Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0009520

Physical Location & Seq

Genomic Coordinates Reverse (-)

27791017 .. 27795882

Gene Structure 4866 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1187300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1866 bp

ATGGACTTCACTACAGGTGATGCAGTCGCCGATCGAGAGCAACAAGGTGAAGCGATTTATCGGAGAAGAAAGCCCCAAAAACCACTTCCAGATTTAGAACCCTCGAAGCAGGCGTGCTCGACTCGCTATGTGGGAGGAAGGGCGAGTGTGGCATCTGATGCTGCCGTCGGAGCTCTGGTCAGGAAAACCAGTTGGTCTCGATCCCTTTCGACAAGAGGGAGGACAAGTATTGCTGTTGGTGCTTTCTTTGAATATCATCCTCCACAGAAGGAGCCCAAAAGAAGAGGAAAAAGAAAGGGAAAAGCTCCTCTTCCCAAAGGAAAGTACAAATTCGAAGAGGAGAAGGTGTACTTTCAAGAGGTTGATGCCTTTGATTTGTTGGTGGAGAGCCCCTCTCCCAAACCTACTACATGGGTTGCCGGCAACCCAGCAGATGATGCTGCATTACCCAAATTGTGCTCACCATTAGATAAATGGTTATTCAAGAAGTTCAATTTCACATTTGTCCCTTCTAGAACATTGTCTAAGATACTACAAACTTCAGCCATTCGGCCTATGCCTTTGGAAACTGTTGGTGGTAGGAATTTCACATCTTCAAGTTTGAAAACCCCACAAAACTATACTAAATTCAGTTCTCGCTTGCCCTGTACCCAAAGCATATTGGACTTCAGTTTGGCTGAATTTAGTTTGCGTGAGAGTGGAAGCATAGAATCAATGGCCGATGCAGGCAATGATGGCTGTGAAGATATAGAAGCTTCTGTAAAGAAACTCTCTCTAGCATCATCTTCATCCGATAGTGAACAGTTAGATCCCTTTGGTGCCCTATTAAGATTATGTGGCCAGTCGGCTCCCTTAAAATTTGGGGACGTGTTCTCTACATACTGTGACCTAGAAAAGATTAACAAAATTGGTGAAGGAACATTTGGAGAAGCTTTCATGGCTGGTAGCTATGTTTGCAAAATAGTCCCAATTGATGGCAACTTACTAGTGAACGGGGAAGTGCAAAAGACATCTAAAGAAATGCTAGAAGAGGTTATCCTTTCCCGTACACTTAACTGCTTAAAAGGACATGATAGTAATGCTCCAAACGCTTGCACCACATTTATCGGAACAATAGATTTGAGAGTGTGCCAAGGTTCTTACGATGCTGCCTTGATCAAAGCATGGGAGGATTGGGATGAAAAGTGTGGTTCAGAAAACGATCACCCCAAGGCATTTCCAGATAACCAGTGCTATGTTGTGTTTGTTTTAGATAATGGGGGCGAAGATCTTGAAAGCTTTGTGCTTATGAACATTGATGAAGCACGGAGTTTATTGGTCCAGGTTACAGCTTCCCTTGCCGTGGCTGAAGCTGCATATGAGTTTGAACACCGTGATCTTCACTGGGGAAACATCCTTTTAAGTCGGAATGATTCTGTTACAATGCCGTTGACTCTTGAGAGAAAACAGATGTTTATTCAAACATTTGGATTGGTGGTATCGATAATAGATTTCAGTCTTTCAAGAATCAGTAATGGTGAAGACGTACTCTTTCTAGACCTATCTTCAGATCCTGAACTCTTTAAAGGTCCAAAGGGAGATAGACAATCAGAAACATACCGGAAGATGAAGGAGGTAACAAATGACTGCTGGGAGGGAAGCTTCCCCAGAACAAATGTACTGTGGTTGATATACCTGGTGGATATATTACTGCTAAAGAAATCATTTGAACGTTCATCAAAGGACGAGAGAGACCTGCGCTCGTTGAAGAAGCGTCTTGACCAGTGTCAGTCAGCCAGAGAAGCACTTCTTGATCCCTCTTTCAGTGATTTGTTTGTTGATCCTCCTTGCATTGAAGTTACAATTAGGAATGACAAGATGCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000278 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006325 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016301 GO:0016310 GO:0016569 GO:0016570 GO:0016572 GO:0016740 GO:0016772 GO:0016773 GO:0018107 GO:0018193 GO:0018210 GO:0019538 GO:0023052 GO:0035173 GO:0035184 GO:0035402 GO:0035405 GO:0035407 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051276 GO:0051716 GO:0065007 GO:0071704 GO:0071840 GO:0072354 GO:0072355 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Haspin_kinase

