Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0011833

Physical Location & Seq

Genomic Coordinates Forward (+)

28085978 .. 28089857

Gene Structure 3880 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1191300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2031 bp

ATGAATTCCCTGAGTCTTCTTTGTCACGCAACAATGAAACCCACCTGTAGAATCCTCAATAGATGTCGAAACTCGGCGTTTTTTGGGTTCCCACGGCCTGCAACATGGCTGCATGGTTTGACGAAGACTGGAAATTCGTCCAGTTTTTGTGTCAATTTTGAGCAAAACTGCCAATATCACGCCAACCCTTTTCGTATTTCGGGGTCTGGAGGCGTTTTCGATGATGCCCGAAAAGCCTCTCAGGTTCCCAGTTGGGGTCTTGGCCAATCTGGGATAATATCTAGGTCTTATAGTGCTGGCATTGGCACAGCTAGTAGGGGTGTTTCTGTGATTGCTAGTTTGGCATCCAGGTTTCGGAACCTTTCAACGTCAATTGAGACCCGTGTGAATGATAACAATTTCGAGAAGATATATGTTCAGGGTGGTATAAATGTGAAGCCTTTGGTGGAGAGGATTGATAAGGATGAGAACATTGTGGGAGGGGAAGAATCGAGGATAGAAGTTGGTGATGGAAAGAAATCTGAAAGCTTGAATGAAGCTACGGTTGTGACTTCGGAGAGAGAGTATTCGGATATTGAGAAGGAGGCGTGGAGGCTATTGAGGGATTCGGTTGTGACATATTGTGGGAACCCTGTGGGGACTGTGGCTGCAAATGATCCCGGTGACAAGCAACTGCTGAATTATGATCAGGTCTTTATCCGTGATTTTGTTCCATCGGCACTTGCTTTCTTGCTTAAGGGAGAAGGGGAGATTGTGAGGAACTTCCTCCTCCATACATTGCAATTGCAGAGTTGGGAGAAAACTGTGGATTGCTATAGCCCAGGACAGGGATTGATGCCTGCAAGTTTTAAAGTTAGAACTGTACCTCTCGATGGAAATAAGACTGAAGAAGTTTTAGATCCAGATTTTGGTGAATCAGCTATTGGACGTGTTGCACCTGTAGATTCTGGATTATGGTGGATTATTCTGTTGAGGGCTTACGGAAAGATAACTGGTGACTATGGATTACAGGAGAGGGTGGATGTTCAAACAGGCTTGAAAATGATTCTGAACTTGTGCTTAACTGATGGGTTTGATATGTTTCCATCTCTTTTAGTCACTGATGGTTCCTGCATGATAGACCGGCGGATGGGTATCCATGGTCATCCCCTTGAGATCCAAGCCTTATTTTACTCGGCTCTTCGATGCTCCCGTGAGATGCTTGCTGTAAATGATGGATCCAAGAATTTGGTAAGGGCCATCAACAACAGACTCAGCGCTTTGTCATTCCACATCAGAGAATACTATTGGGTGGATATGAAAAAGATGAATGAGATATATCGATATAAGACAGAAGAGTACTCTACGGAGGCTACCAACAAGTTCAATATCTACCCTGATCAAATTCCTCTATGGTTAATGGACTGGATTCCAGAAGAAGGCGGGTATTTTATTGGCAATCTACAACCAGCCCACATGGACTTTAGGTTTTTCACTCTTGGAAATCTTTGGTCCATTGTTTCATCATTGGGTACTCCAAAGCAAAATGATGCTGTATTGAATTTGATTGAAGCCAAATGGGATGATCTTGTGGGCCATATGCCACTTAAGATATCCTACCCTGCCTTGGAGTTTGAAGAATGGCGTATAATCACTGGCAGCGACCCAAAGAATACCCCATGGTCATATCATAATGGCGGATCCTGGCCGACGCTCCTGTGGCAGTTCACATTGGCATGCATCAAGATGGGCAGAATCGAACTAGCTCAGAAAGCAGCTGCTTTGGCTGAGAAGAGGCTTCGTTCTGACCGCTGGCCCGAATACTATGATACAAGGACAGGGAAGTTTATTGGAAAGCAAGCGCGGCTTTACCAAACATGGACCATTGCTGGGTTCCTTACAACTAAAATGCTGTTGGAGAATCCAGAGAAGGCGGCCTTGCTATTCTGGGACGAGGACTACGAACTTCTTGAGATCTGTGTTTGTGCACTTAGCAAGAGTGGCCGGAAAAAATGCTCCAGAGGCGCCGCTAAGTCTCAGATTCTTGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0003824 