Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0013246

Physical Location & Seq

Genomic Coordinates Forward (+)

26706169 .. 26708646

Gene Structure 2478 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1176200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1506 bp

ATGGCCTATCTGCACTCTCCAAGTCTATCCACACGTAAACACAACCCTAAGCCTGCATGCTTCCCTCCTTCAAAACCCTCTTCACTTCACAATCTCTGCTGCTTCACAACCCAACTCAGAATCTCCCCTCACAGATGCAATGCATTCTTCGGCGAAATCCCTGAAAATCTTATGGAAACTACCCTCCATTTGGACCAATTTCCTCCTCTGCAATATGGTTTCATGCAGTTTGAGAGAATTACAGAAGAGCTGTCAGAAACACAGAAATGGGGTTTCCTTGTTTTTGCTGGGATTACTTGGATATACTTGACCGCAAGGCCGGGGATTCTCATTGGCGCCATTGATGCATACCTTCTTGCTCCTCTGCAGTTGGGGTTGGATAGTTTGACTGGGAGGAGGAGGTTGAAAAGGACGGGTTTTGTGATCGGGGAAAAATTGGGAGAAGGGTCTTTTGGTGTGGTTTATTCCGGGGCAGTTGTTCCGAAGAATGTGAATGTCGAGGAGACTGTGCAGAGGAGAGGAATGGGGAGGGCTCCACAGTTGGATGAGAGGTTTAAGGAGAAGGTCATCCTGAAGAAGGTGAAGATTGGTGTTCAAGGGGCTGAAGATTTTGGCGAATTCGAGGAGTGGTTCAATTACAGGTTGTCTAGAGCAGCACCTGAAACATGTGCTGCGTTCTTGGGAAGTTTCGTTGCTGATAAAACAAAGTCACAGTTTACAAAGGGTGGAAAGTGGCTCGTTTGGAAATTCGAGGGAAACCAAAACCTTGATGATTACATGAAAGATAGGAAATTCCCTTTTAACTTAGAGTCTGTCATGTTTGGACGTGTCCTGCAAGATGTAGACTCCGTCAGACGCAGTGCATTGATCATCAAGCAAATAATGCGTCAAATTATTACTTCGCTTAAGAAAATCCATGACACGGGCATTGTTCATCGGGATGTAAAACCAGCAAACTTAGTAGTGACGAAGAAGGGACAGATCAAGCTCATAGATTTCGGGGCAGCCACTGACCTCCGGATTGGAAAGAATTATATACCAGACTGCACCCTGCTAGACCCTGACTTTTGTCCTCCTGAACTATATGTGCTCCCAGAGGACACACCAAATCTTCCTCCCGAGCCTATTGCTGCATTACTTTCTCCGGTTCTTTGGCAGCTCAACAGTCCTGATCTGTTTGATATGTATTCTGCTGGAATTATACTATTGCAATTGGCAATACCATCCTTAAGGTCTTCAGCAGGCTTAAAGAATTTCAATTCAGAGATAAAAATAGTTAAATATGACTTGAATAAATGGAAGGACTGCACTCGGTTGAGACCTGACTTGTCACTTCTTGATCTCGATTCGGGTAGAGGCTGGGATCTGGCCACAAAGCTTGTTTCCGAGAGAGGTTCTCTTAGAAGGGGGCGTTTATCAGCGGCCGCTGCTCTGAGGCATCCTTATTTCTTGTTAGCTGGTGACCAGGCAGCTGCAGTTCTTTCAAAACTAAGTTTTACCAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009579 GO:0009987 GO:0010109 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0031323 GO:0036211 GO:0042548 GO:0042549 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043467 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

502

Amino Acids

56.31

Weight (kDa)

9.24

Isoelectric Point (pI)

