Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0010990

Physical Location & Seq

Genomic Coordinates Forward (+)

23964861 .. 23976589

Gene Structure 11729 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1159800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2055 bp

ATGATTGCTGTCATGGACTTGAATGCCTCGCCAGTACCTGAAGAAGATGAGGATGCTTTTGAGAATCATATAGAATACGTTGCCCCAGAAGAACGTATAGAGAGTGGAGTTGATATAGCACGGCGGGAGCGTGAAGAAAGACGTAAGCGATTGAAACGAGAACGCCCAGATGAAAAACCAGTGCATGTTTCTCAACCCCCTGCATACGATTCATATCAAACAAAAAACCCCAAAGTTTACGACAAATCTAGGCTCCCCAATGGTTGGTTGGATTGTCCAGCAGCTGGTCAAGAAATATCTTTAATGATACCTTCTAAGGTGCCATTGGGTGAATCGTACAATGATTGTGTTCCTCCTGGTAAAAGATACTCATTTAAACAAGTGATTCATCAGCAAAGAGTTTTGGGAAGAAAACTTGGTTTGGTGATTGATTTGACAAATACTTCTCGCTATTATCTAACATCAGATTTGAAGAAAGAGGGTATTAAGCATGTCAAGATTGCGTGCAAGGGGCGGGATTCTGTGCCTGACAACCTCTCCGTAAATCAGTTTGTATACGAGGTTATACAGTTCCTGTCACGTCAAAAGCATTCGAAGAAATATATTCTTGTGCATTGTACACATGGGCACAATCGCACAGGGTATATGATCATCCACTACCTAATGCGCTCATTACCAATTTCAGTCACTCAGGCATTAAAAATTTTTGCGGATGCACGTCCTCCGGGAATCTACAAACCTGACTATGTTGATGCTTTATACTCATTCTATCATGAAAGAAAGCCTGATATGGTTGTCTGCCCCTCAACTCCTGAATGGAAGACTTCAGAGTTTGATCTTAATGGTGAAGCTGTTGCAGACGATGACGACGATGATGGGGGTCCTGCTGCTCCTGTAAATAATGAGAGTCTGGATACAAATGGATTGATGACAAATGATGATATCTTGGGCGATGAGATACCTTGGGACCAGCAGGAGGCAATGCGGCAGTTCTGCTATCAAACTCTTAAGCTGGTTAATGTTGGGCCTAGAGGAAACTCGCAATTCCCAGGGTCGCACCCCGTGTCTCTTAACAGGGAGAACTTACAGCTGTTAAGGCAACGTTATTATTATGCTACATGGAAGGCTGATGGAACACGATATATGATGTTAATAACGATGGATGGGTGTTACTTGGTTGATAGAAATTTTAATTTTAGAAGGGTCCAAATGAGGTTTCCCTGCAAACACACAAATGATGGTTTAGCCGAGAAGACTCACCACTACACATTACTTGATGGTGAGATGGTAATTGATACCGTGCCTGATTCACAGAAGCAGGAGAGAAGATACCTGATCTATGATATGATGGCAATCAACTACCTTTCTATCATAGAGCGGCCTTTTTATGAACGGTGGAAGATGCTTGAGAAAGAAGTCATTGAACCTCGGAATTATGAGCGCCACAATATATACCAAAGTAGGAATCCTTACTATAGATATGACCTGGAACCATTCAGGGTGAGGAGGAAGGACTTTTGGTTGCTCTCCACTGTAAACAAGCTTTTGAAGGAATTTATTCCAAGGCTTTCACATGATGCAGATGGTCTTATCTTTCAGGGTTGGGATGATCCTTATGTTCCCCGCACACATGAAGGACTTCTAAAGTGGAAATATTCTCATATGAACTCGGTTGATTTTCTCTTTGAGGTAACTGATGACGATTGGCGGCAGCATCTTTTTCTGTATGAACGTGGAAAGAAGAAACTAATGGACGGAAATAAAGTTGCGTTTAAAGATGGTGAAGACCCCACTTCTTATTCTGGAAAAATAATCGAGTGTTCTTGGAACGGTGAAGAACAAGAATGGGTCCTTATGCGCATCAGGACTGACAAATCAACTCCAAATGATATCAATACGTACAAGAAGGTCATGCGAAGCATAAAAGACAATATCACGCAGGACATCTTGTTGAATGAGATCTATGAGATCATTCGCCTGCCTATGTATGCTGATAGGATACGGAGTGATAGCAAAGCAGCCCACCAGCATGCAAATGCAGCACGAAGGAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004484 GO:0004651 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006370 GO:0006396 GO:0006397 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008192 GO:0009452 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010604 GO:0010628 GO:0016070 GO:0016071 GO:0016311 GO:0016462 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016791 GO:0016817 GO:0016818 GO:0019222 GO:0034641 GO:0036260 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048518 GO:0050355 GO:0050789 GO:0060255 GO:0065007 GO:0070568 GO:0071704 GO:0090304 GO:0098501 GO:0098507 GO:0098518 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

