pycom12g11650
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0013801
Physical Location & Seq
Forward (+)
14008903 .. 14012438
3536 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g11650.1

Sequence Viewer

Length: 660 bp
ATGGAGATTGAGAGTGAAATGAAGCAGCAATCCAGGTTTAGGAGGATTTGTGTGTTTTGTGGGAGCAGCCAAGGGAAGAAGAGCAGCTATCACGATGCTGCCATTGAACTTGGCAAGGAACTGGTTTCAAGGAACATTGATCTGGTGTATGGAGGGGGAAGCATAGGACTGATGGGTTTGGTCTCACAAGCAGTTCATGATGGTGGTCGCCATGTCATTGGAGTTATTCCCAAGACGCTCATGCCTCGAGAGTTAACTGGTGAGACTGTGGGGGAAGTGAAGGCAGTTGCAGGCATGCATCAAAGAAAGGCAGAGATGGCTAAGCACTCAGATGCTTTTATTGCCTTACCCGGTGGTTATGGAACTCTGGAAGAGTTGCTTGAAGTTATAACCTGGGCTCAGCTTGGAATTCATGACAAGCCTGTGGGATTGCTGAATGTTGATGGATACTACAATTCCTTACTATCGTTCATCGATAAGGCCGTGGAAGAGGGATTTGTTAGCCCGAGCGCACGCCACATAATTATATCAGCACCAACAGCCAAGGAGCTGGTGAAGAAGCTGGAGGATTATGTCCCATGTCATGAAAGAGTTGCTTCAAAGTTGAATTGGGAGATGGAGCAGCTTGGCTACACACAAGAATGCGACATCTCAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

220

Amino Acids

24.09

Weight (kDa)

5.92

Isoelectric Point (pI)

