Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0010990

Physical Location & Seq

Genomic Coordinates Forward (+)

23496907 .. 23506056

Gene Structure 9150 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1146600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2052 bp

ATGATTGCTGTCATGGACTTGAATGCCTCGCCAGTACCTGAAGAAGATGAAGATATTTTTGAGAATCATATAGAATACATTGCACCAGAAGAACGCATAGAGAGTGGAGCTGATATAGCACGCCGGGAGCGTGAAGAAAGACGTAAGCGATTGAAACGAGAACGCCCAGATGAAAAACCAGTGCATGTTTCTCAACCCCCTGCATACGATTCATATCAAACAAAAAACCCCAAAGTTTACGACAAATCTAGGCTTCCCAATGGTTGGTTGGATTGCCCGGCAGCTGGTCAAGAAATATCTTGCATTATACCTTCTAAGGTGCCACTTGGTGAATCCTACAATGATTGTGTTCCTCCTGGTAAAAGATACTCATTTAAACAAGTGATTCATCAGCAAAGAGTTTTGGGAAGAAAACTTGGTTTGGTGATTGATTTGACAAATACTTCTCGCTATTATCTAACATCAGATTTGAAGAAAGAGGGTATTAAGCATGTCAAGATTGCGTGCAAGGGGCGGGATTCCGTGCCGGACAACCTTTCTGTAAATCAGTTTGTATACGAGGTTATACAGTTCCTGTCACGTCAAAAGCATTCGAAGAAATATATTCTTGTGCATTGTACACATGGGCACAATCGCACGGGGTATATGATCATCCACTACCTAATGCGCTCATTACCAATTTCCGTCACTCAGGCAATAAAAATGTTTGCAGATGCACGTCCTCCAGGAATCTACAAACCTGACTATGTTGATGCTTTATACTCTTTCTATCATGAAAAAAAGCCTGATATGGTTGTCTGTCCCTCAACTCCTGAATGGAAGACTTCAGAATTTGATCTTAATGGCGAAGCAGTTGCAGACGACGATGACGATGATGGAGGTCCTGCTGCTCCTGTAAATAATGAGAGTCTGGATACAAGTGGAGTAATGACAAATGATGATATCTTGGGCGATGAGATACCTTGGGACCAGCAGGAGGCAATGCGGCAGTTCTGCTATCAAACTCTTAAGCTGGTTAATGTTGGGCCTAGAGGAAATTCACAATTCCCGGGGTCGCACCCAGTTTCTCTTGACAGGGACAACTTACAGCTGTTGAGGCAACGTTATTATTATGCTACATGGAAGGCTGATGGAACACGATATATGATGTTAATAACCATGGATGGGTGTTACTTGGTTGATAGAAATTTTAATTTTAGAAGGGTCCAAATGAGGTTTCCCTGCAAACACACAAATGATGGTTTAGCCGAGAAGACTCACCACTACACTTTATTGGATGGGGAGATGGTAATTGATACCGTGCCTGATTCAAAGAAGCAGGAGAGAAGATACCTGATCTATGATATGATGGCAATCAACCACGTTCCTATCATAGAGCGGCCTTTTTATGAACGGTGGAAGATGCTTGAGAAAGAAGTCATTGAACCTCGGAATCATGAGCGCCACAATATTTACCAAAGTAGGAATCCTTACTATAGATATGACTTGGAACCATTCAGGGTGAGGAGGAAGGACTTTTGGTTGCTCTCTACAGTAAACAAGCTTTTGAAGGAATTTATTCCTAGGCTTTCACACGATGCTGATGGTCTTATCTTTCAGGGTTGGGATGATCCATATGTTCCCCGCACACATGCAGGACTTCTAAAGTGGAAATATTCTCATATGAACTCGGTTGATTTTCTCTTTGAGGTAACCGATGATGATCGGCAGCATCTTTTTCTGTTTGAACGTGGAAAGAAGAAACTAATGGACGGAAATAGAGTTGCGTTTAAAGATGGCCAAGACCCCTCATCTTATTCCGGAAAAATAATCGAGTGTTCTTGGGATGGTGAAGAACAAGAATGGGTCCTTATGCGCATCAGGACTGACAAATCAACTCCAAATGATATCAATACCTACAAGAAGGTCATGCGAAGCATAAAAGACAATATCACGCAGGACATCTTGTTGAATGAGATCTATGAGATCATTCGTCTGCCTATGTATGCTGATAGGATACGGAATGATAGCAAAGCAGCCCAGCAGCATGCAAATGCAGCACGAAGGAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004484 GO:0004651 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006370 GO:0006396 GO:0006397 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008192 GO:0009452 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010604 GO:0010628 GO:0016070 GO:0016071 GO:0016311 GO:0016462 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016791 GO:0016817 GO:0016818 GO:0019222 GO:0034641 GO:0036260 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048518 GO:0050355 GO:0050789 GO:0060255 GO:0065007 GO:0070568 GO:0071704 GO:0090304 GO:0098501 GO:0098507 GO:0098518 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

