MD12G1132800.v1.1

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015652
Physical Location & Seq
Forward (+)
20822701 .. 20823828
1128 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1132800.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGGCCAGAAGCCAAGCTTCAGGTGATCAAAGTGCGGATGAAGACTACATAGACATAGAAGTGAGCTCTTCCTCCAACTTCTTGTGCTATTCCATCAGCTCTCCACCACAAACCAGAGAGTTTGAGTTCCAAATGTCCTCACTTTCTCAAGACAAAGAAACTACAACATCCCCAGCTGATGAACTCTTCTACAAAGGTACGCTCCTCCCTCTCCACCTCCCACCTCGCCTGCAAATGGTCCAAAAGATCCTCCAAACCTCCAACAACAAAACCCAGGAAGCCATGGGTGAAAGCTTCACCAAAAGGGAAAGCTCCAACACCCCATTGGATCAATCCTGCAATATCTCCTCCTCAGAGTCCTGCAGGGTCAGCAGTGAGCTCAACTCAGATGAGTACATCTCCGAATGGACGGCTGACATGTGTGTGAATTTCATTGGTGGTCACAATGATCATCCAAAGAAATCTTGGTCCACAAAGCTGAAGCAGATCAAGCAGTCCTCATTGGGTCAAAAGCTCAAGGCTTCAAGGGCGTATGTGAAGTCCCTGTTCACAAAACCCACCTGTGCAGATGACTACAACTCATGTGCCAAACCAGCAAGCAATGCAGAAGCAGAAGCAGAAAATGGCTCCAAAGTCACAGACTTTTGCTCCAACAAGTACATGAAAGTGGCCAAGAAAACCCCATTTGGAAGAATCGACAATGGCAGGTTCCAAATATCATCCACCACTCTGATGAAAAGCATTGAGAAAGAAATGGCTGAGGAAAACGCAAACACCCACAGAAAATCTTTCTCTGGGGCAATCCAACATACACCTGCTCTGCAATCTCTGTGTTCCCCCACATCTTCCTCTGTTTCTTCGTCTTCCTCGTCTTCTTTCTCCTTCACCTCAAGTGGGTTAACAGATTTGCAGCTGCTGAAGCGAAGTACGAGTGCAAATTCAGAACTTGAGAGCTCGATAGAAGGAGCAATTGCTTATTGCAAACAGTCCCACCAGCTGGTCAGTTCAAGAATGCAGTTTGTGGGGGTCAAGAGATCTCACCAGGCCTCATCCAATAACGCCATGAGAGAAACACACATGAATAATTTGATTGCGTTATATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

369

Amino Acids

40.52

Weight (kDa)

6.72

Isoelectric Point (pI)

61.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015652)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g14700
malus_domestica MD04G1117200.v1.1 MD12G1132800.v1.1
prunus_persica Prupe.6G246800_v2.0.a1
pyrus_communis pycom04g10690
rosa_chinensis RchiOBHm_Chr3g0467911
rosa_laevigata RLG00000024468
rosa_multiflora Rmu_sc0001793.1_g000046 Rmu_sc0001793.1_g000047
rosa_roxburghii Rroxscaffold_6G00413320
rosa_rugosa Rorug03G0090500
rosa_samantha Rh3AG138800 Rh3BG160400 Rh3CG160400 Rh3DG161000
rosa_wichuraiana Rw3G013150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 823
Acc36I ACCTGC 2 cut(s) 696, 823
AccB7I CCANNNNNTGG 1 cut(s) 997
AciI CCGC 1 cut(s) 35
