Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0013415

Physical Location & Seq

Genomic Coordinates Forward (+)

34014060 .. 34019823

Gene Structure 5764 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000024279

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4173 bp

ATGTCACAAGATTCTCAGGGGATAAAGACGATTGAGCAGTGGAGATGGTCAGATATGCAAGGACTTGAGCTTGTGACTGACCCTCCTCCTCCTTCTTCTTCTTCTTCTTCTGACCCATCTAAAACCAACCCAACAACAACAACAACAACTGCTAAAGAGGCAAGAGAAGCTCAAGCAATGGAGAGCTCTGCTGAGCCAACAACAAAGAAGGAGTCTAATGACAGCGGTGGAGGTGAGAAGCCTGAGGCTGTTCCAAGCATAGGCTTCGGACAGGTTTTCAGATTCGCTGATGGATTGGATTACGTTTTGATGGGAATTGGGTCTGTCGGCGCAATCGTGCATGGTTGTTCCTTGCCTATCTTTCTTCGCTTCTTTGCTGATCTTGTCAACTCTTTCGGGGCAAATGCGAATGACATGGACAAGATGATGCAAGAGGTTTTGAAGTATGCATTGTACTTTCTTGTTGTGGGAGCTGCAATATGGGCTTCTTCATGGGCAGAGATATCGTGTTGGATGTGGACCGGAGAAAGGCAATCGACCAAGATGAGGATCAAGTACCTTGAAGCTGCTTTGAGCCAGGACATTCAGTTTTTCGATACAGAGGTTCGAACCTCCGATGTCGTTTTTGCCATCAACACTGATGCAGTGATGGTCCAAGATGCTATTAGCGAGAAGTTGGGTAATTTCATTCACTACATGGCGACATTTGTATCTGGATTTGTGGTGGGTTTCACTGCTGTGTGGCAATTAGCTCTTGTGACTCTAGCAGTGGTGCCCCTGATAGCTGTGATTGGAGCCATTCACACCACCACATTAGCCAAACTCTCAGGGAAGAGCCAAGAGGCTCTTTCACAAGCAGGACACACTGTAGAACAGACTGTAGTTCAAATTCGAGTTGTCATGTCGTACGTTGGGGAATCAAGAGCACTAGAAGCATATTCCTCAGCATTGAGAATTGCCCAGAAGCTTGGTTACAAGACCGGATTTGCTAAAGGAATGGGACTTGGAGCAACTTACTTTGTAGTCTTCTGCTGTTATGCACTTCTTTTATGGTATGGGGGTTATCTTGTTAGGCACCACTTCACCAATGGAGGTCTTGCCATAGCCACCATGTTTTCGGTCATGATAGGTGGACTGGCTTTGGGACAATCTGCTCCAAGCATGGGCGCATTTGCAAAGGCTAAAGTTGCAGCTGCCAAGATATTTCGGATCATTGACCACAAGCCAGGCATGGACCGCAACAGTGAATCAGGGGTTGAATTGGAATCTGTTACAGGACTAGTGGAGCTGAAAAATGTGGATTTCTCCTACCCATCAAGGCAGGATGTTCGTATTCTCAACAATTTCTCTTTGAATGTTCCGGCTGGAAAAACGATAGCTTTGGTCGGTAGCAGTGGCTCTGGTAAAAGCACTGTGGTCTCCCTCATTGAGAGATTTTATGACCCCTCCTCAGGTAACTTAGCTCATTGTAATTGTTCTAGTAAGAGTTTTAACTTGCTCATGAGTTCATATATTTCTGGAGGTTTCTTCATTGTCTTAATATATTTCTTTATTGAACAAACCATTTCAGGACAAGTTCTGCTAGATGGACATGACATAAAGACATTGAAATTAAAGTGGCTAAGGCAGCAGATAGGGCTTGTGAGCCAAGAGCCTGCTCTGTTTGCTACCACCATTAAAGAGAATATACTTTTGGGGAGGCCTGATGCAGACCAAGTTGAGATTGAAGAAGCTGCGAGAGTTGCCAATGCTCATTCATTCATTGTCAAACTTCCTGATGGTTTTGATACTCAGGTAGGGGAAAGAGGAGTGCAACTGTCTGGAGGACAGAAGCAGAGGATAGCCATAGCAAGGGCAATGCTTAAAAACCCAGCAATCCTTCTCTTAGATGAGGCAACAAGTGCATTGGACTCTGAGTCGGAAAAGCTGGTGCAAGAAGCCCTGGACCGGTTTATGATAGGCAGAACAACTCTTGTCATTGCTCACCGCCTCTCAACCATTCGCAAAGCTGACCTTGTAGCTGTACTCCAGCAGGGAAGTGTTTCTGAAATTGGAACCCATGATGAACTCTTTTCTAAAGGAGAAAATGGTGTTTATGCCAAGCTCATTCGAATGCAAGAGGCAGCTCATGAGACTGCTCTCAACAATGCCAGAAAGAGTAGTGCTAGGCCTTCTAGTGCAAGAAACTCTGTTAGCTCACCAATAATCGCCCGAAATTCTTCATACGGTCGATCGCCATATTCACGCAGGCTCTCTGACTTTTCCACTTCTGATTTTAGTCTCTCCCTTGATGCCACACACCCCAATTACCGTCTAGAAAAGCTTGCCTTCAAGGAGCAAGCCAGTTCCTTCTGGCGCCTTGCCAAGATGAACTCTCCTGAATGGGTGTATGCATTAGTTGGTTCAATTGGCTCTGTTGTTTGTGGTTCTCTGAGTGCTTTCTTTGCATATGTTCTAAGTGCAGTCCTCAGTGTGTACTACAATCCGGACCATGATTTCATGATCAAGCAAATTAATAAGTACTGCTATTTGTTAATTGGACTTTCATCAGCTGCACTACTCTTCAACACGCTACAACATTCTTTCTGGGATATTGTGGGAGAAAATCTTACAAAACGGGTGAGGGAGAAAATGCTGACAGCAGTGTTGAAAAATGAAATGGCATGGTTTGATCAGGAGGAAAATGAGAGTGGCAGAATTGCTGCAAGGCTGGCCCTTGATGCCAACAATGTCAGATCAGCCATTGGAGACAGGATTTCTGTTATTGTACAGAACACAGCACTCATGCTAGTTGCTTGCACTGCAGGATTTGTTTTGCAATGGCGCCTTGCCCTTGTCCTCGTAGCTGTCTTCCCTGTTGTTGTTGCCGCCACAGTTTTGCAGAAAATGTTCATGACCGGTTTCTCGGGAGACCTGGAAGCTGCACATGCCAAGGCCACCCAACTAGCTGGGGAGGCCATAGCCAATGTAAGGACTGTTGCTGCATTCAACTCCGAGGGAAAAATTGTTGGTCTCTTTTCCTCCAACCTGCAGATTCCCCTGCGTCGGTGTTTTTGGAAGGGTCAGATTGCTGGAAGTGGATTTGGGATAGCTCAGTTTGCACTTTATGCCTCCTATGCTCTAGGTCTTTGGTATGCTTCCTGGCTTGTAAAGCATGGAATCTCTGATTTCTCAAAGGCGATCCGAGTTTTCATGGTCCTCATGGTATCTGCTAACGGTGCAGCAGAGACACTAACCCTGGCTCCTGACTTTATTAAGGGTGGTCGAGCCATGCAATCAGTTTTTGAGCTCCTTGACCGCAAAACTGAAATTGAACCTGATGATATTGATGCTACTGCAGTGCCTGACCGTCTGCGTGGAGAAGTTGAGTTTAAGCATGTAGACTTTTCGTATCCTTCTCGACCTGATGTTCCTGTTTTTCGTGATCTCTCCCTCCGTGCCCGAGCAGGCAAGACTCTTGCTCTTGTTGGTCCTAGTGGGTGTGGAAAGAGCTCAGTCATTTCATTAGTACAGCGGTTTTATGACCCAACATCAGGAAGAGTTATTATTGATGGAAAAGATATTCGCAAATACAATCTCAAGTCTCTCAGAAGGCACATTGCTGTGGTTCCCCAGGAGCCGTGCCTCTTTGCTACAACTATATATGAAAATATCGCTTATGGGCACGAGTCAGCAACTGAGGCTGAGATCATTGAAGCTGCTAATCTAGCCAATGCTCACAAGTTTGTATCTGCATTGCCAGATGGCTACAAAACGTTTGTTGGTGAGAGGGGAATTCAACTATCTGGAGGACAGAAACAAAGGATTGCTATTGCCAGGGCTTTACTGAGGAAGGCAGAGCTTATGCTTCTCGATGAAGCCACAAGTGCACTTGACGCCGAGTCCGAGAGATCTATTCAAGAAGCTCTGGAGCGCGCTTGTTCAGGAAAAACCACAATTGTGGTTGCACACAGACTATCAACTATTAGAAATGCCAATGTTATTGCAGTAATTGATGATGGGAAAGTGGCAGAGCAAGGATCACACAACCATCTATTGAAAAACTACCCGGACGGGTGTTACGCCCGCATGATACAGCTACAAAGATTTTCACACAGCCAGGCCATTGGAATAGCTTCAGGTTCAAGTTCTTCAGTAAGACCAAGGGAAGAGGAAGAAAGAGAAGGCAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006810 GO:0007275 GO:0008150 GO:0008361 GO:0009314 GO:0009411 GO:0009416 GO:0009506 GO:0009605 GO:0009606 GO:0009607 GO:0009624 GO:0009628 GO:0009629 GO:0009630 GO:0009637 GO:0009639 GO:0009640 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009908 GO:0009914 GO:0009926 GO:0009958 GO:0009966 GO:0009987 GO:0010033 GO:0010315 GO:0010328 GO:0010329 GO:0010646 GO:0010817 GO:0010928 GO:0015399 GO:0015405 GO:0015562 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022414 GO:0022804 GO:0022857 GO:0023051 GO:0030054 GO:0032501 GO:0032502 GO:0032535 GO:0042221 GO:0042623 GO:0042626 GO:0043207 GO:0043473 GO:0043476 GO:0043478 GO:0043479 GO:0043480 GO:0043481 GO:0043492 GO:0044464 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048583 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055044 GO:0055085 GO:0060918 GO:0060919 GO:0061458 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0080161 GO:0090066 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1391

