MD04G1117200.v1.1

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0015652
Physical Location & Seq
Forward (+)
20231121 .. 20232203
1083 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1117200.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGGCCAGAGGCCAAGCTCCAGGTGATCATAGTTCAGATGAAGACTACATAGACATGGAAGTGAGCTCTTCCTCCAACTTCTTGTGCTATACCATCAGCTCTCCACCACAAACCAGAGAGTTTGAGTTCCAGATGTCCTCACTTTGTCAAGACAAAGAAACCACAAATTCCCCAGCTGATGAACTCTTCTACAAAGGTAAGCTCCTCCCGCTCCACCTCCCACCTCGCCTGCAAATGGTCCAAAAGATCCTCCAAACCTCCAACAACAAAACACAGGAAGCCATTGACGAAAGCTTCACCAAAAGGGAAAGCTCCAATACCCCATTGGACCAGTCCTACAATATCGCCCCCTCAGAGTCCTGCAGGGTCAGCAGTGAACTCAACTCAGATGAGTACATCTCCGAATGGACGGCTGACATGTGTGTGAATTTCATTGGTGGGCACAATGATCATCAAAAGAAATCTTGGTCCACAAAGCTGAAGCAGATCAAGCAGTCCTCATTGGGTCAAAAGCTCAAGGCTTCAAGGGCATATGTGAAGTCCCTGTTCAGAAAAACCACCTGTGCGGATGACTACAACTCATGTGCAGAAGCAGAAAGTGGCTCAAAAGTCAGAGACTTTTGCTCCAACAAGTACATGAACGTGGCTGAGAAAACCCCATTTGGAAGAGTCGACGGTGGCAGATTCCAAATATCATCAACCACTCTCATGAAAAGCATTGAGAAAGAAATGGCCGAGGAAAACTCAAACACCCACAGAAAATCTTTCTCTGGGGCAATTCAACATACAACTGCCCTGCAATCTCTGTGTTCACCTACATCTTCCTCCGTTTCTTCGTCTTCCTCGTCTTCTTTCTCCTTCAGCTCAAGTGGGTTCTCAGATTTGCAGCTGCTGAAGCGAAGTACGAGTGCAAATTCAGAGCTTGAGGGCTCAATCGAGGGGGCAATTGCTCATTGCAAACAGTCCCAGCAGCTGTTGAGTTCAAGAATGCAGTTTGTGGGGGTGAAGAGATCTCACCAGGGTTCGTCCAATAATCCTATGAGAGACGCACACATGAATAATTTGATTGCATTATATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

361

Amino Acids

39.92

Weight (kDa)

6.52

Isoelectric Point (pI)

