Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0013415

Physical Location & Seq

Genomic Coordinates Forward (+)

16589437 .. 16595605

Gene Structure 6169 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1092800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4038 bp

ATGTCACAAGATTCTGAGGGGATAAAGACGATTGAGCAGTGGAGATGGTCCGAAATGCAAGGCCTTGAGCTGCTGCCGGATGCTGCCGATCCTTTTAAAACCAACCCAGCAGCAAACCCAGCAACCCCATCTAACCCATCAGCAACAACATCAACTCCCCAAGGGGCGGAGAGAATGGAGAGCTCCGAGCCCAGAAAGGACTCCACCGGCGGCGGCGAAAAGCCAGATTCTTTACCCGCAGTTCAGTTCAAAGAACTCTTCAGATTCGCTGATGGGTTGGATTACGTTCTGATGGCAATTGGATCAGTCGGAGCTCTCGTGCATGGCTGCTCTCTCCCCATTTTTCTCCGGTTCTTCGCCGATCTTGTTAATTCTTTTGGCTCGAATGCAAACGACATGGATAAGATGATGCACGAGGTTTTGAAGTACGCATTGTACTTTCTGGTGGTTGGAGCTGCCGTATGGGCGGGTTCATGGGCCGAGATTTCTTGTTGGATGTGGACCGGAGAGCGGCAGTCAACCAAGATGAGGATCAAGTACCTTGAGGCCGCTCTGAGCCAGGACATTCAGTACTTTGACACGGAGGTTCGAACTTCGGACGTCGTTTTCGCAATCAACACCGATACAGTGATGGTCCAGGACGCCATCAGTGAGAAGTTGGGTAATTTCATTCACTATATGGCAACATTTGTGTCTGGATTCGTGGTGGGTTTTACTGCTGTGTGGCAATTAGCACTTGTCACACTTGCTGTTGTTCCGCTGATAGCGGTGATCGGAGGCATTCATACCACCGCATTAGGCAAAATCTCAGGCAAGAGCCAAGAAGCTCTTTCACAAGCAGGACACACGGTGGAACAGACTGTAGCTCAAATTCGAGTTGTTTTGTCGTATGTTGGGGAATCGAGAGCGCTACAAGCATACTCCTCGGCATTGAAGGTGTCTCAGAAGCTTGGTTACAAAAGTGGATTTGCTAAAGGAATGGGACTCGGAGCGACTTACTTTGTAGTTTTCTGCTGTTATGCACTTCTTCTATGGTATGGAGGTTATCTTGTTAGGCACCACTTCACCAACGGCGGTCTTGCCATCGCTACCATGTTTGCTGTCATGCTAGGTGGAATAGGATTGGGACAGTCTGTCCCAAGCATGGGCGCATTTGTGAAGGCTAAAGTTGCAGCTGCGAAGATTTTCAAAATTATTGATCACAAACCGGGCATGGACCGCAATAGCGAAGCAGGAGTGGAATTAGAGTCTGTTACCGGACTAGTTGAGCTGAAAAATGTAGATTTTTCCTACCCTTCGAGGCAGGATGTTCGTATCCTCAACAATTTCTCGCTGAATGTTCCAGCTGGAAAGACCATTGCTTTGGTTGGTAGCAGTGGCTCTGGTAAGAGCACTGTGGTCTCCCTCATTGAGAGGTTTTATGATCCTTCCTCAGGACAAGTGCTGCTAGATGGACATGACATAAAGACATTGAAATTAAAGTGGCTGAGGCAGCAAATTGGGCTTGTGAGTCAAGAACCAGCTCTGTTTGCTACCACCATTAAAGAAAACATACTTTTGGGGCGGCCTGATGCCGACCAGGTTGAGATCGAAGAAGCTGCCAGAGTTGCCAATGCCCATTCATTTATTATCAAACTTCCTGACGGTTTTGATACTCAGGTAGGGGAGAGAGGACTACAATTGTCTGGAGGACAGAAGCAGAGAATAGCCATAGCAAGGGCAATGTTGAAAAACCCAGCAATCCTGCTATTAGACGAGGCAACGAGTGCATTGGACTCTGAGTCGGAAAAGCTGGTGCAAGAAGCCCTTGACCGGTTTATGATTGGAAGGACAACTCTTGTCATTGCCCATCGCCTATCAACCATTCGCAAAGCTGACCTTGTAGCTGTACTCCAACAAGGAAGTGTTTCTGAAATTGGAACCCATGATGAACTTTTCTTGAAAGGAGAAAACGGTGTCTATGCCAAGCTCATCCGGATGCAAGAGATGGCACACGAAACTGCTCTCAACAATGCGAGAAAGAGTAGCGCAAGGCCTTCTAGTGCCAGAAACTCTGTAAGCTCACCTATAATTGCCAGAAATTCCTCATATGGTCGATCACCATACTCACGCCGGCTATCGGACTTTTCTACTTCTGATTTTAGCTTCTCCCTTGATGCCTCATATCCCAATTACCGACTTGAAAAACTACCGTTCAAGGAGCAAGCTAGTTCCTTCTGGCGCCTTGCTAAAATGAACTCTCCTGAGTGGGTTTATGCTTTATTTGGTTCAGTTGGATCTGTTGTTTGTGGTTCTCTAAGTGCTTTCTTTTCATATGTTCTAAGTGCTGTCCTCAGTGTTTACTACAACCCAGACCATGCCTACATGATCAAACAAATTAATAAGTACTGTTACTTGTTAATTGGGCTTTCATCAGCTGCACTTATCTTCAACACATCACAACATTTCTTTTGGGATATTGTGGGAGAAAACCTCACAAAACGAGTGAGGGAGAAAATGCTGACAGCAGTTTTGAAAAATGAAATGGCGTGGTTTGATCAGGAGGAAAATGAGAGTGCCAGGATTTCAGCAAGGCTGGCTCTTGATGCTAACAATGTCAGATCAGCCATAGGAGACAGGATTTCTGTGATTGTACAGAACACAGCACTCATGCTTGTTGCTTGCACTGCGGGATTTGTTTTGCAATGGCGCCTTACCCTTGTCCTCATAGCAGTCTTTCCTTTTGTTGTTGCAGCCACAGTTTTGCAGAAATTGTTCATGACGGGTTTCTCGGGAGACCTTGAAGCAGCACATGCCAAGGCCACCCAACTAGCTGGGGAGGCCATAGCCAATGTAAGGACTGTTGCTGCATTCAATTCAGAGGGAAAAATTGTTGGTCTTTTCACCAGAAACCTCCAGATTCCACTACGCCGGTGCTTTTGGAAGGGCCAGATTGCTGGAAGTGGATTTGGGATAGCTCAGTTTGCACTTTATGCTTCCTATGCTCTAGGTCTTTGGTATGCTTCTTGGCTAGTGAAGCATGGAATCTCCGACTTCTCAAAGACAATTCGAGTTTTCGTGGTCCTCATGGTGTCTGCAAATGGTGCAGCAGAAACATTAACCCTGGCGCCTGACTTTATCAATGGTGGTCGAGCCATGCGATCAGTGTTTGAACTCCTTGACCGCAAAACTGAAATTGAACCTGATGATCCAGATGCTACTGTAGTCACTGATCGTCTGCGTGGAGAAATTGAATTGAAGCATGTAGACTTCACTTACCCTACTCGCCCTGATGTCCCTATTTTCCGTGACCTCTCCCTTCGTGCCCGAGCAGGCAAGACTCTTGCCCTTGTTGGTCCTAGTGGATGCGGTAAGAGTTCAGTCATTGCACTGATACAGCGATTCTACGACCCAACATCAGGACGAGTTCTGATTGATGGGAAGGATATTCGAAAATATAATCTGAAGTCCCTAAGAAGGCACATTGCCTTGGTTCCCCAGGAGCCATGCCTCTTTGCAACAACTATATACGAAAATATTGCTTATGGACACGAGCCAGCAACTGAGGCTGAGATCATTGAAGCTGCAAACCTGGCTAACGCTCACAAGTTCATATCTGCATTGCCGGAGGGTTACAAAACATTTGTTGGGGAACGGGGAGTTCAGTTATCTGGAGGACAGAAACAAAGAGTTGCAATTGCTAGGGCTTTATTGAGGAAGGCAGAGGTTATGCTTCTTGATGAGGCAACAAGTGCACTTGATGCTGAATCAGAGAGATCTATTCAAGAGGCACTAGAGCGTGCTTGCTCGGGAAAAACCACAATTGTGGTTGCACACAGACTATCGACCATCAGAAATGCACATGTCATTGCAGTGATTGATGATGGAAAGGTGGCAGAGCAAGGATCCCACTCCCATCTATTGAAAAATTACCCGGATGGGTGTTACGCGCGCATGATACAGCTACAAAGATTCACCCACACCCAAGCCGTTGGAATTGCATCAGGTTCAAGTTCATCAATAAAACCCCTAGAAGAGGAAGAAGGAGAAGGAAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006810 GO:0007275 GO:0008150 GO:0008361 GO:0009314 GO:0009411 GO:0009416 GO:0009506 GO:0009605 GO:0009606 GO:0009607 GO:0009624 GO:0009628 GO:0009629 GO:0009630 GO:0009637 GO:0009639 GO:0009640 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009908 GO:0009914 GO:0009926 GO:0009958 GO:0009966 GO:0009987 GO:0010033 GO:0010315 GO:0010328 GO:0010329 GO:0010646 GO:0010817 GO:0010928 GO:0015399 GO:0015405 GO:0015562 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022414 GO:0022804 GO:0022857 GO:0023051 GO:0030054 GO:0032501 GO:0032502 GO:0032535 GO:0042221 GO:0042623 GO:0042626 GO:0043207 GO:0043473 GO:0043476 GO:0043478 GO:0043479 GO:0043480 GO:0043481 GO:0043492 GO:0044464 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048583 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055044 GO:0055085 GO:0060918 GO:0060919 GO:0061458 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0080161 GO:0090066 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1346

