Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0007721

Physical Location & Seq

Genomic Coordinates Forward (+)

15394455 .. 15401203

Gene Structure 6749 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1088700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2484 bp

ATGTCTGGCCCTGCGGCCATTGGCGATAAAGCGGCAGAAGCACGATTGTTTACAGAGAGAACAAAGTCTCTTGAGGTCATTTCCAAAGAAGACAGAGAATCCATAATAAATAATGGTGAAATTGCTGATACTTCTGAAATTGCAGCCTTTAGAGTCAGGGAGCTATTACTTCCGAATAGGGAGTCCTATTCTGGACCCGTGCTTGGAAACATCCCTGAAGGGAAGGGAAAATATGTCTGGTCAGATGGCTGCATATATGAGGGTGAATGGAGACGGGGGATGAGACATGGGAATGGAAAAATTATTTGGTCTTCTGGTGGTGTTTATGAGGGCGAATTTTTAGGTGGATATATGCATGGAACAGGGACCTACATTGGGTCGGATAAGATGACTTATAAGGGGCGATGGCGGTTGAATCTCAAACATGGTTTGGGATATCAAGTCTATCCTAGTGGAGATGCCTTTGAAGGTTCTTGGATGCAGGGGTTGGCAGAAGGGCCTGGCAAGTATACGTGGGTAAATGGAAATGTTTATCTAGGAAATATGAAAGGAGGGAAAATGTCAGGGAAAGGAACTCTCACATGGACCAATGGGGACTCATTTGAAGGAAGCTGGTTGAATGGCATGATGCATGGATTTGGAGTATACACGTGGAGCGATGGAGGCATCTATGTTGGAACTTGGACCCGCGGTTTAAAAGATGGGAAAGGGTCATTTTATCCAAAAGGCAGCAGACTTCCAGTAGTAGAAGAACTTTACATGAACGCTTTGAGGAGACGAGGGCTCTTGCCAGATTTAAGAAAACAGAACAACGCACATATCCATAATGCTGGTTCAGGGGATATAGGAAATGTGAAGATTGGAGAGAGCCTGCAGGGATCTCATCGTGCTTCAGCCGATAAAATCTCTAATAGAAATCTGTTAAATTTGGAGCAGTCACATAGAAATGTTTCCTTGGAAAGGCGTTGGAGTATGGAGGTATCTTTCGAAAAAGTTATTGGCCATGATCTATCATTAGCTGAGGGTGGAGAAAAAGATTCTGAGACAGGAATTCCAATCTTAGAACGAGAATATATGCAGGGTGTCTTAATCAGTGAGCTAGTAATGAATAACAGCTTTTCTTCGTCATCTAGAAGGGCAAAACGGCGACAAAAGAAGCTAGCAAAAGTGGTGAAGAGACCTGGTGAAGCGATCATTAAAGGTCATAGGAGCTATGATCTAATGCTCAGTTTGCAGCTGGGTATCAGGTATACTGTTGGGAAGATAACACCAATACAAAGGAGAGAAGTCCGGGCATCAGATTTTGGGCCTCGAGCAAGTTTTTGGATGAATTTTCCTAAAGAAGGCTCCCAATTGACACCGCCTCATCAATCAGTAGATTTTAGGTGGAAAGACTATTGCCCCATGGTCTTTAGGAATTTGAGGGAGATGTTCAAGATTGATGCTGCTGACTACATGATGTCTATTTGTGGAAATGATGCTCTCAGAGAACTTTCTTCTCCCGGAAAAAGTGGTAGTGTGTTTTTCCTATCTCAAGATGACCGTTTCATGATTAAGACACTCAGGAAATCTGAAGTTCAGGTTCTTCTGCGGATGCTTCCTAGTTATCATCATCATGTAAGGTCGTATGAGAACACCCTCATCACAAAATTCTTTGGACTTCATAGGATTAAACCTTCAAGTGGTCAAAAGTTCCGCTTTGTAGTGATGGGAAACATGTTCTGCACAGAGCTAAGAATACATCGAAGATTTGATTTGAAAGGTTCATCCCTTGGTCGTTCTGCTGATAAGATTGAAATCGACGAAAACACAACGCTCAAGGACTTGGATTTAACCTACTCTTTTTATCTCGAACCTTCTTGGCGAGAGGCCTTAATGACTCAGAGTGAGATCGACAGTAAATTTCTGGAGGCGCAGCACATTATGGACTACAGCCTTCTCATGGGAGTGCATTATCGAGCCCCCCAACATCTGCGGTCTATCACGTCCTACAATAATACCACTCGAGCTGATGGTTTGGGAATCCTTGCAGAGGAAGAGACTCTGGAGGACGAGATCTCCAATTACCCACAGGGACTTGTCTTAATCCCACGTGGAAGCAACGACGCTAGTGTTGTTGTTGGCCCTCATGTGAGAGGTAGCCGACTGAGAGCATCATCTGCCGGTTATGAGGAAGTCGATCTCCTATTACCCGGTACAGCAAGGCTCCAAATCCAGCTGGGTGTGAACATGCCAGCAAGGGCAGAGCAGGTTCCCGGCGAACAGGGAGACCAGCAAACATTTCATGAAGTATACGACGTTGTGCTATATCTTGGGATCATTGATATATTGCAAGACTACAGCATGGGAAAGAAGATTGAACACGCGTACAAATCTCTTCAATTCGATTCCTTGTCCATCTCCGCTGTGGACCCGACATTCTATTCCCGGCGCTTCTTGGAATTCATCCGGAAGGTCTTCCCTCCAAATGCCTTAACAAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006520 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04011 ko04070 ko04072 ko04139 ko04144 ko04666 ko04810 ko05231 map00562 map01100 map04011 map04070 map04072 map04139 map04144 map04666 map04810 map05231

