Rh3DG161000

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0015652
Physical Location & Seq
Reverse (-)
13959263 .. 13961647
2385 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG161000.1

Sequence Viewer

Length: 726 bp
ATGGCCAGAAACCAAGCTTCAAGTGATCATCAAACGTCAGATGAAGACTACATAGACATGGAAGTAAGTTCTTCTTCCTCAAACTTCTTCTGTTATTCCATTAGCTCTAGTCCACCACAAACCAGAGAGTTTGAGTTCCAAATGTCCTCAGTTCCTGACCATGACCAGCAGTCCACTACTTCTCCAGCTGATGAGCTCTTCTACAAAGGAACACTCCTCCCTCTTCATCTCCCACCTCGCTTGCAAATGGTCCAAAGTATCCTCCAAACCTCAAACAGTACCAAACTTGATACCAAAACCCAACAAGCTATTGAACAAAGCTTCAACCTTCCCTTCATCAAAAGCTCTAGAACCGCTCCCAACACTAACACAAGTACCCCATTGGATAGGTCCTGCAATATCTCACCCTCAGAGTCTTGCAGAGTAAGTAGTGAGCTAAACCCCGATGATTATGTCTCTGAATGGACAGAAGAAATGAGTGTTTTCATTGGTGATGATCCTTCAAAAAAATCTAATTGGTCAAAAAAGCTGAAGCAAATAAAGCAGTTCATTTTGAGTCAAAAGCTCAAGGCTTCAAGGGCATATATTAAGTCCTTGTTCAGTAAGTCCACTTGTGCAGATCATGAGTACTCCTCAGCCAAGTCAGCAAGCAATGCAGAACCAGAAAATGGCTCAAAAGGACTAACTAAGAAAATTGATGTAGAATACTCATTTGTAGAGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

241

Amino Acids

27.03

Weight (kDa)

5.43

Isoelectric Point (pI)