DSPc mRNA_cap_C mRNA_cap_enzyme

Protein Analysis

685

Amino Acids

80.36

Weight (kDa)

6.74

Isoelectric Point (pI)

47.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DSPc	PF00782	137 - 257	4.3e-15	Dual specificity phosphatase, catalytic domain
mRNA_cap_enzyme	PF01331	353 - 551	1.5e-75	mRNA capping enzyme, catalytic domain
mRNA_cap_C	PF03919	556 - 651	8.2e-20	mRNA capping enzyme, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010990)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09100 AT3G09100
fragaria_vesca	FvH4_6g12120 FvH4_6g12120 FvH4_6g12120
malus_domestica	MD04G1146600.v1.1 MD12G1159800.v1.1
prunus_persica	Prupe.6G270400_v2.0.a1
pyrus_communis	pycom04g13230 pycom12g15160
rosa_chinensis	RchiOBHm_Chr3g0463921
rosa_laevigata	RLG00000024736
rosa_multiflora	Rmu_sc0000517.1_g000001
rosa_roxburghii	Rroxscaffold_6G00416140
rosa_rugosa	Rorug03G0069200
rosa_samantha	Rh3AG126200 Rh3BG130200 Rh3CG132700 Rh3DG131400
rosa_wichuraiana	Rw3G010650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1859
AccB1I	GGYRCC	1 cut(s)	319
AccB7I	CCANNNNNTGG	2 cut(s)	264, 284
AccBSI	CCGCTC	1 cut(s)	1378
AccI	GTMKAC	1 cut(s)	555
AciI	CCGC	7 cut(s)	124, 514, 710, 985, 1378, 1624, 1708
AclI	AACGTT	1 cut(s)	1102
AclWI	GGATC	1 cut(s)	1604
AcsI	RAATTY	3 cut(s)	702, 1186, 1553
AcuI	CTGAAG	2 cut(s)	60, 810
AdeI	CACNNNGTG	1 cut(s)	1063
AfaI	GTAC	4 cut(s)	36, 338, 619, 1901
AfiI	CCNNNNNNNGG	3 cut(s)	264, 284, 976
AflII	CTTAAG	1 cut(s)	1007
AgsI	TTSAA	6 cut(s)	22, 154, 472, 1424, 1549, 1954
AjiI	CACGTC	2 cut(s)	581, 719
AjnI	CCWGG	3 cut(s)	355, 1048, 1485
AjuI	GAANNNNNNNTTGG	2 cut(s)	1200, 1232
AluBI	AGCT	5 cut(s)	284, 851, 1012, 1090, 1543
AluI	AGCT	5 cut(s)	284, 851, 1012, 1090, 1543
Alw26I	GTCTC	1 cut(s)	1071
AlwI	GGATC	1 cut(s)	1604
AlwNI	CAGNNNCTG	3 cut(s)	38, 284, 574
AoxI	GGCC	2 cut(s)	1025, 1379
ApeKI	GCWGC	5 cut(s)	281, 887, 1711, 2018, 2039
ApoI	RAATTY	3 cut(s)	702, 1186, 1553
Asp700I	GAANNNNTTC	2 cut(s)	1557, 1638
AspLEI	GCGC	3 cut(s)	669, 1443, 1860
AspS9I	GGNCC	5 cut(s)	881, 967, 1025, 1204, 1849
