Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0010579

Physical Location & Seq

Genomic Coordinates Forward (+)

17462621 .. 17468193

Gene Structure 5573 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG194600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1929 bp

ATGGGTCTACTTGTGAATCTTCTTCACATTTCTGCATTTTCATCCATTGCTTATAAACCTCACAGTGTTGAATATCCCAAAGCAAATGTAACAATTAAATGTAAGGCCTATGGAGGAGAATTGGATGCTTTGCCTCACTTACAAACTCTTGGAAAATTTCCAAGGGAGGAGCTTGTTGAAAAAGTTGTCCTGGTCAGATTTGACTCTACCCTTTTGCTTCAGGAACAAGAAAAAGAACAAAGATTTCAATCCAATGCTGTTTTGACCATTAAGTATTTACACCAGTCCGGGGCTAAAGTAGTTCTTGTGAGTGACTGGAGTGCTAAAACTAATCCTAAAGTGTTTGCTGCACAGTCTGTTGCAGGTTTCTTGTCATCAATTCTTGACTACAAAGTTGTTCCGGTACGATGCATTTCTCATAACACGGTATCAAAGACAGAAGGCTTTGAAAAAGCAGATATTGTTCTCCTTGAGAATCTTTCTGGTTTTAGAGGGGAAGTTGCCAATTGTTCAAAATTTGCTCAAGTTTTGTCATCGGGAGTTGATATCTTTGTTAATGACTACTTTTCCCGGTCTCATAAGATTCTTGCATCGACCTGTGGAGTTGCTCGTTTCTGTTATGCTTGTTTAGCTGGTTTTCACTTTGAGGAGTGCCTATATCACCTCAGAAGGCTTGCAGAAACCAGTACAAAACCTTATGTAGCAATTATTGGAGGCAGTAATCTTTCTGATAAAGCCGATGCTGTGCATACCTTAGCTTCTATATGTGATGGGTTAGTGTTTGTTGGAATGATGTCATTCCAAATAATGCATGCATTAGGGCTATCTGTTCCCTTGAATTTGGTGGAACATGGAGCACTTAAAGAAGCTTTAGATATAGTCCAGTTTGCACACAATAGAAATGTACAGATCCTATATCCAAAAGACTTTTGGTGCAAGAATGATCGTCATCCGAAGCAATTGGAAATATATCCTGCACATGCTATTCCGGATGGGTGGGTACCTGTTGATATTGGGCCTGTATCGTTAGATGGAATAAACTCCATTCTCACTAAATGCAAGAAAGTTACCTGGATTGGTCCAGTGAAATTTCATACGAACAGCGGCACAAAAGGAGCTTCTAAAGTGGCGCAATTGCTTAATCAACTAAGTCAAGGCAACAGCAACATAACTGTCATTGGCAATATGGCATGTCAAGCAATGGTGAAGGAATCAAATTCCATCTTTCACTTCAATATGTTAGAGAATGCTTCAGTTGTATGGGAGTTTCTCAAAGGAAGAAAACTTCCAGGCATCATGGCTTTAGATAGAGCATTCCCTTTTGATATTGACTGGAAGGTAGCCTATTCAGATCCTGCTCAACCTTTGGTGGTTGATATTGGAAGTGGTAATGGAATGTTTCTTTTCGGGATGGCTAAAACAAGGAAGGATTTGAATTTTCTCGGTTTGGAGATTAATAAAAAGCTGGTAAAACGTTGCCTGGATTCCAGTCATCAGTGTGGTATAAGAAATGGGTACTTCATCGCAACAAATGCAACATCAACATTCCGCTCCATAGTTTCTAGTTACCCCGGAAAGCTAGTACTTGTGTCAATACAGTGTCCAAATCCTGATTTCAACGAACCAGAACATCGATGGAGAATGCTGCAAAGGTCATTAGTCGAAGCAGTTGCTGATATCCTTACAGTTGATGGAAAGGTGTTTCTGCAATCTGATATAGAGGTTGTCTCTATGAGAATGAAGGAACAGTTTCTGAGACATGGCAAGGGCAAACTTACTATTGTGCATGAGCAAAGTTATGCCATAAATAATGAAGGGTGGCTCAAGGACAATCCATTCGGAGTTCGGTCAGACTGGGAACAACATGTCTTAGATCGTGGGGCCCCTATGTACAGATTAATGCTTTGTAAATCAGCCTCAAGTGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009941 GO:0009987 GO:0010035 GO:0010038 GO:0010319 GO:0015977 GO:0015979 GO:0016020 GO:0016051 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019253 GO:0019538 GO:0019685 GO:0030312 GO:0031967 GO:0031975 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00010 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00710 map01100 map01110 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