59.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015652)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g14700
malus_domestica MD04G1117200.v1.1 MD12G1132800.v1.1
prunus_persica Prupe.6G246800_v2.0.a1
pyrus_communis pycom04g10690
rosa_chinensis RchiOBHm_Chr3g0467911
rosa_laevigata RLG00000024468
rosa_multiflora Rmu_sc0001793.1_g000046 Rmu_sc0001793.1_g000047
rosa_roxburghii Rroxscaffold_6G00413320
rosa_rugosa Rorug03G0090500
rosa_samantha Rh3AG138800 Rh3BG160400 Rh3CG160400 Rh3DG161000
rosa_wichuraiana Rw3G013150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 668
AccBSI CCGCTC 1 cut(s) 356
AciI CCGC 1 cut(s) 354
AclWI GGATC 1 cut(s) 491
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 551
AfaI GTAC 3 cut(s) 280, 376, 629
AfiI CCNNNNNNNGG 1 cut(s) 668
AgsI TTSAA 5 cut(s) 21, 314, 325, 504, 576
AhdI GACNNNNNGTC 1 cut(s) 169
Alw21I GWGCWC 2 cut(s) 198, 723
Alw26I GTCTC 1 cut(s) 460
AlwI GGATC 1 cut(s) 491
AlwNI CAGNNNCTG 1 cut(s) 155
AoxI GGCC 1 cut(s) 3
AspS9I GGNCC 2 cut(s) 250, 390
AsuHPI GGTGA 2 cut(s) 396, 503
AvaII GGWCC 2 cut(s) 250, 390
BaeI ACNNNNGTAYC 2 cut(s) 358, 391
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 198
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 2 cut(s) 198, 723
BbvCI CCTCAGC 1 cut(s) 634
BciVI GTATCC 1 cut(s) 269
BclI TGATCA 1 cut(s) 25
BcoDI GTCTC 1 cut(s) 460
BfaI CTAG 3 cut(s) 108, 348, 724
BfuI GTATCC 1 cut(s) 269
BmcAI AGTACT 1 cut(s) 629
Bme18I GGWCC 2 cut(s) 250, 390
BmeRI GACNNNNNGTC 1 cut(s) 169
BmgT120I GGNCC 2 cut(s) 250, 390
BpiI GAAGAC 1 cut(s) 51
BplI GAGNNNNNCTC 2 cut(s) 617, 649
BpmI CTGGAG 1 cut(s) 168
Bpu10I CCTNAGC 1 cut(s) 634
BpuEI CTTGAG 1 cut(s) 551
BsaXI ACNNNNNCTCC 2 cut(s) 166, 196
Bsc4I CCNNNNNNNGG 1 cut(s) 668
Bse3DI GCAATG 1 cut(s) 658
BseLI CCNNNNNNNGG 1 cut(s) 668
BseMI GCAATG 1 cut(s) 658
BseMII CTCAG 3 cut(s) 162, 423, 648
BseRI GAGGAG 2 cut(s) 206, 622
BsgI GTGCAG 1 cut(s) 636
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 2 cut(s) 198, 723
BslI CCNNNNNNNGG 1 cut(s) 668
BsmAI GTCTC 1 cut(s) 460
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 2 cut(s) 198, 723
Bsp143I GATC 3 cut(s) 25, 496, 619
BspACI CCGC 1 cut(s) 354
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 161, 422, 647
BspHI TCATGA 1 cut(s) 622
BspPI GGATC 1 cut(s) 491
BspQI GCTCTTC 1 cut(s) 203
BsrBI CCGCTC 1 cut(s) 356
BsrDI GCAATG 1 cut(s) 658
BssMI GATC 3 cut(s) 25, 496, 619
Bst4CI ACNGT 1 cut(s) 278
Bst6I CTCTTC 2 cut(s) 203, 228
BstAPI GCANNNNNTGC 1 cut(s) 653
BstC8I GCNNGC 2 cut(s) 242, 649
BstDEI CTNAG 4 cut(s) 148, 409, 634, 687
BstKTI GATC 3 cut(s) 28, 499, 622
BstMAI GTCTC 1 cut(s) 460
BstMBI GATC 3 cut(s) 25, 496, 619
BstMWI GCNNNNNNNGC 4 cut(s) 541, 578, 644, 653
BstV2I GAAGAC 1 cut(s) 51
BsuI GTATCC 1 cut(s) 269
BsuRI GGCC 1 cut(s) 5
Cac8I GCNNGC 2 cut(s) 242, 649
CaiI CAGNNNCTG 1 cut(s) 155
CciI TCATGA 1 cut(s) 622
Cfr13I GGNCC 2 cut(s) 250, 390
Csp6I GTAC 3 cut(s) 279, 375, 628
CspCI CAANNNNNGTGG 2 cut(s) 222, 257
CviAII CATG 3 cut(s) 58, 161, 623
CviQI GTAC 3 cut(s) 279, 375, 628
DdeI CTNAG 4 cut(s) 148, 409, 634, 687
DpnI GATC 3 cut(s) 27, 498, 621
DpnII GATC 3 cut(s) 25, 496, 619
DriI GACNNNNNGTC 1 cut(s) 169
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 203, 228
Eam1105I GACNNNNNGTC 1 cut(s) 169