48.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LDcluster4 PF18306 21 - 146 8.2e-15 SLOG cluster4 family
Lysine_decarbox PF03641 58 - 188 1.4e-49 Possible lysine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013801)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37210 AT2G37210 AT3G53450
fragaria_vesca FvH4_6g16020
malus_domestica MD04G1096700.v1.1 MD12G1117700.v1.1
prunus_persica Prupe.6G236000_v2.0.a1 Prupe.6G236000_v2.0.a1
pyrus_communis pycom04g09180 pycom12g11650
rosa_chinensis RchiOBHm_Chr3g0469741
rosa_laevigata RLG00000024325
rosa_multiflora Rmu_sc0010644.1_g000003
rosa_roxburghii Rroxscaffold_6G00411510
rosa_rugosa Rorug03G0103900
rosa_samantha Rh3AG153300
rosa_wichuraiana Rw3G014450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 389
AcsI RAATTY 1 cut(s) 408
AfiI CCNNNNNNNGG 1 cut(s) 39
AgsI TTSAA 5 cut(s) 107, 129, 383, 600, 607
AjnI CCWGG 2 cut(s) 32, 392
AluBI AGCT 5 cut(s) 87, 403, 550, 562, 625
AluI AGCT 5 cut(s) 87, 403, 550, 562, 625
Alw26I GTCTC 2 cut(s) 187, 257
Ama87I CYCGRG 2 cut(s) 246, 505
AoxI GGCC 1 cut(s) 480
ApeKI GCWGC 5 cut(s) 25, 66, 84, 98, 622
ApoI RAATTY 1 cut(s) 408
AspLEI GCGC 1 cut(s) 512
AsuC2I CCSGG 1 cut(s) 351
AsuHPI GGTGA 2 cut(s) 272, 565
AvaI CYCGRG 2 cut(s) 246, 505
BanII GRGCYC 1 cut(s) 400
BbvI GCAGC 5 cut(s) 37, 78, 85, 96, 634
BccI CCATC 5 cut(s) 166, 194, 310, 437, 610
BceAI ACGGC 1 cut(s) 467
BciT130I CCWGG 2 cut(s) 34, 394
BciVI GTATCC 1 cut(s) 440
BcnI CCSGG 1 cut(s) 351
BcoDI GTCTC 2 cut(s) 187, 257
BfuI GTATCC 1 cut(s) 440
BisI GCNGC 5 cut(s) 26, 67, 85, 99, 623
BlpI GCTNAGC 2 cut(s) 321, 399
BlsI GCNGC 5 cut(s) 27, 68, 86, 100, 624
Bme1390I CCNGG 3 cut(s) 34, 351, 394
BmeT110I CYCGRG 2 cut(s) 246, 505
BmrFI CCNGG 3 cut(s) 34, 351, 394
BmsI GCATC 3 cut(s) 85, 307, 322
BpmI CTGGAG 1 cut(s) 584
Bpu1102I GCTNAGC 2 cut(s) 321, 399
BpuEI CTTGAG 1 cut(s) 637
BpuMI CCSGG 1 cut(s) 351
Bsa29I ATCGAT 1 cut(s) 474
BsaI GGTCTC 1 cut(s) 187
BsaJI CCNNGG 4 cut(s) 70, 393, 483, 543
BsaXI ACNNNNNCTCC 2 cut(s) 34, 64
Bsc4I CCNNNNNNNGG 1 cut(s) 39
Bse1I ACTGG 2 cut(s) 126, 262
BseBI CCWGG 2 cut(s) 34, 394
BseCI ATCGAT 1 cut(s) 474
BseDI CCNNGG 4 cut(s) 70, 393, 483, 543
BseLI CCNNNNNNNGG 1 cut(s) 39
BseMII CTCAG 2 cut(s) 342, 413
BseNI ACTGG 2 cut(s) 126, 262
BseXI GCAGC 5 cut(s) 37, 78, 85, 96, 634
BshFI GGCC 1 cut(s) 482
BshVI ATCGAT 1 cut(s) 474
BsiHKCI CYCGRG 2 cut(s) 246, 505
BsiSI CCGG 1 cut(s) 351
BslFI GGGAC 1 cut(s) 560
BslI CCNNNNNNNGG 1 cut(s) 39
BsmAI GTCTC 2 cut(s) 187, 257
BsmFI GGGAC 1 cut(s) 560
BsmI GAATGC 1 cut(s) 647
BsnI GGCC 1 cut(s) 482
Bso31I GGTCTC 1 cut(s) 187
BsoBI CYCGRG 2 cut(s) 246, 505
Bsp1286I GDGCHC 1 cut(s) 400
Bsp143I GATC 1 cut(s) 139
Bsp1720I GCTNAGC 2 cut(s) 321, 399
BspANI GGCC 1 cut(s) 482
BspCNI CTCAG 2 cut(s) 341, 412
BspDI ATCGAT 1 cut(s) 474
BspHI TCATGA 3 cut(s) 196, 412, 583
BspQI GCTCTTC 1 cut(s) 74
BspTNI GGTCTC 1 cut(s) 187
BsrI ACTGG 2 cut(s) 126, 262
BssECI CCNNGG 4 cut(s) 70, 393, 483, 543
BssMI GATC 1 cut(s) 139
BssT1I CCWWGG 2 cut(s) 70, 543
Bst2UI CCWGG 2 cut(s) 34, 394
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 3 cut(s) 74, 366, 483
BstC8I GCNNGC 3 cut(s) 292, 296, 514
BstDEI CTNAG 3 cut(s) 321, 328, 399
BstDSI CCRYGG 1 cut(s) 483
BstHHI GCGC 1 cut(s) 512
BstKTI GATC 1 cut(s) 142
BstMAI GTCTC 2 cut(s) 187, 257
BstMBI GATC 1 cut(s) 139
BstMWI GCNNNNNNNGC 3 cut(s) 317, 341, 539
BstNI CCWGG 2 cut(s) 34, 394
BstNSI RCATGY 1 cut(s) 298
BstSCI CCNGG 3 cut(s) 32, 349, 392
BstV1I GCAGC 5 cut(s) 37, 78, 85, 96, 634
BstXI CCANNNNNNTGG 2 cut(s) 218, 550
Bsu15I ATCGAT 1 cut(s) 474
BsuI GTATCC 1 cut(s) 440
BsuRI GGCC 1 cut(s) 482
BsuTUI ATCGAT 1 cut(s) 474
BtgI CCRYGG 1 cut(s) 483
Cac8I GCNNGC 3 cut(s) 292, 296, 514
CciI TCATGA 3 cut(s) 196, 412, 583
