MD12G1132300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012391
Physical Location & Seq
Forward (+)
20734355 .. 20734828
474 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1132300.v1.1.491

Sequence Viewer

Length: 474 bp
ATGACTTCAGTTTGCATATCAAACTGCGTCAACGACGCACGTGACCCCCGAGTGCCGATCCGGGCCACGTACGTGAACCTCTACAAGTGGCCTGAGTCGGACGCGGAGTTTGTGCGCTCGGTGAGCTCCAATGGGCGGCGAACTGATCCCGCGCAGCGACATGGGCACCCGAGGGTGGTGGACAGCATATCATGCAGGCAAATGTATCTCAGGAGCTACAAGTTTTCGAGGAAAGAGAGCGTGCCGGAGAAAACACAGAAGTGCTTTGGGAGAGTGAAGGAGAAAATGGCTGGAAATTACAATAGCAACGGGAATAAGGGTAAGAAGGAGAAGGGCAAAGTGGGGAGGAAAAAGAACGGTAGGTGTTTGGCTCTCAGGAAAATGAAGGAGTTTTCATCTGCTGCTCTCTTTAGAATATTTCAGAGGTTGCTGTCTTGCTCTGCTAGTATAGATGTTGTGAATCATGATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

17.91

Weight (kDa)

10.06

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012391)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02640
fragaria_vesca FvH4_6g14750
malus_domestica MD04G1116500.v1.1 MD12G1132300.v1.1
prunus_persica Prupe.4G261600_v2.0.a1 Prupe.4G261600_v2.0.a1 Prupe.6G246400_v2.0.a1
pyrus_communis pycom12g12820
rosa_chinensis RchiOBHm_Chr3g0467961
rosa_laevigata RLG00000024463
rosa_multiflora Rmu_sc0006017.1_g000003 Rmu_sc0034877.1_g000002
rosa_roxburghii Rroxscaffold_6G00413250
rosa_rugosa Rorug03G0090700
rosa_samantha Rh3AG139400 Rh3BG160900 Rh3CG160800 Rh3DG161400
rosa_wichuraiana Rw3G013190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 165
AccII CGCG 2 cut(s) 104, 152
AciI CCGC 3 cut(s) 104, 136, 150
AclWI GGATC 2 cut(s) 52, 140
AcvI CACGTG 1 cut(s) 41
AfaI GTAC 1 cut(s) 71
AfiI CCNNNNNNNGG 2 cut(s) 135, 175
AleI CACNNNNGTG 2 cut(s) 71, 259
AluBI AGCT 2 cut(s) 126, 216
AluI AGCT 2 cut(s) 126, 216
Alw21I GWGCWC 1 cut(s) 128
AlwI GGATC 2 cut(s) 52, 140
Ama87I CYCGRG 2 cut(s) 48, 169
AoxI GGCC 2 cut(s) 63, 89
ApeKI GCWGC 2 cut(s) 154, 401
ArsI GACNNNNNNTTYG 2 cut(s) 92, 124
AspLEI GCGC 2 cut(s) 117, 154
AspS9I GGNCC 1 cut(s) 63
AsuC2I CCSGG 1 cut(s) 62
AsuHPI GGTGA 1 cut(s) 133
AvaI CYCGRG 2 cut(s) 48, 169
BaeGI GKGCMC 1 cut(s) 168
BanI GGYRCC 1 cut(s) 165
BanII GRGCYC 1 cut(s) 128
BbrPI CACGTG 1 cut(s) 41
Bbv12I GWGCWC 1 cut(s) 128
BbvI GCAGC 2 cut(s) 166, 388
BcnI CCSGG 1 cut(s) 62
BfaI CTAG 1 cut(s) 444
BisI GCNGC 3 cut(s) 137, 155, 402
BlsI GCNGC 3 cut(s) 138, 156, 403
Bme1390I CCNGG 1 cut(s) 62
BmeT110I CYCGRG 2 cut(s) 48, 169
BmgT120I GGNCC 1 cut(s) 63
BmiI GGNNCC 1 cut(s) 167
BmrFI CCNGG 1 cut(s) 62
BpuMI CCSGG 1 cut(s) 62
BsaAI YACGTR 3 cut(s) 41, 69, 73
BsaJI CCNNGG 1 cut(s) 170
Bsc4I CCNNNNNNNGG 2 cut(s) 135, 175