AclWI GGATC 2 cut(s) 241, 336
AcoI YGGCCR 2 cut(s) 3, 669
AcsI RAATTY 2 cut(s) 427, 937
AcuI CTGAAG 2 cut(s) 500, 938
AfaI GTAC 4 cut(s) 199, 395, 659, 928
AfiI CCNNNNNNNGG 3 cut(s) 235, 894, 997
AflIII ACRYGT 1 cut(s) 417
AgsI TTSAA 2 cut(s) 525, 1008
AjnI CCWGG 2 cut(s) 273, 1041
Alw21I GWGCWC 3 cut(s) 68, 381, 956
AlwI GGATC 2 cut(s) 241, 336
AlwNI CAGNNNCTG 1 cut(s) 916
AoxI GGCC 3 cut(s) 3, 669, 1044
ApeKI GCWGC 2 cut(s) 910, 913
ApoI RAATTY 2 cut(s) 427, 937
AspS9I GGNCC 2 cut(s) 238, 468
AsuHPI GGTGA 5 cut(s) 35, 289, 299, 877, 1031
AvaII GGWCC 2 cut(s) 238, 468
BalI TGGCCA 2 cut(s) 5, 671
BanII GRGCYC 3 cut(s) 68, 381, 956
BbsI GAAGAC 3 cut(s) 48, 855, 864
Bbv12I GWGCWC 3 cut(s) 68, 381, 956
BbvCI CCTCAGC 1 cut(s) 759
BbvI GCAGC 2 cut(s) 900, 922
BccI CCATC 1 cut(s) 101
BceAI ACGGC 1 cut(s) 426
BciT130I CCWGG 2 cut(s) 275, 1043
BclI TGATCA 2 cut(s) 25, 448
BfmI CTRYAG 1 cut(s) 361
BfuAI ACCTGC 2 cut(s) 696, 823
BglII AGATCT 1 cut(s) 1034
BisI GCNGC 2 cut(s) 911, 914
BlsI GCNGC 2 cut(s) 912, 915
Bme1390I CCNGG 2 cut(s) 275, 1043
Bme18I GGWCC 2 cut(s) 238, 468
BmgT120I GGNCC 2 cut(s) 238, 468
BmiI GGNNCC 2 cut(s) 628, 710
BmrFI CCNGG 2 cut(s) 275, 1043
BpiI GAAGAC 3 cut(s) 48, 855, 864
BplI GAGNNNNNCTC 4 cut(s) 368, 400, 383, 415
Bpu10I CCTNAGC 1 cut(s) 759
BpuEI CTTGAG 4 cut(s) 132, 500, 874, 968
BsaJI CCNNGG 2 cut(s) 273, 282
BsaXI ACNNNNNCTCC 2 cut(s) 632, 662
Bsc4I CCNNNNNNNGG 3 cut(s) 235, 894, 997
Bse3DI GCAATG 1 cut(s) 607
BseBI CCWGG 2 cut(s) 275, 1043
BseDI CCNNGG 2 cut(s) 273, 282
BseGI GGATG 5 cut(s) 43, 167, 451, 719, 1049
BseLI CCNNNNNNNGG 3 cut(s) 235, 894, 997
BseMI GCAATG 1 cut(s) 607
BseMII CTCAG 3 cut(s) 366, 399, 750
BseRI GAGGAG 3 cut(s) 194, 337, 340
BseXI GCAGC 2 cut(s) 900, 922
BseYI CCCAGC 1 cut(s) 172
BsgI GTGCAG 1 cut(s) 585
BshFI GGCC 3 cut(s) 5, 671, 1046
BsiHKAI GWGCWC 3 cut(s) 68, 381, 956
BslFI GGGAC 2 cut(s) 526, 973
BslI CCNNNNNNNGG 3 cut(s) 235, 894, 997
BsmFI GGGAC 2 cut(s) 526, 973
BsmI GAATGC 1 cut(s) 1017
BsnI GGCC 3 cut(s) 5, 671, 1046
Bsp1286I GDGCHC 3 cut(s) 68, 381, 956
Bsp143I GATC 6 cut(s) 25, 246, 328, 448, 486, 1034
Bsp19I CCATGG 1 cut(s) 282
BspACI CCGC 1 cut(s) 35
