACGTCTATGACTTTTACAAGCCAGACCTTGCCAGTGAATATCCGGTTGTTGATGGTAAACTATCTCAAACATGTTATCTGATGGCCCTTGATTCTTGCTATAAGCAATTATGTAAAAAGTATGAGAAAATTGAAGGCAAACAATTTTCTATTGCTGATGCCGAGTATTTTGTATTTCATTCCCCATACAACAAGCTCGTACAAAAAAAAGCTTTGCTCGTTTGCTCCATTGATGAGGCTGCTAAAGAAAAGTTTGCACCATTCGCGAACCTATCTAGTGATGAGAGCTATGCAAGCCGGGATCTTGAAAAGGTAGCCCAGCAAGTTGCAAAGCCCCTATATGACGCGAAGGTGCAACCCACAACTCTGGTGCCAAAGCAAGTTGGAAACATGTACACTGCATCCCTTTATGCCGCATTTGCTTCCCTCCTTCACAACAAACATGCCTCATTGGATGGCAAGCGTGTGATACTGTTCTCATACGGAAGTGGCTCAACTGCAACCATGTTCTCGTTGCAACTCCGTGCCGGTCAACATCCCTTTAGCTTGGGCAACATTGCAGCTGTGATGAATGTTGGAGAGAAGTTGAAGTCAAGACATGAGTTCGCTCCAGAGAAGTTTTGTGAAATCATGAAGATCATGGAGCACAGGTATGGAGGCAAAGACTTTGTGACAAACAAGGACATTAGCCTTCTACTTCCAGGCACATTCTACCTCACTGAAGTCGACTCTAAGTATCGTAGATTCTATGCCCAGAAGTATGGCTGTGAGAATGGTGTGGTTGCCAATGGTCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004421 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006084 GO:0006139 GO:0006163 GO:0006629 GO:0006637 GO:0006644 GO:0006720 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0008654 GO:0009058 GO:0009117 GO:0009150 GO:0009240 GO:0009259 GO:0009506 GO:0009987 GO:0016740 GO:0016746 GO:0019287 GO:0019637 GO:0019693 GO:0030054 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0043603 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046490 GO:0046912 GO:0051186 GO:0055044 GO:0055086 GO:0071704 GO:0072521 GO:0090407 GO:1901135 GO:1901360 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00072 ko00280 ko00650 ko00900 ko01100 ko01110 ko01130 map00072 map00280 map00650 map00900 map01100 map01110 map01130

Pfam Domains

Protein Families

HMG_CoA_synt_C HMG_CoA_synt_N

Protein Analysis

474

Amino Acids

52.2

Weight (kDa)

6.61

Isoelectric Point (pI)

