RLG00000024463

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0012391
Physical Location & Seq
Forward (+)
36041123 .. 36041593
471 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024463

Sequence Viewer

Length: 378 bp
ATGACCTCAGTTTGCATATCAAACTGTGTCAACGACACGCGCGATCCCCGCGTGCCGGTCCGGGCCACCTACGTCAACCTGTACAAGTGGCCTGAGTCGGACGCGGAGTTCGTGCGGTCGGTGAGCTCCAAAGGGAGGCGATCCAGTGCGGCGGGGATGCCGCATTGGCACCACCAACACCCAAGAGTGGTGGACAGCATATCATGCAGGCAAATGTACCTGAGGAGCTACACGTTCACGAGGAAAGAGACCGTGCCGGAGAAAACCCAGAAGTGCTTTGGCAGGGTCAAGGAGAAAATGGTAACTAACAACGATGAGAGGAAGAGGAAGAAGAAGAAGAAGGGTAAGAAGAATGTTGTGAATCATGATCAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

126

Amino Acids

14.65

Weight (kDa)

10.06

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012391)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02640
fragaria_vesca FvH4_6g14750
malus_domestica MD04G1116500.v1.1 MD12G1132300.v1.1
prunus_persica Prupe.4G261600_v2.0.a1 Prupe.4G261600_v2.0.a1 Prupe.6G246400_v2.0.a1
pyrus_communis pycom12g12820
rosa_chinensis RchiOBHm_Chr3g0467961
rosa_laevigata RLG00000024463
rosa_multiflora Rmu_sc0006017.1_g000003 Rmu_sc0034877.1_g000002
rosa_roxburghii Rroxscaffold_6G00413250
rosa_rugosa Rorug03G0090700
rosa_samantha Rh3AG139400 Rh3BG160900 Rh3CG160800 Rh3DG161400
rosa_wichuraiana Rw3G013190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 168
AccII CGCG 4 cut(s) 40, 42, 51, 104
AciI CCGC 6 cut(s) 49, 104, 115, 149, 152, 161
AclWI GGATC 2 cut(s) 38, 135
AfaI GTAC 2 cut(s) 83, 218
AfiI CCNNNNNNNGG 3 cut(s) 55, 135, 187
AflIII ACRYGT 1 cut(s) 231
AloI GAACNNNNNNTCC 2 cut(s) 92, 124
AluBI AGCT 2 cut(s) 126, 228
AluI AGCT 2 cut(s) 126, 228
Alw21I GWGCWC 1 cut(s) 128
Alw26I GTCTC 1 cut(s) 242
AlwI GGATC 2 cut(s) 38, 135
AoxI GGCC 2 cut(s) 63, 89
ArsI GACNNNNNNTTYG 2 cut(s) 92, 124
AspLEI GCGC 1 cut(s) 42
AspS9I GGNCC 2 cut(s) 58, 63
AsuC2I CCSGG 1 cut(s) 62
AsuHPI GGTGA 1 cut(s) 133
AvaII GGWCC 1 cut(s) 58
AxyI CCTNAGG 1 cut(s) 221
BanI GGYRCC 1 cut(s) 168
BanII GRGCYC 1 cut(s) 128
BauI CACGAG 1 cut(s) 238
Bbv12I GWGCWC 1 cut(s) 128
BclI TGATCA 1 cut(s) 367
BcnI CCSGG 1 cut(s) 62
BcoDI GTCTC 1 cut(s) 242
BglI GCCNNNNNGGC 1 cut(s) 166
BisI GCNGC 2 cut(s) 150, 161
BlsI GCNGC 2 cut(s) 151, 162
Bme1390I CCNGG 1 cut(s) 62
Bme18I GGWCC 1 cut(s) 58
BmgT120I GGNCC 2 cut(s) 58, 63
BmiI GGNNCC 1 cut(s) 170
BmrFI CCNGG 1 cut(s) 62
BmsI GCATC 1 cut(s) 147
BpuMI CCSGG 1 cut(s) 62
BsaI GGTCTC 1 cut(s) 242
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 135, 187
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 1 cut(s) 144
Bse21I CCTNAGG 1 cut(s) 221
BseGI GGATG 1 cut(s) 162
BseLI CCNNNNNNNGG 3 cut(s) 55, 135, 187
