MD04G1096700.v1.1
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013801
Physical Location & Seq
Forward (+)
17869972 .. 17873617
3646 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1096700.v1.1.491

Sequence Viewer

Length: 678 bp
ATGGAGATTGAGAGTGAAATGCAGCAGCAATCCAGGTTTAGGAGGATTTGTGTGTTTTGTGGGAGCAGCCAAGGGAAGAAGAGCAGTTACCAGGATGCTGCCATTGAGCTTGGCAAAGAACTGAATGCATGCCAAAAAAATGTTTCAAGGAACATTGATCTGGTGTATGGAGGGGGCAGCATAGGACTGATGGGTTTGGTTTCACAAGCTGTTCATGATGGTGGTCGCCATGTCATTGGAGTTATTCCCAAGACGCTCATGCCTCGAGAGTTAACTGGTGAGACTGTGGGGGAAGTGAAGGCAGTTGCAGGCATGCATCAAAGAAAGGCAGAGATGGCTAAGCACTCAGATGCTTTTATTGCCTTGCCCGGTGGTTACGGAACTCTGGAAGAGTTACTCGAAGTCATAACATGGGCTCAGCTTGGAATTCATGACAAGCCAGTGGGATTGCTGAATGTCGATGGATACTACAACTCCTTGCTATCGTTCATCGACAAGGCTGTGGAAGAGGGATTTGTTAGCCCGAGCGCCCGCCACATAATTATATCGGCGCCAACAGCCAAGGAGCTGGTGAAGAAACTGGAGGATTACGTCCCATGTCATGAAAGAGTTGCTCCAAAGTTGAATTGGGAGATAGAGCAGCTTGGCTACACACAAGAATATGACATCTCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

226

Amino Acids

24.79

Weight (kDa)

5.83

Isoelectric Point (pI)