Protein Analysis

622

Amino Acids

69.25

Weight (kDa)

5.48

Isoelectric Point (pI)

45.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Haspin_kinase	PF12330	274 - 593	7e-50	Haspin like kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009520)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09450
fragaria_vesca	FvH4_6g08390 FvH4_6g08390 FvH4_6g08390 FvH4_6g08390 FvH4_6g08390 FvH4_6g08390 FvH4_6g08390 FvH4_6g08390
malus_domestica	MD04G1187300.v1.1
prunus_persica	Prupe.6G305800_v2.0.a1
pyrus_communis	pycom04g16540
rosa_chinensis	RchiOBHm_Chr3g0458361
rosa_laevigata	RLG00000025166
rosa_multiflora	Rmu_sc0007338.1_g000011
rosa_roxburghii	Rroxscaffold_6G00421150
rosa_rugosa	Rorug03G0026000
rosa_samantha	Rh3AG086900 Rh3BG089700 Rh3CG090100 Rh3DG090700
rosa_wichuraiana	Rw3G007380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1743
AccB1I	GGYRCC	1 cut(s)	818
AccB7I	CCANNNNNTGG	1 cut(s)	974
AclI	AACGTT	1 cut(s)	1711
AclWI	GGATC	5 cut(s)	195, 803, 1544, 1787, 1814
AcoI	YGGCCR	2 cut(s)	717, 838
AcsI	RAATTY	5 cut(s)	329, 583, 626, 680, 857
AcuI	CTGAAG	4 cut(s)	525, 652, 1368, 1530
AfaI	GTAC	6 cut(s)	326, 350, 649, 1048, 1527, 1659
AfiI	CCNNNNNNNGG	2 cut(s)	974, 1342
AflIII	ACRYGT	1 cut(s)	867
AhlI	ACTAGT	1 cut(s)	985
AjiI	CACGTC	1 cut(s)	868
AjnI	CCWGG	2 cut(s)	1320, 1674
AjuI	GAANNNNNNNTTGG	4 cut(s)	69, 101, 906, 938
AluBI	AGCT	9 cut(s)	173, 305, 755, 932, 948, 1278, 1331, 1352, 1641
AluI	AGCT	9 cut(s)	173, 305, 755, 932, 948, 1278, 1331, 1352, 1641
Alw21I	GWGCWC	3 cut(s)	119, 175, 461
Alw26I	GTCTC	2 cut(s)	201, 1725
AlwI	GGATC	5 cut(s)	195, 803, 1544, 1787, 1814
AlwNI	CAGNNNCTG	1 cut(s)	1553
AoxI	GGCC	3 cut(s)	551, 717, 838
ApeKI	GCWGC	4 cut(s)	161, 440, 1148, 1352
ApoI	RAATTY	5 cut(s)	329, 583, 626, 680, 857
Asp700I	GAANNNNTTC	1 cut(s)	1785
AspLEI	GCGC	1 cut(s)	1740
AspS9I	GGNCC	2 cut(s)	1318, 1568
AsuHPI	GGTGA	6 cut(s)	29, 59, 453, 923, 1196, 1529
AsuII	TTCGAA	1 cut(s)	333