AsuC2I	CCSGG	1 cut(s)	726
AsuHPI	GGTGA	8 cut(s)	341, 436, 857, 1250, 1292, 1513, 1793, 1844
AsuII	TTCGAA	1 cut(s)	593
AvaII	GGWCC	4 cut(s)	881, 967, 1204, 1849
BaeGI	GKGCMC	1 cut(s)	630
BanI	GGYRCC	1 cut(s)	319
BbsI	GAAGAC	3 cut(s)	827, 1259, 1791
BbvI	GCAGC	5 cut(s)	293, 874, 1723, 2030, 2051
BceAI	ACGGC	1 cut(s)	137
BciT130I	CCWGG	3 cut(s)	357, 1050, 1487
BciVI	GTATCC	2 cut(s)	907, 1992
BclI	TGATCA	1 cut(s)	648
BcnI	CCSGG	1 cut(s)	726
BcoDI	GTCTC	1 cut(s)	1071
BfaI	CTAG	2 cut(s)	249, 1029
BfmI	CTRYAG	1 cut(s)	1474
BfoI	RGCGCY	1 cut(s)	1444
BfrI	CTTAAG	1 cut(s)	1007
BfuI	GTATCC	2 cut(s)	907, 1992
BglII	AGATCT	1 cut(s)	1959
BisI	GCNGC	8 cut(s)	282, 888, 986, 1379, 1709, 1712, 2019, 2040
BlsI	GCNGC	8 cut(s)	283, 889, 987, 1380, 1710, 1713, 2020, 2041
Bme1390I	CCNGG	4 cut(s)	357, 726, 1050, 1487
Bme18I	GGWCC	4 cut(s)	881, 967, 1204, 1849
BmgBI	CACGTC	2 cut(s)	581, 719
BmgT120I	GGNCC	5 cut(s)	881, 967, 1025, 1204, 1849
BmiI	GGNNCC	7 cut(s)	254, 321, 882, 968, 1205, 1491, 1850
BmrFI	CCNGG	4 cut(s)	357, 726, 1050, 1487
BmsI	GCATC	7 cut(s)	43, 703, 742, 1392, 1567, 1723, 1869
BpiI	GAAGAC	3 cut(s)	827, 1259, 1791
Bpu14I	TTCGAA	1 cut(s)	593
BpuEI	CTTGAG	1 cut(s)	1427
BpuMI	CCSGG	1 cut(s)	726
BsaAI	YACGTR	1 cut(s)	1899
BsaBI	GATNNNNATC	2 cut(s)	213, 1352
BsaJI	CCNNGG	5 cut(s)	962, 1048, 1049, 1427, 1562
BsaXI	ACNNNNNCTCC	2 cut(s)	521, 551
Bsc4I	CCNNNNNNNGG	3 cut(s)	264, 284, 976
Bse1I	ACTGG	2 cut(s)	32, 179
Bse3DI	GCAATG	1 cut(s)	987
Bse8I	GATNNNNATC	2 cut(s)	213, 1352
BseBI	CCWGG	3 cut(s)	357, 1050, 1487
BseDI	CCNNGG	5 cut(s)	962, 1048, 1049, 1427, 1562
BseGI	GGATG	5 cut(s)	58, 651, 718, 1168, 1612
BseJI	GATNNNNATC	2 cut(s)	213, 1352
BseLI	CCNNNNNNNGG	3 cut(s)	264, 284, 976
BseMI	GCAATG	1 cut(s)	987
BseMII	CTCAG	1 cut(s)	704
BseNI	ACTGG	2 cut(s)	32, 179
BseRI	GAGGAG	1 cut(s)	1519
BseSI	GKGCMC	1 cut(s)	630
BseXI	GCAGC	5 cut(s)	293, 874, 1723, 2030, 2051
BshFI	GGCC	2 cut(s)	1027, 1381
BshNI	GGYRCC	1 cut(s)	319
BsiSI	CCGG	1 cut(s)	725
BslFI	GGGAC	1 cut(s)	980
BslI	CCNNNNNNNGG	3 cut(s)	264, 284, 976
BsmAI	GTCTC	1 cut(s)	1071