Pfam Domains

Protein Families

MORN PIP5K

Protein Analysis

828

Amino Acids

93.26

Weight (kDa)

9.04

Isoelectric Point (pI)

45.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MORN	PF02493	86 - 108	2.4e-07	MORN repeat
MORN	PF02493	155 - 177	5.8e-06	MORN repeat
MORN	PF02493	201 - 222	4.8e-08	MORN repeat
PIP5K	PF01504	489 - 818	3.1e-98	Phosphatidylinositol-4-phosphate 5-Kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007721)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09920 AT3G09920 AT3G09920 AT3G09920
fragaria_vesca	FvH4_6g16890 FvH4_6g16890 FvH4_6g16890
malus_domestica	MD04G1088700.v1.1 MD12G1106400.v1.1
prunus_persica	Prupe.7G125200_v2.0.a1 Prupe.7G125200_v2.0.a1
pyrus_communis	pycom04g08300 pycom12g10690
rosa_chinensis	RchiOBHm_Chr3g0470911
rosa_laevigata	RLG00000024234
rosa_multiflora	Rmu_co8399353.1_g000001 Rmu_sc0003361.1_g000002 Rmu_sc0010267.1_g000001
rosa_roxburghii	Rroxscaffold_6G00410420
rosa_rugosa	Rorug03G0113600
rosa_samantha	Rh3BG188800 Rh3CG178800 Rh3DG187300
rosa_wichuraiana	Rw3G015390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	396
Acc36I	ACCTGC	1 cut(s)	2239
AccI	GTMKAC	4 cut(s)	509, 645, 1250, 2292
AccII	CGCG	2 cut(s)	690, 2366
AccIII	TCCGGA	1 cut(s)	2448
AclWI	GGATC	2 cut(s)	886, 2324
AcoI	YGGCCR	2 cut(s)	15, 1000
AcsI	RAATTY	8 cut(s)	335, 925, 1050, 1330, 1417, 1649, 1901, 2441
AcuI	CTGAAG	3 cut(s)	237, 876, 1593
AcvI	CACGTG	2 cut(s)	651, 2093
AfaI	GTAC	2 cut(s)	2197, 2369
AfiI	CCNNNNNNNGG	8 cut(s)	203, 375, 960, 1343, 1682, 1942, 2032, 2478
AflIII	ACRYGT	3 cut(s)	648, 1716, 2364
AjiI	CACGTC	1 cut(s)	1986
AjnI	CCWGG	2 cut(s)	499, 1180
AjuI	GAANNNNNNNTTGG	4 cut(s)	289, 321, 981, 1013
Alw26I	GTCTC	8 cut(s)	72, 265, 277, 769, 1037, 1171, 2033, 2262
AlwI	GGATC	2 cut(s)	886, 2324
Ama87I	CYCGRG	2 cut(s)	1311, 2004
Aor13HI	TCCGGA	1 cut(s)	2448
AoxI	GGCC	7 cut(s)	7, 15, 497, 1000, 1307, 1869, 2122
ApeKI	GCWGC	6 cut(s)	143, 249, 729, 1234, 1445, 1915
ApoI	RAATTY	8 cut(s)	335, 925, 1050, 1330, 1417, 1649, 1901, 2441
ArsI	GACNNNNNNTTYG	2 cut(s)	167, 199
Asp700I	GAANNNNTTC	2 cut(s)	949, 2456
AspLEI	GCGC	2 cut(s)	1915, 2433
AspS9I	GGNCC	9 cut(s)	8, 194, 366, 497, 585, 684, 1307, 2123, 2410