35.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMG_CoA_synt_N	PF01154	2 - 59	5.5e-12	Hydroxymethylglutaryl-coenzyme A synthase N terminal
HMG_CoA_synt_N	PF01154	70 - 196	1.8e-53	Hydroxymethylglutaryl-coenzyme A synthase N terminal
HMG_CoA_synt_C	PF08540	197 - 462	3.1e-100	Hydroxymethylglutaryl-coenzyme A synthase C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010125)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11820 AT4G11820 AT4G11820
fragaria_vesca	FvH4_5g01350 FvH4_5g01350
malus_domestica	MD04G1069200.v1.1 MD06G1070000.v1.1
prunus_persica	Prupe.5G088900_v2.0.a1 Prupe.5G088900_v2.0.a1
pyrus_communis	pycom04g06300 pycom06g07050
rosa_chinensis	RchiOBHm_Chr7g0199551
rosa_laevigata	RLG00000003786
rosa_multiflora	Rmu_sc0007070.1_g000002
rosa_roxburghii	Rroxscaffold_3G00256620
rosa_rugosa	Rorug07G0055300
rosa_samantha	Rh7AG180000 Rh7BG183200 Rh7CG189000 Rh7DG182700
rosa_wichuraiana	Rw7G015810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	512
AccB1I	GGYRCC	1 cut(s)	997
AccB7I	CCANNNNNTGG	1 cut(s)	545
AccI	GTMKAC	2 cut(s)	30, 1353
AccII	CGCG	2 cut(s)	893, 974
AciI	CCGC	3 cut(s)	432, 512, 1041
AclWI	GGATC	1 cut(s)	936
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	2 cut(s)	18, 336
AcuI	CTGAAG	1 cut(s)	1368
AfaI	GTAC	2 cut(s)	828, 1022
AfiI	CCNNNNNNNGG	1 cut(s)	545
AflIII	ACRYGT	2 cut(s)	698, 1017
AgeI	ACCGGT	1 cut(s)	542
AgsI	TTSAA	6 cut(s)	392, 442, 599, 761, 935, 1216
AjiI	CACGTC	1 cut(s)	631
AjnI	CCWGG	4 cut(s)	159, 273, 533, 1327
AluBI	AGCT	7 cut(s)	468, 554, 823, 839, 915, 1173, 1190
AluI	AGCT	7 cut(s)	468, 554, 823, 839, 915, 1173, 1190
Alw21I	GWGCWC	2 cut(s)	503, 1275
Alw26I	GTCTC	2 cut(s)	284, 630
Alw44I	GTGCAC	1 cut(s)	499
AlwI	GGATC	1 cut(s)	936
AoxI	GGCC	3 cut(s)	3, 536, 711
ApaLI	GTGCAC	1 cut(s)	499
ApeKI	GCWGC	5 cut(s)	285, 551, 554, 866, 1187
ApoI	RAATTY	2 cut(s)	18, 336
AsiGI	ACCGGT	1 cut(s)	542
AspS9I	GGNCC	3 cut(s)	527, 575, 712
AsuC2I	CCSGG	1 cut(s)	926
AvaII	GGWCC	2 cut(s)	527, 575
BaeGI	GKGCMC	1 cut(s)	503
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	1 cut(s)	997
Bbv12I	GWGCWC	2 cut(s)	503, 1275
BbvI	GCAGC	5 cut(s)	297, 541, 563, 853, 1199
BccI	CCATC	5 cut(s)	62, 470, 674, 703, 1076
BceAI	ACGGC	1 cut(s)	11
BciT130I	CCWGG	4 cut(s)	161, 275, 535, 1329
BcnI	CCSGG	1 cut(s)	926
BcoDI	GTCTC	2 cut(s)	284, 630
BfaI	CTAG	1 cut(s)	903
BisI	GCNGC	7 cut(s)	286, 432, 552, 555, 867, 1041, 1188
BlsI	GCNGC	7 cut(s)	287, 433, 553, 556, 868, 1042, 1189
Bme1390I	CCNGG	5 cut(s)	161, 275, 535, 926, 1329
Bme18I	GGWCC	2 cut(s)	527, 575
BmgBI	CACGTC	1 cut(s)	631
BmgT120I	GGNCC	3 cut(s)	527, 575, 712
BmiI	GGNNCC	2 cut(s)	158, 999
BmrFI	CCNGG	5 cut(s)	161, 275, 535, 926, 1329