GO:0004553 GO:0004564 GO:0004575 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0005984 GO:0005985 GO:0005987 GO:0006950 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009311 GO:0009313 GO:0009636 GO:0009987 GO:0010035 GO:0015926 GO:0016052 GO:0016787 GO:0016798 GO:0022622 GO:0032501 GO:0032502 GO:0042221 GO:0042493 GO:0042542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044262 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0046352 GO:0046677 GO:0048364 GO:0048731 GO:0048856 GO:0050896 GO:0071704 GO:0090599 GO:0099402 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_hydro_100

Protein Analysis

677

Amino Acids

76.15

Weight (kDa)

6.59

Isoelectric Point (pI)

41.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_100	PF12899	196 - 635	1.8e-222	Alkaline and neutral invertase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011833)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G56560 AT3G05820 AT3G05820
fragaria_vesca	FvH4_6g07880 FvH4_6g07880 FvH4_6g07880
malus_domestica	MD04G1191300.v1.1
prunus_persica	Prupe.6G309800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0457581
rosa_laevigata	RLG00000025208
rosa_multiflora	Rmu_sc0005321.1_g000004 Rmu_sc0007404.1_g000002
rosa_roxburghii	Rroxscaffold_6G00421710
rosa_rugosa	Rorug03G0021600
rosa_samantha	Rh3AG082200 Rh3BG084500 Rh3CG084700 Rh3DG085900
rosa_wichuraiana	Rw3G007060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	2003
AccB7I	CCANNNNNTGG	1 cut(s)	908
AccII	CGCG	1 cut(s)	1843
AciI	CCGC	7 cut(s)	1126, 1422, 1677, 1789, 1843, 1913, 2007
AclWI	GGATC	7 cut(s)	650, 893, 1150, 1212, 1225, 1674, 1687
AcoI	YGGCCR	3 cut(s)	262, 1685, 1981
AcsI	RAATTY	5 cut(s)	4, 133, 1225, 1383, 1540
AcuI	CTGAAG	1 cut(s)	906
AcyI	GRCGYC	1 cut(s)	2004
AfaI	GTAC	3 cut(s)	864, 1340, 1513
AfeI	AGCGCT	1 cut(s)	1258
AflII	CTTAAG	2 cut(s)	734, 1586
AflIII	ACRYGT	1 cut(s)	928