Amino Acids

146.97

Weight (kDa)

8.04

Isoelectric Point (pI)

36.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	100 - 370	1.6e-52	ABC transporter transmembrane region
ABC_tran	PF00005	440 - 589	3.7e-35	ABC transporter
ABC_membrane	PF00664	757 - 1024	2.7e-50	ABC transporter transmembrane region
ABC_tran	PF00005	1096 - 1244	4.4e-35	ABC transporter
SMC_N	PF02463	1133 - 1284	8e-06	RecF/RecN/SMC N terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013415)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36910
fragaria_vesca	FvH4_6g16450 FvH4_6g16450 FvH4_6g16450
malus_domestica	MD04G1092800.v1.1
prunus_persica	Prupe.7G129600_v2.0.a1
pyrus_communis	pycom04g08730 pycom04g08740 pycom12g11150
rosa_chinensis	RchiOBHm_Chr3g0470311
rosa_laevigata	RLG00000024279
rosa_multiflora	Rmu_sc0001093.1_g000001
rosa_roxburghii	Rroxscaffold_6G00410950
rosa_rugosa	Rorug03G0108000
rosa_samantha	Rh3AG157800 Rh3BG182100 Rh3DG192800
rosa_wichuraiana	Rw3G015010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	603
AccB1I	GGYRCC	4 cut(s)	1056, 2220, 2688, 3106
AccBSI	CCGCTC	2 cut(s)	511, 551
AccI	GTMKAC	1 cut(s)	3246
AccII	CGCG	2 cut(s)	3929, 3931
AccIII	TCCGGA	1 cut(s)	1974
AclWI	GGATC	8 cut(s)	83, 310, 539, 1418, 2284, 3182, 3879, 3892
AcsI	RAATTY	2 cut(s)	870, 2080
AcuI	CTGAAG	2 cut(s)	244, 3464
AcyI	GRCGYC	5 cut(s)	600, 642, 2221, 2689, 3107
AdeI	CACNNNGTG	1 cut(s)	850
AfaI	GTAC	7 cut(s)	428, 437, 539, 572, 1890, 2387, 2634
AfeI	AGCGCT	1 cut(s)	909
AflIII	ACRYGT	1 cut(s)	3841
AgeI	ACCGGT	1 cut(s)	1812
AhdI	GACNNNNNGTC	2 cut(s)	1133, 1780
AhlI	ACTAGT	1 cut(s)	1261
AjnI	CCWGG	7 cut(s)	558, 636, 1578, 2558, 3102, 3477, 3570
AleI	CACNNNNGTG	1 cut(s)	3803
Alw21I	GWGCWC	4 cut(s)	185, 316, 1394, 3736
Alw26I	GTCTC	4 cut(s)	945, 1405, 2607, 2769
Alw44I	GTGCAC	1 cut(s)	3732
AlwI	GGATC	8 cut(s)	83, 310, 539, 1418, 2284, 3182, 3879, 3892
AlwNI	CAGNNNCTG	1 cut(s)	3542
Ama87I	CYCGRG	3 cut(s)	2770, 3306, 3787
Aor13HI	TCCGGA	1 cut(s)	1974
Aor51HI	AGCGCT	1 cut(s)	909
AoxI	GGCC	8 cut(s)	61, 477, 546, 1565, 2033, 2799, 2820, 2926
ApaLI	GTGCAC	1 cut(s)	3732
ApoI	RAATTY	2 cut(s)	870, 2080
ArsI	GACNNNNNNTTYG	6 cut(s)	590, 622, 1345, 1377, 2494, 2526
AseI	ATTAAT	1 cut(s)	2379
AsiGI	ACCGGT	1 cut(s)	1812
Asp700I	GAANNNNTTC	3 cut(s)	1184, 1906, 2753
AspLEI	GCGC	8 cut(s)	910, 1151, 2030, 2223, 2691, 3109, 3931, 3933
