Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0015647

Physical Location & Seq

Genomic Coordinates Reverse (-)

27600080 .. 27602484

Gene Structure 2405 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1184900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1245 bp

ATGGGGAGGCACTCTTGTTGCTACAAGCAGAAGCTAAGGAAAGGCCTCTGGTCTCCTGAAGAGGATGAGAAGCTTCTCAATTACATCACCAAGCATGGCCATGGCTGCTGGAGCTCAGTCCCTAAACTAGCAGGTCTGCAGAGGTGTGGGAAGAGCTGCAGGTTAAGGTGGATAAATTACCTGAGGCCCGATTTGAAGAGAGGGCCATTCTCACAGCAAGAAGAGAATTTGATAATAGAACTCCATGCAGTTCTTGGCAACAGATGGTCTCAGATTGCAGCCCAGTTACCAGGAAGAACAGACAATGAGATTAAAAATCTATGGAATTCCTGCATTAAGAAGAAGCTGAGGCTAAGAGGCATTGACCCCAACACTCACAAACCACTCTCAGAAGTGCTTGATCAAAATATTGGCAAAGAAATTAACAACAACAATAATATTAAGCTCTCCCCAACTTACAAAAGCAATGAGAAGGCCTCTGTGGGATCCAATGAGCTGAGCCTGGTTGAGGCAGTGAGTTCAAGGCACCCTACTTCAGCGTCCGAAAACCGGTTCAACCCGGTCGAAGTTTCTTCCACCTCCAAACTCATAAGCAGCAATGGCGGCAGCAGCAAAAGTTTGACACATGAAGGCTCCCCCAGTAGTTGCAGGCCTTGTGATTTTGTGGGATACTTTTCTTTTCCTCATCACAATAACCAAAGCAATTATGGTTCGTCGTCGGATATGGGGCTGCAGGCAGTAAATCAAAACACCACTTTCAGCTTCCTCAATCAAAATCCAGAGTTCCTGCAGCCGTCCATGTCATCAAGCTCCGCCATATTCACCGCCCCTCCTCCTCCTCCCACACGTGTGAAGCCTTCCATCAGCCTCCCATCCGATAACTCCTCCACCTGGGACTCCAATGCCAACAACAGCAACAGCAGCGGCGGCGGCTACTTCGACAGCTCTGCAGCCTTTTCTTGGTGGCCGCCGGAGGCGGTAAAATCGGACGACCCAGAAGAGATCAAATGGTCTGAATATCTCCACAACACCTCATTTCTGATGGGAACCCACCAAGCCTCTCAGCCTGCTACTTACTCAGAAATCAAACCAGAATCTCAGTCACACATTTTATCAAACAGCTTGAGTGCAACATCTTGGCACCACCAGAACCAGCAGCAGCAGCAGCAGCAAGCTTTACAAGCTTCAGAGATGTACACAAAGGATCTTCAGAGACTTGCTGTATCTTTTGGTCAAACCCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

415

Amino Acids

46.09

Weight (kDa)

8.52

Isoelectric Point (pI)