42.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	142 - 360	3.9e-10	Protein tyrosine and serine/threonine kinase
ABC1	PF03109	275 - 339	8.5e-08	ABC1 atypical kinase-like domain
Pkinase	PF00069	286 - 483	3.7e-19	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013246)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01920 AT5G01920
fragaria_vesca	FvH4_6g09630
malus_domestica	MD04G1176200.v1.1 MD12G1190500.v1.1
prunus_persica	Prupe.6G295600_v2.0.a1
pyrus_communis	pycom04g15540
rosa_chinensis	RchiOBHm_Chr3g0460271
rosa_laevigata	RLG00000025029
rosa_multiflora	Rmu_co8344035.1_g000001 Rmu_sc0003763.1_g000001 Rmu_sc0035433.1_g000001
rosa_rugosa	Rorug03G0041200
rosa_samantha	Rh3AG099100 Rh3BG103000 Rh3CG104600 Rh3DG104000
rosa_wichuraiana	Rw3G008400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	335
AccI	GTMKAC	1 cut(s)	843
AccIII	TCCGGA	1 cut(s)	1017
AciI	CCGC	3 cut(s)	312, 1421, 1425
AclWI	GGATC	1 cut(s)	1371
AcoI	YGGCCR	2 cut(s)	1368, 1422
AcsI	RAATTY	4 cut(s)	617, 746, 791, 1252
AcuI	CTGAAG	3 cut(s)	593, 624, 1221
AcyI	GRCGYC	1 cut(s)	336
AfiI	CCNNNNNNNGG	3 cut(s)	190, 577, 922
AflII	CTTAAG	2 cut(s)	905, 1228
AflIII	ACRYGT	3 cut(s)	32, 665, 826
AgsI	TTSAA	7 cut(s)	72, 406, 596, 634, 1258, 1291, 1485
AjiI	CACGTC	1 cut(s)	827
AjnI	CCWGG	1 cut(s)	1464
AjuI	GAANNNNNNNTTGG	2 cut(s)	435, 467
AluBI	AGCT	6 cut(s)	250, 988, 1159, 1378, 1457, 1472
AluI	AGCT	6 cut(s)	250, 988, 1159, 1378, 1457, 1472
Alw21I	GWGCWC	1 cut(s)	1092
Alw26I	GTCTC	2 cut(s)	497, 1312
AlwI	GGATC	1 cut(s)	1371
AlwNI	CAGNNNCTG	2 cut(s)	659, 1010
Ama87I	CYCGRG	1 cut(s)	1118
Aor13HI	TCCGGA	1 cut(s)	1017
AoxI	GGCC	4 cut(s)	3, 317, 1368, 1422
ApeKI	GCWGC	9 cut(s)	99, 653, 671, 1004, 1130, 1156, 1427, 1469, 1472
ApoI	RAATTY	4 cut(s)	617, 746, 791, 1252
ArsI	GACNNNNNNTTYG	2 cut(s)	871, 903
AspLEI	GCGC	1 cut(s)	338
AspS9I	GGNCC	1 cut(s)	193
AsuC2I	CCSGG	2 cut(s)	321, 469
AsuHPI	GGTGA	2 cut(s)	592, 1472
AvaI	CYCGRG	1 cut(s)	1118
AvaII	GGWCC	1 cut(s)	193
BalI	TGGCCA	1 cut(s)	1370
BanI	GGYRCC	1 cut(s)	335
