Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0013246

Physical Location & Seq

Genomic Coordinates Forward (+)

27322948 .. 27325543

Gene Structure 2596 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1190500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1671 bp

ATGTTTTCTCTTATTTTCCATATTTTTTGTGCTTTTTGGGGAAGCAAATTTCACATGTGGATAAATCAGAGGACACGTGTCAAAGTGCCAGCCTTTGCTAACACTTCTAGTAGTTCTCCCACAGAACAAAAAGTTCACACTTCACAACATAACCTCCATACAAGCATGGCCTATCTGCACTCTCCAAGTCTAACCACACTTAAACACAACCCAAAACCTGCATGCTTCCCTCCTTCAAAACCCCCTCCACTTCACAATCTCTGCTTCTTCACAACCCAACTCAAAATCCAGCCTCAGAGATGCAATGCATTCTTCGGCGAAATCTCCGATAGTATTTTGGAAACCGCCCTCCATTTGGACCAATTTCCTCCTCTGCAATATGGTTTCATGCAGTTTGAGAGGATCACAGAGGAACTATCAGAAACACAGAAGTGGGGGTTCCTTGTTTTTGCTGGGGTTACTTGGATATACTTAACCGCGAGGCCGGGGATTCTCATTGGCGCCATTGATGCGTACCTTCTTGCTCCTCTGCAGTTGGTGTTGGACAGCTTGATTGGGAGGAGGAGGTTGAAGAGGACGGACTTTGTGATCGGGGAAAAATTGGGAGAGGGGTCTTTTGGTGTGGTATATTCCGGGGCAGTTGTTCCGAAGAATGTGAATATGGAGGAGACGGTGCAGAGGAGAGGAAGGGGCAGGGCACCACAATTGGATGAGAGGTTTAAGGAGAAGGTCATCCTCAAGAAGGTGAAGATTGAAGTTCAAGGGGCTGAAGAATTTGGCGAATTCGAGGAGTGGTTTAATTACAGATTGTCTAGAGCAGCACCTGAAACATGTGCTGCGTTCCTGGGAAGTTTTGTTGCTGATAAAACAACATCACAGTTTACAAAGGGTGGAAAGTGGCTCGTCTGGAAATTCGAGGGAAACCAAAACCTTGATGATTACATGAAAGATCGGAAATTCCCTTTTAACTTGGAGTCTGTCATGTTTGGACGTGTCCTGCAAGATGTAGACTCCGTCAAACGCAGTGCATTGATCATCAAGCAAATAATGCGTCAAATTATTACTTCGCTCAAGAAAATCCATGACACGGGCATTGTCCATCGGGATGTAAAGCCAGCGAACTTAGTAGTAACAAAAAAGGGACAGATCAAGCTCATAGATTTCGGGGCAGCCACTGACCTCCGGATTGGAAAGAATTATATACCAGACCGTGCCCTTCTTGACCCTAGCTATTGTCCTCCTGAACTATATGTGCTCCCAGAGGAAACACCGAATCCTCCTCCTGAGCCTATTGCCGCCTTACTTTCTCCGATTCTTTGGCAGCTGAACAGTCCTGATCTGTTTGATATGTATTCTGCGGGAATTATACTACTGCAAATGGCAATACCATCCTTAAGGTCTTCAGCAGGCTTAAAGAATTTCAATTCAGAGATAAAAATGGTTAAATACGATTTGAATAAATGGAGGGACTACACTCGGTTGAGGCCTGACTTGTCACTTCTTGATCTTGATTCGGGTAGAGGCTGGGATCTGGCCACAAAGCTTGTTTCCGAGAGAGGTTCCCTTAGAAGAGGGCGCTTATCTGCCGCTGCTGCTCTAAGGCATCCTTATTTCTTGCTAGCTGGTGACCAGGCAGCTGCAGTTCTCTCAAAATTAAGTTTTACCAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009579 GO:0009987 GO:0010109 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0031323 GO:0036211 GO:0042548 GO:0042549 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043467 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

557

Amino Acids

62.97

Weight (kDa)

9.41

Isoelectric Point (pI)