BseMII CTCAG 3 cut(s) 21, 84, 212
BseNI ACTGG 1 cut(s) 144
BseRI GAGGAG 1 cut(s) 238
Bsh1236I CGCG 4 cut(s) 40, 42, 51, 104
Bsh1285I CGRYCG 1 cut(s) 119
BshFI GGCC 2 cut(s) 65, 91
BshNI GGYRCC 1 cut(s) 168
BsiEI CGRYCG 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 128
BsiSI CCGG 3 cut(s) 56, 61, 257
BslI CCNNNNNNNGG 3 cut(s) 55, 135, 187
BsmAI GTCTC 1 cut(s) 242
BsnI GGCC 2 cut(s) 65, 91
Bso31I GGTCTC 1 cut(s) 242
Bsp1286I GDGCHC 1 cut(s) 128
Bsp1407I TGTACA 1 cut(s) 81
Bsp143I GATC 3 cut(s) 43, 140, 367
BspACI CCGC 6 cut(s) 49, 104, 115, 149, 152, 161
BspANI GGCC 2 cut(s) 65, 91
BspCNI CTCAG 3 cut(s) 20, 85, 213
BspFNI CGCG 4 cut(s) 40, 42, 51, 104
BspHI TCATGA 1 cut(s) 364
BspLI GGNNCC 1 cut(s) 170
BspPI GGATC 2 cut(s) 38, 135
BspT107I GGYRCC 1 cut(s) 168
BspTNI GGTCTC 1 cut(s) 242
BsrFI RCCGGY 1 cut(s) 55
BsrGI TGTACA 1 cut(s) 81
BsrI ACTGG 1 cut(s) 144
BssAI RCCGGY 1 cut(s) 55
BssMI GATC 3 cut(s) 43, 140, 367
BssSI CACGAG 1 cut(s) 238
Bst2BI CACGAG 1 cut(s) 238
Bst4CI ACNGT 2 cut(s) 26, 253
Bst6I CTCTTC 1 cut(s) 317
BstAPI GCANNNNNTGC 1 cut(s) 204
BstAUI TGTACA 1 cut(s) 81
BstC8I GCNNGC 2 cut(s) 53, 209
BstDEI CTNAG 3 cut(s) 7, 93, 221
BstF5I GGATG 1 cut(s) 162
BstFNI CGCG 4 cut(s) 40, 42, 51, 104
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 3 cut(s) 46, 143, 370
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 3 cut(s) 43, 140, 367
BstMCI CGRYCG 1 cut(s) 119
BstMWI GCNNNNNNNGC 3 cut(s) 48, 166, 204
BstSCI CCNGG 1 cut(s) 60
BstUI CGCG 4 cut(s) 40, 42, 51, 104
Bsu36I CCTNAGG 1 cut(s) 221
BsuRI GGCC 2 cut(s) 65, 91
BtsCI GGATG 1 cut(s) 162
BtsIMutI CAGTG 1 cut(s) 151
Cac8I GCNNGC 2 cut(s) 53, 209
CciI TCATGA 1 cut(s) 364
CfoI GCGC 1 cut(s) 42
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 2 cut(s) 58, 63
CpoI CGGWCCG 1 cut(s) 58
CseI GACGC 1 cut(s) 110
Csp6I GTAC 2 cut(s) 82, 217
CspCI CAANNNNNGTGG 2 cut(s) 171, 206
CspI CGGWCCG 1 cut(s) 58
CviAII CATG 2 cut(s) 204, 365
CviJI RGCY 4 cut(s) 65, 91, 126, 228
CviKI_1 RGCY 4 cut(s) 65, 91, 126, 228
CviQI GTAC 2 cut(s) 82, 217
DdeI CTNAG 3 cut(s) 7, 93, 221
DpnI GATC 3 cut(s) 45, 142, 369
DpnII GATC 3 cut(s) 43, 140, 367
Eam1104I CTCTTC 1 cut(s) 317
EarI CTCTTC 1 cut(s) 317
Ecl136II GAGCTC 1 cut(s) 126
Eco24I GRGCYC 1 cut(s) 128
Eco31I GGTCTC 1 cut(s) 242
Eco47I GGWCC 1 cut(s) 58
Eco53kI GAGCTC 1 cut(s) 126
Eco81I CCTNAGG 1 cut(s) 221
EcoICRI GAGCTC 1 cut(s) 126
EcoT38I GRGCYC 1 cut(s) 128
FaeI CATG 2 cut(s) 207, 368
FaiI YATR 4 cut(s) 17, 200, 205, 366
FatI CATG 2 cut(s) 203, 364
FauI CCCGC 2 cut(s) 56, 145
FbaI TGATCA 1 cut(s) 367
Fnu4HI GCNGC 2 cut(s) 150, 161
FokI GGATG 1 cut(s) 169
FriOI GRGCYC 1 cut(s) 128
Fsp4HI GCNGC 2 cut(s) 150, 161
