Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0014032

Physical Location & Seq

Genomic Coordinates Forward (+)

28399308 .. 28402903

Gene Structure 3596 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1195300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGGCGAGCTCGAAGACTCTGTTCGCTTTCGGAGCCCTAGCCCTCCTTGCCTCGTCGGCCTTCGCAGACGACGTCGTTGTGCTCACGGAGGACAACTTCGAGAAGGAGGTCGGTCAAGATCGCGCCGCTCTCGTCGAGTTCTACGCTCCCTGGTGTGGTCACTGTAAGAAGCTTGCACCGGAGTATGAGAAGCTCGGCTCAAGTTTTAAGAAAGCAAAATCGATTTTAATCGCAAAGGTTGATTGTGATGAGCACAAGGGCGTTTGTGGCAAGTATGGAGTTTCCGGCTACCCCACAATTCAATGGTTTCCTAAAGGGTCCCTAGAACCCAAAAAGTATGAAGGTGCCCGTAATGCAGAAGCTCTTGCTGAGTTTGTGAATAAGGAAGGAGGGACCAATGTGAAGATTGCTGCAGCCCCATCCCATGTTGTGGTACTTACGCAGGATAACTTTAATGAGGTTGTCCTGGATGAAACAAAAGATGTGCTGGTGGAGTTCTATGCACCATGGTGTGGACATTGCAAATCCCTTGCTCCTACTTATGAGAAGGTTGCCTCAGCATTTAAGTTGGAGGACGGTGTGGTGATTGCTAATCTTGATGCTGACAAATACAAGGATTTAGCTGAAAAGTACGGTGTTAGTGGATATCCCACATTGAAATTCTTCCCAAAGAACAAGAAGGAGGGTGAAGAGTATGGTGGCGGCAGAGATTTGGAGGATTTTGTAGATTTCATTAATGAGAAATCTGGCTCCAGCAGAGACGCAAAAGGACAACTTACTTCCAAAGCTGGCATTGTTGAAAATTTGGACGAATTAGTAAAGGAGTTTGTGAAATCTAGCGACGATGAGAAGAAGGCAATCTTTTCCAAGATAGAAGAGGAAGTTGGGAAACTTGAGGGTTCGGCTGCAAGATACGGTAACATATACTTGAAAGCTGCCAAAAAGTCTTTGGAGAAAGGTGCTGATTATGCAAAGAATGAAATTCAGCGACTTGAGCGCATACTCGAAAAGTCTGTCAACCCAATAAAGGCCGACGAGTTTACTCTCAAGAAGAACATTCTATACACCTTTGCTTCATCTTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003756 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0006457 GO:0006950 GO:0007275 GO:0008150 GO:0009505 GO:0009553 GO:0009566 GO:0009567 GO:0009790 GO:0009791 GO:0009793 GO:0009856 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0012505 GO:0016020 GO:0016853 GO:0016860 GO:0016864 GO:0019953 GO:0022414 GO:0030312 GO:0031090 GO:0032501 GO:0032502 GO:0033554 GO:0034976 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044706 GO:0046686 GO:0048229 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0048868 GO:0050896 GO:0051704 GO:0051716 GO:0061458 GO:0071944 GO:0098588 GO:0098805 GO:0140096

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

ERp29 Thioredoxin

Protein Analysis

362

Amino Acids

39.74

Weight (kDa)

5.57

Isoelectric Point (pI)