DSPc mRNA_cap_C mRNA_cap_enzyme

Protein Analysis

684

Amino Acids

80.02

Weight (kDa)

6.9

Isoelectric Point (pI)

49.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DSPc	PF00782	137 - 257	1.6e-15	Dual specificity phosphatase, catalytic domain
mRNA_cap_enzyme	PF01331	353 - 551	3.6e-76	mRNA capping enzyme, catalytic domain
mRNA_cap_C	PF03919	556 - 650	5.2e-20	mRNA capping enzyme, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010990)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09100 AT3G09100
fragaria_vesca	FvH4_6g12120 FvH4_6g12120 FvH4_6g12120
malus_domestica	MD04G1146600.v1.1 MD12G1159800.v1.1
prunus_persica	Prupe.6G270400_v2.0.a1
pyrus_communis	pycom04g13230 pycom12g15160
rosa_chinensis	RchiOBHm_Chr3g0463921
rosa_laevigata	RLG00000024736
rosa_multiflora	Rmu_sc0000517.1_g000001
rosa_roxburghii	Rroxscaffold_6G00416140
rosa_rugosa	Rorug03G0069200
rosa_samantha	Rh3AG126200 Rh3BG130200 Rh3CG132700 Rh3DG131400
rosa_wichuraiana	Rw3G010650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1856
AccB1I	GGYRCC	1 cut(s)	319
AccB7I	CCANNNNNTGG	1 cut(s)	264
AccBSI	CCGCTC	1 cut(s)	1378
AccI	GTMKAC	1 cut(s)	555
AccIII	TCCGGA	1 cut(s)	1799
AciI	CCGC	4 cut(s)	514, 985, 1378, 1624
AclI	AACGTT	1 cut(s)	1102
AclWI	GGATC	1 cut(s)	1604
AcoI	YGGCCR	1 cut(s)	1777
AcsI	RAATTY	4 cut(s)	830, 1036, 1186, 1553
AcuI	CTGAAG	2 cut(s)	60, 810
AdeI	CACNNNGTG	1 cut(s)	329
AfaI	GTAC	2 cut(s)	36, 619
AfiI	CCNNNNNNNGG	4 cut(s)	264, 284, 976, 1164
AflII	CTTAAG	1 cut(s)	1007
AgsI	TTSAA	8 cut(s)	22, 154, 472, 1311, 1424, 1549, 1727, 1951
AjiI	CACGTC	2 cut(s)	581, 719
AjnI	CCWGG	2 cut(s)	355, 724
AjuI	GAANNNNNNNTTGG	2 cut(s)	1200, 1232
AluBI	AGCT	5 cut(s)	110, 284, 1012, 1090, 1543
AluI	AGCT	5 cut(s)	110, 284, 1012, 1090, 1543
AlwI	GGATC	1 cut(s)	1604
AlwNI	CAGNNNCTG	2 cut(s)	38, 574
Ama87I	CYCGRG	1 cut(s)	1048
Aor13HI	TCCGGA	1 cut(s)	1799
AoxI	GGCC	3 cut(s)	1025, 1379, 1777
ApeKI	GCWGC	6 cut(s)	281, 887, 1708, 2015, 2023, 2036