BmsI	GCATC	4 cut(s)	321, 571, 775, 1037
BoxI	GACNNNNGTC	1 cut(s)	221
BplI	GAGNNNNNCTC	4 cut(s)	148, 180, 452, 484
BpmI	CTGGAG	1 cut(s)	1221
BpuEI	CTTGAG	1 cut(s)	257
BpuMI	CCSGG	1 cut(s)	926
BsaJI	CCNNGG	1 cut(s)	54
BsaWI	WCCGGW	2 cut(s)	542, 670
BsaXI	ACNNNNNCTCC	2 cut(s)	1262, 1292
Bsc4I	CCNNNNNNNGG	1 cut(s)	545
Bse118I	RCCGGY	2 cut(s)	542, 1154
Bse1I	ACTGG	1 cut(s)	660
Bse3DI	GCAATG	1 cut(s)	1182
BseBI	CCWGG	4 cut(s)	161, 275, 535, 1329
BseDI	CCNNGG	1 cut(s)	54
BseGI	GGATG	4 cut(s)	481, 1028, 1087, 1162
BseLI	CCNNNNNNNGG	1 cut(s)	545
BseMI	GCAATG	1 cut(s)	1182
BseNI	ACTGG	1 cut(s)	660
BseRI	GAGGAG	1 cut(s)	169
BseSI	GKGCMC	1 cut(s)	503
BseXI	GCAGC	5 cut(s)	297, 541, 563, 853, 1199
BseYI	CCCAGC	1 cut(s)	945
Bsh1236I	CGCG	2 cut(s)	893, 974
BshFI	GGCC	3 cut(s)	5, 538, 713
BshNI	GGYRCC	1 cut(s)	997
BshTI	ACCGGT	1 cut(s)	542
BsiHKAI	GWGCWC	2 cut(s)	503, 1275
BsiSI	CCGG	4 cut(s)	543, 671, 925, 1155
BslI	CCNNNNNNNGG	1 cut(s)	545
BsmAI	GTCTC	2 cut(s)	284, 630
BsnI	GGCC	3 cut(s)	5, 538, 713
Bsp1286I	GDGCHC	2 cut(s)	503, 1275
Bsp1407I	TGTACA	1 cut(s)	1020
Bsp143I	GATC	2 cut(s)	928, 1263
Bsp68I	TCGCGA	1 cut(s)	893
BspACI	CCGC	3 cut(s)	432, 512, 1041
BspANI	GGCC	3 cut(s)	5, 538, 713
BspFNI	CGCG	2 cut(s)	893, 974
BspHI	TCATGA	1 cut(s)	1257
BspLI	GGNNCC	2 cut(s)	158, 999
BspPI	GGATC	1 cut(s)	936
BspT107I	GGYRCC	1 cut(s)	997
BsrDI	GCAATG	1 cut(s)	1182
BsrFI	RCCGGY	2 cut(s)	542, 1154
BsrGI	TGTACA	1 cut(s)	1020
BsrI	ACTGG	1 cut(s)	660
BssAI	RCCGGY	2 cut(s)	542, 1154
BssECI	CCNNGG	1 cut(s)	54
BssMI	GATC	2 cut(s)	928, 1263
BssT1I	CCWWGG	1 cut(s)	54
Bst2UI	CCWGG	4 cut(s)	161, 275, 535, 1329
Bst4CI	ACNGT	5 cut(s)	220, 295, 446, 509, 1101
BstAUI	TGTACA	1 cut(s)	1020
BstC8I	GCNNGC	4 cut(s)	76, 415, 922, 1088
BstDEI	CTNAG	1 cut(s)	1359
BstF5I	GGATG	4 cut(s)	481, 1028, 1087, 1162
BstFNI	CGCG	2 cut(s)	893, 974
BstKTI	GATC	2 cut(s)	931, 1266
BstMAI	GTCTC	2 cut(s)	284, 630
BstMBI	GATC	2 cut(s)	928, 1263
BstMWI	GCNNNNNNNGC	6 cut(s)	518, 560, 590, 890, 921, 1046
BstNI	CCWGG	4 cut(s)	161, 275, 535, 1329
BstNSI	RCATGY	4 cut(s)	417, 702, 1021, 1073
BstPAI	GACNNNNGTC	1 cut(s)	221
BstSCI	CCNGG	5 cut(s)	159, 273, 533, 924, 1327
BstSLI	GKGCMC	1 cut(s)	503
BstUI	CGCG	2 cut(s)	893, 974
BstV1I	GCAGC	5 cut(s)	297, 541, 563, 853, 1199
BstX2I	RGATCY	1 cut(s)	928
BstXI	CCANNNNNNTGG	2 cut(s)	994, 1388
BstYI	RGATCY	1 cut(s)	928
BsuRI	GGCC	3 cut(s)	5, 538, 713
BtrI	CACGTC	1 cut(s)	631
BtsCI	GGATG	4 cut(s)	481, 1028, 1087, 1162
BtsI	GCAGTG	1 cut(s)	1023
BtsIMutI	CAGTG	7 cut(s)	225, 501, 514, 667, 1023, 1344, 1420
BtuMI	TCGCGA	1 cut(s)	893
Cac8I	GCNNGC	4 cut(s)	76, 415, 922, 1088
CciI	TCATGA	1 cut(s)	1257
Cfr10I	RCCGGY	2 cut(s)	542, 1154
Cfr13I	GGNCC	3 cut(s)	527, 575, 712
