Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0010579

Physical Location & Seq

Genomic Coordinates Forward (+)

17141531 .. 17152965

Gene Structure 11435 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1094300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2013 bp

ATGGTTCAACTTCATGCAACTTTCTCTTTCAGAAACTCTCCCAAATTCTCAGGTTCTTCTCCCAAACGTCCCCGTTTCTCTCCCACATCCTCAGGCCTCTCTCCGAAACGTCACCGTTTCGGTGTCAAATATCTCAGAGCAAATCTCCTTGGCACTAATTTAACATTTAATTGCAAGGCCAATGGAGGAGAATTGGATCCCCTGCCTCACTTGCAGACTCTCGGAACATTTCCAAGGGAGGAGCTTGCTGCAAAAGTTGTCCTGGTCAGATTTGACTCTGCCCTTTTGCTTCATGAACATGTGGAACAACACAGATTCCAATCCGATGCGGTATTAACGATTAAGTATTTATGCCAATCCGGGGCTAGAGTAGTTCTTGCTAGTGATTGGAGTGTTAAAACTAATCCTAAACTGTGTGTTGCACAGTCTGTTGCAGATTTCTTGTCATCTCTTGTTGACTACAAAGTTGCTCCGGTGCAATGTATTTCTAACCTGGCATCAAAGGTGGAAGGTTTTGAGAAAGGAGATATTGTTCTTCTTGAGAATCTTTCTGAGTTTAGAGGGGAAGTTGCCAATTGTTCAAAATTTGCTCAAGTATTGTCATCAGGAGTTGATATTTTTGTTAATGACTACTTTTCCCGGTCTCATAAGATGCTTGCATCCACCTGTGGAGTTGCTCGCTTCTGCTATGGCAACTTAGCTGGTTTTCACTTTGAGAAGAGCCTATCTCAACTTAGAAGGGCTGCAGAAACCGACACAAAACCTTATGTAGCAATTATTGGTGGGGGTAATCTCTCTGATAAAGCAGCTGCTTTGCACTCCTTAGCTTCAATATGCGATGGGTTAGTCTTTGTTGGAATGATGTCATTTCAAATAATTCACGCCTTAGGGATATCTGTTCCCTTGAATTTCTTGGAACATGGGGCACTTAAGGAAGCTTTAGATATAGTCCAGGTTGCACACGATAGAAATGTACAGATACTATATCCAAAAGACTTTTGGTGCAAGAATGATTCTCTTCCGAAGCAATTGGAAATATTTCCTGCTCATGGTATTCCAGATGGATGGGTACCTATTGATATTGGGCCTGTATCGTTGGTTGAAATAAACTCCATGCTTACAAAGTGCAAGAAAGTCACTTGGATTGGTCCAGTAAAATTCTGTATGAGTAACTGCACAAAAGGAGCTTCTATACTGGCCCAAATGCTTAATCAACTAAGTCAAAGCAACTGCAGCGTAACTGTTGTTGGGAGTATGGCATGCGAAGCAATGGTGAAGGAATCAAATTCTGTCTCTCATTTGACCATGATTGAGAATGCTTCAGTTGTATGGGAGTTTCTCAAGGGAAGAAAACTTCCGGGGGTCATGGCCTTAGATAGGGCATACCCTTTTGATATTGACTGGATTGCTGCCTATTCTGATCCAGCTCAACCTTTGGTAGTTGATATTGGAAGTGGTAATGGAATGTTCCTTTTCGGGATGGCCAAAATAAGGAAGGATTTGAACTTTCTTGGTTTGGAGATGAATAAAAAGCTGGTACAACCTTGCCTGGATTCTGTTCACCAGTCTGGTATACGAAACGGGTATTTCATTGCAACAAATGCAACATCAACATTCCGTTCAATTATCTCTAGTTACCCTGGAAAGCTAGTTCTTGTGTCAATACAGTGTCCGAATCCTGATTTCAGTGAACCAGAACATAGATGGAGTATGCTGCAAAGGTCATTAGTTGAAGCAATTTCTGATCTCCTTACAGCTAATGGAAAGGTCTTTCTGCAATCTGACATAGAGACCGTCTCTGTGAGAATGAAAGAACAGTTTCACAAATATGGTAAGGGAAAGCTTACTGTTCTGCATGAACAAAGTTATGTCGTAACTAATGGAGGGTGGCTTAAGGAGAACCCCTTTGGAGTTCGGTCAGATTGGGAACGACATGTCCTAGATCGTGGGGCTCCTATGTACAGATTGATGCTTCGTAAATCAAACTCTGGTGAAAGAAGTGATTGCAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009941 GO:0009987 GO:0010035 GO:0010038 GO:0010319 GO:0015977 GO:0015979 GO:0016020 GO:0016051 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019253 GO:0019538 GO:0019685 GO:0030312 GO:0031967 GO:0031975 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00010 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00710 map01100 map01110 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

