Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0010125

Physical Location & Seq

Genomic Coordinates Forward (+)

16956857 .. 16961198

Gene Structure 4342 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1070000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1398 bp

ATGGCCAAAAACGTCGGAATTCTCGCCGTCGACATCTACTTCCCCCCTACCTGTATTCAGCAGGAAGCCTTGGAAGCTCATGATGGTGCCAGCAAAGGGAAGTACACTATCGGACTTGGACAAGATTGCATGGCGTTTTGTTCAGAGGTGGAAGATGTCATCTCAATGAGTTTGACAGTGGTTACCTCCCTTCTTGAGAAATATGGAATTGATCCAAAACAAATTGGTCGCCTCGAAGTAGGAAGCGAGACTGTGATTGACAAAAGCAAATCTATTAAGACCTTCCTGATGCCAATTTTTGAGAAACATGGAAATACTGACATTGAAGGTGTTGACTCAACTAATGCATGCTATGGGGGAACTGCAGCTTTATTCAACTGTGTCAATTGGGTGGAGAGTAGCTCTTGGGATGGACGCTATGGGCTTGTCGTGTGCACTGACAGTGCGGTCTATGCAGAAGGACCTGCACGACCTACTGGTGGAGCAGCTGCTATTGCCATGCTGATTGGACCTGATGCTCCTATTTCTTTTGAGAGCAAAATTAGGGGAAGTCATATGGCTCATGTGTATGATTTTTACAAGCCCGACCTTGCTAGTGAATATCCGGTTGTTGATGGAAAGCTTTCTCAAACATGTTACCTGAAGGCTCTTGATTCTTGCTACAAGGAATTATGTAAAAAGTATGAGAAATTGGAAGGTAAACAATTTTCTATCGCTGACGCTGAGTATTTCGTATTTCATTCTCCATATAACAAGCTCGTACAAAAAAGCTTTGCTCGTTTGGTGTTCAATGACTCCGTAAGAAATGCCAGCTCCATTGATGAGGCCGGGAAAGAAAAGCTGGCTCCATTTGCTGACCTTCCTGGTGATGATAGCTACAATAGCCGGGATCTTGAAAAGGCATCCCAGCAAGTCGCAAAGCCTCTCTACGATGCTAAAGTGGTACCTACAACTTTGGTACCAAAGCAAGTTGGCAACATGTACACTGCATCTATTTATGCAGCATTTGTATCCCTCCTTCACAACAAACACAGCTCATTGGATGGCAAGCGGGTGATACTGTTCTCTTACGGAAGTGGCTCAGCTGCTACCATGTTCTCATTGTGTCTCAATGCTGGTCAACAGCCCTTTAGCTTGGAGAACATTGCAGCCGTTATGAATGTTGGAGAGAAGTTGAAAGCAAGACATGAGTTCCCTCCCGAGAAGTTTGTTGAAGTGATGAAGATCATGGAGCACAGGTATGGAGGGAAAGACTTTGTGACCAACCAGGATATCAGCCTTCTACTTCCAGGCACATACTATCTTACTGAAGTCGACTCTAAGTATCGTAGATTCTATGGCAAGAAGGATGCCGCTGCCACCTCTGATGACAATGGTGTGGTTGCCAACTGTCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004421 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006084 GO:0006139 GO:0006163 GO:0006629 GO:0006637 GO:0006644 GO:0006720 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0008654 GO:0009058 GO:0009117 GO:0009150 GO:0009240 GO:0009259 GO:0009506 GO:0009987 GO:0016740 GO:0016746 GO:0019287 GO:0019637 GO:0019693 GO:0030054 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0043603 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046490 GO:0046912 GO:0051186 GO:0055044 GO:0055086 GO:0071704 GO:0072521 GO:0090407 GO:1901135 GO:1901360 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00072 ko00280 ko00650 ko00900 ko01100 ko01110 ko01130 map00072 map00280 map00650 map00900 map01100 map01110 map01130

Pfam Domains

Protein Families

HMG_CoA_synt_C HMG_CoA_synt_N

Protein Analysis

466

Amino Acids

50.95

Weight (kDa)

5.66

Isoelectric Point (pI)