43.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	197 - 416	8.2e-11	Protein tyrosine and serine/threonine kinase
ABC1	PF03109	330 - 394	8.3e-08	ABC1 atypical kinase-like domain
Pkinase	PF00069	340 - 416	4.4e-18	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0013246)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01920 AT5G01920
fragaria_vesca	FvH4_6g09630
malus_domestica	MD04G1176200.v1.1 MD12G1190500.v1.1
prunus_persica	Prupe.6G295600_v2.0.a1
pyrus_communis	pycom04g15540
rosa_chinensis	RchiOBHm_Chr3g0460271
rosa_laevigata	RLG00000025029
rosa_multiflora	Rmu_co8344035.1_g000001 Rmu_sc0003763.1_g000001 Rmu_sc0035433.1_g000001
rosa_rugosa	Rorug03G0041200
rosa_samantha	Rh3AG099100 Rh3BG103000 Rh3CG104600 Rh3DG104000
rosa_wichuraiana	Rw3G008400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	226
AccB1I	GGYRCC	2 cut(s)	500, 697
AccI	GTMKAC	1 cut(s)	1008
AccII	CGCG	1 cut(s)	479
AccIII	TCCGGA	1 cut(s)	1182
AciI	CCGC	5 cut(s)	345, 477, 1296, 1358, 1587
AclWI	GGATC	2 cut(s)	410, 1536
AcoI	YGGCCR	1 cut(s)	1533
AcsI	RAATTY	6 cut(s)	47, 773, 782, 911, 956, 1417
AcuI	CTGAAG	2 cut(s)	789, 1386
AcvI	CACGTG	1 cut(s)	77
AcyI	GRCGYC	1 cut(s)	501
AfaI	GTAC	1 cut(s)	515
AfiI	CCNNNNNNNGG	3 cut(s)	355, 742, 1087
AflII	CTTAAG	1 cut(s)	1393
AflIII	ACRYGT	5 cut(s)	54, 74, 76, 830, 991
AgsI	TTSAA	6 cut(s)	237, 571, 755, 761, 1423, 1456
AjiI	CACGTC	1 cut(s)	992
AjnI	CCWGG	2 cut(s)	843, 1629
AleI	CACNNNNGTG	1 cut(s)	430
AluBI	AGCT	7 cut(s)	549, 1153, 1230, 1324, 1543, 1622, 1637
AluI	AGCT	7 cut(s)	549, 1153, 1230, 1324, 1543, 1622, 1637
Alw21I	GWGCWC	1 cut(s)	1257
Alw26I	GTCTC	1 cut(s)	662
AlwI	GGATC	2 cut(s)	410, 1536
AlwNI	CAGNNNCTG	2 cut(s)	824, 1175
Aor13HI	TCCGGA	1 cut(s)	1182
AoxI	GGCC	4 cut(s)	168, 482, 1484, 1533
ApeKI	GCWGC	8 cut(s)	818, 836, 1169, 1321, 1589, 1592, 1634, 1637
ApoI	RAATTY	6 cut(s)	47, 773, 782, 911, 956, 1417
ArsI	GACNNNNNNTTYG	2 cut(s)	1036, 1068
AspLEI	GCGC	2 cut(s)	503, 1578
AspS9I	GGNCC	1 cut(s)	358
AsuC2I	CCSGG	2 cut(s)	486, 634
AsuHPI	GGTGA	2 cut(s)	757, 1637
AsuNHI	GCTAGC	1 cut(s)	1618
AvaII	GGWCC	1 cut(s)	358
BaeGI	GKGCMC	2 cut(s)	700, 1216