AsuC2I	CCSGG	5 cut(s)	1292, 1503, 2193, 2256, 2428
AsuHPI	GGTGA	4 cut(s)	128, 275, 1183, 1196
AsuII	TTCGAA	1 cut(s)	987
AsuNHI	GCTAGC	1 cut(s)	1159
AvaI	CYCGRG	2 cut(s)	1311, 2004
AvaII	GGWCC	5 cut(s)	194, 366, 585, 684, 2410
BalI	TGGCCA	1 cut(s)	1002
BanII	GRGCYC	2 cut(s)	786, 1963
BbrPI	CACGTG	2 cut(s)	651, 2093
BbsI	GAAGAC	3 cut(s)	96, 303, 2449
BbvCI	CCTCAGC	1 cut(s)	1020
BbvI	GCAGC	6 cut(s)	155, 236, 741, 1246, 1432, 1927
BccI	CCATC	7 cut(s)	239, 399, 653, 695, 1702, 2006, 2405
BceAI	ACGGC	1 cut(s)	1160
BciT130I	CCWGG	2 cut(s)	501, 1182
BcnI	CCSGG	5 cut(s)	1292, 1503, 2193, 2256, 2428
BcoDI	GTCTC	8 cut(s)	72, 265, 277, 769, 1037, 1171, 2033, 2262
BfaI	CTAG	7 cut(s)	450, 536, 1100, 1131, 1160, 1602, 2109
BfmI	CTRYAG	3 cut(s)	872, 1930, 2338
BfoI	RGCGCY	1 cut(s)	2434
BfuAI	ACCTGC	1 cut(s)	2239
BglII	AGATCT	1 cut(s)	2055
BisI	GCNGC	8 cut(s)	15, 33, 144, 250, 730, 1235, 1446, 1916
BlsI	GCNGC	8 cut(s)	16, 34, 145, 251, 731, 1236, 1447, 1917
Bme1390I	CCNGG	7 cut(s)	501, 1182, 1292, 1503, 2193, 2256, 2428
Bme18I	GGWCC	5 cut(s)	194, 366, 585, 684, 2410
BmeT110I	CYCGRG	2 cut(s)	1311, 2004
BmgBI	CACGTC	1 cut(s)	1986
BmgT120I	GGNCC	9 cut(s)	8, 194, 366, 497, 585, 684, 1307, 2123, 2410
BmiI	GGNNCC	7 cut(s)	196, 367, 686, 1348, 2207, 2253, 2412
BmrFI	CCNGG	7 cut(s)	501, 1182, 1292, 1503, 2193, 2256, 2428
BmsI	GCATC	9 cut(s)	448, 468, 618, 675, 1304, 1432, 1468, 1584, 2162
BmtI	GCTAGC	1 cut(s)	1163
BpiI	GAAGAC	3 cut(s)	96, 303, 2449
BpmI	CTGGAG	2 cut(s)	1928, 2066
Bpu10I	CCTNAGC	1 cut(s)	1020
Bpu14I	TTCGAA	1 cut(s)	987
BpuEI	CTTGAG	3 cut(s)	92, 1518, 1802
BpuMI	CCSGG	5 cut(s)	1292, 1503, 2193, 2256, 2428
BsaAI	YACGTR	3 cut(s)	513, 651, 2093
BsaBI	GATNNNNATC	1 cut(s)	1796
BsaI	GGTCTC	2 cut(s)	1171, 2262
BsaJI	CCNNGG	4 cut(s)	688, 954, 1404, 1771
BsaWI	WCCGGW	1 cut(s)	2448
BsaXI	ACNNNNNCTCC	2 cut(s)	2042, 2072
Bsc4I	CCNNNNNNNGG	8 cut(s)	203, 375, 960, 1343, 1682, 1942, 2032, 2478
Bse118I	RCCGGY	1 cut(s)	2162
Bse1I	ACTGG	1 cut(s)	740
Bse8I	GATNNNNATC	1 cut(s)	1796
BseAI	TCCGGA	1 cut(s)	2448
BseBI	CCWGG	2 cut(s)	501, 1182
BseDI	CCNNGG	4 cut(s)	688, 954, 1404, 1771
BseGI	GGATG	7 cut(s)	210, 285, 483, 1332, 1599, 1766, 2445
BseJI	GATNNNNATC	1 cut(s)	1796