Methyltransf_4 PGK

Protein Analysis

642

Amino Acids

71.41

Weight (kDa)

8.64

Isoelectric Point (pI)

35.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PGK	PF00162	58 - 406	5.5e-59	Phosphoglycerate kinase
Methyltransf_4	PF02390	454 - 604	5.6e-25	Putative methyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010579)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g16270 FvH4_6g16270 FvH4_6g16270
malus_domestica	MD04G1094300.v1.1
prunus_persica	Prupe.6G232800_v2.0.a1 Prupe.6G232800_v2.0.a1 Prupe.6G232800_v2.0.a1 Prupe.6G232800_v2.0.a1 Prupe.6G232800_v2.0.a1
pyrus_communis	pycom04g08930
rosa_chinensis	RchiOBHm_Chr3g0470131
rosa_laevigata	RLG00000024294
rosa_roxburghii	Rroxscaffold_6G00411150
rosa_rugosa	Rorug03G0107200 Rorug03G0107300 Rorug03G0107400
rosa_samantha	Rh3BG180400 Rh3CG171200 Rh3DG194600
rosa_wichuraiana	Rw3G014720 Rw3G014810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	54
Acc36I	ACCTGC	1 cut(s)	353
Acc65I	GGTACC	1 cut(s)	1000
AccB1I	GGYRCC	1 cut(s)	1000
AccBSI	CCGCTC	1 cut(s)	1551
AccI	GTMKAC	1 cut(s)	7
AccIII	TCCGGA	1 cut(s)	988
AciI	CCGC	2 cut(s)	1104, 1549
AclI	AACGTT	1 cut(s)	1474
AclWI	GGATC	2 cut(s)	904, 1346
AcsI	RAATTY	6 cut(s)	155, 515, 838, 1088, 1216, 1435
AcuI	CTGAAG	2 cut(s)	203, 1236
AfaI	GTAC	7 cut(s)	405, 688, 906, 1002, 1517, 1584, 1892
AfiI	CCNNNNNNNGG	1 cut(s)	289
AflIII	ACRYGT	1 cut(s)	1864
AgsI	TTSAA	9 cut(s)	71, 179, 248, 449, 513, 838, 1234, 1435, 1618
AjnI	CCWGG	4 cut(s)	189, 1070, 1288, 1479
AluBI	AGCT	7 cut(s)	172, 632, 758, 869, 1118, 1465, 1579
AluI	AGCT	7 cut(s)	172, 632, 758, 869, 1118, 1465, 1579
Alw21I	GWGCWC	1 cut(s)	859
Alw26I	GTCTC	3 cut(s)	579, 1732, 1750
AlwI	GGATC	2 cut(s)	904, 1346
AlwNI	CAGNNNCTG	3 cut(s)	1355, 1673, 1753
Aor13HI	TCCGGA	1 cut(s)	988
AoxI	GGCC	3 cut(s)	105, 1016, 1881
ApaI	GGGCCC	1 cut(s)	1885
ApeKI	GCWGC	2 cut(s)	347, 1645
ApoI	RAATTY	6 cut(s)	155, 515, 838, 1088, 1216, 1435
ArsI	GACNNNNNNTTYG	2 cut(s)	1820, 1852
AseI	ATTAAT	2 cut(s)	1455, 1898
Asp700I	GAANNNNTTC	1 cut(s)	1749
Asp718I	GGTACC	1 cut(s)	1000