58.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015652)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g14700
malus_domestica MD04G1117200.v1.1 MD12G1132800.v1.1
prunus_persica Prupe.6G246800_v2.0.a1
pyrus_communis pycom04g10690
rosa_chinensis RchiOBHm_Chr3g0467911
rosa_laevigata RLG00000024468
rosa_multiflora Rmu_sc0001793.1_g000046 Rmu_sc0001793.1_g000047
rosa_roxburghii Rroxscaffold_6G00413320
rosa_rugosa Rorug03G0090500
rosa_samantha Rh3AG138800 Rh3BG160400 Rh3CG160400 Rh3DG161000
rosa_wichuraiana Rw3G013150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 211
AccI GTMKAC 1 cut(s) 672
AciI CCGC 2 cut(s) 209, 566
AclWI GGATC 1 cut(s) 241
AcoI YGGCCR 2 cut(s) 3, 732
AcsI RAATTY 3 cut(s) 166, 427, 913
AcuI CTGAAG 3 cut(s) 500, 844, 914
AfaI GTAC 3 cut(s) 395, 635, 904
AfiI CCNNNNNNNGG 1 cut(s) 235
AflIII ACRYGT 1 cut(s) 417
AgsI TTSAA 3 cut(s) 525, 782, 984
AjnI CCWGG 2 cut(s) 19, 1017
Alw21I GWGCWC 1 cut(s) 68
Alw26I GTCTC 2 cut(s) 609, 1038
AlwI GGATC 1 cut(s) 241
AlwNI CAGNNNCTG 2 cut(s) 892, 973
AoxI GGCC 3 cut(s) 3, 10, 732
ApeKI GCWGC 3 cut(s) 886, 889, 970
ApoI RAATTY 3 cut(s) 166, 427, 913
AspS9I GGNCC 3 cut(s) 238, 328, 468
AsuHPI GGTGA 5 cut(s) 35, 289, 804, 1007, 1015
AvaII GGWCC 3 cut(s) 238, 328, 468
BaeGI GKGCMC 1 cut(s) 444
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 2 cut(s) 68, 932
BbsI GAAGAC 3 cut(s) 48, 831, 840
Bbv12I GWGCWC 1 cut(s) 68
BbvI GCAGC 3 cut(s) 876, 898, 982
BccI CCATC 1 cut(s) 101
BceAI ACGGC 1 cut(s) 426
BciT130I CCWGG 2 cut(s) 21, 1019
BclI TGATCA 2 cut(s) 25, 448
BcoDI GTCTC 2 cut(s) 609, 1038
BfmI CTRYAG 1 cut(s) 361
BglII AGATCT 1 cut(s) 1010
BisI GCNGC 3 cut(s) 887, 890, 971
BlsI GCNGC 3 cut(s) 888, 891, 972
Bme1390I CCNGG 2 cut(s) 21, 1019
Bme18I GGWCC 3 cut(s) 238, 328, 468
BmgT120I GGNCC 3 cut(s) 238, 328, 468
BmrFI CCNGG 2 cut(s) 21, 1019
BpiI GAAGAC 3 cut(s) 48, 831, 840
BplI GAGNNNNNCTC 4 cut(s) 383, 415, 728, 760
BpuEI CTTGAG 3 cut(s) 500, 850, 944
BsaJI CCNNGG 2 cut(s) 735, 1018
BsaXI ACNNNNNCTCC 2 cut(s) 608, 638
Bsc4I CCNNNNNNNGG 1 cut(s) 235
Bse1I ACTGG 1 cut(s) 331
Bse3DI GCAATG 1 cut(s) 952
BseBI CCWGG 2 cut(s) 21, 1019
BseDI CCNNGG 2 cut(s) 735, 1018
BseGI GGATG 1 cut(s) 574
BseLI CCNNNNNNNGG 1 cut(s) 235
BseMI GCAATG 1 cut(s) 952
BseMII CTCAG 4 cut(s) 366, 399, 639, 891
BseNI ACTGG 1 cut(s) 331
BseRI GAGGAG 1 cut(s) 194
BseSI GKGCMC 1 cut(s) 444
BseXI GCAGC 3 cut(s) 876, 898, 982
BseYI CCCAGC 2 cut(s) 172, 966
BsgI GTGCAG 1 cut(s) 606
BshFI GGCC 3 cut(s) 5, 12, 734
BsiHKAI GWGCWC 1 cut(s) 68
BslFI GGGAC 2 cut(s) 526, 949
BslI CCNNNNNNNGG 1 cut(s) 235
BsmAI GTCTC 2 cut(s) 609, 1038
BsmBI CGTCTC 1 cut(s) 1038
BsmFI GGGAC 2 cut(s) 526, 949
BsmI GAATGC 1 cut(s) 993
BsnI GGCC 3 cut(s) 5, 12, 734
Bsp1286I GDGCHC 3 cut(s) 68, 444, 932
Bsp143I GATC 5 cut(s) 25, 246, 448, 486, 1010
BspACI CCGC 2 cut(s) 209, 566
BspANI GGCC 3 cut(s) 5, 12, 734
BspCNI CTCAG 4 cut(s) 365, 398, 640, 890
BspHI TCATGA 1 cut(s) 708
BspMAI CTGCAG 1 cut(s) 365
BspPI GGATC 1 cut(s) 241