AgsI	TTSAA	8 cut(s)	366, 532, 1028, 1039, 1366, 1540, 1550, 1616
AjiI	CACGTC	1 cut(s)	929
AjnI	CCWGG	3 cut(s)	347, 820, 1682
AjuI	GAANNNNNNNTTGG	2 cut(s)	906, 938
AluBI	AGCT	6 cut(s)	311, 528, 539, 920, 1745, 1757
AluI	AGCT	6 cut(s)	311, 528, 539, 920, 1745, 1757
Alw21I	GWGCWC	1 cut(s)	1969
Alw26I	GTCTC	2 cut(s)	371, 2019
Alw44I	GTGCAC	1 cut(s)	1965
AlwI	GGATC	7 cut(s)	650, 893, 1150, 1212, 1225, 1674, 1687
Aor51HI	AGCGCT	1 cut(s)	1258
AoxI	GGCC	8 cut(s)	95, 262, 1236, 1573, 1685, 1793, 1914, 1981
ApaLI	GTGCAC	1 cut(s)	1965
ApeKI	GCWGC	5 cut(s)	109, 647, 1638, 1754, 1757
ApoI	RAATTY	5 cut(s)	4, 133, 1225, 1383, 1540
ArsI	GACNNNNNNTTYG	2 cut(s)	118, 150
AspLEI	GCGC	3 cut(s)	1259, 1843, 2006
AspS9I	GGNCC	5 cut(s)	1236, 1491, 1573, 1794, 1860
AsuC2I	CCSGG	1 cut(s)	660
AsuHPI	GGTGA	4 cut(s)	518, 674, 923, 1007
AvaII	GGWCC	2 cut(s)	1491, 1860
BaeGI	GKGCMC	1 cut(s)	1969
BalI	TGGCCA	1 cut(s)	264
BamHI	GGATCC	2 cut(s)	1217, 1679
BanI	GGYRCC	1 cut(s)	2003
BbsI	GAAGAC	2 cut(s)	8, 131
Bbv12I	GWGCWC	1 cut(s)	1969
BbvI	GCAGC	5 cut(s)	96, 634, 1650, 1744, 1766
BceAI	ACGGC	1 cut(s)	110
BciT130I	CCWGG	3 cut(s)	349, 822, 1684
BciVI	GTATCC	1 cut(s)	1145
BclI	TGATCA	2 cut(s)	685, 1378
BcnI	CCSGG	1 cut(s)	660
BcoDI	GTCTC	2 cut(s)	371, 2019
BfaI	CTAG	4 cut(s)	282, 312, 336, 1742
BfmI	CTRYAG	3 cut(s)	46, 814, 939
BfoI	RGCGCY	2 cut(s)	1260, 2007
BfrI	CTTAAG	2 cut(s)	734, 1586
BfuI	GTATCC	1 cut(s)	1145
BglII	AGATCT	1 cut(s)	1953
BisI	GCNGC	8 cut(s)	110, 648, 1639, 1755, 1758, 1844, 1914, 2007
BlsI	GCNGC	8 cut(s)	111, 649, 1640, 1756, 1759, 1845, 1915, 2008
BmcAI	AGTACT	1 cut(s)	1340
Bme1390I	CCNGG	4 cut(s)	349, 660, 822, 1684
Bme18I	GGWCC	2 cut(s)	1491, 1860
BmgBI	CACGTC	1 cut(s)	929
BmgT120I	GGNCC	5 cut(s)	1236, 1491, 1573, 1794, 1860
BmiI	GGNNCC	9 cut(s)	89, 246, 359, 629, 1108, 1219, 1681, 1874, 2005
BmrFI	CCNGG	4 cut(s)	349, 660, 822, 1684
BmrI	ACTGGG	1 cut(s)	243
BmsI	GCATC	7 cut(s)	214, 353, 825, 1175, 1188, 1519, 1728
BmuI	ACTGGG	1 cut(s)	243
BpiI	GAAGAC	2 cut(s)	8, 131
BpmI	CTGGAG	2 cut(s)	228, 1981
BpuEI	CTTGAG	2 cut(s)	1172, 1970
BpuMI	CCSGG	1 cut(s)	660
Bsa29I	ATCGAT	1 cut(s)	1321
BsaBI	GATNNNNATC	1 cut(s)	1133
BsaHI	GRCGYC	1 cut(s)	2004
