MD04G1123500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016545
Physical Location & Seq
Forward (+)
20912804 .. 20913166
363 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1123500.v1.1.491

Sequence Viewer

Length: 363 bp
ATGCTCCGTTCACTCTTCACGTCCACCCTCACGCGCCTCTCCTCTCGCTGGCCAGTGCTAATCTACGCCGCCTCGTGGACCACAGTTCTCATTCTGACGGTGGCCGTGGCCTCCTTCTCGCCCGAGGTTGCTTTCGTGTCCGCCATTTCGTCGACGTCTAAGTTCTCCCGCGTCTGCGGCTCAGAAGGGTCGATCAGGATTCCATCGGACGTGCCGGGAGACGTCCTGTGCCTGCCGGCTCACCTGTTCAGCAAGTCCGCCATCGATTACATCGTGCCTCCTGTTTTCGCGGCAGCCGTGGTGGCTGCGTCGGCTTACCTGGTTAGAGCCGTGGGCTTGTGGGAGGCCGATGAGGTCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

121

Amino Acids

12.75

Weight (kDa)

6.54

Isoelectric Point (pI)

50.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016545)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37530
fragaria_vesca FvH4_6g14180
malus_domestica MD04G1123500.v1.1 MD12G1139400.v1.1
prunus_persica Prupe.6G251200_v2.0.a1
pyrus_communis pycom04g11140
rosa_chinensis RchiOBHm_Chr3g0467161
rosa_laevigata RLG00000024523
rosa_multiflora Rmu_sc0000962.1_g000079 Rmu_sc0018989.1_g000004
rosa_roxburghii Rroxscaffold_6G00413860
rosa_rugosa Rorug03G0085500
rosa_samantha Rh3BG155000
rosa_wichuraiana Rw3G012650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 158, 225
AccI GTMKAC 1 cut(s) 152
AccII CGCG 3 cut(s) 34, 171, 290
AciI CCGC 6 cut(s) 69, 141, 169, 177, 258, 290
AcoI YGGCCR 2 cut(s) 50, 102
AcyI GRCGYC 2 cut(s) 155, 222
AfiI CCNNNNNNNGG 2 cut(s) 48, 75
AjiI CACGTC 2 cut(s) 21, 211
AjnI CCWGG 1 cut(s) 318
Alw26I GTCTC 1 cut(s) 213
Ama87I CYCGRG 1 cut(s) 122
AoxI GGCC 4 cut(s) 50, 102, 108, 345
ApeKI GCWGC 2 cut(s) 293, 305
AspLEI GCGC 1 cut(s) 36
AspS9I GGNCC 2 cut(s) 78, 355
AsuC2I CCSGG 1 cut(s) 216
AsuHPI GGTGA 1 cut(s) 233
AvaI CYCGRG 1 cut(s) 122
AvaII GGWCC 2 cut(s) 78, 355
BalI TGGCCA 1 cut(s) 52
BauI CACGAG 1 cut(s) 73
BbvI GCAGC 2 cut(s) 292, 305
BccI CCATC 2 cut(s) 211, 269
BceAI ACGGC 3 cut(s) 89, 281, 314
BciT130I CCWGG 1 cut(s) 320
BcnI CCSGG 1 cut(s) 216
BcoDI GTCTC 1 cut(s) 213
BglI GCCNNNNNGGC 1 cut(s) 302
BisI GCNGC 5 cut(s) 69, 178, 291, 294, 306
BlsI GCNGC 5 cut(s) 70, 179, 292, 295, 307
Bme1390I CCNGG 2 cut(s) 216, 320
Bme18I GGWCC 2 cut(s) 78, 355
BmeT110I CYCGRG 1 cut(s) 122
BmgBI CACGTC 2 cut(s) 21, 211
BmgT120I GGNCC 2 cut(s) 78, 355
BmiI GGNNCC 1 cut(s) 357
BmrFI CCNGG 2 cut(s) 216, 320
BpuMI CCSGG 1 cut(s) 216
Bsa29I ATCGAT 1 cut(s) 264
BsaHI GRCGYC 2 cut(s) 155, 222
BsaJI CCNNGG 4 cut(s) 105, 123, 297, 330
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 75
Bse118I RCCGGY 1 cut(s) 235
Bse1I ACTGG 1 cut(s) 53