BseLI	CCNNNNNNNGG	8 cut(s)	203, 375, 960, 1343, 1682, 1942, 2032, 2478
BseMII	CTCAG	7 cut(s)	1011, 1032, 1240, 1498, 1576, 1895, 2138
BseNI	ACTGG	1 cut(s)	740
BseRI	GAGGAG	1 cut(s)	787
BseXI	GCAGC	6 cut(s)	155, 236, 741, 1246, 1432, 1927
BseYI	CCCAGC	2 cut(s)	1237, 2218
BsgI	GTGCAG	1 cut(s)	1708
Bsh1236I	CGCG	2 cut(s)	690, 2366
BshFI	GGCC	7 cut(s)	9, 17, 499, 1002, 1309, 1871, 2124
BsiHKCI	CYCGRG	2 cut(s)	1311, 2004
BsiSI	CCGG	7 cut(s)	1291, 1503, 2163, 2193, 2256, 2428, 2449
BslFI	GGGAC	3 cut(s)	379, 608, 2088
BslI	CCNNNNNNNGG	8 cut(s)	203, 375, 960, 1343, 1682, 1942, 2032, 2478
BsmAI	GTCTC	8 cut(s)	72, 265, 277, 769, 1037, 1171, 2033, 2262
BsmBI	CGTCTC	2 cut(s)	265, 769
BsmFI	GGGAC	3 cut(s)	379, 608, 2088
BsnI	GGCC	7 cut(s)	9, 17, 499, 1002, 1309, 1871, 2124
Bso31I	GGTCTC	2 cut(s)	1171, 2262
BsoBI	CYCGRG	2 cut(s)	1311, 2004
Bsp119I	TTCGAA	1 cut(s)	987
Bsp1286I	GDGCHC	2 cut(s)	786, 1963
Bsp13I	TCCGGA	1 cut(s)	2448
Bsp143I	GATC	8 cut(s)	878, 1006, 1191, 1216, 1890, 2055, 2179, 2316
Bsp19I	CCATGG	1 cut(s)	1404
BspANI	GGCC	7 cut(s)	9, 17, 499, 1002, 1309, 1871, 2124
BspCNI	CTCAG	7 cut(s)	1012, 1033, 1239, 1497, 1575, 1894, 2139
BspEI	TCCGGA	1 cut(s)	2448
BspFNI	CGCG	2 cut(s)	690, 2366
BspHI	TCATGA	2 cut(s)	1548, 2284
BspLI	GGNNCC	7 cut(s)	196, 367, 686, 1348, 2207, 2253, 2412
BspMAI	CTGCAG	1 cut(s)	876
BspMI	ACCTGC	1 cut(s)	2239
BspOI	GCTAGC	1 cut(s)	1163
BspPI	GGATC	2 cut(s)	886, 2324
BspT104I	TTCGAA	1 cut(s)	987
BspTNI	GGTCTC	2 cut(s)	1171, 2262
BsrFI	RCCGGY	1 cut(s)	2162
BsrI	ACTGG	1 cut(s)	740
BssAI	RCCGGY	1 cut(s)	2162
BssECI	CCNNGG	4 cut(s)	688, 954, 1404, 1771
BssMI	GATC	8 cut(s)	878, 1006, 1191, 1216, 1890, 2055, 2179, 2316
BssNAI	GTATAC	4 cut(s)	510, 646, 1251, 2293
BssT1I	CCWWGG	3 cut(s)	954, 1404, 1771
Bst1107I	GTATAC	4 cut(s)	510, 646, 1251, 2293
Bst2UI	CCWGG	2 cut(s)	501, 1182
Bst4CI	ACNGT	3 cut(s)	1255, 1544, 1898
Bst6I	CTCTTC	3 cut(s)	1169, 2031, 2382
BstAPI	GCANNNNNTGC	1 cut(s)	2159
BstBAI	YACGTR	3 cut(s)	513, 651, 2093
BstBI	TTCGAA	1 cut(s)	987
BstC8I	GCNNGC	3 cut(s)	872, 1161, 2235
BstDEI	CTNAG	9 cut(s)	1020, 1041, 1060, 1226, 1484, 1562, 1733, 1881, 2147
BstDSI	CCRYGG	2 cut(s)	688, 1404