BsaI	GGTCTC	1 cut(s)	371
BsaJI	CCNNGG	5 cut(s)	92, 820, 1138, 1605, 1658
BsaXI	ACNNNNNCTCC	2 cut(s)	548, 578
Bse118I	RCCGGY	1 cut(s)	1122
Bse1I	ACTGG	6 cut(s)	133, 141, 249, 997, 1409, 1639
Bse3DI	GCAATG	2 cut(s)	776, 1863
Bse8I	GATNNNNATC	1 cut(s)	1133
BseBI	CCWGG	3 cut(s)	349, 822, 1684
BseCI	ATCGAT	1 cut(s)	1321
BseDI	CCNNGG	5 cut(s)	92, 820, 1138, 1605, 1658
BseGI	GGATG	6 cut(s)	344, 469, 1027, 1134, 1144, 1567
BseJI	GATNNNNATC	1 cut(s)	1133
BseMI	GCAATG	2 cut(s)	776, 1863
BseMII	CTCAG	5 cut(s)	254, 1267, 1758, 1760, 2030
BseNI	ACTGG	6 cut(s)	133, 141, 249, 997, 1409, 1639
BseRI	GAGGAG	1 cut(s)	758
BseSI	GKGCMC	1 cut(s)	1969
BseXI	GCAGC	5 cut(s)	96, 634, 1650, 1744, 1766
BseYI	CCCAGC	1 cut(s)	1868
Bsh1236I	CGCG	1 cut(s)	1843
BshFI	GGCC	8 cut(s)	97, 264, 1238, 1575, 1687, 1795, 1916, 1983
BshNI	GGYRCC	1 cut(s)	2003
BshVI	ATCGAT	1 cut(s)	1321
BsiHKAI	GWGCWC	1 cut(s)	1969
BsiSI	CCGG	3 cut(s)	660, 1123, 1984
BslFI	GGGAC	2 cut(s)	652, 1943
BsmAI	GTCTC	2 cut(s)	371, 2019
BsmFI	GGGAC	2 cut(s)	652, 1943
BsnI	GGCC	8 cut(s)	97, 264, 1238, 1575, 1687, 1795, 1916, 1983
Bso31I	GGTCTC	1 cut(s)	371
Bsp1286I	GDGCHC	1 cut(s)	1969
Bsp143I	GATC	9 cut(s)	655, 685, 898, 1155, 1217, 1378, 1564, 1679, 1953
Bsp19I	CCATGG	2 cut(s)	1138, 1658
BspACI	CCGC	7 cut(s)	1126, 1422, 1677, 1789, 1843, 1913, 2007
BspANI	GGCC	8 cut(s)	97, 264, 1238, 1575, 1687, 1795, 1916, 1983
BspCNI	CTCAG	5 cut(s)	253, 1266, 1759, 1759, 2029
BspDI	ATCGAT	1 cut(s)	1321
BspFNI	CGCG	1 cut(s)	1843
BspLI	GGNNCC	9 cut(s)	89, 246, 359, 629, 1108, 1219, 1681, 1874, 2005
BspPI	GGATC	7 cut(s)	650, 893, 1150, 1212, 1225, 1674, 1687
BspQI	GCTCTTC	1 cut(s)	1185
BspT107I	GGYRCC	1 cut(s)	2003
BspTI	CTTAAG	2 cut(s)	734, 1586
BspTNI	GGTCTC	1 cut(s)	371
BsrDI	GCAATG	2 cut(s)	776, 1863
BsrFI	RCCGGY	1 cut(s)	1122
BsrI	ACTGG	6 cut(s)	133, 141, 249, 997, 1409, 1639
BssAI	RCCGGY	1 cut(s)	1122
BssECI	CCNNGG	5 cut(s)	92, 820, 1138, 1605, 1658
BssMI	GATC	9 cut(s)	655, 685, 898, 1155, 1217, 1378, 1564, 1679, 1953
BssNI	GRCGYC	1 cut(s)	2004
BssT1I	CCWWGG	3 cut(s)	1138, 1605, 1658
Bst2UI	CCWGG	3 cut(s)	349, 822, 1684
Bst4CI	ACNGT	4 cut(s)	544, 643, 805, 862