51.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LDcluster4 PF18306 21 - 152 1.1e-11 SLOG cluster4 family
Lysine_decarbox PF03641 64 - 194 1.5e-49 Possible lysine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013801)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37210 AT2G37210 AT3G53450
fragaria_vesca FvH4_6g16020
malus_domestica MD04G1096700.v1.1 MD12G1117700.v1.1
prunus_persica Prupe.6G236000_v2.0.a1 Prupe.6G236000_v2.0.a1
pyrus_communis pycom04g09180 pycom12g11650
rosa_chinensis RchiOBHm_Chr3g0469741
rosa_laevigata RLG00000024325
rosa_multiflora Rmu_sc0010644.1_g000003
rosa_roxburghii Rroxscaffold_6G00411510
rosa_rugosa Rorug03G0103900
rosa_samantha Rh3AG153300
rosa_wichuraiana Rw3G014450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 550
AciI CCGC 1 cut(s) 532
AcsI RAATTY 1 cut(s) 426
AcyI GRCGYC 1 cut(s) 551
AfiI CCNNNNNNNGG 1 cut(s) 39
AgsI TTSAA 2 cut(s) 147, 625
AjnI CCWGG 2 cut(s) 32, 90
AluBI AGCT 5 cut(s) 109, 209, 421, 568, 643
AluI AGCT 5 cut(s) 109, 209, 421, 568, 643
Alw26I GTCTC 1 cut(s) 275
Ama87I CYCGRG 2 cut(s) 264, 523
ApeKI GCWGC 6 cut(s) 22, 25, 66, 98, 177, 640
ApoI RAATTY 1 cut(s) 426
AspLEI GCGC 2 cut(s) 530, 553
AsuC2I CCSGG 1 cut(s) 369
AsuHPI GGTGA 2 cut(s) 290, 583
AvaI CYCGRG 2 cut(s) 264, 523
BanI GGYRCC 1 cut(s) 550
BanII GRGCYC 1 cut(s) 418
BarI GAAGNNNNNNTAC 2 cut(s) 71, 103
BbvI GCAGC 6 cut(s) 34, 37, 78, 85, 189, 652
BccI CCATC 4 cut(s) 184, 212, 328, 455
BciT130I CCWGG 2 cut(s) 34, 92
BciVI GTATCC 1 cut(s) 458
BcnI CCSGG 1 cut(s) 369
BcoDI GTCTC 1 cut(s) 275
BfaI CTAG 1 cut(s) 672
BfoI RGCGCY 2 cut(s) 531, 554
BfuI GTATCC 1 cut(s) 458
BisI GCNGC 6 cut(s) 23, 26, 67, 99, 178, 641
BlpI GCTNAGC 2 cut(s) 339, 417
BlsI GCNGC 6 cut(s) 24, 27, 68, 100, 179, 642
Bme1390I CCNGG 3 cut(s) 34, 92, 369
BmeT110I CYCGRG 2 cut(s) 264, 523
BmiI GGNNCC 1 cut(s) 552
BmrFI CCNGG 3 cut(s) 34, 92, 369
BmsI GCATC 3 cut(s) 85, 325, 340
BpmI CTGGAG 1 cut(s) 602
Bpu1102I GCTNAGC 2 cut(s) 339, 417
BpuMI CCSGG 1 cut(s) 369
BsaHI GRCGYC 1 cut(s) 551
BsaJI CCNNGG 2 cut(s) 70, 561
BsaXI ACNNNNNCTCC 4 cut(s) 34, 64, 458, 488
Bsc4I CCNNNNNNNGG 1 cut(s) 39
Bse1I ACTGG 3 cut(s) 280, 440, 585
BseBI CCWGG 2 cut(s) 34, 92
BseDI CCNNGG 2 cut(s) 70, 561
BseGI GGATG 1 cut(s) 100
BseLI CCNNNNNNNGG 1 cut(s) 39
BseMII CTCAG 2 cut(s) 360, 431
BseNI ACTGG 3 cut(s) 280, 440, 585
BseXI GCAGC 6 cut(s) 34, 37, 78, 85, 189, 652
BshNI GGYRCC 1 cut(s) 550
BsiHKCI CYCGRG 2 cut(s) 264, 523
BsiSI CCGG 1 cut(s) 369
BslFI GGGAC 1 cut(s) 578
BslI CCNNNNNNNGG 1 cut(s) 39
BsmAI GTCTC 1 cut(s) 275
BsmFI GGGAC 1 cut(s) 578
BsmI GAATGC 1 cut(s) 130
BsoBI CYCGRG 2 cut(s) 264, 523
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 1 cut(s) 157
Bsp1720I GCTNAGC 2 cut(s) 339, 417
BspACI CCGC 1 cut(s) 532