ApoI	RAATTY	4 cut(s)	830, 1036, 1186, 1553
Asp700I	GAANNNNTTC	1 cut(s)	1557
AspA2I	CCTAGG	1 cut(s)	1562
AspLEI	GCGC	3 cut(s)	669, 1443, 1857
AspS9I	GGNCC	5 cut(s)	881, 967, 1025, 1204, 1846
AsuC2I	CCSGG	4 cut(s)	125, 278, 1049, 1050
AsuHPI	GGTGA	5 cut(s)	341, 436, 1250, 1513, 1841
AsuII	TTCGAA	1 cut(s)	593
AvaI	CYCGRG	1 cut(s)	1048
AvaII	GGWCC	4 cut(s)	881, 967, 1204, 1846
AvrII	CCTAGG	1 cut(s)	1562
BaeGI	GKGCMC	1 cut(s)	630
BalI	TGGCCA	1 cut(s)	1779
BanI	GGYRCC	1 cut(s)	319
BbsI	GAAGAC	2 cut(s)	827, 1259
BbvI	GCAGC	6 cut(s)	293, 874, 1720, 2027, 2035, 2048
BcgI	CGANNNNNNTGC	2 cut(s)	836, 870
BciT130I	CCWGG	2 cut(s)	357, 726
BciVI	GTATCC	2 cut(s)	907, 1989
BclI	TGATCA	1 cut(s)	648
BcnI	CCSGG	4 cut(s)	125, 278, 1049, 1050
BfaI	CTAG	3 cut(s)	249, 1029, 1563
BfmI	CTRYAG	2 cut(s)	1474, 1530
BfoI	RGCGCY	1 cut(s)	1444
BfrI	CTTAAG	1 cut(s)	1007
BfuI	GTATCC	2 cut(s)	907, 1989
BglII	AGATCT	1 cut(s)	1956
BisI	GCNGC	8 cut(s)	282, 888, 986, 1379, 1709, 2016, 2024, 2037
BlnI	CCTAGG	1 cut(s)	1562
BlsI	GCNGC	8 cut(s)	283, 889, 987, 1380, 1710, 2017, 2025, 2038
Bme1390I	CCNGG	6 cut(s)	125, 278, 357, 726, 1049, 1050
Bme18I	GGWCC	4 cut(s)	881, 967, 1204, 1846
BmeT110I	CYCGRG	1 cut(s)	1048
BmgBI	CACGTC	2 cut(s)	581, 719
BmgT120I	GGNCC	5 cut(s)	881, 967, 1025, 1204, 1846
BmiI	GGNNCC	5 cut(s)	321, 968, 1205, 1491, 1847
BmrFI	CCNGG	6 cut(s)	125, 278, 357, 726, 1049, 1050
BmrI	ACTGGG	1 cut(s)	1055
BmsI	GCATC	6 cut(s)	703, 742, 1392, 1567, 1720, 1866
BmuI	ACTGGG	1 cut(s)	1055
BpiI	GAAGAC	2 cut(s)	827, 1259
BpmI	CTGGAG	1 cut(s)	708
Bpu14I	TTCGAA	1 cut(s)	593
BpuEI	CTTGAG	1 cut(s)	1427
BpuMI	CCSGG	4 cut(s)	125, 278, 1049, 1050
BsaBI	GATNNNNATC	3 cut(s)	213, 1352, 1701
BsaJI	CCNNGG	6 cut(s)	962, 1048, 1049, 1158, 1427, 1562
BsaWI	WCCGGW	1 cut(s)	1799
Bsc4I	CCNNNNNNNGG	4 cut(s)	264, 284, 976, 1164
Bse1I	ACTGG	3 cut(s)	32, 179, 1061
Bse3DI	GCAATG	2 cut(s)	78, 987
Bse8I	GATNNNNATC	3 cut(s)	213, 1352, 1701
BseAI	TCCGGA	1 cut(s)	1799
BseBI	CCWGG	2 cut(s)	357, 726
