MD04G1179500.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0015399
Physical Location & Seq
Reverse (-)
27050805 .. 27051413
609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1179500.v1.1.491

Sequence Viewer

Length: 609 bp
ATGAAAGCCGTGTACGGTGGAGATGCTTTCCCCCCCAGCCGCTCCCGAAGCTGCATAAACGTCTGCCTGGCGGCGACATCTGTTGTGTTGGTCGCAACCATCGTACTCGTGATCCTCTGCCTGACGGTGTTCAAAGCCAAGGATCCCACCACGACGGTTAACTCCGTCGTCTTGAAAGACCTCGACTTGGCACTCAACATTCCACGTCTCAGCGTGGACGTGAACTTGACTCTGGGCGTGGACCTATCCGTGAATAACCCGAATAAAGTCGGGTTCAAGTACAAAAACAGCACTGCGTTTTTGAACTACAGAGGGACCAACGTGGGCGAGGCCCAAATCGGGTCGGGCGAGATATTCGCGGACAGGACCAAGTCCATGAACGTGACACTGACAATCATGGCGGATCGGCTGCTGGGGAAGTCGGAGCTCTTTTCGGACGTGGTGGCCGGAACGCTGCCGTTGAACACTTTGACCAAAGTCTCCGGGGAAGTCAGCGTTTTGGGGATTTTTAAGATTCATGTCGTTTCGACCTCCTCCTGCGACTTCAGAATCGATGTCGGTAACGTCACTGTCGGACAGCAACACTGCACGTACAAGATAAAACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

203

Amino Acids

21.6

Weight (kDa)

8.99

Isoelectric Point (pI)