Amino Acids

151.56

Weight (kDa)

7.82

Isoelectric Point (pI)

37.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	106 - 377	1.3e-52	ABC transporter transmembrane region
ABC_tran	PF00005	446 - 487	5.1e-09	ABC transporter
ABC_tran	PF00005	519 - 634	1.7e-19	ABC transporter
ABC_membrane	PF00664	803 - 1069	6.1e-53	ABC transporter transmembrane region
ABC_tran	PF00005	1141 - 1289	2.6e-34	ABC transporter
SMC_N	PF02463	1177 - 1329	5.5e-06	RecF/RecN/SMC N terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0013415)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36910
fragaria_vesca	FvH4_6g16450 FvH4_6g16450 FvH4_6g16450
malus_domestica	MD04G1092800.v1.1
prunus_persica	Prupe.7G129600_v2.0.a1
pyrus_communis	pycom04g08730 pycom04g08740 pycom12g11150
rosa_chinensis	RchiOBHm_Chr3g0470311
rosa_laevigata	RLG00000024279
rosa_multiflora	Rmu_sc0001093.1_g000001
rosa_roxburghii	Rroxscaffold_6G00410950
rosa_rugosa	Rorug03G0108000
rosa_samantha	Rh3AG157800 Rh3BG182100 Rh3DG192800
rosa_wichuraiana	Rw3G015010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	3036
AccB1I	GGYRCC	4 cut(s)	772, 1074, 2355, 2823
AccI	GTMKAC	1 cut(s)	3381
AccII	CGCG	1 cut(s)	3915
AccIII	TCCGGA	1 cut(s)	2485
AciI	CCGC	7 cut(s)	225, 1237, 1987, 2868, 3298, 3514, 4066
AclI	AACGTT	1 cut(s)	3755
AclWI	GGATC	4 cut(s)	557, 1217, 3175, 4027
AcsI	RAATTY	3 cut(s)	888, 2215, 3774
AcuI	CTGAAG	2 cut(s)	4101, 4116
AcyI	GRCGYC	3 cut(s)	2356, 2824, 3876
AfaI	GTAC	8 cut(s)	455, 557, 908, 2025, 2477, 2522, 2769, 3510
AgeI	ACCGGT	2 cut(s)	1947, 2897
AhdI	GACNNNNNGTC	2 cut(s)	1915, 3880
AhlI	ACTAGT	1 cut(s)	1279
AjnI	CCWGG	9 cut(s)	576, 1225, 1941, 2913, 3140, 3237, 3612, 3815, 4098
AjuI	GAANNNNNNNTTGG	2 cut(s)	1676, 1708
AleI	CACNNNNGTG	2 cut(s)	737, 3938
Alw21I	GWGCWC	5 cut(s)	188, 928, 3291, 3494, 3871
Alw26I	GTCTC	8 cut(s)	1423, 2126, 2285, 2742, 2904, 3017, 3221, 3588
Alw44I	GTGCAC	1 cut(s)	3867
AlwI	GGATC	4 cut(s)	557, 1217, 3175, 4027
AlwNI	CAGNNNCTG	2 cut(s)	3344, 3677
Ama87I	CYCGRG	2 cut(s)	2905, 3441
Aor13HI	TCCGGA	1 cut(s)	2485
AoxI	GGCC	6 cut(s)	1700, 2168, 2712, 2934, 2955, 4101
ApaLI	GTGCAC	1 cut(s)	3867
ApoI	RAATTY	3 cut(s)	888, 2215, 3774
ArsI	GACNNNNNNTTYG	2 cut(s)	197, 229
AseI	ATTAAT	1 cut(s)	2514
AsiGI	ACCGGT	2 cut(s)	1947, 2897
Asp700I	GAANNNNTTC	4 cut(s)	2041, 2218, 2888, 4114
AspLEI	GCGC	6 cut(s)	332, 1169, 2358, 2826, 3915, 3917
AspS9I	GGNCC	8 cut(s)	519, 652, 1234, 1945, 2488, 2713, 3196, 3470