AspS9I	GGNCC	8 cut(s)	48, 477, 501, 634, 1216, 2926, 3061, 3335
AsuC2I	CCSGG	2 cut(s)	1209, 3914
AsuHPI	GGTGA	6 cut(s)	781, 1057, 2055, 2091, 2875, 3946
AsuII	TTCGAA	2 cut(s)	589, 3430
AvaI	CYCGRG	3 cut(s)	2770, 3306, 3787
AvaII	GGWCC	6 cut(s)	48, 501, 634, 1216, 3061, 3335
AxyI	CCTNAGG	1 cut(s)	1432
BaeGI	GKGCMC	2 cut(s)	3307, 3736
BaeI	ACNNNNGTAYC	2 cut(s)	1644, 1677
BamHI	GGATCC	1 cut(s)	3884
BanI	GGYRCC	4 cut(s)	1056, 2220, 2688, 3106
BanII	GRGCYC	3 cut(s)	185, 192, 316
BarI	GAAGNNNNNNTAC	2 cut(s)	938, 970
BauI	CACGAG	3 cut(s)	317, 413, 3530
Bbv12I	GWGCWC	4 cut(s)	185, 316, 1394, 3736
BbvCI	CCTCAGC	1 cut(s)	1487
BceAI	ACGGC	3 cut(s)	443, 1087, 3953
BcgI	CGANNNNNNTGC	8 cut(s)	906, 940, 1292, 1326, 1580, 1614, 3500, 3534
BciT130I	CCWGG	7 cut(s)	560, 638, 1580, 2560, 3104, 3479, 3572
BciVI	GTATCC	1 cut(s)	1325
BclI	TGATCA	3 cut(s)	1198, 2367, 2536
BcnI	CCSGG	2 cut(s)	1209, 3914
BcoDI	GTCTC	4 cut(s)	945, 1405, 2607, 2769
BcuI	ACTAGT	1 cut(s)	1261
BfmI	CTRYAG	2 cut(s)	861, 3201
BfoI	RGCGCY	4 cut(s)	911, 2224, 2692, 3110
BfuI	GTATCC	1 cut(s)	1325
BglI	GCCNNNNNGGC	1 cut(s)	464
BglII	AGATCT	1 cut(s)	3755
BmcAI	AGTACT	2 cut(s)	572, 2387
Bme1390I	CCNGG	9 cut(s)	560, 638, 1209, 1580, 2560, 3104, 3479, 3572, 3914
Bme18I	GGWCC	6 cut(s)	48, 501, 634, 1216, 3061, 3335
BmeRI	GACNNNNNGTC	2 cut(s)	1133, 1780
BmeT110I	CYCGRG	3 cut(s)	2770, 3306, 3787
BmgT120I	GGNCC	8 cut(s)	48, 477, 501, 634, 1216, 2926, 3061, 3335
BmiI	GGNNCC	8 cut(s)	1058, 1921, 2222, 2690, 3108, 3474, 3483, 3886
BmrFI	CCNGG	9 cut(s)	560, 638, 1209, 1580, 2560, 3104, 3479, 3572, 3914
BplI	GAGNNNNNCTC	2 cut(s)	2457, 2489
BpmI	CTGGAG	3 cut(s)	1707, 2879, 3672
Bpu10I	CCTNAGC	1 cut(s)	1487
Bpu14I	TTCGAA	2 cut(s)	589, 3430
BpuEI	CTTGAG	2 cut(s)	86, 563
BpuMI	CCSGG	2 cut(s)	1209, 3914
BsaBI	GATNNNNATC	1 cut(s)	530
BsaHI	GRCGYC	5 cut(s)	600, 642, 2221, 2689, 3107
BsaI	GGTCTC	2 cut(s)	1405, 2769
BsaJI	CCNNGG	6 cut(s)	160, 924, 2796, 3102, 3468, 3477
BsaWI	WCCGGW	5 cut(s)	348, 503, 1256, 1812, 1974
BsaXI	ACNNNNNCTCC	6 cut(s)	139, 169, 303, 333, 444, 474
Bse118I	RCCGGY	4 cut(s)	206, 1812, 2112, 2910
Bse21I	CCTNAGG	1 cut(s)	1432
Bse3DI	GCAATG	8 cut(s)	1356, 1728, 1842, 2690, 3363, 3462, 3599, 3846
Bse8I	GATNNNNATC	1 cut(s)	530
BseAI	TCCGGA	1 cut(s)	1974
BseBI	CCWGG	7 cut(s)	560, 638, 1580, 2560, 3104, 3479, 3572
BseDI	CCNNGG	6 cut(s)	160, 924, 2796, 3102, 3468, 3477
BseGI	GGATG	7 cut(s)	85, 501, 1312, 1971, 1983, 3350, 3922
BseJI	GATNNNNATC	1 cut(s)	530
BseMI	GCAATG	8 cut(s)	1356, 1728, 1842, 2690, 3363, 3462, 3599, 3846
BsePI	GCGCGC	1 cut(s)	3929
