MD04G1116500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0012391
Physical Location & Seq
Forward (+)
20159601 .. 20160077
477 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1116500.v1.1.491

Sequence Viewer

Length: 477 bp
ATGACTTCAGTTTGCATATCAAACTGCTTCAACGACGCACGTGACCCCCGCGTGCCGATTCGGGCCACCTACGTAAACCTCTACAAGTGGCCCGAGTCCGACGCAGAGTTTGTGCGCTCGGTGAGCTCCAACGGGCGGCGAACGGATCCTACACTGCGACACGGGCACCCAAGGGTGGTGGACAGCATATCATGCAGGCAAATGTATCTCAGGAGCTATAAGTTTTCAAGGAAAGAGAGCGTGCCGGAGAAAACCCAGAAGTGCTTTGGAAGAGTGAAGGAGAAGATGGCAGGAAATAACAACAATAATGACGGGAGTAAGGGTAAGAAGGGGAAGGGAAAAGTGGGGAGGAAAAAGAATAGTAAGTGTTTGGTTCTCAGGAAAGTGAAGGAATTTTCATCTGCTGCTCTCTTTAGAATATTTCAGAGGTTGCTGTCTTGCTCTGCTAGTGTAGATGTTGTGAGTCATGATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.92

Weight (kDa)

10.07

Isoelectric Point (pI)