19.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 82 - 176 2.7e-09 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015399)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54200
fragaria_vesca FvH4_6g09270
malus_domestica MD04G1179500.v1.1 MD12G1194600.v1.1
prunus_persica Prupe.6G299500_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0459631
rosa_laevigata RLG00000025071
rosa_multiflora Rmu_sc0000240.1_g000017 Rmu_sc0023706.1_g000007
rosa_roxburghii Rroxscaffold_6G00420110
rosa_rugosa Rorug03G0036800.1
rosa_samantha Rh3BG098100 Rh3CG100100 Rh3DG099500
rosa_wichuraiana Rw0G013810 Rw0G017610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 42
AccII CGCG 1 cut(s) 359
AciI CCGC 4 cut(s) 40, 71, 359, 401
AclWI GGATC 4 cut(s) 106, 137, 150, 411
AcoI YGGCCR 1 cut(s) 444
AcuI CTGAAG 1 cut(s) 529
AfaI GTAC 4 cut(s) 14, 105, 281, 593
AfiI CCNNNNNNNGG 2 cut(s) 187, 339
AgsI TTSAA 5 cut(s) 133, 175, 277, 304, 463
AjiI CACGTC 3 cut(s) 206, 220, 439
AjnI CCWGG 1 cut(s) 66
AluBI AGCT 2 cut(s) 51, 427
AluI AGCT 2 cut(s) 51, 427
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 2 cut(s) 212, 484
AlwI GGATC 4 cut(s) 106, 137, 150, 411
AoxI GGCC 2 cut(s) 330, 444
ApeKI GCWGC 3 cut(s) 51, 409, 454
AspS9I GGNCC 4 cut(s) 241, 315, 331, 366
AsuC2I CCSGG 1 cut(s) 484
AvaII GGWCC 3 cut(s) 241, 315, 366
BamHI GGATCC 1 cut(s) 142
BanII GRGCYC 1 cut(s) 429
BauI CACGAG 1 cut(s) 107
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 3 cut(s) 38, 396, 441
BccI CCATC 1 cut(s) 107
BceAI ACGGC 1 cut(s) 442
BciT130I CCWGG 1 cut(s) 68
BcnI CCSGG 1 cut(s) 484
BcoDI GTCTC 2 cut(s) 212, 484
BfmI CTRYAG 1 cut(s) 307
BisI GCNGC 5 cut(s) 40, 52, 72, 410, 455
BlsI GCNGC 5 cut(s) 41, 53, 73, 411, 456
Bme1390I CCNGG 2 cut(s) 68, 484
Bme18I GGWCC 3 cut(s) 241, 315, 366
BmgBI CACGTC 3 cut(s) 206, 220, 439
BmgT120I GGNCC 4 cut(s) 241, 315, 331, 366
BmiI GGNNCC 2 cut(s) 144, 316
BmrFI CCNGG 2 cut(s) 68, 484
BmsI GCATC 1 cut(s) 13
BoxI GACNNNNGTC 1 cut(s) 476
BpuMI CCSGG 1 cut(s) 484
Bsa29I ATCGAT 1 cut(s) 552
BsaAI YACGTR 1 cut(s) 591
BsaJI CCNNGG 2 cut(s) 138, 483
Bsc4I CCNNNNNNNGG 2 cut(s) 187, 339
BseBI CCWGG 1 cut(s) 68
BseCI ATCGAT 1 cut(s) 552
BseDI CCNNGG 2 cut(s) 138, 483
BseLI CCNNNNNNNGG 2 cut(s) 187, 339
BseMII CTCAG 1 cut(s) 223
BseRI GAGGAG 1 cut(s) 523
BseXI GCAGC 3 cut(s) 38, 396, 441
BseYI CCCAGC 2 cut(s) 35, 412
BsgI GTGCAG 1 cut(s) 571
Bsh1236I CGCG 1 cut(s) 359
BshFI GGCC 2 cut(s) 332, 446
BshVI ATCGAT 1 cut(s) 552
BsiHKAI GWGCWC 1 cut(s) 429
BsiSI CCGG 2 cut(s) 447, 483
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 2 cut(s) 187, 339
BsmAI GTCTC 2 cut(s) 212, 484
BsmBI CGTCTC 1 cut(s) 212
BsmFI GGGAC 1 cut(s) 328
BsnI GGCC 2 cut(s) 332, 446
Bsp1286I GDGCHC 1 cut(s) 429
Bsp143I GATC 3 cut(s) 111, 142, 403