Bst6I	CTCTTC	3 cut(s)	1185, 1329, 1766
BstACI	GRCGYC	1 cut(s)	2004
BstAFI	CTTAAG	2 cut(s)	734, 1586
BstC8I	GCNNGC	7 cut(s)	99, 298, 840, 1203, 1717, 1793, 1839
BstDEI	CTNAG	8 cut(s)	11, 240, 1253, 1746, 1767, 1970, 2010, 2016
BstDSI	CCRYGG	3 cut(s)	92, 1138, 1658
BstF5I	GGATG	6 cut(s)	344, 469, 1027, 1134, 1144, 1567
BstFNI	CGCG	1 cut(s)	1843
BstH2I	RGCGCY	2 cut(s)	1260, 2007
BstHHI	GCGC	3 cut(s)	1259, 1843, 2006
BstKTI	GATC	9 cut(s)	658, 688, 901, 1158, 1220, 1381, 1567, 1682, 1956
BstMAI	GTCTC	2 cut(s)	371, 2019
BstMBI	GATC	9 cut(s)	655, 685, 898, 1155, 1217, 1378, 1564, 1679, 1953
BstMWI	GCNNNNNNNGC	8 cut(s)	233, 341, 1699, 1751, 1763, 1843, 1980, 2001
BstNI	CCWGG	3 cut(s)	349, 822, 1684
BstNSI	RCATGY	1 cut(s)	1719
BstSCI	CCNGG	4 cut(s)	347, 658, 820, 1682
BstSFI	CTRYAG	3 cut(s)	46, 814, 939
BstSLI	GKGCMC	1 cut(s)	1969
BstUI	CGCG	1 cut(s)	1843
BstV1I	GCAGC	5 cut(s)	96, 634, 1650, 1744, 1766
BstV2I	GAAGAC	2 cut(s)	8, 131
BstX2I	RGATCY	5 cut(s)	898, 1155, 1217, 1679, 1953
BstXI	CCANNNNNNTGG	1 cut(s)	1228
BstYI	RGATCY	5 cut(s)	898, 1155, 1217, 1679, 1953
Bsu15I	ATCGAT	1 cut(s)	1321
BsuI	GTATCC	1 cut(s)	1145
BsuRI	GGCC	8 cut(s)	97, 264, 1238, 1575, 1687, 1795, 1916, 1983
BsuTUI	ATCGAT	1 cut(s)	1321
BtgI	CCRYGG	3 cut(s)	92, 1138, 1658
BtrI	CACGTC	1 cut(s)	929
BtsCI	GGATG	6 cut(s)	344, 469, 1027, 1134, 1144, 1567
BtsIMutI	CAGTG	2 cut(s)	1098, 1632
Cac8I	GCNNGC	7 cut(s)	99, 298, 840, 1203, 1717, 1793, 1839
CfoI	GCGC	3 cut(s)	1259, 1843, 2006
Cfr10I	RCCGGY	1 cut(s)	1122
Cfr13I	GGNCC	5 cut(s)	1236, 1491, 1573, 1794, 1860
ClaI	ATCGAT	1 cut(s)	1321
CseI	GACGC	1 cut(s)	1699
Csp6I	GTAC	3 cut(s)	863, 1339, 1512
CviAII	CATG	8 cut(s)	105, 113, 1114, 1139, 1456, 1659, 1716, 1857
CviQI	GTAC	3 cut(s)	863, 1339, 1512
DdeI	CTNAG	8 cut(s)	11, 240, 1253, 1746, 1767, 1970, 2010, 2016
DinI	GGCGCC	1 cut(s)	2005
DpnI	GATC	9 cut(s)	657, 687, 900, 1157, 1219, 1380, 1566, 1681, 1955
DpnII	GATC	9 cut(s)	655, 685, 898, 1155, 1217, 1378, 1564, 1679, 1953
DraI	TTTAAA	1 cut(s)	850
EaeI	YGGCCR	3 cut(s)	262, 1685, 1981
Eam1104I	CTCTTC	3 cut(s)	1185, 1329, 1766
EarI	CTCTTC	3 cut(s)	1185, 1329, 1766
EciI	GGCGGA	2 cut(s)	1141, 1692