25.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Thioredoxin	PF00085	25 - 127	4.2e-33	Thioredoxin
Thioredoxin_2	PF13098	40 - 124	6.3e-07	Thioredoxin-like domain
OST3_OST6	PF04756	51 - 140	1.3e-08	OST3 / OST6 family, transporter family
OST3_OST6	PF04756	142 - 250	4.9e-06	OST3 / OST6 family, transporter family
Thioredoxin	PF00085	143 - 246	6.1e-33	Thioredoxin
Thioredoxin_2	PF13098	158 - 242	1e-06	Thioredoxin-like domain
ERp29	PF07749	264 - 357	6.1e-25	Endoplasmic reticulum protein ERp29, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014032)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47470 AT2G47470 AT2G47470 AT2G47470
fragaria_vesca	FvH4_6g07230
malus_domestica	MD04G1195300.v1.1 MD12G1207400.v1.1
prunus_persica	Prupe.6G315000_v2.0.a1
pyrus_communis	pycom04g17250
rosa_chinensis	RchiOBHm_Chr3g0456861
rosa_laevigata	RLG00000025273
rosa_multiflora	Rmu_sc0000133.1_g000018
rosa_roxburghii	Rroxscaffold_6G00422430
rosa_rugosa	Rorug03G0016400
rosa_samantha	Rh3BG078600 Rh3CG078400
rosa_wichuraiana	Rw3G006590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	75
AccB1I	GGYRCC	1 cut(s)	344
AccB7I	CCANNNNNTGG	2 cut(s)	430, 512
AccBSI	CCGCTC	1 cut(s)	128
AccII	CGCG	1 cut(s)	123
AciI	CCGC	2 cut(s)	126, 702
AcsI	RAATTY	3 cut(s)	659, 802, 981
AcyI	GRCGYC	1 cut(s)	72
AfaI	GTAC	2 cut(s)	435, 632
AfiI	CCNNNNNNNGG	4 cut(s)	155, 430, 512, 1027
AgsI	TTSAA	4 cut(s)	302, 658, 800, 931
AjnI	CCWGG	2 cut(s)	149, 465
AjuI	GAANNNNNNNTTGG	2 cut(s)	867, 899
AleI	CACNNNNGTG	1 cut(s)	508
AluBI	AGCT	7 cut(s)	9, 172, 193, 362, 623, 788, 935
AluI	AGCT	7 cut(s)	9, 172, 193, 362, 623, 788, 935
Alw21I	GWGCWC	3 cut(s)	11, 84, 255
Alw26I	GTCTC	1 cut(s)	753
AoxI	GGCC	2 cut(s)	57, 1029
ApeKI	GCWGC	4 cut(s)	410, 413, 905, 935
ApoI	RAATTY	3 cut(s)	659, 802, 981
ArsI	GACNNNNNNTTYG	2 cut(s)	999, 1031
AseI	ATTAAT	1 cut(s)	735
Asp700I	GAANNNNTTC	1 cut(s)	662
AspLEI	GCGC	2 cut(s)	125, 999
AspS9I	GGNCC	2 cut(s)	318, 393
AsuHPI	GGTGA	2 cut(s)	595, 698
AvaII	GGWCC	2 cut(s)	318, 393
BaeGI	GKGCMC	1 cut(s)	349
BanI	GGYRCC	1 cut(s)	344
BanII	GRGCYC	2 cut(s)	11, 37
BbsI	GAAGAC	1 cut(s)	20
