MD12G1139400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016545
Physical Location & Seq
Forward (+)
21706639 .. 21707058
420 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1139400.v1.1.491

Sequence Viewer

Length: 420 bp
ATGCATATAAATTCCCAACCTCACTACTCCTCTCCCCCAAAAACCCCAATCAACACAATGCTCCGCTCCCTCCTCACCTCCACCGTCACGCGCCTCTCCCCGCGCTGGCCAGTGCTTATCTACGCCGTCACGTGGACCACATTGCTCACTCTGACAGTGGCTGTGGCCTCTTTCTCTCCCGAGGTTGCCTTCGTGTCCGCCATTTCGTCGACGTCTAAGTTCTCCCTAGTCTGCGGCTCGGAAGGGTCGATTAGGATTCCGTCAGACGTGCCGGGAGACATCCTATGCCTGCCGGCTCACCTGTTCAGCAAGTCCGCCATCGATTTCATCGTGCCTCCTGTTTTCGCGGCGGTCGTGGTGGCTGCCTCGGCTTACCTGATTAGAGCTGTAGGTTTGTGGGAGGCAGATGAGGTCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

140

Amino Acids

14.92

Weight (kDa)

6.89

Isoelectric Point (pI)

57.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016545)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37530
fragaria_vesca FvH4_6g14180
malus_domestica MD04G1123500.v1.1 MD12G1139400.v1.1
prunus_persica Prupe.6G251200_v2.0.a1
pyrus_communis pycom04g11140
rosa_chinensis RchiOBHm_Chr3g0467161
rosa_laevigata RLG00000024523
rosa_multiflora Rmu_sc0000962.1_g000079 Rmu_sc0018989.1_g000004
rosa_roxburghii Rroxscaffold_6G00413860
rosa_rugosa Rorug03G0085500
rosa_samantha Rh3BG155000
rosa_wichuraiana Rw3G012650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 215
AccBSI CCGCTC 1 cut(s) 66
AccI GTMKAC 1 cut(s) 209
AccII CGCG 3 cut(s) 91, 103, 347
AciI CCGC 7 cut(s) 64, 101, 198, 234, 315, 347, 350
AcoI YGGCCR 1 cut(s) 107
AcsI RAATTY 1 cut(s) 10
AcvI CACGTG 1 cut(s) 132
AcyI GRCGYC 1 cut(s) 212
AfiI CCNNNNNNNGG 2 cut(s) 105, 132
AjiI CACGTC 1 cut(s) 268
AluBI AGCT 1 cut(s) 386
AluI AGCT 1 cut(s) 386
Alw26I GTCTC 1 cut(s) 270
AlwNI CAGNNNCTG 1 cut(s) 161
Ama87I CYCGRG 1 cut(s) 179
AoxI GGCC 2 cut(s) 107, 165
ApeKI GCWGC 1 cut(s) 362
ApoI RAATTY 1 cut(s) 10
AspLEI GCGC 2 cut(s) 93, 105
AspS9I GGNCC 2 cut(s) 135, 412
AsuC2I CCSGG 1 cut(s) 273
AsuHPI GGTGA 2 cut(s) 67, 290
AvaI CYCGRG 1 cut(s) 179
AvaII GGWCC 2 cut(s) 135, 412
BalI TGGCCA 1 cut(s) 109
BbrPI CACGTG 1 cut(s) 132
BbvI GCAGC 1 cut(s) 349
BccI CCATC 1 cut(s) 326
BceAI ACGGC 1 cut(s) 110
BcnI CCSGG 1 cut(s) 273
BcoDI GTCTC 1 cut(s) 270
BfaI CTAG 1 cut(s) 227
BfmI CTRYAG 1 cut(s) 387
BisI GCNGC 3 cut(s) 235, 348, 363
BlsI GCNGC 3 cut(s) 236, 349, 364
Bme1390I CCNGG 1 cut(s) 273
Bme18I GGWCC 2 cut(s) 135, 412
BmeT110I CYCGRG 1 cut(s) 179
BmgBI CACGTC 1 cut(s) 268
BmgT120I GGNCC 2 cut(s) 135, 412
BmrFI CCNGG 1 cut(s) 273
BpuMI CCSGG 1 cut(s) 273
Bsa29I ATCGAT 1 cut(s) 321