AspLEI	GCGC	1 cut(s)	1132
AspS9I	GGNCC	4 cut(s)	1016, 1079, 1881, 1882
AsuC2I	CCSGG	3 cut(s)	289, 571, 1572
AsuHPI	GGTGA	2 cut(s)	653, 1216
AvaII	GGWCC	1 cut(s)	1079
BaeGI	GKGCMC	1 cut(s)	1885
BanI	GGYRCC	1 cut(s)	1000
BanII	GRGCYC	1 cut(s)	1885
Bbv12I	GWGCWC	1 cut(s)	859
BbvI	GCAGC	2 cut(s)	334, 1632
BccI	CCATC	7 cut(s)	764, 986, 1025, 1229, 1405, 1629, 1685
BcgI	CGANNNNNNTGC	2 cut(s)	1652, 1686
BciT130I	CCWGG	4 cut(s)	191, 1072, 1290, 1481
BcnI	CCSGG	3 cut(s)	289, 571, 1572
BcoDI	GTCTC	3 cut(s)	579, 1732, 1750
BfaI	CTAG	2 cut(s)	1563, 1580
BfuAI	ACCTGC	1 cut(s)	353
BisI	GCNGC	3 cut(s)	348, 1105, 1646
BlsI	GCNGC	3 cut(s)	349, 1106, 1647
BmcAI	AGTACT	1 cut(s)	1584
Bme1390I	CCNGG	7 cut(s)	191, 289, 571, 1072, 1290, 1481, 1572
Bme18I	GGWCC	1 cut(s)	1079
BmgT120I	GGNCC	4 cut(s)	1016, 1079, 1881, 1882
BmiI	GGNNCC	4 cut(s)	1002, 1882, 1883, 1884
BmrFI	CCNGG	7 cut(s)	191, 289, 571, 1072, 1290, 1481, 1572
BmrI	ACTGGG	1 cut(s)	1864
BmsI	GCATC	5 cut(s)	115, 398, 599, 730, 1302
BmuI	ACTGGG	1 cut(s)	1864
BplI	GAGNNNNNCTC	2 cut(s)	1712, 1744
BpmI	CTGGAG	1 cut(s)	337
Bpu10I	CCTNAGC	1 cut(s)	754
BpuEI	CTTGAG	4 cut(s)	491, 507, 1808, 1903
BpuMI	CCSGG	3 cut(s)	289, 571, 1572
Bsa29I	ATCGAT	1 cut(s)	1633
BsaBI	GATNNNNATC	2 cut(s)	247, 948
BsaI	GGTCTC	1 cut(s)	579
BsaJI	CCNNGG	3 cut(s)	161, 288, 1570
BsaWI	WCCGGW	2 cut(s)	400, 988
Bsc4I	CCNNNNNNNGG	1 cut(s)	289
Bse1I	ACTGG	8 cut(s)	283, 320, 684, 883, 1082, 1337, 1488, 1859
Bse3DI	GCAATG	2 cut(s)	45, 1206
Bse8I	GATNNNNATC	2 cut(s)	247, 948
BseAI	TCCGGA	1 cut(s)	988
BseBI	CCWGG	4 cut(s)	191, 1072, 1290, 1481
BseCI	ATCGAT	1 cut(s)	1633
BseDI	CCNNGG	3 cut(s)	161, 288, 1570
BseGI	GGATG	5 cut(s)	41, 130, 949, 997, 1416
BseJI	GATNNNNATC	2 cut(s)	247, 948
BseLI	CCNNNNNNNGG	1 cut(s)	289
BseMI	GCAATG	2 cut(s)	45, 1206
BseMII	CTCAG	2 cut(s)	679, 1745
BseNI	ACTGG	8 cut(s)	283, 320, 684, 883, 1082, 1337, 1488, 1859
BseRI	GAGGAG	3 cut(s)	129, 182, 662
BseSI	GKGCMC	1 cut(s)	1885
BseXI	GCAGC	2 cut(s)	334, 1632
BsgI	GTGCAG	2 cut(s)	333, 960
BshFI	GGCC	3 cut(s)	107, 1018, 1883
BshNI	GGYRCC	1 cut(s)	1000