AsuC2I	CCSGG	1 cut(s)	4049
AsuHPI	GGTGA	6 cut(s)	245, 1075, 1976, 2190, 2632, 3776
AsuII	TTCGAA	2 cut(s)	607, 2110
AvaI	CYCGRG	2 cut(s)	2905, 3441
AvaII	GGWCC	7 cut(s)	519, 652, 1234, 1945, 2488, 3196, 3470
AxyI	CCTNAGG	2 cut(s)	243, 1450
BaeGI	GKGCMC	4 cut(s)	777, 3442, 3666, 3871
BaeI	ACNNNNGTAYC	6 cut(s)	588, 621, 693, 726, 1779, 1812
BanI	GGYRCC	4 cut(s)	772, 1074, 2355, 2823
BanII	GRGCYC	3 cut(s)	188, 3291, 3494
BarI	GAAGNNNNNNTAC	2 cut(s)	956, 988
BauI	CACGAG	1 cut(s)	3665
BbsI	GAAGAC	2 cut(s)	1018, 2842
Bbv12I	GWGCWC	5 cut(s)	188, 928, 3291, 3494, 3871
BbvCI	CCTCAGC	1 cut(s)	943
BceAI	ACGGC	1 cut(s)	3604
BcgI	CGANNNNNNTGC	6 cut(s)	247, 281, 486, 520, 1310, 1344
BciT130I	CCWGG	9 cut(s)	578, 1227, 1943, 2915, 3142, 3239, 3614, 3817, 4100
BciVI	GTATCC	1 cut(s)	3402
BclI	TGATCA	2 cut(s)	2502, 2671
BcnI	CCSGG	1 cut(s)	4049
BcoDI	GTCTC	8 cut(s)	1423, 2126, 2285, 2742, 2904, 3017, 3221, 3588
BcuI	ACTAGT	1 cut(s)	1279
BfmI	CTRYAG	5 cut(s)	867, 879, 2802, 3029, 3336
BfoI	RGCGCY	2 cut(s)	2359, 2827
BfuAI	ACCTGC	1 cut(s)	3036
BfuI	GTATCC	1 cut(s)	3402
BglII	AGATCT	1 cut(s)	3890
BlpI	GCTNAGC	1 cut(s)	192
BmcAI	AGTACT	1 cut(s)	2522
Bme18I	GGWCC	7 cut(s)	519, 652, 1234, 1945, 2488, 3196, 3470
BmeRI	GACNNNNNGTC	2 cut(s)	1915, 3880
BmeT110I	CYCGRG	2 cut(s)	2905, 3441
BmgT120I	GGNCC	8 cut(s)	519, 652, 1234, 1945, 2488, 2713, 3196, 3470
BmiI	GGNNCC	9 cut(s)	774, 796, 1076, 2056, 2357, 2825, 3243, 3609, 3618
BmsI	GCATC	7 cut(s)	417, 631, 649, 1696, 2281, 2710, 3319
BpiI	GAAGAC	2 cut(s)	1018, 2842
BpmI	CTGGAG	5 cut(s)	1539, 1842, 2012, 3807, 3929
Bpu10I	CCTNAGC	2 cut(s)	943, 1622
Bpu1102I	GCTNAGC	1 cut(s)	192
Bpu14I	TTCGAA	2 cut(s)	607, 2110
BpuEI	CTTGAG	3 cut(s)	86, 156, 3563
BpuMI	CCSGG	1 cut(s)	4049
BsaBI	GATNNNNATC	1 cut(s)	548
BsaHI	GRCGYC	3 cut(s)	2356, 2824, 3876
BsaI	GGTCTC	3 cut(s)	1423, 2904, 3017
BsaJI	CCNNGG	7 cut(s)	1941, 2931, 2994, 3237, 3612, 3816, 4142
BsaWI	WCCGGW	5 cut(s)	521, 980, 1947, 2485, 2897
BsaXI	ACNNNNNCTCC	4 cut(s)	67, 97, 3226, 3256
Bse118I	RCCGGY	2 cut(s)	1947, 2897
Bse1I	ACTGG	2 cut(s)	1140, 2343
Bse21I	CCTNAGG	2 cut(s)	243, 1450
Bse3DI	GCAATG	6 cut(s)	183, 1863, 1977, 2825, 3597, 3734
Bse8I	GATNNNNATC	1 cut(s)	548
BseAI	TCCGGA	1 cut(s)	2485
BseBI	CCWGG	9 cut(s)	578, 1227, 1943, 2915, 3142, 3239, 3614, 3817, 4100
BseDI	CCNNGG	7 cut(s)	1941, 2931, 2994, 3237, 3612, 3816, 4142
BseGI	GGATG	2 cut(s)	519, 1330
BseJI	GATNNNNATC	1 cut(s)	548
BseMI	GCAATG	6 cut(s)	183, 1863, 1977, 2825, 3597, 3734
BseNI	ACTGG	2 cut(s)	1140, 2343
BsePI	GCGCGC	1 cut(s)	3913
BseRI	GAGGAG	4 cut(s)	75, 78, 1438, 1821
BseSI	GKGCMC	4 cut(s)	777, 3442, 3666, 3871