BseRI	GAGGAG	1 cut(s)	913
BseSI	GKGCMC	2 cut(s)	3307, 3736
BseYI	CCCAGC	4 cut(s)	106, 118, 1735, 2813
BsgI	GTGCAG	2 cut(s)	2403, 3105
Bsh1236I	CGCG	2 cut(s)	3929, 3931
BshFI	GGCC	8 cut(s)	63, 479, 548, 1567, 2035, 2801, 2822, 2928
BshNI	GGYRCC	4 cut(s)	1056, 2220, 2688, 3106
BshTI	ACCGGT	1 cut(s)	1812
BsiHKAI	GWGCWC	4 cut(s)	185, 316, 1394, 3736
BsiHKCI	CYCGRG	3 cut(s)	2770, 3306, 3787
BslFI	GGGAC	5 cut(s)	996, 1121, 1140, 3260, 3433
BsmAI	GTCTC	4 cut(s)	945, 1405, 2607, 2769
BsmFI	GGGAC	5 cut(s)	996, 1121, 1140, 3260, 3433
BsmI	GAATGC	3 cut(s)	391, 780, 2849
BsnI	GGCC	8 cut(s)	63, 479, 548, 1567, 2035, 2801, 2822, 2928
Bso31I	GGTCTC	2 cut(s)	1405, 2769
BsoBI	CYCGRG	3 cut(s)	2770, 3306, 3787
Bsp119I	TTCGAA	2 cut(s)	589, 3430
Bsp1286I	GDGCHC	6 cut(s)	185, 192, 316, 1394, 3307, 3736
Bsp13I	TCCGGA	1 cut(s)	1974
Bsp1407I	TGTACA	1 cut(s)	2632
BspANI	GGCC	8 cut(s)	63, 479, 548, 1567, 2035, 2801, 2822, 2928
BspEI	TCCGGA	1 cut(s)	1974
BspFNI	CGCG	2 cut(s)	3929, 3931
BspHI	TCATGA	1 cut(s)	2757
BspLI	GGNNCC	8 cut(s)	1058, 1921, 2222, 2690, 3108, 3474, 3483, 3886
BspPI	GGATC	8 cut(s)	83, 310, 539, 1418, 2284, 3182, 3879, 3892
BspT104I	TTCGAA	2 cut(s)	589, 3430
BspT107I	GGYRCC	4 cut(s)	1056, 2220, 2688, 3106
BspTNI	GGTCTC	2 cut(s)	1405, 2769
BsrBI	CCGCTC	2 cut(s)	511, 551
BsrDI	GCAATG	8 cut(s)	1356, 1728, 1842, 2690, 3363, 3462, 3599, 3846
BsrFI	RCCGGY	4 cut(s)	206, 1812, 2112, 2910
BsrGI	TGTACA	1 cut(s)	2632
BssAI	RCCGGY	4 cut(s)	206, 1812, 2112, 2910
BssECI	CCNNGG	6 cut(s)	160, 924, 2796, 3102, 3468, 3477
BssHII	GCGCGC	1 cut(s)	3929
BssNI	GRCGYC	5 cut(s)	600, 642, 2221, 2689, 3107
BssSI	CACGAG	3 cut(s)	317, 413, 3530
BssT1I	CCWWGG	3 cut(s)	160, 2796, 3468
Bst2BI	CACGAG	3 cut(s)	317, 413, 3530
Bst2UI	CCWGG	7 cut(s)	560, 638, 1580, 2560, 3104, 3479, 3572
Bst6I	CTCTTC	2 cut(s)	263, 4008
BstACI	GRCGYC	5 cut(s)	600, 642, 2221, 2689, 3107
BstAPI	GCANNNNNTGC	6 cut(s)	1766, 2792, 2972, 3083, 3731, 3740
BstAUI	TGTACA	1 cut(s)	2632
BstBI	TTCGAA	2 cut(s)	589, 3430
BstC8I	GCNNGC	9 cut(s)	2114, 2205, 2577, 2662, 3313, 3537, 3780, 3784, 3931
BstENI	CCTNNNNNAGG	2 cut(s)	1431, 4013
BstF5I	GGATG	7 cut(s)	85, 501, 1312, 1971, 1983, 3350, 3922
BstFNI	CGCG	2 cut(s)	3929, 3931
BstH2I	RGCGCY	4 cut(s)	911, 2224, 2692, 3110
BstHHI	GCGC	8 cut(s)	910, 1151, 2030, 2223, 2691, 3109, 3931, 3933
BstMAI	GTCTC	4 cut(s)	945, 1405, 2607, 2769
BstNI	CCWGG	7 cut(s)	560, 638, 1580, 2560, 3104, 3479, 3572
BstNSI	RCATGY	3 cut(s)	2795, 3245, 3845
BstSCI	CCNGG	9 cut(s)	558, 636, 1207, 1578, 2558, 3102, 3477, 3570, 3912
BstSFI	CTRYAG	2 cut(s)	861, 3201
BstSLI	GKGCMC	2 cut(s)	3307, 3736