Methyltransf_4 PGK

Protein Analysis

671

Amino Acids

74.0

Weight (kDa)

8.75

Isoelectric Point (pI)

35.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PGK	PF00162	82 - 441	9.6e-60	Phosphoglycerate kinase
Methyltransf_4	PF02390	477 - 614	1.4e-22	Putative methyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010579)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g16270 FvH4_6g16270 FvH4_6g16270
malus_domestica	MD04G1094300.v1.1
prunus_persica	Prupe.6G232800_v2.0.a1 Prupe.6G232800_v2.0.a1 Prupe.6G232800_v2.0.a1 Prupe.6G232800_v2.0.a1 Prupe.6G232800_v2.0.a1
pyrus_communis	pycom04g08930
rosa_chinensis	RchiOBHm_Chr3g0470131
rosa_laevigata	RLG00000024294
rosa_roxburghii	Rroxscaffold_6G00411150
rosa_rugosa	Rorug03G0107200 Rorug03G0107300 Rorug03G0107400
rosa_samantha	Rh3BG180400 Rh3CG171200 Rh3DG194600
rosa_wichuraiana	Rw3G014720 Rw3G014810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1069
AccB1I	GGYRCC	1 cut(s)	1069
AccI	GTMKAC	1 cut(s)	1573
AciI	CCGC	1 cut(s)	329
AclWI	GGATC	3 cut(s)	191, 204, 1415
AcoI	YGGCCR	1 cut(s)	1482
AcsI	RAATTY	5 cut(s)	44, 584, 907, 1157, 1285
AcuI	CTGAAG	1 cut(s)	1305
AfaI	GTAC	4 cut(s)	975, 1071, 1539, 1961
AfiI	CCNNNNNNNGG	4 cut(s)	361, 1049, 1377, 1491
AflII	CTTAAG	2 cut(s)	929, 1892
AflIII	ACRYGT	2 cut(s)	298, 1933
AgsI	TTSAA	9 cut(s)	8, 582, 831, 872, 907, 1103, 1504, 1623, 1733
AjnI	CCWGG	5 cut(s)	261, 492, 951, 1548, 1639
AloI	GAACNNNNNNTCC	6 cut(s)	516, 548, 1635, 1667, 1920, 1952
Alw26I	GTCTC	4 cut(s)	648, 1297, 1784, 1801
AlwI	GGATC	3 cut(s)	191, 204, 1415
AoxI	GGCC	6 cut(s)	94, 177, 1085, 1197, 1368, 1482
ApeKI	GCWGC	7 cut(s)	248, 743, 806, 809, 1233, 1409, 1714
ApoI	RAATTY	5 cut(s)	44, 584, 907, 1157, 1285
Asp700I	GAANNNNTTC	2 cut(s)	1038, 1818
Asp718I	GGTACC	1 cut(s)	1069
AspS9I	GGNCC	3 cut(s)	1085, 1148, 1198
AsuC2I	CCSGG	3 cut(s)	361, 640, 1359
AsuHPI	GGTGA	4 cut(s)	104, 1285, 1553, 2003
AvaII	GGWCC	1 cut(s)	1148
AxyI	CCTNAGG	2 cut(s)	91, 886
BaeGI	GKGCMC	1 cut(s)	928