BspACI CCGC 4 cut(s) 40, 71, 359, 401
BspANI GGCC 2 cut(s) 332, 446
BspCNI CTCAG 1 cut(s) 222
BspDI ATCGAT 1 cut(s) 552
BspFNI CGCG 1 cut(s) 359
BspLI GGNNCC 2 cut(s) 144, 316
BspPI GGATC 4 cut(s) 106, 137, 150, 411
BsrBI CCGCTC 1 cut(s) 42
BssECI CCNNGG 2 cut(s) 138, 483
BssMI GATC 3 cut(s) 111, 142, 403
BssSI CACGAG 1 cut(s) 107
BssT1I CCWWGG 1 cut(s) 138
Bst2BI CACGAG 1 cut(s) 107
Bst2UI CCWGG 1 cut(s) 68
Bst4CI ACNGT 4 cut(s) 17, 127, 157, 571
BstBAI YACGTR 1 cut(s) 591
BstDEI CTNAG 1 cut(s) 209
BstFNI CGCG 1 cut(s) 359
BstKTI GATC 3 cut(s) 114, 145, 406
BstMAI GTCTC 2 cut(s) 212, 484
BstMBI GATC 3 cut(s) 111, 142, 403
BstMWI GCNNNNNNNGC 1 cut(s) 48
BstNI CCWGG 1 cut(s) 68
BstPAI GACNNNNGTC 1 cut(s) 476
BstSCI CCNGG 2 cut(s) 66, 482
BstSFI CTRYAG 1 cut(s) 307
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 3 cut(s) 38, 396, 441
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
Bsu15I ATCGAT 1 cut(s) 552
BsuRI GGCC 2 cut(s) 332, 446
BsuTUI ATCGAT 1 cut(s) 552
BtrI CACGTC 3 cut(s) 206, 220, 439
BtsI GCAGTG 2 cut(s) 291, 583
BtsIMutI CAGTG 4 cut(s) 291, 386, 567, 583
Cfr13I GGNCC 4 cut(s) 241, 315, 331, 366
ClaI ATCGAT 1 cut(s) 552
Csp6I GTAC 4 cut(s) 13, 104, 280, 592
CviAII CATG 3 cut(s) 376, 397, 518
CviJI RGCY 8 cut(s) 8, 39, 51, 137, 332, 409, 427, 446
CviKI_1 RGCY 8 cut(s) 8, 39, 51, 137, 332, 409, 427, 446
CviQI GTAC 4 cut(s) 13, 104, 280, 592
DdeI CTNAG 1 cut(s) 209
DpnI GATC 3 cut(s) 113, 144, 405
DpnII GATC 3 cut(s) 111, 142, 403
EaeI YGGCCR 1 cut(s) 444
EciI GGCGGA 1 cut(s) 416
Ecl136II GAGCTC 1 cut(s) 427
Eco130I CCWWGG 1 cut(s) 138
Eco24I GRGCYC 1 cut(s) 429
Eco47I GGWCC 3 cut(s) 241, 315, 366
Eco53kI GAGCTC 1 cut(s) 427
Eco57I CTGAAG 1 cut(s) 529
EcoICRI GAGCTC 1 cut(s) 427
EcoRII CCWGG 1 cut(s) 66
EcoT14I CCWWGG 1 cut(s) 138
EcoT38I GRGCYC 1 cut(s) 429
ErhI CCWWGG 1 cut(s) 138
Esp3I CGTCTC 1 cut(s) 212
FaeI CATG 3 cut(s) 379, 400, 521
FaiI YATR 5 cut(s) 56, 377, 398, 519, 607
FaqI GGGAC 1 cut(s) 328
FatI CATG 3 cut(s) 375, 396, 517
Fnu4HI GCNGC 5 cut(s) 40, 52, 72, 410, 455
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 5 cut(s) 40, 52, 72, 410, 455
GluI GCNGC 5 cut(s) 40, 52, 72, 410, 455
GsaI CCCAGC 2 cut(s) 39, 416
HaeIII GGCC 2 cut(s) 332, 446
HapII CCGG 2 cut(s) 447, 483
Hin1II CATG 3 cut(s) 379, 400, 521
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 3 cut(s) 229, 514, 549
HpaI GTTAAC 1 cut(s) 160
HpaII CCGG 2 cut(s) 447, 483
Hpy166II GTNNAC 5 cut(s) 13, 160, 217, 223, 241
Hpy188I TCNGA 4 cut(s) 424, 436, 548, 575
Hpy188III TCNNGA 3 cut(s) 45, 109, 172
Hpy8I GTNNAC 5 cut(s) 13, 160, 217, 223, 241
Hpy99I CGWCG 2 cut(s) 157, 170
HpyCH4III ACNGT 4 cut(s) 17, 127, 157, 571
HpyCH4IV ACGT 8 cut(s) 60, 205, 219, 321, 381, 438, 564, 590