EarI CTCTTC 2 cut(s) 203, 228
Ecl136II GAGCTC 1 cut(s) 196
Eco24I GRGCYC 1 cut(s) 198
Eco47I GGWCC 2 cut(s) 250, 390
Eco53kI GAGCTC 1 cut(s) 196
Eco57I CTGAAG 1 cut(s) 551
EcoICRI GAGCTC 1 cut(s) 196
EcoO109I RGGNCCY 1 cut(s) 390
EcoT38I GRGCYC 1 cut(s) 198
FaeI CATG 3 cut(s) 61, 164, 626
FaiI YATR 7 cut(s) 53, 59, 162, 453, 583, 585, 624
FalI AAGNNNNNCTT 2 cut(s) 58, 90
FatI CATG 3 cut(s) 57, 160, 622
FbaI TGATCA 1 cut(s) 25
FriOI GRGCYC 1 cut(s) 198
FspBI CTAG 3 cut(s) 108, 348, 724
GsuI CTGGAG 1 cut(s) 168
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 3 cut(s) 61, 164, 626
HindIII AAGCTT 2 cut(s) 15, 319
HinfI GANTC 2 cut(s) 413, 556
HphI GGTGA 2 cut(s) 396, 503
Hpy166II GTNNAC 3 cut(s) 113, 174, 609
Hpy188I TCNGA 3 cut(s) 40, 412, 460
Hpy188III TCNNGA 3 cut(s) 155, 348, 623
Hpy8I GTNNAC 3 cut(s) 113, 174, 609
HpyAV CCTTC 3 cut(s) 338, 343, 510
HpyCH4III ACNGT 1 cut(s) 278
HpyCH4IV ACGT 1 cut(s) 35
HpyCH4V TGCA 5 cut(s) 244, 396, 420, 617, 656
HpyF10VI GCNNNNNNNGC 4 cut(s) 541, 578, 644, 653
HpyF3I CTNAG 4 cut(s) 148, 409, 634, 687
HpySE526I ACGT 1 cut(s) 35
Hsp92II CATG 3 cut(s) 61, 164, 626
Ksp22I TGATCA 1 cut(s) 25
Kzo9I GATC 3 cut(s) 25, 496, 619
LguI GCTCTTC 1 cut(s) 203
LmnI GCTCC 1 cut(s) 361
LpnPI CCDG 7 cut(s) 19, 136, 168, 179, 198, 406, 675
MaeI CTAG 3 cut(s) 108, 348, 724
MaeII ACGT 1 cut(s) 35
MalI GATC 3 cut(s) 27, 498, 621
MbiI CCGCTC 1 cut(s) 356
MboI GATC 3 cut(s) 25, 496, 619
MboII GAAGA 7 cut(s) 56, 63, 66, 79, 190, 215, 482
MhlI GDGCHC 2 cut(s) 198, 723
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 514, 693
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 422, 565
MnlI CCTC 9 cut(s) 88, 157, 227, 231, 246, 272, 280, 418, 643
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 588
MslI CAYNNNNRTG 1 cut(s) 56
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 188
MwoI GCNNNNNNNGC 4 cut(s) 541, 578, 644, 653
NdeII GATC 3 cut(s) 25, 496, 619
NlaIII CATG 3 cut(s) 61, 164, 626
PagI TCATGA 1 cut(s) 622
PciSI GCTCTTC 1 cut(s) 203
PflMI CCANNNNNTGG 1 cut(s) 668
PleI GAGTC 2 cut(s) 421, 564
PpsI GAGTC 2 cut(s) 421, 564
PpuMI RGGWCCY 1 cut(s) 390
Psp124BI GAGCTC 1 cut(s) 198
Psp5II RGGWCCY 1 cut(s) 390
PspPI GGNCC 2 cut(s) 250, 390
PspPPI RGGWCCY 1 cut(s) 390
PstNI CAGNNNCTG 1 cut(s) 155
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 3 cut(s) 280, 376, 629
RsaNI GTAC 3 cut(s) 279, 375, 628
RseI CAYNNNNRTG 1 cut(s) 56
SacI GAGCTC 1 cut(s) 198
SapI GCTCTTC 1 cut(s) 203
SaqAI TTAA 1 cut(s) 588
Sau3AI GATC 3 cut(s) 25, 496, 619
Sau96I GGNCC 2 cut(s) 250, 390
ScaI AGTACT 1 cut(s) 629
SchI GAGTC 2 cut(s) 422, 565
SduI GDGCHC 2 cut(s) 198, 723
SinI GGWCC 2 cut(s) 250, 390
SmiMI CAYNNNNRTG 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
Sse9I AATT 2 cut(s) 514, 693
SsiI CCGC 1 cut(s) 354
SspMI CTAG 3 cut(s) 108, 348, 724
SstI GAGCTC 1 cut(s) 198
TaaI ACNGT 1 cut(s) 278
TaiI ACGT 1 cut(s) 38
TasI AATT 2 cut(s) 514, 693
TatI WGTACW 1 cut(s) 627
Tru1I TTAA 1 cut(s) 588
Tru9I TTAA 1 cut(s) 588
TspDTI ATGAA 5 cut(s) 57, 215, 325, 475, 538
Van91I CCANNNNNTGG 1 cut(s) 668
VpaK11BI GGWCC 2 cut(s) 250, 390
XbaI TCTAGA 1 cut(s) 347
XspI CTAG 3 cut(s) 108, 348, 724
ZrmI AGTACT 1 cut(s) 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.