34.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMG_CoA_synt_N	PF01154	2 - 174	1.5e-80	Hydroxymethylglutaryl-coenzyme A synthase N terminal
HMG_CoA_synt_C	PF08540	175 - 449	8e-111	Hydroxymethylglutaryl-coenzyme A synthase C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010125)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11820 AT4G11820 AT4G11820
fragaria_vesca	FvH4_5g01350 FvH4_5g01350
malus_domestica	MD04G1069200.v1.1 MD06G1070000.v1.1
prunus_persica	Prupe.5G088900_v2.0.a1 Prupe.5G088900_v2.0.a1
pyrus_communis	pycom04g06300 pycom06g07050
rosa_chinensis	RchiOBHm_Chr7g0199551
rosa_laevigata	RLG00000003786
rosa_multiflora	Rmu_sc0007070.1_g000002
rosa_roxburghii	Rroxscaffold_3G00256620
rosa_rugosa	Rorug07G0055300
rosa_samantha	Rh7AG180000 Rh7BG183200 Rh7CG189000 Rh7DG182700
rosa_wichuraiana	Rw7G015810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	446
Acc36I	ACCTGC	1 cut(s)	472
Acc65I	GGTACC	2 cut(s)	943, 958
AccB1I	GGYRCC	3 cut(s)	86, 943, 958
AccI	GTMKAC	2 cut(s)	30, 1314
AciI	CCGC	3 cut(s)	446, 1051, 1353
AclWI	GGATC	2 cut(s)	206, 897
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	1 cut(s)	18
AcuI	CTGAAG	2 cut(s)	662, 1329
AfaI	GTAC	5 cut(s)	104, 762, 945, 960, 983
AfiI	CCNNNNNNNGG	2 cut(s)	96, 479
AflIII	ACRYGT	2 cut(s)	632, 978
AgsI	TTSAA	6 cut(s)	326, 376, 790, 896, 1177, 1214
AjnI	CCWGG	3 cut(s)	862, 1266, 1288
Alw21I	GWGCWC	2 cut(s)	437, 1236
Alw26I	GTCTC	2 cut(s)	242, 1112
Alw44I	GTGCAC	1 cut(s)	433
AlwI	GGATC	2 cut(s)	206, 897
Ama87I	CYCGRG	1 cut(s)	1199
AoxI	GGCC	2 cut(s)	3, 825
ApaLI	GTGCAC	1 cut(s)	433
ApeKI	GCWGC	7 cut(s)	365, 485, 488, 1001, 1085, 1148, 1355
ApoI	RAATTY	1 cut(s)	18
Asp700I	GAANNNNTTC	1 cut(s)	622
Asp718I	GGTACC	2 cut(s)	943, 958
AspS9I	GGNCC	2 cut(s)	461, 509
AsuC2I	CCSGG	2 cut(s)	829, 887
AsuHPI	GGTGA	2 cut(s)	878, 1066
AvaI	CYCGRG	1 cut(s)	1199
AvaII	GGWCC	2 cut(s)	461, 509
BaeGI	GKGCMC	1 cut(s)	437
BaeI	ACNNNNGTAYC	2 cut(s)	942, 975
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	3 cut(s)	86, 943, 958
BarI	GAAGNNNNNNTAC	2 cut(s)	1002, 1034
Bbv12I	GWGCWC	2 cut(s)	437, 1236
BbvI	GCAGC	7 cut(s)	377, 475, 497, 1013, 1072, 1160, 1342
BccI	CCATC	4 cut(s)	77, 404, 608, 1037
BceAI	ACGGC	2 cut(s)	11, 1136
BciT130I	CCWGG	3 cut(s)	864, 1268, 1290