BanII	GRGCYC	1 cut(s)	535
BbsI	GAAGAC	1 cut(s)	1227
Bbv12I	GWGCWC	1 cut(s)	1092
BbvI	GCAGC	9 cut(s)	86, 658, 665, 1016, 1117, 1168, 1414, 1459, 1481
BccI	CCATC	1 cut(s)	1231
BcgI	CGANNNNNNTGC	2 cut(s)	1109, 1143
BciT130I	CCWGG	1 cut(s)	1466
BclI	TGATCA	1 cut(s)	867
BcnI	CCSGG	2 cut(s)	321, 469
BcoDI	GTCTC	2 cut(s)	497, 1312
BfaI	CTAG	2 cut(s)	648, 1055
BfmI	CTRYAG	2 cut(s)	365, 1473
BfoI	RGCGCY	1 cut(s)	339
BfrI	CTTAAG	2 cut(s)	905, 1228
Bme1390I	CCNGG	3 cut(s)	321, 469, 1466
Bme18I	GGWCC	1 cut(s)	193
BmeT110I	CYCGRG	1 cut(s)	1118
BmgBI	CACGTC	1 cut(s)	827
BmgT120I	GGNCC	1 cut(s)	193
BmiI	GGNNCC	2 cut(s)	337, 534
BmrFI	CCNGG	3 cut(s)	321, 469, 1466
BmrI	ACTGGG	1 cut(s)	399
BmsI	GCATC	3 cut(s)	125, 334, 1447
BmuI	ACTGGG	1 cut(s)	399
BoxI	GACNNNNGTC	1 cut(s)	1068
BpiI	GAAGAC	1 cut(s)	1227
BplI	GAGNNNNNCTC	2 cut(s)	1381, 1413
Bpu10I	CCTNAGC	1 cut(s)	48
BpuMI	CCSGG	2 cut(s)	321, 469
BsaAI	YACGTR	1 cut(s)	35
BsaHI	GRCGYC	1 cut(s)	336
BsaI	GGTCTC	1 cut(s)	1312
BsaJI	CCNNGG	2 cut(s)	320, 468
BsaWI	WCCGGW	2 cut(s)	1017, 1144
Bsc4I	CCNNNNNNNGG	3 cut(s)	190, 577, 922
Bse1I	ACTGG	1 cut(s)	394
Bse3DI	GCAATG	1 cut(s)	145
BseAI	TCCGGA	1 cut(s)	1017
BseBI	CCWGG	1 cut(s)	1466
BseDI	CCNNGG	2 cut(s)	320, 468
BseGI	GGATG	5 cut(s)	550, 567, 946, 1223, 1438
BseLI	CCNNNNNNNGG	3 cut(s)	190, 577, 922
BseMI	GCAATG	1 cut(s)	145
BseMII	CTCAG	2 cut(s)	130, 1424
BseNI	ACTGG	1 cut(s)	394
BseRI	GAGGAG	7 cut(s)	195, 351, 409, 412, 515, 529, 638
BseX3I	CGGCCG	1 cut(s)	1422
BseXI	GCAGC	9 cut(s)	86, 658, 665, 1016, 1117, 1168, 1414, 1459, 1481
BseYI	CCCAGC	2 cut(s)	287, 1359
BsgI	GTGCAG	3 cut(s)	530, 1030, 1291
Bsh1285I	CGRYCG	1 cut(s)	1425
BshFI	GGCC	4 cut(s)	5, 319, 1370, 1424
BshNI	GGYRCC	1 cut(s)	335
BsiEI	CGRYCG	1 cut(s)	1425
BsiHKAI	GWGCWC	1 cut(s)	1092
BsiHKCI	CYCGRG	1 cut(s)	1118
BsiSI	CCGG	4 cut(s)	320, 468, 1018, 1145
BslFI	GGGAC	1 cut(s)	990
BslI	CCNNNNNNNGG	3 cut(s)	190, 577, 922
BsmAI	GTCTC	2 cut(s)	497, 1312
BsmFI	GGGAC	1 cut(s)	990
BsmI	GAATGC	1 cut(s)	143
BsnI	GGCC	4 cut(s)	5, 319, 1370, 1424