BsaAI YACGTR 1 cut(s) 132
BsaHI GRCGYC 1 cut(s) 212
BsaJI CCNNGG 2 cut(s) 180, 366
Bsc4I CCNNNNNNNGG 2 cut(s) 105, 132
Bse118I RCCGGY 1 cut(s) 292
Bse1I ACTGG 1 cut(s) 110
Bse3DI GCAATG 1 cut(s) 140
BseCI ATCGAT 1 cut(s) 321
BseDI CCNNGG 2 cut(s) 180, 366
BseGI GGATG 1 cut(s) 279
BseLI CCNNNNNNNGG 2 cut(s) 105, 132
BseMI GCAATG 1 cut(s) 140
BseNI ACTGG 1 cut(s) 110
BseRI GAGGAG 2 cut(s) 19, 62
BseXI GCAGC 1 cut(s) 349
Bsh1236I CGCG 3 cut(s) 91, 103, 347
Bsh1285I CGRYCG 1 cut(s) 354
BshFI GGCC 2 cut(s) 109, 167
BshVI ATCGAT 1 cut(s) 321
BsiEI CGRYCG 1 cut(s) 354
BsiHKCI CYCGRG 1 cut(s) 179
BsiSI CCGG 2 cut(s) 272, 293
BslI CCNNNNNNNGG 2 cut(s) 105, 132
BsmAI GTCTC 1 cut(s) 270
BsnI GGCC 2 cut(s) 109, 167
BsoBI CYCGRG 1 cut(s) 179
BspACI CCGC 7 cut(s) 64, 101, 198, 234, 315, 347, 350
BspANI GGCC 2 cut(s) 109, 167
BspDI ATCGAT 1 cut(s) 321
BspFNI CGCG 3 cut(s) 91, 103, 347
BsrBI CCGCTC 1 cut(s) 66
BsrDI GCAATG 1 cut(s) 140
BsrFI RCCGGY 1 cut(s) 292
BsrI ACTGG 1 cut(s) 110
BssAI RCCGGY 1 cut(s) 292
BssECI CCNNGG 2 cut(s) 180, 366
BssNI GRCGYC 1 cut(s) 212
Bst4CI ACNGT 2 cut(s) 85, 157
BstACI GRCGYC 1 cut(s) 212
BstBAI YACGTR 1 cut(s) 132
BstC8I GCNNGC 3 cut(s) 107, 290, 294
BstDEI CTNAG 1 cut(s) 216
BstF5I GGATG 1 cut(s) 279
BstFNI CGCG 3 cut(s) 91, 103, 347
BstHHI GCGC 2 cut(s) 93, 105
BstMAI GTCTC 1 cut(s) 270
BstMCI CGRYCG 1 cut(s) 354
BstMWI GCNNNNNNNGC 1 cut(s) 368
BstSCI CCNGG 1 cut(s) 271
BstSFI CTRYAG 1 cut(s) 387
BstUI CGCG 3 cut(s) 91, 103, 347
BstV1I GCAGC 1 cut(s) 349
Bsu15I ATCGAT 1 cut(s) 321
BsuRI GGCC 2 cut(s) 109, 167
BsuTUI ATCGAT 1 cut(s) 321
BtrI CACGTC 1 cut(s) 268
BtsCI GGATG 1 cut(s) 279
BtsIMutI CAGTG 2 cut(s) 117, 162
Cac8I GCNNGC 3 cut(s) 107, 290, 294
CaiI CAGNNNCTG 1 cut(s) 161
CfoI GCGC 2 cut(s) 93, 105
Cfr10I RCCGGY 1 cut(s) 292
Cfr13I GGNCC 2 cut(s) 135, 412
ClaI ATCGAT 1 cut(s) 321
CviJI RGCY 8 cut(s) 109, 161, 167, 237, 296, 362, 371, 386
CviKI_1 RGCY 8 cut(s) 109, 161, 167, 237, 296, 362, 371, 386
DdeI CTNAG 1 cut(s) 216
EaeI YGGCCR 1 cut(s) 107
EciI GGCGGA 2 cut(s) 187, 304
Eco47I GGWCC 2 cut(s) 135, 412
Eco72I CACGTG 1 cut(s) 132
Eco88I CYCGRG 1 cut(s) 179
EcoT22I ATGCAT 1 cut(s) 6
FaiI YATR 3 cut(s) 6, 8, 286
FauI CCCGC 1 cut(s) 108
FblI GTMKAC 1 cut(s) 209
Fnu4HI GCNGC 3 cut(s) 235, 348, 363
FokI GGATG 1 cut(s) 266
Fsp4HI GCNGC 3 cut(s) 235, 348, 363
FspBI CTAG 1 cut(s) 227
GlaI GCGC 2 cut(s) 92, 104
GluI GCNGC 3 cut(s) 235, 348, 363
HaeIII GGCC 2 cut(s) 109, 167
HapII CCGG 2 cut(s) 272, 293
HhaI GCGC 2 cut(s) 93, 105
Hin1I GRCGYC 1 cut(s) 212