BspQI GCTCTTC 1 cut(s) 73
BsrBI CCGCTC 1 cut(s) 211
BsrDI GCAATG 1 cut(s) 952
BsrI ACTGG 1 cut(s) 331
BssECI CCNNGG 2 cut(s) 735, 1018
BssMI GATC 5 cut(s) 25, 246, 448, 486, 1010
Bst2UI CCWGG 2 cut(s) 21, 1019
Bst4CI ACNGT 2 cut(s) 677, 963
Bst6I CTCTTC 4 cut(s) 73, 191, 661, 1001
BstC8I GCNNGC 1 cut(s) 230
BstDEI CTNAG 4 cut(s) 352, 385, 648, 877
BstF5I GGATG 1 cut(s) 574
BstKTI GATC 5 cut(s) 28, 249, 451, 489, 1013
BstMAI GTCTC 2 cut(s) 609, 1038
BstMBI GATC 5 cut(s) 25, 246, 448, 486, 1010
BstMWI GCNNNNNNNGC 5 cut(s) 208, 369, 490, 527, 895
BstNI CCWGG 2 cut(s) 21, 1019
BstNSI RCATGY 1 cut(s) 421
BstSCI CCNGG 2 cut(s) 19, 1017
BstSFI CTRYAG 1 cut(s) 361
BstSLI GKGCMC 1 cut(s) 444
BstV1I GCAGC 3 cut(s) 876, 898, 982
BstV2I GAAGAC 3 cut(s) 48, 831, 840
BstX2I RGATCY 2 cut(s) 246, 1010
BstYI RGATCY 2 cut(s) 246, 1010
BsuRI GGCC 3 cut(s) 5, 12, 734
BtsCI GGATG 1 cut(s) 574
BtsI GCAGTG 1 cut(s) 379
BtsIMutI CAGTG 1 cut(s) 379
Cac8I GCNNGC 1 cut(s) 230
CaiI CAGNNNCTG 2 cut(s) 892, 973
CciI TCATGA 1 cut(s) 708
Cfr13I GGNCC 3 cut(s) 238, 328, 468
CseI GACGC 1 cut(s) 1055
Csp6I GTAC 3 cut(s) 394, 634, 903
CviAII CATG 6 cut(s) 55, 418, 582, 637, 709, 1054
CviQI GTAC 3 cut(s) 394, 634, 903
DdeI CTNAG 4 cut(s) 352, 385, 648, 877
DpnI GATC 5 cut(s) 27, 248, 450, 488, 1012
DpnII GATC 5 cut(s) 25, 246, 448, 486, 1010
EaeI YGGCCR 2 cut(s) 3, 732
Eam1104I CTCTTC 4 cut(s) 73, 191, 661, 1001
EarI CTCTTC 4 cut(s) 73, 191, 661, 1001
Ecl136II GAGCTC 1 cut(s) 66
Eco24I GRGCYC 2 cut(s) 68, 932
Eco47I GGWCC 3 cut(s) 238, 328, 468
Eco53kI GAGCTC 1 cut(s) 66
Eco57I CTGAAG 3 cut(s) 500, 844, 914
EcoICRI GAGCTC 1 cut(s) 66
EcoRII CCWGG 2 cut(s) 19, 1017
EcoT38I GRGCYC 2 cut(s) 68, 932
Esp3I CGTCTC 1 cut(s) 1038
FaeI CATG 6 cut(s) 58, 421, 585, 640, 712, 1057
FaqI GGGAC 2 cut(s) 526, 949
FatI CATG 6 cut(s) 54, 417, 581, 636, 708, 1053
FauI CCCGC 1 cut(s) 216
FauNDI CATATG 1 cut(s) 532
FbaI TGATCA 2 cut(s) 25, 448
FblI GTMKAC 1 cut(s) 672
Fnu4HI GCNGC 3 cut(s) 887, 890, 971
FokI GGATG 1 cut(s) 581
FriOI GRGCYC 2 cut(s) 68, 932
Fsp4HI GCNGC 3 cut(s) 887, 890, 971
GluI GCNGC 3 cut(s) 887, 890, 971
GsaI CCCAGC 2 cut(s) 176, 970
HaeIII GGCC 3 cut(s) 5, 12, 734
HgaI GACGC 1 cut(s) 1055
Hin1II CATG 6 cut(s) 58, 421, 585, 640, 712, 1057
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HindIII AAGCTT 1 cut(s) 292
HinfI GANTC 3 cut(s) 356, 669, 684
HphI GGTGA 5 cut(s) 35, 289, 804, 1007, 1015
Hpy166II GTNNAC 4 cut(s) 377, 471, 673, 812
Hpy188I TCNGA 8 cut(s) 37, 355, 388, 403, 551, 614, 880, 919
Hpy188III TCNNGA 4 cut(s) 130, 149, 709, 984
Hpy8I GTNNAC 4 cut(s) 377, 471, 673, 812
Hpy99I CGWCG 1 cut(s) 677
HpyAV CCTTC 1 cut(s) 868
HpyCH4III ACNGT 2 cut(s) 677, 963
HpyCH4IV ACGT 1 cut(s) 642
HpyCH4V TGCA 9 cut(s) 232, 363, 587, 799, 886, 911, 957, 991, 1070
HpyF10VI GCNNNNNNNGC 5 cut(s) 208, 369, 490, 527, 895
HpyF3I CTNAG 4 cut(s) 352, 385, 648, 877
HpySE526I ACGT 1 cut(s) 642
Hsp92II CATG 6 cut(s) 58, 421, 585, 640, 712, 1057
Ksp22I TGATCA 2 cut(s) 25, 448