HpyCH4V TGCA 2 cut(s) 54, 588
HpyF10VI GCNNNNNNNGC 1 cut(s) 48
HpyF3I CTNAG 1 cut(s) 209
HpySE526I ACGT 8 cut(s) 60, 205, 219, 321, 381, 438, 564, 590
Hsp92II CATG 3 cut(s) 379, 400, 521
KspAI GTTAAC 1 cut(s) 160
Kzo9I GATC 3 cut(s) 111, 142, 403
LmnI GCTCC 2 cut(s) 47, 424
Lsp1109I GCAGC 3 cut(s) 38, 396, 441
LweI GCATC 1 cut(s) 13
MaeII ACGT 8 cut(s) 60, 205, 219, 321, 381, 438, 564, 590
MaeIII GTNAC 3 cut(s) 382, 560, 565
MalI GATC 3 cut(s) 113, 144, 405
MbiI CCGCTC 1 cut(s) 42
MboI GATC 3 cut(s) 111, 142, 403
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 1 cut(s) 429
MlyI GAGTC 1 cut(s) 223
MmeI TCCRAC 2 cut(s) 402, 553
MnlI CCTC 6 cut(s) 125, 191, 305, 322, 541, 544
MseI TTAA 2 cut(s) 159, 510
MslI CAYNNNNRTG 1 cut(s) 380
MspI CCGG 2 cut(s) 447, 483
MspR9I CCNGG 2 cut(s) 68, 484
MvaI CCWGG 1 cut(s) 68
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 1 cut(s) 48
NciI CCSGG 1 cut(s) 484
NdeII GATC 3 cut(s) 111, 142, 403
NlaIII CATG 3 cut(s) 379, 400, 521
NlaIV GGNNCC 2 cut(s) 144, 316
NmuCI GTSAC 2 cut(s) 382, 565
PcsI WCGNNNNNNNCGW 2 cut(s) 99, 345
PfeI GAWTC 2 cut(s) 514, 549
PflFI GACNNNGTC 1 cut(s) 370
PkrI GCNGC 5 cut(s) 41, 53, 73, 411, 456
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
Ppu21I YACGTR 1 cut(s) 591
PshAI GACNNNNGTC 1 cut(s) 476
Psp124BI GAGCTC 1 cut(s) 429
Psp6I CCWGG 1 cut(s) 66
PspFI CCCAGC 2 cut(s) 35, 412
PspGI CCWGG 1 cut(s) 66
PspN4I GGNNCC 2 cut(s) 144, 316
PspPI GGNCC 4 cut(s) 241, 315, 331, 366
PsuI RGATCY 1 cut(s) 142
PsyI GACNNNGTC 1 cut(s) 370
RsaI GTAC 4 cut(s) 14, 105, 281, 593
RsaNI GTAC 4 cut(s) 13, 104, 280, 592
RseI CAYNNNNRTG 1 cut(s) 380
SacI GAGCTC 1 cut(s) 429
SaqAI TTAA 2 cut(s) 159, 510
SatI GCNGC 5 cut(s) 40, 52, 72, 410, 455
Sau3AI GATC 3 cut(s) 111, 142, 403
Sau96I GGNCC 4 cut(s) 241, 315, 331, 366
SchI GAGTC 1 cut(s) 223
ScrFI CCNGG 2 cut(s) 68, 484
SduI GDGCHC 1 cut(s) 429
SfaNI GCATC 1 cut(s) 13
SfcI CTRYAG 1 cut(s) 307
SinI GGWCC 3 cut(s) 241, 315, 366
SmiMI CAYNNNNRTG 1 cut(s) 380
SsiI CCGC 4 cut(s) 40, 71, 359, 401
SstI GAGCTC 1 cut(s) 429
StyD4I CCNGG 2 cut(s) 66, 482
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 4 cut(s) 17, 127, 157, 571
TaiI ACGT 8 cut(s) 63, 208, 222, 324, 384, 441, 567, 593
TaqI TCGA 3 cut(s) 183, 527, 552
TatI WGTACW 1 cut(s) 279
TauI GCSGC 2 cut(s) 42, 74
TfiI GAWTC 2 cut(s) 514, 549
Tru1I TTAA 2 cut(s) 159, 510
Tru9I TTAA 2 cut(s) 159, 510
TscAI CASTG 4 cut(s) 298, 393, 574, 590
TseFI GTSAC 2 cut(s) 382, 565
TseI GCWGC 3 cut(s) 51, 409, 454
Tsp45I GTSAC 2 cut(s) 382, 565
TspDTI ATGAA 3 cut(s) 17, 392, 506
TspGWI ACGGA 2 cut(s) 154, 238
TspRI CASTG 4 cut(s) 298, 393, 574, 590
Tth111I GACNNNGTC 1 cut(s) 370
VpaK11BI GGWCC 3 cut(s) 241, 315, 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.