BsmFI	GGGAC	1 cut(s)	980
BsmI	GAATGC	2 cut(s)	28, 589
BsnI	GGCC	2 cut(s)	1027, 1381
Bsp119I	TTCGAA	1 cut(s)	593
Bsp1286I	GDGCHC	1 cut(s)	630
Bsp1407I	TGTACA	1 cut(s)	617
Bsp143I	GATC	6 cut(s)	648, 835, 1335, 1609, 1959, 1968
BspACI	CCGC	7 cut(s)	124, 514, 710, 985, 1378, 1624, 1708
BspANI	GGCC	2 cut(s)	1027, 1381
BspCNI	CTCAG	1 cut(s)	703
BspHI	TCATGA	1 cut(s)	772
BspLI	GGNNCC	7 cut(s)	254, 321, 882, 968, 1205, 1491, 1850
BspPI	GGATC	1 cut(s)	1604
BspT104I	TTCGAA	1 cut(s)	593
BspT107I	GGYRCC	1 cut(s)	319
BspTI	CTTAAG	1 cut(s)	1007
BsrBI	CCGCTC	1 cut(s)	1378
BsrDI	GCAATG	1 cut(s)	987
BsrGI	TGTACA	1 cut(s)	617
BsrI	ACTGG	2 cut(s)	32, 179
BssECI	CCNNGG	5 cut(s)	962, 1048, 1049, 1427, 1562
BssMI	GATC	6 cut(s)	648, 835, 1335, 1609, 1959, 1968
BssNAI	GTATAC	1 cut(s)	556
BssT1I	CCWWGG	2 cut(s)	962, 1562
Bst1107I	GTATAC	1 cut(s)	556
Bst2UI	CCWGG	3 cut(s)	357, 1050, 1487
Bst4CI	ACNGT	5 cut(s)	570, 1300, 1395, 1534, 1832
BstAFI	CTTAAG	1 cut(s)	1007
BstAUI	TGTACA	1 cut(s)	617
BstBAI	YACGTR	1 cut(s)	1899
BstBI	TTCGAA	1 cut(s)	593
BstC8I	GCNNGC	3 cut(s)	505, 1979, 2031
BstDEI	CTNAG	2 cut(s)	315, 690
BstF5I	GGATG	5 cut(s)	58, 651, 718, 1168, 1612
BstH2I	RGCGCY	1 cut(s)	1444
BstHHI	GCGC	3 cut(s)	669, 1443, 1860
BstKTI	GATC	6 cut(s)	651, 838, 1338, 1612, 1962, 1971
BstMAI	GTCTC	1 cut(s)	1071
BstMBI	GATC	6 cut(s)	648, 835, 1335, 1609, 1959, 1968
BstMWI	GCNNNNNNNGC	2 cut(s)	1096, 2039
BstNI	CCWGG	3 cut(s)	357, 1050, 1487
BstNSI	RCATGY	3 cut(s)	188, 494, 2033
BstSCI	CCNGG	4 cut(s)	355, 724, 1048, 1485
BstSFI	CTRYAG	1 cut(s)	1474
BstSLI	GKGCMC	1 cut(s)	630
BstSNI	TACGTA	1 cut(s)	1899
BstV1I	GCAGC	5 cut(s)	293, 874, 1723, 2030, 2051
BstV2I	GAAGAC	3 cut(s)	827, 1259, 1791
BstX2I	RGATCY	1 cut(s)	1959
BstYI	RGATCY	1 cut(s)	1959
BstZ17I	GTATAC	1 cut(s)	556
BsuI	GTATCC	2 cut(s)	907, 1992
BsuRI	GGCC	2 cut(s)	1027, 1381
BtgZI	GCGATG	1 cut(s)	966
BtrI	CACGTC	2 cut(s)	581, 719
BtsCI	GGATG	5 cut(s)	58, 651, 718, 1168, 1612
BtsIMutI	CAGTG	2 cut(s)	186, 1530
Cac8I	GCNNGC	3 cut(s)	505, 1979, 2031
CaiI	CAGNNNCTG	3 cut(s)	38, 284, 574
CciI	TCATGA	1 cut(s)	772