BshVI	ATCGAT	1 cut(s)	1633
BsiHKAI	GWGCWC	1 cut(s)	859
BsiSI	CCGG	5 cut(s)	288, 401, 571, 989, 1572
BslI	CCNNNNNNNGG	1 cut(s)	289
BsmAI	GTCTC	3 cut(s)	579, 1732, 1750
BsmI	GAATGC	3 cut(s)	1252, 1313, 1647
BsnI	GGCC	3 cut(s)	107, 1018, 1883
Bso31I	GGTCTC	1 cut(s)	579
Bsp120I	GGGCCC	1 cut(s)	1881
Bsp1286I	GDGCHC	2 cut(s)	859, 1885
Bsp13I	TCCGGA	1 cut(s)	988
Bsp1407I	TGTACA	2 cut(s)	904, 1890
Bsp143I	GATC	4 cut(s)	909, 943, 1351, 1873
BspACI	CCGC	2 cut(s)	1104, 1549
BspANI	GGCC	3 cut(s)	107, 1018, 1883
BspCNI	CTCAG	2 cut(s)	678, 1746
BspDI	ATCGAT	1 cut(s)	1633
BspEI	TCCGGA	1 cut(s)	988
BspLI	GGNNCC	4 cut(s)	1002, 1882, 1883, 1884
BspMI	ACCTGC	1 cut(s)	353
BspPI	GGATC	2 cut(s)	904, 1346
BspT107I	GGYRCC	1 cut(s)	1000
BspTNI	GGTCTC	1 cut(s)	579
BsrBI	CCGCTC	1 cut(s)	1551
BsrDI	GCAATG	2 cut(s)	45, 1206
BsrGI	TGTACA	2 cut(s)	904, 1890
BsrI	ACTGG	8 cut(s)	283, 320, 684, 883, 1082, 1337, 1488, 1859
BssECI	CCNNGG	3 cut(s)	161, 288, 1570
BssMI	GATC	4 cut(s)	909, 943, 1351, 1873
BssT1I	CCWWGG	1 cut(s)	161
Bst2UI	CCWGG	4 cut(s)	191, 1072, 1290, 1481
Bst4CI	ACNGT	7 cut(s)	65, 354, 427, 1174, 1599, 1687, 1749
BstAPI	GCANNNNNTGC	1 cut(s)	1532
BstAUI	TGTACA	2 cut(s)	904, 1890
BstC8I	GCNNGC	2 cut(s)	675, 813
BstDEI	CTNAG	5 cut(s)	665, 754, 1148, 1754, 1870
BstF5I	GGATG	5 cut(s)	41, 130, 949, 997, 1416
BstHHI	GCGC	1 cut(s)	1132
BstKTI	GATC	4 cut(s)	912, 946, 1354, 1876
BstMAI	GTCTC	3 cut(s)	579, 1732, 1750
BstMBI	GATC	4 cut(s)	909, 943, 1351, 1873
BstMWI	GCNNNNNNNGC	3 cut(s)	629, 1196, 1532
BstNI	CCWGG	4 cut(s)	191, 1072, 1290, 1481
BstNSI	RCATGY	4 cut(s)	815, 983, 1194, 1868
BstSCI	CCNGG	7 cut(s)	189, 287, 569, 1070, 1288, 1479, 1570
BstSLI	GKGCMC	1 cut(s)	1885
BstV1I	GCAGC	2 cut(s)	334, 1632
BstX2I	RGATCY	2 cut(s)	909, 1351
BstYI	RGATCY	2 cut(s)	909, 1351
Bsu15I	ATCGAT	1 cut(s)	1633
BsuRI	GGCC	3 cut(s)	107, 1018, 1883
BsuTUI	ATCGAT	1 cut(s)	1633
BtgZI	GCGATG	1 cut(s)	1507
BtsCI	GGATG	5 cut(s)	41, 130, 949, 997, 1416
BtsIMutI	CAGTG	4 cut(s)	70, 1089, 1502, 1604
BveI	ACCTGC	1 cut(s)	353
Cac8I	GCNNGC	2 cut(s)	675, 813