BseYI	CCCAGC	2 cut(s)	1870, 2948
BsgI	GTGCAG	4 cut(s)	2481, 2538, 2907, 3240
Bsh1236I	CGCG	1 cut(s)	3915
Bsh1285I	CGRYCG	2 cut(s)	2230, 2234
BshFI	GGCC	6 cut(s)	1702, 2170, 2714, 2936, 2957, 4103
BshNI	GGYRCC	4 cut(s)	772, 1074, 2355, 2823
BshTI	ACCGGT	2 cut(s)	1947, 2897
BsiEI	CGRYCG	2 cut(s)	2230, 2234
BsiHKAI	GWGCWC	5 cut(s)	188, 928, 3291, 3494, 3871
BsiHKCI	CYCGRG	2 cut(s)	2905, 3441
BsiSI	CCGG	7 cut(s)	522, 981, 1361, 1948, 2486, 2898, 4049
BsiWI	CGTACG	1 cut(s)	906
BslFI	GGGAC	2 cut(s)	1014, 1158
BsmAI	GTCTC	8 cut(s)	1423, 2126, 2285, 2742, 2904, 3017, 3221, 3588
BsmFI	GGGAC	2 cut(s)	1014, 1158
BsmI	GAATGC	2 cut(s)	2118, 2984
BsnI	GGCC	6 cut(s)	1702, 2170, 2714, 2936, 2957, 4103
Bso31I	GGTCTC	3 cut(s)	1423, 2904, 3017
BsoBI	CYCGRG	2 cut(s)	2905, 3441
Bsp119I	TTCGAA	2 cut(s)	607, 2110
Bsp1286I	GDGCHC	8 cut(s)	188, 777, 928, 3291, 3442, 3494, 3666, 3871
Bsp13I	TCCGGA	1 cut(s)	2485
Bsp1407I	TGTACA	1 cut(s)	2767
Bsp1720I	GCTNAGC	1 cut(s)	192
BspACI	CCGC	7 cut(s)	225, 1237, 1987, 2868, 3298, 3514, 4066
BspANI	GGCC	6 cut(s)	1702, 2170, 2714, 2936, 2957, 4103
BspEI	TCCGGA	1 cut(s)	2485
BspFNI	CGCG	1 cut(s)	3915
BspHI	TCATGA	5 cut(s)	1122, 1500, 2128, 2499, 2892
BspLI	GGNNCC	9 cut(s)	774, 796, 1076, 2056, 2357, 2825, 3243, 3609, 3618
BspMAI	CTGCAG	3 cut(s)	2806, 3033, 3340
BspMI	ACCTGC	1 cut(s)	3036
BspPI	GGATC	4 cut(s)	557, 1217, 3175, 4027
BspQI	GCTCTTC	1 cut(s)	827
BspT104I	TTCGAA	2 cut(s)	607, 2110
BspT107I	GGYRCC	4 cut(s)	772, 1074, 2355, 2823
BspTNI	GGTCTC	3 cut(s)	1423, 2904, 3017
BsrDI	GCAATG	6 cut(s)	183, 1863, 1977, 2825, 3597, 3734
BsrFI	RCCGGY	2 cut(s)	1947, 2897
BsrGI	TGTACA	1 cut(s)	2767
BsrI	ACTGG	2 cut(s)	1140, 2343
BssAI	RCCGGY	2 cut(s)	1947, 2897
BssECI	CCNNGG	7 cut(s)	1941, 2931, 2994, 3237, 3612, 3816, 4142
BssHII	GCGCGC	1 cut(s)	3913
BssNI	GRCGYC	3 cut(s)	2356, 2824, 3876
BssSI	CACGAG	1 cut(s)	3665
BssT1I	CCWWGG	2 cut(s)	2931, 4142
Bst2BI	CACGAG	1 cut(s)	3665
Bst2UI	CCWGG	9 cut(s)	578, 1227, 1943, 2915, 3142, 3239, 3614, 3817, 4100
Bst6I	CTCTTC	4 cut(s)	827, 2567, 3532, 4143
BstACI	GRCGYC	3 cut(s)	2356, 2824, 3876
BstAPI	GCANNNNNTGC	2 cut(s)	1901, 3107
BstAUI	TGTACA	1 cut(s)	2767
BstBI	TTCGAA	2 cut(s)	607, 2110
BstC8I	GCNNGC	9 cut(s)	1656, 2249, 2325, 2340, 2712, 2797, 3448, 3915, 4066
BstF5I	GGATG	2 cut(s)	519, 1330
BstFNI	CGCG	1 cut(s)	3915
BstH2I	RGCGCY	2 cut(s)	2359, 2827
BstHHI	GCGC	6 cut(s)	332, 1169, 2358, 2826, 3915, 3917
BstMAI	GTCTC	8 cut(s)	1423, 2126, 2285, 2742, 2904, 3017, 3221, 3588
BstMCI	CGRYCG	2 cut(s)	2230, 2234
BstNI	CCWGG	9 cut(s)	578, 1227, 1943, 2915, 3142, 3239, 3614, 3817, 4100
BstNSI	RCATGY	2 cut(s)	2930, 3380
BstSFI	CTRYAG	5 cut(s)	867, 879, 2802, 3029, 3336