Bso31I	GGTCTC	1 cut(s)	1312
BsoBI	CYCGRG	1 cut(s)	1118
Bsp1286I	GDGCHC	2 cut(s)	535, 1092
Bsp13I	TCCGGA	1 cut(s)	1017
Bsp143I	GATC	6 cut(s)	423, 867, 981, 1171, 1339, 1363
BspACI	CCGC	3 cut(s)	312, 1421, 1425
BspANI	GGCC	4 cut(s)	5, 319, 1370, 1424
BspCNI	CTCAG	2 cut(s)	129, 1425
BspEI	TCCGGA	1 cut(s)	1017
BspLI	GGNNCC	2 cut(s)	337, 534
BspMAI	CTGCAG	2 cut(s)	369, 1477
BspPI	GGATC	1 cut(s)	1371
BspQI	GCTCTTC	1 cut(s)	240
BspT107I	GGYRCC	1 cut(s)	335
BspTI	CTTAAG	2 cut(s)	905, 1228
BspTNI	GGTCTC	1 cut(s)	1312
BsrDI	GCAATG	1 cut(s)	145
BsrI	ACTGG	1 cut(s)	394
BssECI	CCNNGG	2 cut(s)	320, 468
BssMI	GATC	6 cut(s)	423, 867, 981, 1171, 1339, 1363
BssNI	GRCGYC	1 cut(s)	336
Bst2UI	CCWGG	1 cut(s)	1466
Bst4CI	ACNGT	4 cut(s)	508, 540, 714, 1166
Bst6I	CTCTTC	2 cut(s)	85, 240
BstACI	GRCGYC	1 cut(s)	336
BstAFI	CTTAAG	2 cut(s)	905, 1228
BstAPI	GCANNNNNTGC	1 cut(s)	883
BstBAI	YACGTR	1 cut(s)	35
BstC8I	GCNNGC	3 cut(s)	54, 58, 1243
BstDEI	CTNAG	7 cut(s)	48, 116, 805, 958, 1400, 1433, 1490
BstEII	GGTNACC	1 cut(s)	1460
BstENI	CCTNNNNNAGG	1 cut(s)	575
BstF5I	GGATG	5 cut(s)	550, 567, 946, 1223, 1438
BstH2I	RGCGCY	1 cut(s)	339
BstHHI	GCGC	1 cut(s)	338
BstKTI	GATC	6 cut(s)	426, 870, 984, 1174, 1342, 1366
BstMAI	GTCTC	2 cut(s)	497, 1312
BstMBI	GATC	6 cut(s)	423, 867, 981, 1171, 1339, 1363
BstMCI	CGRYCG	1 cut(s)	1425
BstMWI	GCNNNNNNNGC	3 cut(s)	344, 883, 1427
BstNI	CCWGG	1 cut(s)	1466
BstNSI	RCATGY	2 cut(s)	60, 669
BstPAI	GACNNNNGTC	1 cut(s)	1068
BstPI	GGTNACC	1 cut(s)	1460
BstSCI	CCNGG	3 cut(s)	319, 467, 1464
BstSFI	CTRYAG	2 cut(s)	365, 1473
BstV1I	GCAGC	9 cut(s)	86, 658, 665, 1016, 1117, 1168, 1414, 1459, 1481
BstV2I	GAAGAC	1 cut(s)	1227
BstX2I	RGATCY	1 cut(s)	1363
BstYI	RGATCY	1 cut(s)	1363
BstZI	CGGCCG	1 cut(s)	1422
BsuRI	GGCC	4 cut(s)	5, 319, 1370, 1424
BtrI	CACGTC	1 cut(s)	827
BtsCI	GGATG	5 cut(s)	550, 567, 946, 1223, 1438
BtsI	GCAGTG	1 cut(s)	865
BtsIMutI	CAGTG	2 cut(s)	865, 1008
Cac8I	GCNNGC	3 cut(s)	54, 58, 1243
CaiI	CAGNNNCTG	2 cut(s)	659, 1010
CciNI	GCGGCCGC	1 cut(s)	1422