Bbv12I	GWGCWC	3 cut(s)	11, 84, 255
BbvCI	CCTCAGC	1 cut(s)	556
BbvI	GCAGC	4 cut(s)	397, 425, 892, 922
BccI	CCATC	1 cut(s)	427
BciT130I	CCWGG	2 cut(s)	151, 467
BcoDI	GTCTC	1 cut(s)	753
BfaI	CTAG	3 cut(s)	38, 323, 837
BfmI	CTRYAG	1 cut(s)	411
BglI	GCCNNNNNGGC	1 cut(s)	56
BisI	GCNGC	6 cut(s)	126, 411, 414, 703, 906, 936
BlsI	GCNGC	6 cut(s)	127, 412, 415, 704, 907, 937
Bme1390I	CCNGG	2 cut(s)	151, 467
Bme18I	GGWCC	2 cut(s)	318, 393
BmgT120I	GGNCC	2 cut(s)	318, 393
BmiI	GGNNCC	6 cut(s)	34, 319, 320, 346, 394, 751
BmrFI	CCNGG	2 cut(s)	151, 467
BmsI	GCATC	1 cut(s)	589
BpiI	GAAGAC	1 cut(s)	20
BpmI	CTGGAG	1 cut(s)	736
Bpu10I	CCTNAGC	1 cut(s)	556
BpuEI	CTTGAG	4 cut(s)	184, 914, 1013, 1031
Bsa29I	ATCGAT	1 cut(s)	221
BsaBI	GATNNNNATC	1 cut(s)	227
BsaHI	GRCGYC	1 cut(s)	72
BsaJI	CCNNGG	2 cut(s)	149, 506
BsaWI	WCCGGW	1 cut(s)	178
Bsc4I	CCNNNNNNNGG	4 cut(s)	155, 430, 512, 1027
Bse3DI	GCAATG	1 cut(s)	517
Bse8I	GATNNNNATC	1 cut(s)	227
BseBI	CCWGG	2 cut(s)	151, 467
BseCI	ATCGAT	1 cut(s)	221
BseDI	CCNNGG	2 cut(s)	149, 506
BseGI	GGATG	2 cut(s)	419, 475
BseJI	GATNNNNATC	1 cut(s)	227
BseLI	CCNNNNNNNGG	4 cut(s)	155, 430, 512, 1027
BseMI	GCAATG	1 cut(s)	517
BseMII	CTCAG	2 cut(s)	360, 570
BseSI	GKGCMC	1 cut(s)	349
BseXI	GCAGC	4 cut(s)	397, 425, 892, 922
Bsh1236I	CGCG	1 cut(s)	123
BshFI	GGCC	2 cut(s)	59, 1031
BshNI	GGYRCC	1 cut(s)	344
BshVI	ATCGAT	1 cut(s)	221
BsiHKAI	GWGCWC	3 cut(s)	11, 84, 255
BsiSI	CCGG	2 cut(s)	179, 285
BslFI	GGGAC	2 cut(s)	304, 406
BslI	CCNNNNNNNGG	4 cut(s)	155, 430, 512, 1027
BsmAI	GTCTC	1 cut(s)	753
BsmBI	CGTCTC	1 cut(s)	753
BsmFI	GGGAC	2 cut(s)	304, 406
BsnI	GGCC	2 cut(s)	59, 1031
Bsp1286I	GDGCHC	5 cut(s)	11, 37, 84, 255, 349
Bsp143I	GATC	1 cut(s)	118
Bsp19I	CCATGG	1 cut(s)	506
BspACI	CCGC	2 cut(s)	126, 702
BspANI	GGCC	2 cut(s)	59, 1031
BspCNI	CTCAG	2 cut(s)	361, 569
BspDI	ATCGAT	1 cut(s)	221
BspFNI	CGCG	1 cut(s)	123
BspHI	TCATGA	1 cut(s)	1082
BspLI	GGNNCC	6 cut(s)	34, 319, 320, 346, 394, 751
BspMAI	CTGCAG	1 cut(s)	415
BspT107I	GGYRCC	1 cut(s)	344
BsrBI	CCGCTC	1 cut(s)	128
BsrDI	GCAATG	1 cut(s)	517
BssECI	CCNNGG	2 cut(s)	149, 506
BssMI	GATC	1 cut(s)	118
BssNI	GRCGYC	1 cut(s)	72