CfoI	GCGC	3 cut(s)	669, 1443, 1860
Cfr13I	GGNCC	5 cut(s)	881, 967, 1025, 1204, 1849
Csp6I	GTAC	4 cut(s)	35, 337, 618, 1900
CviQI	GTAC	4 cut(s)	35, 337, 618, 1900
DdeI	CTNAG	2 cut(s)	315, 690
DpnI	GATC	6 cut(s)	650, 837, 1337, 1611, 1961, 1970
DpnII	GATC	6 cut(s)	648, 835, 1335, 1609, 1959, 1968
DraI	TTTAAA	2 cut(s)	376, 1774
DraIII	CACNNNGTG	1 cut(s)	1063
Eco105I	TACGTA	1 cut(s)	1899
Eco130I	CCWWGG	2 cut(s)	962, 1562
Eco32I	GATATC	2 cut(s)	943, 1891
Eco47I	GGWCC	4 cut(s)	881, 967, 1204, 1849
Eco57I	CTGAAG	2 cut(s)	60, 810
EcoO109I	RGGNCCY	2 cut(s)	881, 1849
EcoRII	CCWGG	3 cut(s)	355, 1048, 1485
EcoRV	GATATC	2 cut(s)	943, 1891
EcoT14I	CCWWGG	2 cut(s)	962, 1562
ErhI	CCWWGG	2 cut(s)	962, 1562
FaqI	GGGAC	1 cut(s)	980
FauI	CCCGC	3 cut(s)	117, 507, 1631
FauNDI	CATATG	1 cut(s)	1662
FbaI	TGATCA	1 cut(s)	648
FblI	GTMKAC	1 cut(s)	555
Fnu4HI	GCNGC	8 cut(s)	282, 888, 986, 1379, 1709, 1712, 2019, 2040
FokI	GGATG	5 cut(s)	65, 638, 725, 1175, 1619
Fsp4HI	GCNGC	8 cut(s)	282, 888, 986, 1379, 1709, 1712, 2019, 2040
FspAI	RTGCGCAY	1 cut(s)	1859
FspBI	CTAG	2 cut(s)	249, 1029
FspI	TGCGCA	1 cut(s)	1859
GlaI	GCGC	3 cut(s)	668, 1442, 1859
GluI	GCNGC	8 cut(s)	282, 888, 986, 1379, 1709, 1712, 2019, 2040
HaeII	RGCGCY	1 cut(s)	1444
HaeIII	GGCC	2 cut(s)	1027, 1381
HapII	CCGG	1 cut(s)	725
HhaI	GCGC	3 cut(s)	669, 1443, 1860
Hin6I	GCGC	3 cut(s)	667, 1441, 1858
HinP1I	GCGC	3 cut(s)	667, 1441, 1858
HindIII	AAGCTT	1 cut(s)	1541
HpaII	CCGG	1 cut(s)	725
HphI	GGTGA	8 cut(s)	341, 436, 857, 1250, 1292, 1513, 1793, 1844
Hpy166II	GTNNAC	4 cut(s)	238, 556, 620, 1537
Hpy188I	TCNGA	3 cut(s)	466, 829, 1431
Hpy188III	TCNNGA	7 cut(s)	290, 496, 773, 812, 911, 1803, 1864
Hpy8I	GTNNAC	4 cut(s)	238, 556, 620, 1537
Hpy99I	CGWCG	1 cut(s)	872
HpyAV	CCTTC	8 cut(s)	321, 1117, 1194, 1504, 1543, 1628, 1900, 2040
HpyCH4III	ACNGT	5 cut(s)	570, 1300, 1395, 1534, 1832
HpyCH4IV	ACGT	8 cut(s)	78, 94, 142, 580, 718, 1102, 1732, 1898
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1096, 2039
HpyF3I	CTNAG	2 cut(s)	315, 690
HpySE526I	ACGT	8 cut(s)	78, 94, 142, 580, 718, 1102, 1732, 1898