BseDI CCNNGG 1 cut(s) 170
BseLI CCNNNNNNNGG 2 cut(s) 135, 175
BseMII CTCAG 3 cut(s) 84, 223, 388
BseSI GKGCMC 1 cut(s) 168
BseXI GCAGC 2 cut(s) 166, 388
Bsh1236I CGCG 2 cut(s) 104, 152
BshFI GGCC 2 cut(s) 65, 91
BshNI GGYRCC 1 cut(s) 165
BsiHKAI GWGCWC 1 cut(s) 128
BsiHKCI CYCGRG 2 cut(s) 48, 169
BsiSI CCGG 2 cut(s) 61, 245
BsiWI CGTACG 1 cut(s) 69
BslI CCNNNNNNNGG 2 cut(s) 135, 175
BsnI GGCC 2 cut(s) 65, 91
BsoBI CYCGRG 2 cut(s) 48, 169
Bsp1286I GDGCHC 2 cut(s) 128, 168
Bsp143I GATC 2 cut(s) 57, 145
BspACI CCGC 3 cut(s) 104, 136, 150
BspANI GGCC 2 cut(s) 65, 91
BspCNI CTCAG 3 cut(s) 85, 222, 387
BspFNI CGCG 2 cut(s) 104, 152
BspHI TCATGA 1 cut(s) 463
BspLI GGNNCC 1 cut(s) 167
BspPI GGATC 2 cut(s) 52, 140
BspT107I GGYRCC 1 cut(s) 165
BssECI CCNNGG 1 cut(s) 170
BssMI GATC 2 cut(s) 57, 145
Bst4CI ACNGT 1 cut(s) 359
BstAPI GCANNNNNTGC 1 cut(s) 192
BstBAI YACGTR 3 cut(s) 41, 69, 73
BstC8I GCNNGC 2 cut(s) 197, 242
BstDEI CTNAG 3 cut(s) 93, 209, 374
BstFNI CGCG 2 cut(s) 104, 152
BstHHI GCGC 2 cut(s) 117, 154
BstKTI GATC 2 cut(s) 60, 148
BstMBI GATC 2 cut(s) 57, 145
BstMWI GCNNNNNNNGC 3 cut(s) 123, 163, 192
BstSCI CCNGG 1 cut(s) 60
BstSLI GKGCMC 1 cut(s) 168
BstUI CGCG 2 cut(s) 104, 152
BstV1I GCAGC 2 cut(s) 166, 388
BsuRI GGCC 2 cut(s) 65, 91
Cac8I GCNNGC 2 cut(s) 197, 242
CciI TCATGA 1 cut(s) 463
CfoI GCGC 2 cut(s) 117, 154
Cfr13I GGNCC 1 cut(s) 63
CseI GACGC 3 cut(s) 16, 44, 110
Csp6I GTAC 1 cut(s) 70
CviAII CATG 3 cut(s) 161, 192, 464
CviJI RGCY 6 cut(s) 65, 91, 126, 216, 290, 371
CviKI_1 RGCY 6 cut(s) 65, 91, 126, 216, 290, 371
CviQI GTAC 1 cut(s) 70
DdeI CTNAG 3 cut(s) 93, 209, 374
DpnI GATC 2 cut(s) 59, 147
DpnII GATC 2 cut(s) 57, 145
Ecl136II GAGCTC 1 cut(s) 126
Eco24I GRGCYC 1 cut(s) 128
Eco53kI GAGCTC 1 cut(s) 126
Eco72I CACGTG 1 cut(s) 41
Eco88I CYCGRG 2 cut(s) 48, 169
EcoICRI GAGCTC 1 cut(s) 126
EcoT38I GRGCYC 1 cut(s) 128
FaeI CATG 3 cut(s) 164, 195, 467
FaiI YATR 6 cut(s) 17, 162, 188, 193, 449, 465
FatI CATG 3 cut(s) 160, 191, 463
FauI CCCGC 1 cut(s) 157
Fnu4HI GCNGC 3 cut(s) 137, 155, 402
FriOI GRGCYC 1 cut(s) 128
Fsp4HI GCNGC 3 cut(s) 137, 155, 402
FspBI CTAG 1 cut(s) 444
GlaI GCGC 2 cut(s) 116, 153
GluI GCNGC 3 cut(s) 137, 155, 402
HaeIII GGCC 2 cut(s) 65, 91
HapII CCGG 2 cut(s) 61, 245
HgaI GACGC 3 cut(s) 16, 44, 110
HhaI GCGC 2 cut(s) 117, 154
Hin1II CATG 3 cut(s) 164, 195, 467
Hin6I GCGC 2 cut(s) 115, 152
HinP1I GCGC 2 cut(s) 115, 152
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 2 cut(s) 95, 460
HpaII CCGG 2 cut(s) 61, 245
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 3 cut(s) 31, 76, 181