65.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	14 - 61	2.9e-17	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	17 - 75	4.4e-13	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	67 - 110	3.9e-15	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	70 - 115	1.8e-06	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015647)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g08620
malus_domestica	MD04G1184900.v1.1 MD12G1198600.v1.1
prunus_persica	Prupe.6G303300_v2.0.a1
pyrus_communis	pycom04g16420 pycom12g18500
rosa_chinensis	RchiOBHm_Chr3g0458721
rosa_laevigata	RLG00000025139
rosa_multiflora	Rmu_sc0001634.1_g000020
rosa_roxburghii	Rroxscaffold_6G00420840
rosa_rugosa	Rorug03G0031500
rosa_samantha	Rh3AG089100 Rh3BG091900 Rh3CG092300 Rh3DG093000
rosa_wichuraiana	Rw3G007620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	122, 150
AccB1I	GGYRCC	2 cut(s)	525, 1140
AciI	CCGC	8 cut(s)	603, 813, 825, 924, 927, 930, 968, 977
AclWI	GGATC	3 cut(s)	480, 493, 1212
AcoI	YGGCCR	2 cut(s)	97, 965
AcsI	RAATTY	2 cut(s)	226, 325
AcuI	CTGAAG	4 cut(s)	78, 519, 1170, 1193
AcvI	CACGTG	1 cut(s)	848
AfaI	GTAC	1 cut(s)	1196
AfiI	CCNNNNNNNGG	4 cut(s)	508, 549, 891, 960
AflIII	ACRYGT	2 cut(s)	845, 847
AgeI	ACCGGT	1 cut(s)	549
AgsI	TTSAA	3 cut(s)	196, 522, 556
AjnI	CCWGG	3 cut(s)	289, 501, 890
AleI	CACNNNNGTG	1 cut(s)	848
Alw21I	GWGCWC	1 cut(s)	116
Alw26I	GTCTC	3 cut(s)	57, 273, 1207
AlwI	GGATC	3 cut(s)	480, 493, 1212
AoxI	GGCC	7 cut(s)	43, 97, 185, 203, 474, 650, 965
ApoI	RAATTY	2 cut(s)	226, 325
AsiGI	ACCGGT	1 cut(s)	549
AspS9I	GGNCC	2 cut(s)	186, 203
AsuC2I	CCSGG	1 cut(s)	560
AsuHPI	GGTGA	2 cut(s)	79, 814
AxyI	CCTNAGG	1 cut(s)	182
BalI	TGGCCA	1 cut(s)	99
BamHI	GGATCC	1 cut(s)	485
BanI	GGYRCC	2 cut(s)	525, 1140
BanII	GRGCYC	1 cut(s)	116
BbrPI	CACGTG	1 cut(s)	848
Bbv12I	GWGCWC	1 cut(s)	116
BbvCI	CCTCAGC	1 cut(s)	347
BccI	CCATC	4 cut(s)	258, 869, 880, 1036
BceAI	ACGGC	1 cut(s)	778
BcgI	CGANNNNNNTGC	2 cut(s)	929, 963
BciT130I	CCWGG	3 cut(s)	291, 503, 892
BciVI	GTATCC	1 cut(s)	662
BclI	TGATCA	1 cut(s)	400
BcnI	CCSGG	1 cut(s)	560
BcoDI	GTCTC	3 cut(s)	57, 273, 1207
BfaI	CTAG	1 cut(s)	128
BfmI	CTRYAG	5 cut(s)	137, 157, 731, 788, 948
BfuAI	ACCTGC	2 cut(s)	122, 150
BfuI	GTATCC	1 cut(s)	662
BlpI	GCTNAGC	1 cut(s)	497
Bme1390I	CCNGG	4 cut(s)	291, 503, 560, 892
BmgT120I	GGNCC	2 cut(s)	186, 203
BmiI	GGNNCC	5 cut(s)	487, 527, 634, 1048, 1142
BmrFI	CCNGG	4 cut(s)	291, 503, 560, 892
BmrI	ACTGGG	2 cut(s)	277, 633
BmuI	ACTGGG	2 cut(s)	277, 633
BplI	GAGNNNNNCTC	2 cut(s)	461, 493
BpmI	CTGGAG	1 cut(s)	130