BciVI	GTATCC	1 cut(s)	1021
BcnI	CCSGG	2 cut(s)	829, 887
BcoDI	GTCTC	2 cut(s)	242, 1112
BfaI	CTAG	1 cut(s)	594
BfmI	CTRYAG	1 cut(s)	363
BfuAI	ACCTGC	1 cut(s)	472
BfuI	GTATCC	1 cut(s)	1021
BisI	GCNGC	8 cut(s)	366, 486, 489, 1002, 1086, 1149, 1353, 1356
BlpI	GCTNAGC	1 cut(s)	1081
BlsI	GCNGC	8 cut(s)	367, 487, 490, 1003, 1087, 1150, 1354, 1357
Bme1390I	CCNGG	5 cut(s)	829, 864, 887, 1268, 1290
Bme18I	GGWCC	2 cut(s)	461, 509
BmeT110I	CYCGRG	1 cut(s)	1199
BmgT120I	GGNCC	2 cut(s)	461, 509
BmiI	GGNNCC	4 cut(s)	88, 846, 945, 960
BmrFI	CCNGG	5 cut(s)	829, 864, 887, 1268, 1290
BmsI	GCATC	6 cut(s)	279, 505, 911, 922, 998, 1339
BplI	GAGNNNNNCTC	2 cut(s)	386, 418
Bpu1102I	GCTNAGC	1 cut(s)	1081
BpuEI	CTTGAG	1 cut(s)	215
BpuMI	CCSGG	2 cut(s)	829, 887
BsaBI	GATNNNNATC	1 cut(s)	1223
BsaJI	CCNNGG	1 cut(s)	69
BsaWI	WCCGGW	1 cut(s)	604
BsaXI	ACNNNNNCTCC	2 cut(s)	1223, 1253
Bsc4I	CCNNNNNNNGG	2 cut(s)	96, 479
Bse1I	ACTGG	1 cut(s)	481
Bse3DI	GCAATG	1 cut(s)	1143
Bse8I	GATNNNNATC	1 cut(s)	1223
BseBI	CCWGG	3 cut(s)	864, 1268, 1290
BseDI	CCNNGG	1 cut(s)	69
BseGI	GGATG	4 cut(s)	415, 902, 1048, 1354
BseJI	GATNNNNATC	1 cut(s)	1223
BseLI	CCNNNNNNNGG	2 cut(s)	96, 479
BseMI	GCAATG	1 cut(s)	1143
BseMII	CTCAG	2 cut(s)	714, 1095
BseNI	ACTGG	1 cut(s)	481
BseSI	GKGCMC	1 cut(s)	437
BseXI	GCAGC	7 cut(s)	377, 475, 497, 1013, 1072, 1160, 1342
BseYI	CCCAGC	1 cut(s)	906
BsgI	GTGCAG	1 cut(s)	450
BshFI	GGCC	2 cut(s)	5, 827
BshNI	GGYRCC	3 cut(s)	86, 943, 958
BsiHKAI	GWGCWC	2 cut(s)	437, 1236
BsiHKCI	CYCGRG	1 cut(s)	1199
BsiSI	CCGG	3 cut(s)	605, 828, 886
BslI	CCNNNNNNNGG	2 cut(s)	96, 479
BsmAI	GTCTC	2 cut(s)	242, 1112
BsnI	GGCC	2 cut(s)	5, 827
BsoBI	CYCGRG	1 cut(s)	1199
Bsp1286I	GDGCHC	2 cut(s)	437, 1236
Bsp1407I	TGTACA	1 cut(s)	981
Bsp143I	GATC	3 cut(s)	211, 889, 1224
Bsp1720I	GCTNAGC	1 cut(s)	1081
BspACI	CCGC	3 cut(s)	446, 1051, 1353
BspANI	GGCC	2 cut(s)	5, 827
BspCNI	CTCAG	2 cut(s)	715, 1094
BspHI	TCATGA	1 cut(s)	79
BspLI	GGNNCC	4 cut(s)	88, 846, 945, 960
BspMAI	CTGCAG	1 cut(s)	367
BspMI	ACCTGC	1 cut(s)	472
BspPI	GGATC	2 cut(s)	206, 897
BspT107I	GGYRCC	3 cut(s)	86, 943, 958
BsrDI	GCAATG	1 cut(s)	1143
BsrGI	TGTACA	1 cut(s)	981
BsrI	ACTGG	1 cut(s)	481
BssECI	CCNNGG	1 cut(s)	69
BssMI	GATC	3 cut(s)	211, 889, 1224
BssT1I	CCWWGG	1 cut(s)	69
Bst2UI	CCWGG	3 cut(s)	864, 1268, 1290