CaiI	CAGNNNCTG	3 cut(s)	1355, 1673, 1753
CfoI	GCGC	1 cut(s)	1132
Cfr13I	GGNCC	4 cut(s)	1016, 1079, 1881, 1882
ClaI	ATCGAT	1 cut(s)	1633
Csp6I	GTAC	7 cut(s)	404, 687, 905, 1001, 1516, 1583, 1891
CviAII	CATG	8 cut(s)	812, 851, 980, 1191, 1297, 1760, 1787, 1865
CviQI	GTAC	7 cut(s)	404, 687, 905, 1001, 1516, 1583, 1891
DdeI	CTNAG	5 cut(s)	665, 754, 1148, 1754, 1870
DpnI	GATC	4 cut(s)	911, 945, 1353, 1875
DpnII	GATC	4 cut(s)	909, 943, 1351, 1873
Eco130I	CCWWGG	1 cut(s)	161
Eco147I	AGGCCT	1 cut(s)	107
Eco24I	GRGCYC	1 cut(s)	1885
Eco31I	GGTCTC	1 cut(s)	579
Eco32I	GATATC	2 cut(s)	547, 1678
Eco47I	GGWCC	1 cut(s)	1079
Eco57I	CTGAAG	2 cut(s)	203, 1236
EcoO109I	RGGNCCY	2 cut(s)	1881, 1882
EcoRII	CCWGG	4 cut(s)	189, 1070, 1288, 1479
EcoRV	GATATC	2 cut(s)	547, 1678
EcoT14I	CCWWGG	1 cut(s)	161
EcoT22I	ATGCAT	3 cut(s)	413, 813, 817
EcoT38I	GRGCYC	1 cut(s)	1885
ErhI	CCWWGG	1 cut(s)	161
FaeI	CATG	8 cut(s)	815, 854, 983, 1194, 1300, 1763, 1790, 1868
FalI	AAGNNNNNCTT	2 cut(s)	288, 320
FatI	CATG	8 cut(s)	811, 850, 979, 1190, 1296, 1759, 1786, 1864
FblI	GTMKAC	1 cut(s)	7
Fnu4HI	GCNGC	3 cut(s)	348, 1105, 1646
FokI	GGATG	5 cut(s)	28, 137, 936, 1004, 1423
FriOI	GRGCYC	1 cut(s)	1885
Fsp4HI	GCNGC	3 cut(s)	348, 1105, 1646
FspBI	CTAG	2 cut(s)	1563, 1580
GlaI	GCGC	1 cut(s)	1131
GluI	GCNGC	3 cut(s)	348, 1105, 1646
GsuI	CTGGAG	1 cut(s)	337
HaeIII	GGCC	3 cut(s)	107, 1018, 1883
HapII	CCGG	5 cut(s)	288, 401, 571, 989, 1572
HhaI	GCGC	1 cut(s)	1132
Hin1II	CATG	8 cut(s)	815, 854, 983, 1194, 1300, 1763, 1790, 1868
Hin6I	GCGC	1 cut(s)	1130
HinP1I	GCGC	1 cut(s)	1130
HindIII	AAGCTT	1 cut(s)	867
HinfI	GANTC	6 cut(s)	16, 203, 475, 583, 1211, 1484
HpaII	CCGG	5 cut(s)	288, 401, 571, 989, 1572
HphI	GGTGA	2 cut(s)	653, 1216
Hpy166II	GTNNAC	1 cut(s)	8
Hpy188I	TCNGA	9 cut(s)	197, 668, 730, 954, 1351, 1714, 1755, 1841, 1852
Hpy188III	TCNNGA	6 cut(s)	221, 383, 537, 989, 1408, 1610
Hpy8I	GTNNAC	1 cut(s)	8
HpyAV	CCTTC	7 cut(s)	434, 663, 1201, 1330, 1420, 1735, 1808
HpyCH4III	ACNGT	7 cut(s)	65, 354, 427, 1174, 1599, 1687, 1749
HpyCH4IV	ACGT	1 cut(s)	1474