BalI	TGGCCA	1 cut(s)	1535
BanI	GGYRCC	2 cut(s)	500, 697
BbrPI	CACGTG	1 cut(s)	77
BbsI	GAAGAC	1 cut(s)	1392
Bbv12I	GWGCWC	1 cut(s)	1257
BbvI	GCAGC	8 cut(s)	823, 830, 1181, 1333, 1576, 1579, 1624, 1646
BccI	CCATC	2 cut(s)	1107, 1396
BciT130I	CCWGG	2 cut(s)	845, 1631
BclI	TGATCA	1 cut(s)	1032
BcnI	CCSGG	2 cut(s)	486, 634
BcoDI	GTCTC	1 cut(s)	662
BfaI	CTAG	4 cut(s)	108, 813, 1227, 1619
BfmI	CTRYAG	2 cut(s)	530, 1638
BfoI	RGCGCY	2 cut(s)	504, 1579
BfrI	CTTAAG	1 cut(s)	1393
BfuAI	ACCTGC	1 cut(s)	226
Bme1390I	CCNGG	4 cut(s)	486, 634, 845, 1631
Bme18I	GGWCC	1 cut(s)	358
BmgBI	CACGTC	1 cut(s)	992
BmgT120I	GGNCC	1 cut(s)	358
BmiI	GGNNCC	4 cut(s)	440, 502, 699, 1561
BmrFI	CCNGG	4 cut(s)	486, 634, 845, 1631
BmsI	GCATC	3 cut(s)	290, 499, 1612
BmtI	GCTAGC	1 cut(s)	1622
BoxI	GACNNNNGTC	1 cut(s)	77
BpiI	GAAGAC	1 cut(s)	1392
Bpu10I	CCTNAGC	1 cut(s)	1284
BpuEI	CTTGAG	2 cut(s)	722, 1055
BpuMI	CCSGG	2 cut(s)	486, 634
BsaAI	YACGTR	1 cut(s)	77
BsaHI	GRCGYC	1 cut(s)	501
BsaJI	CCNNGG	3 cut(s)	485, 633, 844
BsaWI	WCCGGW	1 cut(s)	1182
BsaXI	ACNNNNNCTCC	2 cut(s)	138, 168
Bsc4I	CCNNNNNNNGG	3 cut(s)	355, 742, 1087
Bse3DI	GCAATG	1 cut(s)	310
BseAI	TCCGGA	1 cut(s)	1182
BseBI	CCWGG	2 cut(s)	845, 1631
BseDI	CCNNGG	3 cut(s)	485, 633, 844
BseGI	GGATG	5 cut(s)	715, 732, 1111, 1388, 1603
BseLI	CCNNNNNNNGG	3 cut(s)	355, 742, 1087
BseMI	GCAATG	1 cut(s)	310
BseMII	CTCAG	2 cut(s)	308, 1275
BseRI	GAGGAG	8 cut(s)	360, 516, 574, 577, 680, 694, 803, 1269
BseSI	GKGCMC	2 cut(s)	700, 1216
BseXI	GCAGC	8 cut(s)	823, 830, 1181, 1333, 1576, 1579, 1624, 1646
BseYI	CCCAGC	2 cut(s)	452, 1524
BsgI	GTGCAG	2 cut(s)	161, 695
Bsh1236I	CGCG	1 cut(s)	479
BshFI	GGCC	4 cut(s)	170, 484, 1486, 1535
BshNI	GGYRCC	2 cut(s)	500, 697
BsiHKAI	GWGCWC	1 cut(s)	1257
BsiSI	CCGG	3 cut(s)	485, 633, 1183
BslFI	GGGAC	2 cut(s)	1155, 1481
BslI	CCNNNNNNNGG	3 cut(s)	355, 742, 1087
BsmAI	GTCTC	1 cut(s)	662
BsmBI	CGTCTC	1 cut(s)	662
BsmFI	GGGAC	2 cut(s)	1155, 1481
BsmI	GAATGC	1 cut(s)	308
BsnI	GGCC	4 cut(s)	170, 484, 1486, 1535
Bsp1286I	GDGCHC	3 cut(s)	700, 1216, 1257
Bsp13I	TCCGGA	1 cut(s)	1182
Bsp143I	GATC	8 cut(s)	402, 588, 949, 1032, 1146, 1336, 1504, 1528