BstENI	CCTNNNNNAGG	4 cut(s)	958, 1341, 2030, 2476
BstF5I	GGATG	7 cut(s)	210, 285, 483, 1332, 1599, 1766, 2445
BstFNI	CGCG	2 cut(s)	690, 2366
BstH2I	RGCGCY	1 cut(s)	2434
BstHHI	GCGC	2 cut(s)	1915, 2433
BstKTI	GATC	8 cut(s)	881, 1009, 1194, 1219, 1893, 2058, 2182, 2319
BstMAI	GTCTC	8 cut(s)	72, 265, 277, 769, 1037, 1171, 2033, 2262
BstMBI	GATC	8 cut(s)	878, 1006, 1191, 1216, 1890, 2055, 2179, 2316
BstMWI	GCNNNNNNNGC	4 cut(s)	38, 663, 1231, 2159
BstNI	CCWGG	2 cut(s)	501, 1182
BstNSI	RCATGY	2 cut(s)	1720, 2233
BstSCI	CCNGG	7 cut(s)	499, 1180, 1290, 1501, 2191, 2254, 2426
BstSFI	CTRYAG	3 cut(s)	872, 1930, 2338
BstUI	CGCG	2 cut(s)	690, 2366
BstV1I	GCAGC	6 cut(s)	155, 236, 741, 1246, 1432, 1927
BstV2I	GAAGAC	3 cut(s)	96, 303, 2449
BstX2I	RGATCY	2 cut(s)	878, 2055
BstXI	CCANNNNNNTGG	1 cut(s)	830
BstYI	RGATCY	2 cut(s)	878, 2055
BstZ17I	GTATAC	4 cut(s)	510, 646, 1251, 2293
BsuRI	GGCC	7 cut(s)	9, 17, 499, 1002, 1309, 1871, 2124
BtgI	CCRYGG	2 cut(s)	688, 1404
BtgZI	GCGATG	2 cut(s)	418, 672
BtrI	CACGTC	1 cut(s)	1986
BtsCI	GGATG	7 cut(s)	210, 285, 483, 1332, 1599, 1766, 2445
BtsIMutI	CAGTG	1 cut(s)	1099
BveI	ACCTGC	1 cut(s)	2239
Cac8I	GCNNGC	3 cut(s)	872, 1161, 2235
CciI	TCATGA	2 cut(s)	1548, 2284
CfoI	GCGC	2 cut(s)	1915, 2433
Cfr10I	RCCGGY	1 cut(s)	2162
Cfr13I	GGNCC	9 cut(s)	8, 194, 366, 497, 585, 684, 1307, 2123, 2410
Cfr42I	CCGCGG	1 cut(s)	691
CseI	GACGC	1 cut(s)	2114
CsiI	ACCWGGT	1 cut(s)	1180
Csp6I	GTAC	2 cut(s)	2196, 2368
CviQI	GTAC	2 cut(s)	2196, 2368
DdeI	CTNAG	9 cut(s)	1020, 1041, 1060, 1226, 1484, 1562, 1733, 1881, 2147
DpnI	GATC	8 cut(s)	880, 1008, 1193, 1218, 1892, 2057, 2181, 2318
DpnII	GATC	8 cut(s)	878, 1006, 1191, 1216, 1890, 2055, 2179, 2316
DraI	TTTAAA	1 cut(s)	696
EaeI	YGGCCR	2 cut(s)	15, 1000
Eam1104I	CTCTTC	3 cut(s)	1169, 2031, 2382
EarI	CTCTTC	3 cut(s)	1169, 2031, 2382
Eco130I	CCWWGG	3 cut(s)	954, 1404, 1771
Eco147I	AGGCCT	1 cut(s)	1871
Eco24I	GRGCYC	2 cut(s)	786, 1963
Eco31I	GGTCTC	2 cut(s)	1171, 2262
Eco32I	GATATC	1 cut(s)	437
Eco47I	GGWCC	5 cut(s)	194, 366, 585, 684, 2410
Eco57I	CTGAAG	3 cut(s)	237, 876, 1593
Eco72I	CACGTG	2 cut(s)	651, 2093
Eco88I	CYCGRG	2 cut(s)	1311, 2004
EcoNI	CCTNNNNNAGG	4 cut(s)	958, 1341, 2030, 2476