BalI	TGGCCA	1 cut(s)	1484
BamHI	GGATCC	1 cut(s)	196
BanI	GGYRCC	1 cut(s)	1069
BanII	GRGCYC	1 cut(s)	1954
BbvI	GCAGC	7 cut(s)	235, 730, 796, 818, 1245, 1396, 1701
BccI	CCATC	5 cut(s)	833, 1055, 1059, 1474, 1698
BciT130I	CCWGG	5 cut(s)	263, 494, 953, 1550, 1641
BcnI	CCSGG	3 cut(s)	361, 640, 1359
BcoDI	GTCTC	4 cut(s)	648, 1297, 1784, 1801
BfaI	CTAG	5 cut(s)	366, 381, 1632, 1649, 1940
BfmI	CTRYAG	2 cut(s)	744, 1231
BfrI	CTTAAG	2 cut(s)	929, 1892
BisI	GCNGC	7 cut(s)	249, 744, 807, 810, 1234, 1410, 1715
BlsI	GCNGC	7 cut(s)	250, 745, 808, 811, 1235, 1411, 1716
Bme1390I	CCNGG	8 cut(s)	263, 361, 494, 640, 953, 1359, 1550, 1641
Bme18I	GGWCC	1 cut(s)	1148
BmgT120I	GGNCC	3 cut(s)	1085, 1148, 1198
BmiI	GGNNCC	3 cut(s)	198, 1071, 1953
BmrFI	CCNGG	8 cut(s)	263, 361, 494, 640, 953, 1359, 1550, 1641
BmsI	GCATC	5 cut(s)	316, 506, 642, 668, 1959
BplI	GAGNNNNNCTC	6 cut(s)	129, 161, 712, 744, 1781, 1813
Bpu10I	CCTNAGC	1 cut(s)	823
BpuEI	CTTGAG	3 cut(s)	560, 576, 1325
BpuMI	CCSGG	3 cut(s)	361, 640, 1359
BsaBI	GATNNNNATC	1 cut(s)	319
BsaI	GGTCTC	2 cut(s)	648, 1784
BsaJI	CCNNGG	5 cut(s)	148, 233, 360, 1358, 1639
BsaWI	WCCGGW	1 cut(s)	472
BsaXI	ACNNNNNCTCC	2 cut(s)	516, 546
Bsc4I	CCNNNNNNNGG	4 cut(s)	361, 1049, 1377, 1491
Bse1I	ACTGG	4 cut(s)	1151, 1200, 1406, 1564
Bse21I	CCTNAGG	2 cut(s)	91, 886
Bse3DI	GCAATG	3 cut(s)	485, 1275, 1590
Bse8I	GATNNNNATC	1 cut(s)	319
BseBI	CCWGG	5 cut(s)	263, 494, 953, 1550, 1641
BseDI	CCNNGG	5 cut(s)	148, 233, 360, 1358, 1639
BseGI	GGATG	4 cut(s)	86, 659, 1070, 1485
BseJI	GATNNNNATC	1 cut(s)	319
BseLI	CCNNNNNNNGG	4 cut(s)	361, 1049, 1377, 1491
BseMI	GCAATG	3 cut(s)	485, 1275, 1590
BseMII	CTCAG	4 cut(s)	63, 105, 148, 543
BseNI	ACTGG	4 cut(s)	1151, 1200, 1406, 1564
BseRI	GAGGAG	2 cut(s)	201, 254
BseSI	GKGCMC	1 cut(s)	928
BseXI	GCAGC	7 cut(s)	235, 730, 796, 818, 1245, 1396, 1701
BsgI	GTGCAG	1 cut(s)	1159
BshFI	GGCC	6 cut(s)	96, 179, 1087, 1199, 1370, 1484
BshNI	GGYRCC	1 cut(s)	1069
BsiSI	CCGG	4 cut(s)	360, 473, 640, 1358