BspANI GGCC 3 cut(s) 5, 671, 1046
BspCNI CTCAG 3 cut(s) 365, 398, 751
BspLI GGNNCC 2 cut(s) 628, 710
BspMAI CTGCAG 1 cut(s) 365
BspMI ACCTGC 2 cut(s) 696, 823
BspPI GGATC 2 cut(s) 241, 336
BspQI GCTCTTC 1 cut(s) 73
BsrDI GCAATG 1 cut(s) 607
BssECI CCNNGG 2 cut(s) 273, 282
BssMI GATC 6 cut(s) 25, 246, 328, 448, 486, 1034
BssT1I CCWWGG 1 cut(s) 282
Bst2UI CCWGG 2 cut(s) 275, 1043
Bst4CI ACNGT 1 cut(s) 987
Bst6I CTCTTC 2 cut(s) 73, 191
BstAPI GCANNNNNTGC 1 cut(s) 602
BstC8I GCNNGC 2 cut(s) 230, 598
BstDEI CTNAG 3 cut(s) 352, 385, 759
BstDSI CCRYGG 1 cut(s) 282
BstF5I GGATG 5 cut(s) 43, 167, 451, 719, 1049
BstKTI GATC 6 cut(s) 28, 249, 331, 451, 489, 1037
BstMBI GATC 6 cut(s) 25, 246, 328, 448, 486, 1034
BstMWI GCNNNNNNNGC 6 cut(s) 369, 490, 527, 593, 602, 919
BstNI CCWGG 2 cut(s) 275, 1043
BstNSI RCATGY 1 cut(s) 421
BstSCI CCNGG 2 cut(s) 273, 1041
BstSFI CTRYAG 1 cut(s) 361
BstV1I GCAGC 2 cut(s) 900, 922
BstV2I GAAGAC 3 cut(s) 48, 855, 864
BstX2I RGATCY 2 cut(s) 246, 1034
BstYI RGATCY 2 cut(s) 246, 1034
BsuRI GGCC 3 cut(s) 5, 671, 1046
BtgI CCRYGG 1 cut(s) 282
BtsCI GGATG 5 cut(s) 43, 167, 451, 719, 1049
BtsI GCAGTG 1 cut(s) 379
BtsIMutI CAGTG 1 cut(s) 379
BveI ACCTGC 2 cut(s) 696, 823
Cac8I GCNNGC 2 cut(s) 230, 598
CaiI CAGNNNCTG 1 cut(s) 916
Cfr13I GGNCC 2 cut(s) 238, 468
Csp6I GTAC 4 cut(s) 198, 394, 658, 927
CviAII CATG 6 cut(s) 283, 418, 582, 661, 1063, 1078
CviQI GTAC 4 cut(s) 198, 394, 658, 927
DdeI CTNAG 3 cut(s) 352, 385, 759
DpnI GATC 6 cut(s) 27, 248, 330, 450, 488, 1036
DpnII GATC 6 cut(s) 25, 246, 328, 448, 486, 1034
EaeI YGGCCR 2 cut(s) 3, 669
Eam1104I CTCTTC 2 cut(s) 73, 191
EarI CTCTTC 2 cut(s) 73, 191
Ecl136II GAGCTC 3 cut(s) 66, 379, 954
Eco130I CCWWGG 1 cut(s) 282
Eco147I AGGCCT 1 cut(s) 1046
Eco24I GRGCYC 3 cut(s) 68, 381, 956
Eco47I GGWCC 2 cut(s) 238, 468
Eco53kI GAGCTC 3 cut(s) 66, 379, 954
Eco57I CTGAAG 2 cut(s) 500, 938
EcoICRI GAGCTC 3 cut(s) 66, 379, 954
EcoRII CCWGG 2 cut(s) 273, 1041
EcoT14I CCWWGG 1 cut(s) 282
EcoT38I GRGCYC 3 cut(s) 68, 381, 956
ErhI CCWWGG 1 cut(s) 282
FaeI CATG 6 cut(s) 286, 421, 585, 664, 1066, 1081
FalI AAGNNNNNCTT 1 cut(s) 33
FaqI GGGAC 2 cut(s) 526, 973