CfoI	GCGC	1 cut(s)	338
Cfr13I	GGNCC	1 cut(s)	193
CseI	GACGC	2 cut(s)	864, 875
CspCI	CAANNNNNGTGG	2 cut(s)	1360, 1395
CviAII	CATG	6 cut(s)	57, 223, 666, 778, 817, 917
DdeI	CTNAG	7 cut(s)	48, 116, 805, 958, 1400, 1433, 1490
DinI	GGCGCC	1 cut(s)	337
DpnI	GATC	6 cut(s)	425, 869, 983, 1173, 1341, 1365
DpnII	GATC	6 cut(s)	423, 867, 981, 1171, 1339, 1363
EaeI	YGGCCR	2 cut(s)	1368, 1422
EagI	CGGCCG	1 cut(s)	1422
Eam1104I	CTCTTC	2 cut(s)	85, 240
EarI	CTCTTC	2 cut(s)	85, 240
EclXI	CGGCCG	1 cut(s)	1422
Eco24I	GRGCYC	1 cut(s)	535
Eco31I	GGTCTC	1 cut(s)	1312
Eco47I	GGWCC	1 cut(s)	193
Eco52I	CGGCCG	1 cut(s)	1422
Eco57I	CTGAAG	3 cut(s)	593, 624, 1221
Eco88I	CYCGRG	1 cut(s)	1118
Eco91I	GGTNACC	1 cut(s)	1460
EcoNI	CCTNNNNNAGG	1 cut(s)	575
EcoO65I	GGTNACC	1 cut(s)	1460
EcoRI	GAATTC	1 cut(s)	617
EcoRII	CCWGG	1 cut(s)	1464
EcoT22I	ATGCAT	2 cut(s)	145, 349
EcoT38I	GRGCYC	1 cut(s)	535
EgeI	GGCGCC	1 cut(s)	337
EheI	GGCGCC	1 cut(s)	337
FaeI	CATG	6 cut(s)	60, 226, 669, 781, 820, 920
FaqI	GGGAC	1 cut(s)	990
FatI	CATG	6 cut(s)	56, 222, 665, 777, 816, 916
FbaI	TGATCA	1 cut(s)	867
FblI	GTMKAC	1 cut(s)	843
FokI	GGATG	5 cut(s)	554, 557, 953, 1210, 1425
FriOI	GRGCYC	1 cut(s)	535
FspBI	CTAG	2 cut(s)	648, 1055
GlaI	GCGC	1 cut(s)	337
GsaI	CCCAGC	2 cut(s)	291, 1363
HaeII	RGCGCY	1 cut(s)	339
HaeIII	GGCC	4 cut(s)	5, 319, 1370, 1424
HapII	CCGG	4 cut(s)	320, 468, 1018, 1145
HgaI	GACGC	2 cut(s)	864, 875
HhaI	GCGC	1 cut(s)	338
Hin1I	GRCGYC	1 cut(s)	336
Hin1II	CATG	6 cut(s)	60, 226, 669, 781, 820, 920
Hin6I	GCGC	1 cut(s)	336
HinP1I	GCGC	1 cut(s)	336
HindIII	AAGCTT	1 cut(s)	1376
HinfI	GANTC	5 cut(s)	120, 325, 809, 845, 1346
HpaII	CCGG	4 cut(s)	320, 468, 1018, 1145
HphI	GGTGA	2 cut(s)	592, 1472
Hpy166II	GTNNAC	3 cut(s)	38, 717, 844
Hpy188I	TCNGA	7 cut(s)	119, 256, 483, 854, 1264, 1387, 1434
Hpy188III	TCNNGA	9 cut(s)	571, 648, 938, 1018, 1076, 1118, 1169, 1337, 1343
Hpy8I	GTNNAC	3 cut(s)	38, 717, 844
HpyAV	CCTTC	8 cut(s)	78, 362, 437, 556, 571, 967, 1294, 1398
HpyCH4III	ACNGT	4 cut(s)	508, 540, 714, 1166