EcoO109I	RGGNCCY	2 cut(s)	366, 497
EcoRI	GAATTC	2 cut(s)	1050, 2441
EcoRII	CCWGG	2 cut(s)	499, 1180
EcoRV	GATATC	1 cut(s)	437
EcoT14I	CCWWGG	3 cut(s)	954, 1404, 1771
EcoT22I	ATGCAT	2 cut(s)	357, 633
EcoT38I	GRGCYC	2 cut(s)	786, 1963
ErhI	CCWWGG	3 cut(s)	954, 1404, 1771
Esp3I	CGTCTC	2 cut(s)	265, 769
FalI	AAGNNNNNCTT	2 cut(s)	1682, 1714
FaqI	GGGAC	3 cut(s)	379, 608, 2088
FauI	CCCGC	1 cut(s)	695
FblI	GTMKAC	4 cut(s)	509, 645, 1250, 2292
Fnu4HI	GCNGC	8 cut(s)	15, 33, 144, 250, 730, 1235, 1446, 1916
FokI	GGATG	7 cut(s)	197, 292, 490, 1339, 1606, 1753, 2432
FriOI	GRGCYC	2 cut(s)	786, 1963
Fsp4HI	GCNGC	8 cut(s)	15, 33, 144, 250, 730, 1235, 1446, 1916
FspBI	CTAG	7 cut(s)	450, 536, 1100, 1131, 1160, 1602, 2109
GlaI	GCGC	2 cut(s)	1914, 2432
GluI	GCNGC	8 cut(s)	15, 33, 144, 250, 730, 1235, 1446, 1916
GsaI	CCCAGC	2 cut(s)	1241, 2222
GsuI	CTGGAG	2 cut(s)	1928, 2066
HaeII	RGCGCY	1 cut(s)	2434
HaeIII	GGCC	7 cut(s)	9, 17, 499, 1002, 1309, 1871, 2124
HapII	CCGG	7 cut(s)	1291, 1503, 2163, 2193, 2256, 2428, 2449
HgaI	GACGC	1 cut(s)	2114
HhaI	GCGC	2 cut(s)	1915, 2433
Hin6I	GCGC	2 cut(s)	1913, 2431
HinP1I	GCGC	2 cut(s)	1913, 2431
HpaII	CCGG	7 cut(s)	1291, 1503, 2163, 2193, 2256, 2428, 2449
HphI	GGTGA	4 cut(s)	128, 275, 1183, 1196
Hpy166II	GTNNAC	7 cut(s)	51, 510, 646, 1251, 2227, 2293, 2410
Hpy188I	TCNGA	9 cut(s)	136, 174, 244, 382, 1042, 1300, 1487, 1573, 1884
Hpy8I	GTNNAC	7 cut(s)	51, 510, 646, 1251, 2227, 2293, 2410
Hpy99I	CGWCG	3 cut(s)	1805, 2108, 2300
HpyCH4III	ACNGT	3 cut(s)	1255, 1544, 1898
HpyCH4IV	ACGT	5 cut(s)	512, 650, 1985, 2092, 2298
HpyF10VI	GCNNNNNNNGC	4 cut(s)	38, 663, 1231, 2159
HpyF3I	CTNAG	9 cut(s)	1020, 1041, 1060, 1226, 1484, 1562, 1733, 1881, 2147
HpySE526I	ACGT	5 cut(s)	512, 650, 1985, 2092, 2298
HspAI	GCGC	2 cut(s)	1913, 2431
Kpn2I	TCCGGA	1 cut(s)	2448
KspI	CCGCGG	1 cut(s)	691
Kzo9I	GATC	8 cut(s)	878, 1006, 1191, 1216, 1890, 2055, 2179, 2316
LmnI	GCTCC	6 cut(s)	160, 654, 931, 1209, 1352, 2211
Lsp1109I	GCAGC	6 cut(s)	155, 236, 741, 1246, 1432, 1927
LweI	GCATC	9 cut(s)	448, 468, 618, 675, 1304, 1432, 1468, 1584, 2162
MabI	ACCWGGT	1 cut(s)	1180
MaeI	CTAG	7 cut(s)	450, 536, 1100, 1131, 1160, 1602, 2109
MaeII	ACGT	5 cut(s)	512, 650, 1985, 2092, 2298