Bst4CI	ACNGT	6 cut(s)	178, 253, 380, 443, 1062, 1391
BstAUI	TGTACA	1 cut(s)	981
BstC8I	GCNNGC	5 cut(s)	91, 349, 811, 843, 1049
BstDEI	CTNAG	3 cut(s)	723, 1081, 1320
BstEII	GGTNACC	1 cut(s)	181
BstF5I	GGATG	4 cut(s)	415, 902, 1048, 1354
BstKTI	GATC	3 cut(s)	214, 892, 1227
BstMAI	GTCTC	2 cut(s)	242, 1112
BstMBI	GATC	3 cut(s)	211, 889, 1224
BstMWI	GCNNNNNNNGC	5 cut(s)	74, 452, 494, 851, 882
BstNI	CCWGG	3 cut(s)	864, 1268, 1290
BstNSI	RCATGY	3 cut(s)	351, 636, 982
BstPI	GGTNACC	1 cut(s)	181
BstSCI	CCNGG	5 cut(s)	827, 862, 885, 1266, 1288
BstSFI	CTRYAG	1 cut(s)	363
BstSLI	GKGCMC	1 cut(s)	437
BstV1I	GCAGC	7 cut(s)	377, 475, 497, 1013, 1072, 1160, 1342
BstX2I	RGATCY	1 cut(s)	889
BstYI	RGATCY	1 cut(s)	889
BsuI	GTATCC	1 cut(s)	1021
BsuRI	GGCC	2 cut(s)	5, 827
BtsCI	GGATG	4 cut(s)	415, 902, 1048, 1354
BtsI	GCAGTG	1 cut(s)	984
BtsIMutI	CAGTG	5 cut(s)	183, 435, 448, 984, 1393
BveI	ACCTGC	1 cut(s)	472
Cac8I	GCNNGC	5 cut(s)	91, 349, 811, 843, 1049
CciI	TCATGA	1 cut(s)	79
Cfr13I	GGNCC	2 cut(s)	461, 509
CseI	GACGC	2 cut(s)	423, 728
Csp6I	GTAC	5 cut(s)	103, 761, 944, 959, 982
CviQI	GTAC	5 cut(s)	103, 761, 944, 959, 982
DdeI	CTNAG	3 cut(s)	723, 1081, 1320
DpnI	GATC	3 cut(s)	213, 891, 1226
DpnII	GATC	3 cut(s)	211, 889, 1224
DrdI	GACNNNNNNGTC	1 cut(s)	446
DseDI	GACNNNNNNGTC	1 cut(s)	446
EaeI	YGGCCR	1 cut(s)	3
Eco130I	CCWWGG	1 cut(s)	69
Eco32I	GATATC	1 cut(s)	1273
Eco47I	GGWCC	2 cut(s)	461, 509
Eco57I	CTGAAG	2 cut(s)	662, 1329
Eco88I	CYCGRG	1 cut(s)	1199
Eco91I	GGTNACC	1 cut(s)	181
EcoO109I	RGGNCCY	1 cut(s)	461
EcoO65I	GGTNACC	1 cut(s)	181
EcoRI	GAATTC	1 cut(s)	18
EcoRII	CCWGG	3 cut(s)	862, 1266, 1288
EcoRV	GATATC	1 cut(s)	1273
EcoT14I	CCWWGG	1 cut(s)	69
EcoT22I	ATGCAT	1 cut(s)	349
ErhI	CCWWGG	1 cut(s)	69
FauI	CCCGC	1 cut(s)	1044
FauNDI	CATATG	1 cut(s)	555
FblI	GTMKAC	2 cut(s)	30, 1314
Fnu4HI	GCNGC	8 cut(s)	366, 486, 489, 1002, 1086, 1149, 1353, 1356
FokI	GGATG	4 cut(s)	422, 889, 1055, 1361
Fsp4HI	GCNGC	8 cut(s)	366, 486, 489, 1002, 1086, 1149, 1353, 1356
FspBI	CTAG	1 cut(s)	594
GluI	GCNGC	8 cut(s)	366, 486, 489, 1002, 1086, 1149, 1353, 1356
GsaI	CCCAGC	1 cut(s)	910
HaeIII	GGCC	2 cut(s)	5, 827
HapII	CCGG	3 cut(s)	605, 828, 886
HgaI	GACGC	2 cut(s)	423, 728
HincII	GTYRAC	4 cut(s)	31, 334, 1121, 1315
HindII	GTYRAC	4 cut(s)	31, 334, 1121, 1315