BspACI	CCGC	5 cut(s)	345, 477, 1296, 1358, 1587
BspANI	GGCC	4 cut(s)	170, 484, 1486, 1535
BspCNI	CTCAG	2 cut(s)	307, 1276
BspEI	TCCGGA	1 cut(s)	1182
BspFNI	CGCG	1 cut(s)	479
BspLI	GGNNCC	4 cut(s)	440, 502, 699, 1561
BspMAI	CTGCAG	2 cut(s)	534, 1642
BspMI	ACCTGC	1 cut(s)	226
BspOI	GCTAGC	1 cut(s)	1622
BspPI	GGATC	2 cut(s)	410, 1536
BspT107I	GGYRCC	2 cut(s)	500, 697
BspTI	CTTAAG	1 cut(s)	1393
BsrDI	GCAATG	1 cut(s)	310
BssECI	CCNNGG	3 cut(s)	485, 633, 844
BssMI	GATC	8 cut(s)	402, 588, 949, 1032, 1146, 1336, 1504, 1528
BssNI	GRCGYC	1 cut(s)	501
Bst2UI	CCWGG	2 cut(s)	845, 1631
Bst4CI	ACNGT	4 cut(s)	673, 879, 1211, 1331
Bst6I	CTCTTC	2 cut(s)	566, 1564
BstACI	GRCGYC	1 cut(s)	501
BstAFI	CTTAAG	1 cut(s)	1393
BstAPI	GCANNNNNTGC	1 cut(s)	1048
BstBAI	YACGTR	1 cut(s)	77
BstC8I	GCNNGC	5 cut(s)	90, 223, 1116, 1408, 1620
BstDEI	CTNAG	5 cut(s)	294, 1123, 1284, 1565, 1598
BstEII	GGTNACC	1 cut(s)	1625
BstENI	CCTNNNNNAGG	1 cut(s)	740
BstF5I	GGATG	5 cut(s)	715, 732, 1111, 1388, 1603
BstFNI	CGCG	1 cut(s)	479
BstH2I	RGCGCY	2 cut(s)	504, 1579
BstHHI	GCGC	2 cut(s)	503, 1578
BstKTI	GATC	8 cut(s)	405, 591, 952, 1035, 1149, 1339, 1507, 1531
BstMAI	GTCTC	1 cut(s)	662
BstMBI	GATC	8 cut(s)	402, 588, 949, 1032, 1146, 1336, 1504, 1528
BstMWI	GCNNNNNNNGC	3 cut(s)	509, 1048, 1592
BstNI	CCWGG	2 cut(s)	845, 1631
BstNSI	RCATGY	3 cut(s)	58, 225, 834
BstPAI	GACNNNNGTC	1 cut(s)	77
BstPI	GGTNACC	1 cut(s)	1625
BstSCI	CCNGG	4 cut(s)	484, 632, 843, 1629
BstSFI	CTRYAG	2 cut(s)	530, 1638
BstSLI	GKGCMC	2 cut(s)	700, 1216
BstUI	CGCG	1 cut(s)	479
BstV1I	GCAGC	8 cut(s)	823, 830, 1181, 1333, 1576, 1579, 1624, 1646
BstV2I	GAAGAC	1 cut(s)	1392
BstX2I	RGATCY	1 cut(s)	1528
BstYI	RGATCY	1 cut(s)	1528
BsuRI	GGCC	4 cut(s)	170, 484, 1486, 1535
BtrI	CACGTC	1 cut(s)	992
BtsCI	GGATG	5 cut(s)	715, 732, 1111, 1388, 1603
BtsI	GCAGTG	1 cut(s)	1030
BtsIMutI	CAGTG	2 cut(s)	1030, 1173
BveI	ACCTGC	1 cut(s)	226
Cac8I	GCNNGC	5 cut(s)	90, 223, 1116, 1408, 1620
CaiI	CAGNNNCTG	2 cut(s)	824, 1175
CfoI	GCGC	2 cut(s)	503, 1578
Cfr13I	GGNCC	1 cut(s)	358
CseI	GACGC	1 cut(s)	1040
Csp6I	GTAC	1 cut(s)	514
CspCI	CAANNNNNGTGG	2 cut(s)	1525, 1560
