pycom04g10690

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0015652
Physical Location & Seq
Forward (+)
13446825 .. 13447913
1089 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g10690.1

Sequence Viewer

Length: 1089 bp
ATGGCCAGAAGCCAAGCTCCAGGTGATCATAGTTCAGATGAAGACTACATAGACATGGAAGTGAGCTCTTCCTCCAACTTCTTGTGCTATTCCATCAGCTCTCCACCACAAACCAGAGAGTTTGAGTTCCAGATGTCCTCACGTTGTCAAGACAAAGAAACCACAAATTCCCCAGCTGATGACCTCTTCTACAAAGGTAAGCTCCTCCCACTCCACCTCCCACCTCGCCTGCAAATGGTCCAAAAGATCCTCCAAACCTCCAACAACAAAACACAGGAAGCCATTGACGAAAGCTTCAGCAAAAGGGAAAGCTCCAGTATCCCATTGGACCAGTCCTACAATATCGCCCCCTCAGAGTCCTGCAGGGTCAGCAGTGAACTCAACTCAGATGAGTACATCTCCGAATGGACGGCTGACATGTGTGTGAATTTCATTGGTGGGCACAATGATCATCAAAAGAAATCTTGGTCTACAAAGCTAAAGCAGATCAAGCAGTCCTCATTGGGTCAAAAGCTCAAGGCTTCAAGGGCATATGTGAAGTCCCTGTTCAGAAAAACCACCTGTGCGGATGACTACAACTCATGTGCCGAAGCAGAAGCAGAAAGTGGCTCAAAAGTCAGAGACTTTTGCTCCAACAAGTACATGAAAGTGGCTGAGAAAACCCCATTTGGAAGAGTCGACGGTGGCAGATTCCAAATATCATCAACCACTCTCATGAAAAGCATTGGGAACGAAATGGCCGAGGAAAACTCAAACACCCACAGAAAATCTTTCTCTGGGGCAATTCAACATACAACTGCCCTGCAATCTCTGTGTTCACCTACATCTTCCTCCGTTTCTTCGTCTTCCTCGTCTTCTTTCTCCTTCAGCTCAAGTGGATTCTCAGATTTGCAGCTGCTGAAGCGAAGTACGAGTGCAAATTCAGAGCTTGAGAGCTCAATCGAGGGGGCGATTGCTCATTGCAAGCAGTCCCAGCAGCTGTTGAGTTCAAGAATGCAGTTTGTGGGGGTGAAGAGATCTCACCAAGGTTCATCCAATAATCCTATGAGAGACGCACACATGAATAATTTGATTGCATTATATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

363

Amino Acids

40.09

Weight (kDa)

6.76

Isoelectric Point (pI)