BseBI CCWGG 1 cut(s) 320
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 4 cut(s) 105, 123, 297, 330
BseLI CCNNNNNNNGG 2 cut(s) 48, 75
BseMII CTCAG 1 cut(s) 195
BseNI ACTGG 1 cut(s) 53
BseRI GAGGAG 1 cut(s) 31
BseXI GCAGC 2 cut(s) 292, 305
Bsh1236I CGCG 3 cut(s) 34, 171, 290
BshFI GGCC 4 cut(s) 52, 104, 110, 347
BshVI ATCGAT 1 cut(s) 264
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 2 cut(s) 215, 236
BslFI GGGAC 1 cut(s) 341
BslI CCNNNNNNNGG 2 cut(s) 48, 75
BsmAI GTCTC 1 cut(s) 213
BsmBI CGTCTC 1 cut(s) 213
BsmFI GGGAC 1 cut(s) 341
BsnI GGCC 4 cut(s) 52, 104, 110, 347
BsoBI CYCGRG 1 cut(s) 122
Bsp143I GATC 1 cut(s) 192
BspACI CCGC 6 cut(s) 69, 141, 169, 177, 258, 290
BspANI GGCC 4 cut(s) 52, 104, 110, 347
BspCNI CTCAG 1 cut(s) 194
BspDI ATCGAT 1 cut(s) 264
BspFNI CGCG 3 cut(s) 34, 171, 290
BspLI GGNNCC 1 cut(s) 357
BsrFI RCCGGY 1 cut(s) 235
BsrI ACTGG 1 cut(s) 53
BssAI RCCGGY 1 cut(s) 235
BssECI CCNNGG 4 cut(s) 105, 123, 297, 330
BssMI GATC 1 cut(s) 192
BssNI GRCGYC 2 cut(s) 155, 222
BssSI CACGAG 1 cut(s) 73
Bst2BI CACGAG 1 cut(s) 73
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 2 cut(s) 85, 100
Bst6I CTCTTC 1 cut(s) 20
BstACI GRCGYC 2 cut(s) 155, 222
BstC8I GCNNGC 3 cut(s) 50, 233, 237
BstDEI CTNAG 2 cut(s) 159, 181
BstDSI CCRYGG 3 cut(s) 105, 297, 330
BstFNI CGCG 3 cut(s) 34, 171, 290
BstHHI GCGC 1 cut(s) 36
BstKTI GATC 1 cut(s) 195
BstMAI GTCTC 1 cut(s) 213
BstMBI GATC 1 cut(s) 192
BstMWI GCNNNNNNNGC 3 cut(s) 177, 302, 311
BstNI CCWGG 1 cut(s) 320
BstSCI CCNGG 2 cut(s) 214, 318
BstUI CGCG 3 cut(s) 34, 171, 290
BstV1I GCAGC 2 cut(s) 292, 305
Bsu15I ATCGAT 1 cut(s) 264
BsuRI GGCC 4 cut(s) 52, 104, 110, 347
BsuTUI ATCGAT 1 cut(s) 264
BtgI CCRYGG 3 cut(s) 105, 297, 330
BtrI CACGTC 2 cut(s) 21, 211
BtsIMutI CAGTG 1 cut(s) 60
Cac8I GCNNGC 3 cut(s) 50, 233, 237
CfoI GCGC 1 cut(s) 36
Cfr10I RCCGGY 1 cut(s) 235
Cfr13I GGNCC 2 cut(s) 78, 355
ClaI ATCGAT 1 cut(s) 264
CseI GACGC 2 cut(s) 160, 297
CsiI ACCWGGT 1 cut(s) 318
DdeI CTNAG 2 cut(s) 159, 181
DpnI GATC 1 cut(s) 194
DpnII GATC 1 cut(s) 192
EaeI YGGCCR 2 cut(s) 50, 102
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
EciI GGCGGA 2 cut(s) 130, 247
Eco47I GGWCC 2 cut(s) 78, 355
Eco88I CYCGRG 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 355
EcoRII CCWGG 1 cut(s) 318
Esp3I CGTCTC 1 cut(s) 213
FaqI GGGAC 1 cut(s) 341
FauI CCCGC 1 cut(s) 176
FblI GTMKAC 1 cut(s) 152
Fnu4HI GCNGC 5 cut(s) 69, 178, 291, 294, 306
Fsp4HI GCNGC 5 cut(s) 69, 178, 291, 294, 306
GlaI GCGC 1 cut(s) 35
GluI GCNGC 5 cut(s) 69, 178, 291, 294, 306
HaeIII GGCC 4 cut(s) 52, 104, 110, 347