BssT1I	CCWWGG	1 cut(s)	506
Bst2UI	CCWGG	2 cut(s)	151, 467
Bst4CI	ACNGT	4 cut(s)	164, 578, 635, 917
Bst6I	CTCTTC	2 cut(s)	684, 870
BstACI	GRCGYC	1 cut(s)	72
BstC8I	GCNNGC	3 cut(s)	7, 174, 790
BstDEI	CTNAG	2 cut(s)	369, 556
BstDSI	CCRYGG	1 cut(s)	506
BstF5I	GGATG	2 cut(s)	419, 475
BstFNI	CGCG	1 cut(s)	123
BstHHI	GCGC	2 cut(s)	125, 999
BstKTI	GATC	1 cut(s)	121
BstMAI	GTCTC	1 cut(s)	753
BstMBI	GATC	1 cut(s)	118
BstMWI	GCNNNNNNNGC	7 cut(s)	32, 47, 56, 267, 353, 968, 994
BstNI	CCWGG	2 cut(s)	151, 467
BstSCI	CCNGG	2 cut(s)	149, 465
BstSFI	CTRYAG	1 cut(s)	411
BstSLI	GKGCMC	1 cut(s)	349
BstUI	CGCG	1 cut(s)	123
BstV1I	GCAGC	4 cut(s)	397, 425, 892, 922
BstV2I	GAAGAC	1 cut(s)	20
Bsu15I	ATCGAT	1 cut(s)	221
BsuRI	GGCC	2 cut(s)	59, 1031
BsuTUI	ATCGAT	1 cut(s)	221
BtgI	CCRYGG	1 cut(s)	506
BtsCI	GGATG	2 cut(s)	419, 475
BtsIMutI	CAGTG	1 cut(s)	160
Cac8I	GCNNGC	3 cut(s)	7, 174, 790
CciI	TCATGA	1 cut(s)	1082
CfoI	GCGC	2 cut(s)	125, 999
Cfr13I	GGNCC	2 cut(s)	318, 393
ClaI	ATCGAT	1 cut(s)	221
CseI	GACGC	1 cut(s)	770
Csp6I	GTAC	2 cut(s)	434, 631
CviAII	CATG	3 cut(s)	425, 507, 1083
CviQI	GTAC	2 cut(s)	434, 631
DdeI	CTNAG	2 cut(s)	369, 556
DpnI	GATC	1 cut(s)	120
DpnII	GATC	1 cut(s)	118
Eam1104I	CTCTTC	2 cut(s)	684, 870
EarI	CTCTTC	2 cut(s)	684, 870
Ecl136II	GAGCTC	1 cut(s)	9
Eco130I	CCWWGG	1 cut(s)	506
Eco24I	GRGCYC	2 cut(s)	11, 37
Eco32I	GATATC	1 cut(s)	647
Eco47I	GGWCC	2 cut(s)	318, 393
Eco53kI	GAGCTC	1 cut(s)	9
EcoICRI	GAGCTC	1 cut(s)	9
EcoO109I	RGGNCCY	1 cut(s)	318
EcoRII	CCWGG	2 cut(s)	149, 465
EcoRV	GATATC	1 cut(s)	647
EcoT14I	CCWWGG	1 cut(s)	506
EcoT38I	GRGCYC	2 cut(s)	11, 37
ErhI	CCWWGG	1 cut(s)	506
Esp3I	CGTCTC	1 cut(s)	753
FaeI	CATG	3 cut(s)	428, 510, 1086
FalI	AAGNNNNNCTT	4 cut(s)	759, 791, 845, 877
FaqI	GGGAC	2 cut(s)	304, 406
FatI	CATG	3 cut(s)	424, 506, 1082
Fnu4HI	GCNGC	6 cut(s)	126, 411, 414, 703, 906, 936
FokI	GGATG	2 cut(s)	406, 482
FriOI	GRGCYC	2 cut(s)	11, 37
Fsp4HI	GCNGC	6 cut(s)	126, 411, 414, 703, 906, 936
FspBI	CTAG	3 cut(s)	38, 323, 837
GlaI	GCGC	2 cut(s)	124, 998
GluI	GCNGC	6 cut(s)	126, 411, 414, 703, 906, 936
GsuI	CTGGAG	1 cut(s)	736
HaeIII	GGCC	2 cut(s)	59, 1031