Hin6I GCGC 2 cut(s) 91, 103
HinP1I GCGC 2 cut(s) 91, 103
HincII GTYRAC 1 cut(s) 210
HindII GTYRAC 1 cut(s) 210
HinfI GANTC 1 cut(s) 256
HpaII CCGG 2 cut(s) 272, 293
HphI GGTGA 2 cut(s) 67, 290
Hpy166II GTNNAC 2 cut(s) 135, 210
Hpy188I TCNGA 3 cut(s) 153, 241, 265
Hpy188III TCNNGA 1 cut(s) 179
Hpy8I GTNNAC 2 cut(s) 135, 210
Hpy99I CGWCG 2 cut(s) 211, 214
HpyAV CCTTC 2 cut(s) 199, 236
HpyCH4III ACNGT 2 cut(s) 85, 157
HpyCH4IV ACGT 3 cut(s) 131, 212, 267
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 368
HpyF3I CTNAG 1 cut(s) 216
HpySE526I ACGT 3 cut(s) 131, 212, 267
Hsp92I GRCGYC 1 cut(s) 212
HspAI GCGC 2 cut(s) 91, 103
KroI GCCGGC 1 cut(s) 292
KroNI GCCGGC 1 cut(s) 294
LmnI GCTCC 2 cut(s) 66, 71
LpnPI CCDG 8 cut(s) 91, 123, 285, 302, 306, 314, 351, 389
Lsp1109I GCAGC 1 cut(s) 349
MaeI CTAG 1 cut(s) 227
MaeII ACGT 3 cut(s) 131, 212, 267
MaeIII GTNAC 2 cut(s) 85, 127
MbiI CCGCTC 1 cut(s) 66
MlsI TGGCCA 1 cut(s) 109
MluCI AATT 1 cut(s) 10
MluNI TGGCCA 1 cut(s) 109
Mox20I TGGCCA 1 cut(s) 109
Mph1103I ATGCAT 1 cut(s) 6
MroNI GCCGGC 1 cut(s) 292
MscI TGGCCA 1 cut(s) 109
Msp20I TGGCCA 1 cut(s) 109
MspI CCGG 2 cut(s) 272, 293
MspR9I CCNGG 1 cut(s) 273
MvnI CGCG 3 cut(s) 91, 103, 347
MwoI GCNNNNNNNGC 1 cut(s) 368
NaeI GCCGGC 1 cut(s) 294
NciI CCSGG 1 cut(s) 273
NgoMIV GCCGGC 1 cut(s) 292
NmeAIII GCCGAG 1 cut(s) 347
NmuCI GTSAC 2 cut(s) 85, 127
NsiI ATGCAT 1 cut(s) 6
PcsI WCGNNNNNNNCGW 3 cut(s) 245, 327, 351
PdiI GCCGGC 1 cut(s) 294
PfeI GAWTC 1 cut(s) 256
PkrI GCNGC 3 cut(s) 236, 349, 364
PmaCI CACGTG 1 cut(s) 132
PmlI CACGTG 1 cut(s) 132
Ppu21I YACGTR 1 cut(s) 132
PspCI CACGTG 1 cut(s) 132
PspPI GGNCC 2 cut(s) 135, 412
PstNI CAGNNNCTG 1 cut(s) 161
SalI GTCGAC 1 cut(s) 208
SatI GCNGC 3 cut(s) 235, 348, 363
Sau96I GGNCC 2 cut(s) 135, 412
ScrFI CCNGG 1 cut(s) 273
SfcI CTRYAG 1 cut(s) 387
SgrDI CGTCGACG 1 cut(s) 208
SinI GGWCC 2 cut(s) 135, 412
Sse9I AATT 1 cut(s) 10
SsiI CCGC 7 cut(s) 64, 101, 198, 234, 315, 347, 350
SspMI CTAG 1 cut(s) 227
StyD4I CCNGG 1 cut(s) 271
TaaI ACNGT 2 cut(s) 85, 157
TaiI ACGT 3 cut(s) 134, 215, 270
TaqI TCGA 3 cut(s) 209, 248, 321
TasI AATT 1 cut(s) 10
TauI GCSGC 2 cut(s) 237, 350
TfiI GAWTC 1 cut(s) 256
TscAI CASTG 2 cut(s) 117, 162
TseFI GTSAC 2 cut(s) 85, 127
TseI GCWGC 1 cut(s) 362
Tsp45I GTSAC 2 cut(s) 85, 127
TspDTI ATGAA 1 cut(s) 316
TspGWI ACGGA 1 cut(s) 249
TspRI CASTG 2 cut(s) 117, 162
VpaK11BI GGWCC 2 cut(s) 135, 412
XapI RAATTY 1 cut(s) 10
XmiI GTMKAC 1 cut(s) 209
XspI CTAG 1 cut(s) 227
ZraI GACGTC 1 cut(s) 213
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.