Bpu10I	CCTNAGC	2 cut(s)	35, 347
Bpu1102I	GCTNAGC	1 cut(s)	497
BpuEI	CTTGAG	1 cut(s)	1144
BpuMI	CCSGG	1 cut(s)	560
BsaAI	YACGTR	1 cut(s)	848
BsaI	GGTCTC	2 cut(s)	57, 273
BsaJI	CCNNGG	2 cut(s)	100, 891
BsaWI	WCCGGW	1 cut(s)	549
BsaXI	ACNNNNNCTCC	2 cut(s)	814, 844
Bsc4I	CCNNNNNNNGG	4 cut(s)	508, 549, 891, 960
Bse118I	RCCGGY	1 cut(s)	549
Bse1I	ACTGG	2 cut(s)	283, 639
Bse21I	CCTNAGG	1 cut(s)	182
Bse3DI	GCAATG	2 cut(s)	472, 604
BseBI	CCWGG	3 cut(s)	291, 503, 892
BseDI	CCNNGG	2 cut(s)	100, 891
BseGI	GGATG	2 cut(s)	70, 872
BseLI	CCNNNNNNNGG	4 cut(s)	508, 549, 891, 960
BseMI	GCAATG	2 cut(s)	472, 604
BseMII	CTCAG	9 cut(s)	129, 173, 284, 338, 402, 488, 1076, 1092, 1112
BseNI	ACTGG	2 cut(s)	283, 639
BseRI	GAGGAG	4 cut(s)	822, 825, 828, 874
Bsh1285I	CGRYCG	1 cut(s)	564
BshFI	GGCC	7 cut(s)	45, 99, 187, 205, 476, 652, 967
BshNI	GGYRCC	2 cut(s)	525, 1140
BshTI	ACCGGT	1 cut(s)	549
BsiEI	CGRYCG	1 cut(s)	564
BsiHKAI	GWGCWC	1 cut(s)	116
BsiSI	CCGG	3 cut(s)	550, 560, 971
BslFI	GGGAC	2 cut(s)	104, 908
BslI	CCNNNNNNNGG	4 cut(s)	508, 549, 891, 960
BsmAI	GTCTC	3 cut(s)	57, 273, 1207
BsmFI	GGGAC	2 cut(s)	104, 908
BsnI	GGCC	7 cut(s)	45, 99, 187, 205, 476, 652, 967
Bso31I	GGTCTC	2 cut(s)	57, 273
Bsp1286I	GDGCHC	1 cut(s)	116
Bsp1407I	TGTACA	1 cut(s)	1194
Bsp143I	GATC	4 cut(s)	400, 485, 1002, 1204
Bsp1720I	GCTNAGC	1 cut(s)	497
Bsp19I	CCATGG	1 cut(s)	100
BspACI	CCGC	8 cut(s)	603, 813, 825, 924, 927, 930, 968, 977
BspANI	GGCC	7 cut(s)	45, 99, 187, 205, 476, 652, 967
BspCNI	CTCAG	9 cut(s)	128, 174, 283, 339, 401, 489, 1075, 1091, 1111
BspLI	GGNNCC	5 cut(s)	487, 527, 634, 1048, 1142
BspMAI	CTGCAG	5 cut(s)	141, 161, 735, 792, 952
BspMI	ACCTGC	2 cut(s)	122, 150
BspPI	GGATC	3 cut(s)	480, 493, 1212
BspQI	GCTCTTC	1 cut(s)	146
BspT107I	GGYRCC	2 cut(s)	525, 1140
BspTNI	GGTCTC	2 cut(s)	57, 273
BsrDI	GCAATG	2 cut(s)	472, 604
BsrFI	RCCGGY	1 cut(s)	549
BsrGI	TGTACA	1 cut(s)	1194
BsrI	ACTGG	2 cut(s)	283, 639
BssAI	RCCGGY	1 cut(s)	549
BssECI	CCNNGG	2 cut(s)	100, 891
BssMI	GATC	4 cut(s)	400, 485, 1002, 1204
BssT1I	CCWWGG	1 cut(s)	100
Bst2UI	CCWGG	3 cut(s)	291, 503, 892
Bst6I	CTCTTC	5 cut(s)	54, 146, 191, 216, 993
BstAUI	TGTACA	1 cut(s)	1194
BstBAI	YACGTR	1 cut(s)	848
BstC8I	GCNNGC	4 cut(s)	650, 735, 1068, 1173
BstDSI	CCRYGG	1 cut(s)	100
BstENI	CCTNNNNNAGG	1 cut(s)	506
BstF5I	GGATG	2 cut(s)	70, 872
BstKTI	GATC	4 cut(s)	403, 488, 1005, 1207
BstMAI	GTCTC	3 cut(s)	57, 273, 1207
BstMBI	GATC	4 cut(s)	400, 485, 1002, 1204
BstMCI	CGRYCG	1 cut(s)	564
BstNI	CCWGG	3 cut(s)	291, 503, 892
BstSCI	CCNGG	4 cut(s)	289, 501, 558, 890