BseDI	CCNNGG	6 cut(s)	962, 1048, 1049, 1158, 1427, 1562
BseGI	GGATG	5 cut(s)	651, 1168, 1282, 1612, 1831
BseJI	GATNNNNATC	3 cut(s)	213, 1352, 1701
BseLI	CCNNNNNNNGG	4 cut(s)	264, 284, 976, 1164
BseMI	GCAATG	2 cut(s)	78, 987
BseMII	CTCAG	1 cut(s)	704
BseNI	ACTGG	3 cut(s)	32, 179, 1061
BseRI	GAGGAG	1 cut(s)	1519
BseSI	GKGCMC	1 cut(s)	630
BseXI	GCAGC	6 cut(s)	293, 874, 1720, 2027, 2035, 2048
BseYI	CCCAGC	1 cut(s)	2019
BshFI	GGCC	3 cut(s)	1027, 1381, 1779
BshNI	GGYRCC	1 cut(s)	319
BsiHKCI	CYCGRG	1 cut(s)	1048
BsiSI	CCGG	5 cut(s)	124, 278, 527, 1049, 1800
BslFI	GGGAC	3 cut(s)	786, 980, 1091
BslI	CCNNNNNNNGG	4 cut(s)	264, 284, 976, 1164
BsmFI	GGGAC	3 cut(s)	786, 980, 1091
BsmI	GAATGC	2 cut(s)	28, 589
BsnI	GGCC	3 cut(s)	1027, 1381, 1779
BsoBI	CYCGRG	1 cut(s)	1048
Bsp119I	TTCGAA	1 cut(s)	593
Bsp1286I	GDGCHC	1 cut(s)	630
Bsp13I	TCCGGA	1 cut(s)	1799
Bsp1407I	TGTACA	1 cut(s)	617
Bsp143I	GATC	7 cut(s)	648, 835, 1335, 1609, 1702, 1956, 1965
Bsp19I	CCATGG	1 cut(s)	1158
BspACI	CCGC	4 cut(s)	514, 985, 1378, 1624
BspANI	GGCC	3 cut(s)	1027, 1381, 1779
BspCNI	CTCAG	1 cut(s)	703
BspEI	TCCGGA	1 cut(s)	1799
BspHI	TCATGA	2 cut(s)	772, 1435
BspLI	GGNNCC	5 cut(s)	321, 968, 1205, 1491, 1847
BspPI	GGATC	1 cut(s)	1604
BspT104I	TTCGAA	1 cut(s)	593
BspT107I	GGYRCC	1 cut(s)	319
BspTI	CTTAAG	1 cut(s)	1007
BsrBI	CCGCTC	1 cut(s)	1378
BsrDI	GCAATG	2 cut(s)	78, 987
BsrGI	TGTACA	1 cut(s)	617
BsrI	ACTGG	3 cut(s)	32, 179, 1061
BssECI	CCNNGG	6 cut(s)	962, 1048, 1049, 1158, 1427, 1562
BssMI	GATC	7 cut(s)	648, 835, 1335, 1609, 1702, 1956, 1965
BssNAI	GTATAC	1 cut(s)	556
BssT1I	CCWWGG	3 cut(s)	962, 1158, 1562
Bst1107I	GTATAC	1 cut(s)	556
Bst2UI	CCWGG	2 cut(s)	357, 726
Bst4CI	ACNGT	4 cut(s)	570, 1300, 1395, 1534
BstAFI	CTTAAG	1 cut(s)	1007
BstAUI	TGTACA	1 cut(s)	617
BstBI	TTCGAA	1 cut(s)	593
BstC8I	GCNNGC	3 cut(s)	121, 505, 2028
BstDEI	CTNAG	2 cut(s)	315, 690
BstDSI	CCRYGG	1 cut(s)	1158
BstEII	GGTNACC	1 cut(s)	1690
BstF5I	GGATG	5 cut(s)	651, 1168, 1282, 1612, 1831