60.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015652)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g14700
malus_domestica MD04G1117200.v1.1 MD12G1132800.v1.1
prunus_persica Prupe.6G246800_v2.0.a1
pyrus_communis pycom04g10690
rosa_chinensis RchiOBHm_Chr3g0467911
rosa_laevigata RLG00000024468
rosa_multiflora Rmu_sc0001793.1_g000046 Rmu_sc0001793.1_g000047
rosa_roxburghii Rroxscaffold_6G00413320
rosa_rugosa Rorug03G0090500
rosa_samantha Rh3AG138800 Rh3BG160400 Rh3CG160400 Rh3DG161000
rosa_wichuraiana Rw3G013150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 470, 678
AciI CCGC 1 cut(s) 566
AclWI GGATC 1 cut(s) 241
AcoI YGGCCR 2 cut(s) 3, 738
AcsI RAATTY 3 cut(s) 166, 427, 919
AcuI CTGAAG 3 cut(s) 280, 850, 920
AfaI GTAC 3 cut(s) 395, 641, 910
AfiI CCNNNNNNNGG 1 cut(s) 235
AflIII ACRYGT 1 cut(s) 417
AgsI TTSAA 3 cut(s) 525, 788, 990
AjnI CCWGG 1 cut(s) 19
Alw21I GWGCWC 2 cut(s) 68, 938
Alw26I GTCTC 2 cut(s) 615, 1044
AlwI GGATC 1 cut(s) 241
AlwNI CAGNNNCTG 2 cut(s) 898, 979
AoxI GGCC 2 cut(s) 3, 738
ApeKI GCWGC 3 cut(s) 892, 895, 976
ApoI RAATTY 3 cut(s) 166, 427, 919
AspS9I GGNCC 2 cut(s) 238, 328
AsuHPI GGTGA 4 cut(s) 35, 810, 1013, 1021
AvaII GGWCC 2 cut(s) 238, 328
BaeGI GKGCMC 1 cut(s) 444
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 2 cut(s) 68, 938
BbsI GAAGAC 3 cut(s) 48, 837, 846
Bbv12I GWGCWC 2 cut(s) 68, 938
BbvI GCAGC 3 cut(s) 882, 904, 988
BccI CCATC 1 cut(s) 101
BceAI ACGGC 1 cut(s) 426
BciT130I CCWGG 1 cut(s) 21
BciVI GTATCC 1 cut(s) 329
BclI TGATCA 2 cut(s) 25, 448
BcoDI GTCTC 2 cut(s) 615, 1044
BfmI CTRYAG 1 cut(s) 361
BfuI GTATCC 1 cut(s) 329
BglII AGATCT 1 cut(s) 1016
BisI GCNGC 3 cut(s) 893, 896, 977
BlsI GCNGC 3 cut(s) 894, 897, 978
Bme1390I CCNGG 1 cut(s) 21
Bme18I GGWCC 2 cut(s) 238, 328
BmgT120I GGNCC 2 cut(s) 238, 328
BmrFI CCNGG 1 cut(s) 21
BpiI GAAGAC 3 cut(s) 48, 837, 846
BplI GAGNNNNNCTC 4 cut(s) 383, 415, 734, 766
BpmI CTGGAG 1 cut(s) 298
BpuEI CTTGAG 3 cut(s) 500, 856, 950
BsaJI CCNNGG 2 cut(s) 741, 1024
BsaXI ACNNNNNCTCC 4 cut(s) 201, 231, 614, 644
Bsc4I CCNNNNNNNGG 1 cut(s) 235
Bse1I ACTGG 2 cut(s) 315, 331
Bse3DI GCAATG 1 cut(s) 958
BseBI CCWGG 1 cut(s) 21
BseDI CCNNGG 2 cut(s) 741, 1024
BseGI GGATG 2 cut(s) 574, 1031
BseLI CCNNNNNNNGG 1 cut(s) 235
BseMI GCAATG 1 cut(s) 958
BseMII CTCAG 4 cut(s) 366, 399, 645, 897
BseNI ACTGG 2 cut(s) 315, 331
BseRI GAGGAG 1 cut(s) 194
BseSI GKGCMC 1 cut(s) 444
BseXI GCAGC 3 cut(s) 882, 904, 988
BseYI CCCAGC 2 cut(s) 172, 972
BshFI GGCC 2 cut(s) 5, 740
BsiHKAI GWGCWC 2 cut(s) 68, 938
BslFI GGGAC 2 cut(s) 526, 955
BslI CCNNNNNNNGG 1 cut(s) 235
BsmAI GTCTC 2 cut(s) 615, 1044
BsmBI CGTCTC 1 cut(s) 1044
BsmFI GGGAC 2 cut(s) 526, 955
BsmI GAATGC 1 cut(s) 999
BsnI GGCC 2 cut(s) 5, 740
Bsp1286I GDGCHC 3 cut(s) 68, 444, 938
Bsp143I GATC 5 cut(s) 25, 246, 448, 486, 1016
BspACI CCGC 1 cut(s) 566
BspANI GGCC 2 cut(s) 5, 740
BspCNI CTCAG 4 cut(s) 365, 398, 646, 896
BspHI TCATGA 1 cut(s) 714
BspMAI CTGCAG 1 cut(s) 365
BspPI GGATC 1 cut(s) 241