35.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012391)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02640
fragaria_vesca FvH4_6g14750
malus_domestica MD04G1116500.v1.1 MD12G1132300.v1.1
prunus_persica Prupe.4G261600_v2.0.a1 Prupe.4G261600_v2.0.a1 Prupe.6G246400_v2.0.a1
pyrus_communis pycom12g12820
rosa_chinensis RchiOBHm_Chr3g0467961
rosa_laevigata RLG00000024463
rosa_multiflora Rmu_sc0006017.1_g000003 Rmu_sc0034877.1_g000002
rosa_roxburghii Rroxscaffold_6G00413250
rosa_rugosa Rorug03G0090700
rosa_samantha Rh3AG139400 Rh3BG160900 Rh3CG160800 Rh3DG161400
rosa_wichuraiana Rw3G013190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 165
AccII CGCG 1 cut(s) 51
AciI CCGC 2 cut(s) 49, 136
AclWI GGATC 2 cut(s) 140, 153
AcsI RAATTY 1 cut(s) 392
AcvI CACGTG 1 cut(s) 41
AfiI CCNNNNNNNGG 2 cut(s) 135, 175
AgsI TTSAA 2 cut(s) 31, 228
AluBI AGCT 2 cut(s) 126, 216
AluI AGCT 2 cut(s) 126, 216
Alw21I GWGCWC 1 cut(s) 128
AlwI GGATC 2 cut(s) 140, 153
Ama87I CYCGRG 1 cut(s) 92
AoxI GGCC 2 cut(s) 63, 89
ApeKI GCWGC 1 cut(s) 404
ApoI RAATTY 1 cut(s) 392
ArsI GACNNNNNNTTYG 2 cut(s) 92, 124
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 2 cut(s) 63, 90
AsuHPI GGTGA 1 cut(s) 133
AvaI CYCGRG 1 cut(s) 92
BaeGI GKGCMC 1 cut(s) 168
BamHI GGATCC 1 cut(s) 145
BanI GGYRCC 1 cut(s) 165
BanII GRGCYC 1 cut(s) 128
BbrPI CACGTG 1 cut(s) 41
Bbv12I GWGCWC 1 cut(s) 128
BbvI GCAGC 1 cut(s) 391
BccI CCATC 1 cut(s) 280
BfaI CTAG 1 cut(s) 447
BisI GCNGC 2 cut(s) 137, 405
BlsI GCNGC 2 cut(s) 138, 406
BmeT110I CYCGRG 1 cut(s) 92
BmgT120I GGNCC 2 cut(s) 63, 90
BmiI GGNNCC 2 cut(s) 147, 167
BsaAI YACGTR 2 cut(s) 41, 73
BsaJI CCNNGG 1 cut(s) 170
BsaXI ACNNNNNCTCC 2 cut(s) 307, 337
Bsc4I CCNNNNNNNGG 2 cut(s) 135, 175
BseDI CCNNGG 1 cut(s) 170
BseLI CCNNNNNNNGG 2 cut(s) 135, 175
BseMII CTCAG 2 cut(s) 223, 391
BseSI GKGCMC 1 cut(s) 168
BseXI GCAGC 1 cut(s) 391
Bsh1236I CGCG 1 cut(s) 51
BshFI GGCC 2 cut(s) 65, 91
BshNI GGYRCC 1 cut(s) 165
BsiHKAI GWGCWC 1 cut(s) 128
BsiHKCI CYCGRG 1 cut(s) 92
BsiSI CCGG 1 cut(s) 245
BslI CCNNNNNNNGG 2 cut(s) 135, 175
BsnI GGCC 2 cut(s) 65, 91
BsoBI CYCGRG 1 cut(s) 92
Bsp1286I GDGCHC 2 cut(s) 128, 168
Bsp143I GATC 1 cut(s) 145
BspACI CCGC 2 cut(s) 49, 136
BspANI GGCC 2 cut(s) 65, 91
BspCNI CTCAG 2 cut(s) 222, 390
BspFNI CGCG 1 cut(s) 51
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 2 cut(s) 147, 167
BspPI GGATC 2 cut(s) 140, 153
BspT107I GGYRCC 1 cut(s) 165
BssECI CCNNGG 1 cut(s) 170
BssMI GATC 1 cut(s) 145
BssT1I CCWWGG 1 cut(s) 170
Bst6I CTCTTC 1 cut(s) 265
BstAPI GCANNNNNTGC 1 cut(s) 192
BstBAI YACGTR 2 cut(s) 41, 73
BstC8I GCNNGC 3 cut(s) 53, 197, 242
BstDEI CTNAG 2 cut(s) 209, 377
BstFNI CGCG 1 cut(s) 51
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 1 cut(s) 148
BstMBI GATC 1 cut(s) 145
BstMWI GCNNNNNNNGC 3 cut(s) 123, 163, 192
BstSLI GKGCMC 1 cut(s) 168
BstSNI TACGTA 1 cut(s) 73
BstUI CGCG 1 cut(s) 51
BstV1I GCAGC 1 cut(s) 391
BstX2I RGATCY 1 cut(s) 145
BstYI RGATCY 1 cut(s) 145
BsuRI GGCC 2 cut(s) 65, 91
BtsI GCAGTG 1 cut(s) 152
BtsIMutI CAGTG 1 cut(s) 152
Cac8I GCNNGC 3 cut(s) 53, 197, 242
CciI TCATGA 1 cut(s) 466
CfoI GCGC 1 cut(s) 117
Cfr13I GGNCC 2 cut(s) 63, 90
CseI GACGC 2 cut(s) 44, 110
CspCI CAANNNNNGTGG 2 cut(s) 159, 194
CviAII CATG 2 cut(s) 192, 467
CviJI RGCY 4 cut(s) 65, 91, 126, 216
CviKI_1 RGCY 4 cut(s) 65, 91, 126, 216