MaeIII	GTNAC	1 cut(s)	936
MalI	GATC	8 cut(s)	880, 1008, 1193, 1218, 1892, 2057, 2181, 2318
MboI	GATC	8 cut(s)	878, 1006, 1191, 1216, 1890, 2055, 2179, 2316
MfeI	CAATTG	1 cut(s)	1352
MflI	RGATCY	2 cut(s)	878, 2055
MhlI	GDGCHC	2 cut(s)	786, 1963
MlsI	TGGCCA	1 cut(s)	1002
MluI	ACGCGT	1 cut(s)	2364
MluNI	TGGCCA	1 cut(s)	1002
MlyI	GAGTC	5 cut(s)	162, 191, 590, 1873, 2035
MmeI	TCCRAC	3 cut(s)	360, 655, 947
Mox20I	TGGCCA	1 cut(s)	1002
Mph1103I	ATGCAT	2 cut(s)	357, 633
MroI	TCCGGA	1 cut(s)	2448
MroXI	GAANNNNTTC	2 cut(s)	949, 2456
MscI	TGGCCA	1 cut(s)	1002
MslI	CAYNNNNRTG	4 cut(s)	291, 945, 1614, 1946
Msp20I	TGGCCA	1 cut(s)	1002
MspA1I	CMGCKG	4 cut(s)	690, 1237, 2218, 2405
MspI	CCGG	7 cut(s)	1291, 1503, 2163, 2193, 2256, 2428, 2449
MspR9I	CCNGG	7 cut(s)	501, 1182, 1292, 1503, 2193, 2256, 2428
MunI	CAATTG	1 cut(s)	1352
MvaI	CCWGG	2 cut(s)	501, 1182
MvnI	CGCG	2 cut(s)	690, 2366
MwoI	GCNNNNNNNGC	4 cut(s)	38, 663, 1231, 2159
NciI	CCSGG	5 cut(s)	1292, 1503, 2193, 2256, 2428
NcoI	CCATGG	1 cut(s)	1404
NdeII	GATC	8 cut(s)	878, 1006, 1191, 1216, 1890, 2055, 2179, 2316
NheI	GCTAGC	1 cut(s)	1159
NlaIV	GGNNCC	7 cut(s)	196, 367, 686, 1348, 2207, 2253, 2412
NmuCI	GTSAC	1 cut(s)	936
NsiI	ATGCAT	2 cut(s)	357, 633
NspI	RCATGY	2 cut(s)	1720, 2233
NspV	TTCGAA	1 cut(s)	987
PaeR7I	CTCGAG	2 cut(s)	1311, 2004
PagI	TCATGA	2 cut(s)	1548, 2284
PceI	AGGCCT	1 cut(s)	1871
PciI	ACATGT	1 cut(s)	1716
PdmI	GAANNNNTTC	2 cut(s)	949, 2456
PfeI	GAWTC	5 cut(s)	98, 415, 1037, 2022, 2387
PfoI	TCCNGGA	1 cut(s)	1501
PkrI	GCNGC	8 cut(s)	16, 34, 145, 251, 731, 1236, 1447, 1917
PleI	GAGTC	5 cut(s)	161, 190, 590, 1873, 2035
PmaCI	CACGTG	2 cut(s)	651, 2093
PmlI	CACGTG	2 cut(s)	651, 2093
PpsI	GAGTC	5 cut(s)	161, 190, 590, 1873, 2035
Ppu21I	YACGTR	3 cut(s)	513, 651, 2093
PpuMI	RGGWCCY	1 cut(s)	366
PscI	ACATGT	1 cut(s)	1716
PsiI	TTATAA	1 cut(s)	396
Psp5II	RGGWCCY	1 cut(s)	366
Psp6I	CCWGG	2 cut(s)	499, 1180
PspCI	CACGTG	2 cut(s)	651, 2093
PspFI	CCCAGC	2 cut(s)	1237, 2218
PspGI	CCWGG	2 cut(s)	499, 1180
PspN4I	GGNNCC	7 cut(s)	196, 367, 686, 1348, 2207, 2253, 2412
PspPI	GGNCC	9 cut(s)	8, 194, 366, 497, 585, 684, 1307, 2123, 2410
PspPPI	RGGWCCY	1 cut(s)	366
PspXI	VCTCGAGB	2 cut(s)	1311, 2004