CviAII	CATG	8 cut(s)	55, 166, 222, 388, 831, 943, 982, 1082
CviQI	GTAC	1 cut(s)	514
DdeI	CTNAG	5 cut(s)	294, 1123, 1284, 1565, 1598
DinI	GGCGCC	1 cut(s)	502
DpnI	GATC	8 cut(s)	404, 590, 951, 1034, 1148, 1338, 1506, 1530
DpnII	GATC	8 cut(s)	402, 588, 949, 1032, 1146, 1336, 1504, 1528
EaeI	YGGCCR	1 cut(s)	1533
Eam1104I	CTCTTC	2 cut(s)	566, 1564
EarI	CTCTTC	2 cut(s)	566, 1564
Eco147I	AGGCCT	1 cut(s)	1486
Eco47I	GGWCC	1 cut(s)	358
Eco57I	CTGAAG	2 cut(s)	789, 1386
Eco72I	CACGTG	1 cut(s)	77
Eco91I	GGTNACC	1 cut(s)	1625
EcoNI	CCTNNNNNAGG	1 cut(s)	740
EcoO65I	GGTNACC	1 cut(s)	1625
EcoRI	GAATTC	1 cut(s)	782
EcoRII	CCWGG	2 cut(s)	843, 1629
EcoT22I	ATGCAT	1 cut(s)	310
EgeI	GGCGCC	1 cut(s)	502
EheI	GGCGCC	1 cut(s)	502
Esp3I	CGTCTC	1 cut(s)	662
FaeI	CATG	8 cut(s)	58, 169, 225, 391, 834, 946, 985, 1085
FalI	AAGNNNNNCTT	2 cut(s)	1591, 1623
FaqI	GGGAC	2 cut(s)	1155, 1481
FatI	CATG	8 cut(s)	54, 165, 221, 387, 830, 942, 981, 1081
FauI	CCCGC	1 cut(s)	1351
FbaI	TGATCA	1 cut(s)	1032
FblI	GTMKAC	1 cut(s)	1008
FokI	GGATG	5 cut(s)	719, 722, 1118, 1375, 1590
FspBI	CTAG	4 cut(s)	108, 813, 1227, 1619
GlaI	GCGC	2 cut(s)	502, 1577
GsaI	CCCAGC	2 cut(s)	456, 1528
HaeII	RGCGCY	2 cut(s)	504, 1579
HaeIII	GGCC	4 cut(s)	170, 484, 1486, 1535
HapII	CCGG	3 cut(s)	485, 633, 1183
HgaI	GACGC	1 cut(s)	1040
HhaI	GCGC	2 cut(s)	503, 1578
Hin1I	GRCGYC	1 cut(s)	501
Hin1II	CATG	8 cut(s)	58, 169, 225, 391, 834, 946, 985, 1085
Hin6I	GCGC	2 cut(s)	501, 1576
HinP1I	GCGC	2 cut(s)	501, 1576
HindIII	AAGCTT	1 cut(s)	1541
HinfI	GANTC	6 cut(s)	490, 974, 1010, 1273, 1312, 1511
HpaII	CCGG	3 cut(s)	485, 633, 1183
HphI	GGTGA	2 cut(s)	757, 1637
Hpy166II	GTNNAC	3 cut(s)	136, 882, 1009
Hpy188I	TCNGA	9 cut(s)	69, 297, 328, 421, 648, 954, 1311, 1429, 1552
Hpy8I	GTNNAC	3 cut(s)	136, 882, 1009
HpyAV	CCTTC	6 cut(s)	243, 527, 681, 721, 736, 1226
HpyCH4III	ACNGT	4 cut(s)	673, 879, 1211, 1331
HpyCH4IV	ACGT	2 cut(s)	76, 991
HpyF10VI	GCNNNNNNNGC	3 cut(s)	509, 1048, 1592
HpyF3I	CTNAG	5 cut(s)	294, 1123, 1284, 1565, 1598
HpySE526I	ACGT	2 cut(s)	76, 991
Hsp92I	GRCGYC	1 cut(s)	501
Hsp92II	CATG	8 cut(s)	58, 169, 225, 391, 834, 946, 985, 1085
HspAI	GCGC	2 cut(s)	501, 1576