HspAI	GCGC	3 cut(s)	667, 1441, 1858
Ksp22I	TGATCA	1 cut(s)	648
Kzo9I	GATC	6 cut(s)	648, 835, 1335, 1609, 1959, 1968
LmnI	GCTCC	3 cut(s)	127, 258, 895
Lsp1109I	GCAGC	5 cut(s)	293, 874, 1723, 2030, 2051
LweI	GCATC	7 cut(s)	43, 703, 742, 1392, 1567, 1723, 1869
MaeI	CTAG	2 cut(s)	249, 1029
MaeII	ACGT	8 cut(s)	78, 94, 142, 580, 718, 1102, 1732, 1898
MaeIII	GTNAC	4 cut(s)	576, 685, 1169, 1690
MalI	GATC	6 cut(s)	650, 837, 1337, 1611, 1961, 1970
MbiI	CCGCTC	1 cut(s)	1378
MboI	GATC	6 cut(s)	648, 835, 1335, 1609, 1959, 1968
MflI	RGATCY	1 cut(s)	1959
MhlI	GDGCHC	1 cut(s)	630
MluCI	AATT	8 cut(s)	678, 702, 1043, 1186, 1192, 1290, 1432, 1553
MlyI	GAGTC	2 cut(s)	916, 1249
MmeI	TCCRAC	1 cut(s)	249
MroXI	GAANNNNTTC	2 cut(s)	1557, 1638
MslI	CAYNNNNRTG	3 cut(s)	1233, 2028, 2034
MspA1I	CMGCKG	2 cut(s)	284, 1090
MspCI	CTTAAG	1 cut(s)	1007
MspI	CCGG	1 cut(s)	725
MspR9I	CCNGG	4 cut(s)	357, 726, 1050, 1487
Mva1269I	GAATGC	2 cut(s)	28, 589
MvaI	CCWGG	3 cut(s)	357, 1050, 1487
MwoI	GCNNNNNNNGC	2 cut(s)	1096, 2039
NciI	CCSGG	1 cut(s)	726
NdeI	CATATG	1 cut(s)	1662
NdeII	GATC	6 cut(s)	648, 835, 1335, 1609, 1959, 1968
NlaIV	GGNNCC	7 cut(s)	254, 321, 882, 968, 1205, 1491, 1850
NmeAIII	GCCGAG	1 cut(s)	1273
NmuCI	GTSAC	2 cut(s)	576, 685
NsbI	TGCGCA	1 cut(s)	1859
NspI	RCATGY	3 cut(s)	188, 494, 2033
NspV	TTCGAA	1 cut(s)	593
PaeI	GCATGC	1 cut(s)	2033
PagI	TCATGA	1 cut(s)	772
PasI	CCCWGGG	1 cut(s)	1049
PcsI	WCGNNNNNNNCGW	2 cut(s)	127, 867
PctI	GAATGC	2 cut(s)	28, 589
PdmI	GAANNNNTTC	2 cut(s)	1557, 1638
PfeI	GAWTC	8 cut(s)	64, 209, 332, 385, 518, 729, 1307, 1465
PflMI	CCANNNNNTGG	2 cut(s)	264, 284
PfoI	TCCNGGA	1 cut(s)	724
PkrI	GCNGC	8 cut(s)	283, 889, 987, 1380, 1710, 1713, 2020, 2041
PleI	GAGTC	2 cut(s)	915, 1249
PpsI	GAGTC	2 cut(s)	915, 1249
Ppu21I	YACGTR	1 cut(s)	1899
PpuMI	RGGWCCY	2 cut(s)	881, 1849
Psp1406I	AACGTT	1 cut(s)	1102
Psp5II	RGGWCCY	2 cut(s)	881, 1849
Psp6I	CCWGG	3 cut(s)	355, 1048, 1485
PspGI	CCWGG	3 cut(s)	355, 1048, 1485
PspN4I	GGNNCC	7 cut(s)	254, 321, 882, 968, 1205, 1491, 1850
PspPI	GGNCC	5 cut(s)	881, 967, 1025, 1204, 1849