HindIII	AAGCTT	2 cut(s)	620, 769
HinfI	GANTC	5 cut(s)	335, 653, 794, 1316, 1332
HpaII	CCGG	3 cut(s)	605, 828, 886
HphI	GGTGA	2 cut(s)	878, 1066
Hpy166II	GTNNAC	8 cut(s)	31, 105, 334, 435, 701, 984, 1121, 1315
Hpy188I	TCNGA	4 cut(s)	17, 113, 145, 1366
Hpy188III	TCNNGA	6 cut(s)	80, 194, 286, 650, 893, 1199
Hpy8I	GTNNAC	8 cut(s)	31, 105, 334, 435, 701, 984, 1121, 1315
Hpy99I	CGWCG	2 cut(s)	17, 32
HpyCH4III	ACNGT	6 cut(s)	178, 253, 380, 443, 1062, 1391
HpyCH4IV	ACGT	1 cut(s)	12
HpyCH4V	TGCA	9 cut(s)	129, 347, 365, 435, 455, 467, 989, 1001, 1148
HpyF10VI	GCNNNNNNNGC	5 cut(s)	74, 452, 494, 851, 882
HpyF3I	CTNAG	3 cut(s)	723, 1081, 1320
HpySE526I	ACGT	1 cut(s)	12
KpnI	GGTACC	2 cut(s)	947, 962
Kzo9I	GATC	3 cut(s)	211, 889, 1224
LmnI	GCTCC	5 cut(s)	482, 523, 818, 850, 1231
Lsp1109I	GCAGC	7 cut(s)	377, 475, 497, 1013, 1072, 1160, 1342
LweI	GCATC	6 cut(s)	279, 505, 911, 922, 998, 1339
MaeI	CTAG	1 cut(s)	594
MaeII	ACGT	1 cut(s)	12
MaeIII	GTNAC	4 cut(s)	181, 635, 1258, 1391
MalI	GATC	3 cut(s)	213, 891, 1226
MboI	GATC	3 cut(s)	211, 889, 1224
MboII	GAAGA	2 cut(s)	164, 1234
MfeI	CAATTG	1 cut(s)	385
MflI	RGATCY	1 cut(s)	889
MhlI	GDGCHC	2 cut(s)	437, 1236
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	9 cut(s)	18, 207, 222, 294, 385, 540, 668, 689, 704
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	3 cut(s)	329, 788, 1310
MmeI	TCCRAC	1 cut(s)	1144
MnlI	CCTC	9 cut(s)	139, 196, 242, 817, 933, 1025, 1206, 1238, 1372
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	349
MroXI	GAANNNNTTC	1 cut(s)	622
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	1 cut(s)	276
MslI	CAYNNNNRTG	4 cut(s)	84, 164, 567, 1239
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	3 cut(s)	488, 1085, 1355
MspI	CCGG	3 cut(s)	605, 828, 886
MspR9I	CCNGG	5 cut(s)	829, 864, 887, 1268, 1290
MunI	CAATTG	1 cut(s)	385
MvaI	CCWGG	3 cut(s)	864, 1268, 1290
MwoI	GCNNNNNNNGC	5 cut(s)	74, 452, 494, 851, 882
NciI	CCSGG	2 cut(s)	829, 887
NdeI	CATATG	1 cut(s)	555
NdeII	GATC	3 cut(s)	211, 889, 1224
NlaIV	GGNNCC	4 cut(s)	88, 846, 945, 960
NmuCI	GTSAC	2 cut(s)	1258, 1391
NsiI	ATGCAT	1 cut(s)	349
NspI	RCATGY	3 cut(s)	351, 636, 982
PaeI	GCATGC	1 cut(s)	351
PagI	TCATGA	1 cut(s)	79
PciI	ACATGT	2 cut(s)	632, 978
PdmI	GAANNNNTTC	1 cut(s)	622