AvaII	GGWCC	2 cut(s)	1318, 1568
BaeGI	GKGCMC	1 cut(s)	823
BalI	TGGCCA	1 cut(s)	840
BanI	GGYRCC	1 cut(s)	818
BanII	GRGCYC	3 cut(s)	175, 276, 392
BbsI	GAAGAC	1 cut(s)	1527
Bbv12I	GWGCWC	3 cut(s)	119, 175, 461
BbvI	GCAGC	4 cut(s)	148, 427, 1135, 1339
BccI	CCATC	2 cut(s)	728, 968
BceAI	ACGGC	3 cut(s)	149, 1325, 1411
BciT130I	CCWGG	2 cut(s)	1322, 1676
BclI	TGATCA	1 cut(s)	1155
BcoDI	GTCTC	2 cut(s)	201, 1725
BcuI	ACTAGT	1 cut(s)	985
BfaI	CTAG	6 cut(s)	513, 776, 890, 986, 1025, 1535
BfmI	CTRYAG	2 cut(s)	12, 1862
BfuAI	ACCTGC	1 cut(s)	1743
BglII	AGATCT	1 cut(s)	1267
BisI	GCNGC	4 cut(s)	162, 441, 1149, 1353
BlsI	GCNGC	4 cut(s)	163, 442, 1150, 1354
Bme1390I	CCNGG	2 cut(s)	1322, 1676
Bme18I	GGWCC	2 cut(s)	1318, 1568
BmgBI	CACGTC	1 cut(s)	868
BmgT120I	GGNCC	2 cut(s)	1318, 1568
BmiI	GGNNCC	3 cut(s)	273, 820, 849
BmrFI	CCNGG	2 cut(s)	1322, 1676
BmrI	ACTGGG	1 cut(s)	1393
BmsI	GCATC	9 cut(s)	10, 148, 161, 355, 427, 712, 788, 1135, 1848
BmuI	ACTGGG	1 cut(s)	1393
BoxI	GACNNNNGTC	1 cut(s)	1764
BpiI	GAAGAC	1 cut(s)	1527
BplI	GAGNNNNNCTC	4 cut(s)	377, 409, 379, 411
Bpu14I	TTCGAA	1 cut(s)	333
BpuEI	CTTGAG	1 cut(s)	1457
Bsa29I	ATCGAT	1 cut(s)	1481
BsaI	GGTCTC	2 cut(s)	201, 1725
BsaJI	CCNNGG	3 cut(s)	1132, 1209, 1341
BsaWI	WCCGGW	1 cut(s)	1599
BsaXI	ACNNNNNCTCC	6 cut(s)	162, 192, 211, 241, 332, 362
Bsc4I	CCNNNNNNNGG	2 cut(s)	974, 1342
Bse118I	RCCGGY	1 cut(s)	419
Bse1I	ACTGG	5 cut(s)	189, 841, 1228, 1388, 1762
Bse3DI	GCAATG	1 cut(s)	736
BseBI	CCWGG	2 cut(s)	1322, 1676
BseCI	ATCGAT	1 cut(s)	1481
BseDI	CCNNGG	3 cut(s)	1132, 1209, 1341
BseGI	GGATG	4 cut(s)	256, 788, 1183, 1392
BseLI	CCNNNNNNNGG	2 cut(s)	974, 1342
BseMI	GCAATG	1 cut(s)	736
BseNI	ACTGG	5 cut(s)	189, 841, 1228, 1388, 1762
BseRI	GAGGAG	2 cut(s)	297, 353
BseSI	GKGCMC	1 cut(s)	823
BseXI	GCAGC	4 cut(s)	148, 427, 1135, 1339
BseYI	CCCAGC	2 cut(s)	427, 1629
Bsh1285I	CGRYCG	1 cut(s)	34
BshFI	GGCC	3 cut(s)	553, 719, 840
BshNI	GGYRCC	1 cut(s)	818
BshVI	ATCGAT	1 cut(s)	1481