CseI	GACGC	2 cut(s)	489, 980
Csp6I	GTAC	2 cut(s)	827, 1021
CspAI	ACCGGT	1 cut(s)	542
CviQI	GTAC	2 cut(s)	827, 1021
DdeI	CTNAG	1 cut(s)	1359
DpnI	GATC	2 cut(s)	930, 1265
DpnII	GATC	2 cut(s)	928, 1263
DrdI	GACNNNNNNGTC	1 cut(s)	512
DseDI	GACNNNNNNGTC	1 cut(s)	512
EaeI	YGGCCR	1 cut(s)	3
Eco130I	CCWWGG	1 cut(s)	54
Eco47I	GGWCC	2 cut(s)	527, 575
Eco57I	CTGAAG	1 cut(s)	1368
EcoRI	GAATTC	1 cut(s)	18
EcoRII	CCWGG	4 cut(s)	159, 273, 533, 1327
EcoT14I	CCWWGG	1 cut(s)	54
EcoT22I	ATGCAT	1 cut(s)	415
ErhI	CCWWGG	1 cut(s)	54
FauNDI	CATATG	1 cut(s)	621
FblI	GTMKAC	2 cut(s)	30, 1353
Fnu4HI	GCNGC	7 cut(s)	286, 432, 552, 555, 867, 1041, 1188
FokI	GGATG	4 cut(s)	488, 1015, 1094, 1149
Fsp4HI	GCNGC	7 cut(s)	286, 432, 552, 555, 867, 1041, 1188
FspBI	CTAG	1 cut(s)	903
GluI	GCNGC	7 cut(s)	286, 432, 552, 555, 867, 1041, 1188
GsaI	CCCAGC	1 cut(s)	949
GsuI	CTGGAG	1 cut(s)	1221
HaeIII	GGCC	3 cut(s)	5, 538, 713
HapII	CCGG	4 cut(s)	543, 671, 925, 1155
HgaI	GACGC	2 cut(s)	489, 980
HincII	GTYRAC	4 cut(s)	31, 400, 1160, 1354
HindII	GTYRAC	4 cut(s)	31, 400, 1160, 1354
HindIII	AAGCTT	1 cut(s)	837
HinfI	GANTC	5 cut(s)	401, 616, 719, 1355, 1371
HpaII	CCGG	4 cut(s)	543, 671, 925, 1155
Hpy166II	GTNNAC	7 cut(s)	31, 400, 501, 686, 1023, 1160, 1354
Hpy188I	TCNGA	4 cut(s)	17, 130, 174, 708
Hpy188III	TCNNGA	7 cut(s)	319, 328, 892, 932, 1221, 1238, 1258
Hpy8I	GTNNAC	7 cut(s)	31, 400, 501, 686, 1023, 1160, 1354
Hpy99I	CGWCG	2 cut(s)	17, 32
HpyAV	CCTTC	7 cut(s)	334, 386, 518, 755, 970, 1067, 1328
HpyCH4III	ACNGT	5 cut(s)	220, 295, 446, 509, 1101
HpyCH4IV	ACGT	2 cut(s)	12, 630
HpyF10VI	GCNNNNNNNGC	6 cut(s)	518, 560, 590, 890, 921, 1046
HpyF3I	CTNAG	1 cut(s)	1359
HpySE526I	ACGT	2 cut(s)	12, 630
Kzo9I	GATC	2 cut(s)	928, 1263
LmnI	GCTCC	7 cut(s)	71, 156, 548, 589, 857, 1240, 1270
Lsp1109I	GCAGC	5 cut(s)	297, 541, 563, 853, 1199
LweI	GCATC	4 cut(s)	321, 571, 775, 1037
MaeI	CTAG	1 cut(s)	903
MaeII	ACGT	2 cut(s)	12, 630
MaeIII	GTNAC	3 cut(s)	223, 1297, 1418
MalI	GATC	2 cut(s)	930, 1265
MboI	GATC	2 cut(s)	928, 1263
MboII	GAAGA	2 cut(s)	149, 1273
MfeI	CAATTG	1 cut(s)	451
MflI	RGATCY	1 cut(s)	928
MhlI	GDGCHC	2 cut(s)	503, 1275
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	8 cut(s)	18, 264, 336, 451, 606, 734, 756, 770
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	3 cut(s)	395, 625, 1349
MmeI	TCCRAC	2 cut(s)	991, 1183
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	415
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	2 cut(s)	189, 369
MslI	CAYNNNNRTG	3 cut(s)	149, 633, 1278
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	2 cut(s)	554, 1190
MspI	CCGG	4 cut(s)	543, 671, 925, 1155
MspR9I	CCNGG	5 cut(s)	161, 275, 535, 926, 1329
MunI	CAATTG	1 cut(s)	451
