pycom04g08300

phosphatidylinositol 4-phosphate 5-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007721
Physical Location & Seq
Forward (+)
9910325 .. 9911053
729 bp
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UTR
Exon/CDS
Intron
pycom04g08300.1

Sequence Viewer

Length: 471 bp
ATGGTTTGGGAATCCTTGCAGAGGAAGACTCTGGAGGACGAGATCTCCAATTACCCACAGGGACTTGTCTTAATCCCTCGTGGAAGCGACGATGCTAGTGTTGTTGTTGGCCCCCATGTGAGAGGTAGCCGACTGAGAGCATCATCTGCCGGTTATGAGGAAGTCGATCTCCTATTACCCGGTACAGCAAGGCTCCAAATCCAGCTGGGTGTGAACATGCCAGCAAGGGCAGAGCAGGTTCCCGGTGAACAGGGAGACCAGCAAACATTTCATGATGTATACGACGTTGTGCTATATCTTGGGATCATTGATATATTGCAAGACTACAGCATGGGAAAGAAGATTGAACACGCGTACAAATCTCTTCAATTCGATTCCTTGTCCATCTCCGCTGTGGACCCGACATTCTATTCCCGGCGTTTCTTGGAATTCATCCGGAAGGTGTACCCTCCAAATGCCTTGACAAGGTAA

Protein Analysis

157

Amino Acids

17.57

Weight (kDa)