HapII	CCGG	2 cut(s)	179, 285
HgaI	GACGC	1 cut(s)	770
HhaI	GCGC	2 cut(s)	125, 999
Hin1I	GRCGYC	1 cut(s)	72
Hin1II	CATG	3 cut(s)	428, 510, 1086
Hin6I	GCGC	2 cut(s)	123, 997
HinP1I	GCGC	2 cut(s)	123, 997
HincII	GTYRAC	1 cut(s)	1018
HindII	GTYRAC	1 cut(s)	1018
HindIII	AAGCTT	1 cut(s)	170
HinfI	GANTC	1 cut(s)	16
HpaII	CCGG	2 cut(s)	179, 285
HphI	GGTGA	2 cut(s)	595, 698
Hpy166II	GTNNAC	3 cut(s)	515, 1018, 1041
Hpy188I	TCNGA	1 cut(s)	32
Hpy188III	TCNNGA	5 cut(s)	100, 116, 596, 1048, 1083
Hpy8I	GTNNAC	3 cut(s)	515, 1018, 1041
Hpy99I	CGWCG	6 cut(s)	58, 74, 77, 137, 845, 1037
HpyAV	CCTTC	7 cut(s)	70, 97, 335, 380, 541, 673, 847
HpyCH4III	ACNGT	4 cut(s)	164, 578, 635, 917
HpyCH4IV	ACGT	1 cut(s)	72
HpyCH4V	TGCA	7 cut(s)	176, 356, 413, 503, 522, 908, 971
HpyF10VI	GCNNNNNNNGC	7 cut(s)	32, 47, 56, 267, 353, 968, 994
HpyF3I	CTNAG	2 cut(s)	369, 556
HpySE526I	ACGT	1 cut(s)	72
Hsp92I	GRCGYC	1 cut(s)	72
Hsp92II	CATG	3 cut(s)	428, 510, 1086
HspAI	GCGC	2 cut(s)	123, 997
KflI	GGGWCCC	1 cut(s)	318
Kzo9I	GATC	1 cut(s)	118
LmnI	GCTCC	4 cut(s)	32, 151, 538, 755
Lsp1109I	GCAGC	4 cut(s)	397, 425, 892, 922
LweI	GCATC	1 cut(s)	589
MaeI	CTAG	3 cut(s)	38, 323, 837
MaeII	ACGT	1 cut(s)	72
MaeIII	GTNAC	2 cut(s)	158, 917
MalI	GATC	1 cut(s)	120
MbiI	CCGCTC	1 cut(s)	128
MboI	GATC	1 cut(s)	118
MboII	GAAGA	8 cut(s)	25, 415, 655, 701, 862, 887, 1063, 1071
MhlI	GDGCHC	5 cut(s)	11, 37, 84, 255, 349
MluCI	AATT	5 cut(s)	297, 659, 802, 812, 981
MlyI	GAGTC	1 cut(s)	10
MmeI	TCCRAC	1 cut(s)	549
MroXI	GAANNNNTTC	1 cut(s)	662
MseI	TTAA	5 cut(s)	207, 227, 453, 564, 735
MslI	CAYNNNNRTG	2 cut(s)	508, 1081
MspI	CCGG	2 cut(s)	179, 285
MspR9I	CCNGG	2 cut(s)	151, 467
MvaI	CCWGG	2 cut(s)	151, 467
MvnI	CGCG	1 cut(s)	123
MwoI	GCNNNNNNNGC	7 cut(s)	32, 47, 56, 267, 353, 968, 994
NcoI	CCATGG	1 cut(s)	506
NdeII	GATC	1 cut(s)	118
NlaIII	CATG	3 cut(s)	428, 510, 1086
NlaIV	GGNNCC	6 cut(s)	34, 319, 320, 346, 394, 751
NmeAIII	GCCGAG	1 cut(s)	174
NmuCI	GTSAC	1 cut(s)	158
OliI	CACNNNNGTG	1 cut(s)	508
PagI	TCATGA	1 cut(s)	1082
PcsI	WCGNNNNNNNCGW	1 cut(s)	69
PdmI	GAANNNNTTC	1 cut(s)	662
PflFI	GACNNNGTC	1 cut(s)	71