CfoI GCGC 1 cut(s) 512
ClaI ATCGAT 1 cut(s) 474
CseI GACGC 1 cut(s) 244
CviAII CATG 7 cut(s) 197, 212, 241, 295, 413, 579, 584
DdeI CTNAG 3 cut(s) 321, 328, 399
DpnI GATC 1 cut(s) 141
DpnII GATC 1 cut(s) 139
Eam1104I CTCTTC 3 cut(s) 74, 366, 483
EarI CTCTTC 3 cut(s) 74, 366, 483
Eco130I CCWWGG 2 cut(s) 70, 543
Eco24I GRGCYC 1 cut(s) 400
Eco31I GGTCTC 1 cut(s) 187
Eco88I CYCGRG 2 cut(s) 246, 505
EcoRI GAATTC 1 cut(s) 408
EcoRII CCWGG 2 cut(s) 32, 392
EcoT14I CCWWGG 2 cut(s) 70, 543
EcoT22I ATGCAT 1 cut(s) 300
EcoT38I GRGCYC 1 cut(s) 400
ErhI CCWWGG 2 cut(s) 70, 543
FaeI CATG 7 cut(s) 200, 215, 244, 298, 416, 582, 587
FalI AAGNNNNNCTT 4 cut(s) 363, 395, 580, 612
FaqI GGGAC 1 cut(s) 560
FatI CATG 7 cut(s) 196, 211, 240, 294, 412, 578, 583
Fnu4HI GCNGC 5 cut(s) 26, 67, 85, 99, 623
FriOI GRGCYC 1 cut(s) 400
Fsp4HI GCNGC 5 cut(s) 26, 67, 85, 99, 623
GlaI GCGC 1 cut(s) 511
GluI GCNGC 5 cut(s) 26, 67, 85, 99, 623
GsuI CTGGAG 1 cut(s) 584
HaeIII GGCC 1 cut(s) 482
HapII CCGG 1 cut(s) 351
HgaI GACGC 1 cut(s) 244
HhaI GCGC 1 cut(s) 512
Hin1II CATG 7 cut(s) 200, 215, 244, 298, 416, 582, 587
Hin6I GCGC 1 cut(s) 510
HinP1I GCGC 1 cut(s) 510
HincII GTYRAC 1 cut(s) 255
HindII GTYRAC 1 cut(s) 255
HpaI GTTAAC 1 cut(s) 255
HpaII CCGG 1 cut(s) 351
HphI GGTGA 2 cut(s) 272, 565
Hpy166II GTNNAC 1 cut(s) 255
Hpy188I TCNGA 1 cut(s) 331
Hpy188III TCNNGA 6 cut(s) 92, 197, 248, 368, 413, 584
Hpy8I GTNNAC 1 cut(s) 255
HpyAV CCTTC 1 cut(s) 274
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4V TGCA 2 cut(s) 290, 298
HpyF10VI GCNNNNNNNGC 3 cut(s) 317, 341, 539
HpyF3I CTNAG 3 cut(s) 321, 328, 399
Hsp92II CATG 7 cut(s) 200, 215, 244, 298, 416, 582, 587
HspAI GCGC 1 cut(s) 510
KspAI GTTAAC 1 cut(s) 255
Kzo9I GATC 1 cut(s) 139
LguI GCTCTTC 1 cut(s) 74
LmnI GCTCC 3 cut(s) 63, 547, 619
Lsp1109I GCAGC 5 cut(s) 37, 78, 85, 96, 634
LweI GCATC 3 cut(s) 85, 307, 322
MalI GATC 1 cut(s) 141
MboI GATC 1 cut(s) 139
MboII GAAGA 5 cut(s) 88, 91, 383, 500, 568
MhlI GDGCHC 1 cut(s) 400
MluCI AATT 4 cut(s) 408, 454, 522, 607
MnlI CCTC 5 cut(s) 36, 146, 255, 484, 559
Mph1103I ATGCAT 1 cut(s) 300
MseI TTAA 1 cut(s) 254
MslI CAYNNNNRTG 3 cut(s) 201, 330, 640
MspI CCGG 1 cut(s) 351
MspR9I CCNGG 3 cut(s) 34, 351, 394
Mva1269I GAATGC 1 cut(s) 647
MvaI CCWGG 2 cut(s) 34, 394
MwoI GCNNNNNNNGC 3 cut(s) 317, 341, 539
NciI CCSGG 1 cut(s) 351
NdeII GATC 1 cut(s) 139
NlaIII CATG 7 cut(s) 200, 215, 244, 298, 416, 582, 587
NsiI ATGCAT 1 cut(s) 300
NspI RCATGY 1 cut(s) 298
PaeI GCATGC 1 cut(s) 298
PaeR7I CTCGAG 1 cut(s) 246
PagI TCATGA 3 cut(s) 196, 412, 583
PciSI GCTCTTC 1 cut(s) 74
PcsI WCGNNNNNNNCGW 1 cut(s) 480
PctI GAATGC 1 cut(s) 647
PkrI GCNGC 5 cut(s) 27, 68, 86, 100, 624
PsiI TTATAA 1 cut(s) 389
Psp6I CCWGG 2 cut(s) 32, 392
PspGI CCWGG 2 cut(s) 32, 392
RseI CAYNNNNRTG 3 cut(s) 201, 330, 640
SapI GCTCTTC 1 cut(s) 74
SaqAI TTAA 1 cut(s) 254
SatI GCNGC 5 cut(s) 26, 67, 85, 99, 623
Sau3AI GATC 1 cut(s) 139
ScrFI CCNGG 3 cut(s) 34, 351, 394
SduI GDGCHC 1 cut(s) 400
SetI ASST 8 cut(s) 38, 89, 395, 405, 552, 564, 627, 659
SfaNI GCATC 3 cut(s) 85, 307, 322
Sfr274I CTCGAG 1 cut(s) 246
SlaI CTCGAG 1 cut(s) 246
SmiMI CAYNNNNRTG 3 cut(s) 201, 330, 640
SmlI CTYRAG 2 cut(s) 246, 652
SmoI CTYRAG 2 cut(s) 246, 652
SphI GCATGC 1 cut(s) 298
Sse9I AATT 4 cut(s) 408, 454, 522, 607
StyD4I CCNGG 3 cut(s) 32, 349, 392
StyI CCWWGG 2 cut(s) 70, 543
TaaI ACNGT 1 cut(s) 268
TaqI TCGA 2 cut(s) 247, 474
TasI AATT 4 cut(s) 408, 454, 522, 607
Tru1I TTAA 1 cut(s) 254
Tru9I TTAA 1 cut(s) 254
TseI GCWGC 5 cut(s) 25, 66, 84, 98, 622
TspDTI ATGAA 5 cut(s) 35, 185, 401, 460, 600
XapI RAATTY 1 cut(s) 408
XceI RCATGY 1 cut(s) 298
XhoI CTCGAG 1 cut(s) 246
Zsp2I ATGCAT 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.