5.34

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIP5K PF01504 78 - 147 1.2e-21 Phosphatidylinositol-4-phosphate 5-Kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 226
AccI GTMKAC 1 cut(s) 279
AccII CGCG 1 cut(s) 353
AccIII TCCGGA 1 cut(s) 435
AciI CCGC 1 cut(s) 390
AclWI GGATC 1 cut(s) 311
AcsI RAATTY 1 cut(s) 428
AfaI GTAC 3 cut(s) 184, 356, 446
AfiI CCNNNNNNNGG 2 cut(s) 21, 465
AflIII ACRYGT 1 cut(s) 351
AgsI TTSAA 2 cut(s) 347, 368
AluBI AGCT 1 cut(s) 205
AluI AGCT 1 cut(s) 205
Alw26I GTCTC 1 cut(s) 249
AlwI GGATC 1 cut(s) 311
Aor13HI TCCGGA 1 cut(s) 435
AoxI GGCC 1 cut(s) 109
ApoI RAATTY 1 cut(s) 428
AspS9I GGNCC 2 cut(s) 110, 397
AsuC2I CCSGG 3 cut(s) 180, 243, 415
AsuHPI GGTGA 1 cut(s) 257
AvaII GGWCC 1 cut(s) 397
BauI CACGAG 1 cut(s) 78
BbsI GAAGAC 1 cut(s) 32
BccI CCATC 1 cut(s) 392
BcnI CCSGG 3 cut(s) 180, 243, 415
BcoDI GTCTC 1 cut(s) 249
BfaI CTAG 1 cut(s) 96
BfmI CTRYAG 1 cut(s) 325
BfuAI ACCTGC 1 cut(s) 226
BglII AGATCT 1 cut(s) 42
Bme1390I CCNGG 3 cut(s) 180, 243, 415
Bme18I GGWCC 1 cut(s) 397
BmgT120I GGNCC 2 cut(s) 110, 397
BmiI GGNNCC 4 cut(s) 112, 194, 240, 399
BmrFI CCNGG 3 cut(s) 180, 243, 415
BmsI GCATC 2 cut(s) 82, 149
BpiI GAAGAC 1 cut(s) 32
BplI GAGNNNNNCTC 2 cut(s) 13, 45
BpmI CTGGAG 1 cut(s) 53
BpuMI CCSGG 3 cut(s) 180, 243, 415
BsaI GGTCTC 1 cut(s) 249
BsaWI WCCGGW 1 cut(s) 435
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 465
Bse118I RCCGGY 1 cut(s) 149
BseAI TCCGGA 1 cut(s) 435
BseGI GGATG 1 cut(s) 432
BseLI CCNNNNNNNGG 2 cut(s) 21, 465
BseMII CTCAG 1 cut(s) 125
BseYI CCCAGC 1 cut(s) 205
Bsh1236I CGCG 1 cut(s) 353
BshFI GGCC 1 cut(s) 111
BsiSI CCGG 5 cut(s) 150, 180, 243, 415, 436
BslFI GGGAC 1 cut(s) 75
BslI CCNNNNNNNGG 2 cut(s) 21, 465
BsmAI GTCTC 1 cut(s) 249
BsmFI GGGAC 1 cut(s) 75
BsnI GGCC 1 cut(s) 111
Bso31I GGTCTC 1 cut(s) 249
Bsp13I TCCGGA 1 cut(s) 435
Bsp143I GATC 3 cut(s) 42, 166, 303
BspACI CCGC 1 cut(s) 390
BspANI GGCC 1 cut(s) 111
BspCNI CTCAG 1 cut(s) 126
BspEI TCCGGA 1 cut(s) 435
BspFNI CGCG 1 cut(s) 353
BspHI TCATGA 1 cut(s) 271
BspLI GGNNCC 4 cut(s) 112, 194, 240, 399
BspMI ACCTGC 1 cut(s) 226
BspPI GGATC 1 cut(s) 311
BspTNI GGTCTC 1 cut(s) 249
BsrFI RCCGGY 1 cut(s) 149
BssAI RCCGGY 1 cut(s) 149
BssMI GATC 3 cut(s) 42, 166, 303
BssNAI GTATAC 1 cut(s) 280
BssSI CACGAG 1 cut(s) 78
Bst1107I GTATAC 1 cut(s) 280
Bst2BI CACGAG 1 cut(s) 78
Bst6I CTCTTC 1 cut(s) 369
BstAPI GCANNNNNTGC 1 cut(s) 146
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 1 cut(s) 134
BstENI CCTNNNNNAGG 2 cut(s) 19, 463
BstF5I GGATG 1 cut(s) 432
BstFNI CGCG 1 cut(s) 353
BstKTI GATC 3 cut(s) 45, 169, 306
BstMAI GTCTC 1 cut(s) 249
BstMBI GATC 3 cut(s) 42, 166, 303
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNSI RCATGY 1 cut(s) 220
BstSCI CCNGG 3 cut(s) 178, 241, 413
BstSFI CTRYAG 1 cut(s) 325
BstUI CGCG 1 cut(s) 353
BstV2I GAAGAC 1 cut(s) 32
BstX2I RGATCY 1 cut(s) 42
BstYI RGATCY 1 cut(s) 42
BstZ17I GTATAC 1 cut(s) 280
BsuRI GGCC 1 cut(s) 111
BtsCI GGATG 1 cut(s) 432
BveI ACCTGC 1 cut(s) 226
Cac8I GCNNGC 1 cut(s) 222
CciI TCATGA 1 cut(s) 271
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 2 cut(s) 110, 397
Csp6I GTAC 3 cut(s) 183, 355, 445
CviAII CATG 4 cut(s) 116, 217, 272, 331