BstH2I	RGCGCY	1 cut(s)	1444
BstHHI	GCGC	3 cut(s)	669, 1443, 1857
BstKTI	GATC	7 cut(s)	651, 838, 1338, 1612, 1705, 1959, 1968
BstMBI	GATC	7 cut(s)	648, 835, 1335, 1609, 1702, 1956, 1965
BstMWI	GCNNNNNNNGC	3 cut(s)	116, 1096, 2036
BstNI	CCWGG	2 cut(s)	357, 726
BstNSI	RCATGY	4 cut(s)	188, 494, 1634, 2030
BstPI	GGTNACC	1 cut(s)	1690
BstSCI	CCNGG	6 cut(s)	123, 276, 355, 724, 1047, 1048
BstSFI	CTRYAG	2 cut(s)	1474, 1530
BstSLI	GKGCMC	1 cut(s)	630
BstV1I	GCAGC	6 cut(s)	293, 874, 1720, 2027, 2035, 2048
BstV2I	GAAGAC	2 cut(s)	827, 1259
BstX2I	RGATCY	1 cut(s)	1956
BstYI	RGATCY	1 cut(s)	1956
BstZ17I	GTATAC	1 cut(s)	556
BsuI	GTATCC	2 cut(s)	907, 1989
BsuRI	GGCC	3 cut(s)	1027, 1381, 1779
BtgI	CCRYGG	1 cut(s)	1158
BtgZI	GCGATG	1 cut(s)	966
BtrI	CACGTC	2 cut(s)	581, 719
BtsCI	GGATG	5 cut(s)	651, 1168, 1282, 1612, 1831
BtsIMutI	CAGTG	1 cut(s)	186
Cac8I	GCNNGC	3 cut(s)	121, 505, 2028
CaiI	CAGNNNCTG	2 cut(s)	38, 574
CciI	TCATGA	2 cut(s)	772, 1435
CfoI	GCGC	3 cut(s)	669, 1443, 1857
Cfr13I	GGNCC	5 cut(s)	881, 967, 1025, 1204, 1846
Cfr9I	CCCGGG	1 cut(s)	1048
Csp6I	GTAC	2 cut(s)	35, 618
CviQI	GTAC	2 cut(s)	35, 618
DdeI	CTNAG	2 cut(s)	315, 690
DpnI	GATC	7 cut(s)	650, 837, 1337, 1611, 1704, 1958, 1967
DpnII	GATC	7 cut(s)	648, 835, 1335, 1609, 1702, 1956, 1965
DraI	TTTAAA	2 cut(s)	376, 1771
DraIII	CACNNNGTG	1 cut(s)	329
EaeI	YGGCCR	1 cut(s)	1777
Eco130I	CCWWGG	3 cut(s)	962, 1158, 1562
Eco32I	GATATC	2 cut(s)	943, 1888
Eco47I	GGWCC	4 cut(s)	881, 967, 1204, 1846
Eco57I	CTGAAG	2 cut(s)	60, 810
Eco88I	CYCGRG	1 cut(s)	1048
Eco91I	GGTNACC	1 cut(s)	1690
EcoO109I	RGGNCCY	2 cut(s)	881, 1846
EcoO65I	GGTNACC	1 cut(s)	1690
EcoRII	CCWGG	2 cut(s)	355, 724
EcoRV	GATATC	2 cut(s)	943, 1888
EcoT14I	CCWWGG	3 cut(s)	962, 1158, 1562
ErhI	CCWWGG	3 cut(s)	962, 1158, 1562
FaqI	GGGAC	3 cut(s)	786, 980, 1091
FauI	CCCGC	2 cut(s)	507, 1631
FauNDI	CATATG	2 cut(s)	1615, 1662
FbaI	TGATCA	1 cut(s)	648
FblI	GTMKAC	1 cut(s)	555
Fnu4HI	GCNGC	8 cut(s)	282, 888, 986, 1379, 1709, 2016, 2024, 2037