BspQI GCTCTTC 1 cut(s) 73
BsrDI GCAATG 1 cut(s) 958
BsrI ACTGG 2 cut(s) 315, 331
BssECI CCNNGG 2 cut(s) 741, 1024
BssMI GATC 5 cut(s) 25, 246, 448, 486, 1016
BssT1I CCWWGG 1 cut(s) 1024
Bst2UI CCWGG 1 cut(s) 21
Bst4CI ACNGT 1 cut(s) 683
Bst6I CTCTTC 4 cut(s) 73, 191, 667, 1007
BstC8I GCNNGC 2 cut(s) 230, 965
BstDEI CTNAG 4 cut(s) 352, 385, 654, 883
BstF5I GGATG 2 cut(s) 574, 1031
BstKTI GATC 5 cut(s) 28, 249, 451, 489, 1019
BstMAI GTCTC 2 cut(s) 615, 1044
BstMBI GATC 5 cut(s) 25, 246, 448, 486, 1016
BstMWI GCNNNNNNNGC 5 cut(s) 369, 490, 527, 901, 973
BstNI CCWGG 1 cut(s) 21
BstNSI RCATGY 1 cut(s) 421
BstSCI CCNGG 1 cut(s) 19
BstSFI CTRYAG 1 cut(s) 361
BstSLI GKGCMC 1 cut(s) 444
BstV1I GCAGC 3 cut(s) 882, 904, 988
BstV2I GAAGAC 3 cut(s) 48, 837, 846
BstX2I RGATCY 2 cut(s) 246, 1016
BstYI RGATCY 2 cut(s) 246, 1016
BsuI GTATCC 1 cut(s) 329
BsuRI GGCC 2 cut(s) 5, 740
BtsCI GGATG 2 cut(s) 574, 1031
BtsI GCAGTG 1 cut(s) 379
BtsIMutI CAGTG 1 cut(s) 379
Cac8I GCNNGC 2 cut(s) 230, 965
CaiI CAGNNNCTG 2 cut(s) 898, 979
CciI TCATGA 1 cut(s) 714
Cfr13I GGNCC 2 cut(s) 238, 328
CseI GACGC 1 cut(s) 1061
Csp6I GTAC 3 cut(s) 394, 640, 909
CviAII CATG 6 cut(s) 55, 418, 582, 643, 715, 1060
CviQI GTAC 3 cut(s) 394, 640, 909
DdeI CTNAG 4 cut(s) 352, 385, 654, 883
DpnI GATC 5 cut(s) 27, 248, 450, 488, 1018
DpnII GATC 5 cut(s) 25, 246, 448, 486, 1016
EaeI YGGCCR 2 cut(s) 3, 738
Eam1104I CTCTTC 4 cut(s) 73, 191, 667, 1007
EarI CTCTTC 4 cut(s) 73, 191, 667, 1007
Ecl136II GAGCTC 2 cut(s) 66, 936
Eco130I CCWWGG 1 cut(s) 1024
Eco24I GRGCYC 2 cut(s) 68, 938
Eco47I GGWCC 2 cut(s) 238, 328
Eco53kI GAGCTC 2 cut(s) 66, 936
Eco57I CTGAAG 3 cut(s) 280, 850, 920
EcoICRI GAGCTC 2 cut(s) 66, 936
EcoRII CCWGG 1 cut(s) 19
EcoT14I CCWWGG 1 cut(s) 1024
EcoT38I GRGCYC 2 cut(s) 68, 938
ErhI CCWWGG 1 cut(s) 1024
Esp3I CGTCTC 1 cut(s) 1044
FaeI CATG 6 cut(s) 58, 421, 585, 646, 718, 1063
FaqI GGGAC 2 cut(s) 526, 955
FatI CATG 6 cut(s) 54, 417, 581, 642, 714, 1059
FauNDI CATATG 1 cut(s) 532
FbaI TGATCA 2 cut(s) 25, 448
FblI GTMKAC 2 cut(s) 470, 678
Fnu4HI GCNGC 3 cut(s) 893, 896, 977
FokI GGATG 2 cut(s) 581, 1018
FriOI GRGCYC 2 cut(s) 68, 938
Fsp4HI GCNGC 3 cut(s) 893, 896, 977
GluI GCNGC 3 cut(s) 893, 896, 977
GsaI CCCAGC 2 cut(s) 176, 976
GsuI CTGGAG 1 cut(s) 298
HaeIII GGCC 2 cut(s) 5, 740
HgaI GACGC 1 cut(s) 1061
Hin1II CATG 6 cut(s) 58, 421, 585, 646, 718, 1063
HincII GTYRAC 1 cut(s) 679
HindII GTYRAC 1 cut(s) 679
HindIII AAGCTT 1 cut(s) 292
HinfI GANTC 4 cut(s) 356, 675, 690, 879
HphI GGTGA 4 cut(s) 35, 810, 1013, 1021
Hpy166II GTNNAC 4 cut(s) 377, 471, 679, 818
Hpy188I TCNGA 8 cut(s) 37, 355, 388, 403, 551, 620, 886, 925
Hpy188III TCNNGA 4 cut(s) 130, 149, 715, 990
Hpy8I GTNNAC 4 cut(s) 377, 471, 679, 818
Hpy99I CGWCG 1 cut(s) 683
HpyAV CCTTC 1 cut(s) 874
HpyCH4III ACNGT 1 cut(s) 683
HpyCH4IV ACGT 1 cut(s) 142
HpyCH4V TGCA 8 cut(s) 232, 363, 805, 892, 917, 963, 997, 1076
HpyF10VI GCNNNNNNNGC 5 cut(s) 369, 490, 527, 901, 973
HpyF3I CTNAG 4 cut(s) 352, 385, 654, 883
HpySE526I ACGT 1 cut(s) 142