HpyF10VI	GCNNNNNNNGC	3 cut(s)	629, 1196, 1532
HpyF3I	CTNAG	5 cut(s)	665, 754, 1148, 1754, 1870
HpySE526I	ACGT	1 cut(s)	1474
Hsp92II	CATG	8 cut(s)	815, 854, 983, 1194, 1300, 1763, 1790, 1868
HspAI	GCGC	1 cut(s)	1130
Kpn2I	TCCGGA	1 cut(s)	988
KpnI	GGTACC	1 cut(s)	1004
Kzo9I	GATC	4 cut(s)	909, 943, 1351, 1873
LmnI	GCTCC	4 cut(s)	169, 854, 1115, 1556
Lsp1109I	GCAGC	2 cut(s)	334, 1632
LweI	GCATC	5 cut(s)	115, 398, 599, 730, 1302
MaeI	CTAG	2 cut(s)	1563, 1580
MaeII	ACGT	1 cut(s)	1474
MaeIII	GTNAC	4 cut(s)	88, 311, 1066, 1565
MalI	GATC	4 cut(s)	911, 945, 1353, 1875
MbiI	CCGCTC	1 cut(s)	1551
MboI	GATC	4 cut(s)	909, 943, 1351, 1873
MboII	GAAGA	3 cut(s)	11, 14, 1290
MfeI	CAATTG	3 cut(s)	505, 959, 1133
MflI	RGATCY	2 cut(s)	909, 1351
MhlI	GDGCHC	2 cut(s)	859, 1885
MlyI	GAGTC	1 cut(s)	197
MmeI	TCCRAC	1 cut(s)	766
Mph1103I	ATGCAT	3 cut(s)	413, 813, 817
MroI	TCCGGA	1 cut(s)	988
MroXI	GAANNNNTTC	1 cut(s)	1749
MseI	TTAA	7 cut(s)	96, 270, 555, 861, 1140, 1455, 1898
MslI	CAYNNNNRTG	1 cut(s)	1497
MspA1I	CMGCKG	1 cut(s)	1104
MspI	CCGG	5 cut(s)	288, 401, 571, 989, 1572
MspR9I	CCNGG	7 cut(s)	191, 289, 571, 1072, 1290, 1481, 1572
MunI	CAATTG	3 cut(s)	505, 959, 1133
Mva1269I	GAATGC	3 cut(s)	1252, 1313, 1647
MvaI	CCWGG	4 cut(s)	191, 1072, 1290, 1481
MwoI	GCNNNNNNNGC	3 cut(s)	629, 1196, 1532
NciI	CCSGG	3 cut(s)	289, 571, 1572
NdeII	GATC	4 cut(s)	909, 943, 1351, 1873
NlaIII	CATG	8 cut(s)	815, 854, 983, 1194, 1300, 1763, 1790, 1868
NlaIV	GGNNCC	4 cut(s)	1002, 1882, 1883, 1884
NmuCI	GTSAC	1 cut(s)	311
NsiI	ATGCAT	3 cut(s)	413, 813, 817
NspI	RCATGY	4 cut(s)	815, 983, 1194, 1868
PaeI	GCATGC	1 cut(s)	815
PceI	AGGCCT	1 cut(s)	107
PciI	ACATGT	1 cut(s)	1864
PctI	GAATGC	3 cut(s)	1252, 1313, 1647
PdmI	GAANNNNTTC	1 cut(s)	1749
PfeI	GAWTC	5 cut(s)	16, 475, 583, 1211, 1484
PkrI	GCNGC	3 cut(s)	349, 1106, 1647
PleI	GAGTC	1 cut(s)	197
PpsI	GAGTC	1 cut(s)	197
PscI	ACATGT	1 cut(s)	1864
PshBI	ATTAAT	2 cut(s)	1455, 1898
PsiI	TTATAA	1 cut(s)	54
Psp1406I	AACGTT	1 cut(s)	1474
Psp6I	CCWGG	4 cut(s)	189, 1070, 1288, 1479
PspGI	CCWGG	4 cut(s)	189, 1070, 1288, 1479