FokI	GGATG	5 cut(s)	638, 1175, 1289, 1619, 1838
Fsp4HI	GCNGC	8 cut(s)	282, 888, 986, 1379, 1709, 2016, 2024, 2037
FspAI	RTGCGCAY	1 cut(s)	1856
FspBI	CTAG	3 cut(s)	249, 1029, 1563
FspI	TGCGCA	1 cut(s)	1856
GlaI	GCGC	3 cut(s)	668, 1442, 1856
GluI	GCNGC	8 cut(s)	282, 888, 986, 1379, 1709, 2016, 2024, 2037
GsaI	CCCAGC	1 cut(s)	2023
GsuI	CTGGAG	1 cut(s)	708
HaeII	RGCGCY	1 cut(s)	1444
HaeIII	GGCC	3 cut(s)	1027, 1381, 1779
HapII	CCGG	5 cut(s)	124, 278, 527, 1049, 1800
HhaI	GCGC	3 cut(s)	669, 1443, 1857
Hin6I	GCGC	3 cut(s)	667, 1441, 1855
HinP1I	GCGC	3 cut(s)	667, 1441, 1855
HindIII	AAGCTT	1 cut(s)	1541
HpaII	CCGG	5 cut(s)	124, 278, 527, 1049, 1800
HphI	GGTGA	5 cut(s)	341, 436, 1250, 1513, 1841
Hpy166II	GTNNAC	4 cut(s)	238, 556, 620, 1537
Hpy188I	TCNGA	3 cut(s)	466, 829, 1431
Hpy188III	TCNNGA	9 cut(s)	290, 496, 773, 812, 911, 1070, 1436, 1800, 1861
Hpy8I	GTNNAC	4 cut(s)	238, 556, 620, 1537
Hpy99I	CGWCG	1 cut(s)	866
HpyAV	CCTTC	7 cut(s)	321, 1117, 1194, 1504, 1543, 1897, 2037
HpyCH4III	ACNGT	4 cut(s)	570, 1300, 1395, 1534
HpyCH4IV	ACGT	6 cut(s)	142, 580, 718, 1102, 1362, 1729
HpyF10VI	GCNNNNNNNGC	3 cut(s)	116, 1096, 2036
HpyF3I	CTNAG	2 cut(s)	315, 690
HpySE526I	ACGT	6 cut(s)	142, 580, 718, 1102, 1362, 1729
HspAI	GCGC	3 cut(s)	667, 1441, 1855
Kpn2I	TCCGGA	1 cut(s)	1799
Ksp22I	TGATCA	1 cut(s)	648
Kzo9I	GATC	7 cut(s)	648, 835, 1335, 1609, 1702, 1956, 1965
LmnI	GCTCC	3 cut(s)	107, 127, 895
Lsp1109I	GCAGC	6 cut(s)	293, 874, 1720, 2027, 2035, 2048
LweI	GCATC	6 cut(s)	703, 742, 1392, 1567, 1720, 1866
MaeI	CTAG	3 cut(s)	249, 1029, 1563
MaeII	ACGT	6 cut(s)	142, 580, 718, 1102, 1362, 1729
MaeIII	GTNAC	4 cut(s)	576, 685, 1169, 1690
MalI	GATC	7 cut(s)	650, 837, 1337, 1611, 1704, 1958, 1967
MbiI	CCGCTC	1 cut(s)	1378
MboI	GATC	7 cut(s)	648, 835, 1335, 1609, 1702, 1956, 1965
MflI	RGATCY	1 cut(s)	1956
MhlI	GDGCHC	1 cut(s)	630
MlsI	TGGCCA	1 cut(s)	1779
MluCI	AATT	8 cut(s)	678, 830, 1036, 1043, 1186, 1192, 1290, 1553
MluNI	TGGCCA	1 cut(s)	1779