Eco130I	CCWWGG	3 cut(s)	1138, 1605, 1658
Eco31I	GGTCTC	1 cut(s)	371
Eco32I	GATATC	1 cut(s)	1593
Eco47I	GGWCC	2 cut(s)	1491, 1860
Eco47III	AGCGCT	1 cut(s)	1258
Eco57I	CTGAAG	1 cut(s)	906
EcoRI	GAATTC	1 cut(s)	4
EcoRII	CCWGG	3 cut(s)	347, 820, 1682
EcoRV	GATATC	1 cut(s)	1593
EcoT14I	CCWWGG	3 cut(s)	1138, 1605, 1658
EcoT22I	ATGCAT	1 cut(s)	1721
EgeI	GGCGCC	1 cut(s)	2005
EheI	GGCGCC	1 cut(s)	2005
ErhI	CCWWGG	3 cut(s)	1138, 1605, 1658
FaeI	CATG	8 cut(s)	108, 116, 1117, 1142, 1459, 1662, 1719, 1860
FalI	AAGNNNNNCTT	4 cut(s)	1830, 1862, 1901, 1933
FaqI	GGGAC	2 cut(s)	652, 1943
FatI	CATG	8 cut(s)	104, 112, 1113, 1138, 1455, 1658, 1715, 1856
FauI	CCCGC	1 cut(s)	1415
FauNDI	CATATG	1 cut(s)	1578
FbaI	TGATCA	2 cut(s)	685, 1378
Fnu4HI	GCNGC	8 cut(s)	110, 648, 1639, 1755, 1758, 1844, 1914, 2007
FokI	GGATG	6 cut(s)	331, 476, 1034, 1131, 1141, 1574
Fsp4HI	GCNGC	8 cut(s)	110, 648, 1639, 1755, 1758, 1844, 1914, 2007
FspBI	CTAG	4 cut(s)	282, 312, 336, 1742
GlaI	GCGC	3 cut(s)	1258, 1842, 2005
GluI	GCNGC	8 cut(s)	110, 648, 1639, 1755, 1758, 1844, 1914, 2007
GsaI	CCCAGC	1 cut(s)	1872
GsuI	CTGGAG	2 cut(s)	228, 1981
HaeII	RGCGCY	2 cut(s)	1260, 2007
HaeIII	GGCC	8 cut(s)	97, 264, 1238, 1575, 1687, 1795, 1916, 1983
HapII	CCGG	3 cut(s)	660, 1123, 1984
HgaI	GACGC	1 cut(s)	1699
HhaI	GCGC	3 cut(s)	1259, 1843, 2006
Hin1I	GRCGYC	1 cut(s)	2004
Hin1II	CATG	8 cut(s)	108, 116, 1117, 1142, 1459, 1662, 1719, 1860
Hin6I	GCGC	3 cut(s)	1257, 1841, 2004
HinP1I	GCGC	3 cut(s)	1257, 1841, 2004
HindIII	AAGCTT	1 cut(s)	526
HpaII	CCGG	3 cut(s)	660, 1123, 1984
HphI	GGTGA	4 cut(s)	518, 674, 923, 1007
Hpy166II	GTNNAC	2 cut(s)	1707, 1967
Hpy188I	TCNGA	9 cut(s)	357, 523, 556, 571, 1050, 1277, 1749, 1786, 2019
Hpy8I	GTNNAC	2 cut(s)	1707, 1967
Hpy99I	CGWCG	1 cut(s)	1693
HpyAV	CCTTC	4 cut(s)	574, 737, 1412, 1903
HpyCH4III	ACNGT	4 cut(s)	544, 643, 805, 862
HpyCH4IV	ACGT	2 cut(s)	368, 928
HpyF10VI	GCNNNNNNNGC	8 cut(s)	233, 341, 1699, 1751, 1763, 1843, 1980, 2001
HpyF3I	CTNAG	8 cut(s)	11, 240, 1253, 1746, 1767, 1970, 2010, 2016
HpySE526I	ACGT	2 cut(s)	368, 928
Hsp92I	GRCGYC	1 cut(s)	2004
Hsp92II	CATG	8 cut(s)	108, 116, 1117, 1142, 1459, 1662, 1719, 1860