BslFI	GGGAC	1 cut(s)	54
BslI	CCNNNNNNNGG	4 cut(s)	361, 1049, 1377, 1491
BsmAI	GTCTC	4 cut(s)	648, 1297, 1784, 1801
BsmBI	CGTCTC	1 cut(s)	1801
BsmFI	GGGAC	1 cut(s)	54
BsmI	GAATGC	1 cut(s)	1321
BsnI	GGCC	6 cut(s)	96, 179, 1087, 1199, 1370, 1484
Bso31I	GGTCTC	2 cut(s)	648, 1784
Bsp1286I	GDGCHC	2 cut(s)	928, 1954
Bsp1407I	TGTACA	2 cut(s)	973, 1959
Bsp143I	GATC	4 cut(s)	196, 1420, 1744, 1942
BspACI	CCGC	1 cut(s)	329
BspANI	GGCC	6 cut(s)	96, 179, 1087, 1199, 1370, 1484
BspCNI	CTCAG	4 cut(s)	62, 104, 147, 544
BspHI	TCATGA	1 cut(s)	292
BspLI	GGNNCC	3 cut(s)	198, 1071, 1953
BspMAI	CTGCAG	2 cut(s)	748, 1235
BspPI	GGATC	3 cut(s)	191, 204, 1415
BspQI	GCTCTTC	1 cut(s)	713
BspT107I	GGYRCC	1 cut(s)	1069
BspTI	CTTAAG	2 cut(s)	929, 1892
BspTNI	GGTCTC	2 cut(s)	648, 1784
BsrDI	GCAATG	3 cut(s)	485, 1275, 1590
BsrGI	TGTACA	2 cut(s)	973, 1959
BsrI	ACTGG	4 cut(s)	1151, 1200, 1406, 1564
BssECI	CCNNGG	5 cut(s)	148, 233, 360, 1358, 1639
BssMI	GATC	4 cut(s)	196, 1420, 1744, 1942
BssNAI	GTATAC	1 cut(s)	1574
BssT1I	CCWWGG	2 cut(s)	148, 233
Bst1107I	GTATAC	1 cut(s)	1574
Bst2UI	CCWGG	5 cut(s)	263, 494, 953, 1550, 1641
Bst4CI	ACNGT	8 cut(s)	116, 414, 426, 1243, 1668, 1795, 1818, 1849
Bst6I	CTCTTC	2 cut(s)	713, 1023
BstAFI	CTTAAG	2 cut(s)	929, 1892
BstAPI	GCANNNNNTGC	1 cut(s)	1601
BstAUI	TGTACA	2 cut(s)	973, 1959
BstC8I	GCNNGC	4 cut(s)	246, 657, 679, 1261
BstENI	CCTNNNNNAGG	1 cut(s)	1375
BstF5I	GGATG	4 cut(s)	86, 659, 1070, 1485
BstKTI	GATC	4 cut(s)	199, 1423, 1747, 1945
BstMAI	GTCTC	4 cut(s)	648, 1297, 1784, 1801
BstMBI	GATC	4 cut(s)	196, 1420, 1744, 1942
BstMWI	GCNNNNNNNGC	4 cut(s)	211, 1233, 1265, 1601
BstNI	CCWGG	5 cut(s)	263, 494, 953, 1550, 1641
BstNSI	RCATGY	3 cut(s)	302, 1263, 1937
BstSCI	CCNGG	8 cut(s)	261, 359, 492, 638, 951, 1357, 1548, 1639
BstSFI	CTRYAG	2 cut(s)	744, 1231
BstSLI	GKGCMC	1 cut(s)	928
BstV1I	GCAGC	7 cut(s)	235, 730, 796, 818, 1245, 1396, 1701
BstX2I	RGATCY	1 cut(s)	196
BstXI	CCANNNNNNTGG	1 cut(s)	1065
BstYI	RGATCY	1 cut(s)	196