DdeI CTNAG 2 cut(s) 209, 377
DpnI GATC 1 cut(s) 147
DpnII GATC 1 cut(s) 145
Eam1104I CTCTTC 1 cut(s) 265
EarI CTCTTC 1 cut(s) 265
Ecl136II GAGCTC 1 cut(s) 126
Eco105I TACGTA 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 170
Eco24I GRGCYC 1 cut(s) 128
Eco53kI GAGCTC 1 cut(s) 126
Eco72I CACGTG 1 cut(s) 41
Eco88I CYCGRG 1 cut(s) 92
EcoICRI GAGCTC 1 cut(s) 126
EcoT14I CCWWGG 1 cut(s) 170
EcoT38I GRGCYC 1 cut(s) 128
ErhI CCWWGG 1 cut(s) 170
FaeI CATG 2 cut(s) 195, 470
FaiI YATR 5 cut(s) 17, 188, 193, 219, 468
FatI CATG 2 cut(s) 191, 466
FauI CCCGC 1 cut(s) 56
Fnu4HI GCNGC 2 cut(s) 137, 405
FriOI GRGCYC 1 cut(s) 128
Fsp4HI GCNGC 2 cut(s) 137, 405
FspBI CTAG 1 cut(s) 447
GlaI GCGC 1 cut(s) 116
GluI GCNGC 2 cut(s) 137, 405
HaeIII GGCC 2 cut(s) 65, 91
HapII CCGG 1 cut(s) 245
HgaI GACGC 2 cut(s) 44, 110
HhaI GCGC 1 cut(s) 117
Hin1II CATG 2 cut(s) 195, 470
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HinfI GANTC 3 cut(s) 58, 95, 463
HpaII CCGG 1 cut(s) 245
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 2 cut(s) 76, 181
Hpy188I TCNGA 2 cut(s) 100, 426
Hpy188III TCNNGA 3 cut(s) 211, 379, 467
Hpy8I GTNNAC 2 cut(s) 76, 181
Hpy99I CGWCG 2 cut(s) 38, 104
HpyAV CCTTC 4 cut(s) 271, 322, 328, 382
HpyCH4IV ACGT 2 cut(s) 40, 72
HpyCH4V TGCA 2 cut(s) 15, 195
HpyF10VI GCNNNNNNNGC 3 cut(s) 123, 163, 192
HpyF3I CTNAG 2 cut(s) 209, 377
HpySE526I ACGT 2 cut(s) 40, 72
Hsp92II CATG 2 cut(s) 195, 470
HspAI GCGC 1 cut(s) 115
Kzo9I GATC 1 cut(s) 145
LmnI GCTCC 2 cut(s) 131, 213
LpnPI CCDG 6 cut(s) 181, 196, 258, 269, 276, 364
Lsp1109I GCAGC 1 cut(s) 391
MaeI CTAG 1 cut(s) 447
MaeII ACGT 2 cut(s) 40, 72
MaeIII GTNAC 1 cut(s) 41
MalI GATC 1 cut(s) 147
MboI GATC 1 cut(s) 145
MboII GAAGA 2 cut(s) 282, 295
MflI RGATCY 1 cut(s) 145
MhlI GDGCHC 2 cut(s) 128, 168
MluCI AATT 1 cut(s) 392
MlyI GAGTC 2 cut(s) 104, 472
MmeI TCCRAC 2 cut(s) 123, 153
MnlI CCTC 3 cut(s) 89, 342, 420
MspI CCGG 1 cut(s) 245
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 3 cut(s) 123, 163, 192
NdeII GATC 1 cut(s) 145
NlaIII CATG 2 cut(s) 195, 470
NlaIV GGNNCC 2 cut(s) 147, 167
NmuCI GTSAC 1 cut(s) 41
PagI TCATGA 1 cut(s) 466
PfeI GAWTC 1 cut(s) 58
PkrI GCNGC 2 cut(s) 138, 406
PleI GAGTC 2 cut(s) 103, 471
PmaCI CACGTG 1 cut(s) 41
PmlI CACGTG 1 cut(s) 41
PpsI GAGTC 2 cut(s) 103, 471
Ppu21I YACGTR 2 cut(s) 41, 73
Psp124BI GAGCTC 1 cut(s) 128
PspCI CACGTG 1 cut(s) 41
PspN4I GGNNCC 2 cut(s) 147, 167
PspPI GGNCC 2 cut(s) 63, 90
PsrI GAACNNNNNNTAC 2 cut(s) 133, 165
PsuI RGATCY 1 cut(s) 145
SacI GAGCTC 1 cut(s) 128
SatI GCNGC 2 cut(s) 137, 405
Sau3AI GATC 1 cut(s) 145
Sau96I GGNCC 2 cut(s) 63, 90
SchI GAGTC 2 cut(s) 104, 472
SduI GDGCHC 2 cut(s) 128, 168
SetI ASST 7 cut(s) 43, 71, 75, 81, 128, 218, 431
SnaBI TACGTA 1 cut(s) 73
Sse9I AATT 1 cut(s) 392
SsiI CCGC 2 cut(s) 49, 136
SspI AATATT 1 cut(s) 420
SspMI CTAG 1 cut(s) 447
SstI GAGCTC 1 cut(s) 128
StyI CCWWGG 1 cut(s) 170
TaiI ACGT 2 cut(s) 43, 75
TasI AATT 1 cut(s) 392
TauI GCSGC 1 cut(s) 139
TfiI GAWTC 1 cut(s) 58
TscAI CASTG 1 cut(s) 159
TseFI GTSAC 1 cut(s) 41
TseI GCWGC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 41
TspDTI ATGAA 1 cut(s) 387
TspGWI ACGGA 1 cut(s) 158
TspRI CASTG 1 cut(s) 159
XapI RAATTY 1 cut(s) 392
XcmI CCANNNNNNNNNTGG 1 cut(s) 263
XspI CTAG 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.