PstI	CTGCAG	1 cut(s)	876
PsuI	RGATCY	2 cut(s)	878, 2055
PvuII	CAGCTG	2 cut(s)	1237, 2218
RsaI	GTAC	2 cut(s)	2197, 2369
RsaNI	GTAC	2 cut(s)	2196, 2368
RseI	CAYNNNNRTG	4 cut(s)	291, 945, 1614, 1946
SacII	CCGCGG	1 cut(s)	691
SatI	GCNGC	8 cut(s)	15, 33, 144, 250, 730, 1235, 1446, 1916
Sau3AI	GATC	8 cut(s)	878, 1006, 1191, 1216, 1890, 2055, 2179, 2316
Sau96I	GGNCC	9 cut(s)	8, 194, 366, 497, 585, 684, 1307, 2123, 2410
SbfI	CCTGCAGG	1 cut(s)	876
SchI	GAGTC	5 cut(s)	162, 191, 590, 1873, 2035
ScrFI	CCNGG	7 cut(s)	501, 1182, 1292, 1503, 2193, 2256, 2428
SdaI	CCTGCAGG	1 cut(s)	876
SduI	GDGCHC	2 cut(s)	786, 1963
SexAI	ACCWGGT	1 cut(s)	1180
SfaNI	GCATC	9 cut(s)	448, 468, 618, 675, 1304, 1432, 1468, 1584, 2162
SfcI	CTRYAG	3 cut(s)	872, 1930, 2338
Sfr274I	CTCGAG	2 cut(s)	1311, 2004
Sfr303I	CCGCGG	1 cut(s)	691
SfuI	TTCGAA	1 cut(s)	987
SgrBI	CCGCGG	1 cut(s)	691
SinI	GGWCC	5 cut(s)	194, 366, 585, 684, 2410
SlaI	CTCGAG	2 cut(s)	1311, 2004
SmiMI	CAYNNNNRTG	4 cut(s)	291, 945, 1614, 1946
SmlI	CTYRAG	5 cut(s)	71, 1311, 1533, 1817, 2004
SmoI	CTYRAG	5 cut(s)	71, 1311, 1533, 1817, 2004
Sse8387I	CCTGCAGG	1 cut(s)	876
SseBI	AGGCCT	1 cut(s)	1871
SspMI	CTAG	7 cut(s)	450, 536, 1100, 1131, 1160, 1602, 2109
StuI	AGGCCT	1 cut(s)	1871
StyD4I	CCNGG	7 cut(s)	499, 1180, 1290, 1501, 2191, 2254, 2426
StyI	CCWWGG	3 cut(s)	954, 1404, 1771
TaaI	ACNGT	3 cut(s)	1255, 1544, 1898
TaiI	ACGT	5 cut(s)	515, 653, 1988, 2095, 2301
TauI	GCSGC	2 cut(s)	17, 35
TfiI	GAWTC	5 cut(s)	98, 415, 1037, 2022, 2387
TscAI	CASTG	1 cut(s)	1099
TseFI	GTSAC	1 cut(s)	936
TseI	GCWGC	6 cut(s)	143, 249, 729, 1234, 1445, 1915
Tsp45I	GTSAC	1 cut(s)	936
TspRI	CASTG	1 cut(s)	1099
VpaK11BI	GGWCC	5 cut(s)	194, 366, 585, 684, 2410
XagI	CCTNNNNNAGG	4 cut(s)	958, 1341, 2030, 2476
XapI	RAATTY	8 cut(s)	335, 925, 1050, 1330, 1417, 1649, 1901, 2441
XbaI	TCTAGA	1 cut(s)	1130
XceI	RCATGY	2 cut(s)	1720, 2233
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2404
XhoI	CTCGAG	2 cut(s)	1311, 2004
XmiI	GTMKAC	4 cut(s)	509, 645, 1250, 2292
XmnI	GAANNNNTTC	2 cut(s)	949, 2456
XspI	CTAG	7 cut(s)	450, 536, 1100, 1131, 1160, 1602, 2109
Zsp2I	ATGCAT	2 cut(s)	357, 633

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.