BspCNI CTCAG 2 cut(s) 359, 430
BspHI TCATGA 3 cut(s) 214, 430, 601
BspLI GGNNCC 1 cut(s) 552
BspQI GCTCTTC 1 cut(s) 74
BspT107I GGYRCC 1 cut(s) 550
BsrI ACTGG 3 cut(s) 280, 440, 585
BssECI CCNNGG 2 cut(s) 70, 561
BssMI GATC 1 cut(s) 157
BssNI GRCGYC 1 cut(s) 551
BssT1I CCWWGG 2 cut(s) 70, 561
Bst2UI CCWGG 2 cut(s) 34, 92
Bst4CI ACNGT 1 cut(s) 286
Bst6I CTCTTC 3 cut(s) 74, 384, 501
BstACI GRCGYC 1 cut(s) 551
BstC8I GCNNGC 4 cut(s) 130, 310, 314, 532
BstDEI CTNAG 3 cut(s) 339, 346, 417
BstF5I GGATG 1 cut(s) 100
BstH2I RGCGCY 2 cut(s) 531, 554
BstHHI GCGC 2 cut(s) 530, 553
BstKTI GATC 1 cut(s) 160
BstMAI GTCTC 1 cut(s) 275
BstMBI GATC 1 cut(s) 157
BstMWI GCNNNNNNNGC 3 cut(s) 335, 359, 557
BstNI CCWGG 2 cut(s) 34, 92
BstNSI RCATGY 2 cut(s) 132, 316
BstSCI CCNGG 3 cut(s) 32, 90, 367
BstV1I GCAGC 6 cut(s) 34, 37, 78, 85, 189, 652
BstXI CCANNNNNNTGG 2 cut(s) 236, 568
BsuI GTATCC 1 cut(s) 458
BtsCI GGATG 1 cut(s) 100
BtsIMutI CAGTG 1 cut(s) 447
Cac8I GCNNGC 4 cut(s) 130, 310, 314, 532
CciI TCATGA 3 cut(s) 214, 430, 601
CfoI GCGC 2 cut(s) 530, 553
CseI GACGC 1 cut(s) 262
CviAII CATG 9 cut(s) 129, 215, 230, 259, 313, 411, 431, 597, 602
DdeI CTNAG 3 cut(s) 339, 346, 417
DinI GGCGCC 1 cut(s) 552
DpnI GATC 1 cut(s) 159
DpnII GATC 1 cut(s) 157
Eam1104I CTCTTC 3 cut(s) 74, 384, 501
EarI CTCTTC 3 cut(s) 74, 384, 501
Eco130I CCWWGG 2 cut(s) 70, 561
Eco24I GRGCYC 1 cut(s) 418
Eco88I CYCGRG 2 cut(s) 264, 523
EcoRI GAATTC 1 cut(s) 426
EcoRII CCWGG 2 cut(s) 32, 90
EcoT14I CCWWGG 2 cut(s) 70, 561
EcoT22I ATGCAT 2 cut(s) 130, 318
EcoT38I GRGCYC 1 cut(s) 418
EgeI GGCGCC 1 cut(s) 552
EheI GGCGCC 1 cut(s) 552
ErhI CCWWGG 2 cut(s) 70, 561
FaeI CATG 9 cut(s) 132, 218, 233, 262, 316, 414, 434, 600, 605
FaqI GGGAC 1 cut(s) 578
FatI CATG 9 cut(s) 128, 214, 229, 258, 312, 410, 430, 596, 601
FauI CCCGC 1 cut(s) 539
Fnu4HI GCNGC 6 cut(s) 23, 26, 67, 99, 178, 641
FokI GGATG 1 cut(s) 107
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 6 cut(s) 23, 26, 67, 99, 178, 641
FspBI CTAG 1 cut(s) 672
GlaI GCGC 2 cut(s) 529, 552
GluI GCNGC 6 cut(s) 23, 26, 67, 99, 178, 641
GsuI CTGGAG 1 cut(s) 602
HaeII RGCGCY 2 cut(s) 531, 554
HapII CCGG 1 cut(s) 369
HgaI GACGC 1 cut(s) 262
HhaI GCGC 2 cut(s) 530, 553
Hin1I GRCGYC 1 cut(s) 551
Hin1II CATG 9 cut(s) 132, 218, 233, 262, 316, 414, 434, 600, 605
Hin6I GCGC 2 cut(s) 528, 551
HinP1I GCGC 2 cut(s) 528, 551
HincII GTYRAC 1 cut(s) 273
HindII GTYRAC 1 cut(s) 273
HpaI GTTAAC 1 cut(s) 273
HpaII CCGG 1 cut(s) 369
HphI GGTGA 2 cut(s) 290, 583
Hpy166II GTNNAC 1 cut(s) 273
Hpy188I TCNGA 1 cut(s) 349
Hpy188III TCNNGA 6 cut(s) 215, 266, 386, 431, 602, 672
Hpy8I GTNNAC 1 cut(s) 273
HpyAV CCTTC 1 cut(s) 292
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 1 cut(s) 591
HpyCH4V TGCA 4 cut(s) 22, 128, 308, 316