HspAI	GCGC	3 cut(s)	1257, 1841, 2004
KasI	GGCGCC	1 cut(s)	2003
Ksp22I	TGATCA	2 cut(s)	685, 1378
Kzo9I	GATC	9 cut(s)	655, 685, 898, 1155, 1217, 1378, 1564, 1679, 1953
LguI	GCTCTTC	1 cut(s)	1185
LmnI	GCTCC	3 cut(s)	1193, 1698, 2000
Lsp1109I	GCAGC	5 cut(s)	96, 634, 1650, 1744, 1766
LweI	GCATC	7 cut(s)	214, 353, 825, 1175, 1188, 1519, 1728
MaeI	CTAG	4 cut(s)	282, 312, 336, 1742
MaeII	ACGT	2 cut(s)	368, 928
MaeIII	GTNAC	6 cut(s)	23, 547, 613, 662, 995, 1096
MalI	GATC	9 cut(s)	657, 687, 900, 1157, 1219, 1380, 1566, 1681, 1955
MboI	GATC	9 cut(s)	655, 685, 898, 1155, 1217, 1378, 1564, 1679, 1953
MfeI	CAATTG	2 cut(s)	372, 782
MflI	RGATCY	5 cut(s)	898, 1155, 1217, 1679, 1953
MhlI	GDGCHC	1 cut(s)	1969
MlsI	TGGCCA	1 cut(s)	264
MluNI	TGGCCA	1 cut(s)	264
Mly113I	GGCGCC	1 cut(s)	2004
MlyI	GAGTC	2 cut(s)	22, 1245
MmeI	TCCRAC	1 cut(s)	1875
Mox20I	TGGCCA	1 cut(s)	264
Mph1103I	ATGCAT	1 cut(s)	1721
MscI	TGGCCA	1 cut(s)	264
MseI	TTAA	5 cut(s)	735, 849, 1061, 1397, 1587
MslI	CAYNNNNRTG	2 cut(s)	1714, 1724
Msp20I	TGGCCA	1 cut(s)	264
MspA1I	CMGCKG	2 cut(s)	1757, 1791
MspCI	CTTAAG	2 cut(s)	734, 1586
MspI	CCGG	3 cut(s)	660, 1123, 1984
MspR9I	CCNGG	4 cut(s)	349, 660, 822, 1684
MunI	CAATTG	2 cut(s)	372, 782
MvaI	CCWGG	3 cut(s)	349, 822, 1684
MvnI	CGCG	1 cut(s)	1843
MwoI	GCNNNNNNNGC	8 cut(s)	233, 341, 1699, 1751, 1763, 1843, 1980, 2001
NarI	GGCGCC	1 cut(s)	2004
NciI	CCSGG	1 cut(s)	660
NcoI	CCATGG	2 cut(s)	1138, 1658
NdeI	CATATG	1 cut(s)	1578
NdeII	GATC	9 cut(s)	655, 685, 898, 1155, 1217, 1378, 1564, 1679, 1953
NlaIII	CATG	8 cut(s)	108, 116, 1117, 1142, 1459, 1662, 1719, 1860
NlaIV	GGNNCC	9 cut(s)	89, 246, 359, 629, 1108, 1219, 1681, 1874, 2005
NmeAIII	GCCGAG	2 cut(s)	53, 1154
NmuCI	GTSAC	6 cut(s)	23, 547, 613, 662, 995, 1096
NsiI	ATGCAT	1 cut(s)	1721
NspI	RCATGY	1 cut(s)	1719
PaeI	GCATGC	1 cut(s)	1719
PciSI	GCTCTTC	1 cut(s)	1185
PcsI	WCGNNNNNNNCGW	2 cut(s)	1189, 1938
PflMI	CCANNNNNTGG	1 cut(s)	908
PkrI	GCNGC	8 cut(s)	111, 649, 1640, 1756, 1759, 1845, 1915, 2008
PleI	GAGTC	2 cut(s)	21, 1245
PluTI	GGCGCC	1 cut(s)	2007
PpsI	GAGTC	2 cut(s)	21, 1245
Psp6I	CCWGG	3 cut(s)	347, 820, 1682
PspFI	CCCAGC	1 cut(s)	1868
PspGI	CCWGG	3 cut(s)	347, 820, 1682