BstSFI	CTRYAG	5 cut(s)	137, 157, 731, 788, 948
BstX2I	RGATCY	2 cut(s)	485, 1204
BstYI	RGATCY	2 cut(s)	485, 1204
Bsu36I	CCTNAGG	1 cut(s)	182
BsuI	GTATCC	1 cut(s)	662
BsuRI	GGCC	7 cut(s)	45, 99, 187, 205, 476, 652, 967
BtgI	CCRYGG	1 cut(s)	100
BtsCI	GGATG	2 cut(s)	70, 872
BtsI	GCAGTG	1 cut(s)	519
BtsIMutI	CAGTG	1 cut(s)	519
BveI	ACCTGC	2 cut(s)	122, 150
Cac8I	GCNNGC	4 cut(s)	650, 735, 1068, 1173
Cfr10I	RCCGGY	1 cut(s)	549
Cfr13I	GGNCC	2 cut(s)	186, 203
CseI	GACGC	1 cut(s)	528
Csp6I	GTAC	1 cut(s)	1195
CspAI	ACCGGT	1 cut(s)	549
CviAII	CATG	5 cut(s)	95, 101, 245, 626, 799
CviQI	GTAC	1 cut(s)	1195
DpnI	GATC	4 cut(s)	402, 487, 1004, 1206
DpnII	GATC	4 cut(s)	400, 485, 1002, 1204
EaeI	YGGCCR	2 cut(s)	97, 965
Eam1104I	CTCTTC	5 cut(s)	54, 146, 191, 216, 993
EarI	CTCTTC	5 cut(s)	54, 146, 191, 216, 993
EciI	GGCGGA	1 cut(s)	802
Ecl136II	GAGCTC	1 cut(s)	114
Eco130I	CCWWGG	1 cut(s)	100
Eco147I	AGGCCT	3 cut(s)	45, 476, 652
Eco24I	GRGCYC	1 cut(s)	116
Eco31I	GGTCTC	2 cut(s)	57, 273
Eco53kI	GAGCTC	1 cut(s)	114
Eco57I	CTGAAG	4 cut(s)	78, 519, 1170, 1193
Eco72I	CACGTG	1 cut(s)	848
Eco81I	CCTNAGG	1 cut(s)	182
EcoICRI	GAGCTC	1 cut(s)	114
EcoNI	CCTNNNNNAGG	1 cut(s)	506
EcoRI	GAATTC	1 cut(s)	325
EcoRII	CCWGG	3 cut(s)	289, 501, 890
EcoT14I	CCWWGG	1 cut(s)	100
EcoT38I	GRGCYC	1 cut(s)	116
ErhI	CCWWGG	1 cut(s)	100
FaeI	CATG	5 cut(s)	98, 104, 248, 629, 802
FaqI	GGGAC	2 cut(s)	104, 908
FatI	CATG	5 cut(s)	94, 100, 244, 625, 798
FbaI	TGATCA	1 cut(s)	400
FokI	GGATG	2 cut(s)	77, 859
FriOI	GRGCYC	1 cut(s)	116
FspBI	CTAG	1 cut(s)	128
GsuI	CTGGAG	1 cut(s)	130
HaeIII	GGCC	7 cut(s)	45, 99, 187, 205, 476, 652, 967
HapII	CCGG	3 cut(s)	550, 560, 971
HgaI	GACGC	1 cut(s)	528
Hin1II	CATG	5 cut(s)	98, 104, 248, 629, 802
HindIII	AAGCTT	3 cut(s)	71, 1173, 1182
HinfI	GANTC	2 cut(s)	896, 1094
HpaII	CCGG	3 cut(s)	550, 560, 971
HphI	GGTGA	2 cut(s)	79, 814
Hpy166II	GTNNAC	1 cut(s)	1197
Hpy188III	TCNNGA	2 cut(s)	56, 779
Hpy8I	GTNNAC	1 cut(s)	1197
Hpy99I	CGWCG	2 cut(s)	718, 721
HpyAV	CCTTC	3 cut(s)	466, 623, 867
HpyCH4IV	ACGT	1 cut(s)	847
HpySE526I	ACGT	1 cut(s)	847
Hsp92II	CATG	5 cut(s)	98, 104, 248, 629, 802
Ksp22I	TGATCA	1 cut(s)	400
Kzo9I	GATC	4 cut(s)	400, 485, 1002, 1204
LguI	GCTCTTC	1 cut(s)	146
LmnI	GCTCC	3 cut(s)	111, 638, 815
MaeI	CTAG	1 cut(s)	128
MaeII	ACGT	1 cut(s)	847
MaeIII	GTNAC	2 cut(s)	285, 1101
MalI	GATC	4 cut(s)	402, 487, 1004, 1206
MboI	GATC	4 cut(s)	400, 485, 1002, 1204
MboII	GAAGA	9 cut(s)	71, 163, 208, 233, 306, 352, 564, 1010, 1199
MflI	RGATCY	2 cut(s)	485, 1204
MhlI	GDGCHC	1 cut(s)	116
MlsI	TGGCCA	1 cut(s)	99