BsiEI	CGRYCG	1 cut(s)	34
BsiHKAI	GWGCWC	3 cut(s)	119, 175, 461
BsiSI	CCGG	2 cut(s)	420, 1600
BslFI	GGGAC	3 cut(s)	491, 878, 950
BslI	CCNNNNNNNGG	2 cut(s)	974, 1342
BsmAI	GTCTC	2 cut(s)	201, 1725
BsmFI	GGGAC	3 cut(s)	491, 878, 950
BsnI	GGCC	3 cut(s)	553, 719, 840
Bso31I	GGTCTC	2 cut(s)	201, 1725
Bsp119I	TTCGAA	1 cut(s)	333
Bsp1286I	GDGCHC	6 cut(s)	119, 175, 276, 392, 461, 823
BspANI	GGCC	3 cut(s)	553, 719, 840
BspDI	ATCGAT	1 cut(s)	1481
BspLI	GGNNCC	3 cut(s)	273, 820, 849
BspMI	ACCTGC	1 cut(s)	1743
BspPI	GGATC	5 cut(s)	195, 803, 1544, 1787, 1814
BspT104I	TTCGAA	1 cut(s)	333
BspT107I	GGYRCC	1 cut(s)	818
BspTNI	GGTCTC	2 cut(s)	201, 1725
BsrDI	GCAATG	1 cut(s)	736
BsrFI	RCCGGY	1 cut(s)	419
BsrI	ACTGG	5 cut(s)	189, 841, 1228, 1388, 1762
BssAI	RCCGGY	1 cut(s)	419
BssECI	CCNNGG	3 cut(s)	1132, 1209, 1341
BssT1I	CCWWGG	2 cut(s)	1132, 1209
Bst2UI	CCWGG	2 cut(s)	1322, 1676
Bst4CI	ACNGT	5 cut(s)	571, 804, 884, 1373, 1662
Bst6I	CTCTTC	4 cut(s)	277, 315, 330, 1023
BstAPI	GCANNNNNTGC	2 cut(s)	158, 437
BstBI	TTCGAA	1 cut(s)	333
BstC8I	GCNNGC	6 cut(s)	111, 115, 421, 641, 727, 1093
BstDEI	CTNAG	1 cut(s)	525
BstDSI	CCRYGG	1 cut(s)	1341
BstF5I	GGATG	4 cut(s)	256, 788, 1183, 1392
BstHHI	GCGC	1 cut(s)	1740
BstMAI	GTCTC	2 cut(s)	201, 1725
BstMCI	CGRYCG	1 cut(s)	34
BstNI	CCWGG	2 cut(s)	1322, 1676
BstPAI	GACNNNNGTC	1 cut(s)	1764
BstSCI	CCNGG	2 cut(s)	1320, 1674
BstSFI	CTRYAG	2 cut(s)	12, 1862
BstSLI	GKGCMC	1 cut(s)	823
BstV1I	GCAGC	4 cut(s)	148, 427, 1135, 1339
BstV2I	GAAGAC	1 cut(s)	1527
BstX2I	RGATCY	3 cut(s)	808, 1267, 1549
BstYI	RGATCY	3 cut(s)	808, 1267, 1549
Bsu15I	ATCGAT	1 cut(s)	1481
BsuRI	GGCC	3 cut(s)	553, 719, 840
BsuTUI	ATCGAT	1 cut(s)	1481
BtgI	CCRYGG	1 cut(s)	1341
BtrI	CACGTC	1 cut(s)	868
BtsCI	GGATG	4 cut(s)	256, 788, 1183, 1392
BtsIMutI	CAGTG	4 cut(s)	1235, 1381, 1769, 1810
BveI	ACCTGC	1 cut(s)	1743
Cac8I	GCNNGC	6 cut(s)	111, 115, 421, 641, 727, 1093
CaiI	CAGNNNCTG	1 cut(s)	1553
CfoI	GCGC	1 cut(s)	1740