Hpy188I TCNGA 2 cut(s) 100, 423
Hpy188III TCNNGA 3 cut(s) 211, 376, 464
Hpy8I GTNNAC 3 cut(s) 31, 76, 181
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 4 cut(s) 271, 319, 325, 379
HpyCH4III ACNGT 1 cut(s) 359
HpyCH4IV ACGT 3 cut(s) 40, 68, 72
HpyCH4V TGCA 2 cut(s) 15, 195
HpyF10VI GCNNNNNNNGC 3 cut(s) 123, 163, 192
HpyF3I CTNAG 3 cut(s) 93, 209, 374
HpySE526I ACGT 3 cut(s) 40, 68, 72
Hsp92II CATG 3 cut(s) 164, 195, 467
HspAI GCGC 2 cut(s) 115, 152
Kzo9I GATC 2 cut(s) 57, 145
LmnI GCTCC 2 cut(s) 131, 213
LpnPI CCDG 7 cut(s) 74, 105, 181, 196, 258, 276, 361
Lsp1109I GCAGC 2 cut(s) 166, 388
MaeI CTAG 1 cut(s) 444
MaeII ACGT 3 cut(s) 40, 68, 72
MaeIII GTNAC 1 cut(s) 41
MalI GATC 2 cut(s) 59, 147
MboI GATC 2 cut(s) 57, 145
MhlI GDGCHC 2 cut(s) 128, 168
MluCI AATT 1 cut(s) 295
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 78
MnlI CCTC 5 cut(s) 89, 165, 222, 339, 417
MseI TTAA 1 cut(s) 472
MslI CAYNNNNRTG 2 cut(s) 71, 259
MspI CCGG 2 cut(s) 61, 245
MspR9I CCNGG 1 cut(s) 62
MvnI CGCG 2 cut(s) 104, 152
MwoI GCNNNNNNNGC 3 cut(s) 123, 163, 192
NciI CCSGG 1 cut(s) 62
NdeII GATC 2 cut(s) 57, 145
NlaIII CATG 3 cut(s) 164, 195, 467
NlaIV GGNNCC 1 cut(s) 167
NmuCI GTSAC 1 cut(s) 41
OliI CACNNNNGTG 2 cut(s) 71, 259
PagI TCATGA 1 cut(s) 463
PcsI WCGNNNNNNNCGW 1 cut(s) 46
PfeI GAWTC 1 cut(s) 460
Pfl23II CGTACG 1 cut(s) 69
PkrI GCNGC 3 cut(s) 138, 156, 403
PleI GAGTC 1 cut(s) 103
PmaCI CACGTG 1 cut(s) 41
PmlI CACGTG 1 cut(s) 41
PpsI GAGTC 1 cut(s) 103
Ppu21I YACGTR 3 cut(s) 41, 69, 73
Psp124BI GAGCTC 1 cut(s) 128
PspCI CACGTG 1 cut(s) 41
PspLI CGTACG 1 cut(s) 69
PspN4I GGNNCC 1 cut(s) 167
PspPI GGNCC 1 cut(s) 63
RsaI GTAC 1 cut(s) 71
RsaNI GTAC 1 cut(s) 70
RseI CAYNNNNRTG 2 cut(s) 71, 259
SacI GAGCTC 1 cut(s) 128
SaqAI TTAA 1 cut(s) 472
SatI GCNGC 3 cut(s) 137, 155, 402
Sau3AI GATC 2 cut(s) 57, 145
Sau96I GGNCC 1 cut(s) 63
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 1 cut(s) 62
SduI GDGCHC 2 cut(s) 128, 168
SetI ASST 8 cut(s) 43, 71, 75, 81, 128, 218, 365, 428
SmiMI CAYNNNNRTG 2 cut(s) 71, 259
Sse9I AATT 1 cut(s) 295
SsiI CCGC 3 cut(s) 104, 136, 150
SspI AATATT 1 cut(s) 417
SspMI CTAG 1 cut(s) 444
SstI GAGCTC 1 cut(s) 128
StyD4I CCNGG 1 cut(s) 60
TaaI ACNGT 1 cut(s) 359
TaiI ACGT 3 cut(s) 43, 71, 75
TaqI TCGA 1 cut(s) 227
TasI AATT 1 cut(s) 295
TauI GCSGC 1 cut(s) 139
TfiI GAWTC 1 cut(s) 460
Tru1I TTAA 1 cut(s) 472
Tru9I TTAA 1 cut(s) 472
TseFI GTSAC 1 cut(s) 41
TseI GCWGC 2 cut(s) 154, 401
Tsp45I GTSAC 1 cut(s) 41
TspDTI ATGAA 2 cut(s) 384, 398
XspI CTAG 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.