BstSLI	GKGCMC	4 cut(s)	777, 3442, 3666, 3871
BstUI	CGCG	1 cut(s)	3915
BstV2I	GAAGAC	2 cut(s)	1018, 2842
BstX2I	RGATCY	1 cut(s)	3890
BstXI	CCANNNNNNTGG	4 cut(s)	968, 2948, 3940, 4106
BstYI	RGATCY	1 cut(s)	3890
Bsu36I	CCTNAGG	2 cut(s)	243, 1450
BsuI	GTATCC	1 cut(s)	3402
BsuRI	GGCC	6 cut(s)	1702, 2170, 2714, 2936, 2957, 4103
BtsCI	GGATG	2 cut(s)	519, 1330
BtsI	GCAGTG	8 cut(s)	44, 651, 732, 774, 1399, 2649, 2799, 3345
BveI	ACCTGC	1 cut(s)	3036
Cac8I	GCNNGC	9 cut(s)	1656, 2249, 2325, 2340, 2712, 2797, 3448, 3915, 4066
CaiI	CAGNNNCTG	2 cut(s)	3344, 3677
CciI	TCATGA	5 cut(s)	1122, 1500, 2128, 2499, 2892
CfoI	GCGC	6 cut(s)	332, 1169, 2358, 2826, 3915, 3917
Cfr10I	RCCGGY	2 cut(s)	1947, 2897
Cfr13I	GGNCC	8 cut(s)	519, 652, 1234, 1945, 2488, 2713, 3196, 3470
CseI	GACGC	2 cut(s)	3032, 3884
Csp6I	GTAC	8 cut(s)	454, 556, 907, 2024, 2476, 2521, 2768, 3509
CspAI	ACCGGT	2 cut(s)	1947, 2897
CviQI	GTAC	8 cut(s)	454, 556, 907, 2024, 2476, 2521, 2768, 3509
DinI	GGCGCC	2 cut(s)	2357, 2825
DriI	GACNNNNNGTC	2 cut(s)	1915, 3880
Eam1104I	CTCTTC	4 cut(s)	827, 2567, 3532, 4143
Eam1105I	GACNNNNNGTC	2 cut(s)	1915, 3880
EarI	CTCTTC	4 cut(s)	827, 2567, 3532, 4143
Ecl136II	GAGCTC	3 cut(s)	186, 3289, 3492
Eco130I	CCWWGG	2 cut(s)	2931, 4142
Eco147I	AGGCCT	2 cut(s)	1702, 2170
Eco24I	GRGCYC	3 cut(s)	188, 3291, 3494
Eco31I	GGTCTC	3 cut(s)	1423, 2904, 3017
Eco32I	GATATC	1 cut(s)	504
Eco47I	GGWCC	7 cut(s)	519, 652, 1234, 1945, 2488, 3196, 3470
Eco53kI	GAGCTC	3 cut(s)	186, 3289, 3492
Eco57I	CTGAAG	2 cut(s)	4101, 4116
Eco81I	CCTNAGG	2 cut(s)	243, 1450
Eco88I	CYCGRG	2 cut(s)	2905, 3441
EcoICRI	GAGCTC	3 cut(s)	186, 3289, 3492
EcoRI	GAATTC	1 cut(s)	3774
EcoRII	CCWGG	9 cut(s)	576, 1225, 1941, 2913, 3140, 3237, 3612, 3815, 4098
EcoRV	GATATC	1 cut(s)	504
EcoT14I	CCWWGG	2 cut(s)	2931, 4142
EcoT22I	ATGCAT	2 cut(s)	451, 2395
EcoT38I	GRGCYC	3 cut(s)	188, 3291, 3494
EgeI	GGCGCC	2 cut(s)	2357, 2825
EheI	GGCGCC	2 cut(s)	2357, 2825
ErhI	CCWWGG	2 cut(s)	2931, 4142
FalI	AAGNNNNNCTT	6 cut(s)	831, 863, 1998, 2030, 2312, 2344
FaqI	GGGAC	2 cut(s)	1014, 1158
FauI	CCCGC	1 cut(s)	4073
FauNDI	CATATG	1 cut(s)	2449
FbaI	TGATCA	2 cut(s)	2502, 2671
FblI	GTMKAC	1 cut(s)	3381
FokI	GGATG	2 cut(s)	526, 1337
FriOI	GRGCYC	3 cut(s)	188, 3291, 3494
GlaI	GCGC	6 cut(s)	331, 1168, 2357, 2825, 3914, 3916
GsaI	CCCAGC	2 cut(s)	1874, 2952
GsuI	CTGGAG	5 cut(s)	1539, 1842, 2012, 3807, 3929
HaeII	RGCGCY	2 cut(s)	2359, 2827
HaeIII	GGCC	6 cut(s)	1702, 2170, 2714, 2936, 2957, 4103
HapII	CCGG	7 cut(s)	522, 981, 1361, 1948, 2486, 2898, 4049
HgaI	GACGC	2 cut(s)	3032, 3884
HhaI	GCGC	6 cut(s)	332, 1169, 2358, 2826, 3915, 3917
Hin1I	GRCGYC	3 cut(s)	2356, 2824, 3876