BstZ17I	GTATAC	1 cut(s)	1574
Bsu36I	CCTNAGG	2 cut(s)	91, 886
BsuRI	GGCC	6 cut(s)	96, 179, 1087, 1199, 1370, 1484
BtgZI	GCGATG	1 cut(s)	852
BtsCI	GGATG	4 cut(s)	86, 659, 1070, 1485
BtsIMutI	CAGTG	2 cut(s)	1673, 1693
Cac8I	GCNNGC	4 cut(s)	246, 657, 679, 1261
CciI	TCATGA	1 cut(s)	292
Cfr13I	GGNCC	3 cut(s)	1085, 1148, 1198
Csp6I	GTAC	4 cut(s)	974, 1070, 1538, 1960
CviQI	GTAC	4 cut(s)	974, 1070, 1538, 1960
DpnI	GATC	4 cut(s)	198, 1422, 1746, 1944
DpnII	GATC	4 cut(s)	196, 1420, 1744, 1942
EaeI	YGGCCR	1 cut(s)	1482
Eam1104I	CTCTTC	2 cut(s)	713, 1023
EarI	CTCTTC	2 cut(s)	713, 1023
Eco130I	CCWWGG	2 cut(s)	148, 233
Eco147I	AGGCCT	1 cut(s)	96
Eco24I	GRGCYC	1 cut(s)	1954
Eco31I	GGTCTC	2 cut(s)	648, 1784
Eco32I	GATATC	1 cut(s)	894
Eco47I	GGWCC	1 cut(s)	1148
Eco57I	CTGAAG	1 cut(s)	1305
Eco81I	CCTNAGG	2 cut(s)	91, 886
EcoNI	CCTNNNNNAGG	1 cut(s)	1375
EcoRII	CCWGG	5 cut(s)	261, 492, 951, 1548, 1639
EcoRV	GATATC	1 cut(s)	894
EcoT14I	CCWWGG	2 cut(s)	148, 233
EcoT38I	GRGCYC	1 cut(s)	1954
ErhI	CCWWGG	2 cut(s)	148, 233
Esp3I	CGTCTC	1 cut(s)	1801
FaqI	GGGAC	1 cut(s)	54
FblI	GTMKAC	1 cut(s)	1573
Fnu4HI	GCNGC	7 cut(s)	249, 744, 807, 810, 1234, 1410, 1715
FokI	GGATG	4 cut(s)	73, 646, 1077, 1492
FriOI	GRGCYC	1 cut(s)	1954
Fsp4HI	GCNGC	7 cut(s)	249, 744, 807, 810, 1234, 1410, 1715
FspBI	CTAG	5 cut(s)	366, 381, 1632, 1649, 1940
GluI	GCNGC	7 cut(s)	249, 744, 807, 810, 1234, 1410, 1715
HaeIII	GGCC	6 cut(s)	96, 179, 1087, 1199, 1370, 1484
HapII	CCGG	4 cut(s)	360, 473, 640, 1358
HincII	GTYRAC	1 cut(s)	457
HindII	GTYRAC	1 cut(s)	457
HindIII	AAGCTT	2 cut(s)	936, 1841
HinfI	GANTC	8 cut(s)	217, 275, 315, 544, 1013, 1280, 1553, 1675
HpaII	CCGG	4 cut(s)	360, 473, 640, 1358
HphI	GGTGA	4 cut(s)	104, 1285, 1553, 2003
Hpy166II	GTNNAC	4 cut(s)	457, 1561, 1574, 1691
Hpy188III	TCNNGA	6 cut(s)	293, 539, 606, 1058, 1477, 1679
Hpy8I	GTNNAC	4 cut(s)	457, 1561, 1574, 1691
HpyAV	CCTTC	4 cut(s)	503, 732, 1270, 1489
HpyCH4III	ACNGT	8 cut(s)	116, 414, 426, 1243, 1668, 1795, 1818, 1849