PspN4I	GGNNCC	9 cut(s)	89, 246, 359, 629, 1108, 1219, 1681, 1874, 2005
PspPI	GGNCC	5 cut(s)	1236, 1491, 1573, 1794, 1860
PsuI	RGATCY	5 cut(s)	898, 1155, 1217, 1679, 1953
PvuII	CAGCTG	1 cut(s)	1757
RsaI	GTAC	3 cut(s)	864, 1340, 1513
RsaNI	GTAC	3 cut(s)	863, 1339, 1512
RseI	CAYNNNNRTG	2 cut(s)	1714, 1724
SapI	GCTCTTC	1 cut(s)	1185
SaqAI	TTAA	5 cut(s)	735, 849, 1061, 1397, 1587
SatI	GCNGC	8 cut(s)	110, 648, 1639, 1755, 1758, 1844, 1914, 2007
Sau3AI	GATC	9 cut(s)	655, 685, 898, 1155, 1217, 1378, 1564, 1679, 1953
Sau96I	GGNCC	5 cut(s)	1236, 1491, 1573, 1794, 1860
ScaI	AGTACT	1 cut(s)	1340
SchI	GAGTC	2 cut(s)	22, 1245
ScrFI	CCNGG	4 cut(s)	349, 660, 822, 1684
SduI	GDGCHC	1 cut(s)	1969
SfaNI	GCATC	7 cut(s)	214, 353, 825, 1175, 1188, 1519, 1728
SfcI	CTRYAG	3 cut(s)	46, 814, 939
SfoI	GGCGCC	1 cut(s)	2005
SinI	GGWCC	2 cut(s)	1491, 1860
SmiMI	CAYNNNNRTG	2 cut(s)	1714, 1724
SmlI	CTYRAG	4 cut(s)	734, 1151, 1586, 1949
SmoI	CTYRAG	4 cut(s)	734, 1151, 1586, 1949
SphI	GCATGC	1 cut(s)	1719
SsiI	CCGC	7 cut(s)	1126, 1422, 1677, 1789, 1843, 1913, 2007
SspDI	GGCGCC	1 cut(s)	2003
SspMI	CTAG	4 cut(s)	282, 312, 336, 1742
StyD4I	CCNGG	4 cut(s)	347, 658, 820, 1682
StyI	CCWWGG	3 cut(s)	1138, 1605, 1658
TaaI	ACNGT	4 cut(s)	544, 643, 805, 862
TaiI	ACGT	2 cut(s)	371, 931
TaqI	TCGA	8 cut(s)	67, 219, 402, 491, 870, 1183, 1321, 1737
TatI	WGTACW	1 cut(s)	1338
TauI	GCSGC	3 cut(s)	1846, 1916, 2009
Tru1I	TTAA	5 cut(s)	735, 849, 1061, 1397, 1587
Tru9I	TTAA	5 cut(s)	735, 849, 1061, 1397, 1587
TscAI	CASTG	2 cut(s)	1105, 1639
TseFI	GTSAC	6 cut(s)	23, 547, 613, 662, 995, 1096
TseI	GCWGC	5 cut(s)	109, 647, 1638, 1754, 1757
Tsp45I	GTSAC	6 cut(s)	23, 547, 613, 662, 995, 1096
TspDTI	ATGAA	6 cut(s)	17, 50, 549, 1313, 1322, 1491
TspGWI	ACGGA	3 cut(s)	689, 996, 1361
TspRI	CASTG	2 cut(s)	1105, 1639
Van91I	CCANNNNNTGG	1 cut(s)	908
Vha464I	CTTAAG	2 cut(s)	734, 1586
VneI	GTGCAC	1 cut(s)	1965
VpaK11BI	GGWCC	2 cut(s)	1491, 1860
XapI	RAATTY	5 cut(s)	4, 133, 1225, 1383, 1540
XceI	RCATGY	1 cut(s)	1719
XspI	CTAG	4 cut(s)	282, 312, 336, 1742
ZrmI	AGTACT	1 cut(s)	1340
Zsp2I	ATGCAT	1 cut(s)	1721

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.