Cfr10I	RCCGGY	1 cut(s)	419
Cfr13I	GGNCC	2 cut(s)	1318, 1568
ClaI	ATCGAT	1 cut(s)	1481
CseI	GACGC	1 cut(s)	1742
CsiI	ACCWGGT	1 cut(s)	1674
Csp6I	GTAC	6 cut(s)	325, 349, 648, 1047, 1526, 1658
CviAII	CATG	4 cut(s)	411, 937, 1070, 1164
CviQI	GTAC	6 cut(s)	325, 349, 648, 1047, 1526, 1658
DdeI	CTNAG	1 cut(s)	525
DraI	TTTAAA	1 cut(s)	1564
EaeI	YGGCCR	2 cut(s)	717, 838
Eam1104I	CTCTTC	4 cut(s)	277, 315, 330, 1023
EarI	CTCTTC	4 cut(s)	277, 315, 330, 1023
Ecl136II	GAGCTC	1 cut(s)	173
Eco130I	CCWWGG	2 cut(s)	1132, 1209
Eco24I	GRGCYC	3 cut(s)	175, 276, 392
Eco31I	GGTCTC	2 cut(s)	201, 1725
Eco47I	GGWCC	2 cut(s)	1318, 1568
Eco53kI	GAGCTC	1 cut(s)	173
Eco57I	CTGAAG	4 cut(s)	525, 652, 1368, 1530
EcoICRI	GAGCTC	1 cut(s)	173
EcoRII	CCWGG	2 cut(s)	1320, 1674
EcoT14I	CCWWGG	2 cut(s)	1132, 1209
EcoT38I	GRGCYC	3 cut(s)	175, 276, 392
ErhI	CCWWGG	2 cut(s)	1132, 1209
FaeI	CATG	4 cut(s)	414, 940, 1073, 1167
FalI	AAGNNNNNCTT	6 cut(s)	294, 326, 335, 367, 1773, 1805
FaqI	GGGAC	3 cut(s)	491, 878, 950
FatI	CATG	4 cut(s)	410, 936, 1069, 1163
FauNDI	CATATG	1 cut(s)	1357
FbaI	TGATCA	1 cut(s)	1155
Fnu4HI	GCNGC	4 cut(s)	162, 441, 1149, 1353
FokI	GGATG	4 cut(s)	243, 775, 1190, 1379
FriOI	GRGCYC	3 cut(s)	175, 276, 392
Fsp4HI	GCNGC	4 cut(s)	162, 441, 1149, 1353
FspBI	CTAG	6 cut(s)	513, 776, 890, 986, 1025, 1535
GlaI	GCGC	1 cut(s)	1739
GluI	GCNGC	4 cut(s)	162, 441, 1149, 1353
GsaI	CCCAGC	2 cut(s)	431, 1633
HaeIII	GGCC	3 cut(s)	553, 719, 840
HapII	CCGG	2 cut(s)	420, 1600
HgaI	GACGC	1 cut(s)	1742
HhaI	GCGC	1 cut(s)	1740
Hin1II	CATG	4 cut(s)	414, 940, 1073, 1167
Hin6I	GCGC	1 cut(s)	1738
HinP1I	GCGC	1 cut(s)	1738
HincII	GTYRAC	1 cut(s)	1431
HindII	GTYRAC	1 cut(s)	1431
HindIII	AAGCTT	4 cut(s)	753, 930, 1276, 1639
HinfI	GANTC	5 cut(s)	121, 710, 1412, 1432, 1506
HpaII	CCGG	2 cut(s)	420, 1600
HphI	GGTGA	6 cut(s)	29, 59, 453, 923, 1196, 1529
Hpy166II	GTNNAC	5 cut(s)	349, 800, 991, 1049, 1431
Hpy188I	TCNGA	9 cut(s)	63, 157, 170, 793, 1109, 1195, 1407, 1549, 1591