HpyCH4IV	ACGT	2 cut(s)	67, 109
HpyF10VI	GCNNNNNNNGC	4 cut(s)	211, 1233, 1265, 1601
HpySE526I	ACGT	2 cut(s)	67, 109
KpnI	GGTACC	1 cut(s)	1073
Kzo9I	GATC	4 cut(s)	196, 1420, 1744, 1942
LguI	GCTCTTC	1 cut(s)	713
LmnI	GCTCC	4 cut(s)	241, 475, 1184, 1957
Lsp1109I	GCAGC	7 cut(s)	235, 730, 796, 818, 1245, 1396, 1701
LweI	GCATC	5 cut(s)	316, 506, 642, 668, 1959
MaeI	CTAG	5 cut(s)	366, 381, 1632, 1649, 1940
MaeII	ACGT	2 cut(s)	67, 109
MaeIII	GTNAC	6 cut(s)	110, 1135, 1169, 1237, 1634, 1873
MalI	GATC	4 cut(s)	198, 1422, 1746, 1944
MboI	GATC	4 cut(s)	196, 1420, 1744, 1942
MboII	GAAGA	5 cut(s)	48, 527, 730, 1010, 1359
MfeI	CAATTG	2 cut(s)	574, 1028
MflI	RGATCY	1 cut(s)	196
MhlI	GDGCHC	2 cut(s)	928, 1954
MlsI	TGGCCA	1 cut(s)	1484
MluNI	TGGCCA	1 cut(s)	1484
MlyI	GAGTC	2 cut(s)	211, 269
MmeI	TCCRAC	1 cut(s)	835
MnlI	CCTC	7 cut(s)	100, 107, 179, 216, 232, 554, 1877
Mox20I	TGGCCA	1 cut(s)	1484
MroXI	GAANNNNTTC	2 cut(s)	1038, 1818
MscI	TGGCCA	1 cut(s)	1484
MslI	CAYNNNNRTG	2 cut(s)	297, 1827
Msp20I	TGGCCA	1 cut(s)	1484
MspA1I	CMGCKG	1 cut(s)	809
MspCI	CTTAAG	2 cut(s)	929, 1892
MspI	CCGG	4 cut(s)	360, 473, 640, 1358
MspR9I	CCNGG	8 cut(s)	263, 361, 494, 640, 953, 1359, 1550, 1641
MunI	CAATTG	2 cut(s)	574, 1028
Mva1269I	GAATGC	1 cut(s)	1321
MvaI	CCWGG	5 cut(s)	263, 494, 953, 1550, 1641
MwoI	GCNNNNNNNGC	4 cut(s)	211, 1233, 1265, 1601
NciI	CCSGG	3 cut(s)	361, 640, 1359
NdeII	GATC	4 cut(s)	196, 1420, 1744, 1942
NlaIV	GGNNCC	3 cut(s)	198, 1071, 1953
NmuCI	GTSAC	2 cut(s)	110, 1135
NspI	RCATGY	3 cut(s)	302, 1263, 1937
PaeI	GCATGC	1 cut(s)	1263
PagI	TCATGA	1 cut(s)	292
PceI	AGGCCT	1 cut(s)	96
PciI	ACATGT	2 cut(s)	298, 1933
PciSI	GCTCTTC	1 cut(s)	713
PctI	GAATGC	1 cut(s)	1321
PdmI	GAANNNNTTC	2 cut(s)	1038, 1818
PfeI	GAWTC	6 cut(s)	315, 544, 1013, 1280, 1553, 1675
PkrI	GCNGC	7 cut(s)	250, 745, 808, 811, 1235, 1411, 1716
PleI	GAGTC	2 cut(s)	211, 269
PpsI	GAGTC	2 cut(s)	211, 269
PscI	ACATGT	2 cut(s)	298, 1933
Psp6I	CCWGG	5 cut(s)	261, 492, 951, 1548, 1639