FatI CATG 6 cut(s) 282, 417, 581, 660, 1062, 1077
FbaI TGATCA 2 cut(s) 25, 448
Fnu4HI GCNGC 2 cut(s) 911, 914
FokI GGATG 5 cut(s) 50, 154, 438, 706, 1036
FriOI GRGCYC 3 cut(s) 68, 381, 956
Fsp4HI GCNGC 2 cut(s) 911, 914
GluI GCNGC 2 cut(s) 911, 914
GsaI CCCAGC 1 cut(s) 176
HaeIII GGCC 3 cut(s) 5, 671, 1046
Hin1II CATG 6 cut(s) 286, 421, 585, 664, 1066, 1081
HincII GTYRAC 1 cut(s) 900
HindII GTYRAC 1 cut(s) 900
HindIII AAGCTT 2 cut(s) 15, 292
HinfI GANTC 2 cut(s) 356, 693
HpaI GTTAAC 1 cut(s) 900
HphI GGTGA 5 cut(s) 35, 289, 299, 877, 1031
Hpy166II GTNNAC 3 cut(s) 471, 549, 900
Hpy188I TCNGA 5 cut(s) 355, 388, 403, 732, 943
Hpy188III TCNNGA 3 cut(s) 149, 1008, 1030
Hpy8I GTNNAC 3 cut(s) 471, 549, 900
HpyAV CCTTC 2 cut(s) 892, 956
HpyCH4III ACNGT 1 cut(s) 987
HpyF10VI GCNNNNNNNGC 6 cut(s) 369, 490, 527, 593, 602, 919
HpyF3I CTNAG 3 cut(s) 352, 385, 759
Hsp92II CATG 6 cut(s) 286, 421, 585, 664, 1066, 1081
Ksp22I TGATCA 2 cut(s) 25, 448
KspAI GTTAAC 1 cut(s) 900
Kzo9I GATC 6 cut(s) 25, 246, 328, 448, 486, 1034
LguI GCTCTTC 1 cut(s) 73
LmnI GCTCC 5 cut(s) 207, 317, 632, 653, 965
Lsp1109I GCAGC 2 cut(s) 900, 922
MaeIII GTNAC 2 cut(s) 440, 634
MalI GATC 6 cut(s) 27, 248, 330, 450, 488, 1036
MboI GATC 6 cut(s) 25, 246, 328, 448, 486, 1034
MboII GAAGA 8 cut(s) 53, 60, 178, 702, 837, 849, 855, 864
MfeI CAATTG 1 cut(s) 969
MflI RGATCY 2 cut(s) 246, 1034
MhlI GDGCHC 3 cut(s) 68, 381, 956
MlsI TGGCCA 2 cut(s) 5, 671
MluCI AATT 4 cut(s) 427, 937, 969, 1084
MluNI TGGCCA 2 cut(s) 5, 671
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 5 cut(s) 99, 285, 339, 675, 829
Mox20I TGGCCA 2 cut(s) 5, 671
MscI TGGCCA 2 cut(s) 5, 671
MseI TTAA 1 cut(s) 899
MslI CAYNNNNRTG 4 cut(s) 59, 422, 665, 731
Msp20I TGGCCA 2 cut(s) 5, 671
MspA1I CMGCKG 3 cut(s) 176, 913, 997
MspR9I CCNGG 2 cut(s) 275, 1043
MunI CAATTG 1 cut(s) 969
Mva1269I GAATGC 1 cut(s) 1017
MvaI CCWGG 2 cut(s) 275, 1043
MwoI GCNNNNNNNGC 6 cut(s) 369, 490, 527, 593, 602, 919
NcoI CCATGG 1 cut(s) 282
NdeII GATC 6 cut(s) 25, 246, 328, 448, 486, 1034
NlaIII CATG 6 cut(s) 286, 421, 585, 664, 1066, 1081
NlaIV GGNNCC 2 cut(s) 628, 710
NmuCI GTSAC 2 cut(s) 440, 634