HpyCH4IV	ACGT	2 cut(s)	34, 826
HpyF10VI	GCNNNNNNNGC	3 cut(s)	344, 883, 1427
HpyF3I	CTNAG	7 cut(s)	48, 116, 805, 958, 1400, 1433, 1490
HpySE526I	ACGT	2 cut(s)	34, 826
Hsp92I	GRCGYC	1 cut(s)	336
Hsp92II	CATG	6 cut(s)	60, 226, 669, 781, 820, 920
HspAI	GCGC	1 cut(s)	336
KasI	GGCGCC	1 cut(s)	335
Kpn2I	TCCGGA	1 cut(s)	1017
Ksp22I	TGATCA	1 cut(s)	867
Kzo9I	GATC	6 cut(s)	423, 867, 981, 1171, 1339, 1363
LguI	GCTCTTC	1 cut(s)	240
LmnI	GCTCC	3 cut(s)	364, 538, 1095
Lsp1109I	GCAGC	9 cut(s)	86, 658, 665, 1016, 1117, 1168, 1414, 1459, 1481
LweI	GCATC	3 cut(s)	125, 334, 1447
MaeI	CTAG	2 cut(s)	648, 1055
MaeII	ACGT	2 cut(s)	34, 826
MaeIII	GTNAC	4 cut(s)	708, 964, 1329, 1460
MalI	GATC	6 cut(s)	425, 869, 983, 1173, 1341, 1365
MboI	GATC	6 cut(s)	423, 867, 981, 1171, 1339, 1363
MfeI	CAATTG	1 cut(s)	1211
MflI	RGATCY	1 cut(s)	1363
MhlI	GDGCHC	2 cut(s)	535, 1092
MlsI	TGGCCA	1 cut(s)	1370
MluNI	TGGCCA	1 cut(s)	1370
Mly113I	GGCGCC	1 cut(s)	336
MlyI	GAGTC	2 cut(s)	818, 839
MmeI	TCCRAC	2 cut(s)	357, 522
Mox20I	TGGCCA	1 cut(s)	1370
Mph1103I	ATGCAT	2 cut(s)	145, 349
MroI	TCCGGA	1 cut(s)	1017
MscI	TGGCCA	1 cut(s)	1370
MseI	TTAA	6 cut(s)	555, 801, 906, 1229, 1247, 1278
MslI	CAYNNNNRTG	2 cut(s)	265, 939
Msp20I	TGGCCA	1 cut(s)	1370
MspA1I	CMGCKG	3 cut(s)	1421, 1427, 1472
MspCI	CTTAAG	2 cut(s)	905, 1228
MspI	CCGG	4 cut(s)	320, 468, 1018, 1145
MspR9I	CCNGG	3 cut(s)	321, 469, 1466
MunI	CAATTG	1 cut(s)	1211
Mva1269I	GAATGC	1 cut(s)	143
MvaI	CCWGG	1 cut(s)	1466
MwoI	GCNNNNNNNGC	3 cut(s)	344, 883, 1427
NarI	GGCGCC	1 cut(s)	336
NciI	CCSGG	2 cut(s)	321, 469
NdeII	GATC	6 cut(s)	423, 867, 981, 1171, 1339, 1363
NlaIII	CATG	6 cut(s)	60, 226, 669, 781, 820, 920
NlaIV	GGNNCC	2 cut(s)	337, 534
NmuCI	GTSAC	4 cut(s)	708, 964, 1329, 1460
NotI	GCGGCCGC	1 cut(s)	1422
NsiI	ATGCAT	2 cut(s)	145, 349
NspI	RCATGY	2 cut(s)	60, 669
PaeI	GCATGC	1 cut(s)	60
PciI	ACATGT	1 cut(s)	665
PciSI	GCTCTTC	1 cut(s)	240
PctI	GAATGC	1 cut(s)	143
PfeI	GAWTC	3 cut(s)	120, 325, 1346
PflFI	GACNNNGTC	1 cut(s)	848
PleI	GAGTC	2 cut(s)	817, 839
PluTI	GGCGCC	1 cut(s)	339