Hin6I	GCGC	6 cut(s)	330, 1167, 2356, 2824, 3913, 3915
HinP1I	GCGC	6 cut(s)	330, 1167, 2356, 2824, 3913, 3915
HincII	GTYRAC	1 cut(s)	388
HindII	GTYRAC	1 cut(s)	388
HindIII	AAGCTT	2 cut(s)	965, 2321
HpaII	CCGG	7 cut(s)	522, 981, 1361, 1948, 2486, 2898, 4049
HphI	GGTGA	6 cut(s)	245, 1075, 1976, 2190, 2632, 3776
Hpy166II	GTNNAC	6 cut(s)	388, 519, 1133, 2476, 3382, 3869
Hpy8I	GTNNAC	6 cut(s)	388, 519, 1133, 2476, 3382, 3869
Hpy99I	CGWCG	1 cut(s)	3048
HpyCH4IV	ACGT	3 cut(s)	303, 909, 3755
HpySE526I	ACGT	3 cut(s)	303, 909, 3755
Hsp92I	GRCGYC	3 cut(s)	2356, 2824, 3876
HspAI	GCGC	6 cut(s)	330, 1167, 2356, 2824, 3913, 3915
KasI	GGCGCC	2 cut(s)	2355, 2823
Kpn2I	TCCGGA	1 cut(s)	2485
Ksp22I	TGATCA	2 cut(s)	2502, 2671
LguI	GCTCTTC	1 cut(s)	827
LweI	GCATC	7 cut(s)	417, 631, 649, 1696, 2281, 2710, 3319
MaeII	ACGT	3 cut(s)	303, 909, 3755
MaeIII	GTNAC	7 cut(s)	3, 73, 757, 971, 1270, 1454, 4058
MfeI	CAATTG	2 cut(s)	2406, 3936
MflI	RGATCY	1 cut(s)	3890
MhlI	GDGCHC	8 cut(s)	188, 777, 928, 3291, 3442, 3494, 3666, 3871
Mly113I	GGCGCC	2 cut(s)	2356, 2824
MlyI	GAGTC	7 cut(s)	221, 754, 1904, 1925, 3448, 3677, 3890
MmeI	TCCRAC	3 cut(s)	491, 1899, 3048
Mph1103I	ATGCAT	2 cut(s)	451, 2395
MroI	TCCGGA	1 cut(s)	2485
MroXI	GAANNNNTTC	4 cut(s)	2041, 2218, 2888, 4114
MseI	TTAA	9 cut(s)	1491, 1538, 1613, 1677, 1863, 2514, 2534, 3255, 3372
MslI	CAYNNNNRTG	4 cut(s)	737, 2111, 3602, 3938
MspA1I	CMGCKG	4 cut(s)	225, 1193, 2552, 3514
MspI	CCGG	7 cut(s)	522, 981, 1361, 1948, 2486, 2898, 4049
MunI	CAATTG	2 cut(s)	2406, 3936
Mva1269I	GAATGC	2 cut(s)	2118, 2984
MvaI	CCWGG	9 cut(s)	578, 1227, 1943, 2915, 3142, 3239, 3614, 3817, 4100
MvnI	CGCG	1 cut(s)	3915
NarI	GGCGCC	2 cut(s)	2356, 2824
NciI	CCSGG	1 cut(s)	4049
NdeI	CATATG	1 cut(s)	2449
NlaIV	GGNNCC	9 cut(s)	774, 796, 1076, 2056, 2357, 2825, 3243, 3609, 3618
NmeAIII	GCCGAG	1 cut(s)	3904
NmuCI	GTSAC	3 cut(s)	3, 73, 757
NsiI	ATGCAT	2 cut(s)	451, 2395
NspI	RCATGY	2 cut(s)	2930, 3380
NspV	TTCGAA	2 cut(s)	607, 2110
OliI	CACNNNNGTG	2 cut(s)	737, 3938
PagI	TCATGA	5 cut(s)	1122, 1500, 2128, 2499, 2892
PauI	GCGCGC	1 cut(s)	3913
PceI	AGGCCT	2 cut(s)	1702, 2170
PciSI	GCTCTTC	1 cut(s)	827
PcsI	WCGNNNNNNNCGW	2 cut(s)	333, 3882
PctI	GAATGC	2 cut(s)	2118, 2984
PdmI	GAANNNNTTC	4 cut(s)	2041, 2218, 2888, 4114
PfeI	GAWTC	7 cut(s)	11, 282, 917, 1247, 1265, 3034, 3159
Pfl23II	CGTACG	1 cut(s)	906
PinAI	ACCGGT	2 cut(s)	1947, 2897
Ple19I	CGATCG	1 cut(s)	2234
PleI	GAGTC	7 cut(s)	220, 754, 1904, 1924, 3448, 3676, 3889
PluTI	GGCGCC	2 cut(s)	2359, 2827
PpsI	GAGTC	7 cut(s)	220, 754, 1904, 1924, 3448, 3676, 3889
PshBI	ATTAAT	1 cut(s)	2514
Psp124BI	GAGCTC	3 cut(s)	188, 3291, 3494
Psp1406I	AACGTT	1 cut(s)	3755