PspPPI	RGGWCCY	2 cut(s)	881, 1849
PstNI	CAGNNNCTG	3 cut(s)	38, 284, 574
PsuI	RGATCY	1 cut(s)	1959
PvuII	CAGCTG	2 cut(s)	284, 1090
RsaI	GTAC	4 cut(s)	36, 338, 619, 1901
RsaNI	GTAC	4 cut(s)	35, 337, 618, 1900
RseI	CAYNNNNRTG	3 cut(s)	1233, 2028, 2034
SatI	GCNGC	8 cut(s)	282, 888, 986, 1379, 1709, 1712, 2019, 2040
Sau3AI	GATC	6 cut(s)	648, 835, 1335, 1609, 1959, 1968
Sau96I	GGNCC	5 cut(s)	881, 967, 1025, 1204, 1849
SchI	GAGTC	2 cut(s)	916, 1249
ScrFI	CCNGG	4 cut(s)	357, 726, 1050, 1487
SduI	GDGCHC	1 cut(s)	630
SfaNI	GCATC	7 cut(s)	43, 703, 742, 1392, 1567, 1723, 1869
SfcI	CTRYAG	1 cut(s)	1474
SfuI	TTCGAA	1 cut(s)	593
SinI	GGWCC	4 cut(s)	881, 967, 1204, 1849
SmiMI	CAYNNNNRTG	3 cut(s)	1233, 2028, 2034
SmlI	CTYRAG	2 cut(s)	1007, 1406
SmoI	CTYRAG	2 cut(s)	1007, 1406
SnaBI	TACGTA	1 cut(s)	1899
SphI	GCATGC	1 cut(s)	2033
Sse9I	AATT	8 cut(s)	678, 702, 1043, 1186, 1192, 1290, 1432, 1553
SsiI	CCGC	7 cut(s)	124, 514, 710, 985, 1378, 1624, 1708
SspI	AATATT	1 cut(s)	1655
SspMI	CTAG	2 cut(s)	249, 1029
StyD4I	CCNGG	4 cut(s)	355, 724, 1048, 1485
StyI	CCWWGG	2 cut(s)	962, 1562
TaaI	ACNGT	5 cut(s)	570, 1300, 1395, 1534, 1832
TaiI	ACGT	8 cut(s)	81, 97, 145, 583, 721, 1105, 1735, 1901
TaqI	TCGA	2 cut(s)	593, 1815
TasI	AATT	8 cut(s)	678, 702, 1043, 1186, 1192, 1290, 1432, 1553
TatI	WGTACW	1 cut(s)	617
TauI	GCSGC	3 cut(s)	988, 1381, 1711
TfiI	GAWTC	8 cut(s)	64, 209, 332, 385, 518, 729, 1307, 1465
TscAI	CASTG	2 cut(s)	186, 1537
TseFI	GTSAC	2 cut(s)	576, 685
TseI	GCWGC	5 cut(s)	281, 887, 1711, 2018, 2039
Tsp45I	GTSAC	2 cut(s)	576, 685
TspDTI	ATGAA	8 cut(s)	186, 201, 377, 789, 1404, 1647, 1679, 1743
TspGWI	ACGGA	3 cut(s)	529, 1770, 2017
TspRI	CASTG	2 cut(s)	186, 1537
Van91I	CCANNNNNTGG	2 cut(s)	264, 284
Vha464I	CTTAAG	1 cut(s)	1007
VpaK11BI	GGWCC	4 cut(s)	881, 967, 1204, 1849
XapI	RAATTY	3 cut(s)	702, 1186, 1553
XceI	RCATGY	3 cut(s)	188, 494, 2033
XcmI	CCANNNNNNNNNTGG	1 cut(s)	265
XmiI	GTMKAC	1 cut(s)	555
XmnI	GAANNNNTTC	2 cut(s)	1557, 1638
XspI	CTAG	2 cut(s)	249, 1029

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.