NspI RCATGY 1 cut(s) 421
PaqCI CACCTGC 1 cut(s) 823
PceI AGGCCT 1 cut(s) 1046
PciI ACATGT 1 cut(s) 417
PciSI GCTCTTC 1 cut(s) 73
PcsI WCGNNNNNNNCGW 1 cut(s) 866
PctI GAATGC 1 cut(s) 1017
PfeI GAWTC 1 cut(s) 693
PflMI CCANNNNNTGG 1 cut(s) 997
PkrI GCNGC 2 cut(s) 912, 915
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
PscI ACATGT 1 cut(s) 417
Psp124BI GAGCTC 3 cut(s) 68, 381, 956
Psp6I CCWGG 2 cut(s) 273, 1041
PspFI CCCAGC 1 cut(s) 172
PspGI CCWGG 2 cut(s) 273, 1041
PspN4I GGNNCC 2 cut(s) 628, 710
PspPI GGNCC 2 cut(s) 238, 468
PstI CTGCAG 1 cut(s) 365
PstNI CAGNNNCTG 1 cut(s) 916
PsuI RGATCY 2 cut(s) 246, 1034
PvuII CAGCTG 3 cut(s) 176, 913, 997
RsaI GTAC 4 cut(s) 199, 395, 659, 928
RsaNI GTAC 4 cut(s) 198, 394, 658, 927
RseI CAYNNNNRTG 4 cut(s) 59, 422, 665, 731
SacI GAGCTC 3 cut(s) 68, 381, 956
SapI GCTCTTC 1 cut(s) 73
SaqAI TTAA 1 cut(s) 899
SatI GCNGC 2 cut(s) 911, 914
Sau3AI GATC 6 cut(s) 25, 246, 328, 448, 486, 1034
Sau96I GGNCC 2 cut(s) 238, 468
SbfI CCTGCAGG 1 cut(s) 365
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 2 cut(s) 275, 1043
SdaI CCTGCAGG 1 cut(s) 365
SduI GDGCHC 3 cut(s) 68, 381, 956
SfcI CTRYAG 1 cut(s) 361
SinI GGWCC 2 cut(s) 238, 468
SmiMI CAYNNNNRTG 4 cut(s) 59, 422, 665, 731
SmlI CTYRAG 4 cut(s) 147, 515, 889, 947
SmoI CTYRAG 4 cut(s) 147, 515, 889, 947
Sse8387I CCTGCAGG 1 cut(s) 365
Sse9I AATT 4 cut(s) 427, 937, 969, 1084
SseBI AGGCCT 1 cut(s) 1046
SsiI CCGC 1 cut(s) 35
SstI GAGCTC 3 cut(s) 68, 381, 956
StuI AGGCCT 1 cut(s) 1046
StyD4I CCNGG 2 cut(s) 273, 1041
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 1 cut(s) 987
TaqI TCGA 2 cut(s) 696, 956
TasI AATT 4 cut(s) 427, 937, 969, 1084
TatI WGTACW 2 cut(s) 393, 657
TfiI GAWTC 1 cut(s) 693
Tru1I TTAA 1 cut(s) 899
Tru9I TTAA 1 cut(s) 899
TscAI CASTG 1 cut(s) 379
TseFI GTSAC 2 cut(s) 440, 634
TseI GCWGC 2 cut(s) 910, 913
Tsp45I GTSAC 2 cut(s) 440, 634
TspDTI ATGAA 6 cut(s) 54, 195, 421, 677, 749, 1094
TspRI CASTG 1 cut(s) 379
Van91I CCANNNNNTGG 1 cut(s) 997
VpaK11BI GGWCC 2 cut(s) 238, 468
XapI RAATTY 2 cut(s) 427, 937
XceI RCATGY 1 cut(s) 421
XcmI CCANNNNNNNNNTGG 2 cut(s) 322, 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.