BstUI	CGCG	2 cut(s)	3929, 3931
BstX2I	RGATCY	3 cut(s)	2276, 3755, 3884
BstXI	CCANNNNNNTGG	5 cut(s)	1363, 2813, 2936, 3805, 3971
BstYI	RGATCY	3 cut(s)	2276, 3755, 3884
Bsu36I	CCTNAGG	1 cut(s)	1432
BsuI	GTATCC	1 cut(s)	1325
BsuRI	GGCC	8 cut(s)	63, 479, 548, 1567, 2035, 2801, 2822, 2928
BtgZI	GCGATG	2 cut(s)	1069, 1835
BtsCI	GGATG	7 cut(s)	85, 501, 1312, 1971, 1983, 3350, 3922
BtsI	GCAGTG	4 cut(s)	44, 1381, 2664, 3858
Cac8I	GCNNGC	9 cut(s)	2114, 2205, 2577, 2662, 3313, 3537, 3780, 3784, 3931
CaiI	CAGNNNCTG	1 cut(s)	3542
CciI	TCATGA	1 cut(s)	2757
CfoI	GCGC	8 cut(s)	910, 1151, 2030, 2223, 2691, 3109, 3931, 3933
Cfr10I	RCCGGY	4 cut(s)	206, 1812, 2112, 2910
Cfr13I	GGNCC	8 cut(s)	48, 477, 501, 634, 1216, 2926, 3061, 3335
CseI	GACGC	1 cut(s)	650
CsiI	ACCWGGT	1 cut(s)	1578
Csp6I	GTAC	7 cut(s)	427, 436, 538, 571, 1889, 2386, 2633
CspAI	ACCGGT	1 cut(s)	1812
CviQI	GTAC	7 cut(s)	427, 436, 538, 571, 1889, 2386, 2633
DinI	GGCGCC	3 cut(s)	2222, 2690, 3108
DraI	TTTAAA	1 cut(s)	97
DraIII	CACNNNGTG	1 cut(s)	850
DriI	GACNNNNNGTC	2 cut(s)	1133, 1780
Eam1104I	CTCTTC	2 cut(s)	263, 4008
Eam1105I	GACNNNNNGTC	2 cut(s)	1133, 1780
EarI	CTCTTC	2 cut(s)	263, 4008
EciI	GGCGGA	1 cut(s)	182
Ecl136II	GAGCTC	2 cut(s)	183, 314
Eco130I	CCWWGG	3 cut(s)	160, 2796, 3468
Eco147I	AGGCCT	2 cut(s)	63, 2035
Eco24I	GRGCYC	3 cut(s)	185, 192, 316
Eco31I	GGTCTC	2 cut(s)	1405, 2769
Eco47I	GGWCC	6 cut(s)	48, 501, 634, 1216, 3061, 3335
Eco47III	AGCGCT	1 cut(s)	909
Eco53kI	GAGCTC	2 cut(s)	183, 314
Eco57I	CTGAAG	2 cut(s)	244, 3464
Eco81I	CCTNAGG	1 cut(s)	1432
Eco88I	CYCGRG	3 cut(s)	2770, 3306, 3787
EcoICRI	GAGCTC	2 cut(s)	183, 314
EcoNI	CCTNNNNNAGG	2 cut(s)	1431, 4013
EcoRII	CCWGG	7 cut(s)	558, 636, 1578, 2558, 3102, 3477, 3570
EcoT14I	CCWWGG	3 cut(s)	160, 2796, 3468
EcoT38I	GRGCYC	3 cut(s)	185, 192, 316
EgeI	GGCGCC	3 cut(s)	2222, 2690, 3108
EheI	GGCGCC	3 cut(s)	2222, 2690, 3108
ErhI	CCWWGG	3 cut(s)	160, 2796, 3468
FalI	AAGNNNNNCTT	6 cut(s)	813, 845, 1791, 1823, 1863, 1895
FaqI	GGGAC	5 cut(s)	996, 1121, 1140, 3260, 3433
FauI	CCCGC	3 cut(s)	244, 460, 2662
FauNDI	CATATG	2 cut(s)	2089, 2314
FbaI	TGATCA	3 cut(s)	1198, 2367, 2536
FblI	GTMKAC	1 cut(s)	3246
FokI	GGATG	7 cut(s)	92, 508, 1319, 1958, 1990, 3357, 3929
FriOI	GRGCYC	3 cut(s)	185, 192, 316
GlaI	GCGC	8 cut(s)	909, 1150, 2029, 2222, 2690, 3108, 3930, 3932
GsaI	CCCAGC	4 cut(s)	110, 122, 1739, 2817
GsuI	CTGGAG	3 cut(s)	1707, 2879, 3672
HaeII	RGCGCY	4 cut(s)	911, 2224, 2692, 3110
HaeIII	GGCC	8 cut(s)	63, 479, 548, 1567, 2035, 2801, 2822, 2928
HgaI	GACGC	1 cut(s)	650
HhaI	GCGC	8 cut(s)	910, 1151, 2030, 2223, 2691, 3109, 3931, 3933