HapII CCGG 2 cut(s) 215, 236
HgaI GACGC 2 cut(s) 160, 297
HhaI GCGC 1 cut(s) 36
Hin1I GRCGYC 2 cut(s) 155, 222
Hin6I GCGC 1 cut(s) 34
HinP1I GCGC 1 cut(s) 34
HincII GTYRAC 1 cut(s) 153
HindII GTYRAC 1 cut(s) 153
HinfI GANTC 1 cut(s) 199
HpaII CCGG 2 cut(s) 215, 236
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 4 cut(s) 11, 24, 78, 153
Hpy188I TCNGA 3 cut(s) 96, 184, 208
Hpy188III TCNNGA 1 cut(s) 196
Hpy8I GTNNAC 4 cut(s) 11, 24, 78, 153
Hpy99I CGWCG 3 cut(s) 154, 157, 313
HpyAV CCTTC 2 cut(s) 124, 179
HpyCH4III ACNGT 2 cut(s) 85, 100
HpyCH4IV ACGT 4 cut(s) 20, 155, 210, 222
HpyF10VI GCNNNNNNNGC 3 cut(s) 177, 302, 311
HpyF3I CTNAG 2 cut(s) 159, 181
HpySE526I ACGT 4 cut(s) 20, 155, 210, 222
Hsp92I GRCGYC 2 cut(s) 155, 222
HspAI GCGC 1 cut(s) 34
KroI GCCGGC 1 cut(s) 235
KroNI GCCGGC 1 cut(s) 237
Kzo9I GATC 1 cut(s) 192
LmnI GCTCC 1 cut(s) 9
Lsp1109I GCAGC 2 cut(s) 292, 305
MabI ACCWGGT 1 cut(s) 318
MaeII ACGT 4 cut(s) 20, 155, 210, 222
MalI GATC 1 cut(s) 194
MboI GATC 1 cut(s) 192
MboII GAAGA 1 cut(s) 7
MlsI TGGCCA 1 cut(s) 52
MluNI TGGCCA 1 cut(s) 52
MnlI CCTC 9 cut(s) 38, 47, 52, 82, 118, 121, 288, 337, 346
Mox20I TGGCCA 1 cut(s) 52
MroNI GCCGGC 1 cut(s) 235
MscI TGGCCA 1 cut(s) 52
Msp20I TGGCCA 1 cut(s) 52
MspI CCGG 2 cut(s) 215, 236
MspR9I CCNGG 2 cut(s) 216, 320
MvaI CCWGG 1 cut(s) 320
MvnI CGCG 3 cut(s) 34, 171, 290
MwoI GCNNNNNNNGC 3 cut(s) 177, 302, 311
NaeI GCCGGC 1 cut(s) 237
NciI CCSGG 1 cut(s) 216
NdeII GATC 1 cut(s) 192
NgoMIV GCCGGC 1 cut(s) 235
NlaIV GGNNCC 1 cut(s) 357
PcsI WCGNNNNNNNCGW 2 cut(s) 270, 294
PdiI GCCGGC 1 cut(s) 237
PfeI GAWTC 1 cut(s) 199
PkrI GCNGC 5 cut(s) 70, 179, 292, 295, 307
PpuMI RGGWCCY 1 cut(s) 355
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 1 cut(s) 318
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 1 cut(s) 357
PspPI GGNCC 2 cut(s) 78, 355
PspPPI RGGWCCY 1 cut(s) 355
SalI GTCGAC 1 cut(s) 151
SatI GCNGC 5 cut(s) 69, 178, 291, 294, 306
Sau3AI GATC 1 cut(s) 192
Sau96I GGNCC 2 cut(s) 78, 355
ScrFI CCNGG 2 cut(s) 216, 320
SetI ASST 8 cut(s) 23, 129, 158, 213, 225, 246, 321, 357
SexAI ACCWGGT 1 cut(s) 318
SgrDI CGTCGACG 1 cut(s) 151
SinI GGWCC 2 cut(s) 78, 355
SsiI CCGC 6 cut(s) 69, 141, 169, 177, 258, 290
StyD4I CCNGG 2 cut(s) 214, 318
TaaI ACNGT 2 cut(s) 85, 100
TaiI ACGT 4 cut(s) 23, 158, 213, 225
TaqI TCGA 3 cut(s) 152, 191, 264
TauI GCSGC 3 cut(s) 71, 180, 293
TfiI GAWTC 1 cut(s) 199
TscAI CASTG 1 cut(s) 60
TseI GCWGC 2 cut(s) 293, 305
TspRI CASTG 1 cut(s) 60
VpaK11BI GGWCC 2 cut(s) 78, 355
XmiI GTMKAC 1 cut(s) 152
ZraI GACGTC 2 cut(s) 156, 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.