MluCI	AATT	6 cut(s)	79, 175, 226, 325, 420, 703
MluNI	TGGCCA	1 cut(s)	99
MlyI	GAGTC	1 cut(s)	890
MmeI	TCCRAC	1 cut(s)	699
Mox20I	TGGCCA	1 cut(s)	99
MscI	TGGCCA	1 cut(s)	99
MseI	TTAA	5 cut(s)	164, 312, 336, 423, 441
MslI	CAYNNNNRTG	2 cut(s)	99, 848
Msp20I	TGGCCA	1 cut(s)	99
MspA1I	CMGCKG	1 cut(s)	924
MspI	CCGG	3 cut(s)	550, 560, 971
MspR9I	CCNGG	4 cut(s)	291, 503, 560, 892
MvaI	CCWGG	3 cut(s)	291, 503, 892
NciI	CCSGG	1 cut(s)	560
NcoI	CCATGG	1 cut(s)	100
NdeII	GATC	4 cut(s)	400, 485, 1002, 1204
NlaIII	CATG	5 cut(s)	98, 104, 248, 629, 802
NlaIV	GGNNCC	5 cut(s)	487, 527, 634, 1048, 1142
NmuCI	GTSAC	1 cut(s)	1101
OliI	CACNNNNGTG	1 cut(s)	848
PceI	AGGCCT	3 cut(s)	45, 476, 652
PciSI	GCTCTTC	1 cut(s)	146
PfeI	GAWTC	1 cut(s)	1094
PinAI	ACCGGT	1 cut(s)	549
PleI	GAGTC	1 cut(s)	890
PmaCI	CACGTG	1 cut(s)	848
PmlI	CACGTG	1 cut(s)	848
PpsI	GAGTC	1 cut(s)	890
Ppu21I	YACGTR	1 cut(s)	848
Psp124BI	GAGCTC	1 cut(s)	116
Psp6I	CCWGG	3 cut(s)	289, 501, 890
PspCI	CACGTG	1 cut(s)	848
PspGI	CCWGG	3 cut(s)	289, 501, 890
PspN4I	GGNNCC	5 cut(s)	487, 527, 634, 1048, 1142
PspPI	GGNCC	2 cut(s)	186, 203
PstI	CTGCAG	5 cut(s)	141, 161, 735, 792, 952
PsuI	RGATCY	2 cut(s)	485, 1204
RsaI	GTAC	1 cut(s)	1196
RsaNI	GTAC	1 cut(s)	1195
RseI	CAYNNNNRTG	2 cut(s)	99, 848
SacI	GAGCTC	1 cut(s)	116
SapI	GCTCTTC	1 cut(s)	146
SaqAI	TTAA	5 cut(s)	164, 312, 336, 423, 441
Sau3AI	GATC	4 cut(s)	400, 485, 1002, 1204
Sau96I	GGNCC	2 cut(s)	186, 203
SchI	GAGTC	1 cut(s)	890
ScrFI	CCNGG	4 cut(s)	291, 503, 560, 892
SduI	GDGCHC	1 cut(s)	116
SfcI	CTRYAG	5 cut(s)	137, 157, 731, 788, 948
SmiMI	CAYNNNNRTG	2 cut(s)	99, 848
SmlI	CTYRAG	1 cut(s)	1123
SmoI	CTYRAG	1 cut(s)	1123
Sse9I	AATT	6 cut(s)	79, 175, 226, 325, 420, 703
SseBI	AGGCCT	3 cut(s)	45, 476, 652
SsiI	CCGC	8 cut(s)	603, 813, 825, 924, 927, 930, 968, 977
SspI	AATATT	2 cut(s)	409, 439
SspMI	CTAG	1 cut(s)	128
SstI	GAGCTC	1 cut(s)	116
StuI	AGGCCT	3 cut(s)	45, 476, 652
StyD4I	CCNGG	4 cut(s)	289, 501, 558, 890
StyI	CCWWGG	1 cut(s)	100
TaiI	ACGT	1 cut(s)	850
TaqI	TCGA	2 cut(s)	564, 939
TasI	AATT	6 cut(s)	79, 175, 226, 325, 420, 703
TatI	WGTACW	1 cut(s)	1194
TauI	GCSGC	5 cut(s)	606, 927, 930, 933, 970
TfiI	GAWTC	1 cut(s)	1094
Tru1I	TTAA	5 cut(s)	164, 312, 336, 423, 441
Tru9I	TTAA	5 cut(s)	164, 312, 336, 423, 441
TscAI	CASTG	1 cut(s)	519
TseFI	GTSAC	1 cut(s)	1101
Tsp45I	GTSAC	1 cut(s)	1101
TspDTI	ATGAA	1 cut(s)	642
TspRI	CASTG	1 cut(s)	519
XagI	CCTNNNNNAGG	1 cut(s)	506
XapI	RAATTY	2 cut(s)	226, 325
XcmI	CCANNNNNNNNNTGG	2 cut(s)	251, 704
XspI	CTAG	1 cut(s)	128

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.