Hin1I	GRCGYC	5 cut(s)	600, 642, 2221, 2689, 3107
Hin6I	GCGC	8 cut(s)	908, 1149, 2028, 2221, 2689, 3107, 3929, 3931
HinP1I	GCGC	8 cut(s)	908, 1149, 2028, 2221, 2689, 3107, 3929, 3931
HincII	GTYRAC	1 cut(s)	519
HindII	GTYRAC	1 cut(s)	519
HindIII	AAGCTT	1 cut(s)	947
HphI	GGTGA	6 cut(s)	781, 1057, 2055, 2091, 2875, 3946
Hpy166II	GTNNAC	5 cut(s)	501, 519, 2341, 3247, 3734
Hpy8I	GTNNAC	5 cut(s)	501, 519, 2341, 3247, 3734
Hpy99I	CGWCG	1 cut(s)	605
HpyCH4IV	ACGT	2 cut(s)	285, 600
HpySE526I	ACGT	2 cut(s)	285, 600
Hsp92I	GRCGYC	5 cut(s)	600, 642, 2221, 2689, 3107
HspAI	GCGC	8 cut(s)	908, 1149, 2028, 2221, 2689, 3107, 3929, 3931
KasI	GGCGCC	3 cut(s)	2220, 2688, 3106
Kpn2I	TCCGGA	1 cut(s)	1974
KroI	GCCGGC	1 cut(s)	2112
KroNI	GCCGGC	1 cut(s)	2114
Ksp22I	TGATCA	3 cut(s)	1198, 2367, 2536
LmnI	GCTCC	6 cut(s)	188, 311, 452, 989, 2200, 3481
MabI	ACCWGGT	1 cut(s)	1578
MaeII	ACGT	2 cut(s)	285, 600
MaeIII	GTNAC	9 cut(s)	3, 739, 953, 1252, 2390, 3205, 3287, 3611, 3923
MbiI	CCGCTC	2 cut(s)	511, 551
MboII	GAAGA	8 cut(s)	250, 346, 1019, 1192, 1604, 2419, 4025, 4031
MfeI	CAATTG	4 cut(s)	297, 1679, 3675, 3801
MflI	RGATCY	3 cut(s)	2276, 3755, 3884
MhlI	GDGCHC	6 cut(s)	185, 192, 316, 1394, 3307, 3736
Mly113I	GGCGCC	3 cut(s)	2221, 2689, 3107
MlyI	GAGTC	7 cut(s)	194, 978, 1256, 1519, 1769, 1790, 3313
MmeI	TCCRAC	9 cut(s)	258, 289, 430, 473, 1764, 1918, 2254, 3054, 3952
MroI	TCCGGA	1 cut(s)	1974
MroNI	GCCGGC	1 cut(s)	2112
MroXI	GAANNNNTTC	3 cut(s)	1184, 1906, 2753
MseI	TTAA	7 cut(s)	96, 369, 1478, 1542, 2379, 2399, 3098
MslI	CAYNNNNRTG	3 cut(s)	1976, 3803, 3851
MspA1I	CMGCKG	4 cut(s)	760, 1175, 1346, 2417
MspR9I	CCNGG	9 cut(s)	560, 638, 1209, 1580, 2560, 3104, 3479, 3572, 3914
MunI	CAATTG	4 cut(s)	297, 1679, 3675, 3801
Mva1269I	GAATGC	3 cut(s)	391, 780, 2849
MvaI	CCWGG	7 cut(s)	560, 638, 1580, 2560, 3104, 3479, 3572
MvnI	CGCG	2 cut(s)	3929, 3931
NaeI	GCCGGC	1 cut(s)	2114
NarI	GGCGCC	3 cut(s)	2221, 2689, 3107
NciI	CCSGG	2 cut(s)	1209, 3914
NdeI	CATATG	2 cut(s)	2089, 2314
NgoMIV	GCCGGC	1 cut(s)	2112
NlaIV	GGNNCC	8 cut(s)	1058, 1921, 2222, 2690, 3108, 3474, 3483, 3886
NmeAIII	GCCGAG	2 cut(s)	505, 905
NmuCI	GTSAC	4 cut(s)	3, 739, 3205, 3287
NspI	RCATGY	3 cut(s)	2795, 3245, 3845
NspV	TTCGAA	2 cut(s)	589, 3430
OliI	CACNNNNGTG	1 cut(s)	3803
PagI	TCATGA	1 cut(s)	2757
PauI	GCGCGC	1 cut(s)	3929
PceI	AGGCCT	2 cut(s)	63, 2035
PciI	ACATGT	1 cut(s)	3841
PcsI	WCGNNNNNNNCGW	2 cut(s)	315, 3136
PctI	GAATGC	3 cut(s)	391, 780, 2849
PdiI	GCCGGC	1 cut(s)	2114
PdmI	GAANNNNTTC	3 cut(s)	1184, 1906, 2753
PfoI	TCCNGGA	1 cut(s)	636