KasI	GGCGCC	1 cut(s)	500
Kpn2I	TCCGGA	1 cut(s)	1182
Ksp22I	TGATCA	1 cut(s)	1032
Kzo9I	GATC	8 cut(s)	402, 588, 949, 1032, 1146, 1336, 1504, 1528
LmnI	GCTCC	2 cut(s)	529, 1260
Lsp1109I	GCAGC	8 cut(s)	823, 830, 1181, 1333, 1576, 1579, 1624, 1646
LweI	GCATC	3 cut(s)	290, 499, 1612
MaeI	CTAG	4 cut(s)	108, 813, 1227, 1619
MaeII	ACGT	2 cut(s)	76, 991
MaeIII	GTNAC	4 cut(s)	457, 1129, 1494, 1625
MalI	GATC	8 cut(s)	404, 590, 951, 1034, 1148, 1338, 1506, 1530
MboI	GATC	8 cut(s)	402, 588, 949, 1032, 1146, 1336, 1504, 1528
MboII	GAAGA	8 cut(s)	259, 304, 583, 661, 760, 782, 1392, 1581
MfeI	CAATTG	1 cut(s)	704
MflI	RGATCY	1 cut(s)	1528
MhlI	GDGCHC	3 cut(s)	700, 1216, 1257
MlsI	TGGCCA	1 cut(s)	1535
MluNI	TGGCCA	1 cut(s)	1535
Mly113I	GGCGCC	1 cut(s)	501
MlyI	GAGTC	2 cut(s)	983, 1004
MmeI	TCCRAC	1 cut(s)	522
Mox20I	TGGCCA	1 cut(s)	1535
Mph1103I	ATGCAT	1 cut(s)	310
MroI	TCCGGA	1 cut(s)	1182
MscI	TGGCCA	1 cut(s)	1535
MseI	TTAA	9 cut(s)	201, 473, 720, 798, 966, 1394, 1412, 1443, 1655
MslI	CAYNNNNRTG	2 cut(s)	430, 1104
Msp20I	TGGCCA	1 cut(s)	1535
MspA1I	CMGCKG	3 cut(s)	1324, 1589, 1637
MspCI	CTTAAG	1 cut(s)	1393
MspI	CCGG	3 cut(s)	485, 633, 1183
MspR9I	CCNGG	4 cut(s)	486, 634, 845, 1631
MunI	CAATTG	1 cut(s)	704
Mva1269I	GAATGC	1 cut(s)	308
MvaI	CCWGG	2 cut(s)	845, 1631
MvnI	CGCG	1 cut(s)	479
MwoI	GCNNNNNNNGC	3 cut(s)	509, 1048, 1592
NarI	GGCGCC	1 cut(s)	501
NciI	CCSGG	2 cut(s)	486, 634
NdeII	GATC	8 cut(s)	402, 588, 949, 1032, 1146, 1336, 1504, 1528
NheI	GCTAGC	1 cut(s)	1618
NlaIII	CATG	8 cut(s)	58, 169, 225, 391, 834, 946, 985, 1085
NlaIV	GGNNCC	4 cut(s)	440, 502, 699, 1561
NmuCI	GTSAC	2 cut(s)	1494, 1625
NsiI	ATGCAT	1 cut(s)	310
NspI	RCATGY	3 cut(s)	58, 225, 834
OliI	CACNNNNGTG	1 cut(s)	430
PaeI	GCATGC	1 cut(s)	225
PceI	AGGCCT	1 cut(s)	1486
PciI	ACATGT	2 cut(s)	54, 830
PctI	GAATGC	1 cut(s)	308
PfeI	GAWTC	4 cut(s)	490, 1273, 1312, 1511
PflFI	GACNNNGTC	1 cut(s)	1013
PleI	GAGTC	2 cut(s)	982, 1004
PluTI	GGCGCC	1 cut(s)	504
PmaCI	CACGTG	1 cut(s)	77
PmlI	CACGTG	1 cut(s)	77
PpsI	GAGTC	2 cut(s)	982, 1004
Ppu21I	YACGTR	1 cut(s)	77
PscI	ACATGT	2 cut(s)	54, 830
PshAI	GACNNNNGTC	1 cut(s)	77