Hsp92II CATG 6 cut(s) 58, 421, 585, 646, 718, 1063
Ksp22I TGATCA 2 cut(s) 25, 448
Kzo9I GATC 5 cut(s) 25, 246, 448, 486, 1016
LguI GCTCTTC 1 cut(s) 73
LmnI GCTCC 4 cut(s) 22, 207, 317, 635
Lsp1109I GCAGC 3 cut(s) 882, 904, 988
MaeII ACGT 1 cut(s) 142
MalI GATC 5 cut(s) 27, 248, 450, 488, 1018
MboI GATC 5 cut(s) 25, 246, 448, 486, 1016
MboII GAAGA 9 cut(s) 53, 60, 178, 684, 819, 831, 837, 846, 1024
MflI RGATCY 2 cut(s) 246, 1016
MhlI GDGCHC 3 cut(s) 68, 444, 938
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 166, 427, 783, 919, 1066
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 365, 684
MmeI TCCRAC 3 cut(s) 99, 285, 657
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 5 cut(s) 53, 59, 422, 647, 713
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 176, 895, 979
MspR9I CCNGG 1 cut(s) 21
Mva1269I GAATGC 1 cut(s) 999
MvaI CCWGG 1 cut(s) 21
MwoI GCNNNNNNNGC 5 cut(s) 369, 490, 527, 901, 973
NdeI CATATG 1 cut(s) 532
NdeII GATC 5 cut(s) 25, 246, 448, 486, 1016
NlaIII CATG 6 cut(s) 58, 421, 585, 646, 718, 1063
NmeAIII GCCGAG 1 cut(s) 766
NspI RCATGY 1 cut(s) 421
PagI TCATGA 1 cut(s) 714
PciI ACATGT 1 cut(s) 417
PciSI GCTCTTC 1 cut(s) 73
PcsI WCGNNNNNNNCGW 2 cut(s) 738, 848
PctI GAATGC 1 cut(s) 999
PfeI GAWTC 2 cut(s) 690, 879
PkrI GCNGC 3 cut(s) 894, 897, 978
PleI GAGTC 2 cut(s) 364, 683
PpsI GAGTC 2 cut(s) 364, 683
PscI ACATGT 1 cut(s) 417
Psp124BI GAGCTC 2 cut(s) 68, 938
Psp6I CCWGG 1 cut(s) 19
PspFI CCCAGC 2 cut(s) 172, 972
PspGI CCWGG 1 cut(s) 19
PspPI GGNCC 2 cut(s) 238, 328
PstI CTGCAG 1 cut(s) 365
PstNI CAGNNNCTG 2 cut(s) 898, 979
PsuI RGATCY 2 cut(s) 246, 1016
PvuII CAGCTG 3 cut(s) 176, 895, 979
RsaI GTAC 3 cut(s) 395, 641, 910
RsaNI GTAC 3 cut(s) 394, 640, 909
RseI CAYNNNNRTG 5 cut(s) 53, 59, 422, 647, 713
SacI GAGCTC 2 cut(s) 68, 938
SalI GTCGAC 1 cut(s) 677
SapI GCTCTTC 1 cut(s) 73
SatI GCNGC 3 cut(s) 893, 896, 977
Sau3AI GATC 5 cut(s) 25, 246, 448, 486, 1016
Sau96I GGNCC 2 cut(s) 238, 328
SbfI CCTGCAGG 1 cut(s) 365
SchI GAGTC 2 cut(s) 365, 684
ScrFI CCNGG 1 cut(s) 21
SdaI CCTGCAGG 1 cut(s) 365
SduI GDGCHC 3 cut(s) 68, 444, 938
SfcI CTRYAG 1 cut(s) 361
SinI GGWCC 2 cut(s) 238, 328
SmiMI CAYNNNNRTG 5 cut(s) 53, 59, 422, 647, 713
SmlI CTYRAG 3 cut(s) 515, 871, 929
SmoI CTYRAG 3 cut(s) 515, 871, 929
Sse8387I CCTGCAGG 1 cut(s) 365
Sse9I AATT 5 cut(s) 166, 427, 783, 919, 1066
SsiI CCGC 1 cut(s) 566
SstI GAGCTC 2 cut(s) 68, 938
StyD4I CCNGG 1 cut(s) 19
StyI CCWWGG 1 cut(s) 1024
TaaI ACNGT 1 cut(s) 683
TaiI ACGT 1 cut(s) 145
TaqI TCGA 2 cut(s) 678, 942
TasI AATT 5 cut(s) 166, 427, 783, 919, 1066
TatI WGTACW 2 cut(s) 393, 639
TfiI GAWTC 2 cut(s) 690, 879
TscAI CASTG 1 cut(s) 379
TseI GCWGC 3 cut(s) 892, 895, 976
TspDTI ATGAA 6 cut(s) 54, 421, 659, 731, 1020, 1076
TspGWI ACGGA 1 cut(s) 823
TspRI CASTG 1 cut(s) 379
VpaK11BI GGWCC 2 cut(s) 238, 328
XapI RAATTY 3 cut(s) 166, 427, 919
XceI RCATGY 1 cut(s) 421
XcmI CCANNNNNNNNNTGG 1 cut(s) 322
XmiI GTMKAC 2 cut(s) 470, 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.