CviJI RGCY 4 cut(s) 111, 129, 193, 205
CviKI_1 RGCY 4 cut(s) 111, 129, 193, 205
CviQI GTAC 3 cut(s) 183, 355, 445
DdeI CTNAG 1 cut(s) 134
DpnI GATC 3 cut(s) 44, 168, 305
DpnII GATC 3 cut(s) 42, 166, 303
Eam1104I CTCTTC 1 cut(s) 369
EarI CTCTTC 1 cut(s) 369
Eco31I GGTCTC 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 397
EcoNI CCTNNNNNAGG 2 cut(s) 19, 463
EcoRI GAATTC 1 cut(s) 428
FaeI CATG 4 cut(s) 119, 220, 275, 334
FaiI YATR 8 cut(s) 117, 156, 218, 273, 280, 295, 314, 332
FaqI GGGAC 1 cut(s) 75
FatI CATG 4 cut(s) 115, 216, 271, 330
FblI GTMKAC 1 cut(s) 279
FokI GGATG 1 cut(s) 419
FspBI CTAG 1 cut(s) 96
GsaI CCCAGC 1 cut(s) 209
GsuI CTGGAG 1 cut(s) 53
HaeIII GGCC 1 cut(s) 111
HapII CCGG 5 cut(s) 150, 180, 243, 415, 436
Hin1II CATG 4 cut(s) 119, 220, 275, 334
HinfI GANTC 3 cut(s) 11, 28, 374
HpaII CCGG 5 cut(s) 150, 180, 243, 415, 436
HphI GGTGA 1 cut(s) 257
Hpy166II GTNNAC 5 cut(s) 214, 248, 280, 397, 445
Hpy188III TCNNGA 3 cut(s) 32, 272, 436
Hpy8I GTNNAC 5 cut(s) 214, 248, 280, 397, 445
Hpy99I CGWCG 2 cut(s) 92, 287
HpyAV CCTTC 1 cut(s) 433
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 2 cut(s) 19, 319
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 1 cut(s) 134
HpySE526I ACGT 1 cut(s) 285
Hsp92II CATG 4 cut(s) 119, 220, 275, 334
Kpn2I TCCGGA 1 cut(s) 435
Kzo9I GATC 3 cut(s) 42, 166, 303
LmnI GCTCC 1 cut(s) 198
LweI GCATC 2 cut(s) 82, 149
MaeI CTAG 1 cut(s) 96
MaeII ACGT 1 cut(s) 285
MalI GATC 3 cut(s) 44, 168, 305
MboI GATC 3 cut(s) 42, 166, 303
MboII GAAGA 3 cut(s) 37, 352, 356
MflI RGATCY 1 cut(s) 42
MluCI AATT 3 cut(s) 49, 368, 428
MluI ACGCGT 1 cut(s) 351
MlyI GAGTC 1 cut(s) 22
MnlI CCTC 6 cut(s) 15, 28, 87, 116, 151, 459
MroI TCCGGA 1 cut(s) 435
MseI TTAA 1 cut(s) 71
MspA1I CMGCKG 2 cut(s) 205, 392
MspI CCGG 5 cut(s) 150, 180, 243, 415, 436
MspR9I CCNGG 3 cut(s) 180, 243, 415
MvnI CGCG 1 cut(s) 353
MwoI GCNNNNNNNGC 1 cut(s) 146
NciI CCSGG 3 cut(s) 180, 243, 415
NdeII GATC 3 cut(s) 42, 166, 303
NlaIII CATG 4 cut(s) 119, 220, 275, 334
NlaIV GGNNCC 4 cut(s) 112, 194, 240, 399
NspI RCATGY 1 cut(s) 220
PagI TCATGA 1 cut(s) 271
PfeI GAWTC 2 cut(s) 11, 374
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
PspFI CCCAGC 1 cut(s) 205
PspN4I GGNNCC 4 cut(s) 112, 194, 240, 399
PspPI GGNCC 2 cut(s) 110, 397
PsuI RGATCY 1 cut(s) 42
PvuII CAGCTG 1 cut(s) 205
RsaI GTAC 3 cut(s) 184, 356, 446
RsaNI GTAC 3 cut(s) 183, 355, 445
SaqAI TTAA 1 cut(s) 71
Sau3AI GATC 3 cut(s) 42, 166, 303
Sau96I GGNCC 2 cut(s) 110, 397
SchI GAGTC 1 cut(s) 22
ScrFI CCNGG 3 cut(s) 180, 243, 415
SetI ASST 6 cut(s) 127, 207, 240, 288, 444, 470
SfaNI GCATC 2 cut(s) 82, 149
SfcI CTRYAG 1 cut(s) 325
SinI GGWCC 1 cut(s) 397
Sse9I AATT 3 cut(s) 49, 368, 428
SsiI CCGC 1 cut(s) 390
SspMI CTAG 1 cut(s) 96
StyD4I CCNGG 3 cut(s) 178, 241, 413
TaiI ACGT 1 cut(s) 288
TaqI TCGA 2 cut(s) 165, 372
TasI AATT 3 cut(s) 49, 368, 428
TfiI GAWTC 2 cut(s) 11, 374
Tru1I TTAA 1 cut(s) 71
Tru9I TTAA 1 cut(s) 71
TspDTI ATGAA 2 cut(s) 260, 421
VpaK11BI GGWCC 1 cut(s) 397
XagI CCTNNNNNAGG 2 cut(s) 19, 463
XapI RAATTY 1 cut(s) 428
XceI RCATGY 1 cut(s) 220
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 279
XspI CTAG 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.