Psp6I	CCWGG	2 cut(s)	843, 1629
PspCI	CACGTG	1 cut(s)	77
PspEI	GGTNACC	1 cut(s)	1625
PspFI	CCCAGC	2 cut(s)	452, 1524
PspGI	CCWGG	2 cut(s)	843, 1629
PspN4I	GGNNCC	4 cut(s)	440, 502, 699, 1561
PspPI	GGNCC	1 cut(s)	358
PstI	CTGCAG	2 cut(s)	534, 1642
PstNI	CAGNNNCTG	2 cut(s)	824, 1175
PsuI	RGATCY	1 cut(s)	1528
PsyI	GACNNNGTC	1 cut(s)	1013
PvuII	CAGCTG	2 cut(s)	1324, 1637
RsaI	GTAC	1 cut(s)	515
RsaNI	GTAC	1 cut(s)	514
RseI	CAYNNNNRTG	2 cut(s)	430, 1104
SaqAI	TTAA	9 cut(s)	201, 473, 720, 798, 966, 1394, 1412, 1443, 1655
Sau3AI	GATC	8 cut(s)	402, 588, 949, 1032, 1146, 1336, 1504, 1528
Sau96I	GGNCC	1 cut(s)	358
SchI	GAGTC	2 cut(s)	983, 1004
ScrFI	CCNGG	4 cut(s)	486, 634, 845, 1631
SduI	GDGCHC	3 cut(s)	700, 1216, 1257
SfaNI	GCATC	3 cut(s)	290, 499, 1612
SfcI	CTRYAG	2 cut(s)	530, 1638
SfoI	GGCGCC	1 cut(s)	502
SinI	GGWCC	1 cut(s)	358
SmiMI	CAYNNNNRTG	2 cut(s)	430, 1104
SmlI	CTYRAG	3 cut(s)	737, 1070, 1393
SmoI	CTYRAG	3 cut(s)	737, 1070, 1393
SphI	GCATGC	1 cut(s)	225
SseBI	AGGCCT	1 cut(s)	1486
SsiI	CCGC	5 cut(s)	345, 477, 1296, 1358, 1587
SspDI	GGCGCC	1 cut(s)	500
SspMI	CTAG	4 cut(s)	108, 813, 1227, 1619
StuI	AGGCCT	1 cut(s)	1486
StyD4I	CCNGG	4 cut(s)	484, 632, 843, 1629
TaaI	ACNGT	4 cut(s)	673, 879, 1211, 1331
TaiI	ACGT	2 cut(s)	79, 994
TaqI	TCGA	2 cut(s)	786, 915
TauI	GCSGC	2 cut(s)	1298, 1589
TfiI	GAWTC	4 cut(s)	490, 1273, 1312, 1511
Tru1I	TTAA	9 cut(s)	201, 473, 720, 798, 966, 1394, 1412, 1443, 1655
Tru9I	TTAA	9 cut(s)	201, 473, 720, 798, 966, 1394, 1412, 1443, 1655
TscAI	CASTG	2 cut(s)	1030, 1180
TseFI	GTSAC	2 cut(s)	1494, 1625
TseI	GCWGC	8 cut(s)	818, 836, 1169, 1321, 1589, 1592, 1634, 1637
Tsp45I	GTSAC	2 cut(s)	1494, 1625
TspDTI	ATGAA	2 cut(s)	376, 959
TspGWI	ACGGA	2 cut(s)	593, 1003
TspRI	CASTG	2 cut(s)	1030, 1180
Tth111I	GACNNNGTC	1 cut(s)	1013
Vha464I	CTTAAG	1 cut(s)	1393
VpaK11BI	GGWCC	1 cut(s)	358
XagI	CCTNNNNNAGG	1 cut(s)	740
XapI	RAATTY	6 cut(s)	47, 773, 782, 911, 956, 1417
XbaI	TCTAGA	1 cut(s)	812
XceI	RCATGY	3 cut(s)	58, 225, 834
XmiI	GTMKAC	1 cut(s)	1008
XspI	CTAG	4 cut(s)	108, 813, 1227, 1619
Zsp2I	ATGCAT	1 cut(s)	310

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.