MlyI	GAGTC	2 cut(s)	916, 1249
MmeI	TCCRAC	1 cut(s)	249
Mox20I	TGGCCA	1 cut(s)	1779
MroI	TCCGGA	1 cut(s)	1799
MroXI	GAANNNNTTC	1 cut(s)	1557
MscI	TGGCCA	1 cut(s)	1779
MseI	TTAA	8 cut(s)	375, 486, 840, 1008, 1017, 1151, 1191, 1770
MslI	CAYNNNNRTG	2 cut(s)	1233, 2031
Msp20I	TGGCCA	1 cut(s)	1779
MspA1I	CMGCKG	2 cut(s)	284, 1090
MspCI	CTTAAG	1 cut(s)	1007
MspI	CCGG	5 cut(s)	124, 278, 527, 1049, 1800
MspR9I	CCNGG	6 cut(s)	125, 278, 357, 726, 1049, 1050
Mva1269I	GAATGC	2 cut(s)	28, 589
MvaI	CCWGG	2 cut(s)	357, 726
MwoI	GCNNNNNNNGC	3 cut(s)	116, 1096, 2036
NciI	CCSGG	4 cut(s)	125, 278, 1049, 1050
NcoI	CCATGG	1 cut(s)	1158
NdeI	CATATG	2 cut(s)	1615, 1662
NdeII	GATC	7 cut(s)	648, 835, 1335, 1609, 1702, 1956, 1965
NlaIV	GGNNCC	5 cut(s)	321, 968, 1205, 1491, 1847
NmeAIII	GCCGAG	1 cut(s)	1273
NmuCI	GTSAC	2 cut(s)	576, 685
NsbI	TGCGCA	1 cut(s)	1856
NspI	RCATGY	4 cut(s)	188, 494, 1634, 2030
NspV	TTCGAA	1 cut(s)	593
PaeI	GCATGC	1 cut(s)	2030
PagI	TCATGA	2 cut(s)	772, 1435
PcsI	WCGNNNNNNNCGW	2 cut(s)	127, 867
PctI	GAATGC	2 cut(s)	28, 589
PdmI	GAANNNNTTC	1 cut(s)	1557
PfeI	GAWTC	9 cut(s)	64, 209, 332, 385, 518, 729, 1307, 1432, 1465
PflMI	CCANNNNNTGG	1 cut(s)	264
PfoI	TCCNGGA	1 cut(s)	724
PkrI	GCNGC	8 cut(s)	283, 889, 987, 1380, 1710, 2017, 2025, 2038
PleI	GAGTC	2 cut(s)	915, 1249
PpsI	GAGTC	2 cut(s)	915, 1249
PpuMI	RGGWCCY	2 cut(s)	881, 1846
Psp1406I	AACGTT	1 cut(s)	1102
Psp5II	RGGWCCY	2 cut(s)	881, 1846
Psp6I	CCWGG	2 cut(s)	355, 724
PspEI	GGTNACC	1 cut(s)	1690
PspFI	CCCAGC	1 cut(s)	2019
PspGI	CCWGG	2 cut(s)	355, 724
PspN4I	GGNNCC	5 cut(s)	321, 968, 1205, 1491, 1847
PspPI	GGNCC	5 cut(s)	881, 967, 1025, 1204, 1846
PspPPI	RGGWCCY	2 cut(s)	881, 1846
PstNI	CAGNNNCTG	2 cut(s)	38, 574
PsuI	RGATCY	1 cut(s)	1956
PvuII	CAGCTG	2 cut(s)	284, 1090
RsaI	GTAC	2 cut(s)	36, 619
RsaNI	GTAC	2 cut(s)	35, 618
RseI	CAYNNNNRTG	2 cut(s)	1233, 2031
SaqAI	TTAA	8 cut(s)	375, 486, 840, 1008, 1017, 1151, 1191, 1770
SatI	GCNGC	8 cut(s)	282, 888, 986, 1379, 1709, 2016, 2024, 2037