Kzo9I GATC 5 cut(s) 25, 246, 448, 486, 1010
LguI GCTCTTC 1 cut(s) 73
LmnI GCTCC 5 cut(s) 22, 207, 216, 317, 629
Lsp1109I GCAGC 3 cut(s) 876, 898, 982
MaeII ACGT 1 cut(s) 642
MalI GATC 5 cut(s) 27, 248, 450, 488, 1012
MbiI CCGCTC 1 cut(s) 211
MboI GATC 5 cut(s) 25, 246, 448, 486, 1010
MboII GAAGA 9 cut(s) 53, 60, 178, 678, 813, 825, 831, 840, 1018
MfeI CAATTG 1 cut(s) 945
MflI RGATCY 2 cut(s) 246, 1010
MhlI GDGCHC 3 cut(s) 68, 444, 932
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 166, 427, 777, 913, 945, 1060
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 365, 678
MmeI TCCRAC 3 cut(s) 99, 285, 651
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 5 cut(s) 53, 59, 422, 641, 707
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 176, 889, 973
MspR9I CCNGG 2 cut(s) 21, 1019
MunI CAATTG 1 cut(s) 945
Mva1269I GAATGC 1 cut(s) 993
MvaI CCWGG 2 cut(s) 21, 1019
MwoI GCNNNNNNNGC 5 cut(s) 208, 369, 490, 527, 895
NdeI CATATG 1 cut(s) 532
NdeII GATC 5 cut(s) 25, 246, 448, 486, 1010
NlaIII CATG 6 cut(s) 58, 421, 585, 640, 712, 1057
NmeAIII GCCGAG 1 cut(s) 760
NspI RCATGY 1 cut(s) 421
PagI TCATGA 1 cut(s) 708
PciI ACATGT 1 cut(s) 417
PciSI GCTCTTC 1 cut(s) 73
PcsI WCGNNNNNNNCGW 1 cut(s) 842
PctI GAATGC 1 cut(s) 993
PfeI GAWTC 1 cut(s) 684
PkrI GCNGC 3 cut(s) 888, 891, 972
PleI GAGTC 2 cut(s) 364, 677
PpsI GAGTC 2 cut(s) 364, 677
PscI ACATGT 1 cut(s) 417
Psp124BI GAGCTC 1 cut(s) 68
Psp6I CCWGG 2 cut(s) 19, 1017
PspFI CCCAGC 2 cut(s) 172, 966
PspGI CCWGG 2 cut(s) 19, 1017
PspPI GGNCC 3 cut(s) 238, 328, 468
PstI CTGCAG 1 cut(s) 365
PstNI CAGNNNCTG 2 cut(s) 892, 973
PsuI RGATCY 2 cut(s) 246, 1010
PvuII CAGCTG 3 cut(s) 176, 889, 973
RsaI GTAC 3 cut(s) 395, 635, 904
RsaNI GTAC 3 cut(s) 394, 634, 903
RseI CAYNNNNRTG 5 cut(s) 53, 59, 422, 641, 707
SacI GAGCTC 1 cut(s) 68
SalI GTCGAC 1 cut(s) 671
SapI GCTCTTC 1 cut(s) 73
SatI GCNGC 3 cut(s) 887, 890, 971
Sau3AI GATC 5 cut(s) 25, 246, 448, 486, 1010
Sau96I GGNCC 3 cut(s) 238, 328, 468
SbfI CCTGCAGG 1 cut(s) 365
SchI GAGTC 2 cut(s) 365, 678
ScrFI CCNGG 2 cut(s) 21, 1019
SdaI CCTGCAGG 1 cut(s) 365
SduI GDGCHC 3 cut(s) 68, 444, 932
SfcI CTRYAG 1 cut(s) 361
SinI GGWCC 3 cut(s) 238, 328, 468
SmiMI CAYNNNNRTG 5 cut(s) 53, 59, 422, 641, 707
SmlI CTYRAG 3 cut(s) 515, 865, 923
SmoI CTYRAG 3 cut(s) 515, 865, 923
Sse8387I CCTGCAGG 1 cut(s) 365
Sse9I AATT 6 cut(s) 166, 427, 777, 913, 945, 1060
SsiI CCGC 2 cut(s) 209, 566
SstI GAGCTC 1 cut(s) 68
StyD4I CCNGG 2 cut(s) 19, 1017
TaaI ACNGT 2 cut(s) 677, 963
TaiI ACGT 1 cut(s) 645
TaqI TCGA 2 cut(s) 672, 936
TasI AATT 6 cut(s) 166, 427, 777, 913, 945, 1060
TatI WGTACW 2 cut(s) 393, 633
TfiI GAWTC 1 cut(s) 684
TscAI CASTG 1 cut(s) 379
TseI GCWGC 3 cut(s) 886, 889, 970
TspDTI ATGAA 6 cut(s) 54, 195, 421, 653, 725, 1070
TspGWI ACGGA 1 cut(s) 817
TspRI CASTG 1 cut(s) 379
VpaK11BI GGWCC 3 cut(s) 238, 328, 468
XapI RAATTY 3 cut(s) 166, 427, 913
XceI RCATGY 1 cut(s) 421
XcmI CCANNNNNNNNNTGG 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.