PfeI	GAWTC	2 cut(s)	653, 1332
PkrI	GCNGC	8 cut(s)	367, 487, 490, 1003, 1087, 1150, 1354, 1357
PleI	GAGTC	3 cut(s)	329, 788, 1310
PpsI	GAGTC	3 cut(s)	329, 788, 1310
PpuMI	RGGWCCY	1 cut(s)	461
PscI	ACATGT	2 cut(s)	632, 978
Psp5II	RGGWCCY	1 cut(s)	461
Psp6I	CCWGG	3 cut(s)	862, 1266, 1288
PspEI	GGTNACC	1 cut(s)	181
PspFI	CCCAGC	1 cut(s)	906
PspGI	CCWGG	3 cut(s)	862, 1266, 1288
PspN4I	GGNNCC	4 cut(s)	88, 846, 945, 960
PspPI	GGNCC	2 cut(s)	461, 509
PspPPI	RGGWCCY	1 cut(s)	461
PstI	CTGCAG	1 cut(s)	367
PsuI	RGATCY	1 cut(s)	889
PvuII	CAGCTG	2 cut(s)	488, 1085
RsaI	GTAC	5 cut(s)	104, 762, 945, 960, 983
RsaNI	GTAC	5 cut(s)	103, 761, 944, 959, 982
RseI	CAYNNNNRTG	4 cut(s)	84, 164, 567, 1239
SalI	GTCGAC	2 cut(s)	29, 1313
SaqAI	TTAA	1 cut(s)	276
SatI	GCNGC	8 cut(s)	366, 486, 489, 1002, 1086, 1149, 1353, 1356
Sau3AI	GATC	3 cut(s)	211, 889, 1224
Sau96I	GGNCC	2 cut(s)	461, 509
SchI	GAGTC	3 cut(s)	329, 788, 1310
ScrFI	CCNGG	5 cut(s)	829, 864, 887, 1268, 1290
SduI	GDGCHC	2 cut(s)	437, 1236
SfaNI	GCATC	6 cut(s)	279, 505, 911, 922, 998, 1339
SfcI	CTRYAG	1 cut(s)	363
SinI	GGWCC	2 cut(s)	461, 509
SmiMI	CAYNNNNRTG	4 cut(s)	84, 164, 567, 1239
SmlI	CTYRAG	1 cut(s)	194
SmoI	CTYRAG	1 cut(s)	194
SphI	GCATGC	1 cut(s)	351
Sse9I	AATT	9 cut(s)	18, 207, 222, 294, 385, 540, 668, 689, 704
SsiI	CCGC	3 cut(s)	446, 1051, 1353
SspMI	CTAG	1 cut(s)	594
StyD4I	CCNGG	5 cut(s)	827, 862, 885, 1266, 1288
StyI	CCWWGG	1 cut(s)	69
TaaI	ACNGT	6 cut(s)	178, 253, 380, 443, 1062, 1391
TaiI	ACGT	1 cut(s)	15
TaqI	TCGA	3 cut(s)	30, 234, 1314
TasI	AATT	9 cut(s)	18, 207, 222, 294, 385, 540, 668, 689, 704
TatI	WGTACW	2 cut(s)	102, 981
TauI	GCSGC	1 cut(s)	1355
TfiI	GAWTC	2 cut(s)	653, 1332
Tru1I	TTAA	1 cut(s)	276
Tru9I	TTAA	1 cut(s)	276
TscAI	CASTG	4 cut(s)	183, 442, 448, 991
TseFI	GTSAC	2 cut(s)	1258, 1391
TseI	GCWGC	7 cut(s)	365, 485, 488, 1001, 1085, 1148, 1355
Tsp45I	GTSAC	2 cut(s)	1258, 1391
TspDTI	ATGAA	3 cut(s)	728, 1172, 1235
TspGWI	ACGGA	2 cut(s)	787, 1086
TspRI	CASTG	4 cut(s)	183, 442, 448, 991
VneI	GTGCAC	1 cut(s)	433
VpaK11BI	GGWCC	2 cut(s)	461, 509
XapI	RAATTY	1 cut(s)	18
XceI	RCATGY	3 cut(s)	351, 636, 982
XmiI	GTMKAC	2 cut(s)	30, 1314
XmnI	GAANNNNTTC	1 cut(s)	622
XspI	CTAG	1 cut(s)	594
Zsp2I	ATGCAT	1 cut(s)	349

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.