PflMI	CCANNNNNTGG	2 cut(s)	430, 512
PfoI	TCCNGGA	1 cut(s)	465
PkrI	GCNGC	6 cut(s)	127, 412, 415, 704, 907, 937
PleI	GAGTC	1 cut(s)	10
PpsI	GAGTC	1 cut(s)	10
PpuMI	RGGWCCY	1 cut(s)	318
PshBI	ATTAAT	1 cut(s)	735
Psp124BI	GAGCTC	1 cut(s)	11
Psp5II	RGGWCCY	1 cut(s)	318
Psp6I	CCWGG	2 cut(s)	149, 465
PspGI	CCWGG	2 cut(s)	149, 465
PspN4I	GGNNCC	6 cut(s)	34, 319, 320, 346, 394, 751
PspPI	GGNCC	2 cut(s)	318, 393
PspPPI	RGGWCCY	1 cut(s)	318
PsrI	GAACNNNNNNTAC	2 cut(s)	1046, 1078
PstI	CTGCAG	1 cut(s)	415
PsyI	GACNNNGTC	1 cut(s)	71
RsaI	GTAC	2 cut(s)	435, 632
RsaNI	GTAC	2 cut(s)	434, 631
RseI	CAYNNNNRTG	2 cut(s)	508, 1081
SacI	GAGCTC	1 cut(s)	11
SaqAI	TTAA	5 cut(s)	207, 227, 453, 564, 735
SatI	GCNGC	6 cut(s)	126, 411, 414, 703, 906, 936
Sau3AI	GATC	1 cut(s)	118
Sau96I	GGNCC	2 cut(s)	318, 393
SchI	GAGTC	1 cut(s)	10
ScrFI	CCNGG	2 cut(s)	151, 467
SduI	GDGCHC	5 cut(s)	11, 37, 84, 255, 349
SfaNI	GCATC	1 cut(s)	589
SfcI	CTRYAG	1 cut(s)	411
SinI	GGWCC	2 cut(s)	318, 393
SmiMI	CAYNNNNRTG	2 cut(s)	508, 1081
SmlI	CTYRAG	4 cut(s)	199, 893, 992, 1046
SmoI	CTYRAG	4 cut(s)	199, 893, 992, 1046
Sse9I	AATT	5 cut(s)	297, 659, 802, 812, 981
SsiI	CCGC	2 cut(s)	126, 702
SspMI	CTAG	3 cut(s)	38, 323, 837
SstI	GAGCTC	1 cut(s)	11
StyD4I	CCNGG	2 cut(s)	149, 465
StyI	CCWWGG	1 cut(s)	506
TaaI	ACNGT	4 cut(s)	164, 578, 635, 917
TaiI	ACGT	1 cut(s)	75
TaqI	TCGA	5 cut(s)	11, 99, 135, 221, 1005
TaqII	GACCGA	1 cut(s)	101
TasI	AATT	5 cut(s)	297, 659, 802, 812, 981
TauI	GCSGC	2 cut(s)	128, 705
Tru1I	TTAA	5 cut(s)	207, 227, 453, 564, 735
Tru9I	TTAA	5 cut(s)	207, 227, 453, 564, 735
TscAI	CASTG	1 cut(s)	167
TseFI	GTSAC	1 cut(s)	158
TseI	GCWGC	4 cut(s)	410, 413, 905, 935
Tsp45I	GTSAC	1 cut(s)	158
TspDTI	ATGAA	6 cut(s)	354, 486, 721, 993, 1065, 1071
TspGWI	ACGGA	1 cut(s)	101
TspRI	CASTG	1 cut(s)	167
Tth111I	GACNNNGTC	1 cut(s)	71
Van91I	CCANNNNNTGG	2 cut(s)	430, 512
VpaK11BI	GGWCC	2 cut(s)	318, 393
VspI	ATTAAT	1 cut(s)	735
XapI	RAATTY	3 cut(s)	659, 802, 981
XcmI	CCANNNNNNNNNTGG	1 cut(s)	946
XmnI	GAANNNNTTC	1 cut(s)	662
XspI	CTAG	3 cut(s)	38, 323, 837
ZraI	GACGTC	1 cut(s)	73

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.