HpyF10VI GCNNNNNNNGC 3 cut(s) 335, 359, 557
HpyF3I CTNAG 3 cut(s) 339, 346, 417
HpySE526I ACGT 1 cut(s) 591
Hsp92I GRCGYC 1 cut(s) 551
Hsp92II CATG 9 cut(s) 132, 218, 233, 262, 316, 414, 434, 600, 605
HspAI GCGC 2 cut(s) 528, 551
KasI GGCGCC 1 cut(s) 550
KspAI GTTAAC 1 cut(s) 273
Kzo9I GATC 1 cut(s) 157
LguI GCTCTTC 1 cut(s) 74
LmnI GCTCC 3 cut(s) 63, 565, 619
Lsp1109I GCAGC 6 cut(s) 34, 37, 78, 85, 189, 652
LweI GCATC 3 cut(s) 85, 325, 340
MaeI CTAG 1 cut(s) 672
MaeII ACGT 1 cut(s) 591
MaeIII GTNAC 3 cut(s) 86, 374, 393
MalI GATC 1 cut(s) 159
MboI GATC 1 cut(s) 157
MboII GAAGA 5 cut(s) 88, 91, 401, 518, 586
MhlI GDGCHC 1 cut(s) 418
MluCI AATT 3 cut(s) 426, 540, 625
Mly113I GGCGCC 1 cut(s) 551
MnlI CCTC 5 cut(s) 36, 164, 273, 502, 577
Mph1103I ATGCAT 2 cut(s) 130, 318
MseI TTAA 1 cut(s) 272
MslI CAYNNNNRTG 2 cut(s) 219, 348
MspI CCGG 1 cut(s) 369
MspR9I CCNGG 3 cut(s) 34, 92, 369
Mva1269I GAATGC 1 cut(s) 130
MvaI CCWGG 2 cut(s) 34, 92
MwoI GCNNNNNNNGC 3 cut(s) 335, 359, 557
NarI GGCGCC 1 cut(s) 551
NciI CCSGG 1 cut(s) 369
NdeII GATC 1 cut(s) 157
NlaIII CATG 9 cut(s) 132, 218, 233, 262, 316, 414, 434, 600, 605
NlaIV GGNNCC 1 cut(s) 552
NsiI ATGCAT 2 cut(s) 130, 318
NspI RCATGY 2 cut(s) 132, 316
PaeI GCATGC 2 cut(s) 132, 316
PaeR7I CTCGAG 1 cut(s) 264
PagI TCATGA 3 cut(s) 214, 430, 601
PciSI GCTCTTC 1 cut(s) 74
PctI GAATGC 1 cut(s) 130
PkrI GCNGC 6 cut(s) 24, 27, 68, 100, 179, 642
PluTI GGCGCC 1 cut(s) 554
Psp6I CCWGG 2 cut(s) 32, 90
PspGI CCWGG 2 cut(s) 32, 90
PspN4I GGNNCC 1 cut(s) 552
RseI CAYNNNNRTG 2 cut(s) 219, 348
SapI GCTCTTC 1 cut(s) 74
SaqAI TTAA 1 cut(s) 272
SatI GCNGC 6 cut(s) 23, 26, 67, 99, 178, 641
Sau3AI GATC 1 cut(s) 157
ScrFI CCNGG 3 cut(s) 34, 92, 369
SduI GDGCHC 1 cut(s) 418
SetI ASST 7 cut(s) 38, 111, 211, 423, 570, 594, 645
SfaNI GCATC 3 cut(s) 85, 325, 340
SfoI GGCGCC 1 cut(s) 552
Sfr274I CTCGAG 1 cut(s) 264
SlaI CTCGAG 1 cut(s) 264
SmiMI CAYNNNNRTG 2 cut(s) 219, 348
SmlI CTYRAG 1 cut(s) 264
SmoI CTYRAG 1 cut(s) 264
SphI GCATGC 2 cut(s) 132, 316
Sse9I AATT 3 cut(s) 426, 540, 625
SsiI CCGC 1 cut(s) 532
SspDI GGCGCC 1 cut(s) 550
SspMI CTAG 1 cut(s) 672
StyD4I CCNGG 3 cut(s) 32, 90, 367
StyI CCWWGG 2 cut(s) 70, 561
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 1 cut(s) 594
TaqI TCGA 4 cut(s) 265, 399, 459, 492
TasI AATT 3 cut(s) 426, 540, 625
Tru1I TTAA 1 cut(s) 272
Tru9I TTAA 1 cut(s) 272
TscAI CASTG 1 cut(s) 447
TseI GCWGC 6 cut(s) 22, 25, 66, 98, 177, 640
TspDTI ATGAA 4 cut(s) 203, 419, 478, 618
TspGWI ACGGA 1 cut(s) 393
TspRI CASTG 1 cut(s) 447
XapI RAATTY 1 cut(s) 426
XbaI TCTAGA 1 cut(s) 671
XceI RCATGY 2 cut(s) 132, 316
XcmI CCANNNNNNNNNTGG 1 cut(s) 624
XhoI CTCGAG 1 cut(s) 264
XspI CTAG 1 cut(s) 672
Zsp2I ATGCAT 2 cut(s) 130, 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.