PspGI	CCWGG	5 cut(s)	261, 492, 951, 1548, 1639
PspN4I	GGNNCC	3 cut(s)	198, 1071, 1953
PspPI	GGNCC	3 cut(s)	1085, 1148, 1198
PstI	CTGCAG	2 cut(s)	748, 1235
PsuI	RGATCY	1 cut(s)	196
PvuII	CAGCTG	1 cut(s)	809
RsaI	GTAC	4 cut(s)	975, 1071, 1539, 1961
RsaNI	GTAC	4 cut(s)	974, 1070, 1538, 1960
RseI	CAYNNNNRTG	2 cut(s)	297, 1827
SapI	GCTCTTC	1 cut(s)	713
SatI	GCNGC	7 cut(s)	249, 744, 807, 810, 1234, 1410, 1715
Sau3AI	GATC	4 cut(s)	196, 1420, 1744, 1942
Sau96I	GGNCC	3 cut(s)	1085, 1148, 1198
SchI	GAGTC	2 cut(s)	211, 269
ScrFI	CCNGG	8 cut(s)	263, 361, 494, 640, 953, 1359, 1550, 1641
SduI	GDGCHC	2 cut(s)	928, 1954
SfaNI	GCATC	5 cut(s)	316, 506, 642, 668, 1959
SfcI	CTRYAG	2 cut(s)	744, 1231
SinI	GGWCC	1 cut(s)	1148
SmiMI	CAYNNNNRTG	2 cut(s)	297, 1827
SmlI	CTYRAG	5 cut(s)	539, 591, 929, 1340, 1892
SmoI	CTYRAG	5 cut(s)	539, 591, 929, 1340, 1892
SphI	GCATGC	1 cut(s)	1263
SseBI	AGGCCT	1 cut(s)	96
SsiI	CCGC	1 cut(s)	329
SspI	AATATT	1 cut(s)	1038
SspMI	CTAG	5 cut(s)	366, 381, 1632, 1649, 1940
StuI	AGGCCT	1 cut(s)	96
StyD4I	CCNGG	8 cut(s)	261, 359, 492, 638, 951, 1357, 1548, 1639
StyI	CCWWGG	2 cut(s)	148, 233
TaaI	ACNGT	8 cut(s)	116, 414, 426, 1243, 1668, 1795, 1818, 1849
TaiI	ACGT	2 cut(s)	70, 112
TaqII	GACCGA	1 cut(s)	1905
TatI	WGTACW	2 cut(s)	973, 1959
TfiI	GAWTC	6 cut(s)	315, 544, 1013, 1280, 1553, 1675
TscAI	CASTG	2 cut(s)	1673, 1693
TseFI	GTSAC	2 cut(s)	110, 1135
TseI	GCWGC	7 cut(s)	248, 743, 806, 809, 1233, 1409, 1714
Tsp45I	GTSAC	2 cut(s)	110, 1135
TspDTI	ATGAA	6 cut(s)	281, 309, 1538, 1579, 1823, 1872
TspGWI	ACGGA	1 cut(s)	1607
TspRI	CASTG	2 cut(s)	1673, 1693
Vha464I	CTTAAG	2 cut(s)	929, 1892
VpaK11BI	GGWCC	1 cut(s)	1148
XagI	CCTNNNNNAGG	1 cut(s)	1375
XapI	RAATTY	5 cut(s)	44, 584, 907, 1157, 1285
XceI	RCATGY	3 cut(s)	302, 1263, 1937
XcmI	CCANNNNNNNNNTGG	2 cut(s)	996, 1701
XmiI	GTMKAC	1 cut(s)	1573
XmnI	GAANNNNTTC	2 cut(s)	1038, 1818
XspI	CTAG	5 cut(s)	366, 381, 1632, 1649, 1940

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.