MvaI	CCWGG	4 cut(s)	161, 275, 535, 1329
MvnI	CGCG	2 cut(s)	893, 974
MwoI	GCNNNNNNNGC	6 cut(s)	518, 560, 590, 890, 921, 1046
NciI	CCSGG	1 cut(s)	926
NdeI	CATATG	1 cut(s)	621
NdeII	GATC	2 cut(s)	928, 1263
NlaIV	GGNNCC	2 cut(s)	158, 999
NmeAIII	GCCGAG	1 cut(s)	814
NmuCI	GTSAC	3 cut(s)	223, 1297, 1418
NruI	TCGCGA	1 cut(s)	893
NsiI	ATGCAT	1 cut(s)	415
NspI	RCATGY	4 cut(s)	417, 702, 1021, 1073
PaeI	GCATGC	1 cut(s)	417
PagI	TCATGA	1 cut(s)	1257
PciI	ACATGT	2 cut(s)	698, 1017
PfeI	GAWTC	2 cut(s)	719, 1371
PflMI	CCANNNNNTGG	1 cut(s)	545
PinAI	ACCGGT	1 cut(s)	542
PkrI	GCNGC	7 cut(s)	287, 433, 553, 556, 868, 1042, 1189
PleI	GAGTC	3 cut(s)	395, 624, 1349
PpsI	GAGTC	3 cut(s)	395, 624, 1349
PscI	ACATGT	2 cut(s)	698, 1017
PshAI	GACNNNNGTC	1 cut(s)	221
Psp6I	CCWGG	4 cut(s)	159, 273, 533, 1327
PspFI	CCCAGC	1 cut(s)	945
PspGI	CCWGG	4 cut(s)	159, 273, 533, 1327
PspN4I	GGNNCC	2 cut(s)	158, 999
PspPI	GGNCC	3 cut(s)	527, 575, 712
PsuI	RGATCY	1 cut(s)	928
PvuII	CAGCTG	2 cut(s)	554, 1190
RruI	TCGCGA	1 cut(s)	893
RsaI	GTAC	2 cut(s)	828, 1022
RsaNI	GTAC	2 cut(s)	827, 1021
RseI	CAYNNNNRTG	3 cut(s)	149, 633, 1278
SalI	GTCGAC	2 cut(s)	29, 1352
SaqAI	TTAA	2 cut(s)	189, 369
SatI	GCNGC	7 cut(s)	286, 432, 552, 555, 867, 1041, 1188
Sau3AI	GATC	2 cut(s)	928, 1263
Sau96I	GGNCC	3 cut(s)	527, 575, 712
SchI	GAGTC	3 cut(s)	395, 625, 1349
ScrFI	CCNGG	5 cut(s)	161, 275, 535, 926, 1329
SduI	GDGCHC	2 cut(s)	503, 1275
SfaNI	GCATC	4 cut(s)	321, 571, 775, 1037
SinI	GGWCC	2 cut(s)	527, 575
SmiMI	CAYNNNNRTG	3 cut(s)	149, 633, 1278
SmlI	CTYRAG	1 cut(s)	236
SmoI	CTYRAG	1 cut(s)	236
SphI	GCATGC	1 cut(s)	417
Sse9I	AATT	8 cut(s)	18, 264, 336, 451, 606, 734, 756, 770
SsiI	CCGC	3 cut(s)	432, 512, 1041
SspMI	CTAG	1 cut(s)	903
StyD4I	CCNGG	5 cut(s)	159, 273, 533, 924, 1327
StyI	CCWWGG	1 cut(s)	54
TaaI	ACNGT	5 cut(s)	220, 295, 446, 509, 1101
TaiI	ACGT	2 cut(s)	15, 633
TaqI	TCGA	3 cut(s)	30, 135, 1353
TasI	AATT	8 cut(s)	18, 264, 336, 451, 606, 734, 756, 770
TatI	WGTACW	1 cut(s)	1020
TauI	GCSGC	2 cut(s)	434, 1043
TfiI	GAWTC	2 cut(s)	719, 1371
Tru1I	TTAA	2 cut(s)	189, 369
Tru9I	TTAA	2 cut(s)	189, 369
TscAI	CASTG	6 cut(s)	225, 508, 514, 667, 1030, 1351
TseFI	GTSAC	3 cut(s)	223, 1297, 1418
TseI	GCWGC	5 cut(s)	285, 551, 554, 866, 1187
Tsp45I	GTSAC	3 cut(s)	223, 1297, 1418
TspDTI	ATGAA	3 cut(s)	794, 1211, 1274
TspGWI	ACGGA	2 cut(s)	1125, 1139
TspRI	CASTG	6 cut(s)	225, 508, 514, 667, 1030, 1351
Van91I	CCANNNNNTGG	1 cut(s)	545
VneI	GTGCAC	1 cut(s)	499
VpaK11BI	GGWCC	2 cut(s)	527, 575
XapI	RAATTY	2 cut(s)	18, 336
XceI	RCATGY	4 cut(s)	417, 702, 1021, 1073
XmiI	GTMKAC	2 cut(s)	30, 1353
XspI	CTAG	1 cut(s)	903
Zsp2I	ATGCAT	1 cut(s)	415

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.