GlaI GCGC 1 cut(s) 41
GluI GCNGC 2 cut(s) 150, 161
HaeIII GGCC 2 cut(s) 65, 91
HapII CCGG 3 cut(s) 56, 61, 257
HgaI GACGC 1 cut(s) 110
HhaI GCGC 1 cut(s) 42
Hin1II CATG 2 cut(s) 207, 368
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HincII GTYRAC 2 cut(s) 31, 76
HindII GTYRAC 2 cut(s) 31, 76
HinfI GANTC 2 cut(s) 95, 361
HpaII CCGG 3 cut(s) 56, 61, 257
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 4 cut(s) 31, 76, 193, 237
Hpy188I TCNGA 1 cut(s) 100
Hpy188III TCNNGA 3 cut(s) 238, 365, 371
Hpy8I GTNNAC 4 cut(s) 31, 76, 193, 237
HpyAV CCTTC 1 cut(s) 334
HpyCH4III ACNGT 2 cut(s) 26, 253
HpyCH4IV ACGT 2 cut(s) 72, 233
HpyCH4V TGCA 2 cut(s) 15, 207
HpyF10VI GCNNNNNNNGC 3 cut(s) 48, 166, 204
HpyF3I CTNAG 3 cut(s) 7, 93, 221
HpySE526I ACGT 2 cut(s) 72, 233
Hsp92II CATG 2 cut(s) 207, 368
HspAI GCGC 1 cut(s) 40
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 3 cut(s) 43, 140, 367
LmnI GCTCC 2 cut(s) 131, 225
LweI GCATC 1 cut(s) 147
MaeII ACGT 2 cut(s) 72, 233
MaeIII GTNAC 1 cut(s) 301
MalI GATC 3 cut(s) 45, 142, 369
MboI GATC 3 cut(s) 43, 140, 367
MboII GAAGA 6 cut(s) 334, 340, 343, 346, 349, 361
MhlI GDGCHC 1 cut(s) 128
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 78
MnlI CCTC 6 cut(s) 16, 129, 216, 234, 312, 318
MseI TTAA 1 cut(s) 376
MspI CCGG 3 cut(s) 56, 61, 257
MspR9I CCNGG 1 cut(s) 62
MvnI CGCG 4 cut(s) 40, 42, 51, 104
MwoI GCNNNNNNNGC 3 cut(s) 48, 166, 204
NciI CCSGG 1 cut(s) 62
NdeII GATC 3 cut(s) 43, 140, 367
NlaIII CATG 2 cut(s) 207, 368
NlaIV GGNNCC 1 cut(s) 170
PagI TCATGA 1 cut(s) 364
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 108
PfeI GAWTC 1 cut(s) 361
PkrI GCNGC 2 cut(s) 151, 162
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
Psp124BI GAGCTC 1 cut(s) 128
PspN4I GGNNCC 1 cut(s) 170
PspPI GGNCC 2 cut(s) 58, 63
RsaI GTAC 2 cut(s) 83, 218
RsaNI GTAC 2 cut(s) 82, 217
Rsr2I CGGWCCG 1 cut(s) 58
RsrII CGGWCCG 1 cut(s) 58
SacI GAGCTC 1 cut(s) 128
SaqAI TTAA 1 cut(s) 376
SatI GCNGC 2 cut(s) 150, 161
Sau3AI GATC 3 cut(s) 43, 140, 367
Sau96I GGNCC 2 cut(s) 58, 63
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 1 cut(s) 62
SduI GDGCHC 1 cut(s) 128
SetI ASST 8 cut(s) 8, 71, 75, 81, 128, 222, 230, 236
SfaNI GCATC 1 cut(s) 147
SinI GGWCC 1 cut(s) 58
SsiI CCGC 6 cut(s) 49, 104, 115, 149, 152, 161
SstI GAGCTC 1 cut(s) 128
StyD4I CCNGG 1 cut(s) 60
TaaI ACNGT 2 cut(s) 26, 253
TaiI ACGT 2 cut(s) 75, 236
TatI WGTACW 1 cut(s) 81
TauI GCSGC 2 cut(s) 152, 163
TfiI GAWTC 1 cut(s) 361
Tru1I TTAA 1 cut(s) 376
Tru9I TTAA 1 cut(s) 376
TscAI CASTG 1 cut(s) 151
TspRI CASTG 1 cut(s) 151
VpaK11BI GGWCC 1 cut(s) 58
XcmI CCANNNNNNNNNTGG 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.