Sau3AI	GATC	7 cut(s)	648, 835, 1335, 1609, 1702, 1956, 1965
Sau96I	GGNCC	5 cut(s)	881, 967, 1025, 1204, 1846
SchI	GAGTC	2 cut(s)	916, 1249
ScrFI	CCNGG	6 cut(s)	125, 278, 357, 726, 1049, 1050
SduI	GDGCHC	1 cut(s)	630
SfaNI	GCATC	6 cut(s)	703, 742, 1392, 1567, 1720, 1866
SfcI	CTRYAG	2 cut(s)	1474, 1530
SfuI	TTCGAA	1 cut(s)	593
SinI	GGWCC	4 cut(s)	881, 967, 1204, 1846
SmaI	CCCGGG	1 cut(s)	1050
SmiMI	CAYNNNNRTG	2 cut(s)	1233, 2031
SmlI	CTYRAG	2 cut(s)	1007, 1406
SmoI	CTYRAG	2 cut(s)	1007, 1406
SphI	GCATGC	1 cut(s)	2030
Sse9I	AATT	8 cut(s)	678, 830, 1036, 1043, 1186, 1192, 1290, 1553
SsiI	CCGC	4 cut(s)	514, 985, 1378, 1624
SspI	AATATT	2 cut(s)	1450, 1655
SspMI	CTAG	3 cut(s)	249, 1029, 1563
StyD4I	CCNGG	6 cut(s)	123, 276, 355, 724, 1047, 1048
StyI	CCWWGG	3 cut(s)	962, 1158, 1562
TaaI	ACNGT	4 cut(s)	570, 1300, 1395, 1534
TaiI	ACGT	6 cut(s)	145, 583, 721, 1105, 1365, 1732
TaqI	TCGA	2 cut(s)	593, 1812
TasI	AATT	8 cut(s)	678, 830, 1036, 1043, 1186, 1192, 1290, 1553
TatI	WGTACW	1 cut(s)	617
TauI	GCSGC	2 cut(s)	988, 1381
TfiI	GAWTC	9 cut(s)	64, 209, 332, 385, 518, 729, 1307, 1432, 1465
Tru1I	TTAA	8 cut(s)	375, 486, 840, 1008, 1017, 1151, 1191, 1770
Tru9I	TTAA	8 cut(s)	375, 486, 840, 1008, 1017, 1151, 1191, 1770
TscAI	CASTG	1 cut(s)	186
TseFI	GTSAC	2 cut(s)	576, 685
TseI	GCWGC	6 cut(s)	281, 887, 1708, 2015, 2023, 2036
Tsp45I	GTSAC	2 cut(s)	576, 685
TspDTI	ATGAA	7 cut(s)	63, 186, 201, 377, 789, 1404, 1679
TspGWI	ACGGA	4 cut(s)	511, 673, 1767, 2014
TspMI	CCCGGG	1 cut(s)	1048
TspRI	CASTG	1 cut(s)	186
Van91I	CCANNNNNTGG	1 cut(s)	264
Vha464I	CTTAAG	1 cut(s)	1007
VpaK11BI	GGWCC	4 cut(s)	881, 967, 1204, 1846
XapI	RAATTY	4 cut(s)	830, 1036, 1186, 1553
XceI	RCATGY	4 cut(s)	188, 494, 1634, 2030
XcmI	CCANNNNNNNNNTGG	1 cut(s)	265
XmaI	CCCGGG	1 cut(s)	1048
XmaJI	CCTAGG	1 cut(s)	1562
XmiI	GTMKAC	1 cut(s)	555
XmnI	GAANNNNTTC	1 cut(s)	1557
XspI	CTAG	3 cut(s)	249, 1029, 1563

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.