PpsI	GAGTC	2 cut(s)	817, 839
Ppu21I	YACGTR	1 cut(s)	35
PscI	ACATGT	1 cut(s)	665
PshAI	GACNNNNGTC	1 cut(s)	1068
Psp6I	CCWGG	1 cut(s)	1464
PspEI	GGTNACC	1 cut(s)	1460
PspFI	CCCAGC	2 cut(s)	287, 1359
PspGI	CCWGG	1 cut(s)	1464
PspN4I	GGNNCC	2 cut(s)	337, 534
PspPI	GGNCC	1 cut(s)	193
PstI	CTGCAG	2 cut(s)	369, 1477
PstNI	CAGNNNCTG	2 cut(s)	659, 1010
PsuI	RGATCY	1 cut(s)	1363
PsyI	GACNNNGTC	1 cut(s)	848
PvuII	CAGCTG	1 cut(s)	1472
RseI	CAYNNNNRTG	2 cut(s)	265, 939
SapI	GCTCTTC	1 cut(s)	240
SaqAI	TTAA	6 cut(s)	555, 801, 906, 1229, 1247, 1278
Sau3AI	GATC	6 cut(s)	423, 867, 981, 1171, 1339, 1363
Sau96I	GGNCC	1 cut(s)	193
SchI	GAGTC	2 cut(s)	818, 839
ScrFI	CCNGG	3 cut(s)	321, 469, 1466
SduI	GDGCHC	2 cut(s)	535, 1092
SfaNI	GCATC	3 cut(s)	125, 334, 1447
SfcI	CTRYAG	2 cut(s)	365, 1473
SfoI	GGCGCC	1 cut(s)	337
SinI	GGWCC	1 cut(s)	193
SmiMI	CAYNNNNRTG	2 cut(s)	265, 939
SmlI	CTYRAG	2 cut(s)	905, 1228
SmoI	CTYRAG	2 cut(s)	905, 1228
SphI	GCATGC	1 cut(s)	60
SsiI	CCGC	3 cut(s)	312, 1421, 1425
SspDI	GGCGCC	1 cut(s)	335
SspMI	CTAG	2 cut(s)	648, 1055
StyD4I	CCNGG	3 cut(s)	319, 467, 1464
TaaI	ACNGT	4 cut(s)	508, 540, 714, 1166
TaiI	ACGT	2 cut(s)	37, 829
TaqI	TCGA	4 cut(s)	498, 621, 750, 1344
TauI	GCSGC	2 cut(s)	1424, 1427
TfiI	GAWTC	3 cut(s)	120, 325, 1346
Tru1I	TTAA	6 cut(s)	555, 801, 906, 1229, 1247, 1278
Tru9I	TTAA	6 cut(s)	555, 801, 906, 1229, 1247, 1278
TscAI	CASTG	2 cut(s)	865, 1015
TseFI	GTSAC	4 cut(s)	708, 964, 1329, 1460
TseI	GCWGC	9 cut(s)	99, 653, 671, 1004, 1130, 1156, 1427, 1469, 1472
Tsp45I	GTSAC	4 cut(s)	708, 964, 1329, 1460
TspDTI	ATGAA	3 cut(s)	211, 794, 923
TspGWI	ACGGA	1 cut(s)	838
TspRI	CASTG	2 cut(s)	865, 1015
Tth111I	GACNNNGTC	1 cut(s)	848
Vha464I	CTTAAG	2 cut(s)	905, 1228
VpaK11BI	GGWCC	1 cut(s)	193
XagI	CCTNNNNNAGG	1 cut(s)	575
XapI	RAATTY	4 cut(s)	617, 746, 791, 1252
XbaI	TCTAGA	1 cut(s)	647
XceI	RCATGY	2 cut(s)	60, 669
XmiI	GTMKAC	1 cut(s)	843
XspI	CTAG	2 cut(s)	648, 1055
Zsp2I	ATGCAT	2 cut(s)	145, 349

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.