PspN4I	GGNNCC	4 cut(s)	1002, 1882, 1883, 1884
PspOMI	GGGCCC	1 cut(s)	1881
PspPI	GGNCC	4 cut(s)	1016, 1079, 1881, 1882
PstNI	CAGNNNCTG	3 cut(s)	1355, 1673, 1753
PsuI	RGATCY	2 cut(s)	909, 1351
RsaI	GTAC	7 cut(s)	405, 688, 906, 1002, 1517, 1584, 1892
RsaNI	GTAC	7 cut(s)	404, 687, 905, 1001, 1516, 1583, 1891
RseI	CAYNNNNRTG	1 cut(s)	1497
SaqAI	TTAA	7 cut(s)	96, 270, 555, 861, 1140, 1455, 1898
SatI	GCNGC	3 cut(s)	348, 1105, 1646
Sau3AI	GATC	4 cut(s)	909, 943, 1351, 1873
Sau96I	GGNCC	4 cut(s)	1016, 1079, 1881, 1882
ScaI	AGTACT	1 cut(s)	1584
SchI	GAGTC	1 cut(s)	197
ScrFI	CCNGG	7 cut(s)	191, 289, 571, 1072, 1290, 1481, 1572
SduI	GDGCHC	2 cut(s)	859, 1885
SfaNI	GCATC	5 cut(s)	115, 398, 599, 730, 1302
SinI	GGWCC	1 cut(s)	1079
SmiMI	CAYNNNNRTG	1 cut(s)	1497
SmlI	CTYRAG	4 cut(s)	470, 522, 1823, 1918
SmoI	CTYRAG	4 cut(s)	470, 522, 1823, 1918
SphI	GCATGC	1 cut(s)	815
SseBI	AGGCCT	1 cut(s)	107
SsiI	CCGC	2 cut(s)	1104, 1549
SspMI	CTAG	2 cut(s)	1563, 1580
StuI	AGGCCT	1 cut(s)	107
StyD4I	CCNGG	7 cut(s)	189, 287, 569, 1070, 1288, 1479, 1570
StyI	CCWWGG	1 cut(s)	161
TaaI	ACNGT	7 cut(s)	65, 354, 427, 1174, 1599, 1687, 1749
TaiI	ACGT	1 cut(s)	1477
TaqI	TCGA	3 cut(s)	593, 1633, 1662
TaqII	GACCGA	1 cut(s)	1836
TatI	WGTACW	4 cut(s)	686, 904, 1582, 1890
TauI	GCSGC	1 cut(s)	1107
TfiI	GAWTC	5 cut(s)	16, 475, 583, 1211, 1484
Tru1I	TTAA	7 cut(s)	96, 270, 555, 861, 1140, 1455, 1898
Tru9I	TTAA	7 cut(s)	96, 270, 555, 861, 1140, 1455, 1898
TscAI	CASTG	4 cut(s)	70, 1089, 1502, 1604
TseFI	GTSAC	1 cut(s)	311
TseI	GCWGC	2 cut(s)	347, 1645
Tsp45I	GTSAC	1 cut(s)	311
TspDTI	ATGAA	5 cut(s)	30, 1082, 1510, 1754, 1827
TspRI	CASTG	4 cut(s)	70, 1089, 1502, 1604
VpaK11BI	GGWCC	1 cut(s)	1079
VspI	ATTAAT	2 cut(s)	1455, 1898
XapI	RAATTY	6 cut(s)	155, 515, 838, 1088, 1216, 1435
XceI	RCATGY	4 cut(s)	815, 983, 1194, 1868
XcmI	CCANNNNNNNNNTGG	2 cut(s)	927, 1632
XmiI	GTMKAC	1 cut(s)	7
XmnI	GAANNNNTTC	1 cut(s)	1749
XspI	CTAG	2 cut(s)	1563, 1580
ZrmI	AGTACT	1 cut(s)	1584
Zsp2I	ATGCAT	3 cut(s)	413, 813, 817

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.