Psp6I	CCWGG	9 cut(s)	576, 1225, 1941, 2913, 3140, 3237, 3612, 3815, 4098
PspFI	CCCAGC	2 cut(s)	1870, 2948
PspGI	CCWGG	9 cut(s)	576, 1225, 1941, 2913, 3140, 3237, 3612, 3815, 4098
PspLI	CGTACG	1 cut(s)	906
PspN4I	GGNNCC	9 cut(s)	774, 796, 1076, 2056, 2357, 2825, 3243, 3609, 3618
PspPI	GGNCC	8 cut(s)	519, 652, 1234, 1945, 2488, 2713, 3196, 3470
PstI	CTGCAG	3 cut(s)	2806, 3033, 3340
PstNI	CAGNNNCTG	2 cut(s)	3344, 3677
PsuI	RGATCY	1 cut(s)	3890
PteI	GCGCGC	1 cut(s)	3913
PvuI	CGATCG	1 cut(s)	2234
PvuII	CAGCTG	2 cut(s)	1193, 2552
RsaI	GTAC	8 cut(s)	455, 557, 908, 2025, 2477, 2522, 2769, 3510
RsaNI	GTAC	8 cut(s)	454, 556, 907, 2024, 2476, 2521, 2768, 3509
RseI	CAYNNNNRTG	4 cut(s)	737, 2111, 3602, 3938
SacI	GAGCTC	3 cut(s)	188, 3291, 3494
SapI	GCTCTTC	1 cut(s)	827
SaqAI	TTAA	9 cut(s)	1491, 1538, 1613, 1677, 1863, 2514, 2534, 3255, 3372
Sau96I	GGNCC	8 cut(s)	519, 652, 1234, 1945, 2488, 2713, 3196, 3470
ScaI	AGTACT	1 cut(s)	2522
SchI	GAGTC	7 cut(s)	221, 754, 1904, 1925, 3448, 3677, 3890
SduI	GDGCHC	8 cut(s)	188, 777, 928, 3291, 3442, 3494, 3666, 3871
SfaNI	GCATC	7 cut(s)	417, 631, 649, 1696, 2281, 2710, 3319
SfcI	CTRYAG	5 cut(s)	867, 879, 2802, 3029, 3336
SfoI	GGCGCC	2 cut(s)	2357, 2825
SfuI	TTCGAA	2 cut(s)	607, 2110
SinI	GGWCC	7 cut(s)	519, 652, 1234, 1945, 2488, 3196, 3470
SmiMI	CAYNNNNRTG	4 cut(s)	737, 2111, 3602, 3938
SmlI	CTYRAG	3 cut(s)	65, 171, 3578
SmoI	CTYRAG	3 cut(s)	65, 171, 3578
SpeI	ACTAGT	1 cut(s)	1279
SseBI	AGGCCT	2 cut(s)	1702, 2170
SsiI	CCGC	7 cut(s)	225, 1237, 1987, 2868, 3298, 3514, 4066
SspDI	GGCGCC	2 cut(s)	2355, 2823
SstI	GAGCTC	3 cut(s)	188, 3291, 3494
StuI	AGGCCT	2 cut(s)	1702, 2170
StyI	CCWWGG	2 cut(s)	2931, 4142
TaiI	ACGT	3 cut(s)	306, 912, 3758
TaqI	TCGA	9 cut(s)	536, 594, 607, 892, 2110, 2230, 3265, 3400, 3852
TaqII	GACCGA	1 cut(s)	1108
TatI	WGTACW	6 cut(s)	453, 2023, 2475, 2520, 2767, 3508
TauI	GCSGC	1 cut(s)	2870
TfiI	GAWTC	7 cut(s)	11, 282, 917, 1247, 1265, 3034, 3159
Tru1I	TTAA	9 cut(s)	1491, 1538, 1613, 1677, 1863, 2514, 2534, 3255, 3372
Tru9I	TTAA	9 cut(s)	1491, 1538, 1613, 1677, 1863, 2514, 2534, 3255, 3372
TseFI	GTSAC	3 cut(s)	3, 73, 757
Tsp45I	GTSAC	3 cut(s)	3, 73, 757
TspGWI	ACGGA	1 cut(s)	3425
VneI	GTGCAC	1 cut(s)	3867
VpaK11BI	GGWCC	7 cut(s)	519, 652, 1234, 1945, 2488, 3196, 3470
VspI	ATTAAT	1 cut(s)	2514
XapI	RAATTY	3 cut(s)	888, 2215, 3774
XbaI	TCTAGA	1 cut(s)	2314
XceI	RCATGY	2 cut(s)	2930, 3380
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1085
XmiI	GTMKAC	1 cut(s)	3381
XmnI	GAANNNNTTC	4 cut(s)	2041, 2218, 2888, 4114
ZrmI	AGTACT	1 cut(s)	2522
Zsp2I	ATGCAT	2 cut(s)	451, 2395

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.