Hpy8I	GTNNAC	5 cut(s)	349, 800, 991, 1049, 1431
Hpy99I	CGWCG	1 cut(s)	170
HpyAV	CCTTC	6 cut(s)	132, 262, 337, 519, 908, 1603
HpyCH4III	ACNGT	5 cut(s)	571, 804, 884, 1373, 1662
HpyCH4IV	ACGT	3 cut(s)	867, 1524, 1711
HpyCH4V	TGCA	8 cut(s)	23, 443, 725, 957, 1003, 1095, 1355, 1830
HpyF3I	CTNAG	1 cut(s)	525
HpySE526I	ACGT	3 cut(s)	867, 1524, 1711
Hsp92II	CATG	4 cut(s)	414, 940, 1073, 1167
HspAI	GCGC	1 cut(s)	1738
KroI	GCCGGC	1 cut(s)	419
KroNI	GCCGGC	1 cut(s)	421
Ksp22I	TGATCA	1 cut(s)	1155
LmnI	GCTCC	5 cut(s)	170, 271, 310, 853, 1087
Lsp1109I	GCAGC	4 cut(s)	148, 427, 1135, 1339
LweI	GCATC	9 cut(s)	10, 148, 161, 355, 427, 712, 788, 1135, 1848
MabI	ACCWGGT	1 cut(s)	1674
MaeI	CTAG	6 cut(s)	513, 776, 890, 986, 1025, 1535
MaeII	ACGT	3 cut(s)	867, 1524, 1711
MaeIII	GTNAC	5 cut(s)	884, 1324, 1417, 1615, 1837
MfeI	CAATTG	1 cut(s)	969
MflI	RGATCY	3 cut(s)	808, 1267, 1549
MhlI	GDGCHC	6 cut(s)	119, 175, 276, 392, 461, 823
MlsI	TGGCCA	1 cut(s)	840
MluNI	TGGCCA	1 cut(s)	840
MlyI	GAGTC	2 cut(s)	115, 1426
MmeI	TCCRAC	2 cut(s)	148, 1385
Mox20I	TGGCCA	1 cut(s)	840
MroNI	GCCGGC	1 cut(s)	419
MroXI	GAANNNNTTC	1 cut(s)	1785
MscI	TGGCCA	1 cut(s)	840
MseI	TTAA	7 cut(s)	827, 854, 900, 1053, 1061, 1400, 1563
Msp20I	TGGCCA	1 cut(s)	840
MspI	CCGG	2 cut(s)	420, 1600
MspR9I	CCNGG	2 cut(s)	1322, 1676
MunI	CAATTG	1 cut(s)	969
MvaI	CCWGG	2 cut(s)	1322, 1676
NaeI	GCCGGC	1 cut(s)	421
NdeI	CATATG	1 cut(s)	1357
NgoMIV	GCCGGC	1 cut(s)	419
NlaIII	CATG	4 cut(s)	414, 940, 1073, 1167
NlaIV	GGNNCC	3 cut(s)	273, 820, 849
NmuCI	GTSAC	1 cut(s)	884
NspV	TTCGAA	1 cut(s)	333
PcsI	WCGNNNNNNNCGW	1 cut(s)	110
PdiI	GCCGGC	1 cut(s)	421
PdmI	GAANNNNTTC	1 cut(s)	1785
PfeI	GAWTC	3 cut(s)	710, 1412, 1506
PflMI	CCANNNNNTGG	1 cut(s)	974
PkrI	GCNGC	4 cut(s)	163, 442, 1150, 1354
Ple19I	CGATCG	1 cut(s)	34
PleI	GAGTC	2 cut(s)	115, 1426
PpsI	GAGTC	2 cut(s)	115, 1426
PshAI	GACNNNNGTC	1 cut(s)	1764
Psp124BI	GAGCTC	1 cut(s)	175
Psp1406I	AACGTT	1 cut(s)	1711