PinAI	ACCGGT	1 cut(s)	1812
PleI	GAGTC	7 cut(s)	194, 978, 1255, 1518, 1769, 1789, 3313
PluTI	GGCGCC	3 cut(s)	2224, 2692, 3110
PpsI	GAGTC	7 cut(s)	194, 978, 1255, 1518, 1769, 1789, 3313
PscI	ACATGT	1 cut(s)	3841
PshBI	ATTAAT	1 cut(s)	2379
Psp124BI	GAGCTC	2 cut(s)	185, 316
Psp6I	CCWGG	7 cut(s)	558, 636, 1578, 2558, 3102, 3477, 3570
PspFI	CCCAGC	4 cut(s)	106, 118, 1735, 2813
PspGI	CCWGG	7 cut(s)	558, 636, 1578, 2558, 3102, 3477, 3570
PspN4I	GGNNCC	8 cut(s)	1058, 1921, 2222, 2690, 3108, 3474, 3483, 3886
PspPI	GGNCC	8 cut(s)	48, 477, 501, 634, 1216, 2926, 3061, 3335
PstNI	CAGNNNCTG	1 cut(s)	3542
PsuI	RGATCY	3 cut(s)	2276, 3755, 3884
PteI	GCGCGC	1 cut(s)	3929
PvuII	CAGCTG	3 cut(s)	1175, 1346, 2417
RsaI	GTAC	7 cut(s)	428, 437, 539, 572, 1890, 2387, 2634
RsaNI	GTAC	7 cut(s)	427, 436, 538, 571, 1889, 2386, 2633
RseI	CAYNNNNRTG	3 cut(s)	1976, 3803, 3851
SacI	GAGCTC	2 cut(s)	185, 316
SaqAI	TTAA	7 cut(s)	96, 369, 1478, 1542, 2379, 2399, 3098
Sau96I	GGNCC	8 cut(s)	48, 477, 501, 634, 1216, 2926, 3061, 3335
ScaI	AGTACT	2 cut(s)	572, 2387
SchI	GAGTC	7 cut(s)	194, 978, 1256, 1519, 1769, 1790, 3313
ScrFI	CCNGG	9 cut(s)	560, 638, 1209, 1580, 2560, 3104, 3479, 3572, 3914
SduI	GDGCHC	6 cut(s)	185, 192, 316, 1394, 3307, 3736
SexAI	ACCWGGT	1 cut(s)	1578
SfcI	CTRYAG	2 cut(s)	861, 3201
SfoI	GGCGCC	3 cut(s)	2222, 2690, 3108
SfuI	TTCGAA	2 cut(s)	589, 3430
SgrAI	CRCCGGYG	2 cut(s)	206, 2910
SinI	GGWCC	6 cut(s)	48, 501, 634, 1216, 3061, 3335
SmiMI	CAYNNNNRTG	3 cut(s)	1976, 3803, 3851
SmlI	CTYRAG	2 cut(s)	65, 542
SmoI	CTYRAG	2 cut(s)	65, 542
SpeI	ACTAGT	1 cut(s)	1261
SseBI	AGGCCT	2 cut(s)	63, 2035
SspDI	GGCGCC	3 cut(s)	2220, 2688, 3106
SspI	AATATT	1 cut(s)	3517
SstI	GAGCTC	2 cut(s)	185, 316
StuI	AGGCCT	2 cut(s)	63, 2035
StyD4I	CCNGG	9 cut(s)	558, 636, 1207, 1578, 2558, 3102, 3477, 3570, 3912
StyI	CCWWGG	3 cut(s)	160, 2796, 3468
TaiI	ACGT	2 cut(s)	288, 603
TatI	WGTACW	5 cut(s)	435, 570, 1888, 2385, 2632
TauI	GCSGC	5 cut(s)	213, 216, 514, 551, 1567
Tru1I	TTAA	7 cut(s)	96, 369, 1478, 1542, 2379, 2399, 3098
Tru9I	TTAA	7 cut(s)	96, 369, 1478, 1542, 2379, 2399, 3098
TseFI	GTSAC	4 cut(s)	3, 739, 3205, 3287
Tsp45I	GTSAC	4 cut(s)	3, 739, 3205, 3287
TspGWI	ACGGA	2 cut(s)	596, 3275
VneI	GTGCAC	1 cut(s)	3732
VpaK11BI	GGWCC	6 cut(s)	48, 501, 634, 1216, 3061, 3335
VspI	ATTAAT	1 cut(s)	2379
XagI	CCTNNNNNAGG	2 cut(s)	1431, 4013
XapI	RAATTY	2 cut(s)	870, 2080
XceI	RCATGY	3 cut(s)	2795, 3245, 3845
XmiI	GTMKAC	1 cut(s)	3246
XmnI	GAANNNNTTC	3 cut(s)	1184, 1906, 2753
ZraI	GACGTC	1 cut(s)	601
ZrmI	AGTACT	2 cut(s)	572, 2387

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.