Psp6I	CCWGG	2 cut(s)	1320, 1674
PspFI	CCCAGC	2 cut(s)	427, 1629
PspGI	CCWGG	2 cut(s)	1320, 1674
PspN4I	GGNNCC	3 cut(s)	273, 820, 849
PspPI	GGNCC	2 cut(s)	1318, 1568
PstNI	CAGNNNCTG	1 cut(s)	1553
PsuI	RGATCY	3 cut(s)	808, 1267, 1549
PvuI	CGATCG	1 cut(s)	34
RsaI	GTAC	6 cut(s)	326, 350, 649, 1048, 1527, 1659
RsaNI	GTAC	6 cut(s)	325, 349, 648, 1047, 1526, 1658
SacI	GAGCTC	1 cut(s)	175
SaqAI	TTAA	7 cut(s)	827, 854, 900, 1053, 1061, 1400, 1563
SatI	GCNGC	4 cut(s)	162, 441, 1149, 1353
Sau96I	GGNCC	2 cut(s)	1318, 1568
SchI	GAGTC	2 cut(s)	115, 1426
ScrFI	CCNGG	2 cut(s)	1322, 1676
SduI	GDGCHC	6 cut(s)	119, 175, 276, 392, 461, 823
SexAI	ACCWGGT	1 cut(s)	1674
SfaNI	GCATC	9 cut(s)	10, 148, 161, 355, 427, 712, 788, 1135, 1848
SfcI	CTRYAG	2 cut(s)	12, 1862
SfuI	TTCGAA	1 cut(s)	333
SinI	GGWCC	2 cut(s)	1318, 1568
SmlI	CTYRAG	1 cut(s)	1436
SmoI	CTYRAG	1 cut(s)	1436
SpeI	ACTAGT	1 cut(s)	985
SspMI	CTAG	6 cut(s)	513, 776, 890, 986, 1025, 1535
SstI	GAGCTC	1 cut(s)	175
StyD4I	CCNGG	2 cut(s)	1320, 1674
StyI	CCWWGG	2 cut(s)	1132, 1209
TaaI	ACNGT	5 cut(s)	571, 804, 884, 1373, 1662
TaiI	ACGT	3 cut(s)	870, 1527, 1714
TaqI	TCGA	7 cut(s)	34, 104, 119, 199, 209, 333, 1481
TatI	WGTACW	3 cut(s)	324, 348, 1657
TfiI	GAWTC	3 cut(s)	710, 1412, 1506
Tru1I	TTAA	7 cut(s)	827, 854, 900, 1053, 1061, 1400, 1563
Tru9I	TTAA	7 cut(s)	827, 854, 900, 1053, 1061, 1400, 1563
TscAI	CASTG	4 cut(s)	1235, 1388, 1769, 1810
TseFI	GTSAC	1 cut(s)	884
TseI	GCWGC	4 cut(s)	161, 440, 1148, 1352
Tsp45I	GTSAC	1 cut(s)	884
TspDTI	ATGAA	7 cut(s)	777, 925, 1194, 1304, 1314, 1622, 1704
TspGWI	ACGGA	1 cut(s)	1321
TspRI	CASTG	4 cut(s)	1235, 1388, 1769, 1810
Van91I	CCANNNNNTGG	1 cut(s)	974
VpaK11BI	GGWCC	2 cut(s)	1318, 1568
XapI	RAATTY	5 cut(s)	329, 583, 626, 680, 857
XbaI	TCTAGA	2 cut(s)	512, 1534
XcmI	CCANNNNNNNNNTGG	1 cut(s)	471
XmnI	GAANNNNTTC	1 cut(s)	1785
XspI	CTAG	6 cut(s)	513, 776, 890, 986, 1025, 1535

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.